A staining method for sample staining and related apparatus

Abstract

Embodiments of the present application provide a staining method and related equipment for sample staining, which are used for intelligently adjusting staining parameters to meet the staining requirements in various scenarios. Embodiments of the present application provide a staining method for sample staining, which is applied to a push film staining device. The push film staining device includes a push film mechanism, a staining mechanism, and a conveying mechanism. The push film mechanism is used to load a to-be-tested sample onto a slide to smear the sample to make a sample smear. The staining mechanism is used to stain the to-be-tested sample on the sample smear. The conveying mechanism is used to convey the sample smear from the push film mechanism to the staining mechanism. The specific operation is as follows: obtaining sample information of the to-be-tested sample; controlling the push film mechanism to prepare a sample smear according to the sample information; controlling the conveying mechanism to convey the sample smear from the push film mechanism to the staining mechanism; and controlling the staining mechanism to stain the to-be-tested sample on the sample smear according to the sample information.

Description

Technical Field

[0001] This application relates to the field of medical devices, and more particularly to a staining method and related equipment for staining samples. Background Technology

[0002] Microscopic examination of blood smears is a fundamental and widely used method of blood examination, particularly valuable for the diagnosis of various hematological diseases. The typical staining procedure is as follows: Figure 1 and Figure 2 As shown. Among them. Figure 1 The diagram shows the methanol fixation and staining procedure. Figure 2 The diagram shows the non-methanol fixation staining procedure. In both staining processes described above, the staining parameters for all sample smears are the same.

[0003] In practical applications, different cells have different internal structures, and the required staining depth also varies. If the same staining parameters are used for all cells, the staining requirements cannot be met. Summary of the Invention

[0004] This application provides a staining method and related equipment for sample staining, which can intelligently adjust staining parameters to meet staining needs in various scenarios.

[0005] In a first aspect, embodiments of this application provide a staining method for sample staining, applied to a slide staining apparatus. The slide staining apparatus includes a slide-pushing mechanism for applying a sample to be tested onto a glass slide to form a sample smear; a staining mechanism for staining the sample to be tested on the sample smear; and a transport mechanism for transporting the sample smear from the slide-pushing mechanism to the staining mechanism. The specific operation is as follows: acquiring sample information of the sample to be tested; controlling the slide-pushing mechanism to prepare a sample smear according to the sample information; controlling the transport mechanism to transport the sample smear from the slide-pushing mechanism to the staining mechanism; and controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.

[0006] Secondly, embodiments of this application provide a staining method for staining samples, applied to a staining apparatus including a staining mechanism, wherein the staining mechanism is used to stain a sample to be tested on a sample smear, and the specific operation is as follows: obtaining sample information of the sample to be tested; controlling the staining mechanism to select staining parameters according to the sample information to stain the sample to be tested on the sample smear.

[0007] Thirdly, embodiments of this application provide a slide-staining apparatus, comprising:

[0008] A slide-pushing mechanism is used to load the sample to be tested onto a glass slide and spread it to prepare a sample smear.

[0009] A staining apparatus for staining samples on the sample smear, the staining apparatus having a special staining mode and a normal staining mode;

[0010] A transport mechanism for transporting the sample smear to the staining facility;

[0011] A control mechanism, communicatively connected to the slide pushing mechanism, the transport mechanism, and the staining mechanism, is configured to: determine the operating mode of the staining mechanism from a special staining mode and a normal staining mode; when the operating mode is determined to be a special staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to a first staining parameter, the first staining parameter being determined according to a preset mapping table, the preset mapping table being used to indicate the correspondence between the sample and the staining parameter; when the operating mode is determined to be a normal staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to conventional staining parameters, the conventional staining parameters being the staining parameters pre-configured by the slide pushing and staining device.

[0012] Fourthly, embodiments of this application provide a sample staining apparatus, characterized in that it comprises:

[0013] A staining mechanism is used to stain samples on a sample smear. The staining mechanism has a special staining mode and a normal staining mode. A control mechanism is communicatively connected to the staining mechanism and configured to: determine the operating mode of the staining mechanism from the special staining mode and the normal staining mode; when the operating mode is determined to be the special staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to a first staining parameter, the first staining parameter being determined according to a preset mapping table, the preset mapping table being used to indicate the correspondence between the sample and the staining parameter; when the operating mode is determined to be the normal staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to conventional staining parameters, the conventional staining parameters being the staining parameters pre-configured by the staining device.

[0014] Fifthly, embodiments of this application provide a sample analysis system, characterized in that the sample analysis system includes:

[0015] A sample analysis device is used to test samples and obtain sample information;

[0016] A slide-staining apparatus includes a slide-pushing mechanism and a staining mechanism, wherein the slide-pushing mechanism is configured to prepare a sample smear, and the staining mechanism is configured to stain the sample to be tested on the sample smear;

[0017] A cell image analysis device is designed for capturing and analyzing sample smears;

[0018] The transport device includes a first transport track, a second transport track, and a third transport track. The first transport track is used to transport samples from the sample analysis device to the slide pushing mechanism; the second transport track is used to transport sample smears from the slide pushing mechanism to the staining mechanism; and the third transport track is used to transport sample smears from the staining mechanism to the cell image analysis device.

[0019] A control mechanism, communicatively connected to the sample analysis device, the slide pushing mechanism, the staining mechanism, the cell image analysis device, and the transport device, and configured to:

[0020] Obtain sample information from the sample analysis device;

[0021] The system controls the first transport track to transport the sample to be tested to the slide pushing mechanism; controls the slide pushing mechanism to prepare a sample smear; and controls the second transport track to deliver the sample smear to the staining mechanism; controls the staining mechanism to select staining parameters based on the sample information to stain the sample to be tested on the sample smear; and controls the third transport track to transport the sample smear from the staining mechanism to the cell image analysis device for imaging and analysis.

[0022] In this embodiment, the sample staining parameters are determined based on the sample information to achieve intelligent adjustment of the staining parameters, thereby meeting the staining needs in various scenarios. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the methanol fixation and staining process;

[0024] Figure 2 This is a schematic diagram of a non-methanol fixation and staining process;

[0025] Figure 3 This is a schematic diagram of the slide-staining apparatus in an embodiment of this application;

[0026] Figure 4 This is another structural schematic diagram of the slide-pushing staining device in the embodiments of this application;

[0027] Figure 5 This is another structural schematic diagram of the slide-pushing staining device in the embodiments of this application;

[0028] Figure 6 This is a sample smear pushing and staining process of the pushing and staining device in the embodiments of this application;

[0029] Figure 7 This is a schematic diagram of an embodiment of a staining method for staining samples in this application.

[0030] Figure 8This is a schematic diagram of another embodiment of the staining method for staining samples in this application;

[0031] Figure 9 This is a schematic diagram of the working process of the slide-pushing and staining apparatus in the embodiments of this application;

[0032] Figure 10 This is another schematic diagram of the working process of the slide-pushing and staining device in the embodiments of this application;

[0033] Figure 11 This is a schematic diagram of the sample analysis system in an embodiment of this application. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application are described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. Those skilled in the art will understand that with the emergence of new application scenarios, the technical solutions provided by the embodiments of this application are also applicable to similar technical problems.

[0035] The terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments described herein can be implemented in a sequence other than that illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or device that includes a series of steps or modules is not necessarily limited to those explicitly listed, but may include other steps or modules not explicitly listed or inherent to such processes, methods, products, or devices. The naming or numbering of steps appearing in this application does not imply that the steps in the method flow must be performed in the chronological / logical order indicated by the naming or numbering. The execution order of named or numbered process steps can be changed according to the desired technical purpose, as long as the same or similar technical effect is achieved. The division of units in this application is a logical division. In practical applications, there may be other division methods. For example, multiple units may be combined or integrated into another system, or some features may be ignored or not executed. In addition, the shown or discussed mutual coupling, direct coupling, or communication connection may be through some interface, and the indirect coupling or communication connection between units may be electrical or other similar forms, none of which are limited in this application. Furthermore, the units or sub-units described as separate components may or may not be physically separated, may or may not be physical units, or may be distributed among multiple circuit units. Some or all of the units can be selected to achieve the purpose of the solution in this application according to actual needs.

[0036] For example, the sample analysis system provided in this application embodiment includes a series of devices, but the sample analysis system provided in this application embodiment is not limited to the devices explicitly described. Similarly, the sample analysis method provided in this application embodiment includes a series of steps, but the sample analysis method provided in this application embodiment is not limited to the steps described. It should be noted that in the following description, the term "embodiment" refers to a subset of all possible embodiments, but it is understood that "embodiment" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0037] This embodiment provides a staining method for sample staining, wherein the sample to be tested includes, but is not limited to, microorganisms, blood, body fluids, and bone marrow fluid. To more clearly illustrate the inventive concept of this application, the following description uses a blood smear as a sample and a smear preparation apparatus for preparing the smear as an example. Before further detailed description of the present invention, the nouns and terms involved in the embodiments of the present invention are explained, and the nouns and terms involved in the embodiments of the present invention are subject to the following interpretations.

[0038] 1. Responding to, used to indicate the conditions or states on which the operation performed depends. When the conditions or states on which it depends are met, one or more operations performed can be performed in real time or with a set delay. Unless otherwise specified, there is no restriction on the order in which the multiple operations are performed.

[0039] 2. Sample smear, which is a substrate for smearing the specimen.

[0040] 3. Staining parameters, including staining time, the ratio of staining solution to buffer solution, and temperature. In this embodiment, the staining time, the ratio of staining solution to buffer solution, and temperature are all adjustable. Taking leukocytes as an example, the longer the staining time of the staining solution and buffer solution mixture, the more leukocytes bind to the dye, and the darker the leukocyte color; the higher the ratio of staining solution to buffer solution, the darker the leukocyte staining, and vice versa.

[0041] The slide-staining apparatus provided in this application embodiment is as follows: Figure 3 As shown, the slide-staining device includes a slide-pushing mechanism 301, which is used to load the sample to be tested onto a glass slide and apply it to prepare a sample smear.

[0042] The staining mechanism 303 is used to stain the sample on the sample smear, and the staining mechanism has a special staining mode and a normal staining mode;

[0043] The transport mechanism 302 is used to transport the sample smear to the staining mechanism 303;

[0044] The control mechanism 304 is communicatively connected to the slide pushing mechanism 301, the staining mechanism 303, and the transport mechanism 302. It is configured to: determine the operating mode of the staining mechanism 303 from a special staining mode and a normal staining mode; when the operating mode is determined to be a special staining mode, control the staining mechanism 303 to stain the sample to be tested on the sample smear according to a first staining parameter, the first staining parameter being determined according to a preset mapping table, the preset mapping table indicating the correspondence between the sample and the staining parameter; when the operating mode is determined to be a normal staining mode, control the staining mechanism 303 to stain the sample to be tested on the sample smear according to conventional staining parameters, the conventional staining parameters being the staining parameters pre-configured by the slide pushing and staining device.

[0045] Please refer to the reference. Figure 4 and Figure 5 The slide-staining device in this embodiment can be used for preparing smears of samples such as blood and body fluids, and for staining sample smears. The slide-staining device includes a sampling mechanism 1 for extracting samples and a slide loading mechanism 2 for moving slides to the working line (i.e., equivalent to...). Figure 3 The transport mechanism 302), the sample loading mechanism 3 for loading the sample onto the glass slide, and the slide pushing mechanism 4 for smoothing the sample on the glass slide (i.e., equivalent to...) Figure 3 The slide pushing mechanism 301, the drying mechanism (not shown in the figure) for drying the sample on the slide, and the staining mechanism 5 (equivalent to) for staining the sample on the slide Figure 3 (Staining mechanism 303 in the middle).

[0046] Please refer to Figures 4 to 6 In one embodiment, when the sampling mechanism 1 extracts a sample, it first mixes the sample and then uses a sampling device (e.g., sampling needle 31) in the sampling mechanism 1 to aspirate the sample. Depending on the sample container, the aspiration can be puncture aspiration (the sample container has a lid, and the sampling device passes through the lid) or open aspiration (the sample container is open, and the sampling device aspirates the sample directly from the opening). In some embodiments, a micro-injection mechanism 6 is also included. The micro-injection mechanism 6 can move the test tube placed by the operator directly toward the sampling device, or the sampling device can also move toward the test tube placed by the operator. In other implementations, the micro-injection mechanism 6 can also move the test tube directly toward the sample loading mechanism 3, or the sample loading mechanism 3 can also move toward the test tube placed by the operator. Blood is directly aspirated by the sample loading mechanism 3 (e.g., a blood-drop needle) and then loaded onto the sample. Since it is not necessary to draw blood through the sampling mechanism 1, the blood sample requirement can be reduced, thereby achieving micro-volume and priority injection. After sampling is completed, the blood is prepared to be dripped onto the glass slide by the sample loading mechanism 3.

[0047] Accordingly, the slide loading mechanism 2 extracts the slide and loads it into the appropriate position to facilitate the blood-dropping operation. For example, Figure 6 As shown, in some embodiments, after the slide extraction operation is completed, operations such as slide left-right detection and slide cleaning can be performed before the slide is loaded. The loaded slide can then have relevant information printed on it, and operations such as slide front-back detection can be performed.

[0048] Please continue reading. Figures 4 to 6 After the blood dropper of the sample application mechanism 3 drips the sample onto the glass slide, a slide-pushing operation is performed. The slide-pushing mechanism 4 then pushes the blood onto the slide to form a blood film. Typically, after the slide-pushing process is complete, the blood film on the slide can be dried to stabilize its shape. Figure 6 As shown, in some embodiments, the slide can be rotated before the blood film is dried to meet certain requirements. In some embodiments, the dried blood smear can also be subjected to drying tests to determine the drying effect of the blood film. In some embodiments, the dried blood smear can also be subjected to blood film unfolding tests to determine whether the blood film has unfolded and whether the unfolding state meets the requirements.

[0049] Please continue. Figures 4 to 6 After the sample smear is pushed out, it can be stained by the staining mechanism 5, wherein the staining mechanism 5 has Figure 3 The function of the dyeing mechanism 303 described herein.

[0050] The above Figures 3 to 6 A slide-pushing staining apparatus for implementing the sample staining method in the embodiments of this application is described. It is understood that a structure combining a single slide-pushing apparatus and a single staining apparatus can also be used to implement the sample staining method in the embodiments of this application. The single staining apparatus can have the function of the staining mechanism 303 described above, which will not be repeated here. The single slide-pushing apparatus also has the function of the slide-pushing mechanism 301 described above, which will not be repeated here.

[0051] Please refer to details. Figure 7 As shown, one embodiment of the staining method for sample staining in this application includes:

[0052] 701, obtain sample information of the sample to be tested.

[0053] The slide staining device receives sample information of the sample to be tested sent by the sample analyzer or directly obtains sample information of the sample to be tested input by the user through the input device.

[0054] In this embodiment, the sample information includes at least one of the component information of the sample to be tested, the sample type of the sample to be tested, the source information of the sample to be tested, and the application scenario information of the sample to be tested. The component information (also referred to as cell detection parameters) of the sample to be tested includes hemoglobin information, white blood cell information, and platelet information. The cell detection parameter information can be used to indicate whether the corresponding cell detection parameter value is abnormal or normal. In this embodiment, abnormal cell detection parameter values ​​mainly indicate that the corresponding cell detection parameter value does not meet the corresponding threshold (e.g., greater than the threshold or outside the threshold range), the cell detection parameter is missing (e.g., the cell detection parameter is empty or the corresponding cell detection parameter value is the initial value), or the corresponding cell detection parameter value directly indicates whether there is an abnormality (e.g., it does not need to be determined by a threshold method), etc., are all considered abnormal cell detection parameter values. Conversely, it indicates that the corresponding cell detection parameter value is normal.

[0055] The sample type is used to indicate whether the sample is blood, body fluid, or bone marrow fluid.

[0056] The source information of the sample to be tested includes the information of the person to be tested corresponding to the sample or the department from which the sample originated. For example, the patient's medical history may show that the patient has leukemia; or the sample may originate from a specific department, such as the test results, which may originate from, but are not limited to, the reproductive medicine department. The specific department can be determined according to medical needs, and this embodiment does not impose any limitations.

[0057] The application scenario information of the sample includes the detection items corresponding to the sample. For example, the sample to be tested may only detect white blood cells or only detect platelets, etc. Specific situations are not limited here.

[0058] 702, control the slide pushing mechanism to prepare sample smears according to the sample information.

[0059] In this embodiment, the slide-pushing staining device can directly control the slide-pushing mechanism to prepare a sample smear for the sample to be tested; or, the slide-pushing staining device can determine the number of sample smears prepared by the slide-pushing mechanism based on the sample information.

[0060] When the slide pushing mechanism prepares a sample smear based on the sample information, the specific operation can be as follows: the slide pushing and staining mechanism determines whether the sample to be tested is a special sample or an abnormal sample based on the sample information. If so, the slide pushing and staining device controls the slide pushing mechanism to prepare at least one sample smear for the sample to be tested; if the sample information indicates that the sample to be tested is a normal sample, the slide pushing and staining device controls the slide pushing mechanism to prepare one sample smear for the sample to be tested.

[0061] In this embodiment, when the values ​​corresponding to each component of the sample to be tested are abnormal, the sample to be tested can be called an abnormal sample; and when special cells (such as special lymphocytes) appear in the sample to be tested, or when the source of the sample to be tested is a person to be tested corresponding to a special case, or when the source department of the sample to be tested is a special department, the sample to be tested is a special sample. It is understood that this is only for illustrative purposes and no specific limitation is made here.

[0062] 703, control the transport mechanism to transport the sample smear from the slide pusher to the staining mechanism.

[0063] In the slide staining apparatus, after the sample smear is prepared, the transport mechanism is controlled to transport the sample smear along the production line to the staining mechanism.

[0064] 704, control the staining mechanism to select staining parameters according to the sample information to stain the sample to be measured on the sample smear.

[0065] When the slide staining device determines that the sample to be tested is a special sample or an abnormal sample based on the sample information, the slide staining device controls the staining mechanism to determine the first staining parameter corresponding to the sample to be tested according to a preset mapping table, and uses the staining parameter to stain the sample to be tested on the sample smear; if the slide staining device determines that the sample to be tested is a normal sample based on the sample information, the slide staining device controls the staining mechanism to stain the sample to be tested on the sample smear according to conventional staining parameters.

[0066] In this embodiment, the preset mapping table is used to indicate the correspondence between samples and staining parameters. In one example, the preset mapping table is shown in Table 1 below:

[0067] Table 1

[0068] Sample Information Staining time The ratio of staining solution to buffer solution body fluid 2 minutes 1：18 High white blood cell count 8 minutes 1：6 Low white blood cell count sample 1 minute 1：18

[0069] The staining time and the ratio of staining solution to buffer solution can be set in advance as needed. The slide staining device automatically selects the required staining parameters based on the sample information. The above staining scheme is just one example; in practice, more levels can be defined, such as setting multiple staining parameters based on different white blood cell count ranges.

[0070] In this embodiment of the application, the smear staining device specifically stains the sample to be tested on the sample smear according to the first staining parameters, including the following possible implementation methods:

[0071] In one possible implementation, the slide pushing and staining device controls the slide pushing mechanism to push out multiple sample smears. The slide pushing and staining device then controls the staining mechanism to stain at least one of the sample smears according to the first staining parameters, while the remaining sample smears are stained using conventional staining parameters. For example, if the slide pushing mechanism pushes out three sample smears, A, B, and C, the slide pushing and staining device selects sample smear A to be stained according to the first staining parameters, while the remaining sample smears B and C are stained using conventional staining parameters.

[0072] In another implementation, after determining the first staining parameters of the sample to be tested, the smear staining device adjusts the first staining parameters within a preset range to obtain a set of staining parameters; then, it controls the staining mechanism to stain any one sample smear according to the first staining parameters, and controls the staining mechanism to stain the remaining sample smears according to each set of staining parameters in the set of staining parameters. For example, the first staining parameters of the sample to be tested are: staining time of 2 minutes, staining solution to buffer ratio of 1:18, and staining solution to buffer temperature of 30 degrees Celsius. In an exemplary scheme, the set of staining parameters includes: staining parameter A (staining time of 2 minutes, staining solution to buffer ratio of 1:16, and staining solution to buffer temperature of 32 degrees Celsius), staining parameter B (staining time of 1 minute, staining solution to buffer ratio of 1:16, and staining solution to buffer temperature of 31 degrees Celsius), and staining parameter C (staining time of 1 minute, staining solution to buffer ratio of 1:18, and staining solution to buffer temperature of 35 degrees Celsius). The slide pushing mechanism pushes out four sample smears, namely A, B, C and D; then the slide pushing and staining device selects sample smear A to be stained according to the first staining parameter; sample smear B to be stained according to staining parameter A; sample smear C to be stained according to staining parameter B; and sample smear D to be stained according to staining parameter C.

[0073] Please refer to details. Figure 8 As shown, one embodiment of the staining method for sample staining in this application includes:

[0074] 801, obtain sample information of the sample to be tested.

[0075] The staining device receives sample information of the sample to be tested sent by the sample analyzer or directly obtains sample information of the sample to be tested input by the user through the input device.

[0076] In this embodiment, the sample information includes at least one of the component information of the sample to be tested, the sample type of the sample to be tested, the source information of the sample to be tested, and the application scenario information of the sample to be tested. The component information (also referred to as cell detection parameters) of the sample to be tested includes hemoglobin information, white blood cell information, and platelet information. The cell detection parameter information can be used to indicate whether the corresponding cell detection parameter value is abnormal or normal. In this embodiment, abnormal cell detection parameter values ​​mainly indicate that the corresponding cell detection parameter value does not meet the corresponding threshold (e.g., greater than the threshold or outside the threshold range), the cell detection parameter is missing (e.g., the cell detection parameter is empty or the corresponding cell detection parameter value is the initial value), or the corresponding cell detection parameter value directly indicates whether there is an abnormality (e.g., it does not need to be determined by a threshold method), etc., are all considered abnormal cell detection parameter values. Conversely, it indicates that the corresponding cell detection parameter value is normal.

[0077] The sample type is used to indicate whether the sample is blood, body fluid, or bone marrow fluid.

[0078] The source information of the sample to be tested includes the information of the person to be tested corresponding to the sample or the department from which the sample originated. For example, the patient's medical history may show that the patient has leukemia; or the sample may originate from a specific department, such as the test results, which may originate from, but are not limited to, the reproductive medicine department. The specific department can be determined according to medical needs, and this embodiment does not impose any limitations.

[0079] The application scenario information of the sample includes the detection items corresponding to the sample. For example, the sample to be tested may only detect white blood cells or only detect platelets, etc. Specific situations are not limited here.

[0080] 802, control the staining mechanism to select staining parameters according to the sample information to stain the sample to be measured on the sample smear.

[0081] When the staining device determines that the sample to be tested is a special sample or an abnormal sample based on the sample information, the staining device controls the staining mechanism to determine the first staining parameter corresponding to the sample to be tested according to a preset mapping table, and uses the staining parameter to stain the sample to be tested on the sample smear; if the staining device determines that the sample to be tested is a normal sample based on the sample information, the staining device controls the staining mechanism to stain the sample to be tested on the sample smear according to the conventional staining parameters.

[0082] In this embodiment, the preset mapping table is used to indicate the correspondence between samples and staining parameters. In one example, the preset mapping table is shown in Table 2 below:

[0083] Table 2

[0084] Sample Information Staining time The ratio of staining solution to buffer solution body fluid 2 minutes 1：18 High white blood cell count 8 minutes 1：6 Low white blood cell count sample 1 minute 1：18

[0085] The staining time and the ratio of staining solution to buffer can be set in advance as needed. The staining device will automatically select the required staining parameters based on the sample information. The above staining scheme is just one example; in practice, more levels can be defined, such as setting multiple staining parameters based on different white blood cell count ranges.

[0086] In this embodiment of the application, the staining device specifically stains the sample to be tested on the sample smear according to the first staining parameters, including the following possible implementation methods:

[0087] In one possible implementation, the staining device needs to stain the multiple sample smears. In this case, the staining device controls the staining mechanism to stain at least one sample smear according to the first staining parameters, while the remaining sample smears are stained using conventional staining parameters. For example, if the slide pushing mechanism pushes out three sample smears, A, B, and C, the staining device selects sample smear A to be stained according to the first staining parameters, while the remaining sample smears B and C are stained using conventional staining parameters.

[0088] In another implementation, after determining the first staining parameters of the sample to be tested, the staining device adjusts the first staining parameters within a preset range to obtain a set of staining parameters; then, it controls the staining mechanism to stain any one sample smear according to the first staining parameters, and controls the staining mechanism to stain the remaining sample smears according to each set of staining parameters in the set of staining parameters. For example, the first staining parameters of the sample to be tested are: staining time of 2 minutes, staining solution to buffer ratio of 1:18, and staining solution to buffer temperature of 30 degrees Celsius. In an exemplary scheme, the set of staining parameters includes: staining parameter A (staining time of 2 minutes, staining solution to buffer ratio of 1:16, and staining solution to buffer temperature of 32 degrees Celsius), staining parameter B (staining time of 1 minute, staining solution to buffer ratio of 1:16, and staining solution to buffer temperature of 31 degrees Celsius), and staining parameter C (staining time of 1 minute, staining solution to buffer ratio of 1:18, and staining solution to buffer temperature of 35 degrees Celsius). The slide pushing mechanism pushes out four sample smears, namely A, B, C and D; then the staining device selects sample smear A to be stained according to the first staining parameter; sample smear B to be stained according to staining parameter A; sample smear C to be stained according to staining parameter B; and sample smear D to be stained according to staining parameter C.

[0089] The sample staining process in this application embodiment is illustrated below using a slide staining device as an example:

[0090] Please refer to details. Figure 9 As shown, one staining process of the slide staining device is as follows:

[0091] The sample analyzer performs sample analysis tests on the sample to be tested to obtain sample information; the sample analyzer sends the sample information to the slide staining device; the slide staining device determines whether the sample to be tested is a special sample based on the sample information; if so, the control mechanism controls the slide pushing mechanism to push two sample smears, and the control mechanism controls the staining mechanism to stain one sample smear according to the conventional staining parameters, and to stain the other smear according to the first staining parameters corresponding to the sample to be tested; if not, the control mechanism controls the slide pushing mechanism to push out one sample smear and to stain it according to the conventional staining parameters.

[0092] Please refer to details. Figure 10 As shown, another staining process of the slide staining device:

[0093] The sample analyzer performs sample analysis tests on the sample to be tested to obtain sample information; the sample analyzer sends the sample information to the slide staining device; the slide staining device determines whether the sample to be tested is a special sample based on the sample information; if so, the control mechanism controls the slide pushing mechanism to push a sample smear, and the control mechanism controls the staining mechanism to stain according to the first staining parameters corresponding to the sample to be tested; if not, the control mechanism controls the slide pushing mechanism to push out a sample smear and stain according to the conventional staining parameters.

[0094] It is understood that in this embodiment, the staining methods include drop staining, single-slide immersion staining, and multi-slide staining. In the drop staining method, a mixture of dye and buffer solution is dropped onto the sample smear. During continuous staining, the staining time and the ratio of dye to buffer solution in the mixture can be changed as needed. In the single-slide immersion staining method, one sample smear is placed in a staining container, and the corresponding dye or mixture is added for staining. Therefore, similar to drop staining, the staining time and the ratio of dye to buffer solution in the mixture can be changed as needed. In the multi-slide staining method, multiple sample smears are placed in one staining container for staining. Therefore, during continuous staining, the ratio of dye to buffer solution cannot be adjusted in real time. If the ratio of dye to buffer solution needs to be changed, the next smear can only be stained after the current staining process is complete, which is time-consuming. Therefore, multi-slide staining is not suitable for adjusting the ratio of dye to buffer solution in the mixture; only the staining time can be adjusted.

[0095] The above describes the sample staining method in the embodiments of this application. In the embodiments of this application, the slide staining device is as follows: Figure 3 As described above, it will not be repeated here. The staining apparatus possesses... Figure 3 The full functions of the dyeing mechanism will not be elaborated here.

[0096] This application also provides a sample analysis system, please refer to the following for details. Figure 11 As shown, the sample analysis system includes a sample analysis device 1101, which is used to detect the sample to be tested and obtain sample information;

[0097] The slide-staining apparatus 1102 includes a slide-pushing mechanism and a staining mechanism. The slide-pushing mechanism is configured to prepare a sample smear, and the staining mechanism is configured to stain the sample to be tested on the sample smear.

[0098] Cell image analysis device 1103 is configured to capture and analyze sample smears;

[0099] The transport device 1104 includes a first transport track, a second transport track, and a third transport track. The first transport track is used to transport samples from the sample analysis device to the slide pushing mechanism; the second transport track is used to transport sample smears from the slide pushing mechanism to the staining mechanism; and the third transport track is used to transport sample smears from the staining mechanism to the cell image analysis device.

[0100] The control mechanism 1105 is communicatively connected to the sample analysis device 1101, the slide pushing mechanism, the staining mechanism, the cell image analysis device 1103, and the transport device 1104, and is configured to:

[0101] The sample information is obtained from the sample analysis device 1101; the first transport track is controlled to transport the sample to be tested to the slide pushing mechanism; the slide pushing mechanism is controlled to prepare a sample smear, and the second transport track is controlled to send the sample smear to the staining mechanism; the staining mechanism is controlled to select staining parameters according to the sample information to stain the sample to be tested on the sample smear; the third transport track is controlled to transport the sample smear from the staining mechanism to the cell image analysis device 1103 for imaging and analysis.

[0102] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working processes of the systems, devices, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here.

[0103] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection between apparatuses or units through some interfaces, and may be electrical, mechanical, or other forms.

[0104] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0105] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0106] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of this application. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0107] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit it. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A staining method for sample staining, applied to a slide staining apparatus, the slide staining apparatus comprising: a slide pushing mechanism for applying a sample to be tested onto a glass slide to form a sample smear; a staining mechanism for staining the sample to be tested on the sample smear; and a transport mechanism for transporting the sample smear from the slide pushing mechanism to the staining mechanism, characterized in that... include: Obtain the sample information of the sample to be tested, the sample information including sample type or sample source information, the sample type including blood, body fluid or bone marrow fluid, the sample source information including the information of the person to be tested or the department from which the sample was sourced; The slide pushing mechanism is controlled to prepare sample smears according to the sample information; The transport mechanism is controlled to transport the sample smear from the slide pushing mechanism to the staining mechanism; The staining mechanism is controlled to select a first staining parameter or a conventional staining parameter to stain the sample to be tested on the sample smear according to the sample information. The first staining parameter is determined according to a preset mapping table, which is used to indicate the correspondence between the sample and the staining parameter. The conventional staining parameter includes the staining parameters pre-configured by the slide-pushing staining device.

2. The method according to claim 1, characterized in that, The process of controlling the staining mechanism to select staining parameters based on sample information to stain the sample smear for testing includes: If the sample information indicates that the sample to be tested is a special sample, the staining mechanism is controlled to stain the sample to be tested on the sample smear according to the first staining parameters. The special sample includes the sample to be tested originating from the person to be tested corresponding to a case of abnormal white blood cells, or the sample to be tested originating from the reproductive medicine department, or the sample to be tested being a bodily fluid. If the sample information indicates that the sample to be tested is a routine sample, the staining mechanism is controlled to stain the sample to be tested on the sample smear according to the routine staining parameters.

3. The method according to any one of claims 1-2, characterized in that, The step of controlling the slide pushing mechanism to prepare a sample smear based on the sample information includes: If the sample information indicates that the sample to be tested is a special sample, the slide pushing mechanism is controlled to prepare at least one sample smear. The special sample includes the sample to be tested originating from the person to be tested corresponding to a case of abnormal white blood cells, or the sample to be tested originating from the reproductive medicine department, or the sample to be tested being a bodily fluid. If the sample information indicates that the sample to be tested is a regular sample, control the slide pushing mechanism to prepare a sample smear.

4. The method according to claim 3, characterized in that, The number of sample smears is at least two, and controlling the staining mechanism to select staining parameters on the sample smears according to the sample information includes: If the sample information indicates that the sample to be tested is a special sample, the staining mechanism is controlled to stain the sample on one sample smear randomly selected from the at least two sample smears according to the first staining parameters; and to stain the samples on other sample smears in the sample smears according to the conventional staining parameters.

5. The method according to claim 3, characterized in that, The number of sample smears is at least two, and controlling the staining mechanism to select staining parameters on the sample smears according to the sample information includes: A set of staining parameters is obtained by adjusting the first staining parameter within a preset range. The set of staining parameters includes multiple sets of staining parameters. The first staining parameter is determined according to a preset mapping table, which is used to indicate the correspondence between samples and staining parameters. The staining mechanism is controlled to stain the sample on any one of the at least two sample smears according to the first staining parameter, and to stain the samples on the other sample smears according to each set of staining parameters in the set of staining parameters.

6. The method according to any one of claims 1 to 2, or any one of claims 4 to 5, characterized in that, The process of obtaining the sample information of the sample to be tested includes: The sample information sent by the sample analyzer is obtained, and the sample information is obtained by the sample analyzer. or, Receive the sample information input via an input device.

7. The method according to any one of claims 1 to 2, or any one of claims 4 to 5, characterized in that, The sample information also includes at least one of the component information of the sample to be tested, as well as the application scenario information of the sample to be tested.

8. The method according to any one of claims 1 to 2, or any one of claims 4 to 5, characterized in that, The staining parameters include staining time, the ratio of staining solution to buffer solution, and temperature.

9. A staining method for staining samples, applied to a staining apparatus including a staining mechanism, said staining mechanism being used to stain a sample to be tested on a sample smear, characterized in that, include: Obtain sample information of the sample to be tested, including sample type or sample source information. The sample type includes blood, body fluid or bone marrow fluid, and the sample source information includes the information of the person to be tested or the department from which the sample was sourced. The staining mechanism is controlled to select a first staining parameter or a conventional staining parameter to stain the sample to be tested on the sample smear according to the sample information. The first staining parameter is determined according to a preset mapping table, which is used to indicate the correspondence between the sample and the staining parameter. The conventional staining parameter includes the staining parameters pre-configured by the slide-pushing staining device.

10. The method according to claim 9, characterized in that, The process of controlling the staining mechanism to select staining parameters based on sample information to stain the sample smear for testing includes: If the sample information indicates that the sample to be tested is a special sample, the staining mechanism is controlled to stain the sample to be tested on the sample smear according to the first staining parameters. The special sample includes the sample to be tested originating from the person to be tested corresponding to a case of abnormal white blood cells, or the sample to be tested originating from the reproductive medicine department, or the sample to be tested being a bodily fluid. If the sample information indicates that the sample to be tested is a routine sample, the staining mechanism is controlled to stain the sample to be tested on the sample smear according to the routine staining parameters.

11. The method according to any one of claims 9 to 10, characterized in that, The process of controlling the staining mechanism to select staining parameters based on sample information to stain the sample smear for testing includes: If the sample information indicates that the sample to be tested is a special sample, the number of sample smears is at least two, and the staining mechanism is controlled to stain the sample on one of the sample smears randomly selected from the at least two sample smears according to the first staining parameter; and to stain the samples on other sample smears in the sample smear according to the conventional staining parameter. The special sample includes the sample to be tested originating from the person to be tested corresponding to a case of abnormal white blood cells, or the sample to be tested originating from the reproductive medicine department, or the sample to be tested being a bodily fluid.

12. The method according to any one of claims 9 to 10, characterized in that, The process of controlling the staining mechanism to select staining parameters based on sample information to stain the sample smear for testing includes: If the sample information indicates that the sample to be tested is a special sample and the number of sample smears is at least two, the staining mechanism is controlled to stain the sample on any one of the at least two sample smears according to the first staining parameter. The special sample includes the sample to be tested originating from the person to be tested corresponding to a case of abnormal white blood cells, or the sample to be tested originating from the reproductive medicine department, or the sample to be tested being a bodily fluid. The first staining parameter is adjusted within a preset range to obtain a staining parameter set, which includes multiple sets of staining parameters. The staining mechanism is controlled to stain the samples on other sample smears in the sample smear according to each set of staining parameters in the staining parameter set.

13. The method according to any one of claims 9 to 10, characterized in that, The process of obtaining the sample information of the sample to be tested includes: The sample information sent by the sample analyzer is obtained, and the sample information is obtained by the sample analyzer. or, Receive the sample information input via an input device.

14. The method according to any one of claims 9 to 10, characterized in that, The sample information includes at least one of the component information of the sample, as well as the application scenario information of the sample.

15. The method according to any one of claims 9 to 10, characterized in that, The staining parameters include staining time, the ratio of staining solution to buffer solution, and temperature.

16. A slide-staining apparatus, characterized in that, include: A slide-pushing mechanism is used to apply the sample to a glass slide to prepare a sample smear. A staining facility is used to stain samples on the sample smear according to sample information. The staining facility has special staining modes and ordinary staining modes. The sample information includes sample type or sample source information. The sample type includes blood, body fluid or bone marrow fluid. The sample source information includes the information of the person to be tested or the department from which the sample was sourced. A transport mechanism for transporting the sample smear to the staining facility; A control mechanism, communicatively connected to the slide pushing mechanism, staining mechanism, and transport mechanism, is configured to: determine the operating mode of the staining mechanism from a special staining mode and a normal staining mode based on the sample information; when the operating mode is determined to be a special staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to a first staining parameter, the first staining parameter being determined according to a preset mapping table, the preset mapping table indicating the correspondence between the sample and the staining parameter; the special staining mode indicating that the source of the sample to be tested is the person to be tested corresponding to a case of abnormal white blood cells or that the source department of the sample to be tested is a reproductive medicine department, or indicating that the sample to be tested is a body fluid; when the operating mode is determined to be a normal staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to conventional staining parameters, the normal staining mode indicating that the sample to be tested is a normal blood sample, the conventional staining parameters being the staining parameters pre-configured by the slide pushing and staining device.

17. The apparatus according to claim 16, characterized in that, The slide pushing mechanism also includes a special slide preparation mode and a regular slide preparation mode. The control mechanism is further configured to: determine the operating mode of the slide pushing mechanism from the special slide preparation mode and the regular slide preparation mode; when the operating mode is determined to be the special slide preparation mode, control the slide pushing mechanism to prepare at least two sample smears; when the operating mode is determined to be the regular slide preparation mode, control the slide pushing mechanism to prepare one sample smear.

18. A sample staining apparatus, characterized in that, include: A staining facility is used to stain samples on sample smears according to sample information. The staining facility has special staining modes and ordinary staining modes. The sample information includes sample type or sample source information. The sample type includes blood, body fluid or bone marrow fluid. The sample source information includes the information of the person to be tested or the department from which the sample was sourced. A control mechanism, communicatively connected to the staining mechanism, is configured to: determine the operating mode of the staining mechanism from a special staining mode and a normal staining mode based on the sample information; when the operating mode is determined to be a special staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to a first staining parameter, the first staining parameter being determined according to a preset mapping table, the preset mapping table indicating the correspondence between the sample and the staining parameter; the special staining mode indicating that the source of the sample to be tested is the person to be tested corresponding to a case of abnormal white blood cells or that the source department of the sample to be tested is the reproductive medicine department, or indicating that the sample to be tested is body fluid; when the operating mode is determined to be a normal staining mode, control the staining mechanism to stain the sample to be tested on the sample smear according to conventional staining parameters, the normal staining mode indicating that the sample to be tested is a normal blood sample, the conventional staining parameters being the staining parameters pre-configured by the staining device.

19. A sample analysis system, characterized in that, The sample analysis system includes: A sample analysis device is used to test samples and obtain sample information; A slide-staining apparatus includes a slide-pushing mechanism and a staining mechanism, wherein the slide-pushing mechanism is configured to prepare a sample smear, and the staining mechanism is configured to stain the sample to be tested on the sample smear; A cell image analysis device is designed for capturing and analyzing sample smears; The transport device includes a first transport track, a second transport track, and a third transport track. The first transport track is used to transport samples from the sample analysis device to the slide pushing mechanism; the second transport track is used to transport sample smears from the slide pushing mechanism to the staining mechanism; and the third transport track is used to transport sample smears from the staining mechanism to the cell image analysis device. A control mechanism, communicatively connected to the sample analysis device, the slide pushing mechanism, the staining mechanism, the cell image analysis device, and the transport device, and configured to: The sample information is obtained from the sample analysis device. The sample information includes sample type or sample source information. The sample type includes blood, body fluid or bone marrow fluid. The sample source information includes the information of the person to be tested or the department from which the sample was sourced. The system controls the first transport track to transport the sample to be tested to the slide pushing mechanism; controls the slide pushing mechanism to prepare a sample smear; and controls the second transport track to deliver the sample smear to the staining mechanism; controls the staining mechanism to select a first staining parameter or a conventional staining parameter to stain the sample to be tested on the sample smear according to the sample information; and controls the third transport track to transport the sample smear from the staining mechanism to the cell image analysis device for imaging and analysis. The first staining parameter is determined according to a preset mapping table, which indicates the correspondence between the sample and the staining parameter. The conventional staining parameter includes the staining parameters pre-configured by the slide pushing and staining device.

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