A tissue dissociation device

Abstract

The present invention discloses a tissue dissociation device, belonging to the technical field of tissue dissociation. The tissue dissociation device includes a sample bottle and a grinding and filtering structure. The grinding and filtering structure includes a grinding member and a filter screen. The grinding end of the grinding member and the filter screen can enter the placement space of the sample bottle through the placement opening of the sample bottle, and divide the placement space into a tissue accommodation area and a cell suspension accommodation area. The grinding end has a sealing position abutting against the filter screen and a filtering position separated from the filter screen. The grinding end in the sealing position can rotate and grind the biological tissue located in the tissue accommodation area. At this time, the cell suspension accommodation area is in a sealed state. When the grinding is completed and the grinding end moves to the filtering position, the cell suspension accommodation area is in a negative pressure state. The cell suspension formed by grinding the biological tissue can be filtered through the filter screen and enter the cell suspension accommodation area for temporary storage. The tissue dissociation device has a high integration degree and a good filtering effect, and can form a high-quality cell suspension.

Description

Technical Field

[0001] The present invention relates to the technical field of tissue dissociation, and in particular, to a tissue dissociation device. Background Art

[0002] A tissue dissociation device refers to a device that uses tissue dissociation technology to dissociate biological tissues to obtain a cell suspension with high activity and high quality. To obtain a cell suspension, the tissue dissociation device first needs to break the extracellular matrix to release cells from the extracellular matrix, and then needs to break the connections between cells to obtain the cell suspension.

[0003] There are many existing tissue dissociation methods, such as enzymatic digestion, electrolysis, or grinding, etc. Since grinding can make the tissue be sheared sufficiently, the dissociation effect is good, and it is widely used. The existing devices using grinding as the tissue dissociation method cannot directly filter the cell suspension after dissociation, and can only transfer the cell suspension to an external filtering device for filtration after grinding, which is cumbersome to operate and will affect the activity and quality of the cell suspension during the transfer process.

[0004] Therefore, how to propose a tissue dissociation device integrating grinding and filtration is a technical problem that needs to be solved urgently now. Summary of the Invention

[0005] The purpose of the present invention is to provide a tissue dissociation device, which integrates grinding and filtration, has a high degree of integration, and has a good filtration effect.

[0006] To achieve this purpose, the present invention adopts the following technical solutions:

[0007] A tissue dissociation device, comprising: a sample bottle, the sample bottle having a storage space and a storage opening; a grinding and filtering structure, the grinding and filtering structure including a grinding member and a filter net, the filter net being movably connected to the grinding end of the grinding member, the grinding end and the filter net being able to enter the storage space through the storage opening, and dividing the storage space into a cell suspension containing area on one side of the filter net and a tissue containing area on one side of the grinding end, and the grinding end having a sealing position where it abuts against the filter net to seal the cell suspension containing area and a filtering position where it is separated from the filter net; the grinding end is configured to be able to rotate around a first axis a in the sample bottle when in the sealing position to grind the biological tissue located in the tissue containing area; and the grinding end is configured to be able to move linearly along the first axis a in a direction away from the filter net and move from the sealing position to the filtering position, so that the cell suspension formed by grinding the biological tissue can be filtered through the filter net under negative pressure and enter the cell suspension containing area.

[0008] Preferably, the grinding end is configured to be able to move linearly away from the filter net along the first axis a and move from the filtering position to the limiting position. The grinding end in the limiting position can be in limiting contact with the filter net, so that the grinding end can drive the filter net to move synchronously along the first axis a.

[0009] Preferably, the grinding end includes a grinding rotor, a grinding stator and a fixing member. A rotating shaft hole is provided through the grinding stator along the direction of the first axis a, and a grinding surface is provided on the grinding stator; the grinding rotor includes a rotor shaft and grinding blades. One end of the grinding blade is connected to the middle of the rotor shaft. The first end of the rotor shaft is used to connect the driving component, and the second end of the rotor shaft can movably pass through the rotating shaft hole, so that the other end of the grinding blade contacts the grinding surface of the grinding stator. When the grinding rotor is configured to rotate, the grinding blade can grind the biological tissue located on the grinding surface; a through hole is provided in the middle of the filter net, and the connecting end of the fixing member can pass through the through hole and be connected to the second end of the rotor shaft. And a first limiting structure capable of passing through the through hole and limiting with the grinding stator is provided on the fixing member, and a second limiting structure is provided at the limiting end of the fixing member. The second limiting structure can be limited on the side of the filter net away from the grinding stator.

[0010] Preferably, the fixing member is a stepped shaft, the outer diameter of the stepped shaft is smaller than the size of the through hole. The stepped shaft includes a small-diameter shaft and a large-diameter shaft connected coaxially. One end of the small-diameter shaft away from the large-diameter shaft is connected to the second end of the rotor shaft. The first limiting structure is a first limiting surface formed between the small-diameter shaft and the large-diameter shaft; the rotating shaft hole is a stepped hole, which includes a small-aperture hole and a large-aperture hole connected in sequence. The large-aperture hole is close to the filter net, and a second limiting surface is formed between the small-aperture hole and the large-aperture hole. The first limiting surface can be in limiting contact with the second limiting surface.

[0011] Preferably, the second limiting structure is a limiting boss, the limiting boss is arranged at one end of the large-diameter shaft away from the small-diameter shaft, and the size of the limiting boss is larger than the size of the through hole.

[0012] Preferably, the grinding surface is a conical surface, and a grinding edge is inclined at the other end of the grinding blade. The grinding edge can move on the conical surface to cut the biological tissue.

[0013] Preferably, a plurality of grinding grooves are provided on the grinding surface, and the plurality of grinding grooves rotate around the first axis a.

[0014] Preferably, a positioning step is provided on the inner wall surface of the sample bottle, and the filter net can be placed on the positioning step.

[0015] Preferably, a third limiting structure is provided between the grinding stator and the sample bottle to limit the rotation of the grinding stator around the first axis a.

[0016] Preferably, the third limiting structure includes a limiting block provided on the inner wall surface of the sample bottle and a limiting groove provided on the outer wall surface of the grinding stator, and the limiting block can be inserted into the limiting groove.

[0017] Advantages of the present invention:

[0018] The present invention provides a tissue dissociation device, which includes a sample bottle and a grinding and filtering structure. The grinding and filtering structure includes a grinding member and a filter net movably connected to the grinding end of the grinding member. The grinding end and the filter net can enter the storage space of the sample bottle through the storage opening of the sample bottle, and divide the storage space into a cell suspension accommodation area and a tissue accommodation area. The grinding end has a sealing position abutting against the filter net and a filtering position separated from the filter net. When the grinding end is in the sealing position, the grinding end can rotate and grind the biological tissue located in the tissue accommodation area. At this time, the cell suspension accommodation area is in a sealed state. When the grinding is completed and the grinding end moves to the filtering position, the cell suspension accommodation area is in a negative pressure state. At this time, under the action of atmospheric pressure, the cell suspension formed by grinding the biological tissue can pass through the filter net for filtration and enter the cell suspension accommodation area for temporary storage. This tissue dissociation device integrates the grinding member and the filter net, with a high degree of integration, which is beneficial to improving the production efficiency and activity of the cell suspension. Moreover, the cell suspension is filtered under negative pressure, and the filtering effect is good, which is conducive to forming a high-quality cell suspension. Description of the Drawings

[0019] Figure 1 is a schematic structural diagram of the tissue dissociation device provided by an embodiment of the present invention;

[0020] Figure 2 is a cross-sectional view of the tissue dissociation device provided by an embodiment of the present invention;

[0021] Figure 3 is an exploded view of the tissue dissociation device provided by an embodiment of the present invention;

[0022] Figure 4 is a cross-sectional view of the sample bottle of the tissue dissociation device provided by an embodiment of the present invention;

[0023] Figure 5 is a cross-sectional view of the grinding member of the tissue dissociation device provided by an embodiment of the present invention;

[0024] Figure 6 It is an exploded view of the grinding part of the tissue dissociation device provided by the embodiment of the present invention.

[0025] In the figure:

[0026] 100, sample bottle; 101, storage space; 102, storage opening; 103, positioning step; 104, limiting block;

[0027] 200, grinding and filtering structure; 201, filter net; 202, grinding rotor; 2021, rotor shaft; 2022, grinding blade; 203, grinding stator; 2031, grinding surface; 2032, grinding groove; 2033, rotating shaft hole; 2034, second limiting surface; 204, fixing part; 2041, first limiting surface; 2042, small-diameter shaft; 2043, large-diameter shaft; 2044, limiting boss; 205, bottle cap;

[0028] 300, tissue accommodation area;

[0029] 400, cell suspension accommodation area. Detailed implementation manners

[0030] Next, the technical solutions of the present invention will be described clearly and completely in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0031] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0032] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0033] This embodiment provides a tissue dissociation device with a high degree of integration. It can not only fully grind biological tissues, but also fully filter the ground biological tissues to form a high-quality and highly active cell suspension. Through detecting or testing this cell suspension, scientific researchers can obtain relatively accurate detection data or test data, and thus obtain relatively accurate scientific research results.

[0034] As Figures 1 to 6 shown, this tissue dissociation device includes a sample bottle 100 and a grinding and filtering structure 200. Among them, the sample bottle 100 has a storage space 101 inside, and the storage space 101 penetrates through one end of the sample bottle 100 to form a storage opening 102. Through this storage opening 102, the grinding and filtering structure 200 and biological tissues can be placed in the storage space 101 of the sample bottle 100. In this embodiment, as Figures 1 to 4 shown, the sample bottle 100 is in the shape of a test tube, and its outer shape is cylindrical. This sample bottle 100 can be directly placed on a centrifuge to perform centrifugation on the finally obtained cell suspension. More specifically, the sample bottle 100 includes a straight tube part and a conical part. The storage opening 102 is provided at one end of the straight tube part away from the conical part. The inclined inner wall surface of the conical part makes it easy for the cell suspension to gather at the bottom of the sample bottle 100, avoiding the phenomenon of wall hanging.

[0035] The grinding and filtering structure 200 includes a grinding part and a filter net 201. Among them, the grinding part has two ends, a driving end and a grinding end. The driving end is used to connect an external driving component, and the driving component can be a rotating motor. The grinding end is used to grind biological tissues. The grinding end and the filter net 201 can enter the storage space 101 through the storage opening 102, and divide the storage space 101 into a cell suspension accommodation area 400 on one side of the filter net 201 and a tissue accommodation area 300 on one side of the grinding end. The tissue accommodation area 300 is used to place biological tissues and dissociation solution, and the cell suspension accommodation area 400 is used to accommodate the generated cell suspension. The filter net 201 is movably connected to the grinding end of the grinding part. During the movement of the grinding end, it has a sealing position where it abuts against the filter net 201 to seal the cell suspension accommodation area 400, and a filtering position where it separates from the filter net 201 to form a negative pressure in the cell suspension accommodation area 400.

[0036] When the grinding end moves to the sealing position, the grinding end abuts against the filter net 201, so that the cell suspension accommodation area 400 below the filter net 201 forms a sealed space. And because the biological tissues and dissociation solution in the tissue accommodation area 300 gather at the grinding end, the sealing performance of the cell suspension accommodation area 400 is further improved. At this time, when the grinding part is driven by the driving component, its grinding end can rotate around the first axis a in the sample bottle 100 (as Figure 2It rotates as shown, and the rotating grinding end can grind the biological tissue located in the tissue accommodation area 300. Under the action of mechanical force, the biological tissue is divided and ground, and is dissociated in the dissociation solution, thereby forming a cell suspension doped with tissue fragments. After the grinding is completed, the grinding end can move linearly along the first axis a in a direction away from the filter net 201 under the action of an external force, so that the grinding end moves from the sealed position to the filtering position. At this time, the grinding end is separated from the filter net 201, and the space of the cell suspension accommodation area 400 below the grinding end becomes larger and the pressure becomes smaller, so that it is in a negative pressure state lower than the atmospheric pressure. The cell suspension formed by grinding the biological tissue is filtered through the filter net 201 under the action of negative pressure, and the high-quality and highly active cell suspension after filtration enters the cell suspension accommodation area 400 for temporary storage.

[0037] Compared with the tissue dissociation device that only has a grinding function in the prior art, the tissue dissociation device provided in this embodiment integrates the grinding part and the filter net 201, so that it not only has the dual functions of grinding and filtering, has strong functionality and high integration, but also can filter the cell suspension in the fastest time after grinding is completed, which is beneficial to improving the production efficiency and activity of the cell suspension. Moreover, compared with using gravity to achieve filtration, the tissue dissociation device provided in this embodiment adopts a negative pressure filtration method to filter the cell suspension under the action of negative pressure, and the filtration effect is better, which is conducive to forming a high-quality cell suspension.

[0038] Optionally, after the cell suspension is filtered and enters the cell suspension accommodation area 400 for temporary storage, it is necessary to take out the grinding end and the filter net 201 from the sample bottle 100 to move the sample bottle 100 containing the cell suspension to other equipment for the next processing step to perform subsequent processing of the cell suspension. In order to improve the transfer efficiency and reduce the difficulty of removing the filter net 201, the grinding end of the grinding part provided in this embodiment can move linearly along the first axis a in a direction away from the filter net 201 under the drive of an external force, so that the grinding end moves from the filtering position to the limiting position. When the grinding end is in the limiting position, the grinding end can be in limiting abutment with the filter net 201. At this time, when the grinding end is continuously moved along the first axis a in a direction away from the filter net 201, the grinding end can drive the filter net 201 to move synchronously along the first axis a, so as to realize the synchronous removal of the grinding part and the filter net 201 from the sample bottle 100.

[0039] Specifically, continue to refer to Figure 5 and Figure 6As shown, the grinding end includes a grinding rotor 202, a grinding stator 203, and a fixing member 204. Among them, after the grinding end is placed in the sample bottle 100, the grinding stator 203 is fixedly arranged in the sample bottle 100 and does not rotate around the first axis a. This fixation can be achieved through a positioning structure, such as a positioning groove and a positioning block. One of the positioning groove and the positioning block is arranged on the inner wall surface of the sample bottle 100, and the other is arranged on the grinding stator 203. A rotating shaft hole 2033 is provided through the grinding stator 203 along the direction of the first axis a, and a grinding surface 2031 is provided on the grinding stator 203. The grinding surface 2031 is the bottom surface of the tissue accommodation area 300. The biological tissue entering the tissue accommodation area 300 is located on the grinding surface 2031 and is cut and ground by the grinding rotor 202 on the grinding surface 2031.

[0040] Optionally, the grinding stator 203 is in the shape of a frustum of a cone and includes a conical surface, which is the grinding surface 2031. Setting the grinding surface 2031 as a conical surface can increase the area of the grinding surface 2031, enable more biological tissue to be carried on the grinding surface 2031, and thus improve the grinding efficiency. Further optionally, grinding grooves 2032 are provided on the grinding surface 2031. The number of the grinding grooves 2032 is multiple. The multiple grinding grooves 2032 rotate around the first axis a and are in a spiral shape. The setting of the grinding grooves 2032 can further improve the grinding efficiency and grinding effect. According to requirements, multiple layers of grinding grooves 2032 can be provided on the grinding surface 2031. Each layer of the grinding grooves 2032 includes multiple grinding grooves 2032, and the multiple grinding grooves 2032 in each layer rotate around the first axis a in one direction. The two adjacent layers of grinding grooves 2032 can be arranged in a staggered manner or connected in sequence, and the spiral directions can be the same or different.

[0041] Furthermore, in order to enable the filter net 201 to be stably placed in the sample bottle 100, as Figure 4 shown, a positioning step 103 is provided on the inner wall surface of the sample bottle 100, and the filter net 201 can be placed on the positioning step 103. Since the grinding stator 203 is placed above the filter net 201, in order to enable the grinding stator 203 to be stably in the sealed position, an annular convex edge can be protruded upward along the circumference of the filter net 201, and the bottom of the positioning step 103 is set as a stepped portion. The annular horizontal plane on the stepped portion abuts against the top surface of the annular convex edge, and the portion of the stepped portion below the annular horizontal plane is placed inside the annular convex edge.

[0042] Further, in order to prevent the grinding stator 203 from rotating with the grinding rotor 202 during the grinding process, which may lead to a deteriorated grinding effect, a third limiting structure is provided between the grinding stator 203 and the sample bottle 100 to limit the rotation of the grinding stator 203 about the first axis a. Specifically, the third limiting structure includes a limiting block 104 and a limiting groove. The limiting block 104 is disposed on the inner wall surface of the sample bottle 100, more specifically, it can be disposed on the positioning step 103. The limiting groove is disposed on the outer wall surface of the grinding stator 203, and the limiting block 104 can be inserted into the limiting groove. Of course, in other embodiments, the positions of the limiting block 104 and the limiting groove can be interchanged, that is, the limiting groove is disposed on the inner wall surface of the sample bottle 100, and the limiting block 104 is disposed on the outer wall surface of the grinding stator 203. Of course, in other embodiments, the third limiting structure can also be disposed between the grinding stator 203 and the filter net 201, which will not be elaborated here.

[0043] Continue to refer to Figure 4 and Figure 5 As shown, the grinding rotor 202 includes a rotor shaft 2021 and grinding blades 2022. Among them, the rotor shaft 2021 is a columnar structure, which includes two ends, a first end and a second end. The first end of the rotor shaft 2021 is used to connect to a driving component. And a bottle cap 205 for sealing the sample bottle 100 can also be disposed on the first end of the rotor shaft 2021. The bottle cap 205 can seal the tissue accommodation area 300 to prevent foreign objects from entering the tissue accommodation area 300 during the tissue dissociation process. The second end of the rotor shaft 2021 movably passes through the rotating shaft hole 2033. Here, the second end can be located inside the rotating shaft hole 2033 or protrude from the rotating shaft hole 2033 and be located below the grinding stator 203. The grinding blade 2022 is a curved blade. One end of the grinding blade 2022 is connected to the middle of the rotor shaft 2021, and the other end extends downward in a spiral shape. When the second end of the rotor shaft 2021 is inserted into the rotating shaft hole 2033, the other end of the grinding blade 2022 contacts the grinding surface 2031 of the grinding stator 203. When the rotor shaft 2021 is driven by the driving component and rotates about the first axis a, the other end of the grinding blade 2022 can move on the grinding surface 2031 of the grinding stator 203, and cut and grind the biological tissue located on the grinding surface 2031, so that the shredded biological tissue is mixed evenly with the dissociation solution and is dissociated by the dissociation solution.

[0044] Optionally, the rotor shaft 2021 can be a cylindrical shaft or a stepped shaft. In this embodiment, a stepped shaft is selected to facilitate the positioning and installation of the grinding blade 2022. Specifically, the rotor shaft 2021 includes a first shaft with a larger diameter and a second shaft with a smaller diameter. One end of the grinding blade 2022 is connected to the connection part of the first shaft and the second shaft, and the other end spirally extends to a position flush with the second end of the rotor shaft 2021. To improve the grinding efficiency, two or more grinding blades 2022 can be provided. The multiple grinding blades 2022 are evenly distributed around the circumference of the rotor shaft 2021 and have the same helix direction. To increase the contact area between the grinding blade 2022 and the grinding surface 2031, a grinding edge is inclinedly provided at the other end of the grinding blade 2022. The grinding edge is arranged along the curvature of the grinding surface 2031, and the grinding edge can move on the conical surface to cut biological tissues.

[0045] Further optionally, the end of the rotor shaft 2021 far from the grinding stator 203 is the driving end of the grinding part, and a connection head for connecting the driving component is provided thereon. The bottle cap 205 is detachably sleeved outside the connection head. Through this connection head, the driving component can be detachably connected to the rotor shaft 2021, so as to drive the grinding rotor 202 to rotate relative to the grinding stator 203, thereby realizing the grinding of biological tissues.

[0046] Continue to refer to Figure 4 and Figure 5 As shown, the fixing member 204 is used to relatively fix the grinding rotor 202 and the grinding stator 203, so as to make it possible for the grinding end of the grinding stator 203 to move from the sealing position to the filtering position, and is used to relatively limit the grinding member and the filter net 201, so as to make it possible for the grinding end of the grinding stator 203 to move from the filtering position to the limiting position and realize the synchronous movement of the grinding stator 203, the grinding rotor 202 and the filter net 201.

[0047] Specifically, a through hole is provided in the middle of the filter net 201. The fixing member 204 includes two ends, namely a connection end and a limiting end. The size of the connection end of the fixing member 204 is smaller than the size of the through hole, and it can pass through the through hole and be connected to the second end of the rotor shaft 2021. And a first limiting structure capable of passing through the through hole and limiting the grinding stator 203 is provided in the middle of the fixing member 204, and a second limiting structure is provided at the limiting end of the fixing member 204. The second limiting structure can be limited on the side of the filter net 201 far from the grinding stator 203.

[0048] More specifically, the fixing member 204 is a stepped shaft, and the outer diameter at any position of the stepped shaft is smaller than the size of the through hole, so that the stepped shaft can pass through the through hole. More specifically, continue to refer to Figure 6As shown, the stepped shaft includes a small-diameter shaft 2042 and a large-diameter shaft 2043 that are coaxially connected. One end of the small-diameter shaft 2042 away from the large-diameter shaft 2043 is the connection end, which is connected to the second end of the rotor shaft 2021. The first limiting structure is a first limiting surface 2041 formed between the small-diameter shaft 2042 and the large-diameter shaft 2043. The rotating shaft hole 2033 is a stepped hole, which includes a small-diameter hole and a large-diameter hole connected in sequence. The large-diameter hole is close to the filter screen 201. A second limiting surface 2034 is formed between the small-diameter hole and the large-diameter hole. The first limiting surface 2041 can be in limiting contact with the second limiting surface 2034. The second limiting structure is a limiting boss 2044. The limiting boss 2044 is arranged at one end of the large-diameter shaft 2043 away from the small-diameter shaft 2042, and the size of the limiting boss 2044 is larger than the size of the through hole.

[0049] That is to say, the diameter of the small-diameter shaft 2042 is smaller than the diameter of the small-diameter hole, the diameter of the large-diameter shaft 2043 is smaller than the diameter of the large-diameter hole, and larger than the size of the small-diameter hole. When the grinding stator 203 is in the sealed position, the first limiting surface 2041 and the second limiting surface 2034 are separated. After grinding is completed, when the rotor shaft 2021 of the grinding rotor 202 moves upward under the action of an external force, the small-diameter shaft 2042 gradually penetrates into the small-diameter hole, the large-diameter shaft 2043 gradually penetrates into the large-diameter hole, and the first limiting surface 2041 and the second limiting surface 2034 gradually approach. After the first limiting surface 2041 contacts the second limiting surface 2034, the relative position between the fixing member 204 and the grinding stator 203 remains fixed. At this time, if the rotor shaft 2021 of the grinding rotor 202 continues to move upward under the action of an external force, then the grinding stator 203 can move upward synchronously with the grinding rotor 202. During the synchronous upward movement of the grinding stator 203, the grinding rotor 202 and the fixing member 204, the limiting boss 2044 located below the filter screen 201 gradually approaches the filter screen 201. Since the size of the limiting boss 2044 is larger than the size of the through hole on the filter screen 201, the limiting boss 2044 cannot pass through the through hole. After the limiting boss 2044 abuts against the filter screen 201, if the grinding rotor 202 continues to move upward under the action of an external force, then the filter screen 201 can move upward synchronously with the grinding rotor 202, the grinding stator 203 and the fixing member 204 until the filter screen 201, the grinding rotor 202, the grinding stator 203 and the fixing member 204 are removed from the sample bottle 100.

[0050] Optionally, the limiting boss 2044 may be an annular boss circumferentially arranged around the large-diameter shaft 2043, or may be a plurality of arc-shaped blocks, and the plurality of arc-shaped blocks are circumferentially distributed around the large-diameter shaft 2043. Of course, in addition to being a stepped shaft, the fixing member 204 may also include a cylindrical shaft and an annular boss provided in the middle of the cylindrical shaft. The diameter of the annular boss is smaller than the diameter of the through hole, smaller than the size of the large-diameter hole, and larger than the size of the small-diameter hole.

[0051] Compared with the existing tissue dissociation device, the tissue dissociation device provided in this embodiment has the following advantages:

[0052] 1. It can achieve grinding in the dissociation solution, and the grinding is more sufficient.

[0053] 2. It can be stirred during the incubation process and achieve sufficient contact between the biological tissue and the dissociation solution.

[0054] 3. After sufficient grinding, it can be directly filtered, and the negative pressure method is used for filtration, and the filtration effect is better, which is conducive to obtaining a high-quality and highly active cell suspension.

[0055] 4. The sample bottle 100 can be directly placed on the centrifuge, reducing the operational inconvenience and various existing risks brought by the sample transfer process.

[0056] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, rather than limiting the implementation manners of the present invention. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A tissue dissociation device, characterized in that, Comprising: A sample bottle (100) having a storage space (101) and a storage opening (102); A grinding and filtering structure (200) including a grinding member and a filter net (201), the filter net (201) being movably connected to the grinding end of the grinding member, the grinding end and the filter net (201) being capable of entering the storage space (101) through the storage opening (102), and dividing the storage space (101) into a cell suspension containing area (400) on one side of the filter net (201) and a tissue containing area (300) on one side of the grinding end, and the grinding end having a sealing position in which it abuts against the filter net (201) to seal the cell suspension containing area (400) and a filtering position in which it is separated from the filter net (201); The grinding end is configured to be able to rotate around a first axis a within the sample bottle (100) when in the sealing position to grind biological tissue located in the tissue containing area (300); and the grinding end is configured to be able to move linearly along the first axis a in a direction away from the filter net (201) and move from the sealing position to the filtering position, so that the cell suspension formed by grinding the biological tissue can be filtered through the filter net (201) under negative pressure and enter the cell suspension containing area (400); The grinding end includes a grinding rotor (202), a grinding stator (203) and a fixing member (204), the grinding rotor (202) includes a rotor shaft (2021) and grinding blades (2022), a through hole is provided in the middle of the filter net (201), the connecting end of the fixing member (204) can pass through the through hole and be connected to the second end of the rotor shaft (2021), and a first limiting structure capable of passing through the through hole and limiting with the grinding stator (203) is provided on the fixing member (204), and a second limiting structure is provided at the limiting end of the fixing member (204), and the second limiting structure can be limited on the side of the filter net (201) away from the grinding stator (203).

2. The tissue dissociation device according to claim 1, wherein The grinding end is configured to be able to continue to move linearly along the first axis a in a direction away from the filter net (201) and move from the filtering position to a limiting position, and the grinding end in the limiting position can be in limiting abutment with the filter net (201), so that the grinding end can drive the filter net (201) to move synchronously along the first axis a.

3. The tissue dissociation device according to claim 2, wherein A rotating shaft hole (2033) is provided through the grinding stator (203) along the first axis a direction, and a grinding surface (2031) is provided on the grinding stator (203); One end of the grinding blade (2022) is connected to the middle of the rotor shaft (2021). The first end of the rotor shaft (2021) is used to connect to a driving component. The second end of the rotor shaft (2021) movably passes through the rotating shaft hole (2033) so that the other end of the grinding blade (2022) contacts the grinding surface (2031) of the grinding stator (203). When the grinding rotor (202) is configured to rotate, the grinding blade (2022) can grind the biological tissue located on the grinding surface (2031).

4. The tissue dissociation device according to claim 3, wherein The fixing member (204) is a stepped shaft. The outer diameter of the stepped shaft is smaller than the size of the through hole. The stepped shaft includes a small-diameter shaft (2042) and a large-diameter shaft (2043) connected coaxially. One end of the small-diameter shaft (2042) away from the large-diameter shaft (2043) is connected to the second end of the rotor shaft (2021). The first limiting structure is a first limiting surface (2041) formed between the small-diameter shaft (2042) and the large-diameter shaft (2043); The rotating shaft hole (2033) is a stepped hole, which includes a small-aperture hole and a large-aperture hole connected in sequence. The large-aperture hole is arranged close to the filter net (201). A second limiting surface (2034) is formed between the small-aperture hole and the large-aperture hole. The first limiting surface (2041) can be in limiting abutment with the second limiting surface (2034).

5. The tissue dissociation device according to claim 4, wherein The second limiting structure is a limiting boss (2044). The limiting boss (2044) is arranged at one end of the large-diameter shaft (2043) away from the small-diameter shaft (2042), and the size of the limiting boss (2044) is larger than the size of the through hole.

6. The tissue dissociation device according to claim 3, wherein The grinding surface (2031) is a conical surface. The other end of the grinding blade (2022) is inclined with a grinding edge. The grinding edge can move on the conical surface to cut the biological tissue.

7. The tissue dissociation device according to claim 3, wherein A plurality of grinding grooves (2032) are arranged on the grinding surface (2031). The plurality of grinding grooves (2032) rotate around the first axis a.

8. The tissue dissociation device according to claim 1, wherein A positioning step (103) is arranged on the inner wall surface of the sample bottle (100). The filter net (201) can be placed on the positioning step (103).

9. The tissue dissociation device according to claim 3, wherein A third limiting structure is arranged between the grinding stator (203) and the sample bottle (100) to limit the grinding stator (203) from rotating around the first axis a.

10. The tissue dissociation device according to claim 9, wherein The third limiting structure includes a limiting block (104) arranged on the inner wall surface of the sample bottle (100) and a limiting groove arranged on the outer wall surface of the grinding stator (203), and the limiting block (104) can be inserted into the limiting groove.

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