A device and method for detecting biochemical substances based on optical fiber composite liquid crystal

Abstract

The invention discloses a device and method for detecting biochemical substances based on optical fiber composite liquid crystal, the device includes a reaction pool, a detection reagent input end, a waste liquid collection end, an optical signal transmitting end and an optical signal receiving and processing end; a glass chip is placed on the inner bottom surface of the reaction pool, and liquid crystal is placed on the surface of the glass chip; the top surface of the reaction pool has an optical signal input port and an optical signal output port; the detection reagent input end is connected to the reaction pool and is used to inject a reagent that reacts with the liquid crystal into the reaction pool; the waste liquid collection end is connected to the reaction pool and is used to discharge the reagent waste liquid after the reaction; the optical signal transmitting end is connected to the optical signal input port; the optical signal receiving and processing end is connected to the optical signal output port. The test reagent reacts with the liquid crystal, a light source is introduced through the optical fiber to irradiate the reaction liquid, and the optical signal reflected after the irradiation is received by a spectrometer, and then the signal output by the spectrometer is calculated by algorithm, thereby realizing the detection of the reagent.

Description

Technical Field

[0001] The present invention relates to the field of optical fiber sensing technology, and more specifically to a device and method for detecting biochemical substances based on optical fiber composite liquid crystal. Background Art

[0002] Liquid crystal is a substance between liquid and solid. It can flow like a liquid, but can also keep its molecules in an orderly arrangement like a solid crystal. It has some properties of both liquid and crystal. Changes in the external environment can change the arrangement of its molecules, causing it to present different optical properties.

[0003] Since Abbott et al. built the liquid crystal biosensor platform in the 1990s, liquid crystals have shown great application value or potential in display devices, photoelectric modulation devices, temperature sensors, and biosensors, attracting the attention of scientific researchers.

[0004] So far, domestic and foreign researchers have made a lot of explorations in the field of liquid crystal sensing. For example, "Modern Scientific Instruments" published "Research Progress on Chemical Sensing Mechanism and Application Based on Liquid Crystal Orientation Change" co-authored by Li Ming et al. in 2006 (06), "Direct Electrochemistry and Biosensing Applications of Liquid Crystal Cubic Phase Encapsulated Hemoglobin" published by Yao Zhen et al. at the 11th National Electroanalytical Chemistry Conference in 2011, "Temperature Sensing Characteristics of Total Internal Reflection Photonic Crystal Fiber Based on Liquid Crystal Filling" by Qian Xiangzhong in 2009, 26 (03), "Analytical Chemistry" published "New Liquid Crystal Biosensor Based on Nucleic Acid Aptamer for Detection of Platelet-Derived Growth Factor BB" co-authored by Li Xia et al. in 2014, 42 (05), and "Research Progress on Chemical and Biosensing Technology Based on Liquid Crystal Orientation Change" co-authored by Zuo Fang et al. in 2011, 39 (12).

[0005] However, liquid crystal is still in the exploratory stage in biochemical analysis. Most of the above methods use the primary method of naked eye observation under a microscope to detect biochemical substances, and there are still problems such as immature detection technology and detection equipment.

[0006] Therefore, how to provide a device and method for detecting biochemical substances based on optical fiber composite liquid crystal is a problem that technical personnel in this field need to solve urgently. Summary of the invention

[0007] In view of this, the present invention provides a device and method for detecting biochemical substances based on optical fiber composite liquid crystal, aiming to solve the above technical problems.

[0008] In order to achieve the above object, the present invention adopts the following technical solution:

[0009] A device for detecting biochemical substances based on optical fiber composite liquid crystal, comprising:

[0010] A reaction pool; a glass chip is placed on the inner bottom surface of the reaction pool, and liquid crystal is placed on the surface of the glass chip; a light signal input port and a light signal output port are provided on the top surface of the reaction pool, and the light signal input port and the light signal output port form an entry point and a reflection receiving point of light with the liquid crystal as a reflection point;

[0011] A detection reagent input end; the detection reagent input end is connected to the reaction pool and is used to inject a reagent that reacts with the liquid crystal into the reaction pool;

[0012] A waste liquid collection end; the waste liquid collection end is connected to the reaction tank and is used to discharge the reagent waste liquid after the reaction;

[0013] An optical signal transmitting end; the optical signal transmitting end is connected to the optical signal input port;

[0014] Optical signal receiving and processing end; the optical signal receiving and processing end is connected to the optical signal output port, and performs algorithm processing on the received optical signal, thereby realizing the detection of the reagent.

[0015] Through the above technical scheme, the present invention realizes the injection and discharge of biochemical substances through the detection reagent input end and the waste liquid collection end, satisfies the reaction between the biochemical substances and the liquid crystal, inputs and collects the optical signal through the cooperation of the optical signal transmitting end and the optical signal receiving and processing end, and then performs detection, which is not subject to the human error caused by naked eye observation, making the detection result more reliable.

[0016] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the detection reagent input end includes a light source, and a first optical fiber bundle connected to the emitting end of the light source; the end of the first optical fiber bundle away from the light source is connected to the optical signal input port, which can meet the optical signal output of the light source.

[0017] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the light source is a polarized light source, which effectively ensures the recognition performance of the liquid crystal signal during the measurement process.

[0018] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the optical signal receiving and processing end includes a second optical fiber bundle connected to the optical signal output port, and a spectrometer connected to the end of the second optical fiber bundle away from the detection signal output port, and a signal acquisition module, an A / D conversion module, a single-chip microcomputer and a display screen electrically connected to the output port of the spectrometer in sequence. The single-chip microcomputer performs algorithm processing on the feedback signal and transmits it to the display screen to display the detection result.

[0019] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the detection reagent input end includes a reagent tube and a sample injection turntable connected to the reagent tube; the sample injection turntable is connected to a peristaltic pump through a latex tube, and the peristaltic pump is connected to the reaction pool through a liquid inlet tube. The sample injection turntable can hold multiple groups of reagents, which can be introduced into the sample injection turntable through a hose or directly poured into the sample injection turntable, and then injected into the reaction pool through the cooperation of the latex tube and the peristaltic pump to react with the liquid crystal.

[0020] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the waste liquid collection end includes a drainage pipe connected to the reaction pool and a waste liquid collection pipe connected to the drainage pipe, so as to collect the waste liquid after the reaction.

[0021] Preferably, in the above-mentioned device for detecting biochemical substances based on optical fiber composite liquid crystal, the lower surface of the glass chip is coated with a reflective film, which can improve the reflection effect.

[0022] The present invention also provides a method for detecting biochemical substances based on optical fiber composite liquid crystal, in which a test agent is reacted with liquid crystal, a light source is introduced through the optical fiber to irradiate the reaction liquid, and the light signal reflected after irradiation is received by a spectrometer, and then an algorithm is calculated for the signal output by the spectrometer, thereby realizing the detection of the agent.

[0023] Through the above technical scheme, the present invention uses optical signals to detect reagents that react with liquid crystals, and has the advantages of real-time, label-free, low cost, and simple operation when detecting biochemical substances.

[0024] Preferably, in the above-mentioned method for detecting biochemical substances based on optical fiber composite liquid crystal, the algorithm adopts a frame brightness algorithm or a variable pixel RGB color value algorithm. The frame brightness algorithm is adopted to track the change of the average light intensity of each frame image to obtain the detection result; or the variable pixel RGB color value algorithm is adopted to detect the result by comparing the change of the RGB color value of the pixel from one frame to the next frame.

[0025] Preferably, in the above-mentioned method for detecting biochemical substances based on optical fiber composite liquid crystal, the liquid crystal surface is modified with a biochemical molecule recognition element corresponding to the biochemical molecule to be detected. The modification of the liquid crystal surface further includes the modification of various nanomaterials. The present invention can detect many substances, and different substances can be detected by modifying different functional films, such as glucose, algae toxins, hormones, mycotoxin, etc.

[0026] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a device and method for detecting biochemical substances based on optical fiber composite liquid crystal, which has the following beneficial effects:

[0027] 1. The present invention is not subject to human error caused by naked eye observation, making the detection result more reliable.

[0028] 2. Since the present invention directly adopts a polarized light source, the recognition performance of the liquid crystal signal during the measurement process is effectively guaranteed.

[0029] 3. The present invention has the advantages of real-time monitoring, label-free, low cost, and easy operation when detecting biochemical substances. It is expected to play an important role in the fields of biological analysis, disease diagnosis, and environmental monitoring, and has broad commercial application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0031] Figure 1 The accompanying drawing is a schematic diagram of the structure of the device for detecting biochemical substances based on optical fiber composite liquid crystal provided by the present invention.

[0032] in:

[0033] 1 is the light source, 2 is the first optical fiber bundle, 3 is the reagent tube, 4 is the sample injection turntable, 5 is the latex tube, 6 is the peristaltic pump, 7 is the liquid inlet tube, 8 is the reaction pool, 9 is the liquid crystal, 10 is the glass chip, 11 is the liquid discharge tube, 12 is the waste liquid collection tube, 13 is the second optical fiber bundle, 14 is the spectrometer, 15 is the signal acquisition module, 16 is the A / D conversion module, 17 is the single-chip microcomputer, 18 is the display screen; 19 is the optical signal input port; 20 is the optical signal output port. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] See attached Figure 1 The embodiment of the present invention discloses a device for detecting biochemical substances based on optical fiber composite liquid crystal, comprising:

[0036] A reaction pool 8; a glass chip 10 is placed on the inner bottom surface of the reaction pool 8, and a liquid crystal 9 is placed on the surface of the glass chip 10; the top surface of the reaction pool 8 has an optical signal input port 19 and an optical signal output port 20, and the optical signal input port 19 and the optical signal output port 20 form an entry point and a reflection receiving point of light with the liquid crystal 9 as a reflection point;

[0037] Detection reagent input terminal; the detection reagent input terminal is connected to the reaction pool 8 and is used to inject a reagent that reacts with the liquid crystal 9 into the reaction pool 8;

[0038] Waste liquid collection end; the waste liquid collection end is connected to the reaction tank 8 and is used to discharge the reagent waste liquid after the reaction;

[0039] The optical signal transmitting end is connected to the optical signal input port 19;

[0040] Optical signal receiving and processing end; the optical signal receiving and processing end is connected to the optical signal output port 20, and performs algorithm processing on the received optical signal, thereby realizing the detection of the reagent.

[0041] In order to further optimize the above technical solution, the detection reagent input end includes a light source 1 and a first optical fiber bundle 2 connected to the emission end of the light source 1; the end of the first optical fiber bundle 2 away from the light source 1 is connected to the optical signal input port 19.

[0042] In order to further optimize the above technical solution, the light source 1 is a polarized light source.

[0043] In order to further optimize the above technical solution, the optical signal receiving and processing end includes a second optical fiber bundle 13 connected to the optical signal output port 20, a spectrometer 14 connected to the end of the second optical fiber bundle 13 away from the detection signal output port 20, and a signal acquisition module 15, an A / D conversion module 16, a single-chip microcomputer 17 and a display screen 18 electrically connected to the output port of the spectrometer 14 in sequence.

[0044] In order to further optimize the above technical solution, the detection reagent input end includes a reagent tube 3 and an injection turntable 4 connected to the reagent tube 3; the injection turntable 4 is connected to a peristaltic pump 6 through a latex tube 5, and the peristaltic pump 6 is connected to a reaction pool 8 through a liquid inlet tube 7.

[0045] In order to further optimize the above technical solution, the waste liquid collection end includes a drain pipe 11 connected to the reaction tank 8 and a waste liquid collection pipe 12 connected to the drain pipe 11 .

[0046] In order to further optimize the above technical solution, the lower surface of the glass chip 10 is coated with a reflective film.

[0047] The method of the device for detecting biochemical substances based on optical fiber composite liquid crystal provided in this embodiment includes the following steps:

[0048] ① The reagent in the reagent tube 3 enters the latex tube 5 through the sample injection turntable 4;

[0049] ② Then enter the liquid inlet pipe 7 through the peristaltic pump 6;

[0050] ③ Then it flows into the reaction tank 8 through the liquid inlet pipe 7 to react;

[0051] ④ The liquid after the reaction is discharged to the waste liquid collection pipe 12 through the drain pipe 11;

[0052] ⑤ The light emitted by the light source 1 is irradiated onto the liquid crystal 9 via the first optical fiber bundle 2;

[0053] ⑥ The light reflected by the liquid crystal 9 enters the second optical fiber bundle 13;

[0054] ⑦ The second optical fiber bundle 13 transmits the reflected signal to the spectrometer 14;

[0055] ⑧ The spectrometer 14 further transmits the signal to the signal acquisition module 15;

[0056] ⑨A / D conversion module 16 converts the collected signal into digital information;

[0057] ⑩ The single chip microcomputer 17 performs algorithm analysis and processing on the converted data;

[0058] The data processed by the algorithm analysis is displayed on the display screen 18 in the form of a chart.

[0059] The second optical fiber bundle 13 coincides with the reflected light of the light beam emitted by the first optical fiber bundle 2 on the reflected light of the light beam of the first optical fiber bundle 2 to ensure that the light emitted by the first optical fiber bundle 2 enters the second optical fiber bundle 13 after being reflected on the surface of the liquid crystal 9.

[0060] The liquid crystal 9 is located in the middle of the upper surface of the glass chip 10 and is within the light spot range of the first optical fiber bundle 2. The lower surface of the glass chip 10 is coated with a reflective film. The surface of the liquid crystal 9 is modified with a specific biochemical molecule recognition element to achieve the detection of specific biochemical molecules. The modification of the surface of the liquid crystal 9 further includes the modification of various nanomaterials.

[0061] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0062] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for detecting biochemical substances based on optical fiber composite liquid crystal, characterized in that: include: A reaction pool (8); a glass chip (10) is disposed on the inner bottom surface of the reaction pool (8), and a liquid crystal (9) is placed on the surface of the glass chip (10); a light signal input port (19) and a light signal output port (20) are provided on the top surface of the reaction pool (8), and the light signal input port (19) and the light signal output port (20) form a light entry point and a reflection receiving point with the liquid crystal (9) as a reflection point; a detection reagent input end; the detection reagent input end is connected to the reaction pool (8) and is used to inject a reagent that reacts with the liquid crystal (9) into the reaction pool (8); A waste liquid collection end; the waste liquid collection end is connected to the reaction tank (8) and is used to discharge the reagent waste liquid after the reaction; An optical signal transmitting end; the optical signal transmitting end is connected to the optical signal input port (19); An optical signal receiving and processing end; the optical signal receiving and processing end is connected to the optical signal output port (20) and performs algorithm processing on the received optical signal, thereby realizing detection of the reagent; The reagent to be tested reacts with the liquid crystal, and a light source is introduced through an optical fiber to irradiate the reaction liquid. The reflected light signal after irradiation is received by a spectrometer, and then an algorithm is calculated for the signal output by the spectrometer to achieve the detection of the reagent; The liquid crystal surface is modified with biochemical molecule recognition elements corresponding to the biochemical molecules to be detected.

2. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 1, characterized in that: The detection reagent input end comprises a light source (1) and a first optical fiber bundle (2) connected to the emission end of the light source (1); an end of the first optical fiber bundle (2) away from the light source (1) is connected to the optical signal input port (19).

3. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 2, characterized in that: The light source (1) is a polarized light source.

4. A method for detecting biochemical substances based on optical fiber composite liquid crystal according to any one of claims 1 to 3, characterized in that: The optical signal receiving and processing end comprises a second optical fiber bundle (13) connected to the optical signal output port (20), a spectrometer (14) connected to an end of the second optical fiber bundle (13) away from the optical signal output port (20), and a signal acquisition module (15), an A / D conversion module (16), a single-chip microcomputer (17) and a display screen (18) electrically connected in sequence to an output port of the spectrometer (14).

5. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 1, characterized in that: The detection reagent input end comprises a reagent tube (3) and a sample injection turntable (4) connected to the reagent tube (3); the sample injection turntable (4) is connected to a peristaltic pump (6) via a latex tube (5), and the peristaltic pump (6) is connected to the reaction pool (8) via a liquid inlet tube (7).

6. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 1, characterized in that: The waste liquid collection end comprises a liquid discharge pipe (11) connected to the reaction tank (8), and a waste liquid collection pipe (12) connected to the liquid discharge pipe (11).

7. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 1, characterized in that: The lower surface of the glass chip (10) is coated with a reflective film.

8. The method for detecting biochemical substances based on optical fiber composite liquid crystal according to claim 1, characterized in that: The algorithm adopts a frame brightness algorithm or a variable pixel RGB color value algorithm.

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