Sample analyzer and its detection methods

Abstract

This invention provides a sample analyzer and its detection method. The sample analyzer includes a sheath flow impedance detection component, an RBC reaction component, a tubing component, an optical reaction component, an optical detection component, a sampling component, and a syringe. The RBC reaction component processes the sample to be tested to form a first sample solution, and the sheath flow impedance detection component detects the first sample solution. The optical reaction component processes the sample to be tested to form a second sample solution, and the optical detection component detects the second sample solution. The syringe is connected to the sheath flow impedance detection component, the RBC reaction component, the optical reaction component, the optical detection component, and the sampling component through the tubing component, and continuously works in conjunction with these components. This reduces the cost and size of the instrument.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a sample analyzer and its testing method. Background Technology

[0002] In a five-part differential hematology analyzer, the liquid circuit typically includes a sampling power source, an optical channel sample pushing power source, and a sheath flow impedance channel sample pushing power source. These power sources are usually implemented using syringes.

[0003] There are generally two solutions for the power source of the sampling syringe: one is a model that includes a blood dispensing valve, which has a large blood collection volume, but the quantification and blood dispensing are achieved by the blood dispensing valve. Therefore, the quantitative accuracy requirement of the syringe is not high. In this case, a syringe with a larger volume range, such as a milliliter syringe, is generally used. The other is a model that uses a syringe to dispense blood, which has a smaller blood collection volume. The quantification and blood dispensing are achieved by the syringe. The quantitative accuracy requirement of the syringe is high. In this case, a syringe with a smaller volume range, such as a microliter syringe, is generally used.

[0004] For optical channel measurements, the sample flow rate is very low and requires stable flow, so a micro-lift syringe is generally used. However, optical channels measure different types of cells, such as white blood cells, red blood cells, and PLT, which have huge differences in cell volume and sample concentration. This results in a large difference between the flow rate and the volume of the sample (for example, a large sample volume is required when measuring white blood cells, and a large sample volume is required when measuring low-value or pre-diluted samples). Therefore, in order to balance quantitative accuracy and sample volume, a micro-lift syringe with a medium range is generally used.

[0005] For sheath flow impedance measurement, the sample flow rate is very low and requires stable flow, so a micro-lift syringe is generally used; the channel measures a single concentration and the measurement volume is small, so a small-range micro-lift syringe is generally used.

[0006] Currently, in five-part differential hematology analyzers, due to limitations in measurement speed or incompatibility in syringe performance, separate syringe assemblies are typically provided for each channel to meet the sample delivery requirements of the corresponding module. However, the large number of syringes used in these five-part differential hematology analyzers leads to high instrument costs and large size, hindering miniaturization and / or integrated design. Summary of the Invention

[0007] Therefore, it is necessary to provide a sample analyzer and its detection method that reduces cost and size, addressing the current problem of high instrument cost and large size caused by the large number of syringes.

[0008] The above objectives are achieved through the following technical solutions:

[0009] A sample analyzer includes a sheath flow impedance detection assembly, an RBC reaction assembly, a tubing assembly, an optical reaction assembly, an optical detection assembly, a sampling assembly, and a syringe;

[0010] The sampling component is used to collect the sample to be tested; the RBC reaction component is used to process the sample to be tested to form a first sample solution; the sheath current impedance detection component is used to detect the first sample solution; the optical reaction component is used to process the sample to be tested to form a second sample solution; and the optical detection component is used to detect the second sample solution.

[0011] The syringe is connected to the sheath flow impedance detection component, the RBC reaction component, the optical reaction component, the optical detection component, and the sampling component through the tubing assembly, and works continuously in conjunction with the sampling component, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component, and the optical detection component.

[0012] In one embodiment, during continuous operation of the syringe, the syringe can sample and separate the sample to be tested, and the syringe can also push the first sample liquid into the sheath flow impedance detection component and push the second sample liquid into the optical detection component.

[0013] In one embodiment, the tubing assembly includes a first connecting tubing, a second connecting tubing, and a third connecting tubing. The first connecting tubing connects the sampling component to the syringe. One end of the second connecting tubing is connected to the first connecting tubing, and the other end of the second connecting tubing is connected to the sheath current impedance detection component. One end of the third connecting tubing is connected to the first connecting tubing, and the other end of the third connecting tubing is connected to the optical detection component.

[0014] In one embodiment, the piping assembly further includes a first switching element, which enables the connection and disconnection of the first connecting pipe with the second connecting pipe and the third connecting pipe.

[0015] In one embodiment, the first switching element is disposed at the connection between the first connecting pipe and the second connecting pipe and the third connecting pipe. The first switching element has three leads, one of which is connected to the first connecting pipe, and the other two leads are respectively connected to the first connecting pipe and the second connecting pipe.

[0016] In one embodiment, the first connecting pipe has a first access point, which is connected to the second connecting pipe and the third connecting pipe respectively; the first switching component includes a first sub-switching component and a second sub-switching component, the first sub-switching component is disposed in the second connecting pipe, and the second sub-switching component is disposed in the third connecting pipe.

[0017] In one embodiment, the sampling assembly includes a sampling needle, a sampling tubing, and a second switching element. The sampling tubing connects the sampling needle and the second switching element, and the first connecting tubing connects the second switching element and the syringe. The syringe and the second switching element cooperate to sample and separate the sample to be tested.

[0018] In one embodiment, the first connecting pipe further has a second access point connected to the sampling pipe, and the second switching element is disposed on the sampling pipe.

[0019] In one embodiment, the second switching element is disposed at the connection between the sampling pipeline and the first connecting pipeline.

[0020] In one embodiment, the second connecting conduit has a third access point, through which the second connecting conduit is connected to the sheath current impedance detection component and the RBC reaction component respectively.

[0021] In one embodiment, the RBC reaction assembly includes an RBC reaction cell, and the pipeline assembly further includes an RBC sample preparation pipeline and a third switching element. The RBC reaction cell is used to process the sample to be tested to form a first sample solution. The RBC sample preparation pipeline connects the third access point to the RBC reaction cell, and the third switching element is disposed on the RBC sample preparation pipeline.

[0022] In one embodiment, the third connection conduit has a fifth access point, through which the third connection conduit connects the optical detection component and the optical reaction component.

[0023] In one embodiment, the optical reaction assembly includes an optical reaction cell; the pipeline assembly further includes an optical sample preparation pipeline and a fifth switching element, the optical reaction cell is used to process the sample to be tested to form a second sample liquid, the optical sample preparation pipeline connects the fifth access point to the optical reaction cell, and the fifth switching element is disposed on the optical sample preparation pipeline.

[0024] In one embodiment, the syringe has a volume range of 100uL to 300uL.

[0025] A detection method for a sample analyzer includes the following steps:

[0026] The sampling component is moved above the sample to be tested, and the syringe is controlled to drive the sampling component to collect the sample.

[0027] The sampling component is controlled to move to the RBC reaction component, and the syringe is controlled to drive the sampling component to deliver the sample to be tested into the RBC reaction component, where the RBC reaction component processes the sample to be tested to form a first sample solution.

[0028] The sampling component is controlled to move to the optical reaction component, and the syringe is controlled to drive the sampling component to deliver the sample to be tested into the optical reaction component, whereby the optical reaction component processes the sample to be tested to form a second sample solution.

[0029] The first sample solution in the RBC reaction assembly is allowed to flow, and the syringe pushes the first sample solution into the sheath flow impedance detection assembly;

[0030] The second sample liquid in the optical reaction assembly is allowed to flow, and the syringe pushes the second sample liquid into the optical detection assembly;

[0031] Within one measurement cycle, the syringe continuously works in conjunction with the sampling component, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component, and the optical detection component.

[0032] By adopting the above technical solution, the present invention has at least the following technical effects:

[0033] The sample analyzer and its detection method of the present invention use a syringe connected to a sampling component, an RBC reaction component and a sheath current impedance detection component via a tubing assembly. During the measurement process, the syringe continuously works with the sampling component, the RBC reaction component and the sheath current impedance detection component, thereby realizing the reuse of the syringe. This effectively solves the problem of high instrument cost and large size caused by a large number of syringes, significantly reducing the cost and size of the instrument and facilitating instrument miniaturization and / or integrated design. Attached Figure Description

[0034] Figure 1 This is a liquid circuit connection diagram of the sample analyzer according to the first embodiment of the present invention;

[0035] Figure 2 This is a liquid circuit connection diagram of the sample analyzer according to the second embodiment of the present invention;

[0036] Figure 3 This is a liquid circuit connection diagram of the sample analyzer according to the third embodiment of the present invention;

[0037] Figure 4This is a liquid circuit connection diagram of the sample analyzer according to the fourth embodiment of the present invention;

[0038] Figure 5 This is a liquid circuit connection diagram of the sample analyzer according to the fifth embodiment of the present invention;

[0039] Figure 6 This is a schematic diagram of the measurement cycle of the sample analyzer of the present invention.

[0040] Wherein: 100, Sample analyzer; 110, Syringe; 120, Sampling assembly; 121, Sampling needle; 122, Sampling tubing; 123, Second switching element; 130, Tubing assembly; 131, First connecting tubing; S1, First access point; 132, Second connecting tubing; S3, Third access point; 133, Third connecting tubing; S5, Fifth access point; 134, First switching element; 1341, First sub-switching element; 1342, Second sub-switching element; 135, RBC sample preparation tubing; 136, Third switching element; 137, Optical sample preparation tubing; 138, Fifth switching element; 140, RBC reaction cell; 150, Sheath current impedance detection assembly; 151, Sheath current impedance detection unit; 152, Sheath current impedance 153. Sample preparation power source for sheath flow impedance detection sample; 154. Sample preparation pipeline for sheath flow impedance detection sample; 1541. First preparation liquid section; 1542. First negative pressure power source connection pipeline; 155. Sheath liquid chamber; 156. Fourth switching element; S4. Fourth access point; 160. Optical reaction cell; 170. Optical detection assembly; 171. Optical detection unit; 172. Optical detection sample needle; 173. Optical detection sample preparation power source; 174. Optical detection pipeline; 1741. Second preparation liquid section; 1742. Second negative pressure power source connection pipeline; 175. Sixth switching element; S6. Sixth access point; 180. Diluent assembly; 181. Diluent chamber; 182. Diluent pipeline; 183. Seventh switching element. Detailed Implementation

[0041] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0042] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0043] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0044] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0045] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0046] It should be noted that when an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0047] See Figures 1 to 6 This invention provides a sample analyzer 100. The sample analyzer 100 is used to analyze and detect samples to obtain corresponding test results to meet usage requirements. It should be noted that the specific type of sample to be tested is not limited; in some embodiments, the sample to be tested includes solid samples or liquid samples. It is understood that when testing liquid samples, the liquid sample needs to be placed on a sample holder. Further liquid samples include, but are not limited to, blood samples.

[0048] This invention uses a blood sample as an example for illustration. Specifically, when the sample analyzer 100 tests a blood sample, the sample is stored in a test tube and placed sequentially on a test tube rack. The sample analyzer 100 of this invention is an in vitro diagnostic device suitable for performing measurements such as blood cell counting, white blood cell differential, hemoglobin concentration measurement, reticulocyte measurement, and nucleated red blood cell measurement under laboratory conditions.

[0049] The sample analyzer 100 of the present invention can realize continuous detection of multiple parameters of the sample to be tested. While ensuring that the detection efficiency meets the requirements of use, it can reduce the size of the instrument and reduce the cost of the instrument, which is conducive to the miniaturization / integration design of the sample analyzer 100.

[0050] See Figures 1 to 5 In one embodiment, the sample analyzer 100 includes a sheath flow impedance detection component 150, an RBC (Red Blood Cell) reaction component, a tubing component 130, an optical reaction component, an optical detection component 170, a sampling component 120, and a syringe 110.

[0051] The sampling component 120 is used to collect the sample to be tested, the RBC reaction component is used to process the sample to form a first sample solution, and the sheath flow impedance detection component 150 is used to detect the first sample solution; the optical reaction component is used to process the sample to form a second sample solution, and the optical detection component 170 is used to detect the second sample solution. The syringe 110 is connected to the sheath flow impedance detection component 150, the RBC reaction component, the optical reaction component, the optical detection component 170, and the sampling component 120 through the tubing assembly 130, and works continuously in conjunction with the sampling component 120, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component 150, and the optical detection component 170.

[0052] The sample analyzer 100 has a syringe 110, which is connected to the sheath current impedance detection component 150, the RBC reaction component, the tubing component 130, the optical reaction component, the optical detection component 170, and the sampling component 120, respectively. The syringe 110 can cooperate with the sheath current impedance detection component 150, the RBC reaction component, the tubing component 130, the optical reaction component, the optical detection component 170, and the sampling component 120 to realize the analysis and detection operation of the sample analyzer 100 on the sample to be tested.

[0053] After the syringe 110 is connected to the sampling component 120, the withdrawal operation of the syringe 110 can control the sampling component 120 to aspirate the sample to be tested, so that the sample to be tested is stored in the sampling component 120, thus realizing the sampling function of the sampling component 120. Specifically, the sample analyzer 100 has a sampling position. After the sample to be tested is delivered to the sampling position of the sample analyzer 100, the sampling component 120 can aspirate the sample to be tested at the sampling position, completing the sampling operation of the sampling component 120. It can be understood that the sample to be tested can be delivered to the sampling position by medical personnel directly moving the test tube of the sample to be tested to the sampling position, or the sample transfer component of the sample analyzer 100 can deliver the test tube containing the sample to be tested. This sample transfer component can adopt the structure of current blood analyzers.

[0054] The sampling component 120 can also be moved to the RBC reaction component to deliver the sample to be tested, and to the optical reaction component to deliver the sample to be tested to both the RBC reaction component and the optical reaction component, meaning the sampling component 120 can perform sample separation. The separation function of the sampling component 120 is controlled by the syringe 110. The pushing operation of the syringe 110 controls the output of the sample to be tested from the sampling component 120, achieving sample separation. The syringe 110 ensures quantitative accuracy in sample separation, ensuring that the sample separated into the RBC reaction component and the optical reaction component meets the actual testing requirements while avoiding waste.

[0055] The sample analyzer 100 also includes a main controller and a sampling drive unit. The sampling drive unit is connected to the sampling assembly 120 and can drive the sampling assembly 120 to move between the sampling position, the RBC reaction assembly, and the optical reaction assembly. Specifically, the main controller can control the sampling drive unit to move the sampling assembly 120 to the sampling position and control the sampling assembly 120 to insert into the test tube; the main controller can also control the sampling drive unit to move the sampling assembly 120 to the RBC reaction assembly; and the main controller can also control the sampling drive unit to move the sampling assembly 120 to the optical reaction assembly.

[0056] In principle, the sampling component 120 has no restriction on the sampling order between the RBC reaction component and the optical reaction component; it can sample first at the RBC reaction component or first at the optical reaction component. For example, the sampling component 120 can move to the RBC reaction component for sampling first, and then move to the optical reaction component for sampling. Since the sample needs to be incubated in the RBC reaction component after sampling by the sampling component 120, which requires a certain amount of time, controlling the sampling component 120 to sample to the RBC reaction component first allows for sampling to the optical reaction component during the sample incubation process. This shortens the waiting time of the syringe 110 and improves the processing efficiency of the sample analyzer 100.

[0057] The RBC reaction assembly and the sheath impedance detection assembly 150 work together to perform sheath impedance detection on the test sample. Specifically, the RBC reaction assembly receives the test sample dispensed by the sampling assembly 120 and can incubate the test sample to obtain a first sample solution for red blood cell detection. The sheath impedance detection assembly 150 uses negative pressure to extract the first sample solution from the RBC reaction assembly, and then uses a syringe 110 to push the first sample solution into the sheath impedance detection assembly 150. The sheath impedance detection assembly 150 then performs sheath impedance detection on the test sample to obtain red blood cell and platelet parameters.

[0058] The optical reaction assembly and the optical detection assembly 170 work together to perform optical detection on the sample to be tested. Specifically, the optical reaction assembly receives the sample to be tested dispensed by the sampling assembly 120 and can incubate the sample to obtain a second sample solution for detecting white blood cell parameters. The optical detection assembly 170 uses negative pressure to extract the second sample solution from the optical reaction assembly, and the syringe 110 pushes the second sample solution into the optical detection assembly 170. The optical detection assembly 170 then performs optical detection on the sample to obtain white blood cell parameters, such as detecting white blood cell count or white blood cell differential.

[0059] The sample analyzer 100 also includes an injection drive unit electrically connected to the main controller. This injection drive unit can drive the syringe 110 to perform pushing or aspiration operations. When the main controller controls the injection drive unit to drive the syringe 110 to perform an aspiration operation, the syringe 110 can cause the sampling component 120 to aspirate samples. When the main controller controls the injection drive unit to drive the syringe 110 to perform a pushing operation, the syringe 110 can cause the sampling component 120 to dispense samples, or push the first sample liquid into the sheath flow impedance detection component 150 and the second sample liquid into the optical detection component 170. Optionally, the injection drive unit is a stepper motor or other power source that can ensure quantitative accuracy.

[0060] The sample analyzer 100 of the present invention operates continuously in conjunction with a sampling component 120, an RBC reaction component, an optical reaction component, a sheath current impedance detection component 150, and an optical detection component 170 via a syringe 110. One measurement cycle of the sample analyzer 100 is as follows: the sampling component 120 moves to the sampling position, and the syringe 110 controls the sampling component 120 to aspirate the sample; subsequently, the sampling component 120 moves to the RBC reaction component, and the syringe 110 controls the sampling component 120 to dispense the sample into the RBC reaction component, which processes the sample to form a first sample solution; the sampling component 120 then moves to the optical reaction component, and the syringe 110 controls the sampling component 120 to dispense the sample into the optical reaction component, which processes the sample to form a second sample solution. Then, the first sample solution flows out of the RBC reaction component, and the syringe 110 pushes the first sample solution into the sheath current impedance detection component 150; the second sample solution flows out of the optical reaction component, and the syringe 110 pushes the second sample solution into the optical detection component 170, completing the detection of one sample.

[0061] Continuous coordination here refers to the continuous coordination between the syringe 110 and its components within one measurement cycle, such as... Figure 6 As shown, the actions of syringe 110 in aspirating, dispensing, and dispensing samples during the measurement cycle are continuous and sequential. After completing the aspiration operation, syringe 110 performs the dispensing operation, and then the dispensing operation. Understandably, because the movement of sampling component 120 and the transfer of sample liquid require a certain amount of time, syringe 110 remains stationary during this process. Syringe 110 operates only when sampling component 120, RBC reaction component, optical reaction component, sheath flow impedance detection component 150, and optical detection component 170 require operation.

[0062] It is worth noting that when there is only one sample to be tested, the sample analyzer 100 stops after detecting one sample. When there are multiple samples to be tested, the sample analyzer 100 performs multiple measurement cycles continuously.

[0063] The sample analyzer 100 of the above embodiment uses a syringe 110 connected to the sampling component 120, the RBC reaction component, and the sheath current impedance detection component 150 through the tubing assembly 130. During the measurement process, the syringe 110 continuously cooperates with the sampling component 120, the RBC reaction component, and the sheath current impedance detection component 150, realizing the reuse of the syringe 110. This effectively solves the problem of high instrument cost and large size caused by a large number of syringes 110, significantly reducing the cost and size of the instrument, and facilitating the miniaturization and / or integrated design of the instrument.

[0064] It is worth noting that the sample analyzer 100 of the present invention is not limited to sheath flow impedance detection and optical detection of the sample to be tested, but can also perform other measurements on the sample to be tested. In this case, a corresponding detection component and a reaction component that cooperates with the detection component are added to the sample analyzer 100, and the sampling component 120 can also dispense the sample into the reaction component, and the syringe 110 cooperates with the detection component to perform detection.

[0065] In one embodiment, the sample analyzer 100 further includes a reagent supply component. The reagent supply component provides processing reagents to the RBC reaction component and the optical reaction component, thereby mixing the blood sample to be tested with the processing reagents provided by the reagent supply component to prepare a sample solution. Specifically, the reagent supply component provides processing reagents to both the RBC reaction component and the optical reaction component. The sample to be tested in the RBC reaction component reacts with the processing reagents to form a first sample solution, and the sample to be tested in the optical reaction component reacts with the processing reagents to form a second sample solution.

[0066] In some embodiments, the reagent supply assembly includes a first reagent supply unit for supplying red blood cell reagent, which may be, for example, a diluent. In some embodiments, the reagent supply assembly includes a second reagent supply unit for supplying white blood cell reagent, which may include, for example, a hemolytic agent capable of dissolving red blood cells in a blood sample, and optionally, a fluorescent reagent capable of staining white blood cells. In other embodiments, the reagent supply assembly includes a third reagent supply unit for supplying hemoglobin reagent, which may be, for example, a hemolytic agent capable of dissolving red blood cells in a blood sample, releasing hemoglobin from the red blood cells, and converting hemoglobin into methemoglobin. In some embodiments, the white blood cell reagent and the hemoglobin reagent are the same hemolytic agent, i.e., the second and third reagent supply units are the same reagent supply unit.

[0067] See Figures 1 to 5In one embodiment, during continuous operation, the syringe 110 can sample and dispense the sample to be tested. The syringe 110 can also push the first sample liquid into the sheath flow impedance detection component 150 and the second sample liquid into the optical detection component 170. That is, within one cycle, the syringe 110 continuously works in conjunction with the sampling component 120, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component 150, and the optical detection component 170, achieving reuse of the syringe 110 and enabling it to perform multiple functions. This reduces the number of syringes 110, thereby reducing the size and cost of the sample analyzer 100.

[0068] Specifically, syringe 110 controls sampling component 120 to aspirate the sample to be tested, thus achieving sample sampling. Syringe 110 also controls sampling component 120 to dispense the sample, thus achieving sample separation. When sheath current impedance detection component 150 aspirates the first sample solution from the RBC reaction component, syringe 110 pushes the first sample solution into sheath current impedance detection component 150. When optical detection component 170 aspirates the second sample solution from the optical reaction component, syringe 110 pushes the second sample solution into the optical detection component 170, thus achieving sample dispensing of the first and second sample solutions. In other words, a single syringe 110 achieves aspiration, separation, and dispensing functions, significantly reducing instrument cost and size.

[0069] See Figures 1 to 5 In one embodiment, the tubing assembly 130 includes a first connecting tubing 131, a second connecting tubing 132, and a third connecting tubing 133. The first connecting tubing 131 connects the sampling assembly 120 and the syringe 110. One end of the second connecting tubing 132 is connected to the first connecting tubing 131, and the other end of the second connecting tubing 132 is connected to the sheath current impedance detection assembly 150. One end of the third connecting tubing 133 is connected to the first connecting tubing 131, and the other end of the third connecting tubing 133 is connected to the optical detection assembly 170.

[0070] The first connecting tube 131, the second connecting tube 132, and the third connecting tube 133 form a Y-shaped pathway. One end of the first connecting tube 131 is connected to the syringe 110, and the other end of the first connecting tube 131 is connected to the second connecting tube 132 and the third connecting tube 133, respectively. The sampling component 120 is connected to the first connecting tube 131. The end of the second connecting tube 132 away from the first connecting tube 131 is connected to the RBC reaction component and the sheath current impedance detection component 150, and the end of the third connecting tube 133 away from the first connecting tube 131 is connected to the optical reaction component and the optical detection component 170.

[0071] The syringe 110 is connected to the sampling component 120, the RBC reaction component, the optical reaction component, the sheath current impedance detection component 150, and the optical detection component 170 through the first connecting pipe 131, the second connecting pipe 132, and the third connecting pipe 133. In this way, the syringe 110 cooperates with each module through the above-mentioned pipes to realize the functions of sample aspiration, sample dispensing, and sample dispensing.

[0072] See Figures 1 to 5 In one embodiment, the piping assembly 130 further includes a first switching element 134, which enables the connection and disconnection of the first connecting pipe 131 with the second connecting pipe 132 and the third connecting pipe 133. The first switching element 134 controls the on / off relationship between the first connecting pipe 131 and the second connecting pipe 132 and the third connecting pipe 133. Optionally, the first switching element 134 is a valve or other component capable of on / off control. The first switching element 134 can switch the connection relationship of the first connecting pipe 131, select the on / off state of the first connecting pipe 131 and the second connecting pipe 132, and select the on / off state of the first connecting pipe 131 and the third connecting pipe 133.

[0073] Understandably, to ensure the accuracy of the syringe 110's operation, only one pathway can be kept open during operation, while the others are closed. For example, when the syringe 110 performs aspiration and dispensing operations, the first switching element 134 controls both the first connecting tube 131 and the second connecting tube 132 to be closed. At this time, the syringe 110 is connected to the sampling component 120, enabling sampling of the sample to be tested. When the syringe 110 performs a dispensing operation and pushes the first sample liquid, the first switching element 134 controls the first connecting tube 131 to be connected to the second connecting tube 132, and controls the first connecting tube 131 to be closed with the third connecting tube 133. When the syringe 110 performs a dispensing operation and pushes the second sample liquid, the second switching element 123 controls the first connecting tube 131 to be connected to the third connecting tube 133, and controls the first connecting tube 131 to be closed with the second connecting tube 132.

[0074] See Figure 1 and Figure 5In one embodiment, the first switching element 134 is disposed at the connection point of the first connecting pipe 131 with the second connecting pipe 132 and the third connecting pipe 133. The first switching element 134 has three leads, one of which is connected to the first connecting pipe 131, and the other two leads are respectively connected to the first connecting pipe 131 and the second connecting pipe 132. That is, the first switching element 134 is disposed at the connection point of the first connecting pipe 131 with the second connecting pipe 132 and the third connecting pipe 133, i.e., the first switching element 134 is disposed at the fork of the Y-shape. In this way, one end of the first switching element 134 is connected to the end of the first pipe away from the syringe 110, and the two leads of the first switching element 134 are respectively connected to the ends of the second connecting pipe 132 and the third connecting pipe 133. At this time, the first switching element 134 can realize the on / off control of the first connecting pipe 131 and the second connecting pipe 132, and can also realize the on / off control of the first connecting pipe 131 and the third connecting pipe 133.

[0075] For example, the first switching element 134 is a three-way valve. The three leads of the first switching element 134 are the three valve ports of the three-way valve, and the three leads are respectively connected to the first connecting pipe 131, the second connecting pipe 132, and the third connecting pipe 133, realizing the on / off control of the first connecting pipe 131, the second connecting pipe 132, and the third connecting pipe 133. The first switching element 134 is electrically connected to the main controller, and the main controller controls the on / off relationship of the first switching element 134 to the first connecting pipe 131, the second connecting pipe 132, and the third connecting pipe 133.

[0076] See Figures 2 to 3 In one embodiment, the first connecting pipe 131 has a first access point S1, which connects to the second connecting pipe 132 and the third connecting pipe 133 respectively. The first switching component 134 includes a first sub-switching component 1341 and a second sub-switching component 1342, with the first sub-switching component 1341 disposed on the second connecting pipe 132 and the second sub-switching component 1342 disposed on the third connecting pipe 133. Optionally, the first access point S1 is a tee or a component with three interfaces. The first access point S1 establishes the connection between the first connecting pipe 131, the second connecting pipe 132, and the third connecting pipe 133. After the first access point S1 is connected to the first connecting pipe 131, the second connecting pipe 132 and the third connecting pipe 133 can be connected to the first connecting pipe 131 through the first access point S1.

[0077] After connecting the first connecting pipe 131 to the second connecting pipe 132 and the third connecting pipe 133 through the first access point S1, the first connecting pipe 131 and the second connecting pipe 132 and the third connecting pipe 133 are always in a continuous state. To achieve on / off control of the first connecting pipe 131 and the second connecting pipe 132 and the third connecting pipe 133, the first switching component 134 includes a first sub-switching component 1341 and a second sub-switching component 1342. The first sub-switching component 1341 is disposed on the second connecting pipe 132, and the second sub-switching component 1342 is disposed on the third connecting pipe 133. The first sub-switching component 1341 controls the on / off state of the second connecting pipe 132, and the second sub-switching component 1342 controls the on / off state of the third connecting pipe 133.

[0078] For example, the first sub-switching element 1341 and the second sub-switching element 1342 are two-way valves. Further, the first sub-switching element 1341 is a normally open or normally closed two-way valve, and the second sub-switching element 1342 is a normally open or normally closed two-way valve. Of course, in other embodiments of the present invention, the first sub-switching element 1341 and the second sub-switching element 1342 may also be three-way valves or other valves capable of switching on and off.

[0079] See Figures 1 to 5 In one embodiment, the sampling assembly 120 includes a sampling needle 121, a sampling tube 122, and a second switching element 123. The sampling tube 122 connects the sampling needle 121 and the second switching element 123. A first connecting tube 131 connects the second switching element 123 and a syringe 110. The syringe 110 and the second switching element 123 cooperate to sample and dispense the test sample. The sampling needle 121 can be inserted into a test tube to draw the test sample. The sampling needle 121 is connected to the first connecting tube 131 through a light-collecting tube, thereby connecting to the syringe 110. The second switching element 123 is used to control the on / off state of the sampling tube 122. Optionally, the second switching element 123 is a valve or other component capable of on / off control to switch the connection relationship between the sampling tube 122 and the first connecting tube 131.

[0080] Specifically, when the sample analyzer 100 needs to aspirate a sample, the second switching element 123 controls the sampling tubing 122 to connect with the first connecting tubing 131, so that the sampling needle 121 is connected with the syringe 110. At this time, the syringe 110 can control the sampling needle 121 to aspirate the sample to be tested from the test tube, completing the sample aspiration operation. When the sample analyzer 100 needs to dispense a sample, the second switching element 123 controls the sampling tubing 122 to connect with the first connecting tubing 131, so that the sampling needle 121 is connected with the syringe 110. At this time, the syringe 110 can control the sampling needle 121 to dispense the sample to be tested from the test tube, completing the sample dispensing operation. When the sample analyzer 100 performs sample pushing or other operations, the second switching element 123 causes the sampling assembly 120 to form an open circuit, and the syringe 110 cannot perform sample aspiration and dispensing operations.

[0081] Furthermore, the movement of the sampling needle 121 is driven by the sampling drive unit. Specifically, the sampling needle 121 is connected to the sampling drive unit, which controls the movement of the sampling needle 121, causing the sampling needle 121 to move between the sampling position, the RBC reaction assembly, and the optical reaction assembly, and controls the sampling needle 121 to perform lifting and lowering movements, thereby realizing the sampling needle 121's sample aspiration and separation.

[0082] See Figures 3 to 5 In one embodiment, the first connecting conduit 131 further has a second access point, which is connected to the sampling conduit 122, and the second switching element 123 is disposed on the sampling conduit 122. Optionally, the first access point S1 is a tee or a component with three interfaces. Two of the three interfaces of the second access point are connected to the first connecting conduit 131 to form a passage in the first connecting conduit 131, while the third interface of the second connection point is connected to the sampling conduit 122.

[0083] Setting a second access point is equivalent to extending a branch from the first connecting pipe 131, which connects the first connecting pipe 131 to the sampling pipe 122. Thus, the second switching element 123 can be directly installed on the sampling pipe 122. After the second switching element 123 is installed on the sampling pipe 122, it can directly control the on / off state of the sampling pipe 122. When the second switching element 123 controls the sampling pipe 122 to be open, the sampling pipe 122 can connect the sampling needle 121 to the syringe 110; when the second switching element 123 controls the sampling pipe 122 to be closed, the sampling pipe 122 cannot connect the sampling needle 121 to the syringe 110.

[0084] Optionally, the second switching element 123 is a two-way valve. Further, the second switching element 123 is a normally open two-way valve or a normally closed two-way valve.

[0085] See Figure 1 and Figure 2In one embodiment, the second switching element 123 is disposed at the connection between the sampling pipeline 122 and the first connecting pipeline 131. That is, the second switching element 123 is located at the bifurcation point between the sampling pipeline 122 and the first connecting pipeline 131. In this case, the second switching element 123 has three leads, two of which are connected to the first connecting pipeline 131, forming a passage in the first connecting pipeline 131, and the third lead of the second switching element 123 extends out and connects to the sampling pipeline 122. The second switching element 123 establishes the connection between the sampling pipeline 122 and the first connecting pipeline 131, thereby controlling the on / off state of the first connecting pipeline 131 and the sampling pipeline 122. Exemplarily, the second switching element 123 is a three-way valve.

[0086] By cooperating with the first switching element 134 and the second switching element 123, the syringe 110 can be connected to only one component through the first connecting tube 131. The first switching element 134 and the second switching element 123 can be a combination of a three-way valve and / or a two-way valve.

[0087] In the first embodiment of the present invention, as Figure 1 As shown, both the first switching element 134 and the second switching element 123 are three-way valves. The second switching element 123 is located between the first connecting pipe 131 and the sampling pipe 122. The first switching element 134 is connected to the first connecting pipe 131 and the second connecting pipe 132 through three leads.

[0088] Specifically, when the sample analyzer 100 aspirates or dispenses a sample, the main controller controls the second switching element 123 to connect the sampling tube 122 and the first connecting tube 131, and controls the first connecting tube 131 to form an open circuit with the second switching element 123. At this time, the syringe 110 is disconnected from the second connecting tube 132 and the third connecting tube 133, and the syringe 110 is connected to the sampling needle 121. The syringe 110 can control the sampling needle 121 to perform the aspiration or dispensing operation of the sample to be tested. When the sample analyzer 100 performs the pushing operation, the main controller controls the second switching element 123 to connect the first connecting tube 131 and the second switching element 123, and disconnects the sampling tube 122 from the first connecting tube 131. At this time, the main controller can control the second connecting tube 132 or the third connecting tube 133 to connect with the first connecting tube 131 through the first switching element 134, so that the syringe 110 can perform the pushing operation.

[0089] In the second embodiment of the present invention, see Figure 2The first switching component 134 includes a first sub-switching component 1341 and a second sub-switching component 1342. The first sub-switching component 1341 and the second sub-switching component 1342 are two-way valves, and the second switching component 123 is a three-way valve. The second switching component 123 is disposed between the first connecting pipe 131 and the sampling pipe 122. The second connecting pipe 132 and the third connecting pipe 133 are connected to the first connecting pipe 131 through the first access point S1. The first sub-switching component 1341 is disposed in the second connecting pipe 132, and the second sub-switching component 1342 is disposed in the third connecting pipe 133.

[0090] Specifically, when the sample analyzer 100 aspirates or dispenses samples, the main controller controls the second switching element 123 to connect the sampling tube 122 and the first connecting tube 131, and controls the first connecting tube 131 to form an open circuit with the second switching element 123. At this time, the syringe 110 is disconnected from the second connecting tube 132 and the third connecting tube 133, and the syringe 110 is connected to the sampling needle 121. The syringe 110 can control the sampling needle 121 to perform the aspiration or dispensing operation of the sample to be tested. When the sample analyzer 100 performs the pushing operation, the main controller controls the second switching element 123 to connect the first connecting tube 131 and the second switching element 123, and disconnects the sampling tube 122 from the first connecting tube 131. At this time, the main controller can control the second connecting tube 132 or the third connecting tube 133 to connect with the first connecting tube 131 through the first sub-switching element 1341 or the second sub-switching element 1342, so that the syringe 110 can perform the pushing operation.

[0091] In the third embodiment of the present invention, except for the change in the location and type of the second switching element 123, everything else is the same as in the second embodiment. See also Figure 3 The second switching element 123 is a two-way valve. The sampling pipeline 122 is connected to the first connecting pipeline 131 through the second access point. The second switching element 123 is installed on the sampling pipeline 122 and directly controls the opening and closing of the sampling pipeline 122. Furthermore, the second switching element 123 is a normally closed two-way valve.

[0092] Specifically, when the sample analyzer 100 aspirates or dispenses samples, the main controller controls the second switching element 123 to connect the sampling tube 122 to the first connecting tube 131, and controls the first sub-switching element 1341 and the second sub-switching element 1342 to disconnect the first connecting tube 131 from the second connecting tube 132 and the third connecting tube 133. At this time, the syringe 110 is disconnected from the second connecting tube 132 and the third connecting tube 133, and the syringe 110 is connected to the sampling needle 121. The syringe 110 can control the sampling needle 121 to perform the aspiration or dispensing operation of the sample to be tested. When the sample analyzer 100 performs the pushing operation, the second switching element 123 does not move and can directly disconnect the sampling tube 122. At this time, the main controller can control the second connecting tube 132 or the third connecting tube 133 to connect with the first connecting tube 131 through the first sub-switching element 1341 or the second sub-switching element 1342, so that the syringe 110 can perform the pushing operation.

[0093] In the fourth embodiment of the present invention, see Figure 4 Except for the second switching component 123 being a normally open two-way valve, the connection relationship and working mode of the sample analyzer 100 are exactly the same as those in the third embodiment, and will not be described in detail here.

[0094] In the fifth embodiment of the present invention, except for the change in the location and type of the second switching element 123, everything else is the same as in the first embodiment. See also Figure 5 The second switching element 123 is a two-way valve. The sampling pipeline 122 is connected to the first connecting pipeline 131 through the second access point. The second switching element 123 is set on the sampling pipeline 122 and directly controls the on / off state of the sampling pipeline 122. The first switching element 134 is a three-way valve.

[0095] Specifically, when the sample analyzer 100 aspirates or dispenses samples, the main controller controls the second switching element 123 to connect the sampling tube 122 to the first connecting tube 131, and controls the first switching element 134 to disconnect the first connecting tube 131 from the second connecting tube 132 and the third connecting tube 133. At this time, the syringe 110 is disconnected from the second connecting tube 132 and the third connecting tube 133, and the syringe 110 is connected to the sampling needle 121. When the sample analyzer 100 performs a sample pushing operation, the main controller controls the second switching element 123 to disconnect the first connecting tube 131 from the sampling tube 122. At this time, the main controller can control the second connecting tube 132 or the third connecting tube 133 to connect with the first connecting tube 131 through the first switching element 134, so that the syringe 110 can perform a sample pushing operation.

[0096] It is worth noting that in the above embodiments, when the second switching component 123 adopts a three-way valve, the sampling component 120 requires that the second connecting pipe 132 or the third connecting pipe 133 connected to the normally open end of the second switching component 123 be a closed pipe to avoid interference with the sampling.

[0097] See Figures 1 to 5 In one embodiment, the second connecting conduit 132 has a third access point S3, through which the second connecting conduit 132 is connected to the sheath current impedance detection component 150 and the RBC reaction component, respectively. Optionally, the third access point S3 is a tee or a component with three interfaces. The third access point S3 establishes a connection between the second connecting conduit 132 and the RBC reaction component and the sheath current impedance detection component 150. The third access point S3 has three interfaces, one of which is connected to the second connecting conduit 132, and the other two interfaces are connected to the RBC reaction component and the sheath current impedance detection component 150, respectively.

[0098] Setting a third connection point is equivalent to creating two branches on the second connection tube 132, forming a Y-shaped pathway and connecting the second connection tube 132 to the RBC reaction assembly and the sheath flow impedance detection assembly 150. After the RBC reaction assembly incubates the sample to be tested to form the first sample solution, the sheath flow impedance detection assembly 150 can use negative pressure to draw the first sample solution out of the RBC reaction assembly. Subsequently, it is connected to the syringe 110 through the second connection tube 132, so that the syringe 110 can quantitatively push the first sample solution into the sheath flow impedance detection assembly 150 to realize the detection of the sample to be tested.

[0099] In one embodiment, the RBC reaction assembly includes an RBC reaction chamber 140, and the piping assembly 130 further includes an RBC sample preparation line 135 and a third switching element 136. The RBC reaction chamber 140 is used to process the sample to be tested to form a first sample solution. The RBC sample preparation line 135 is connected to a third access point S3 and the RBC reaction chamber 140, and the third switching element 136 is disposed on the RBC sample preparation line 135. The RBC reaction chamber 140 is used to incubate the sample to be tested to form the first sample solution. Specifically, a first reagent supply unit is connected to the RBC reaction chamber 140 and is used to deliver processing reagents to the RBC reaction chamber 140 so that the processing reagents process and incubate the sample to be tested in the RBC reaction chamber 140 to form the first sample solution. Optionally, the third switching element 136 is a valve or other component capable of on / off control.

[0100] When the sample to be tested is incubated in the RBC reaction chamber 140, the third switching element 136 is in the off state, and the RBC sample preparation line 135, the second connecting line 132, and the sheath flow impedance detection component 150 are all disconnected. After the sample to be tested in the RBC reaction chamber 140 has finished incubating, the third switching element 136 is opened, and the RBC sample preparation line 135, the second connecting line 132, and the sheath flow impedance detection component 150 are connected, allowing the first sample solution in the RBC reaction chamber 140 to be output. At this time, the sheath flow impedance detection component 150 outputs negative pressure to draw out the first sample solution until it is separated from the RBC sample preparation line 135. However, the first sample solution cannot directly enter the sheath flow impedance detection component 150 for detection. This requires the second connecting line 132 to connect to the syringe 110, and the syringe 110 pushes the first sample solution into the sheath flow impedance detection component 150 to realize the detection of the sample to be tested.

[0101] Understandably, the sheath flow impedance detection component 150 has a first preparation section 1541, which connects the sheath flow impedance detection component 150 to the third access point S3. The first sample liquid aspirated by the sheath flow impedance detection component 150 is stored in the first preparation section 1541, but cannot yet enter the sheath flow impedance detection component 150. Power is provided by the syringe 110 to push the first sample liquid into the sheath flow impedance detection component 150. Furthermore, the syringe 110 can control the volume of the first sample liquid used for sheath flow impedance detection, ensuring a quantitative volume for detection and guaranteeing detection accuracy.

[0102] The sheath flow impedance detection assembly 150 is used to detect a first sample solution prepared from a portion of a sample to be tested and red blood cell reagent supplied from a first reagent supply unit to obtain red blood cell parameters and platelet parameters. In one embodiment, the sheath flow impedance detection assembly 150 includes a sheath flow impedance detection sample preparation power source 153, a sheath flow impedance detection unit 151, a sheath flow impedance detection sample needle 152, a sheath flow impedance detection sample preparation line 154, and a fourth switching element 156. The sheath flow impedance detection sample preparation line 154 has a fourth access point S4, is connected to the sheath flow impedance detection sample preparation power source 153, and is connected to a third access point S3 to connect to the RBC sample preparation line 135. The sheath flow impedance detection sample needle 152 is connected to the fourth access point S4 and is located in the sheath flow impedance detection unit 151, which is connected to the sheath fluid chamber 155. The power source 153 for preparing the sample for sheath resistance detection draws the first sample liquid from the RBC reaction cell 140 into the sample preparation tubing 154 and the needle 152 for sheath resistance detection, and the syringe 110 pushes the first sample liquid into the sheath resistance detection section 151.

[0103] The sheath flow impedance detection unit 151 has a detection port with electrodes and an outlet for outputting the first sample liquid after detection. The sheath flow impedance detection unit 151 detects the DC impedance generated when particles in the first sample liquid pass through the detection port and outputs an electrical signal reflecting information about the particles passing through the port. Exemplarily, the sheath flow impedance detection unit 151 is a sheath flow impedance counting cell. The sheath flow impedance detection sample preparation line 154 is used to transport the first sample liquid to the sheath flow impedance detection unit 151. The sheath fluid chamber 155 is connected to the sheath flow impedance detection unit 151 and is used to supply sheath fluid to the sheath flow impedance detection unit 151.

[0104] The sheath flow impedance detection sample preparation pipeline 154 includes two sections: a first preparation liquid section 1541 and a first negative pressure power source connection pipeline 1542. One end of the first preparation liquid section 1541 is connected to a third access point S3, and the other end of the first preparation liquid section 1541 is connected to the sheath flow impedance detection sample needle 152 and the first negative pressure power source connection pipeline 1542 via a fourth access point S4. The end of the sheath flow impedance detection sample needle 152 extends into the sheath flow impedance detection section 151. The end of the first negative pressure power source connection pipeline 1542 away from the fourth access point S4 is connected to the sheath flow impedance detection sample preparation power source 153. The sheath flow impedance detection sample preparation power source 153 can generate negative pressure to draw the first sample liquid from the RBC reaction cell 140. Furthermore, a fourth switching element 156 is disposed in the first negative pressure power source connection pipeline 1542 to control the on / off state of the first negative pressure power source connection pipeline 1542.

[0105] After the sample to be tested in the RBC reaction chamber 140 has completed incubation and formed the first sample solution, the main controller controls the sheath flow impedance detection sample preparation power source 153 to prepare, and controls the third switching element 136 and the fourth switching element 156 to open, so that the first negative pressure power source connecting pipe 1542, the first preparation liquid section 1541 and the RBC sample preparation pipe 135 are connected. At this time, the sheath flow impedance detection sample preparation power source 153 generates negative pressure, which draws the first sample solution in the RBC reaction chamber 140 into the first preparation liquid section 1541, which is located at the fourth access point S4, that is, the first sample solution is drawn into the inlet of the sheath flow impedance detection section 151. Subsequently, the main controller controls the first switching element 134 and the second switching element 123 to operate, so that the second connecting pipe 132 is connected to the syringe 110 through the first connecting pipe 131. The syringe 110 starts to push the sample, so that the first sample solution in the first preparation liquid section 1541 is sprayed into the sheath flow impedance detection section 151 through the sheath flow impedance detection sample needle 152.

[0106] Inside the sheath flow impedance detection unit 151, when the sheath flow impedance detection sample needle 152 ejects the first sample liquid, the first sample liquid flows under the influence of the sheath fluid. The detection orifice causes the first sample liquid flow to become a thin stream, allowing the particles (formed elements) contained in the first sample liquid to pass through the detection orifice one by one. The electrodes are electrically connected to a DC power supply, which provides DC current between a pair of electrodes. During the DC current supply, the impedance between the pair of electrodes can be detected. The resistance signal, representing the impedance change, is amplified by an amplifier and then sent to the main controller. The magnitude of the resistance signal corresponds to the volume (size) of the particles; therefore, by processing the resistance signal through the main controller, the red blood cell parameters and platelet parameters of the sample liquid to be tested can be obtained.

[0107] It is worth noting that the sheath current impedance detection unit 151 is the sheath current impedance counting cell, and its specific structure and detection principle are existing technologies. In this invention, for ease of understanding, only the detection technology of the sheath current impedance detection unit 151 is described.

[0108] See Figures 1 to 5 In one embodiment, the third connecting conduit 133 has a fifth access point S5, through which the optical detection component 170 and the optical reaction component are connected. Optionally, the fifth access point S5 is a tee or a component with three interfaces. The fifth access point S5 establishes a connection between the third connecting conduit 133 and the optical reaction component and optical detection component 170. The fifth access point S5 has three interfaces, one of which is connected to the third connecting conduit 133, and the other two are connected to the optical reaction component and optical detection component 170, respectively.

[0109] Setting a third connection point is equivalent to forming two branches on the third connection tube 133, creating a Y-shaped pathway and establishing communication between the third connection tube 133 and the optical reaction assembly and optical detection assembly 170. After the optical reaction assembly incubates the sample to be tested to form a second sample solution, the optical detection assembly 170 can use negative pressure to draw the second sample solution out of the optical reaction assembly. Subsequently, it is connected to the syringe 110 through the third connection tube 133, allowing the syringe 110 to quantitatively push the second sample solution into the optical detection assembly 170, thus achieving the detection of the sample to be tested.

[0110] In one embodiment, the optical reaction assembly includes an optical reaction cell 160; the piping assembly 130 further includes an optical sample preparation piping 137 and a fifth switching element 138. The optical reaction cell 160 is used to process the sample to be tested to form a second sample solution. The optical sample preparation piping 137 is connected to the optical reaction cell 160 via a fifth access point S5, and the fifth switching element 138 is disposed on the optical sample preparation piping 137. The optical reaction cell 160 is used to incubate the sample to be tested to form the second sample solution. Specifically, a second reagent supply unit is connected to the optical reaction cell 160 and is used to deliver processing reagents to the optical reaction cell 160 so that the processing reagents process and incubate the sample to be tested in the optical reaction cell 160 to form the second sample solution. Optionally, the fifth switching element 138 is a valve or other component capable of on / off control.

[0111] When the sample to be tested is incubated in the optical reaction chamber 160, the fifth switching element 138 is in the off state, and the optical sample preparation pipeline 137, the third connecting pipeline 133, and the optical detection component 170 are all disconnected. After the sample to be tested in the optical reaction chamber 160 has finished incubating, the fifth switching element 138 is opened, and the optical sample preparation pipeline 137, the third connecting pipeline 133, and the optical detection component 170 are connected, allowing the second sample liquid in the optical reaction chamber 160 to be output. At this time, the optical detection component 170 outputs negative pressure to draw out the second sample liquid until it is separated from the optical sample preparation pipeline 137. However, the second sample liquid cannot directly enter the optical detection component 170 for detection. This requires the third connecting pipeline 133 to connect to the syringe 110, and the syringe 110 pushes the second sample liquid into the optical detection component 170 to realize the detection of the sample to be tested.

[0112] Understandably, the optical detection component 170 has a second liquid preparation section 1741, which connects the optical detection component 170 to the fifth access point S5. The second sample liquid aspirated by the optical detection component 170 is stored in the second liquid preparation section 1741, but cannot yet enter the optical detection component 170. Power is provided by the syringe 110 to push the second sample liquid into the optical detection component 170. Furthermore, the syringe 110 can control the volume of the second sample liquid used for optical detection, ensuring a quantitative volume for detection and guaranteeing detection accuracy.

[0113] The optical detection component 170 is used to detect a second sample solution prepared from a portion of the sample to be tested and a leukocyte reagent supplied from the second reagent supply unit to obtain optical parameters of the sample to be tested. Exemplarily, the optical detection component can detect WBCs (white blood cells), NRBCs (nucleated red blood cells), RETs (reticulocytes), BRCs, PLTs, etc., in the sample to be tested.

[0114] In one embodiment, the optical detection assembly 170 includes an optical sample preparation power source 173, an optical detection unit 171, an optical sample needle 172, an optical detection tubing 174, and a sixth switching element 175. The optical detection tubing 174 has a sixth access point S6, is connected to the optical sample preparation power source 173, and is connected to a fifth access point S5 to connect to the optical sample preparation tubing 137. The optical sample needle 172 is connected to the sixth access point S6 and extends into the optical detection unit 171. The optical sample preparation power source 173 draws the second sample liquid from the optical reaction cell 160 into the optical detection tubing 174 and the optical sample needle 172, and the syringe 110 pushes the second sample liquid into the optical detection unit 171.

[0115] The optical detection conduit 174 includes two sections: a second preparation liquid section 1741 and a second negative pressure power source connection conduit 1742. One end of the second preparation liquid section 1741 is connected to the fifth access point S5, and the other end of the second preparation liquid section 1741 is connected to the optical detection sample needle 172 and the second negative pressure power source connection conduit 1742 via the sixth access point S6. The end of the optical detection sample needle 172 extends into the optical detection unit 171. The end of the second negative pressure power source connection conduit 1742 away from the sixth access point S6 is connected to the optical detection sample preparation power source 173. The optical detection sample preparation power source 173 can generate negative pressure to draw the second sample liquid from the optical reaction cell 160. Furthermore, a sixth switching element 175 is disposed in the second negative pressure power source connection conduit 1742 to control the on / off state of the second negative pressure power source connection conduit 1742.

[0116] After the sample to be tested in the optical reaction cell 160 has completed incubation and formed the second sample solution, the main controller controls the optical detection sample preparation power source 173 to prepare, and controls the fifth switching element 138 and the sixth switching element 175 to open, so that the second negative pressure power source connecting pipe 1742, the second preparation liquid section 1741, and the optical sample preparation pipe 137 are connected. At this time, the optical detection sample preparation power source 173 generates negative pressure, which draws the second sample solution in the optical reaction cell 160 into the second preparation liquid section 1741, which is located at the sixth access point S6, that is, the second sample solution is drawn into the inlet of the optical detection unit 171. Subsequently, the main controller controls the first switching element 134 and the second switching element 123 to operate, so that the third connecting pipe 133 is connected to the syringe 110 through the first connecting pipe 131. The syringe 110 begins to push the sample, so that the second sample solution in the second preparation liquid section 1741 is sprayed into the optical detection unit 171 through the optical detection sample needle 172.

[0117] The optical detection unit 171 includes a light source, a beam shaping unit, a flow chamber, and a forward-scattering light detector arranged sequentially in a straight line. A dichroic mirror is arranged at a 45° angle to the line on one side of the flow chamber. After the optical detection sample needle 172 ejects the second sample liquid, a portion of the side light emitted by the blood cells in the flow chamber passes through the dichroic mirror and is captured by a fluorescence detector arranged at a 45° angle behind the dichroic mirror, while the other portion of the side light is reflected by the dichroic mirror and captured by a side-scattering light detector arranged at a 45° angle in front of the dichroic mirror. Based on the forward-scattering light signal captured by the forward-scattering light detector, the side-scattering light signal captured by the side-scattering light detector, and the fluorescence signal captured by the fluorescence detector, white blood cells in the blood sample can be counted and classified; for example, white blood cells can be classified into at least neutrophils, lymphocytes, and monocytes.

[0118] It is worth noting that the optical detection unit 171 is the optical flow chamber, and its specific structure and detection principle are existing technologies. In this invention, only the detection technology of the optical detection unit 171 is described for ease of understanding.

[0119] In one embodiment, the syringe 110 has a volume range of 100 μL to 300 μL. That is, the syringe 110 of the present invention has a relatively large volume range. This satisfies the quantitative accuracy and volume requirements of each module, enabling the syringe 110 to be reused and allowing one syringe 110 to work with various modules. Preferably, the syringe 110 has a volume range of 200 μL to 300 μL. Further, the syringe 110 has a volume range of 250 μL. Furthermore, to improve the quantitative accuracy of the syringe 110, an injection drive unit is used to achieve precise control of the syringe 110, ensuring accurate detection results. Optionally, the injection drive unit is a stepper motor.

[0120] See Figures 1 to 5 In one embodiment, the sample analyzer 100 further includes a diluent assembly 180, which is connected to a syringe 110. The diluent assembly 180 is used to provide diluent to various modules of the sample analyzer 100. For example, when the RBC reaction assembly requires diluent, the syringe 110 can control the delivery of diluent from the diluent assembly 180 into the RBC reaction assembly; when cleaning is required, the syringe 110 delivers diluent from the diluent assembly 180 into the components that need cleaning.

[0121] In one embodiment, the diluent assembly 180 includes a diluent line 182, a diluent chamber 181, and a seventh switching element 183. The diluent line 182 connects the syringe 110 and the diluent chamber 181, and the seventh switching element 183 is disposed on the diluent line 182. The seventh switching element 183 is a valve or other component capable of on / off control. The seventh switching element 183 controls the on / off state of the diluent line 182 to control the on / off state of the syringe 110 and the diluent chamber 181. When diluent is needed, the seventh switching element 183 controls the diluent line 182 to open, connecting the syringe 110 and the diluent chamber 181. When diluent is not needed, the seventh switching element 183 controls the diluent line 182 to close, disconnecting the syringe 110 from the diluent chamber 181.

[0122] The operation of the sample analyzer 100 of the present invention is described using the arrangement of the switching components in the first embodiment. The working principles of the switching components in other embodiments are essentially the same as those in the first embodiment, and will not be described in detail here. See also Figure 1 The working process of the sample analyzer 100 in the first embodiment is as follows:

[0123] When sampling component 120 samples, it first moves to the sampling position. The main controller controls the second switching element 123 to connect the sampling tube 122 and the first connecting tube 131, so that the sampling needle 121 is connected to the syringe 110. At the same time, the main controller controls the first switching element 134 to disconnect the first connecting tube 131 from the second switching element 123, so that the second switching element 123 disconnects the connection between the first connecting tube 131 and the second connecting tube 132 and the third connecting tube 133. At this time, the main controller controls the syringe 110 to drive the sampling needle 121 to sample. After sampling, the main controller controls the sampling component 120 to move to the RBC reaction component and dispense the sample into the RBC reaction cell 140. The RBC reaction cell 140 incubates the sample to be tested to form the first sample solution. During the incubation process, the main controller controls the sampling component 120 to move to the optical reaction component and dispense the sample into the optical reaction cell 160. The optical reaction cell 160 incubates the sample to be tested to form the second sample solution. The main controller controls the second switching element 123 to shut down the sampling pipeline 122.

[0124] After the sampling component 120 completes sample dispensing, the sheath flow impedance detection component 150 performs sample preparation. The sheath flow impedance detection sample preparation power source 153 operates, and the main controller controls the third switching element 136 and the fourth switching element 156 to open. The negative pressure of the sheath flow impedance detection sample preparation power source 153 draws the first sample liquid in the RBC reaction cell 140 into the first preparation section 1541, and draws the first sample liquid into the fourth access point S4, so that the first sample liquid is located at the inlet of the sheath flow impedance detection section 151. Then, the sheath flow impedance detection component 150 prepares for measurement. The main controller controls the first switching element 134 to connect the second switching element 123 and the first connecting pipe 131, and controls the second switching element 123 to open the second connecting pipe 132, so that the first switching element 134 and the second switching element 123 connect the first connecting pipe 131 and the second connecting pipe 132, making the syringe 110 connected to the sheath flow impedance detection section 151. The syringe 110 begins to push the sample into the sheath flow resistance detection unit 151, which then detects the first sample solution.

[0125] After the syringe 110 pushes the first sample liquid, the optical detection assembly 170 performs sample preparation. The optical detection sample preparation power source 173 operates, and the main controller controls the fifth switching element 138 and the sixth switching element 175 to open. The negative pressure of the optical detection sample preparation power source 173 draws the second sample liquid in the optical reaction cell 160 into the second preparation section 1741, and draws the second sample liquid into the sixth access point S6, so that the second sample liquid is located at the entrance of the optical detection unit 171. Then, the optical detection assembly 170 prepares for measurement. The main controller controls the first switching element 134 to connect the second switching element 123 with the first connecting pipe 131, and controls the second switching element 123 to open the third connecting pipe 133, so that the first switching element 134 and the second switching element 123 connect the first connecting pipe 131 and the third connecting pipe 133, making the syringe 110 and the optical detection unit 171 connected. The syringe 110 begins to push the sample into the optical detection unit 171, and the optical detection unit 171 detects the second sample liquid.

[0126] After optical detection and sheath flow impedance detection are completed, the main controller controls the seventh switching element 183 to open, using diluent to clean all components and prevent cross-contamination. After cleaning, the main controller controls the syringe 110 to reset. At this point, the sample analyzer 100 stops working or begins the next measurement cycle.

[0127] See Figures 1 to 5 The present invention also provides a detection method for a sample analyzer 100, comprising the following steps:

[0128] The sampling component 120 is moved above the sample to be tested, and the syringe 110 is controlled to drive the sampling component 120 to collect the sample to be tested;

[0129] The sampling component 120 is moved to the RBC reaction component, and the syringe 110 is controlled to drive the sampling component 120 to deliver the sample to be tested into the RBC reaction component. The RBC reaction component processes the sample to be tested to form a first sample solution.

[0130] The sampling component 120 is moved to the optical reaction component, and the syringe 110 is controlled to drive the sampling component 120 to deliver the sample to be tested into the optical reaction component. The optical reaction component processes the sample to be tested to form a second sample solution.

[0131] The first sample solution in the RBC reaction assembly is allowed to flow, and the first sample solution is pushed into the sheath flow impedance detection assembly 150 by the syringe 110;

[0132] The second sample liquid in the optical reaction assembly is allowed to flow, and the syringe 110 pushes the second sample liquid into the optical detection assembly 170;

[0133] Within one measurement cycle, the syringe 110 continuously works in conjunction with the sampling component 120, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component 150, and the optical detection component 170.

[0134] The sample analyzer 100 of this invention operates continuously in conjunction with a sampling component 120, an RBC reaction component, a sheath current impedance detection component 150, and an optical detection component 170 via a syringe 110. One measurement cycle of the sample analyzer 100 is as follows: the sampling component 120 moves to the sampling position, and the syringe 110 controls the sampling component 120 to aspirate the sample; subsequently, the sampling component 120 moves to the RBC reaction component, and the syringe 110 controls the sampling component 120 to dispense the sample into the RBC reaction component, which processes the sample to form a first sample solution; the sampling component 120 then moves to the optical reaction component, and the syringe 110 controls the sampling component 120 to dispense the sample into the optical reaction component, which processes the sample to form a second sample solution. Then, the first sample solution flows out of the RBC reaction component, and the syringe 110 pushes the first sample solution into the sheath current impedance detection component 150; the second sample solution flows out of the optical reaction component, and the syringe 110 pushes the second sample solution into the optical detection component 170, completing the detection of one sample.

[0135] The syringe 110 operates continuously with each component within a measurement cycle; that is, the syringe 110 performs sample aspiration, dispensing, and dispensing actions sequentially. After completing the sample aspiration operation, the syringe 110 performs the dispensing operation, followed by the dispensing operation. Understandably, because the movement of the sampling component 120 and the transfer of the sample liquid require a certain amount of time, the syringe 110 remains stationary during this process. The syringe 110 operates only when the sampling component 120, RBC reaction component, optical reaction component, sheath current impedance detection component 150, and optical detection component 170 require operation.

[0136] In the above description, although expressions such as "first" and "second" may be used to describe various elements of the invention, they are not intended to limit the corresponding elements. For example, the above expressions are not intended to limit the order or importance of corresponding elements. The above expressions are used to distinguish one component from another.

[0137] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0138] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A sample analyzer, characterized in that, It includes a sheath flow impedance detection assembly, an RBC reaction assembly, a tubing assembly, an optical reaction assembly, an optical detection assembly, a sampling assembly, and a syringe; The sampling component is used to collect the sample to be tested; the RBC reaction component is used to process the sample to be tested to form a first sample solution; the sheath current impedance detection component is used to detect the first sample solution; the optical reaction component is used to process the sample to be tested to form a second sample solution; and the optical detection component is used to detect the second sample solution. The syringe is connected to the sheath flow impedance detection component, the RBC reaction component, the optical reaction component, the optical detection component, and the sampling component through the tubing assembly, and works continuously in conjunction with the sampling component, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component, and the optical detection component.

2. The sample analyzer according to claim 1, characterized in that, During continuous operation, the syringe can sample and separate the sample to be tested. The syringe can also push the first sample liquid into the sheath flow impedance detection component and push the second sample liquid into the optical detection component.

3. The sample analyzer according to claim 2, characterized in that, The tubing assembly includes a first connecting tubing, a second connecting tubing, and a third connecting tubing. The first connecting tubing connects the sampling component and the syringe. One end of the second connecting tubing is connected to the first connecting tubing, and the other end of the second connecting tubing is connected to the sheath current impedance detection component. One end of the third connecting tubing is connected to the first connecting tubing, and the other end of the third connecting tubing is connected to the optical detection component.

4. The sample analyzer according to claim 3, characterized in that, The pipeline assembly further includes a first switching component, which enables the connection and disconnection of the first connecting pipeline with the second connecting pipeline and the third connecting pipeline.

5. The sample analyzer according to claim 4, characterized in that, The first switching component is disposed at the connection between the first connecting pipe and the second connecting pipe and the third connecting pipe. The first switching component has three leads, one of which is connected to the first connecting pipe, and the other two leads are respectively connected to the first connecting pipe and the second connecting pipe.

6. The sample analyzer according to claim 4, characterized in that, The first connecting pipe has a first access point, which is connected to the second connecting pipe and the third connecting pipe respectively; the first switching component includes a first sub-switching component and a second sub-switching component, the first sub-switching component is disposed in the second connecting pipe, and the second sub-switching component is disposed in the third connecting pipe.

7. The sample analyzer according to any one of claims 3 to 6, characterized in that, The sampling assembly includes a sampling needle, a sampling tubing, and a second switching element. The sampling tubing connects the sampling needle and the second switching element. The first connecting tubing connects the second switching element and the syringe. The syringe and the second switching element work together to sample and separate the sample to be tested.

8. The sample analyzer according to claim 7, characterized in that, The first connecting pipe also has a second access point, which is connected to the sampling pipe, and the second switching element is disposed in the sampling pipe.

9. The sample analyzer according to claim 7, characterized in that, The second switching element is disposed at the connection between the sampling pipeline and the first connecting pipeline.

10. The sample analyzer according to any one of claims 3 to 6, characterized in that, The second connecting pipe has a third access point, through which the second connecting pipe is connected to the sheath current impedance detection component and the RBC reaction component respectively.

11. The sample analyzer according to claim 10, characterized in that, The RBC reaction assembly includes an RBC reaction chamber, and the pipeline assembly further includes an RBC sample preparation pipeline and a third switching element. The RBC reaction chamber is used to process the sample to be tested to form a first sample solution. The RBC sample preparation pipeline connects the third access point to the RBC reaction chamber, and the third switching element is disposed on the RBC sample preparation pipeline.

12. The sample analyzer according to any one of claims 3 to 6, characterized in that, The third connecting pipe has a fifth access point, and the third connecting pipe connects the optical detection component and the optical reaction component through the fifth access point.

13. The sample analyzer according to claim 12, characterized in that, The optical reaction assembly includes an optical reaction cell; the pipeline assembly further includes an optical sample preparation pipeline and a fifth switching element. The optical reaction cell is used to process the sample to be tested to form a second sample liquid. The optical sample preparation pipeline connects the fifth access point to the optical reaction cell, and the fifth switching element is disposed on the optical sample preparation pipeline.

14. The sample analyzer according to any one of claims 1 to 6, characterized in that, The syringe has a volume range of 100uL to 300uL.

15. A detection method for a sample analyzer, characterized in that, Includes the following steps: The sampling component is moved above the sample to be tested, and the syringe is controlled to drive the sampling component to collect the sample. The sampling component is controlled to move to the RBC reaction component, and the syringe is controlled to drive the sampling component to deliver the sample to be tested into the RBC reaction component, where the RBC reaction component processes the sample to be tested to form a first sample solution. The sampling component is controlled to move to the optical reaction component, and the syringe is controlled to drive the sampling component to deliver the sample to be tested into the optical reaction component, whereby the optical reaction component processes the sample to be tested to form a second sample solution. The first sample solution in the RBC reaction assembly is allowed to flow, and the syringe pushes the first sample solution into the sheath flow impedance detection assembly; The second sample liquid in the optical reaction assembly is allowed to flow, and the syringe pushes the second sample liquid into the optical detection assembly; Within one measurement cycle, the syringe continuously works in conjunction with the sampling component, the RBC reaction component, the optical reaction component, the sheath flow impedance detection component, and the optical detection component.

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