Automated microliter liquid quantitative dispensing device and method

Abstract

The present invention provides an automated microliter liquid quantitative distribution device and method, comprising: a fluid channel module, a driving module and a fluid export module; the fluid channel module is provided with a first inflow channel, a quantitative chamber and an outflow channel, and the first inflow channel, the quantitative chamber and the outflow channel are connected; the first inflow channel is provided with a first fluid introduction valve, and the outflow channel is provided with a first fluid push-out valve; a quantitative push rod is movably provided in the quantitative chamber; the driving module is used to control the working state of the first fluid introduction valve, the first fluid push-out valve and the quantitative push rod; the fluid export module is provided at one end of the outflow channel away from the fluid push-out valve to receive the sample flowing out of the outflow channel. The present invention can realize the automated quantitative distribution of sample microliter liquid, thereby improving the distribution efficiency of the sample.

Description

Technical Field

[0001] The present invention relates to the technical field of automated quantitative liquid addition, and in particular to an automated microliter liquid quantitative dispensing device and method. Background Art

[0002] At present, most of the pretreatment and analysis devices for various samples rely on manual operation and rely on precise liquid pipetting devices and equipment to sample liquids. They have high requirements on manpower and require professional training to use related equipment and instruments. The existing quantitative distribution of samples includes multiple complex steps. Whether it is completed manually or with the help of auxiliary equipment, it has shortcomings such as low efficiency and easy errors. In particular, the stable separation and transfer of small volumes of liquids has always been a requirement of various analytical methods, but mainstream automated analytical instruments cannot solve the technical difficulties well, let alone achieve the miniaturization of equipment.

[0003] Therefore, how to achieve the automated distribution of samples in microliter quantities and improve the efficiency of quantitative distribution of samples and the accuracy of microquantification is an urgent problem to be solved in this project. Summary of the invention

[0004] The present invention provides an automated microliter liquid quantitative distribution device and method, which are used to solve the problems of low sample distribution efficiency and difficulty in automatic quantitative distribution of trace liquids in the prior art, and realize automated microliter liquid quantitative distribution of samples.

[0005] The present invention provides an automated microliter liquid quantitative dispensing device, comprising: a fluid channel module, a driving module and a fluid export module;

[0006] The fluid channel module is provided with a first inflow channel, a quantitative chamber and an outflow channel, and the first inflow channel, the quantitative chamber and the outflow channel are connected;

[0007] The first inflow channel is provided with a first fluid introduction valve, and the outflow channel is provided with a first fluid push-out valve; a quantitative push rod is movably provided in the quantitative chamber; and the driving module is used to control the working states of the first fluid introduction valve, the first fluid push-out valve and the quantitative push rod;

[0008] The fluid outlet module is disposed at one end of the outflow channel away from the fluid push-out valve to receive the sample flowing out of the outflow channel.

[0009] According to an automated microliter liquid quantitative dispensing device provided by the present invention, the driving module comprises a first driving member, a second driving member and a third driving member;

[0010] The first driving member is connected to the quantitative push rod to control the position of the quantitative push rod; the second driving member and the third driving member are respectively connected to the first fluid inlet valve and the first fluid outlet valve to control the opening and closing of the first fluid inlet valve and the first fluid outlet valve.

[0011] According to an automated microliter liquid quantitative dispensing device provided by the present invention, the fluid channel module is provided with a second inflow channel, and the second inflow channel is connected to the outflow channel;

[0012] A second fluid introduction valve is provided between the second inflow channel and the outflow channel, and a second fluid push-out valve is provided in the outflow channel to control the connection and isolation state between the second inflow channel and the outflow channel;

[0013] The second fluid introduction valve and the second fluid ejection valve are controlled by the second driving member and the third driving member respectively.

[0014] According to an automated microliter liquid quantitative dispensing device provided by the present invention, the quantitative chamber and the outflow channel are both provided in plurality, and each of the quantitative chamber and the outflow channel is connected to the inflow channel;

[0015] A fluid introduction valve is provided between the inflow channel and each of the quantitative chambers, and a fluid push-out valve is provided between each of the quantitative chambers and the outflow channel; each of the fluid introduction valves is controlled by the second driving member, and each of the fluid push-out valves is controlled by the third driving member;

[0016] A quantitative push rod is movably arranged in each quantitative chamber.

[0017] According to an automated microliter liquid quantitative dispensing device provided by the present invention, a first limit plate and a second limit plate are respectively provided at both ends of the quantitative push rod, the first limit plate extends into the quantitative chamber, and the second limit plate is located outside the quantitative chamber and connected to the first driving member;

[0018] The inner wall of the quantitative chamber is provided with a push-out positioning block and a push-in positioning block, and the quantitative push rod moves between the push-out positioning block and the push-in positioning block;

[0019] A position feedback sensor is provided at one end of the quantitative push rod extending into the quantitative chamber, and the position feedback sensor is used to feedback the pushing stroke of the quantitative push rod by the first driving member.

[0020] According to an automated microliter liquid quantitative dispensing device provided by the present invention, the position feedback sensor comprises: an electrode arranged on the side of the quantitative push rod;

[0021] A resistor is disposed on the side wall of the quantitative chamber, and the resistor is connected to the electrode to determine the position of the quantitative push rod pushed by the first driving member.

[0022] According to an automated microliter liquid quantitative dispensing device provided by the present invention, a sealing member is provided at one end of the quantitative push rod close to the first limiting plate, the sealing member is provided on the periphery of the quantitative push rod, one end of the sealing member is connected to the second limiting plate, the other end of the sealing member is connected to the third limiting plate, and the third limiting plate is fixedly connected to the rod body of the quantitative push rod.

[0023] According to an automated microliter liquid quantitative dispensing device provided by the present invention, the fluid channel module is connected to a fluid outlet, and the fluid outlet is arranged at the sample outflow end of the outflow channel;

[0024] The other end of the fluid outlet is connected to the fluid outlet module, and one end of the fluid outlet connected to the fluid outlet module is in a constricted shape.

[0025] According to an automated microliter liquid quantitative dispensing device provided by the present invention, a quantitative pushing device is arranged in the fluid discharge port, and the quantitative pushing device comprises: a pneumatic microcapsule arranged on the inner wall of the fluid discharge port, and the pneumatic microcapsule facilitates the sample to fall into the fluid derivation module based on the change of internal air pressure;

[0026] The fluid outlet is provided with a fluid sensor, and the fluid sensor is used to detect and judge the sample of the fluid outlet.

[0027] The present invention also provides an automated microliter liquid quantitative dispensing method, comprising:

[0028] Open the first fluid ejection valve, move the quantitative push rod to the push-in positioning block, and discharge the air in the quantitative chamber from the outflow channel;

[0029] After confirming that the air is discharged, closing the first fluid ejection valve to separate the quantitative chamber from the outflow channel;

[0030] Opening the first fluid introduction valve, moving the quantitative push rod, and injecting the liquid sample in the first inflow channel into the quantitative chamber;

[0031] After the quantitative chamber obtains a quantitative liquid sample from the first inflow channel, the first fluid introduction valve is closed;

[0032] Opening the first fluid ejection valve, moving the quantitative push rod, allowing the liquid sample to be injected from the quantitative chamber into the outflow channel, and discharged from the outflow channel, so that the fluid outlet module can detect the discharged liquid;

[0033] It is determined whether the liquid sample has completely flowed out of the outflow channel. If it is confirmed that the liquid sample has not completely flowed out, the quantitative push rod is moved again to discharge the liquid sample from the outflow channel.

[0034] The automated microliter liquid quantitative dispensing device and method provided by the present invention provide a first inflow channel, a quantitative chamber, and an outflow channel in a fluid channel module, and respectively set a first fluid introduction valve and a first fluid push-out valve in the first inflow channel and the outflow channel, and set a quantitative push rod in the quantitative chamber, and automatically control the working states of the first fluid introduction valve, the first fluid push-out valve, and the quantitative push rod through a driving module to achieve automated quantitative dispensing of sample liquid, thereby improving the sample dispensing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is one of the structural schematic diagrams of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0036] Figure 2 This is the second structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0037] Figure 3 This is the third structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0038] Figure 4 This is the fourth structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0039] Figure 5 This is the fifth structural diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0040] Figure 6 This is the sixth structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0041] Figure 7 This is the seventh structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0042] Figure 8 This is the eighth structural schematic diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0043] Fig. 9 This is the ninth structural diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0044] Fig.10 This is the tenth structural diagram of the first embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0045] Fig.11 It is a structural schematic diagram of Embodiment 2 of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0046] Fig.12 2 is a schematic diagram of the structure of the third embodiment of the automated microliter liquid quantitative dispensing device provided by the present invention;

[0047] Fig.13 2 is a schematic diagram of the structure of a fourth embodiment of an automated microliter liquid quantitative dispensing device provided by the present invention;

[0048] Fig.14 The figure is a flow chart of an embodiment of the automated microliter liquid quantitative dispensing method provided by the present invention.

[0049] : 100, fluid channel module; 200, driving module; 300, fluid export module; 110, first inflow channel; 120, first fluid introduction valve; 130, quantitative push rod; 140, quantitative chamber; 150, first fluid push valve; 160, outflow channel, 170, fluid discharge port; 141, push-out positioning block; 142, push-in positioning block; 132, sealing member; 133, third limit plate; 134, position feedback sensor; 135, light source; 111, first inflow channel; 112, second inflow channel; 122, second fluid introduction valve; 152, second fluid push valve; 171, quantitative pushing device; 172, pneumatic microcapsule; 173, fluid sensor. DETAILED DESCRIPTION

[0050] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] Combine the following Figure 1-Figure 12 The present invention describes an automated microliter liquid quantitative dispensing device.

[0052] Reference Figure 1 The present invention provides an automated microliter liquid quantitative dispensing device, comprising: a fluid channel module 100, a driving module 200 and a fluid export module 300;

[0053] Reference Figure 2The fluid channel module 100 is provided with a first inflow channel 110, a quantitative chamber 140 and an outflow channel 160, and the first inflow channel 110, the quantitative chamber 140 and the outflow channel 160 are connected;

[0054] The first inflow channel 110 is provided with a first fluid introduction valve 120, and the outflow channel 160 is provided with a first fluid push-out valve 150; a quantitative push rod 130 is movably provided in the quantitative chamber 140; the driving module 200 is used to control the working states of the first fluid introduction valve 120, the first fluid push-out valve 150 and the quantitative push rod 130;

[0055] The fluid outlet module 300 is disposed at one end of the outflow channel 160 away from the fluid push-out valve to receive and detect the sample flowing out of the outflow channel 160 .

[0056] Specifically, the fluid channel module 100 serves as a carrier for holding the fluid, and its optional materials include but are not limited to polycarbonate material (PC), polypropylene (PP), polystyrene (PS), polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene plastic (ABS), polylactic acid (PLA), photosensitive resin, stainless steel, carbon steel, aluminum, copper, etc.

[0057] The driving module 200 is used to control the first fluid introduction valve 120, the first fluid push-out valve 150 and the quantitative push rod 130 to coordinate the entire quantitative distribution process. The fluid outlet module 300 is used to detect the fluid after the quantitative distribution of the fluid.

[0058] In this embodiment, the first fluid introduction valve 120 and the first fluid push-out valve 150 are both configured as a rotary valve core, which has a fluid microstructure and can be used to transfer fluid. The quantitative push rod 130 can be configured as a piston structure to control the volume of the fluid in the quantitative chamber 140. The first fluid introduction valve 120, the first fluid push-out valve 150 and the quantitative push rod 130 are used in conjunction with each other through the drive of the drive module 200, and can control the volume and storage position of the fluid.

[0059] Reference Figure 3 Before the quantitative process, the first fluid ejection valve 150 is opened, the quantitative push rod 130 is moved downward, and the air in the quantitative chamber 140 is discharged through the outflow channel 160 to prevent the sample from mixing with air during the quantitative process and causing errors in the quantitative distribution volume.

[0060] Reference Figure 4 During the quantitative process, the first fluid introduction valve 120 is first opened, the first fluid ejection valve 150 is closed, and the quantitative push rod 130 is moved upward, so that the sample flows from the first inflow channel 110 into the quantitative chamber 140 .

[0061] Reference Figure 5 After the quantitative determination is completed, the first fluid ejection valve 150 is opened, the first fluid introduction valve 120 is closed, and the quantitative push rod 130 is moved downward to transfer the sample from the quantitative chamber 140 to the outflow channel 160, and then the sample is discharged to the fluid export module 300 through the outflow channel 160 for detection.

[0062] The automated microliter liquid quantitative dispensing device provided in the embodiment of the present invention is configured by opening a first inflow channel 110, a quantitative chamber 140 and an outflow channel 160 in a fluid channel module 100, and respectively arranging a first fluid introduction valve 120 and a first fluid push-out valve 150 in the first inflow channel 110 and the outflow channel 160, and arranging a quantitative push rod 130 in the quantitative chamber 140. The working states of the first fluid introduction valve 120, the first fluid push-out valve 150 and the quantitative push rod 130 are automatically controlled by a driving module 200 to realize automated quantitative dispensing of sample liquid, thereby improving the sample dispensing efficiency.

[0063] Optionally, the driving module 200 includes a first driving member, a second driving member and a third driving member;

[0064] The first driving member is connected to the metering push rod 130 to control the position of the metering push rod 130; the second driving member and the third driving member are respectively connected to the first fluid inlet valve 120 and the first fluid outlet valve 150, for controlling the opening and closing of the first fluid inlet valve 120 and the first fluid outlet valve 150.

[0065] Specifically, the first driving member can be configured as a power device such as a pneumatic cylinder or an electric cylinder to control the position of the quantitative push rod 130 so that the quantitative push rod 130 can move up and down, thereby controlling the volume of the fluid in the quantitative chamber 140 .

[0066] The second driving member and the third driving member can be configured as a stepping motor, a servo motor, a brushless DC motor or a screw motor, etc., and the opening and closing of the first fluid introduction valve 120 and the first fluid ejection valve 150 can be controlled by the rotation of the motor.

[0067] Optionally, refer to Figure 6 , a first limiting plate and a second limiting plate are respectively provided at both ends of the quantitative push rod 130, the first limiting plate extends into the quantitative chamber 140, and the second limiting plate is located outside the quantitative chamber 140 and connected to the first driving member;

[0068] The inner wall of the quantitative chamber 140 is provided with a push-out positioning block 141 and a push-in positioning block 142, and the quantitative push rod 130 moves between the push-out positioning block 141 and the push-in positioning block 142;

[0069] Reference Figure 7-Figure 9 A position feedback sensor 134 is provided at one end of the quantitative push rod 130 extending into the quantitative chamber 140 , and the position feedback sensor 134 is used to feedback the pushing stroke of the quantitative push rod 130 by the first driving member.

[0070] In this embodiment, the limit plates at both ends of the metering push rod 130 and the push-in positioning block 142 and the push-out positioning block 141 on the inner wall of the metering chamber 140 cooperate to limit the up and down movement of the metering push rod 130 within a certain range, thereby ensuring the volume of the fluid in the metering chamber 140 and preventing the metering push rod 130 from detaching from the metering chamber 140 and causing the fluid to overflow from the metering chamber 140.

[0071] The position feedback sensor 134 is used to achieve non-contact distance sensing and presence detection with the liquid sample. The position feedback sensor 134 can be a high-precision ultrasonic sensor, a time-of-flight (ToF) laser sensor, an image sensor with an optical system, or an image sensor without an optical system.

[0072] It should be noted that the position feedback sensor 134 adopts an image sensor, and a light source 135 is arranged around the position feedback sensor 134 to illuminate the quantitative chamber 140 .

[0073] Optionally, the position feedback sensor 134 includes: an electrode disposed on a side of the quantitative push rod 130;

[0074] A resistor is disposed on the side wall of the quantitative chamber 140 , and the resistor is connected to the electrode to determine the position of the quantitative push rod 130 pushed by the first driving member.

[0075] In this embodiment, an electrode is arranged on the outer side of the quantitative push rod 130, and a precision resistance device that can be connected to the electrode is arranged on the side wall of the quantitative chamber 140. The precise position of the quantitative push rod 130 pushed by the first driving member can be determined through the connected resistance device, thereby feeding back to the control module of the first driving member and optimizing the movement process of the first driving member.

[0076] Optionally, refer to Figure 7 A sealing member 132 is provided at one end of the quantitative push rod 130 close to the first limiting plate. The sealing member 132 is provided at the periphery of the quantitative push rod 130. One end of the sealing member 132 is connected to the second limiting plate. The other end of the sealing member 132 is connected to the third limiting plate 133. The third limiting plate 133 is fixedly connected to the rod body of the quantitative push rod 130.

[0077] In this embodiment, one end of the quantitative push rod 130 extending into the quantitative chamber 140 is set as a piston structure, that is, a seal 132 is set at one end of the quantitative push rod 130. The seal 132 is made of soft sealing material, including but not limited to silicone, rubber, etc.

[0078] The seal 132 is fixed on the first limit plate, and the fixing method can be adhesive, bolt fixing or other mechanical fixing, which is not limited here. The seal 132 plays a sealing role to prevent the fluid in the quantitative chamber 140 from flowing through the quantitative push rod 130 and overflowing, and the seal 132 can ensure the reliability and stability of the quantitative push rod 130 moving in the quantitative chamber 140.

[0079] Optionally, refer to Figure 2 , the fluid channel module 100 is connected to a fluid outlet 170 , and the fluid outlet 170 is arranged at the sample outflow end of the outflow channel 160 ;

[0080] The other end of the fluid outlet 170 is connected to the fluid outlet module 300 , and one end of the fluid outlet 170 connected to the fluid outlet module 300 is in a constricted shape.

[0081] In this embodiment, the fluid outlet 170 is arranged at the bottom end of the outflow channel 160, that is, the sample outflow end, and the liquid outlet end of the outlet is arranged to be in a constricted shape to facilitate the quantitative sample to be discharged from the outflow channel 160 through the fluid outlet 170 to the fluid derivation module 300.

[0082] Optionally, refer to Fig.10 The fluid outlet 170 is provided with a quantitative pushing device 171, and the quantitative pushing device 171 includes: a pneumatic microcapsule 172 provided on the inner wall of the fluid outlet, and the pneumatic microcapsule 172 facilitates the sample to fall into the fluid outlet module based on the change of internal air pressure;

[0083] The fluid outlet 170 is provided with a fluid sensor 173 , and the fluid sensor 173 is used to detect and judge the sample of the fluid outlet 170 .

[0084] The fluid outlet 170 is provided with a quantitative pushing device 171. Due to the gravity of the liquid, electrostatic effect and surface tension of the liquid, microliter droplets usually hang on the fluid outlet 170. Additional power is required to push the droplets away from the fluid outlet 170 to propel the microliter of liquid through the constricted outlet and fall into the fluid outlet module 300.

[0085] The quantitative pushing device 171 is a pneumatic microcapsule 172 added to the side wall of the inner wall, which can push the quantitative liquid to break through the resistance such as surface tension and fall into the fluid outlet module 300 through the change of internal air pressure. The pneumatic microcapsule 172 is sealed on the side wall of the fluid outlet 170, and contains a micro device that can cause the air pressure or gas volume in the airbag to change. Generally, the micro device that can cause the air pressure of the airbag to change can be a heating and cooling device, which drives the airbag to move through temperature changes; it can also be connected to an external air pump to provide gas pressure changes.

[0086] The fluid outlet 170 is provided with a fluid sensor 173 , which can determine whether the quantitative liquid-dispensing sample enters the fluid outlet 170 through the second inflow channel 112 , and can also determine whether the liquid-dispensing sample is discharged from the fluid outlet 170 .

[0087] The fluid sensor 173 may be a connected type infiltration sensor, which determines whether there is liquid in the sensing electrode by determining whether the liquid conducts the sensor electrode, thereby determining the liquid state.

[0088] Embodiment 2:

[0089] Reference Fig.11 The difference between this embodiment and the above embodiments is that:

[0090] The fluid channel module is provided with a second inflow channel 112, and the second inflow channel 112 is connected to the outflow channel 160;

[0091] A second fluid introduction valve 122 is provided between the second inflow channel 112 and the outflow channel 160, and a second fluid push-out valve 152 is provided in the outflow channel 160 to control the connection and isolation state between the second inflow channel 112 and the outflow channel 160;

[0092] The second fluid introduction valve 122 and the second fluid discharge valve 152 are controlled by the second driving member and the third driving member, respectively.

[0093] In this embodiment, the second inflow channel 112 is arranged in parallel with the first inflow channel 111, and the second inflow channel 112 is connected to the outflow channel 160. Accordingly, a second fluid introduction valve 122 is also arranged in the second inflow channel 112, and a second fluid ejection valve 152 corresponding to the second fluid introduction valve 122 is also arranged in the outflow channel 160. This embodiment can automatically and quantitatively distribute two different samples, further improving the distribution efficiency.

[0094] Embodiment three:

[0095] Reference Fig.12 The difference between this embodiment and the above embodiments is that:

[0096] The quantitative chamber 140 and the outflow channel 160 are both provided in plurality, and each of the quantitative chamber 140 and the outflow channel 160 is connected to the inflow channel;

[0097] A fluid introduction valve is provided between the inflow channel and each of the quantitative chambers 140, and a fluid push-out valve is provided between each of the quantitative chambers 140 and the outflow channel 160; each of the fluid introduction valves is controlled by the second driving member, and each of the fluid push-out valves is controlled by the third driving member;

[0098] A quantitative push rod 130 is movably disposed in each quantitative chamber 140 .

[0099] In this embodiment, a plurality of quantitative chambers 140 and a plurality of outflow channels 160 are provided, and the plurality of quantitative chambers 140 and the outflow channels 160 are all connected to the inflow channel. A fluid push-out valve is provided in each outflow channel 160, and correspondingly, a plurality of fluid introduction valves corresponding to the fluid push-out valves are also provided in the inflow channel.

[0100] In this embodiment, by providing a plurality of quantitative chambers 140 and outflow channels 160 , the sample can be quantified multiple times at the same time, and then the sample quantified each time is tested at the same time, which further improves the efficiency of automated quantification and testing.

[0101] Embodiment 4:

[0102] Reference Fig.13 The difference between this embodiment and the above embodiments is that:

[0103] The inflow channels are provided in a plurality, and each of the inflow channels is connected to the outflow channel 160; a fluid inlet valve is provided between each of the inflow channels and the outflow channel 160, and a plurality of fluid outlet valves are provided in the outflow channel 160 to control the connection and isolation status of each of the inflow channels and the outflow channel 160.

[0104] In this embodiment, multiple inflow channels are provided, and the multiple inflow channels are provided in parallel with the first inflow channel, and each inflow channel is connected to the outflow channel 160. A fluid introduction valve is provided in each inflow channel, and the outflow channel 160 is also provided with multiple fluid ejection valves corresponding to the fluid introduction valve. This embodiment can automatically and quantitatively distribute a variety of different samples, further improving the distribution efficiency.

[0105] Reference Fig.14 The present invention also provides an automated microliter liquid quantitative dispensing method, comprising the following steps:

[0106] Step 1401: Open the first fluid ejection valve, move the quantitative push rod to the push-in positioning block, and discharge the air in the quantitative chamber from the outflow channel;

[0107] Step 1402: After confirming that the air is exhausted, close the first fluid ejection valve to separate the quantitative chamber from the outflow channel;

[0108] Step 1403: Open the first fluid introduction valve, move the quantitative push rod, and inject the liquid sample in the first inflow channel into the quantitative chamber;

[0109] Step 1404: After the quantitative chamber obtains a quantitative liquid sample from the first inflow channel, the first fluid introduction valve is closed;

[0110] Step 1405: Open the first fluid ejection valve, move the quantitative push rod, and allow the liquid sample to be injected from the quantitative chamber into the outflow channel and discharged from the outflow channel, so that the fluid outlet module can detect the discharged liquid;

[0111] Step 1406: Determine whether the liquid sample has completely flowed out of the outflow channel. If it is confirmed that the liquid sample has not completely flowed out, move the quantitative push rod again to discharge the liquid sample from the outflow channel.

[0112] The automated microliter liquid quantitative distribution method provided in the embodiment of the present invention is achieved by opening a first inflow channel, a quantitative chamber and an outflow channel in a fluid channel module, and respectively arranging a first fluid inlet valve and a first fluid push-out valve in the first inflow channel and the outflow channel, and arranging a quantitative push rod in the quantitative chamber. The working states of the first fluid inlet valve, the first fluid push-out valve and the quantitative push rod are automatically controlled by a driving module to realize automated quantitative distribution of sample liquid, thereby improving the distribution efficiency of the sample.

[0113] Furthermore, before the sample is automatically quantitatively distributed, the air in the quantitative chamber is exhausted, which can effectively prevent the inaccurate volume of the sample quantitative measurement caused by the mixing of air into the sample during the quantitative process.

[0114] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. An automated microliter liquid quantitative dispensing device, characterized in that: include: A fluid channel module, a driving module and a fluid export module; The fluid channel module is provided with a first inflow channel, a quantitative chamber and an outflow channel, and the first inflow channel, the quantitative chamber and the outflow channel are connected; The first inflow channel is provided with a first fluid introduction valve, and the outflow channel is provided with a first fluid push-out valve; a quantitative push rod is movably provided in the quantitative chamber; and the driving module is used to control the working states of the first fluid introduction valve, the first fluid push-out valve and the quantitative push rod; The fluid outlet module is disposed at one end of the outflow channel away from the fluid outlet valve to receive and detect the sample flowing out of the outflow channel; The driving module includes a first driving member, a second driving member and a third driving member; The first driving member is connected to the quantitative push rod to control the position of the quantitative push rod; the second driving member and the third driving member are respectively connected to the first fluid introduction valve and the first fluid push valve to control the opening and closing of the first fluid introduction valve and the first fluid push valve; The fluid channel module is provided with a second inflow channel, and the second inflow channel is connected with the outflow channel; A second fluid introduction valve is provided between the second inflow channel and the outflow channel, and a second fluid push-out valve is provided in the outflow channel to control the connection and isolation state between the second inflow channel and the outflow channel; The second fluid introduction valve and the second fluid ejection valve are controlled by the second driving member and the third driving member respectively; The fluid channel module is connected to a fluid outlet, and the fluid outlet is arranged at the sample outflow end of the outflow channel; The other end of the fluid outlet is connected to the fluid outlet module, and one end of the fluid outlet connected to the fluid outlet module is in a constricted shape.

2. An automated microliter liquid quantitative dispensing device according to claim 1, characterized in that: The quantitative chamber and the outflow channel are both provided in plurality, and each of the quantitative chamber and the outflow channel is communicated with the inflow channel; A fluid introduction valve is provided between the inflow channel and each of the quantitative chambers, and a fluid push-out valve is provided between each of the quantitative chambers and the outflow channel; each of the fluid introduction valves is controlled by the second driving member, and each of the fluid push-out valves is controlled by the third driving member; A quantitative push rod is movably arranged in each quantitative chamber.

3. An automated microliter liquid quantitative dispensing device according to claim 1 or 2, characterized in that: The two ends of the quantitative push rod are respectively provided with a first limit plate and a second limit plate, the first limit plate extends into the quantitative chamber, and the second limit plate is located outside the quantitative chamber and connected to the first driving member; The inner wall of the quantitative chamber is provided with a push-out positioning block and a push-in positioning block, and the quantitative push rod moves between the push-out positioning block and the push-in positioning block; A position feedback sensor is provided at one end of the quantitative push rod extending into the quantitative chamber, and the position feedback sensor is used to feedback the pushing stroke of the quantitative push rod by the first driving member.

4. The automatic microliter liquid quantitative dispensing device according to claim 3, characterized in that: The position feedback sensor comprises: an electrode arranged on the side of the quantitative push rod; A resistor is disposed on the side wall of the quantitative chamber, and the resistor is connected to the electrode to determine the position of the quantitative push rod pushed by the first driving member.

5. The automatic microliter liquid quantitative dispensing device according to claim 4, characterized in that: A sealing member is provided at one end of the quantitative push rod close to the first limit plate, and the sealing member is provided at the periphery of the quantitative push rod. One end of the sealing member is connected to the second limit plate, and the other end of the sealing member is connected to the third limit plate, and the third limit plate is fixedly connected to the rod body of the quantitative push rod.

6. The automatic microliter liquid quantitative dispensing device according to claim 1, characterized in that: A quantitative pushing device is arranged in the fluid outlet, and the quantitative pushing device comprises: a pneumatic microcapsule arranged on the inner wall of the fluid outlet, and the pneumatic microcapsule facilitates the sample to fall into the fluid outlet module based on the change of internal air pressure; The fluid outlet is provided with a fluid sensor, and the fluid sensor is used to detect and judge the sample of the fluid outlet.

7. An automated liquid quantitative dispensing and detection method, characterized in that: Applied to an automated microliter liquid quantitative dispensing device as claimed in any one of claims 1 to 6, the method comprising: Open the first fluid ejection valve, move the quantitative push rod to the push-in positioning block, and discharge the air in the quantitative chamber from the outflow channel; After confirming that the air is discharged, closing the first fluid ejection valve to separate the quantitative chamber from the outflow channel; Opening the first fluid introduction valve, moving the quantitative push rod, and injecting the liquid sample in the first inflow channel into the quantitative chamber; After the quantitative chamber obtains a quantitative liquid sample from the first inflow channel, the first fluid introduction valve is closed; Opening the first fluid ejection valve, moving the quantitative push rod, allowing the liquid sample to be injected from the quantitative chamber into the outflow channel, and discharged from the outflow channel, so that the fluid outlet module can detect the discharged liquid; It is determined whether the liquid sample has completely flowed out of the outflow channel. If it is confirmed that the liquid sample has not completely flowed out, the quantitative push rod is moved again or the pneumatic microcapsule is pushed to discharge the liquid sample from the outflow channel.

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