Kelp RNA extraction device and application method

Abstract

The present invention provides a kelp RNA extraction device and an application method, comprising a bracket (100) fixed to a frame; a test tube (200) for containing an extracting solution; a stirring head (300) for stirring the extracting solution in the test tube (200); a driving unit (400) for driving a swash plate mechanism to move via a motor (401) so as to cause the stirring head (300) to reciprocate, thereby fully mixing and stirring the extracting solution in the test tube (200); the stirring head (300) is driven to reciprocate via the driving unit (400), thereby completing the mixing and stirring of the extracting solution in the test tube (200) with a relatively uniform and gentle force, thereby improving experimental efficiency and facilitating the acquisition of high-quality, high-purity, and well-integrity RNA.

Description

Technical Field

[0001] The invention relates to the field of biological applications, in particular to a kelp RNA extraction device capable of fully mixing and stirring an experimental extract instead of manpower. Background Art

[0002] RNA, or ribonucleic acid, is a genetic information carrier that exists in biological cells and some viruses and viroids. Its main function in the body is to guide protein synthesis.

[0003] Kelp is one of my country's most important economic seaweeds, rich in iodine, protein, and various essential minerals. In addition to its edible and medicinal properties, the alginic acid and mannitol it produces are highly valuable industrial raw materials. In recent years, with the rapid development of molecular biology, molecular biology techniques have been applied to kelp genetics, germplasm identification, and phylogenetic research. A prerequisite for molecular biology research is the preparation of high-purity, high-integrity kelp RNA. Molecular biology studies such as Northern blot analysis, purification of mRNA for in vitro translation or cDNA library construction, RT-PCR, and differential analysis all require high-quality, pure, and high-integrity RNA.

[0004] Kelp megaspores contain a high concentration of fucoidan and other components. The TRIzol method for RNA extraction requires thorough homogenization, mixing, centrifugation, and other mechanical steps. Currently, this is mostly done manually by the experimenter, which is time-consuming and labor-intensive. Furthermore, uneven force applied to the test tubes and other human factors can lead to insufficient mixing or stirring of the experimental extract, resulting in poor quality, purity, and integrity of the extracted RNA. Therefore, there is an urgent need for a kelp RNA extraction device that can fully mix and stir the experimental extract, replacing manual labor. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a kelp RNA extraction device which can fully mix and stir the experimental extract instead of manpower.

[0006] The present invention provides a kelp RNA extraction device, comprising

[0007] a bracket, which is fixed to the frame;

[0008] Test tubes, which are used to hold the extract;

[0009] a stirring head, used for stirring the extract in the test tube;

[0010] The driving part drives the swash plate mechanism to move through the motor, so as to make the stirring head move back and forth, so as to fully mix and stir the extract in the test tube.

[0011] The difference between the kelp RNA extraction device and application method of the present invention and the prior art is that the kelp RNA extraction device and application method of the present invention can fully mix and stir the experimental extract instead of manual labor, so as to extract high-quality, high-purity and good-integrity kelp RNA.

[0012] The following further describes the kelp RNA extraction device and application method of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is an axonometric view of the kelp RNA extraction device;

[0014] Figure 2 yes Figure 1 The main view;

[0015] Figure 3 is a partial cross-sectional view of a kelp RNA extraction device;

[0016] Figure 4 It is a partial enlarged view of the swinging part;

[0017] Figure 5 yes Figure 2 Structural deformation diagram;

[0018] Figure 6 It is a structural diagram of the spring clamp;

[0019] Figure 7 It is a partial enlarged view of the rotating part;

[0020] Figure 8 It is a partial enlarged view of the connection part;

[0021] Figure 9 yes Figure 8 The motion state change diagram;

[0022] Figure 10 yes Figure 8 Side view of

[0023] Figure 11 yes Figure 8 Structural deformation diagram;

[0024] Figure 12 This is a diagram of the connection of the knock head;

[0025] Figure 13 It is a diagram of a test tube.

[0026] 100-bracket; 200-test tube; 300-stirring head; 400-driving part; 401-motor; 402-first shaft; 403-swash plate; 404-first sleeve; 405-ball head; 406-ball seat; 407-guide rod; 408-guide cylinder; 409-fixing frame; 410-first bevel gear; 412-second bevel gear; 413-support plate; 414-first spring; 415-connecting bearing; 421-second shaft; 422-first pulley; 423-second pulley; 424-belt; 425-third shaft; 500-swinging part, 501-shift lever; 502-block; 503-second spring; 504-first through hole; 505-fixed Fixed plate; 506-first groove; 507-first disc; 508-third spring; 509-first latch; 510-first gear; 511-first ring gear; 512-second sleeve; 601-second gear; 602-second ring gear; 603-third sleeve; 604-annular magnet; 605-guide block; 606-guide rail; 611-spring clamp; 621-connecting spring; 622-friction block; 700-connecting part; 701-fourth sleeve; 702-second through hole; 703-third latch; 704-first slot; 705-second slot; 706-fourth spring; 801-connecting rod; 802-fifth spring; 803-striking head. DETAILED DESCRIPTION

[0027] like Figures 1 to 13 As shown, see Figure 1 、 2 The present invention provides a kelp RNA extraction device and application method, including:

[0028] a bracket 100 , which is fixed to the frame;

[0029] a test tube 200 for containing an extract;

[0030] A stirring head 300, which is used to stir the extract in the test tube 200;

[0031] The driving unit 400 drives the swash plate mechanism to move via the motor 401 , so as to make the stirring head 300 reciprocate to fully mix and stir the extract in the test tube 200 .

[0032] In the present invention, the driving unit 400 drives the stirring head 300 to reciprocate, thereby mixing and stirring the extract in the test tube 200 with relatively uniform and gentle force, thereby improving experimental efficiency and facilitating the acquisition of high-quality, high-purity, and well-integrity RNA.

[0033] Among them, see Figure 2 The bottom of the test tube 200 overlaps with the bracket 100 and allows the test tube 200 to rotate.

[0034] The present invention achieves a supporting effect on the test tube 200 through the above arrangement.

[0035] The swash plate mechanism includes a swash plate 403 , a support plate 413 , a ball head 405 , a ball seat 406 , and a guide rod 407 .

[0036] For further explanation of the present invention, see Figure 1 、 2 3. The driving unit 400 includes a motor 401, a first shaft 402, a swash plate 403, a first sleeve 404, a ball head 405, a ball seat 406, a guide rod 407, a guide cylinder 408, a fixing frame 409, a first helical gear 410, a second helical gear 412, a support plate 413, a first spring 414, and a connecting bearing 415;

[0037] A motor 401 is fixed to the bracket 100. The output shaft of the motor 401 is drivingly connected to a first shaft 402. The first shaft 402 is fixed to the center of the upper surface of a swash plate 403. The first shaft 402 is bearing-connected to a first sleeve 404. The swash plate 403 is disposed within the first sleeve 404. The first sleeve 404 is fixed to the bracket 100.

[0038] The lower surface of the swash plate 403 is in contact with the upper surface of the support plate 413. The lower surface of the support plate 413 is fixed to the ball head 405. The ball head 405 is disposed in and rotates along the ball seat 406. The ball seat 406 is fixed to one end of a guide rod 407. The other end of the guide rod 407 is fixed to the stirring head 300. The guide rod 407 is disposed in and moves and rotates along the guide cylinder 408. The guide cylinder 408 is fixed to a fixing frame 409. The fixing frame 409 is fixed to the bracket 100.

[0039] The outer circumference of the guide rod 407 is fixed to one end of the first spring 414, the other end of the first spring 414 is fixed to the inner ring of the connecting bearing 415, and the outer ring of the connecting bearing 415 is coaxially fixed to the guide cylinder 408;

[0040] A first helical gear 410 is fixed at the center of the lower surface of the swash plate 403 . The first helical gear 410 is meshed with a second helical gear 412 . The second helical gear 412 is coaxially fixed to the guide rod 407 .

[0041] The first structure consisting of the ball head 405 , ball seat 406 , guide rod 407 , guide cylinder 408 , second bevel gear 412 , support plate 413 , first spring 414 , and connecting bearing 415 is evenly arranged in four groups around the circumference of the first shaft 402 .

[0042] In the present invention, the motor 401 drives the first shaft 402 to rotate, thereby driving the swash plate 403 to rotate. The swash plate 403 reciprocates along the inner circumference of the first sleeve 404, driving the guide rod 407 and the stirring head 300 to alternately reciprocate up and down along the guide cylinder 408. The swash plate 403 drives the first bevel gear 410 to rotate, driving the second bevel gear 412 to rotate forward, reverse, or in a variable-acceleration unidirectional manner. The second bevel gear 412 reciprocates up and down with the guide rod 407, driving the guide rod 407 to rotate with the second bevel gear 412 at a variable speed. This enables the stirring heads 300 in the four groups of the first structure to alternately reciprocate up and down with relatively uniform and gentle force while rotating at a variable speed, thereby improving the efficiency of mixing and stirring the extract in the test tube 200, thereby facilitating the acquisition of high-quality, high-purity, and high-integrity RNA.

[0043] The output shaft of the motor 401 is coaxially fixed with the first shaft 402 .

[0044] The present invention realizes direct driving of the first shaft 402 by the motor 401 through the above arrangement.

[0045] The number of the first structures is at least 2, preferably 4.

[0046] Through the above arrangement, the present invention can significantly improve the mixing and stirring efficiency while reducing the number of structural parts, thereby achieving lightweight equipment structure.

[0047] The guide cylinder 408 in the first structure is fixed to the fixing frame 409 .

[0048] The present invention achieves the support and fixation of the four groups of guide cylinders 408 through the above arrangement.

[0049] For further explanation of the present invention, see Figure 1 、 2 3. The power module provided in the motor 401 includes a battery, an electric control module, and a wireless communication module. The wireless communication module is electrically connected to the electric control module, and the wireless communication module is wirelessly connected to the user terminal.

[0050] The present invention can directly enable the user terminal to wirelessly control the motor 401 through the wireless communication module, so that the speed, rotation direction and power of the motor 401 can be adjusted, thereby arbitrarily adjusting the reciprocating speed of the stirring head 300.

[0051] For further explanation of the present invention, see Figure 1 、 2 3. The motor 401 is a reduction motor.

[0052] Through the above arrangement, the present invention achieves a gentler mixing and stirring of the extract in the test tube 200, thereby avoiding damage to the RNA in the extract.

[0053] For further explanation of the present invention, see Figure 1 、 2 3. The first sleeve 404 is provided with a through hole, and the first shaft 402 is connected to the through hole bearing of the first sleeve 404.

[0054] The present invention achieves support and fixation of the first shaft 402 through the above arrangement.

[0055] For further explanation of the present invention, see Figure 1 、 2 3. The outer circumferential surface of the swash plate 403 is in contact with the inner circumferential surface of the first sleeve 404 and moves along it.

[0056] The present invention achieves a guiding effect on the outer circumferential surface of the swash plate 403 through the above arrangement.

[0057] For further explanation of the present invention, see Figure 1 、 2 3. The support plate 413 is fixed to the connecting block, and the connecting block is fixed to the ball head 405. The diameter of the support plate 413 is greater than the length of the connecting block.

[0058] The present invention avoids failure of the connection between the support plate 413 and the swash plate 403 through the above arrangement, achieves continuous connection and transmission between the support plate 413 and the swash plate 403, and avoids motion interference between components.

[0059] For further explanation of the present invention, see Figure 1 、 2 , 3, 4, the driving unit 400 further includes a second shaft 421, a first pulley 422, a second pulley 423, and a belt 424;

[0060] The bracket 100 is provided with a swinging part 500, which includes a lever 501, a block 502, a second spring 503, a first through hole 504, a fixing plate 505, a first groove 506, a first disc 507, a third spring 508, a first bayonet 509, a first gear 510, a first gear ring 511, and a second sleeve 512;

[0061] The output shaft of the motor 401 is drivingly connected to the second shaft 421, the second shaft 421 is coaxially fixed to the first pulley 422, the first pulley 422 is connected to the second pulley 423 via a belt 424, and the second pulley 423 is coaxially fixed to the first shaft 402;

[0062] The second shaft 421 is fixed to one end of the lever 501. The other end of the lever 501 can overlap one end of the clamping block 502. The other end of the clamping block 502 is fixed to one end of the second spring 503. The other end of the second spring 503 is fixed to the end of the first through hole 504. The clamping block 502 is disposed in the first through hole 504 and moves along the first through hole 504. The first through hole 504 is formed on a fixed plate 505, and the fixed plate 505 is fixed to the bracket 100.

[0063] One end of the clamping block 502 can be disposed in a first groove 506. The first groove 506 is formed on the outer circumference of a first disc 507. The first disc 507 is connected to the second shaft 421 by a bearing. The first disc 507 is coaxially arranged with the fixed disc 505. The first disc 507 is fixed to one end of a third spring 508. The other end of the third spring 508 is fixed to the lever 501. The end surface of the lever 501 can overlap with a first bayonet 509. The first bayonet 509 is fixed to the first disc 507.

[0064] The second structure consisting of the clamping block 502, the second spring 503, and the first through hole 504 is evenly arranged in four groups along the circumference of the fixed disk 505;

[0065] The first disc 507 is coaxially fixed to the second sleeve 512 , the second sleeve 512 is coaxially fixed to the first gear 510 , the first gear 510 is meshed with the first ring gear 511 , and the first ring gear 511 is connected to the outer circumference of the test tube 200 .

[0066] In the present invention, the motor 401 drives the second shaft 421 to rotate, and the first shaft 402 is driven to rotate through a belt transmission connection composed of the first pulley 422, the second pulley 423, and the belt 424, thereby driving the driving part 400 to move. The second shaft 421 drives the lever 501 to rotate, driving the lever 501 to push the block 502 into the first through-hole 504, thereby releasing the restriction on the movement of the first disc 507 and achieving intermittent rotation of the first disc 507 with each rotation angle of 1 / 4 of a circle. The first gear 510 engages with the first ring gear 511, driving the first ring gear 511 and the test tube 200 to intermittently rotate with each rotation angle of 1 / 4 of a circle, achieving reciprocating motion of the test tube 200 from stationary to rotating and then to stationary, thereby achieving the effect of rotating and oscillating the test tube 200 with relatively uniform and gentle force. The movement of the driving unit 400 cooperates with the reciprocating motion of the stirring head 300, thereby improving the efficiency of mixing and stirring the extract in the test tube 200, thereby facilitating the acquisition of high-quality, high-purity, and high-integrity RNA.

[0067] The output shaft of the motor 401 is coaxially fixed with the first shaft 402 .

[0068] The present invention realizes direct driving of the first shaft 402 by the motor 401 through the above arrangement.

[0069] The belt 424 is a V-belt, a round belt or a flat belt, and the outer circumferential surfaces of the first pulley 422 and the second pulley 423 are provided with grooves that match the cross-sectional shape of the belt 424 .

[0070] The present invention avoids failure of the belt transmission connection composed of the first pulley 422, the second pulley 423, and the belt 424 through the above-mentioned arrangement, thereby achieving more stable connection and transmission.

[0071] Among them, see Figure 4 The shape of the first groove 506 is the same as the cross-sectional shape of the end portion of one end of the block 502 .

[0072] Through the above arrangement, the present invention enables one end of the clamping block 502 to be disposed in the first groove 506 , thereby enabling the clamping block 502 to restrict the movement of the first disc 507 .

[0073] Among them, participants Figure 4 The other end of the lever 501 is an inclined surface. When the lever 501 is rotated to the rightmost end, the inclined surface tilts from top to bottom and from right to left. One end of the clamping block 502 is an inclined surface. The inclined surface of the rightmost clamping block 502 tilts from top to bottom and from left to right.

[0074] Through the above-mentioned arrangement, the present invention can limit the rotation of the first disc 507 when the block 502 is located in the first groove 506, and at the same time, the end face of the other end of the lever 501 can move the end face of one end of the block 502, so that the block 502 moves along the first through hole 504 to release the restriction of the block 502 on the first disc 507.

[0075] The second spring 503 is in a compressed state.

[0076] Through the above arrangement, the present invention realizes that the movement of the first through hole 504 of the clamping block 502 can be reset, so as to realize the restriction effect of the clamping block 502 on the first disc 507 .

[0077] The third spring 508 is in a stretched state.

[0078] Through the above arrangement, the present invention realizes that the third spring 508 can pull the lever 501 to provide the first disc 507 with a rotational stroke after the restriction of the block 502 is released, and at the same time pull the lever 501 to reset.

[0079] The first gear ring 511 is coaxially fixed to the outer circumferential surface of the test tube 200 .

[0080] Through the above arrangement, the present invention enables the test tube 200 to rotate synchronously with the first gear ring 511 .

[0081] The second sleeve 512 is sleeved on the outer circumferential surface of the second shaft 421 , and the second shaft 421 and the second sleeve 512 are clearance-fitted.

[0082] The present invention realizes transmission between the first disc 507 and the first gear 510 through the above arrangement.

[0083] For further explanation of the present invention, see Figure 1 、 3 , 4, 5, the driving unit 400 further includes a second shaft 421, a first pulley 422, a second pulley 423, a belt 424, and a third shaft 425;

[0084] The bracket 100 is provided with a swinging part 500, which includes a lever 501, a block 502, a second spring 503, a first through hole 504, a fixing plate 505, a first groove 506, a first disc 507, a third spring 508, a first bayonet 509, a first gear 510, a first gear ring 511, and a second sleeve 512;

[0085] The output shaft of the motor 401 is drivingly connected to the third shaft 425. The third shaft 425 is fixed to one end of the lever 501. The other end of the lever 501 can overlap one end of the clamping block 502. The other end of the clamping block 502 is fixed to one end of the second spring 503. The other end of the second spring 503 is fixed to the end of the first through hole 504. The first through hole 504 is provided with the clamping block 502 that moves along the first through hole 504. The first through hole 504 is formed on a fixed plate 505, and the fixed plate 505 is fixed to the bracket 100.

[0086] One end of the clamping block 502 can be disposed in the first groove 506. The first groove 506 is provided on the outer circumference of the first disc 507. The first disc 507 is connected to the third shaft 425 by a bearing. The first disc 507 is coaxially fixed to the second shaft 421. The second shaft 421 is coaxially fixed to the first pulley 422. The first pulley 422 is connected to the second pulley 423 via a belt drive 424. The second pulley 423 is coaxially fixed to the first shaft 402.

[0087] The first disc 507 is coaxially arranged with the fixed disc 505. The first disc 507 is fixed to one end of a third spring 508. The other end of the third spring 508 is fixed to the lever 501. The end surface of the lever 501 can overlap with the first bayonet 509. The first bayonet 509 is fixed to the first disc 507.

[0088] The second structure consisting of the clamping block 502, the second spring 503, and the first through hole 504 is evenly arranged in four groups along the circumference of the fixed disk 505;

[0089] The first disc 507 is coaxially fixed to the second sleeve 512 , the second sleeve 512 is coaxially fixed to the first gear 510 , the first gear 510 is meshed with the first ring gear 511 , and the first ring gear 511 is connected to the outer circumference of the test tube 200 .

[0090] The present invention drives the first shaft 402 to rotate through a belt transmission connection composed of the first pulley 422 , the second pulley 423 , and the belt 424 , thereby driving the driving part 400 to move.

[0091] In the present invention, the motor 401 drives the third shaft 425 to rotate, drives the shift lever 501 to rotate, and drives the shift lever 501 to shift the block 502 into the first through hole 504, so as to release the restriction on the movement of the first disc 507, and realize the intermittent rotation of the first disc 507 with an angle of 1 / 4 of a circle each time. The first gear 510 is engaged with the first gear ring 511, and the first gear ring 511 and the test tube 200 are driven to intermittently rotate, and the angle of the test tube 200 is 1 / 4 of a circle each time, so as to realize the reciprocating motion of the test tube 200 from static to rotating and then to stopping, thereby achieving the following The relatively uniform and gentle force of the rotational oscillation of the test tube 200 causes the first disc 507 to drive the second shaft 421 to rotate, driving the belt drive connection consisting of the first pulley 422, the second pulley 423, and the belt 424, driving the first shaft 402 to rotate synchronously with the first disc 507, i.e., a reciprocating motion from stationary to rotating and then to stationary. This in turn drives the drive unit 400 to move, driving the stirring head 300 to reciprocate from stationary to rotating and then to stationary, thereby jointly improving the mixing and stirring efficiency of the extract in the test tube 200, thereby facilitating the acquisition of high-quality, high-purity, and high-integrity RNA.

[0092] The output shaft of the motor 401 is coaxially fixed with the third shaft 425 .

[0093] The present invention realizes direct driving of the third shaft 425 by the motor 401 through the above arrangement.

[0094] For further explanation of the present invention, see Figure 1 、 2 , 3, 4, 6, 7, 12, the bracket 100 is provided with a rotating portion, the rotating portion including a second gear 601, a second ring gear 602, a third sleeve 603, an annular magnet 604, a guide block 605, and a guide rail 606;

[0095] The output shaft of the motor 401 is drivingly connected to the second gear 601, which is capable of meshing with the second ring gear 602. The second ring gear 602 and the first ring gear 511 are coaxially fixed to the upper and lower ends of the outer circumferential surface of the third sleeve 603, respectively. The third sleeve 603 is configured to be arranged outside the outer circumferential surface of the test tube 200 via a key connection and moves and rotates synchronously therewith. An annular magnet 604 is fixed to the outer circumferential surface of the test tube 200, and the annular magnet 604 can be attracted to or repelled by the first ring gear 511.

[0096] The outer circumferential surface of the third sleeve 603 is connected to the guide block 605 with a bearing. The guide block 605 is arranged in a guide rail 606 and moves along it. The guide rail 606 is provided on the bracket 100 .

[0097] The present invention controls the end face of the first gear ring 511 to be attracted and / or repelled by the annular magnet 604 by controlling the power supply state of the annular magnet 604. When the end face of the first gear ring 511 can be attracted by the annular magnet 604, the second gear 601 engages with the second gear ring 602 for transmission, the first gear 510 disengages from the first gear ring 511, and the test tube 200 performs a centrifugal action of uniform rotation along with the second gear ring 602. When the end face of the first gear ring 511 repels the annular magnet 604, the first gear 510 engages with the first gear ring 511, the second gear 601 disengages from the second gear ring 602, and the test tube 200 performs a reciprocating motion from stationary to rotating and then to stationary along with the first gear ring 511, thereby achieving the effect of rotating and oscillating the test tube 200 with a relatively uniform and gentle force, and realizing free control and switching of mixing and stirring of the extract in the test tube 200, rotational oscillation action, and centrifugal action, thereby facilitating the operation of the experimenter and improving the experimental efficiency.

[0098] Among them, see Figure 2 、 13 The test tube 200 is a special-shaped test tube, and the bottom of the test tube 200 is tilted away from its axis.

[0099] The present invention adopts the above arrangement to fully centrifuge the extract in the deflected test tube 200 with a relatively uniform and gentle force, while ensuring the requirement of eccentric centrifugation.

[0100] The bottom of the test tube 200 can overlap with the bracket, and the test tube 200 can rotate.

[0101] The present invention achieves a supporting effect on the test tube 200 through the above arrangement.

[0102] The output shaft of the motor 401 is coaxially fixed with the second gear 601 .

[0103] The present invention realizes direct driving of the second gear 601 by the motor 401 through the above arrangement.

[0104] The third sleeve 603 and the test tube 200 are connected by a key as follows: a long key is fixed on the outer circumference of the test tube 200 , the long key is arranged in a key slot, and the key slot is provided on the inner circumference of the third sleeve 603 .

[0105] Through the above arrangement, the present invention realizes that the third sleeve 603 , the first gear ring 511 , and the second gear ring 602 drive the test tube 200 to rotate while the test tube 200 moves along the length direction of the test tube 200 under the action of the annular magnet 604 .

[0106] Of course, see Figure 6 , the inner circumference of the third sleeve 603 is evenly fixed with multiple groups of spring clamps 611, the spring clamps 611 include a connecting spring 621 and a friction block 622, one end of the connecting spring 621 is fixed to the inner circumference of the third sleeve 603, and the other end of the connecting spring 621 is fixed to the friction block 622, and the friction block 622 can overlap with the outer circumference of the test tube 200. The spring clamps 611 can make the third sleeve 603 and the test tube rotate synchronously, and at the same time facilitate the disassembly of the test tube 200. At this time, the third sleeve 603 and the test tube 200 will move synchronously under the action of the annular magnet 604. It is a prior art and will not be described here.

[0107] The distance between the first gear ring 511 and the annular magnet 604 is equal to the distance between the second gear ring 602 and the second gear 601 .

[0108] Through the above arrangement, the present invention realizes that when the second gear 601 is engaged with the second gear ring 602 for transmission, the first gear 510 is disengaged from the first gear ring 511, and when the first gear 510 is engaged with the first gear ring 511, the second gear 601 is disengaged from the second gear ring 602.

[0109] Among them, the annular magnet 604 is an electromagnet, the first gear ring 511 is a permanent magnet, the power module provided by the electromagnet includes a battery, an electronic control module, and a wireless communication module, the wireless communication module is electrically connected to the electronic control module, and the wireless communication module is wirelessly connected to the user terminal.

[0110] The present invention can directly enable the user terminal to wirelessly control the annular magnet 604 through the wireless communication module, so that the user can freely control the engagement of the first gear 510 with the first ring gear 511 or the engagement of the second gear 601 with the second ring gear 602 .

[0111] Those skilled in the art can adjust the magnetic control accuracy of the annular magnet 604 as needed to freely control the distance that the third sleeve 603 and the first gear ring 511 and the second gear ring 602 move along the length direction of the test tube 200. This is existing technology and will not be described in detail here.

[0112] For further explanation of the present invention, see Figure 3 、 8 , 9, 10, the output shaft of the motor 401 is provided with a connecting portion 700, the connecting portion 700 includes a fourth sleeve 701, a second through hole 702, a third bayonet 703, a first bayonet groove 704, a second bayonet groove 705, and a fourth spring 706;

[0113] The output shaft of the motor 401 is coaxially fixed to the fourth sleeve 701 . The second shaft 421 is mounted on the inner circumference of the fourth sleeve 701 via a bearing. The second gear 601 is mounted on the outer circumference of the fourth sleeve 701 via a bearing.

[0114] A second through hole 702 is formed on the side surface of the fourth sleeve 701. A third latch 703 is disposed in the second through hole 702 and moves along the third latch. One end of the third latch 703 can be disposed in and move along the first slot 704, and the other end of the third latch 703 can be disposed in and move along the second slot 705. The third latch 703 is fixed to one end of the fourth spring 706, and the other end of the fourth spring 706 is fixed to the fourth sleeve 701.

[0115] The first clamping groove 704 is formed on the second shaft 421 , and the second clamping groove 705 is formed on the inner circumferential surface of the second gear 601 .

[0116] The present invention drives the fourth sleeve 701 to rotate through the motor 401, and controls the rotation speed of the output shaft of the motor 401 to control the third pin 703 to be located in the first slot 704 or the second slot 705, so as to control the rotation of the first shaft 402 or the second gear 601 according to the different rotation speeds of the output shaft of the motor 401. When the rotation speed of the motor 401 is low, the second shaft 421 is driven to rotate, thereby realizing the movement of the driving part 400 and the swinging part 500, driving the test tube 200 to mix and stir the extract therein and perform a rotational oscillation action. When the rotation speed of the motor 401 is high, the second gear 601 is driven to rotate, thereby realizing the movement of the rotating part, driving the test tube 200 to perform a centrifugal action of uniform rotation of the extract therein with a relatively uniform and gentle force.

[0117] The rotation speed of the motor 401 of 1000-2000 r / min is defined as a low rotation speed, and the rotation speed of 2001-4000 r / min is defined as a high rotation speed.

[0118] When using, see Figure 8 When the output shaft of the motor 401 rotates at a low speed, the third latch 703 is located in the first slot 704, and the output shaft of the motor 401 drives the second shaft 421 to rotate, thereby realizing the movement of the driving unit 400 and the swinging unit 500, driving the test tube 200 to mix and stir the extract therein and perform a rotational oscillation action with relatively uniform and gentle force;

[0119] See also Figure 9When the output shaft of the motor 401 rotates at a high speed, the third pin 703 moves along the second through hole 702 into the second slot 705, and the output shaft of the motor 401 drives the second gear 601 to rotate, thereby realizing the movement of the rotating part, driving the test tube 200 to perform a centrifugal action of uniform rotation of the extract therein with a relatively uniform and gentle force.

[0120] The fourth spring 706 is in a compressed state.

[0121] Through the above-mentioned arrangement, the present invention realizes that the fourth spring 706 tends to configure the third pin 703 in the first slot 704, so that when the output shaft speed of the first motor 401 changes from high to low, the third pin 703 can automatically reset and move along the second through hole 702 to the first slot 704, that is, the motor 401 is normally in a low-speed rotation state to drive the second shaft 421 to rotate, thereby realizing the movement of the driving part 400 and the swinging part 500, driving the test tube 200 to mix and stir the extract therein and perform rotational oscillation with a relatively uniform and gentle force.

[0122] For further explanation of the present invention, see Figure 3 、 10 11. A connecting portion 700 is disposed on the output shaft of the motor 401. The connecting portion 700 includes a fourth sleeve 701, a second through hole 702, a third bayonet 703, a first bayonet slot 704, a second bayonet slot 705, and a fourth spring 706.

[0123] The output shaft of the motor 401 is coaxially fixed to the fourth sleeve 701 . The third shaft 425 is mounted on the inner circumference of the fourth sleeve 701 via a bearing, and the second gear 601 is mounted on the outer circumference of the fourth sleeve 701 via a bearing.

[0124] A second through hole 702 is formed on the side surface of the fourth sleeve 701. A third latch 703 is disposed in the second through hole 702 and moves along the third latch. One end of the third latch 703 can be disposed in and move along the first slot 704, and the other end of the third latch 703 can be disposed in and move along the second slot 705. The third latch 703 is fixed to one end of the fourth spring 706, and the other end of the fourth spring 706 is fixed to the fourth sleeve 701.

[0125] The first latching groove 704 is formed on the third shaft 425 , and the second latching groove 705 is formed on the inner circumferential surface of the second gear 601 .

[0126] The present invention drives the fourth sleeve 701 to rotate through the motor 401, and controls the rotation speed of the output shaft of the motor 401 to control the third pin 703 to be located in the first slot 704 or the second slot 705, so as to control the rotation of the third shaft 425 or the second gear 601 according to the different rotation speeds of the output shaft of the motor 401. When the rotation speed of the motor 401 is low, the third shaft 425 is driven to rotate, thereby realizing the movement of the driving part 400 and the swinging part 500, driving the test tube 200 to mix and stir the extract therein and perform a rotational oscillation action. When the rotation speed of the motor 401 is high, the second gear 601 is driven to rotate, thereby realizing the movement of the rotating part, driving the test tube 200 to perform a centrifugal action of uniform rotation of the extract therein with a relatively uniform and gentle force.

[0127] For further explanation of the present invention, see Figure 3 、 12 The block 502 is fixed to one end of the connecting rod 801, the other end of the connecting rod 801 is fixed to one end of the fifth spring 802, and the other end of the fifth spring 802 is fixed to the knocking head 803, and the knocking head 803 can overlap with the outer circumferential surface of the test tube 200.

[0128] Through the above-mentioned arrangement, the present invention can achieve that the knocking head 803 can knock the outer circumferential surface of the test tube 200 while mixing and stirring the extract in the test tube 200 and rotating and oscillating the extract in the test tube 200, thereby accelerating the vibration of the extract in the test tube 200. Since the blocks 502 will enter the first groove 506 one by one, only two adjacent blocks 502 will drive the connecting rod 801 and the knocking head 803 to knock the outer circumferential surface of the test tube 200 at a time, and the two adjacent blocks 502 will gradually rotate and switch.

[0129] The knocking head 803 is made of a soft material such as rubber or PE to prevent the test tube 200 from being broken by knocking too hard. This is a prior art and will not be described in detail here.

[0130] For further explanation of the present invention, see Figure 4 The cross-sectional shape of the block 502 is square or oval, and the cross-sectional shape of the first through hole 504 is square or oval matching the cross-sectional shape of the block 502 .

[0131] The present invention prevents the block 502 from rotating in the first through hole 504 through the above arrangement.

[0132] An application method of a kelp RNA extraction device is characterized by comprising the following steps:

[0133] Step 1: Collect fresh kelp sporophytes from a marine kelp culture area, excise the growing part of the tissue block, scrub to remove surface impurities, cut into 1cm*1cm squares, place in a cryopreservation tube, and quickly immerse in liquid nitrogen for freezing. Quickly transfer the small tube of material frozen in liquid nitrogen to a mortar pre-cooled with liquid nitrogen, grind to a powder, and add it to test tube 200. During this period, liquid nitrogen is continuously added. Add 1ml of TRIzol reagent for every 80-100mg of tissue;

[0134] Step 2: Control the speed of the motor 401 to 1500 rpm, move the stirring head 300 to homogenize the slurry, let it stand at room temperature for 5 minutes, control the annular magnet 604 to engage with the first gear ring 511 to rotate and vibrate the test tube 200, and simultaneously, the knocking head 803 knocks the outer wall of the test tube 200 to fully mix it;

[0135] Step 3: Add chloroform, control the speed of the motor 401 to 1500 rpm, move the stirring head 300, control the annular magnet 604 to engage with the first gear ring 511 to rotate and vibrate the test tube 200, and simultaneously, use the knocking head 803 to knock the outer wall of the test tube 200 for 30 seconds, and let it stand on ice for 3 minutes;

[0136] Step 4: Control the annular magnet 604 to repel the first gear ring 511, control the speed of the motor 401 to 12000 rpm, and centrifuge for 5 minutes;

[0137] Step 5: Take the upper aqueous phase and mix it with isopropyl alcohol in another test tube 200. Control the speed of the motor 401 to 1500 rpm, move the stirring head 300, control the annular magnet 604 and the first ring gear 511 to engage with each other to rotate and vibrate the test tube 200, and simultaneously use the knocking head 803 to knock the outer wall of the test tube 200. Mix for 10 minutes.

[0138] Step 6: Control the annular magnet 604 to repel the first ring gear 511, control the speed of the motor 401 to 12000 rpm, and centrifuge for 10 minutes;

[0139] Step 7: discard the supernatant and add 75% ethanol for washing;

[0140] Step 8: Control the annular magnet 604 to repel the first ring gear 511, control the speed of the motor 401 to 12000 rpm, and perform air centrifugal drying for 10 minutes to remove excess ethanol;

[0141] Step 9: Dissolve in DEPC-H2O and store at -70°C.

[0142] Experimental data

[0143] 10g of kelp sporophyte tissue was divided into two groups. 5g of kelp sporophyte tissue in each group was added to test tube 200 according to step 1. 50ml of TRIzol reagent was also added. RNA was extracted from kelp using different operation methods. The experimental data are as follows:

[0144] Control group:

[0145] This group used manual mixing and shaking of kelp spore tissue and TRIzol reagent, and centrifuged them. After the experiment, 0.81 ml of sample was obtained, the A260 / 280 value was 1.97, and the kelp RNA concentration was 420 ng / μl.

[0146] Experimental group:

[0147] This group followed steps 1 to 9 instead of manual operation to mix, shake, centrifuge, and perform all operations on the kelp sporophyte tissue and TRIzol reagent. After the experiment, 1.03 ml of sample was obtained, the A260 / 280 value was 2.01, and the kelp RNA concentration was 550 ng / μl.

[0148] According to the above experimental data, the following table is made:

[0149]

[0150] The above experimental data show that the experimental group replaced the manual homogenization and mixing operations in the process with the use of the kelp RNA extraction device, and the content of the sample was greatly improved. At the same time, the concentration of the kelp RNA was also greatly improved. This not only saved manpower, material resources and time, and improved operational efficiency, but also achieved sufficient homogenization, mixing and centrifugation of the reagents, which helped to extract high-quality, high-purity and good integrity kelp RNA.

[0151] To prevent RNA degradation during extraction, all reagents, instruments, and equipment used in the experiment were treated with DEPC water and sterilized at high temperatures. All disposable reagents, including test tubes and pipette tips, were RNase-free and specifically designed for RNA extraction.

[0152] The embodiments described above are merely descriptions of preferred implementations of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A kelp RNA extraction device, characterized in that: include A bracket (100) fixed to the frame; a test tube (200) for containing an extract; a stirring head (300) for stirring the extract in the test tube (200); a driving unit (400) which drives the swash plate mechanism to move via a motor (401), thereby causing the stirring head (300) to reciprocate, thereby fully mixing and stirring the extract in the test tube (200); The driving part (400) includes a motor (401), a first shaft (402), a swash plate (403), a first sleeve (404), a ball head (405), a ball seat (406), a guide rod (407), a guide cylinder (408), a fixing frame (409), a first bevel gear (410), a second bevel gear (412), a support plate (413), a first spring (414), and a connecting bearing (415); A motor (401) is fixed on the bracket (100), an output shaft of the motor (401) is drivingly connected to a first shaft (402), the first shaft (402) is fixed to the center of the upper surface of a swash plate (403), the first shaft (402) is bearing-connected to the first sleeve (404), the swash plate (403) is disposed in the first sleeve (404), and the first sleeve (404) is fixed to the bracket (100); The lower surface of the inclined plate (403) is fitted with the upper surface of the support plate (413), the lower surface of the support plate (413) is fixed to the ball head (405), the ball head (405) is arranged in the ball seat (406) and rotates along the ball seat (406), the ball seat (406) is fixed to one end of the guide rod (407), the other end of the guide rod (407) is fixed to the stirring head (300), the guide rod (407) is arranged in the guide cylinder (408) and moves and rotates along the guide cylinder (408), the guide cylinder (408) is fixed to the fixing frame (409), and the fixing frame (409) is fixed to the bracket (100); The outer circumferential surface of the guide rod (407) is fixed to one end of the first spring (414), the other end of the first spring (414) is fixed to the inner ring of the connecting bearing (415), and the outer ring of the connecting bearing (415) is coaxially fixed to the guide cylinder (408); A first helical gear (410) is fixed at the center of the lower surface of the swash plate (403), the first helical gear (410) is meshed with a second helical gear (412), and the second helical gear (412) is coaxially fixed to the guide rod (407); The first structure consisting of the ball head (405), the ball seat (406), the guide rod (407), the guide cylinder (408), the second bevel gear (412), the support plate (413), the first spring (414), and the connecting bearing (415) is evenly arranged in four groups around the circumference of the first axis (402); The driving unit (400) further includes a second shaft (421), a first pulley (422), a second pulley (423), and a belt (424); The bracket (100) is provided with a swinging portion (500), and the swinging portion (500) includes a shifting rod (501), a clamping block (502), a second spring (503), a first through hole (504), a fixing plate (505), a first groove (506), a first circular plate (507), a third spring (508), a first bayonet (509), a first gear (510), a first gear ring (511), and a second sleeve (512); The output shaft of the motor (401) is drivingly connected to the second shaft (421), the second shaft (421) is coaxially fixed to the first pulley (422), the first pulley (422) is connected to the second pulley (423) via a belt (424), and the second pulley (423) is coaxially fixed to the first shaft (402); The second shaft (421) is fixed to one end of the shifting rod (501), the other end of the shifting rod (501) can overlap one end of the clamping block (502), the other end of the clamping block (502) is fixed to one end of the second spring (503), the other end of the second spring (503) is fixed to the end of the first through hole (504), the first through hole (504) is provided with a clamping block (502) that moves along the first through hole, the first through hole (504) is provided on a fixed disk (505), and the fixed disk (505) is fixed to the bracket (100); One end of the clamping block (502) can be arranged in the first groove (506), the first groove (506) is opened on the outer circumferential surface of the first disc (507), the first disc (507) is connected to the second shaft (421) by a bearing, the first disc (507) and the fixed disc (505) are coaxially arranged, the first disc (507) is fixed to one end of the third spring (508), the other end of the third spring (508) is fixed to the shifting rod (501), the end surface of the shifting rod (501) can overlap with the first bayonet (509), and the first bayonet (509) is fixed to the first disc (507); The second structure consisting of the clamping block (502), the second spring (503), and the first through hole (504) is evenly arranged in four groups along the circumference of the fixed disk (505); The first disc (507) is coaxially fixed to the second sleeve (512), the second sleeve (512) is coaxially fixed to the first gear (510), the first gear (510) is meshed with the first ring gear (511), and the first ring gear (511) is connected to the outer circumferential surface of the test tube (200).

2. A kelp RNA extraction device, characterized in that: It includes a bracket (100) fixed to the frame; a test tube (200) for containing an extract; a stirring head (300) for stirring the extract in the test tube (200); a driving unit (400) which drives the swash plate mechanism to move via a motor (401), thereby causing the stirring head (300) to reciprocate, thereby fully mixing and stirring the extract in the test tube (200); The driving part (400) includes a motor (401), a first shaft (402), a swash plate (403), a first sleeve (404), a ball head (405), a ball seat (406), a guide rod (407), a guide cylinder (408), a fixing frame (409), a first bevel gear (410), a second bevel gear (412), a support plate (413), a first spring (414), and a connecting bearing (415); A motor (401) is fixed on the bracket (100), an output shaft of the motor (401) is drivingly connected to a first shaft (402), the first shaft (402) is fixed to the center of the upper surface of a swash plate (403), the first shaft (402) is bearing-connected to the first sleeve (404), the swash plate (403) is disposed in the first sleeve (404), and the first sleeve (404) is fixed to the bracket (100); The lower surface of the inclined plate (403) is fitted with the upper surface of the support plate (413), the lower surface of the support plate (413) is fixed to the ball head (405), the ball head (405) is arranged in the ball seat (406) and rotates along the ball seat (406), the ball seat (406) is fixed to one end of the guide rod (407), the other end of the guide rod (407) is fixed to the stirring head (300), the guide rod (407) is arranged in the guide cylinder (408) and moves and rotates along the guide cylinder (408), the guide cylinder (408) is fixed to the fixing frame (409), and the fixing frame (409) is fixed to the bracket (100); The outer circumferential surface of the guide rod (407) is fixed to one end of the first spring (414), the other end of the first spring (414) is fixed to the inner ring of the connecting bearing (415), and the outer ring of the connecting bearing (415) is coaxially fixed to the guide cylinder (408); A first helical gear (410) is fixed at the center of the lower surface of the swash plate (403), the first helical gear (410) is meshed with a second helical gear (412), and the second helical gear (412) is coaxially fixed to the guide rod (407); The first structure consisting of the ball head (405), the ball seat (406), the guide rod (407), the guide cylinder (408), the second bevel gear (412), the support plate (413), the first spring (414), and the connecting bearing (415) is evenly arranged in four groups around the circumference of the first axis (402); The driving part (400) further includes a second shaft (421), a first pulley (422), a second pulley (423), a belt (424), and a third shaft (425); The bracket (100) is provided with a swinging portion (500), and the swinging portion (500) includes a shifting rod (501), a clamping block (502), a second spring (503), a first through hole (504), a fixing plate (505), a first groove (506), a first circular plate (507), a third spring (508), a first bayonet (509), a first gear (510), a first gear ring (511), and a second sleeve (512); The output shaft of the motor (401) is drivingly connected to the third shaft (425), the third shaft (425) is fixed to one end of the shifting rod (501), the other end of the shifting rod (501) can overlap with one end of the clamping block (502), the other end of the clamping block (502) is fixed to one end of the second spring (503), the other end of the second spring (503) is fixed to the end of the first through hole (504), the first through hole (504) is provided with a clamping block (502) that moves along the first through hole, the first through hole (504) is provided on a fixed disk (505), and the fixed disk (505) is fixed to the bracket (100); One end of the clamping block (502) can be arranged in the first groove (506), the first groove (506) is opened on the outer circumference of the first disc (507), the first disc (507) is connected to the third shaft (425) bearing, the first disc (507) is fixed coaxially with the second shaft (421), the second shaft (421) is fixed coaxially with the first pulley (422), the first pulley (422) is connected to the second pulley (423) through a belt (424), and the second pulley (423) is fixed coaxially with the first shaft (402); The first circular disc (507) and the fixed disc (505) are coaxially arranged, the first circular disc (507) is fixed to one end of a third spring (508), the other end of the third spring (508) is fixed to the shifting rod (501), the end surface of the shifting rod (501) can overlap with the first bayonet (509), and the first bayonet (509) is fixed to the first circular disc (507); The second structure consisting of the clamping block (502), the second spring (503), and the first through hole (504) is evenly arranged in four groups along the circumference of the fixed disk (505); The first disc (507) is coaxially fixed to the second sleeve (512), the second sleeve (512) is coaxially fixed to the first gear (510), the first gear (510) is meshed with the first ring gear (511), and the first ring gear (511) is connected to the outer circumferential surface of the test tube (200).

3. A kelp RNA extraction device according to any one of claims 1 or 2, characterized in that: The bracket (100) is provided with a rotating part, which includes a second gear (601), a second ring gear (602), a third sleeve (603), an annular magnet (604), a guide block (605), and a guide rail (606); The output shaft of the motor (401) is drivingly connected to the second gear (601), the second gear (601) can mesh with the second ring gear (602), the second ring gear (602) and the first ring gear (511) are coaxially fixed to the upper and lower ends of the outer circumferential surface of the third sleeve (603), respectively, the third sleeve (603) is configured outside the outer circumferential surface of the test tube (200) through a key connection and moves and rotates synchronously along the outer circumferential surface, and an annular magnet (604) is fixed on the outer circumferential surface of the test tube (200), and the annular magnet (604) can attract or repel the first ring gear (511); The outer circumferential surface of the third sleeve (603) is connected to the guide block (605) bearing. The guide block (605) is arranged in a guide rail (606) and moves along the guide rail. The guide rail (606) is provided on the bracket (100).

4. A kelp RNA extraction device according to claim 3, characterized in that: The output shaft of the motor (401) is provided with a connecting portion (700), the connecting portion (700) comprising a fourth sleeve (701), a second through hole (702), a third latch (703), a first latch groove (704), a second latch groove (705), and a fourth spring (706); The output shaft of the motor (401) is coaxially fixed to the fourth sleeve (701); the second shaft (421) is mounted on the inner circumferential surface of the fourth sleeve (701) via a bearing; and the second gear (601) is mounted on the outer circumferential surface of the fourth sleeve (701) via a bearing; A second through hole (702) is provided on the side surface of the fourth sleeve (701), and a third latch (703) is arranged in the second through hole (702) and moves along the third through hole. One end of the third latch (703) can be arranged in the first slot (704) and move along the third through hole. The other end of the third latch (703) can be arranged in the second slot (705) and move along the third through hole. The third latch (703) is fixed to one end of the fourth spring (706), and the other end of the fourth spring (706) is fixed to the fourth sleeve (701). The first clamping groove (704) is provided on the second shaft (421), and the second clamping groove (705) is provided on the inner circumferential surface of the second gear (601).

5. The kelp RNA extraction device according to claim 3, wherein: The output shaft of the motor (401) is provided with a connecting portion (700), the connecting portion (700) comprising a fourth sleeve (701), a second through hole (702), a third latch (703), a first latch groove (704), a second latch groove (705), and a fourth spring (706); The output shaft of the motor (401) is coaxially fixed to the fourth sleeve (701); the third shaft (425) is mounted on the inner circumferential surface of the fourth sleeve (701) via a bearing; and the second gear (601) is mounted on the outer circumferential surface of the fourth sleeve (701) via a bearing; A second through hole (702) is provided on the side surface of the fourth sleeve (701), and a third latch (703) is arranged in the second through hole (702) and moves along the third through hole. One end of the third latch (703) can be arranged in the first slot (704) and move along the third through hole. The other end of the third latch (703) can be arranged in the second slot (705) and move along the third through hole. The third latch (703) is fixed to one end of the fourth spring (706), and the other end of the fourth spring (706) is fixed to the fourth sleeve (701). The first clamping groove (704) is provided on the third shaft (425), and the second clamping groove (705) is provided on the inner circumferential surface of the second gear (601); The motor (401) drives the first shaft (402) to rotate, drives the swash plate (403) to rotate, and drives the swash plate (403) to reciprocate along the inner circumference of the first sleeve (404), drives the guide rod (407) and the stirring head (300) to move up and down alternately and reciprocally along the guide cylinder (408), drives the first bevel gear (410) to rotate through the swash plate (403), drives the second bevel gear (412) to rotate forward and reverse or to rotate in one direction with variable acceleration, and drives the second bevel gear (412) to move up and down with the guide rod (407), drives the guide rod (407) to rotate with the second bevel gear (412) at a variable speed, thereby realizing that the stirring heads 3 (300) in the four groups of first structures move up and down alternately and reciprocate while being rotated at a variable speed.

6. The device for extracting RNA from kelp according to claim 3, wherein: The clamping block (502) is fixed to one end of the connecting rod (801), the other end of the connecting rod (801) is fixed to one end of the fifth spring (802), the other end of the fifth spring (802) is fixed to the knocking head (803), and the knocking head (803) can overlap the outer circumferential surface of the test tube (200).

7. The device for extracting RNA from kelp according to claim 1, wherein: The cross-sectional shape of the clamping block (502) is square or elliptical, and the cross-sectional shape of the first through hole (504) is square or elliptical matching the cross-sectional shape of the clamping block (502).

8. An application method of the kelp RNA extraction device according to claim 6, characterized in that The steps include: Step 1: Collect fresh kelp spores from the kelp culture area at sea, cut the tissue block of the growing part, scrub it to remove surface impurities, cut it into 1cm*1cm squares and place it in a cryopreservation tube, quickly immerse it in liquid nitrogen for freezing, quickly transfer a small tube of material frozen in liquid nitrogen to a mortar pre-cooled with liquid nitrogen, continuously add liquid nitrogen during the process, grind it into powder and add it to the test tube (200), add 1ml TRIzol extraction reagent for every 100mg of tissue; Step 2: Control the motor (401) to rotate at 1500 rpm, move the stirring head (300) to homogenize the slurry, let it stand at room temperature for 5 minutes, control the annular magnet (604) to engage with the first gear ring (511) to rotate and vibrate the test tube (200), and at the same time, knock the knocking head (803) to knock the outer wall of the test tube (200) to fully mix it; Step 3: Add chloroform, control the speed of the motor (401) to 1500 r / min, move the stirring head (300), control the annular magnet (604) and the first gear ring (511) to engage with each other to rotate and vibrate the test tube (200), and at the same time, use the knocking head (803) to knock the outer wall of the test tube (200) to vibrate for 30 seconds, and let it stand on ice for 5 minutes; Step 4: Control the annular magnet (604) to repel the first ring gear (511), control the motor (401) to rotate at 12000 rpm, and centrifuge for 5 minutes; Step 5: Take the upper aqueous phase and mix it with isopropyl alcohol in another test tube (200), control the speed of the motor (401) to 1500 r / min, move the stirring head (300), control the annular magnet (604) and the first gear ring (511) to attract and rotate the test tube (200), and at the same time, knock the outer wall of the test tube (200) with the knocking head (803) to mix for 5 minutes; Step 6: Control the annular magnet (604) to repel the first ring gear (511), control the motor (401) to rotate at 12000 rpm, and centrifuge for 10 minutes; Step 7: discard the supernatant and add 75% ethanol for washing; Step 8: Control the annular magnet (604) to repel the first ring gear (511), control the motor (401) to rotate at 12000 rpm, and centrifuge the air for 10 minutes to remove excess ethanol; Step 9: Dissolve in DEPC-H2O and store at -70°C.

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