An intelligent plant pathogenic bacteria culture system and its culture method

Abstract

The present invention discloses an intelligent plant pathogenic bacteria culture system and a culture method in the field of plant bacteria culture, which includes a support column fixedly arranged on a frame, and a light source is arranged on the support column; a carrying platform is rotatably installed at intervals along the axial direction of the support column, a loading platform is arranged on the carrying platform, a sliding groove is vertically opened on the carrying platform, and the loading platform is vertically slidably installed on the sliding groove; a light regulating mechanism is arranged beside the loading platform. This culture system can not only automatically block the circumferential side wall of the culture dish to prevent external light from entering the culture dish through the circumferential side wall of the culture dish; but also reflect the external light to the center of the culture dish through a reflector, so that plant bacteria can reproduce in the middle of the culture dish, facilitating observation. In addition, the present invention can also automatically adjust the reflection angle of the reflector according to the liquid level height in the culture dish, so that the reflector can always reflect the light to the center of the liquid surface of the culture dish, facilitating subsequent observation and improving the efficiency of the experiment.

Description

Technical Field

[0001] The present invention relates to the field of plant bacteria culture, and particularly to an intelligent plant pathogenic bacteria culture system and a culture method thereof. Background Art

[0002] If the fungal mycelium in the diseased plant tissue is given suitable environmental conditions, generally it can resume growth and reproduction except for individual species. The isolation of plant pathogens refers to separating the pathogenic fungi from other miscellaneous bacteria in the diseased plant tissue through artificial culture, isolating them from the host plant, and then purifying the isolated pathogens in a suitable environment. This process is generally called the isolation and culture of plant pathogens. The isolation of plant pathogenic fungi generally adopts the tissue isolation method, that is, cutting small pieces of diseased tissue, after surface disinfection and washing with sterile water, transferring them to an artificial culture medium for culture. Usually, the culture dish carrying the culture medium is made of glass, which has light transmittance and is convenient for experimenters to observe the cultivation state in the culture dish at any time during the experiment. However, in the actual culture process of some plant bacteria with phototaxis, because the light source is outside the culture dish, the light emitted by the light source will enter the culture dish through the side wall of the culture dish, and the plant bacteria will adhere to the wall due to phototaxis. In this way, the plant bacteria will multiply in large numbers on the inner side wall of the culture dish, making it extremely inconvenient for subsequent experimenters to observe and affecting the efficiency of the experiment and the accuracy of the observation.

[0003] The present invention provides an intelligent plant pathogenic bacteria culture system and a culture method thereof. This culture system can not only automatically block the circumferential side wall of the culture dish to prevent external light from entering the culture dish through the circumferential side wall of the culture dish, resulting in the phototactic adhesion of plant bacteria to the wall; but also reflect the external light to the center of the culture dish through a reflector, enabling the plant bacteria to reproduce in the middle of the culture dish for easy observation. In addition, the present invention can also automatically adjust the reflection angle of the reflector according to the liquid level height in the culture dish, so that the reflector can always reflect the light to the center of the liquid surface in the culture dish, facilitating subsequent observation and improving the efficiency of the experiment. Summary of the Invention

[0004] The purpose of the present invention is to provide an intelligent plant pathogenic bacteria culture system and a culture method thereof to solve the problems of the disadvantages of the prior art mentioned in the above background art.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an intelligent plant pathogenic bacteria culture system, comprising a support column fixedly arranged on a frame, wherein a light source is arranged on the support column; a bearing platform is rotatably installed along the axial direction of the support column, wherein a loading platform for placing a culture dish is arranged on the bearing platform, wherein a sliding groove is vertically opened on the bearing platform, wherein the loading platform is vertically slidably installed on the sliding groove, wherein a first compression spring is arranged between the loading platform and the bottom of the sliding groove; a dimming mechanism is arranged on the side of the loading platform, wherein the dimming mechanism comprises a reflector, wherein the reflector is used to reflect the light emitted by the light source to the center of the liquid surface of the culture dish; and the dimming mechanism adjusts the reflection angle of the reflector according to the displacement of the loading platform.

[0006] As a further solution of the present invention, the dimming mechanism includes a support rod fixedly installed next to the loading platform, a rack is fixedly installed at the bottom edge of the loading platform, the rack is located next to the support rod, a first rotating shaft is rotatably installed at the lower end of the support rod, and a second rotating shaft is rotatably installed at the upper end; a gear and a first synchronous wheel are fixedly installed on the first rotating shaft, and the gear is meshed with the rack; a second synchronous wheel is fixedly installed on the second rotating shaft, and the first synchronous wheel is synchronously connected with the second synchronous wheel; the reflector is fixedly installed on the second rotating shaft.

[0007] As a further solution of the present invention, the reflector is a convex mirror.

[0008] As a further solution of the present invention, a guiding mechanism is arranged in the circumference of the stage; the guiding mechanism includes a guiding plate slidably mounted on the load-bearing platform along the radial direction of the stage; a fixing plate fixedly mounted on the load-bearing platform is arranged on the side of the guiding plate away from the stage, and a second compression spring is arranged between the guiding plate and the fixing plate; a blocking rod is arranged on the side of the guiding plate close to the stage, and the blocking rod prevents the guiding plate from moving toward the direction close to the stage, and the blocking rod is vertically slidably mounted on the load-bearing platform, and the blocking rod is fixedly connected to the stage.

[0009] As a further solution of the present invention, the guide plate is arc-shaped, and covers the circumference of the culture dish after the guide plate is corrected.

[0010] As a further solution of the present invention, the guide plate is covered with leather.

[0011] As a further solution of the present invention, a heating strip is provided on the inner wall of the sliding groove, and the heating strip is used to control the temperature of the culture dish.

[0012] As a further solution of the present invention, the light source is a strip-shaped light tube, and the strip-shaped light tube is arranged at intervals along the circumference of the support column.

[0013] As a further solution of the present invention, the brightness of the strip light tube is adjustable.

[0014] As a further solution of the present invention, the support columns are arranged at the center inside the incubator. Ventilation openings are provided on the box wall of the incubator, and filter screens are arranged at the ventilation openings.

[0015] The present invention also provides an intelligent cultivation method for plant pathogenic bacteria, and its main steps are as follows:

[0016] S1: Prepare the culture medium required for cultivating plant pathogenic bacteria and place the culture medium in a petri dish.

[0017] S2: Inoculate the well-separated plant pathogenic bacteria into the petri dish in S1.

[0018] S3: Place the petri dish in S2 on the stage in the incubator and adjust the required light intensity and temperature.

[0019] S4: Take out the petri dish after culturing for 2 - 3 days.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] 1. The present invention can not only automatically block the circumferential side wall of the petri dish to prevent external light from entering the petri dish through the circumferential side wall of the petri dish, resulting in the phototaxis of plant bacteria to adhere to the wall; but also reflect the external light to the center of the petri dish through a reflector, enabling the plant bacteria to reproduce in the middle of the petri dish, which is convenient for observation. In addition, the present invention can also automatically adjust the reflection angle of the reflector according to the liquid level height in the petri dish, so that the reflector can always reflect the light to the center of the liquid surface in the petri dish, facilitating subsequent observation and improving the efficiency of the experiment.

[0022] 2. The present invention can automatically adjust the reflection angle of the reflector according to the liquid level height in the petri dish. The present invention judges the height of the petri dish placed on the stage by the position height of the stage, and drives the deflection angle of the reflector through the downward movement distance of the stage, so that the reflector can be automatically adjusted as the height of the petri dish changes, enabling the light reflected by the reflector to always follow the petri dish, and enabling the cultivated plant pathogenic bacteria to reproduce in large quantities at the center of the petri dish, which is convenient for subsequent observation by the staff.

[0023] 3. The implementation method provided in this embodiment is that three centering plates perform radial movement for centering, and three-point centering makes the positioning more accurate. The present invention determines whether the culture dish is placed on the loading platform by whether the loading platform moves downward, and uses the downward movement of the loading platform to trigger the centering plate to perform centering. The structure is simple, easy to use, accurately positioned, improving the efficiency of the experiment and the accuracy of observation. The present invention sets the shape of the centering plate to be an arc that more conforms to the circumferential surface of the culture dish. This setting makes the movement of the culture dish more stable during centering on the one hand, avoiding the culture dish from shaking during movement, causing the culture medium in the culture dish to escape, resulting in unnecessary waste and saving resources; on the other hand, the arc-shaped centering plate is covered with leather to further shade the circumferential side wall of the culture dish, preventing light from entering the culture dish from this place, improving the shading of the circumferential side wall of the culture dish, ensuring that bacteria will not adhere to the wall due to phototaxis, making subsequent observation extremely inconvenient and affecting the efficiency of the experiment and the accuracy of observation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0025] Figure 1 Structural schematic diagram of an intelligent plant pathogenic bacteria culture system of the present invention;

[0026] Figure 2 Structural schematic diagram of the loading platform, culture dish and light regulating mechanism of the present invention;

[0027] Figure 3 For the present invention Figure 2 Structural schematic diagram of removing the culture dish;

[0028] Figure 4 For the present invention Figure 3 Partial enlarged schematic diagram of part A in the present invention;

[0029] Figure 5 Cross-sectional view of the light regulating mechanism of the present invention;

[0030] Figure 6 Structural schematic diagram of the light regulating mechanism and centering mechanism of the present invention;

[0031] Figure 7 Structural schematic diagram of the sliding groove of the present invention;

[0032] Figure 8 Cross-sectional view of the light regulating mechanism and centering mechanism of the present invention;

[0033] Figure 9 For the present inventionFigure 8 Partial enlarged view of part B

[0034] Figure 10 Process flow diagram of an intelligent plant pathogenic bacteria culture method of the present invention

[0035] In the attached drawings, the list of components represented by each reference numeral is as follows

[0036] 11 - support column, 12 - carrier platform, 13 - light source, 14 - stage, 15 - sliding groove, 16 - first compression spring, 21 - reflector, 22 - support rod, 23 - rack, 24 - first rotating shaft, 25 - second rotating shaft, 26 - gear, 27 - first synchronous pulley, 28 - second synchronous pulley, 31 - guiding plate, 32 - fixing plate, 33 - second compression spring, 34 - blocking rod, 41 - heating strip, 42 - culture dish, 51 - incubator, 52 - ventilation opening, 53 - filter screen Detailed implementation manner

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the attached drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work belong to the scope of protection of the present invention

[0038] Please refer to Figure 1-10 , an intelligent plant pathogenic bacteria culture system, including a support column 11 fixedly arranged on a frame, a light source 13 is arranged on the support column 11; a carrier platform 12 is rotatably installed at intervals along the axial direction of the support column 11, a stage 14 for placing a culture dish 42 is arranged on the carrier platform 12, a sliding groove 15 is vertically opened on the carrier platform 12, the stage 14 is vertically slidably installed in the sliding groove 15, and a first compression spring 16 is arranged between the stage 14 and the bottom of the sliding groove 15; a light regulating mechanism is arranged beside the stage 14, the light regulating mechanism includes a reflector 21, and the reflector 21 is used to reflect the light emitted by the light source 13 to the center of the liquid surface of the culture dish 42; the light regulating mechanism adjusts the reflection angle of the reflector 21 according to the displacement of the stage 14

[0039] During the actual culture process of certain plant bacteria with phototaxis, because the light source 13 is outside the culture dish 42, the plant bacteria will adhere to the wall due to phototaxis, making subsequent observations extremely inconvenient and affecting the efficiency of the experiment and the accuracy of the observation. Therefore, as Figure 3 , Figure 8As shown, the present invention places the culture dish 42 inoculated with plant pathogenic bacteria on the loading platform 14 on the support platform 12, and the loading platform 14 is driven by the gravity of the culture dish 42 to move vertically downward in the sliding groove 15 to squeeze the first compression spring 16. Because the culture dish 42 is placed on the loading platform 14, the culture dish 42 will move downward with the loading platform 14 and sink into the sliding groove 15, so that the circumferential side wall of the culture dish 42 is blocked by the side wall of the sliding groove 15, and the external light source 13 cannot enter the culture dish 42 through the circumferential side wall of the culture dish 42, so as to avoid the plant bacteria from adhering to the wall towards light, making the subsequent observation of the experimenter extremely inconvenient, affecting the efficiency of the experiment and the accuracy of the observation. After the circumferential side wall of the culture dish 42 is blocked, in order to facilitate the experimenter to observe the cultivation status of the plant pathogenic bacteria in the culture dish 42, it is best to allow the plant pathogenic bacteria to multiply in large quantities in the center of the culture dish, so as to provide favorable observation conditions for the subsequent observation of the experimenter. Therefore, the dimming mechanism of the present invention can also reflect the light emitted by the light source 13 on the support column 11 to the center of the culture dish 42, so that the cultivated plant pathogenic bacteria can multiply in large quantities in the center of the culture dish 42, which is convenient for subsequent observation by staff.

[0040] As a further solution of the present invention, the dimming mechanism includes a support rod 22 fixedly installed next to the stage 14, a rack 23 is fixedly installed at the bottom edge of the stage 14, the rack 23 is located next to the support rod 22, a first rotating shaft 24 is rotatably installed at the lower end of the support rod 22, and a second rotating shaft 25 is rotatably installed at the upper end; a gear 26 and a first synchronous wheel 27 are fixedly installed on the first rotating shaft 24, and the gear 26 is meshed with the rack 23; a second synchronous wheel 28 is fixedly installed on the second rotating shaft 25, and the first synchronous wheel 27 is synchronously driven with the second synchronous wheel 28; the reflector 21 is fixedly installed on the second rotating shaft 25.

[0041] like Figure 2As shown in the figure, the present invention reflects the light source 13 emitted from the support column 11 to the liquid surface of the culture dish 42 through the reflector 21 disposed beside the stage 14, so that the cultivated phytopathogenic bacteria can multiply in large numbers at the center of the culture dish 42. However, in the above actual cultivation process, since the culture dish 42 moves with the stage 14 in the sliding groove 15, when cultivating different phytopathogenic bacteria and using different culture media, the weight of the culture dish 42 for each cultivation may be different, the distance that the culture dish 42 moves downward with the stage 14 may also be different, and the liquid surface of the culture medium in the culture dish 42 will also change. At this time, if a fixed reflection angle is set, the light reflected by the reflector 21 cannot always be guaranteed to be reflected exactly at the center of the culture medium in the culture dish 42, which will affect the subsequent observation by the staff. Therefore, the light regulating mechanism provided by the present invention can also automatically adjust the reflection angle of the reflector 21 according to the liquid level height in the culture dish 42, so that the reflector 21 can always reflect the light to the center of the liquid surface of the culture dish 42, facilitating subsequent observation and improving the efficiency of the experiment. As Figure 8 、 Figure 9 shown, when placing the culture dish 42 inoculated with phytopathogenic bacteria on the stage 14, the stage 14 drives the culture dish 42 to slide vertically downward in the sliding groove 15 due to gravity. As Figure 9 shown, since the downward movement of the stage 14 will cause the rack 23 fixedly arranged on the stage 14 to move downward, the rack 23 drives the adjacent gear 26 to rotate counterclockwise, and the gear 26 will drive the first rotating shaft 24 to rotate counterclockwise. As Figure 8 shown, when the first rotating shaft 24 rotates counterclockwise, through the synchronous transmission of the first synchronous pulley 27 and the second synchronous pulley 28, the second rotating shaft 25 will also rotate counterclockwise. At this time, the reflector 21 fixedly installed on the second rotating shaft 25 will be adjusted in angle as the second rotating shaft 25 rotates, increasing the reflection angle, so that the light reflected by the reflector 21 can deflect downward as the stage 14 moves downward, always following the center of the culture dish 42 placed on the stage 14. The present invention can automatically adjust the reflection angle of the reflector 21 according to the liquid level height in the culture dish 42. The present invention judges the height of the culture dish 42 placed on the stage 14 through the position height of the stage 14, and drives the reflector 21 to deflect the angle through the downward movement distance of the stage 14, so that the reflector 21 can be automatically adjusted as the height of the culture dish 42 changes, so that the reflected light can always follow the culture dish 42, enabling the cultivated phytopathogenic bacteria to multiply in large numbers at the center of the culture dish 42, facilitating the subsequent observation by the staff.

[0042] As Figure 6As shown in the figure, as a further solution of the present invention, the rearview mirror 21 is a convex mirror. The convex mirror has the function of converging light. The purpose of this setting is to reflect more light, ensure the light intensity of the petri dish 42, and provide a strong guarantee for the cultivation of phytopathogenic bacteria. In addition, light can generate heat. The setting of the rearview mirror 21 in the present invention is beneficial to preventing the loss of heat in the cultivation environment and is beneficial to heat preservation.

[0043] As a further solution of the present invention, a guiding mechanism is circumferentially arranged on the stage 14; the guiding mechanism includes a guiding plate 31 slidably installed on the bearing platform 12 along the radial direction of the stage 14; on the side of the guiding plate 31 away from the stage 14, there is a fixing plate 32 fixedly installed on the bearing platform 12. A second compression spring 33 is arranged between the guiding plate 31 and the fixing plate 32; on the side of the guiding plate 31 close to the stage 14, there is a blocking rod 34. The blocking rod 34 prevents the guiding plate 31 from moving towards the stage 14. The blocking rod 34 is vertically slidably installed on the bearing platform 12, and the blocking rod 34 is fixedly connected to the stage 14.

[0044] In the actual use process of the present invention, the bottom surface size of petri dishes 42 of different specifications may not be exactly the same as the support surface size of the stage 14. Therefore, the position of the petri dish 42 placed on the stage 14 is not fixed. In order to ensure that the light reflected by the rearview mirror 21 can move along with the petri dish 42 and always be reflected at the central position of the petri dish 42, at this time, the petri dish 42 needs to be positioned to prevent the rearview mirror 21 from reflecting on the side wall of the petri dish 42, causing the cultivated bacteria to adhere to the wall, resulting in inconvenient subsequent observation and affecting the efficiency of the experiment and the accuracy of observation. As Figure 4 shown, the present invention also sets a guiding mechanism to guide and position the petri dish 42 placed on the stage 14, so that the petri dish 42 can be positioned at the center of the stage 14. As Figure 4 、 Figure 8 shown, when working, the stage 14 moves downward due to gravity. At this time, the stage 14 will drive the blocking rod 34 to move downward. The blocking rod 34 will cancel the block on the guiding plate 31. The guiding plate 31 moves towards the stage 14 under the drive of the second compression spring 33. The guiding plate 31 contacts the petri dish 42 placed on the stage 14 and is then guided to the center of the stage 14. The implementation method provided in this embodiment is that three guiding plates 31 perform radial movement guiding, and three-point centering makes the positioning more accurate. The present invention judges whether the petri dish 42 is placed on the stage 14 by whether the stage 14 moves downward, and uses the downward movement of the stage 14 to trigger the guiding plate 31 to perform guiding. The structure is simple, easy to use, accurately positioned, and improves the efficiency of the experiment and the accuracy of observation.

[0045] As a further solution of the present invention, the guiding plate 31 is arc-shaped, and after guiding, it covers the circumferential surface of the culture dish 42. As Figure 8 shown, the purpose of this setting is to make the shape of the guiding plate 31 more conform to the circumferential surface shape of the culture dish 42, making the movement of the culture dish 42 more stable during guiding, avoiding the culture dish 42 from shaking during movement, causing the culture medium in the culture dish 42 to escape, resulting in unnecessary waste and saving resources.

[0046] As a further solution of the present invention, the guiding plate 31 is treated with leather covering. The purpose of this setting is to further shade the circumferential side wall of the culture dish 42, prevent light from entering the culture dish 42 from this place, improve the shading of the circumferential side wall of the culture dish 42, and ensure that bacteria do not adhere to the wall due to phototaxis, making subsequent observations extremely inconvenient and affecting the efficiency of the experiment and the accuracy of the observation.

[0047] As a further solution of the present invention, a heating strip 41 is provided on the inner side wall of the sliding groove 15, and the heating strip 41 is used to control the temperature of the culture dish 42. As Figure 7 shown, during the actual cultivation process of plant pathogenic bacteria, due to the requirements of plant pathogenic bacteria for the environmental temperature, it is necessary to heat the culture dish 42 to ensure an appropriate temperature. In the present invention, a heating strip 41 is provided on the inner side wall of the sliding groove 15. When the culture dish 42 slides and falls into the sliding groove 15 along with the loading platform 14, the heating strip 41 provided on the inner side wall of the sliding groove 15 just heats the culture dish 42, and the heating distance is short. In addition, due to the shape design of the sliding groove 15, only the upper outlet can effectively avoid heat dissipation, which is beneficial to controlling the temperature of the culture dish 42, with a simple structure and energy saving.

[0048] As a further solution of the present invention, the light source 13 is a strip-shaped lamp tube, and the strip-shaped lamp tubes are arranged at intervals along the circumference of the support column 11. As Figure 2 shown, the purpose of this setting is to be able to cultivate multiple groups of culture dishes 42 in this way, improving the cultivation efficiency.

[0049] As a further solution of the present invention, the brightness of the strip-shaped lamp tube is adjustable. The purpose of this setting is to be able to set multiple experimental groups with different light intensities in an incubator 51, observe the influence of the variable of light intensity on cultivation, and facilitate the experiment.

[0050] As a further solution of the present invention, the support column 11 is arranged at the center inside the incubator 51, ventilation openings 52 are opened on the box wall of the incubator 51, and a filter screen 53 is arranged at the ventilation openings 52. As Figure 1As shown, the purpose of this setting is to facilitate the ventilation of the incubator 51. The purpose of setting the filter screen 53 is to prevent external dust from entering the incubator 51 through the ventilation port 52, contaminating the culture medium in the culture dish 42 and affecting the cultivation of plant pathogenic bacteria.

[0051] The present invention also provides an intelligent method for cultivating plant pathogenic bacteria, and its main steps are as follows:

[0052] S1: Prepare the culture medium required for cultivating plant pathogenic bacteria and place the culture medium in the culture dish 42;

[0053] S2: Inoculate the well-isolated plant pathogenic bacteria into the culture dish 42 in S1;

[0054] S3: Place the culture dish 42 in S2 on the stage 14 in the incubator 51 and adjust the required light intensity and temperature;

[0055] S4: Take out the culture dish 42 after culturing for 2 - 3 days.

Claims

1. An intelligent plant pathogenic bacteria culture system, characterized in that: The invention comprises a support column (11) fixedly mounted on a frame, wherein a light source (13) is arranged on the support column (11); a bearing platform (12) is rotatably mounted along the axial direction of the support column (11); a loading platform (14) for placing a culture dish (42) is arranged on the loading platform (12); a sliding groove (15) is vertically opened on the loading platform (12); the loading platform (14) is vertically slidably mounted on the sliding groove (15); a first compression spring (16) is arranged between the loading platform (14) and the bottom of the sliding groove (15); a dimming mechanism is arranged on the side of the loading platform (14); the dimming mechanism comprises a reflector (21); the reflector (21) is used to reflect light emitted by the light source (13) to the center of the liquid surface of the paper culture dish (42); the dimming mechanism adjusts the reflection angle of the reflector (21) according to the displacement of the loading platform (14); The dimming mechanism comprises a support rod (22) fixedly arranged beside the loading platform (14); a rack (23) is fixedly arranged at the bottom edge of the loading platform (14); the rack (23) is located beside the support rod (22); a first rotating shaft (24) is rotatably mounted on the lower end of the support rod (22); and a second rotating shaft (25) is rotatably mounted on the upper end; a gear (26) and a first synchronous wheel (27) are fixedly mounted on the first rotating shaft (24); the gear (26) is meshed with the rack (23); a second synchronous wheel (28) is fixedly mounted on the second rotating shaft (25); the first synchronous wheel (27) and the second synchronous wheel (28) are synchronously connected in transmission; and the reflector (21) is fixedly mounted on the second rotating shaft (25).

2. The intelligent plant pathogenic bacteria culture system according to claim 1, wherein: The reflector (21) is a convex mirror.

3. The intelligent plant pathogenic bacteria culture system according to claim 1, wherein: A guiding mechanism is arranged in the circumferential direction of the sample carrier (14); the guiding mechanism comprises a guiding plate (31) slidably mounted on the carrier (12) along the radial direction of the sample carrier (14); a fixing plate (32) fixedly mounted on the carrier (12) is arranged on the side of the guiding plate (31) away from the sample carrier (14), and a second compression spring (33) is arranged between the guiding plate (31) and the fixing plate (32); a blocking rod (34) is arranged on the side of the guiding plate (31) close to the sample carrier (14), and the blocking rod (34) prevents the guiding plate (31) from moving in a direction close to the sample carrier (14), and the blocking rod (34) is vertically slidably mounted on the carrier (12), and the blocking rod (34) is fixedly connected to the sample carrier (14).

4. An intelligent plant pathogenic bacteria culture system according to claim 3, characterized in that: The guiding plate (31) is arc-shaped, and after guiding, the guiding plate (31) covers the circumference of the culture dish (42).

5. An intelligent plant pathogenic bacteria culture system according to claim 4, characterized in that: The guide plate (31) is covered with leather.

6. The intelligent plant pathogenic bacteria culture system according to claim 1, wherein: A heating strip (41) is provided on the inner side wall of the sliding groove (15), and the heating strip (41) is used to control the temperature of the culture dish (42).

7. An intelligent plant pathogenic bacteria culture system according to claim 1, characterized in that: The light source (13) is a strip-shaped light tube, and the strip-shaped light tubes are arranged at intervals along the circumference of the support column (11).

8. An intelligent plant pathogenic bacteria culture system according to claim 7, characterized in that: The brightness of the strip light tube is adjustable.

9. The intelligent plant pathogenic bacteria culture system according to claim 1, wherein: The support column (11) is arranged at the center of the incubator (51); a vent (52) is provided on the wall of the incubator (51); and a filter (53) is provided at the vent (52).

10. An intelligent culture method for phytopathogenic bacteria, applicable to the culture system described in any one of the above claims 1 to 9, characterized in that: The main steps are as follows: S1: Configure the culture medium required for culturing phytopathogenic bacteria, and place the culture medium in a petri dish (42); S2: Inoculate the well-separated phytopathogenic bacteria into the petri dish (42) in S1; S3: Place the petri dish (42) in S2 on the stage (14) in the incubator (51), and adjust the required light intensity and temperature; S4: Take out the petri dish (42) after culturing for 2 - 3 days.

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