Hemoglobin concentration detection method and blood cell analyzer

Abstract

The present invention provides a method for detecting hemoglobin concentration and a blood cell analyzer, wherein the method comprises: detecting a first test sample injected into a reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, and a first hemolytic agent; detecting a second test sample injected into the reaction cell and obtaining a second HGB value; wherein the second test sample is obtained by adding the second hemolytic agent to the first test sample in the reaction cell and mixing them; and selecting the minimum of the first and second HGB values as the HGB value of the blood sample. Because the presence of bubbles during the detection process can lead to an inflated HGB value, the present application proposes selecting the minimum of the first and second HGB values as the HGB value of the blood sample. This minimizes the impact of bubbles on the HGB value and improves the accuracy of the HGB detection result.

Description

Technical Field

[0001] The present application relates to the technical field of blood testing, and in particular to a method for detecting hemoglobin concentration and a blood cell analyzer. Background Art

[0002] Hematology analyzers can be used to detect hemoglobin concentration (HGB), which is an important parameter for diagnosing anemia.

[0003] Currently, the main process for HGB value detection is: adding diluent, blood sample and hemolytic agent to the reaction pool, irradiating the reaction pool with a light source, and using the detected voltage value as the sample voltage; adding diluent to the reaction pool, irradiating the reaction pool with a light source, and using the detected voltage value as the background voltage; and then calculating the HGB value of the blood sample based on the sample voltage and background voltage.

[0004] At present, it is inevitable that there may be bubbles in the reaction pool during the detection process. When the reaction pool containing the test sample is irradiated with light when there are bubbles in the reaction pool, the detected sample voltage will be low, and then the HGB value test result will be high, affecting the doctor's diagnosis of the disease.

[0005] Therefore, a solution is now needed to reduce the impact of bubbles on HGB value detection results and improve the accuracy of HGB value detection results. Summary of the Invention

[0006] In view of this, the present application provides a method for detecting hemoglobin concentration and a blood cell analyzer to reduce the impact of bubbles on HGB value detection results and improve the accuracy of HGB value detection results.

[0007] In order to achieve the above object, the present invention provides the following technical features:

[0008] A method for detecting hemoglobin concentration, comprising:

[0009] Detecting a first test sample injected into the reaction pool and obtaining a first HGB value; wherein the first test sample includes a diluent, a blood sample, and a first hemolytic agent;

[0010] detecting a second test sample injected into the reaction pool and obtaining a second HGB value; wherein the second test sample is obtained by adding a second hemolytic agent to the first test sample in the reaction pool and mixing;

[0011] The minimum value between the first HGB value and the second HGB value is selected as the HGB value of the blood sample.

[0012] Optionally, the detecting a first test sample injected into the reaction cell and obtaining a first HGB value, and the detecting a second test sample injected into the reaction cell and obtaining a second HGB value include:

[0013] Detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage;

[0014] The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0015] Optionally, detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; and calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes:

[0016] detecting the dilution solution injected into the reaction cell and obtaining the background voltage;

[0017] After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining a first sample voltage, and calculating the first HGB value based on the background voltage and the first sample voltage;

[0018] A second test sample injected into the reaction cell is detected to obtain a second sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0019] Optionally, detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; and calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes:

[0020] detecting a first test sample injected into the reaction cell and obtaining a first sample voltage;

[0021] detecting a second test sample injected into the reaction cell and obtaining a voltage of the second sample;

[0022] After draining the reaction cell, detecting the dilution solution injected into the reaction cell and obtaining the background voltage;

[0023] The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0024] Optionally, detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; and calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes:

[0025] detecting the dilution solution injected into the reaction cell and obtaining a first background voltage;

[0026] After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining the first sample voltage;

[0027] detecting a second test sample injected into the reaction cell and obtaining a voltage of the second sample;

[0028] After draining the reaction cell, re-detecting the dilution solution injected into the reaction cell and obtaining a second background voltage;

[0029] selecting the larger of the first background voltage and the second background voltage as the background voltage;

[0030] The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0031] Optionally, calculating the first HGB value based on the background voltage and the first sample voltage includes:

[0032] According to the formula Calculating and obtaining the first HGB value;

[0033] The calculating the second HGB value based on the background voltage and the second sample voltage includes:

[0034] According to the formula Calculating and obtaining the second HGB value;

[0035] Wherein, the first parameter K1 and the second parameter K2 are both predetermined constants;

[0036] The first parameter K1 is determined by the first hemolysis dose, the diluent volume and the blood sample volume;

[0037] The second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the diluent volume, and the blood sample volume.

[0038] Optionally, before selecting the minimum value between the first HGB value and the second HGB value as the HGB value of the blood sample, the method further includes:

[0039] Determine whether there are bubbles during the detection process;

[0040] If there are bubbles during the detection process, the minimum value between the first HGB value and the second HGB value is selected as the HGB value of the blood sample.

[0041] Optionally, determining whether bubbles are present during the detection process includes:

[0042] determining whether an absolute value of a difference between the second HGB value and the first HGB value is greater than a first preset threshold, if so, determining that bubbles are present during the detection process; otherwise, determining that no bubbles are present during the detection process;

[0043] or,

[0044] It is determined whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value. If so, it is determined that there are bubbles during the detection process; otherwise, it is determined that there are no bubbles during the detection process.

[0045] Optionally, also include:

[0046] The first test sample injected into the reaction pool is tested and white blood cell classification data is obtained.

[0047] Optionally, also include:

[0048] The second test sample injected into the reaction pool is detected and white blood cell count data is obtained.

[0049] Optionally, a first parameter K1 for calculating the first HGB value and a second parameter K2 for calculating the second HGB value are pre-stored.

[0050] Optionally, the hemoglobin concentration detection method is applied to an animal-type blood cell analyzer.

[0051] A blood cell analyzer, comprising:

[0052] reaction tank;

[0053] a diluent supply device, for providing diluent to the reaction tank;

[0054] a sample supply device, for providing a blood sample to the reaction cell;

[0055] a hemolytic agent supply device for supplying a first hemolytic agent to the reaction pool to mix with the blood sample and the diluent to obtain a first test sample, and for supplying a second hemolytic agent to the reaction pool to mix with the first test sample to obtain a second test sample;

[0056] a mixing device, for respectively mixing the first test sample or the second test sample in the reaction pool;

[0057] The detection device is used to detect the first detection sample in the reaction pool to obtain a first HGB value, and to detect the second detection sample in the reaction pool to obtain a second HGB value, and select the minimum value between the first HGB value and the second HGB value as the HGB value of the blood sample.

[0058] Optionally, the detection device includes an HGB detection module for detecting an HGB value, wherein the HGB detection module is configured to:

[0059] Detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage;

[0060] The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0061] Optionally, the detection device further includes a white blood cell detection module for detecting white blood cells in the detection sample, and the white blood cell detection module measures the optical information of the first detection sample to count the white blood cells of the blood sample.

[0062] Optionally, the white blood cell detection module is further used to measure the optical information of the second detection sample to classify white blood cells in the blood sample.

[0063] Optionally, the blood cell analyzer is applied to an animal model blood cell analyzer.

[0064] Through the above technical means, the following beneficial effects can be achieved:

[0065] The present invention can perform two hemoglobin concentration tests on a blood sample in the same reaction pool to obtain a first HGB value and a second HGB value, and select the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.

[0066] Because the presence of bubbles during the test process will cause the HGB value test result to be biased high, the present application proposes selecting the minimum value from the first HGB value and the second HGB value as the HGB value of the blood sample. This can minimize the impact of bubbles on the HGB value test result and improve the accuracy of the HGB test result. BRIEF DESCRIPTION OF THE DRAWINGS

[0067] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0068] Figure 1 A flow chart of a first embodiment of a method for detecting hemoglobin concentration provided by an embodiment of the present invention;

[0069] Figure 2 A flow chart of a first embodiment of a method for detecting hemoglobin concentration provided by an embodiment of the present invention;

[0070] Figure 3 This is a flow chart of a first implementation of a method for detecting hemoglobin concentration provided by an embodiment of the present invention;

[0071] Figure 4 This is a flow chart of a second implementation of a method for detecting hemoglobin concentration provided by an embodiment of the present invention;

[0072] Figure 5 This is a flow chart of a third implementation of a method for detecting hemoglobin concentration provided in an embodiment of the present invention;

[0073] Figure 6 A schematic structural diagram of a blood cell analyzer provided in an embodiment of the present invention;

[0074] Figure 7 This is a structural schematic diagram of a white blood cell detection device in a blood cell analyzer provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0075] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0076] The present invention provides a method for detecting hemoglobin concentration in Example 1, which is applied to a blood cell analyzer. Figure 1 , including the following steps:

[0077] Step S101: Detecting a first test sample injected into a reaction cell and obtaining a first HGB value; wherein the first test sample includes a diluent, a blood sample, and a first hemolytic agent.

[0078] After obtaining a blood sample of a certain type, the blood cell analyzer determines the ratio of the blood sample, the first hemolytic agent, and the diluent, injects the blood sample, the first hemolytic agent, and the diluent into a reaction cell according to the ratio, and mixes them to obtain a first test sample. The hemoglobin concentration of the first test sample in the reaction cell is then measured to obtain a first HGB value for the blood sample.

[0079] Optionally, the first test sample is further used to perform a leukocyte classification operation, that is, to detect the first test sample injected into the reaction pool and obtain leukocyte classification data.

[0080] Step S102: detecting a second test sample injected into the reaction cell and obtaining a second HGB value; wherein the second test sample is obtained by adding a second hemolytic agent to the first test sample in the reaction cell and mixing the mixture.

[0081] Based on the ratio of the blood sample, the first hemolytic agent, and the diluent, a specified ratio of the second hemolytic agent is determined. The second hemolytic agent is then added to the reaction cell containing the first test sample, and the mixture is mixed to obtain a second test sample. The hemoglobin concentration of the second test sample in the reaction cell is then measured to obtain a second HGB value for the blood sample. Furthermore, an appropriate amount of diluent may also be added simultaneously with the addition of the second hemolytic agent.

[0082] Optionally, the second test sample is further used to perform a white blood cell counting operation, that is, to detect the second test sample injected into the reaction pool and obtain white blood cell counting data.

[0083] Step S103: The minimum of the first and second HGB values is selected as the HGB value of the blood sample. It should be noted that the hemoglobin concentration detection device (i.e., the blood cell analyzer) is calibrated using calibrants at the factory to ensure that the first and second HGB values measured by the hemoglobin concentration detection device are consistent. Therefore, any discrepancy between the first and second HGB values is not due to an error in the hemoglobin concentration detection device, but rather to the influence of bubbles.

[0084] The present invention provides a second embodiment of a method for detecting hemoglobin concentration, which is applied to a blood cell analyzer. Based on the first embodiment, a process of determining whether bubbles are present during the detection process is added.

[0085] See also Figure 2 , including the following steps:

[0086] Step S201: Detecting a first test sample injected into a reaction cell and obtaining a first HGB value; wherein the first test sample includes a diluent, a blood sample, and a first hemolytic agent.

[0087] After obtaining a blood sample of a certain type, the blood cell analyzer determines the ratio of the blood sample, the first hemolytic agent, and the diluent, injects the blood sample, the first hemolytic agent, and the diluent into a reaction cell according to the ratio, and mixes them to obtain a first test sample. The hemoglobin concentration of the first test sample in the reaction cell is then measured to obtain a first HGB value for the blood sample.

[0088] Optionally, the first test sample is further used to perform a leukocyte classification operation, that is, to detect the first test sample injected into the reaction pool and obtain leukocyte classification data.

[0089] Step S202: detecting a second test sample injected into the reaction cell and obtaining a second HGB value; wherein the second test sample is obtained by adding a second hemolytic agent to the first test sample in the reaction cell and mixing the mixture.

[0090] Based on the ratio of the blood sample, the first hemolytic agent, and the diluent, a specified ratio of the second hemolytic agent is determined. The second hemolytic agent is added to the reaction pool containing the first test sample, and the mixture is mixed to obtain a second test sample. The hemoglobin concentration of the second test sample in the reaction pool is tested to obtain a second HGB value of the blood sample.

[0091] Optionally, the second test sample is further used to perform a white blood cell counting operation, that is, to detect the second test sample injected into the reaction pool and obtain white blood cell counting data.

[0092] Step S203: Determine whether there are bubbles during the detection process; if so, proceed to step S204.

[0093] If there are no bubbles during the detection process, an HGB value may be randomly selected from the first HGB value and the second HGB value as the HGB value of the blood sample.

[0094] This embodiment provides two implementation methods for determining whether bubbles are present during the detection process:

[0095] The first implementation manner: determining whether the absolute value of the difference between the second HGB value and the first HGB value is greater than a first preset threshold; if so, determining that there are bubbles during the detection process; otherwise, determining that there are no bubbles during the detection process.

[0096] An absolute value of a difference between the second HGB value and the first HGB value is calculated, and it is determined whether the absolute value of the difference between the second HGB value and the first HGB value is greater than a first preset threshold.

[0097] If the absolute value of the difference between the second HGB value and the first HGB value is not greater than the first preset threshold, it means that the first HGB value and the second HGB value are not much different (due to experimental error), and it is determined that there are no bubbles during the detection process; otherwise, it is determined that there are bubbles during the detection process.

[0098] The second implementation manner: determining whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold; if so, determining that there are bubbles during the detection process; otherwise, determining that there are no bubbles during the detection process.

[0099] A ratio of the second HGB value to the first HGB value is calculated, and it is determined whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold.

[0100] If the ratio of the second HGB value to the first HGB value is greater than a second preset threshold, it is determined that there are bubbles during the detection process; otherwise, it is determined that there are no bubbles during the detection process.

[0101] Step S204: selecting the minimum value between the first HGB value and the second HGB value as the HGB value of the blood sample.

[0102] Through the above technical means, the following beneficial effects can be achieved:

[0103] The present invention can perform two hemoglobin concentration tests on a blood sample in the same reaction pool to obtain a first HGB value and a second HGB value, and select the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.

[0104] Because the presence of bubbles during the test process will cause the HGB value test result to be biased high, the present application proposes selecting the minimum value from the first HGB value and the second HGB value as the HGB value of the blood sample. This can minimize the impact of bubbles on the HGB value test result and improve the accuracy of the HGB test result.

[0105] According to an embodiment provided by the present invention, steps S101 and S102 in the first embodiment and steps S201 and S202 in the second embodiment may specifically include the following operations:

[0106] The diluted liquid injected into the reaction cell is detected to obtain a background voltage, the first test sample injected into the reaction cell is detected to obtain a first sample voltage, and the second test sample injected into the reaction cell is detected to obtain a second sample voltage; the first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0107] In the first embodiment, step S101 and step S102 include three implementation methods, and the three implementation methods are described in detail below.

[0108] The first implementation method is to detect the background voltage at the beginning of the hemoglobin concentration detection.

[0109] See also Figure 3 , the first implementation method includes the following steps:

[0110] Step S301: Detecting the dilution solution injected into the reaction cell and obtaining the background voltage.

[0111] First, a diluent is injected into the reaction cell, and the background voltage of the diluent is detected for subsequent calculation of the first HGB value and the second HGB value.

[0112] Step S302 : After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining the first sample voltage, and calculating the first HGB value based on the background voltage and the first sample voltage.

[0113] After detecting the background voltage, in order not to affect the blood sample detection, the diluent in the reaction pool needs to be drained.

[0114] After obtaining a certain type of blood sample, the blood cell analyzer determines the ratio of the blood sample, the first hemolytic agent and the diluent, injects the blood sample, the first solution and the diluent into the reaction pool according to the ratio, and obtains the first test sample after mixing.

[0115] Turn on the light source, illuminate the reaction cell with the light source, and obtain a first sample voltage of the first test sample. Calculate the first HGB value based on the background voltage and the first sample voltage using the following formula:

[0116]

[0117] The first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first hemolysis dose, the diluent volume, and the blood sample volume.

[0118] Step S303: detecting the second test sample injected into the reaction cell and obtaining the second sample voltage, and calculating the second HGB value based on the background voltage and the second sample voltage.

[0119] Based on the ratio of the blood sample, the first hemolytic agent and the diluent, the specified ratio of the second hemolytic agent to be added is determined, and the second hemolytic agent of the specified ratio is added to the reaction pool containing the first test sample, and the second test sample is obtained after mixing.

[0120] Turn on the light source, illuminate the reaction cell with the light source, and obtain a second sample voltage of the second test sample. Calculate the second HGB value based on the background voltage and the second sample voltage using the following formula:

[0121]

[0122] The second parameter K2 is a predetermined constant; the second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the diluent volume, and the blood sample volume.

[0123] Optionally, the blood cell analyzer pre-stores a first parameter K1 for calculating the first HGB value and a second parameter K2 for calculating the second HGB value.

[0124] The second implementation method is to detect the background voltage at the end of performing the hemoglobin concentration detection.

[0125] See also Figure 4 , the second implementation method includes the following steps:

[0126] Step S401: detecting a first test sample injected into the reaction cell and obtaining the first sample voltage.

[0127] After obtaining a blood sample of a certain type, the blood cell analyzer determines the ratio of the blood sample, a first hemolytic agent, and a diluent, injects the blood sample, the first solution, and the diluent into a reaction cell according to the ratio, and mixes them to obtain a first test sample. A light source is then turned on to illuminate the reaction cell and obtain a first sample voltage for the first test sample.

[0128] Step S402: detecting a second test sample injected into the reaction cell and obtaining a voltage of the second sample.

[0129] Based on the ratio of the blood sample, the first hemolytic agent, and the diluent, a specified ratio of the second hemolytic agent is determined. The second hemolytic agent is added to the reaction cell containing the first test sample at the specified ratio, and the mixture is mixed to obtain a second test sample. A light source is turned on, the reaction cell is illuminated with the light source, and a second sample voltage of the second test sample is obtained.

[0130] Step S403: After draining the reaction cell, detecting the dilution solution injected into the reaction cell and obtaining the background voltage.

[0131] The second test sample in the reaction cell is drained, a diluent is injected into the reaction cell, and the background voltage of the diluent is detected for subsequent calculation of the first HGB value and the second HGB value.

[0132] Step S404: Calculate the first HGB value based on the background voltage and the first sample voltage, and calculate the second HGB value based on the background voltage and the second sample voltage.

[0133] The first HGB value is calculated based on the background voltage and the first sample voltage, and the calculation formula is as follows:

[0134]

[0135] The first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first hemolysis dose, the diluent volume, and the blood sample volume.

[0136] The second HGB value is calculated based on the background voltage and the second sample voltage, and the calculation formula is as follows:

[0137]

[0138] The second parameter K2 is a predetermined constant; the second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the diluent volume, and the blood sample volume.

[0139] Optionally, the blood cell analyzer pre-stores a first parameter K1 for calculating the first HGB value and a second parameter K2 for calculating the second HGB value.

[0140] A third implementation method is to detect the background voltage at the beginning and end of the hemoglobin concentration detection.

[0141] See also Figure 5 , the third implementation method includes the following steps:

[0142] Step S501: Detecting the dilution solution injected into the reaction cell and obtaining a first background voltage.

[0143] First, inject diluent into the reaction cell and detect the first background voltage of the diluent.

[0144] Step S502 : After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining the first sample voltage.

[0145] After detecting the background voltage, in order not to affect the blood sample detection, the diluent in the reaction pool needs to be drained.

[0146] After obtaining a blood sample of a certain type, the blood cell analyzer determines the ratio of the blood sample, a first hemolytic agent, and a diluent, injects the blood sample, the first solution, and the diluent into a reaction cell according to the ratio, and mixes them to obtain a first test sample. A light source is then turned on to illuminate the reaction cell and obtain a first sample voltage for the first test sample.

[0147] Step S503: detecting the second test sample injected into the reaction cell and obtaining the second sample voltage.

[0148] Based on the ratio of the blood sample, the first hemolytic agent, and the diluent, a specified ratio of the second hemolytic agent is determined. The second hemolytic agent is added to the reaction cell containing the first test sample at the specified ratio, and the mixture is mixed to obtain a second test sample. A light source is turned on, the reaction cell is illuminated with the light source, and a second sample voltage of the second test sample is obtained.

[0149] Step S504: After draining the reaction cell, re-detect the dilution solution injected into the reaction cell and obtain a second background voltage.

[0150] The second test sample in the reaction pool is drained, a diluent is injected into the reaction pool, and a second background voltage of the diluent is detected.

[0151] Step S505: selecting the larger one of the first background voltage and the second background voltage as the background voltage.

[0152] For greater accuracy, in this embodiment, the background voltage is also detected twice to eliminate the possibility that bubbles mixed into the background voltage detection process may affect the detection result of the hemoglobin concentration.

[0153] The hemoglobin concentration calculation formula shows that the background voltage, as a numerator, can cause the local voltage to be lower in the presence of bubbles, leading to a lower HGB value. Therefore, the larger of the first and second background voltages is selected as the background voltage to minimize the impact of bubbles.

[0154] Step S506 : Calculate the first HGB value based on the background voltage and the first sample voltage, and calculate the second HGB value based on the background voltage and the second sample voltage.

[0155] The first HGB value is calculated based on the background voltage and the first sample voltage, and the calculation formula is as follows:

[0156]

[0157] The first parameter K1 is a predetermined constant, and the first parameter K1 is determined by the first hemolysis dose, the diluent volume, and the blood sample volume.

[0158] The second HGB value is calculated based on the background voltage and the second sample voltage, and the calculation formula is as follows:

[0159]

[0160] The second parameter K2 is a predetermined constant; the second parameter K2 is determined by the first hemolysis dose, the second hemolysis dose, the diluent volume, and the blood sample volume.

[0161] Optionally, the blood cell analyzer pre-stores a first parameter K1 for calculating the first HGB value and a second parameter K2 for calculating the second HGB value.

[0162] Optionally, the hemoglobin concentration detection method provided in the present application can be applied to an animal-type blood cell analyzer to measure the hemoglobin concentration of the animal.

[0163] See also Figure 6 The present invention provides a blood cell analyzer, comprising:

[0164] reaction tank;

[0165] a diluent supply device, for providing diluent to the reaction tank;

[0166] a sample supply device, for providing a blood sample to the reaction cell;

[0167] a hemolytic agent supply device for supplying a first hemolytic agent to the reaction pool to mix with the blood sample and the diluent to obtain a first test sample, and for supplying a second hemolytic agent to the reaction pool to mix with the first test sample to obtain a second test sample;

[0168] The mixing device is used to mix the first detection sample or the second detection sample in the reaction pool respectively.

[0169] The light source device is used to emit light.

[0170] The detection device is used to detect the first detection sample in the reaction pool to obtain a first HGB value, and to detect the second detection sample in the reaction pool to obtain a second HGB value, and select the minimum value between the first HGB value and the second HGB value as the HGB value of the blood sample.

[0171] A control device is communicatively connected to the diluent supply device, the sample supply device, the hemolytic agent supply device, the mixing device, the light source device, and the detection device.

[0172] The control device is used to control the diluent supply device, the sample supply device, the hemolytic agent supply device and the mixing device to prepare the diluent, the first test sample and the second test sample, and control the light source device to turn on after the diluent preparation is completed, control the light source device to turn on after the first test sample preparation is completed, and control the light source device to turn on after the second test sample preparation is completed, so that the detection device can detect the first HGB value and the second HGB value.

[0173] The detection device includes an HGB detection module for detecting an HGB value.

[0174] The HGB detection module is used to:

[0175] Detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage;

[0176] The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

[0177] For details on the specific implementation of the HGB detection module in the blood cell analyzer, please refer to Figure 1-Figure 5 The embodiments shown are not described in detail here.

[0178] Optionally, the blood cell analyzer is applied to an animal model blood cell analyzer.

[0179] The detection device also includes a white blood cell detection module for detecting white blood cells in the detection sample.

[0180] The white blood cell detection module measures the optical information of the first detection sample to count the white blood cells of the blood sample.

[0181] The white blood cell detection module is further used to measure the optical information of the second detection sample to perform white blood cell classification on the blood sample.

[0182] See also Figure 7The present invention provides a schematic structural diagram of a white blood cell detection device. The white blood cell detection device includes an optical detection unit 131, which comprises a light source 1311, a beam shaping assembly 1312, a flow chamber 1313, and a forward scattered light detector 1314, arranged in a straight line. A dichroic mirror 1316 is arranged on one side of the flow chamber 1313 at a 45° angle to the straight line. A portion of the side light emitted by blood cells in the flow chamber 1313 passes through the dichroic mirror 1316 and is captured by a fluorescence detector 1315, which is arranged at a 45° angle behind the dichroic mirror 1316. Another portion of the side light is reflected by the dichroic mirror 1316 and captured by a side scattered light detector 1317, which is arranged at a 45° angle in front of the dichroic mirror 1316. White blood cells in the blood sample may be counted and classified based on the forward scattered light signal captured by the forward scattered light detector 1314 , the side scattered light signal captured by the side scattered light detector 1317 , and the fluorescence signal captured by the fluorescence detector 1315 .

[0183] Through the above technical means, the following beneficial effects can be achieved:

[0184] The present invention can perform two hemoglobin concentration tests on a blood sample in the same reaction pool to obtain a first HGB value and a second HGB value, and select the minimum value of the first HGB value and the second HGB value as the HGB value of the blood sample.

[0185] Because the presence of bubbles during the test process will cause the HGB value test result to be biased high, the present application proposes selecting the minimum value from the first HGB value and the second HGB value as the HGB value of the blood sample. This can minimize the impact of bubbles on the HGB value test result and improve the accuracy of the HGB test result.

[0186] If the functions described in the method of this embodiment are implemented in the form of a software functional unit and sold or used as an independent product, they can be stored in a storage medium readable by a computing device. Based on this understanding, the part of the embodiment of the present application that contributes to the prior art or the part of the technical solution can be embodied in the form of a software product, which is stored in a storage medium and includes a number of instructions for enabling a computing device (which can be a personal computer, server, mobile computing device or network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes: various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk.

[0187] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0188] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present application. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application is not limited to the embodiments shown herein, but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for detecting hemoglobin concentration, characterized in that: include: detecting a first test sample injected into the reaction cell and obtaining a first HGB value; The first test sample includes a diluent, a blood sample, and a first hemolytic agent; detecting a second test sample injected into the reaction cell and obtaining a second HGB value; The second test sample is obtained by adding a second hemolytic agent to the first test sample in the reaction pool and mixing; It is determined whether there are bubbles during the detection process. If there are bubbles during the detection process, the minimum value between the first HGB value and the second HGB value is selected as the HGB value of the blood sample.

2. The method according to claim 1, wherein The detecting of the first test sample injected into the reaction cell and obtaining a first HGB value, and the detecting of the second test sample injected into the reaction cell and obtaining a second HGB value include: Detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

3. The method according to claim 2, wherein The method comprises detecting the dilution solution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; Calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes: detecting the dilution solution injected into the reaction cell and obtaining the background voltage; After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining a first sample voltage, and calculating the first HGB value based on the background voltage and the first sample voltage; A second test sample injected into the reaction cell is detected to obtain a second sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

4. The method according to claim 2, wherein The method comprises detecting the dilution solution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; Calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes: detecting a first test sample injected into the reaction cell and obtaining a first sample voltage; detecting a second test sample injected into the reaction cell and obtaining a voltage of the second sample; After draining the reaction cell, detecting the dilution solution injected into the reaction cell and obtaining the background voltage; The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

5. The method according to claim 2, wherein The method comprises detecting the dilution solution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; Calculating a first HGB value based on the background voltage and the first sample voltage, and calculating a second HGB value based on the background voltage and the second sample voltage, includes: detecting the dilution solution injected into the reaction cell and obtaining a first background voltage; After draining the reaction cell, detecting a first test sample injected into the reaction cell and obtaining the first sample voltage; detecting a second test sample injected into the reaction cell and obtaining a voltage of the second sample; After draining the reaction cell, re-detecting the dilution solution injected into the reaction cell and obtaining a second background voltage; selecting the larger of the first background voltage and the second background voltage as the background voltage; The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

6. The method according to any one of claims 2 to 5, characterized in that The calculating the first HGB value based on the background voltage and the first sample voltage includes: According to the formula Calculating and obtaining the first HGB value; The calculating the second HGB value based on the background voltage and the second sample voltage includes: According to the formula Calculating and obtaining the second HGB value; Wherein, the first parameter K1 and the second parameter K2 are both predetermined constants; The first parameter K1 is determined by the first hemolysis dose, the diluent volume and the blood sample volume; The second parameter K2 is determined by the first hemolytic dose, the second hemolytic dose, the diluent volume, and the blood sample volume.

7. The method according to claim 1, wherein The determination of whether there are bubbles during the detection process includes: determining whether an absolute value of a difference between the second HGB value and the first HGB value is greater than a first preset threshold, if so, determining that bubbles are present during the detection process; otherwise, determining that no bubbles are present during the detection process; or, It is determined whether the ratio of the second HGB value to the first HGB value is greater than a second preset threshold value. If so, it is determined that there are bubbles during the detection process; otherwise, it is determined that there are no bubbles during the detection process.

8. The method according to claim 1, wherein Also includes: The first test sample injected into the reaction pool is tested and white blood cell classification data is obtained.

9. The method according to claim 1, wherein Also includes: The second test sample injected into the reaction pool is detected and white blood cell count data is obtained.

10. The method according to claim 6, wherein A first parameter K1 for calculating the first HGB value and a second parameter K2 for calculating the second HGB value are stored in advance.

11. The method according to claim 1, wherein The hemoglobin concentration detection method is applied to an animal-type blood cell analyzer.

12. A blood cell analyzer, characterized in that: include: reaction tank; a diluent supply device, for providing diluent to the reaction tank; a sample supply device, for providing a blood sample to the reaction cell; a hemolytic agent supply device for supplying a first hemolytic agent to the reaction pool to mix with the blood sample and the diluent to obtain a first test sample, and for supplying a second hemolytic agent to the reaction pool to mix with the first test sample to obtain a second test sample; A mixing device, used to mix the first test sample or the second test sample in the reaction pool respectively; The detection device is used to detect the first test sample in the reaction pool to obtain a first HGB value, and to detect the second test sample in the reaction pool to obtain a second HGB value, determine whether bubbles are present during the detection process, and if bubbles are present during the detection process, select the minimum value between the first HGB value and the second HGB value as the HGB value of the blood sample.

13. The blood cell analyzer according to claim 12, wherein: The detection device includes an HGB detection module for detecting an HGB value, wherein the HGB detection module is used to: Detecting the dilution injected into the reaction cell and obtaining a background voltage, detecting the first test sample injected into the reaction cell and obtaining a first sample voltage, and detecting the second test sample injected into the reaction cell and obtaining a second sample voltage; The first HGB value is calculated based on the background voltage and the first sample voltage, and the second HGB value is calculated based on the background voltage and the second sample voltage.

14. The blood cell analyzer according to claim 12 or 13, wherein: The detection device further includes a white blood cell detection module for detecting white blood cells in the detection sample. The white blood cell detection module measures optical information of the first detection sample to count white blood cells in the blood sample.

15. The blood cell analyzer according to claim 14, wherein: The white blood cell detection module is further used to measure the optical information of the second detection sample to perform white blood cell classification on the blood sample.

16. The blood cell analyzer according to claim 12, wherein: The blood cell analyzer is applicable to an animal-type blood cell analyzer.

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