A multi-item testing machine

Abstract

The present invention discloses a multi-item detection integrated machine, which includes an electrochemical detection mechanism, a high pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used to detect the content of blood sugar and fructosamine, and the high pressure liquid chromatography mechanism is used to detect the content of glycosylated hemoglobin; the sample adding mechanism is connected to the high pressure liquid chromatography mechanism, and the sample adding mechanism is used to transport the sample solution to the high pressure liquid chromatography mechanism and drip the sample solution into the electrochemical detection mechanism. In the present invention, by integrating the electrochemical detection mechanism and the high pressure liquid chromatography mechanism, the multi-item detection integrated machine can detect blood sugar, fructosamine and glycosylated hemoglobin at the same time, thereby avoiding the need for three detection items to be performed on three different instrument platforms, improving the detection efficiency, and also avoiding the need for multiple blood draws on patients when detecting different items.

Description

Technical Field

[0001] The present invention relates to the technical field of biological indicator detection, and in particular to a multi-item detection integrated machine. Background Art

[0002] Currently, the prevalence and incidence of diabetes are rising sharply around the world, and there are many means of detecting diabetes, each with its own advantages and disadvantages. Generally speaking, blood sugar is the most important experimental test item in the diagnosis and treatment of diabetes. Blood sugar reflects the instantaneous level of glucose in the patient's body, but blood sugar is easily affected by many factors such as diet, drugs, and emotions, and sometimes cannot objectively reflect the long-term level of glucose in the human body. Fructosamine reflects the average level of blood sugar in the patient 2 to 3 weeks before the measurement. Fructosamine is more sensitive to short-term blood sugar changes than HbA1c. It is a good indicator for evaluating the patient's short-term glucose metabolism control, especially for the evaluation of the efficacy of diabetic patients after the treatment plan is adjusted. However, the specificity of some fructosamine detection methods is not high, and there is interference from urate and hyperlipidemia. Glycated hemoglobin (HbA1c) is the product of hemoglobin in red blood cells combined with glucose. It is formed through a slow, continuous and irreversible glycation reaction. Its content depends on the blood sugar concentration and the contact time between blood sugar and hemoglobin, and has nothing to do with factors such as the time of blood draw, whether the patient is fasting, and whether insulin is used. HbA1c can effectively reflect the blood sugar control of diabetic patients in the past 2 to 3 months. However, glycated hemoglobin cannot determine the level of blood sugar control of patients in the short term, nor can it monitor the effect of medication in the short term.

[0003] For the above reasons, in clinical tests, it is generally necessary to conduct a joint test of the above three indicators to assist in judgment. However, at present, the tests of blood sugar, fructosamine, and glycosylated hemoglobin need to be performed on different testing platforms. For example, blood sugar needs to be tested on a biochemical analyzer, and glycosylated hemoglobin needs to be tested on a glycosylated hemoglobin analyzer based on HPLC technology, so the detection efficiency is low; moreover, the sample characteristics of blood sugar test are serum and plasma, while the sample characteristics of glycosylated hemoglobin are whole blood, which requires multiple blood draws from patients.

[0004] Therefore, the prior art still needs to be improved and developed. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a multi-item integrated testing machine in view of the shortcomings of the prior art, so as to solve the problem that the tests of blood glucose, fructosamine and glycosylated hemoglobin in the prior art need to be tested on different platforms and that the patient needs to undergo multiple blood draws during the tests.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0007] A multi-item detection integrated machine, comprising an electrochemical detection mechanism, a high pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used to detect the content of blood sugar and fructosamine, and the high pressure liquid chromatography mechanism is used to detect the content of glycated hemoglobin;

[0008] The sample adding mechanism is connected to the high pressure liquid chromatography mechanism, and is used to transport the sample solution to the high pressure liquid chromatography mechanism and drip the sample solution into the electrochemical detection mechanism.

[0009] The multi-item detection integrated machine, wherein the sample loading mechanism includes a solenoid valve group, a syringe, a sample loading needle, a hemolysis pool and a solution tank, the solution tank contains a hemolytic agent or a cleaning agent, and the syringe, the sample loading needle, the solution tank and the hemolysis pool are all connected to the solenoid valve group through pipelines; by switching the solenoid valve group, the syringe is connected to the sample loading needle, or the syringe is connected to the solution tank, or the syringe is connected to the hemolysis pool.

[0010] The multi-item detection integrated machine, wherein the solenoid valve group includes a first solenoid valve, a second solenoid valve and a third solenoid valve, a first pipeline is connected between the first solenoid valve and the first solution tank, a second pipeline is connected between the second solenoid valve and the sample addition needle, and a third pipeline is connected between the third solenoid valve and the hemolysis pool;

[0011] The syringe is connected to a fourth pipeline, and the first solenoid valve, the second solenoid valve and the third solenoid valve are all connected to an end of the fourth pipeline away from the syringe.

[0012] The multi-item detection integrated machine, wherein the solenoid valve group further includes a fourth solenoid valve, the sample adding needle is provided with a cleaning component, and a fifth pipeline is connected between the fourth solenoid valve and the cleaning component;

[0013] The syringe fills the cleaning fluid into the cleaning part through the fifth pipeline to clean the blood on the sample addition needle.

[0014] The multi-item testing integrated machine, wherein the bottom of the hemolysis pool is connected to a waste liquid pool.

[0015] The multi-item testing machine, wherein the high pressure liquid chromatography mechanism comprises a high pressure pump, a sample loop and a glycosylated hemoglobin testing device connected in sequence;

[0016] The sample loop is connected to the second pipeline, and the syringe draws the sample solution into the sample loop by switching to communicate with the second pipeline.

[0017] The multi-item detection integrated machine, wherein the glycated hemoglobin detection device comprises a chromatography column and a detection module, and the sample loop, the chromatography column and the detection module are connected in sequence.

[0018] The multi-item detection integrated machine, wherein the high pressure liquid chromatography mechanism further comprises a sample valve, on which a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a sixth valve are sequentially arranged, and a first passage is connected between the first valve and the second valve, a second passage is connected between the second valve and the third valve, a third passage is connected between the third valve and the fourth valve, a fourth passage is connected between the fifth valve and the sixth valve, and a fifth passage is connected between the sixth valve and the first valve;

[0019] The second pipeline includes a first pipe section and a second pipe section, one end of the first pipe section is connected to the second solenoid valve, and the other end is connected to the first valve, and one end of the second pipe section is connected to the second valve, and the other end is connected to the sample injection needle;

[0020] A first high-pressure pipeline is connected between the high-pressure pump and the fifth valve, a first loop is connected between the sixth valve and the sample ring, a second loop is connected between the sample ring and the third valve, and a second high-pressure pipeline is connected between the fourth valve and the glycated hemoglobin detection device.

[0021] The multi-item detection integrated machine, wherein the electrochemical detection mechanism includes a sample flow cell and an electrolytic cell, the sample flow cell is located at the bottom of the electrolytic cell, and the sample flow cell is connected to the electrolytic cell through an oxygen semipermeable membrane, and glucose oxidase or ketoaminase is added to the sample flow cell; the distance between the cathode in the electrolytic cell and the oxygen semipermeable membrane is smaller than the distance between the anode in the electrolytic cell and the oxygen semipermeable membrane.

[0022] The multi-item detection integrated machine, wherein the cathode is platinum, the anode is Ag or AgCl, and a polarization voltage of 0.6V-0.8V is applied between the cathode and the anode.

[0023] Beneficial effect: In the present invention, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can detect blood glucose, fructosamine and glycosylated hemoglobin at the same time, thereby avoiding the need to perform three detection items on three different instrument platforms, improving the detection efficiency, and also avoiding the need to perform multiple blood sampling operations on patients when detecting different items. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1A schematic diagram of the structure of the multi-item detection integrated machine (excluding the electrochemical detection mechanism) provided by the present invention;

[0025] Figure 2 A schematic diagram of the structure of the electrochemical detection mechanism provided by the present invention;

[0026] The markings in the attached drawings are: 1, syringe; 2, sample injection needle; 3, hemolysis pool; 4, solenoid valve group; 41, first solenoid valve; 42, second solenoid valve; 43, third solenoid valve; 44, fourth solenoid valve; 5, solution tank; 6, first pipeline; 7, second pipeline; 71, first pipe section; 72, second pipe section; 8, third pipeline; 9, fourth pipeline; 10, fifth pipeline; 11, waste liquid pool; 12, high pressure pump; 13, sample loop; 14, glycated hemoglobin detection device; 141, chromatography column; 142, detection module block; 15, sample valve; 16, first valve; 17, second valve; 18, third valve; 19, fourth valve; 20, fifth valve; 21, sixth valve; 22, first passage; 23, second passage; 24, third passage; 25, fourth passage; 26, fifth passage; 27, first high-pressure pipeline; 28, first loop; 29, second loop; 30, second high-pressure pipeline; 31, sample flow cell; 32, electrolytic cell; 33, oxygen semipermeable membrane; 34, cathode; 35, anode; 36, cleaning parts. DETAILED DESCRIPTION

[0027] The present invention provides a multi-item detection integrated machine. In order to make the purpose, technical solution and effect of the present invention clearer and more specific, the present invention is further described in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention and are not used to limit the present invention.

[0028] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it can be directly on the other component or indirectly on the other component. When a component is referred to as being "connected to" another component, it can be directly connected to the other component or indirectly connected to the other component.

[0029] It should also be noted that the same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, it is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as limitations on this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0030] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0031] The invention is further described below by describing embodiments in conjunction with the accompanying drawings.

[0032] The present embodiment provides a multi-item detection integrated machine, which includes an electrochemical detection mechanism, a high pressure liquid chromatography mechanism and a sample loading mechanism, wherein the sample loading mechanism is connected to the high pressure liquid chromatography mechanism, the electrochemical detection mechanism is used to detect the content of blood glucose and fructosamine, and the high pressure liquid chromatography mechanism is used to detect the content of glycated hemoglobin; the sample loading mechanism can obtain a blood sample, and can obtain a sample solution by dissolving a hemolytic agent with the blood sample; after obtaining the sample solution, the sample loading mechanism is also used to transport the sample solution to the high pressure liquid chromatography mechanism, so as to detect the content of glycated hemoglobin in the sample solution through the high pressure liquid chromatography mechanism, and the sample loading mechanism can also drip the sample solution into the electrochemical detection mechanism located on one side, so as to detect the content of blood glucose and fructosamine in the sample solution through the electrochemical detection mechanism.

[0033] In the present invention, the electrochemical detection mechanism and the high pressure liquid chromatography mechanism are integrated together, so that the multi-item detection integrated machine can detect blood glucose, fructosamine and glycosylated hemoglobin at the same time, thereby avoiding the need to perform the three detection items on three different instrument platforms, improving the detection efficiency, and also avoiding the need to perform multiple blood draws on patients when detecting different items.

[0034] Specifically, Figure 1As shown, the loading mechanism includes an electromagnetic valve group 4, a syringe 1, a loading needle 2, a hemolyzing pool 3 and a solution tank 5, wherein the solution tank 5 is used to contain a hemolyzing agent or a cleaning agent, and the syringe 1, the loading needle 2, the solution tank 5 and the hemolyzing pool 3 are all connected to the electromagnetic valve group 4 through a pipeline; by switching the electromagnetic valve group 4, the syringe 1 can be connected to the loading needle 2, or the syringe 1 can be connected to the solution tank 5, or the syringe 1 can be connected to the hemolyzing pool 3, that is, the syringe can be connected to one of the loading needle 2, the hemolyzing pool 3 and the solution tank 5 through the switching of the electromagnetic valve group. When the sample solution needs to be generated, the solenoid valve group 4 first switches to the syringe 1 to be connected to the sample needle, the syringe 1 sucks the blood sample into the sample needle 2, and then the syringe 1 adds the blood sample in the sample needle 2 to the hemolysis pool 3, and then the solenoid valve group 4 switches again to connect the syringe 1 to the solution tank 5, the syringe 1 sucks the hemolytic agent in the solution tank 5, and then the solenoid valve group 4 switches to the syringe 1 to be connected to the hemolysis pool 3, the syringe 1 discharges the sucked hemolytic agent into the hemolysis pool 3 to mix with the blood sample in the hemolysis pool 3, so as to dissolve the hemoglobin in the red blood cells in the blood sample, and finally form the sample solution. Further, when sample addition detection is required, the solenoid valve group 4 switches to the syringe 1 to be connected to the sample needle 2, and sucks the sample solution into the high pressure liquid chromatography mechanism through the syringe 1, or sucks and drips the sample solution into the electrochemical detection mechanism.

[0035] Furthermore, the solenoid valve group 4 includes a first solenoid valve 41, a second solenoid valve 42 and a third solenoid valve 43, a first pipeline 6 is connected between the first solenoid valve 41 and the solution tank 5; a second pipeline 7 is connected between the second solenoid valve 42 and the sample addition needle 2, and a third pipeline 8 is connected between the third solenoid valve 43 and the hemolysis pool 3; the syringe 1 is connected to a fourth pipeline 9, and the first solenoid valve 41, the second solenoid valve 42 and the third solenoid valve 43 are all connected to the end of the fourth pipeline 9 away from the syringe 1. When the first solenoid valve 41 is switched to the fourth pipeline 9 and connected to the first pipeline 6, the syringe 1 can draw the hemolytic agent from the solution tank 5; when the second solenoid valve 42 is switched to the fourth pipeline 9 and connected to the second pipeline 7, the syringe 1 can suck the blood sample into the sampling needle 2, or push the blood sample from the sampling needle 2 into the hemolysis pool 3; when the third solenoid valve 43 is switched to the fourth pipeline 9 and connected to the third pipeline 8, the syringe 1 can add the hemolytic agent sucked into the syringe 1 to the hemolysis pool 3, and after adding the hemolytic agent to the blood sample in the hemolysis pool 3, the liquid in the hemolysis pool 3 can be continuously sucked into the third pipeline 8, and discharged from the third pipeline 8 to the hemolysis pool 3 in time, and the hemoglobin in the hemolysis pool 3 can be more completely dissolved through continuous suction and discharge actions.

[0036] The solenoid valve group 4 also includes a fourth solenoid valve 44, a cleaning component 36 is provided on the sample addition needle 2, and a fifth pipeline 10 is connected between the fourth solenoid valve 44 and the cleaning component 36; since some blood will inevitably remain on the outer wall of the end of the sample addition needle 2 after the sample addition needle 2 absorbs the blood sample, in order to refresh the sample addition needle 2, the syringe 1 fills the cleaning liquid into the cleaning component 36 through the fifth pipeline 10 to clean the blood on the sample addition needle 2, that is, the first solenoid valve 41 is first switched to the first pipeline 6 to be connected to the fourth pipeline 9, the syringe 1 absorbs the cleaning liquid from the solution tank 5, and then the fourth solenoid valve 44 is switched to the fifth pipeline 10 to be connected to the fourth pipeline 9, and the syringe 1 pushes the cleaning liquid into the cleaning component 36 to clean the blood on the sample addition needle 2.

[0037] The bottom of the hemolysis pool 3 is connected to a waste liquid pool 11 so that the remaining liquid in the hemolysis pool 3 can be discharged into the waste liquid pool 11 after the detection of various biological indicators is completed.

[0038] The high pressure liquid chromatography mechanism includes a high pressure pump 12, a sample ring 13 and a glycated hemoglobin detection device 14 which are connected in sequence; the sample ring 13 is connected to the second pipeline 7, and the syringe 1 draws the sample solution into the sample ring 13 by switching to connect to the second pipeline 7; specifically, when it is necessary to detect the content of glycated hemoglobin, the second solenoid valve 42 switches to the fourth pipeline 9 to connect to the second pipeline 7, the syringe 1 draws the sample solution from the dissolution tank into the sample ring 13, and then the high pressure pump 12 pushes the sample solution in the sample ring 13 into the glycated hemoglobin detection device 14 for detection.

[0039] The glycated hemoglobin detection device 14 includes a chromatography column 141 and a detection module 142. The sample ring 13, the chromatography column 141 and the detection module 142 are connected in sequence. When the sample solution is pushed into the chromatography column 141 by the eluent pumped by the high-pressure pump 12, the sample solution is chromatographed through the chromatography column 141, and the chromatographed liquid enters the detection module 142 for detection to obtain the glycated hemoglobin content. Furthermore, the detection module 142 can be connected to the waste liquid pool 11 to discharge the liquid after the glycated hemoglobin detection into the waste liquid pool 11.

[0040] The high pressure liquid chromatography mechanism further comprises a sample valve 15, on which a first valve 16, a second valve 17, a third valve 18, a fourth valve 19, a fifth valve 20 and a sixth valve 21 are sequentially arranged, and a first passage 22 is connected between the first valve 16 and the second valve 17, a second passage 23 is connected between the second valve 17 and the third valve 18, a third passage 24 is connected between the third valve 18 and the fourth valve 19, a fourth passage 25 is connected between the fifth valve 20 and the sixth valve 21, and a fifth passage 26 is connected between the sixth valve 21 and the first valve 16. 6; the second pipeline 7 includes a first pipe section 71 and a second pipe section 72, one end of the first pipe section 71 is connected to the second solenoid valve 42, and the other end is connected to the first valve 16, one end of the second pipe section 72 is connected to the second valve 17, and the other end is connected to the sample adding needle 2; a first high-pressure pipeline 27 is connected between the high-pressure pump 12 and the fifth valve 20, a first loop 28 is connected between the sixth valve 21 and the sample ring 13, a second loop 29 is connected between the sample ring 13 and the third valve 18, and a second high-pressure pipeline 30 is connected between the fourth valve 19 and the glycated hemoglobin detection device 14.

[0041] By switching the first valve 16, the second valve 17, the third valve 18, the fourth valve 19, the fifth valve 20 and the sixth valve 21, the communication between the sample loop 13 and the second pipeline 7, the communication between the sample loop 13 and the high-pressure pump 12, or the communication between the first passage 22 and the second pipeline 7 can be achieved. Specifically, when the sampling needle 2 is needed to obtain a blood sample or add a blood sample to the hemolysis pool 3, the second battery valve is switched to connect the fourth pipeline 9 to the second pipeline 7, and the first valve 16 and the second valve 17 are switched to connect the first pipe section 71, the first passage 22 and the second pipe section 72 in sequence, so that the blood is sucked into the sampling needle 2 through the syringe 1, or the blood in the sampling needle 2 is discharged into the hemolysis pool 3; when the sampling needle 2 is needed to suck the sample solution in the hemolysis pool 3 into the sample ring 13, the second battery valve is switched to connect the fourth pipeline 9 to the second pipeline 7, and the first valve 16, the second valve 17, the third valve 18 and the sixth valve 21 are switched to connect the first pipe section 71, The fifth passage 26, the first loop 28, the second loop 29, the second passage 23 and the second pipe section 72 are connected in sequence to suck the sample solution from the solution pool into the sample loop 13 through the syringe 1 and the sample adding needle 2; further, when the sample solution in the sample loop 13 needs to be transported to the glycated hemoglobin detection device 14, the third valve 18, the fourth valve 19, the fifth valve 20 and the sixth valve 21 are switched to connect the first high-pressure pipeline 27, the fourth passage 25, the first loop 28, the second loop 29, the third passage 24 and the second high-pressure pipeline 30 in sequence, so that the high-pressure pump 12 pumps the eluent into the sample loop 13, and pushes the sample solution in the sample loop 13 into the glycated hemoglobin detection device 14.

[0042] Since the first high-pressure pipeline 27 and the second high-pressure pipeline 30 usually have relatively high pressure, and the second pipeline 7 is a vacuum pipeline, the sample valve 15 is provided to switch the pipelines, thereby isolating the pressure.

[0043] like Figure 2As shown, the electrochemical detection mechanism includes a sample flow cell 31 and an electrolytic cell 32, wherein the sample flow cell 31 is located at the bottom of the electrolytic cell 32, and the sample flow cell 31 is connected to the electrolytic cell 32 through an oxygen semipermeable membrane 33, and glucose oxidase or ketoaminase is added to the sample flow cell 31; the distance between the cathode 34 in the electrolytic cell 32 and the oxygen semipermeable membrane 33 is smaller than the distance between the anode 35 in the electrolytic cell 32 and the oxygen semipermeable membrane 33, that is, the cathode 34 is arranged close to the oxygen semipermeable membrane 33, and the anode 35 is arranged away from the oxygen semipermeable membrane 33. In a specific embodiment, the cathode 34 is platinum, the anode 35 is Ag or AgCl, and a polarization voltage of 0.6V-0.8V is applied between the cathode 34 and the anode 35. In the platinum--Ag / AgCl electrolytic cell 32, the area of ​​the platinum cathode 34 is very small, which is tens to hundreds of times the area of ​​the Ag / AgCl anode 35. A stable polarization voltage of 0.6--0.8V is applied between the platinum--Ag / AgCl electrodes. The oxygen in the sample flow cell 31 passes through the oxygen semipermeable membrane 33 to the surface of the cathode 34 and is reduced, thereby generating a redox reaction. The reduction reaction formula is as follows: O2+4e+2H2O------4OH-, generating an electrolytic current, so that the oxygen concentration on the surface of the cathode 34 is much lower than that on the surface of the anode 35. At this time, concentration diffusion occurs. When the diffusion concentration is relatively stable, a stable electrolytic current is generated, also called the limiting diffusion current. The magnitude of this current is determined by the amount of oxygen that penetrates into the surface of the cathode 34, and the amount of oxygen that penetrates into the surface of the cathode 34 is determined by the amount of oxygen in the sample flow cell 31.

[0044] For example, when it is necessary to detect the content of blood sugar or fructosamine in the sample solution, the second battery valve is switched to connect the fourth pipeline 9 with the second pipeline 7, and the first valve 16 and the second valve 17 are switched to connect the first pipe section 71, the first passage 22 and the second pipe section 72 in sequence, and then the sample solution is sucked into the sample injection needle 2 through the syringe 1, and then the sample injection needle 2 is moved above the flow cell, and the sample solution is dripped into the sample flow cell 31, and when the glucose oxidase is added to the sample flow cell 31, the blood sugar consumes oxygen under the catalysis of the glucose oxidase, wherein the reaction formula of glucose under the catalysis of GOD (glucose oxidase) is:

[0045]

[0046] Blood sugar consumes oxygen under the catalysis of glucose oxidase, and the oxygen in the sample flow cell 31 will decrease. At this time, oxygen concentration diffusion will occur, causing the oxygen in the electrolytic cell 32 to diffuse into the sample flow cell 31 through the oxygen semipermeable membrane 33, thereby further causing the oxygen content in the electrolytic cell 32 to decrease, especially the oxygen required for the reduction reaction to be supplied to the cathode 34 is reduced, so the electrolysis current also becomes weaker, that is, the electrolysis current changes; by measuring the size of the current before and after the change between the cathode 34 and the anode 35, the change in oxygen content in the electrolytic solution can be detected, and after obtaining the change in oxygen content, the blood sugar content can be further calculated based on the catalytic principle of blood sugar being catalysted by glucose oxidase.

[0047] The fructosamine content is detected by adding the ketoaminase as a catalyst into the sample flow cell 31 to consume the oxygen in the sample flow cell 31, so that the change in oxygen content can be obtained according to the change in the electrolysis current, and the fructosamine content can be further obtained.

[0048] The multi-item detection integrated machine also includes a control system, which controls the switching of each valve in the sample ring 13, the switching of the solenoid valve group 4, the movement of the sample injection needle 2, and the operation of the syringe 1, the electrochemical reaction mechanism and the high pressure liquid chromatography mechanism.

[0049] In summary, the present invention discloses a multi-item detection integrated machine, which includes an electrochemical detection mechanism, a high pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used to detect the content of blood sugar and fructosamine, and the high pressure liquid chromatography mechanism is used to detect the content of glycated hemoglobin; the sample adding mechanism is connected to the high pressure liquid chromatography mechanism, and the sample adding mechanism is used to transport the sample solution to the high pressure liquid chromatography mechanism and drip the sample solution into the electrochemical detection mechanism. In the present invention, by integrating the electrochemical detection mechanism and the high pressure liquid chromatography mechanism together, the multi-item detection integrated machine can detect blood sugar, fructosamine and glycated hemoglobin at the same time, thereby avoiding the need for the three detection items to be performed on three different instrument platforms, improving the detection efficiency, and also avoiding the need for multiple blood draws on patients when detecting different items.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-item testing machine, characterized in that: It includes an electrochemical detection mechanism, a high pressure liquid chromatography mechanism and a sample adding mechanism, wherein the electrochemical detection mechanism is used to detect the content of blood sugar and fructosamine, and the high pressure liquid chromatography mechanism is used to detect the content of glycated hemoglobin; the sample adding mechanism is connected to the high pressure liquid chromatography mechanism, and the sample adding mechanism is used to transport the sample solution to the high pressure liquid chromatography mechanism and drip the sample solution into the electrochemical detection mechanism; The sample adding mechanism comprises a solenoid valve group, a syringe, a sample adding needle, a hemolysis pool and a solution tank, wherein the solution tank contains a hemolytic agent or a cleaning agent, and the syringe, the sample adding needle, the solution tank and the hemolysis pool are all connected to the solenoid valve group through pipelines; the syringe is connected to the sample adding needle, or the syringe is connected to the solution tank, or the syringe is connected to the hemolysis pool by switching the solenoid valve group; The solenoid valve group includes a first solenoid valve, a second solenoid valve and a third solenoid valve, a first pipeline is connected between the first solenoid valve and the solution tank, a second pipeline is connected between the second solenoid valve and the sample addition needle, and a third pipeline is connected between the third solenoid valve and the hemolysis pool; the syringe is connected to a fourth pipeline, and the first solenoid valve, the second solenoid valve and the third solenoid valve are all connected to an end of the fourth pipeline away from the syringe; The solenoid valve group further includes a fourth solenoid valve, a cleaning component is provided on the sample addition needle, and a fifth pipeline is connected between the fourth solenoid valve and the cleaning component; the syringe fills the cleaning component with cleaning liquid through the fifth pipeline to clean the blood on the sample addition needle; The high pressure liquid chromatography mechanism comprises a high pressure pump, a sample loop and a glycated hemoglobin detection device which are connected in sequence; the sample loop is connected to the second pipeline, and the syringe draws the sample solution into the sample loop by switching to connect to the second pipeline; The glycated hemoglobin detection device comprises a chromatography column and a detection module, wherein the sample loop, the chromatography column and the detection module are connected in sequence; The high pressure liquid chromatography mechanism also includes a sample valve, on which a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a sixth valve are sequentially arranged, and a first passage is connected between the first valve and the second valve, a second passage is connected between the second valve and the third valve, a third passage is connected between the third valve and the fourth valve, a fourth passage is connected between the fifth valve and the sixth valve, and a fifth passage is connected between the sixth valve and the first valve; the second pipeline includes a first pipe section and a second pipe section, one end of the first pipe section is connected to the second solenoid valve, and the other end is connected to the first valve, one end of the second pipe section is connected to the second valve, and the other end is connected to the sample needle; a first high pressure pipeline is connected between the high pressure pump and the fifth valve, a first loop is connected between the sixth valve and the sample ring, a second loop is connected between the sample ring and the third valve, and a second high pressure pipeline is connected between the fourth valve and the glycated hemoglobin detection device; when the sample needle is used to obtain a blood sample or to add a blood sample to the hemolysis pool, the second battery valve is switched to the fourth pipeline to communicate with the second pipeline At the same time, the first valve and the second valve are switched to connect the first pipe section, the first passage and the second pipe section in sequence, so that the blood is sucked into the sampling needle through the syringe, or the blood in the sampling needle is discharged into the hemolysis pool; when the sampling needle is used to suck the sample solution in the hemolysis pool into the sample loop, the second battery valve is switched to connect the fourth pipeline with the second pipeline, and the first valve, the second valve, the third valve and the sixth valve are switched to connect the first pipe section, the fifth passage, the first loop, the second loop, the The second passage and the second pipe section are connected in sequence, so that the sample solution is sucked from the solution pool into the sample loop through the syringe and the sample injection needle; when the sample solution in the sample loop needs to be transported to the glycated hemoglobin detection device, the third valve, the fourth valve, the fifth valve and the sixth valve are switched to connect the first high-pressure pipeline, the fourth passage, the first loop, the second loop, the third passage and the second high-pressure pipeline in sequence, so that the high-pressure pump pumps the eluent into the sample loop, and pushes the sample solution in the sample loop into the glycated hemoglobin detection device; The electrochemical detection mechanism includes a sample flow cell and an electrolytic cell, wherein the sample flow cell is located at the bottom of the electrolytic cell and is connected to the electrolytic cell through an oxygen semipermeable membrane, and glucose oxidase or ketoaminase is added to the sample flow cell; the distance between the cathode in the electrolytic cell and the oxygen semipermeable membrane is smaller than the distance between the anode in the electrolytic cell and the oxygen semipermeable membrane; when it is necessary to detect the content of blood glucose or fructoseamine in the sample solution, the first valve and the second valve need to be switched while the second battery valve is switched to connect the fourth pipeline with the second pipeline, so that the first pipe section, the first passage and the second pipe section are connected in sequence, and then the sample solution is sucked into the sample injection needle through the syringe, and then the The sample addition needle moves to the top of the flow cell and drops the sample solution into the sample flow cell. When the glucose oxidase is added to the sample flow cell, blood glucose consumes oxygen under the catalysis of the glucose oxidase. The change in oxygen content in the electrolytic solution can be detected by measuring the magnitude of the current between the cathode and the anode before and after the change. After obtaining the change in oxygen content, the blood glucose content can be further calculated based on the catalytic principle of blood glucose being catalyzed by the glucose oxidase catalyst. The detection of the fructosamine content is to add the ketoamine enzyme as a catalyst to the sample flow cell to consume the oxygen in the sample flow cell, so that the change in oxygen content can also be obtained based on the change in the electrolysis current, and the fructosamine content can be further obtained.

2. The multi-item detection integrated machine according to claim 1, characterized in that: The bottom of the hemolysis pool is connected with a waste liquid pool.

3. The multi-item detection integrated machine according to claim 1, characterized in that: The cathode is platinum, the anode is Ag or AgCl, and a polarization voltage of 0.6V-0.8V is applied between the cathode and the anode.

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