Reaction cartridge and detection device

Abstract

The present application discloses a reaction cartridge and a detection device, wherein the reaction cartridge comprises: a cartridge body, on which a driving slot and a plurality of reagent slots are provided; a connector, comprising a connecting plate, which covers the first end of the cartridge body, on which a first sample loading hole and a plurality of vents are provided, wherein the first sample loading hole is connected to a reagent slot, and the plurality of vents are respectively connected to a plurality of reagent slots; a cover member, which is arranged on the connector, on which a second sample loading hole is provided, and the second sample loading hole is used to be connected to the first sample loading hole during sample loading; one of the connector and the cover member is provided with a balancing gas slot, which is selectively connected or not connected to a plurality of vents; an adapter, which is slidably arranged on the second end of the cartridge body, and the adapter connects the driving slot with different reagent slots in a time-sharing manner; and a piston member, which is sealingly and slidably installed in the driving slot. The detection device comprises the above-mentioned reaction cartridge. The solution provided by the present application can improve efficiency and avoid environmental pollution.

Description

Technical Field

[0001] The present application belongs to the field of biological detection technology, and in particular, relates to a reaction cartridge and a detection device. Background Art

[0002] Nucleic acid testing is widely used in various fields of biomedicine, especially in the detection of infectious disease pathogens. The results of nucleic acid testing are an important criterion for diagnosis. The traditional nucleic acid testing process is cumbersome, including steps such as nucleic acid extraction, PCR amplification and result detection. In each of the above steps, different detection reagents need to be manually added to the reaction device in sequence, which places high demands on the detection operators and the experimental environment. Improper manual operation is prone to experimental errors, and the detection process is carried out in an open environment, which is easy to pollute the environment. Summary of the invention

[0003] The embodiments of the present application provide a reaction cartridge and a detection device, which can reduce experimental errors and avoid environmental pollution during the detection process.

[0004] In one aspect, an embodiment of the present application provides a reaction cartridge, comprising:

[0005] A cartridge body, wherein a driving slot and a plurality of reagent slots are provided on the cartridge body, a first through hole is provided at the bottom of the driving slot, and a plurality of second through holes are provided at the bottoms of the plurality of reagent slots;

[0006] The connecting member comprises a connecting plate, which is arranged at the first end of the cartridge body and covers the reagent tank, and is provided with a first sample loading hole and a plurality of vent holes, wherein the first sample loading hole is connected to one reagent tank, and the plurality of vent holes are respectively connected to the plurality of reagent tanks;

[0007] A cover member is arranged on the connecting member, and a second sample adding hole is arranged on the cover member, and the second sample adding hole is used to communicate with the first sample adding hole when adding a sample;

[0008] A balancing gas groove is formed in one of the connecting member and the covering member, and the balancing gas groove can be selectively connected to or not connected with the plurality of vent holes;

[0009] An adapter is slidably disposed at the second end of the card box body, and an adapter groove is disposed on the adapter. One end of the adapter groove is connected to the first through hole, and the other end of the adapter groove can be connected to one of the plurality of second through holes as the adapter slides;

[0010] The piston member penetrates the cover member and the connecting member and is sealed and slidably installed in the driving groove.

[0011] In some embodiments, a first connecting hole is opened on the connecting plate, a second connecting hole is opened on the covering member, the first connecting hole, the second connecting hole and the driving groove are connected in sequence, and the piston member passes through the first connecting hole and the second connecting hole and is slidably installed in the driving groove.

[0012] In some embodiments, the cartridge body is cylindrical, the drive slot is disposed at the center of the cartridge body, and a plurality of reagent slots are disposed in a ring shape surrounding the drive slot.

[0013] In some embodiments, one end of the connecting disk is sealed and fixed to the card box body, and the other end of the connecting disk is slidably connected to the cover member. The cover member is in the shape of a circular disk concentric with the card box body. The cover member can rotate around its axis and slide relative to the connecting disk. The second sample loading hole can be selectively connected or disconnected with the first sample loading hole as the cover member rotates.

[0014] In some embodiments, the balancing gas groove is disposed on the cover member and formed on a surface of a side where the cover member and the connecting member are in contact with each other, and the balancing gas groove is recessed toward a side away from the connecting member.

[0015] In some embodiments, the cover member can be switched between a closed state and an open state by rotating, and the balance gas groove can be connected or disconnected with the vent hole as the cover member rotates;

[0016] In the open state, the second sample adding hole is connected to the first sample adding hole, and the balance gas groove is not connected to the vent hole;

[0017] In the covered state, the second sample adding hole is not connected to the first sample adding hole, and the balance gas groove is connected to the ventilation hole.

[0018] In some embodiments, a first actuating structure is disposed on the covering member, and the first actuating structure is used to drive the covering member to rotate.

[0019] In some embodiments, the reaction cartridge further comprises a first fixing member, the first fixing member is fixed to the first end of the cartridge body, and the connecting member and the covering member are clamped between the cartridge body and the first fixing member.

[0020] In some embodiments, the connecting member also includes a connecting ring, which is connected to the periphery of the connecting disk, and the two ends of the connecting ring are respectively sealed and fixed to the connecting disk and the covering member, the balancing gas groove is formed in the connecting member and is composed of the space enclosed by the connecting disk and the connecting ring, and a sealing member is installed in the second sample loading hole.

[0021] In some embodiments, the reaction card box also includes an actuating assembly, which includes an actuating member and a cover member. The cover member is disposed in the balancing air groove and covers all the air holes. One end of the actuating member is connected to the cover member, and the other end of the actuating member passes through the connecting disk and the card box body to extend out of the card box body to operate the actuating member. The actuating member can move back and forth toward the cover member to make the cover member cover or open the air holes. When the cover member covers the air holes, the balancing air groove is not connected to the air holes, and when the cover member opens the air holes, the balancing air groove is connected to the air holes.

[0022] In some embodiments, a second actuating structure is disposed at one end of the adapter away from the card box body, and the second actuating structure is used to drive the adapter to rotate.

[0023] In some embodiments, the reaction cartridge further comprises a second fixing member, the second fixing member is fixed to the second end of the cartridge body, and the adapter is rotatably mounted between the cartridge body and the second fixing member.

[0024] In some embodiments, a rotating assembly is disposed between the adapter and the second fixing member.

[0025] On the other hand, the present application provides a detection device, which includes a reaction cartridge as described in any of the above schemes.

[0026] Beneficial effects of this application:

[0027] The reaction cartridge provided in the embodiment of the present application is provided with a drive slot and a plurality of reagent slots on its cartridge body. A plurality of reagents can be pre-buried in the plurality of reagent slots. The drive slot can be connected to different reagent slots respectively by rotating the adapter. When performing nucleic acid detection, it is only necessary to add the sample to a specific reagent slot. After that, the reaction reagent in the previous reagent slot can be injected into the next reagent slot through the drive slot in sequence according to the operation sequence, so as to realize automatic nucleic acid extraction, PCR amplification, and result detection and other operations. No manual intervention is required throughout the detection process, eliminating experimental errors caused by manual operation. At the same time, the reaction cartridge is sealed to avoid environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solution of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0029] Figure 1 is a schematic diagram of the structure of a reaction cartridge provided in some embodiments of the present application;

[0030] Figure 2 is a schematic diagram of an exploded structure of a reaction cartridge provided in some embodiments of the present application;

[0031] Figure 3 is a right side view of a cartridge body of a reaction cartridge provided in some embodiments of the present application;

[0032] Figure 4 is a schematic diagram of the structure of the connector of the reaction cartridge provided in some embodiments of the present application;

[0033] Figure 5 The structure of the cover of the reaction cartridge provided in some embodiments of the present application is schematically shown. Figure 1 ;

[0034] Figure 6 The structure of the cover of the reaction cartridge provided in some embodiments of the present application is schematically shown. Figure 2 ;

[0035] Figure 7 is a schematic structural diagram of a first fixing member of a reaction cartridge provided in some embodiments of the present application;

[0036] Figure 8 is a schematic structural diagram of an adapter of a reaction cartridge provided in some embodiments of the present application;

[0037] Fig. 9 is a schematic structural diagram of a second fixing member of a reaction cartridge provided in some embodiments of the present application;

[0038] Fig.10 is a schematic structural diagram of a rotary assembly of a reaction cartridge provided in some embodiments of the present application;

[0039] Fig.11 is a schematic structural diagram of a piston member of a reaction cartridge provided in some embodiments of the present application;

[0040] Fig.12 is a schematic diagram of an exploded structure of a reaction cartridge provided in other embodiments of the present application;

[0041] Fig.13 is a schematic structural diagram of a connector of a reaction cartridge provided in other embodiments of the present application;

[0042] Fig.14 The structure of the cover of the reaction cartridge provided in other embodiments of the present application is schematically shown. Figure 1 ;

[0043] Fig.15 The structure of the cover of the reaction cartridge provided in other embodiments of the present application is schematically shown. Figure 2 ;

[0044] Fig.16 It is a schematic diagram of the structure of the actuating assembly of the reaction cartridge provided in other embodiments of the present application.

[0045] The following are marked in the figure:

[0046] 1. Cartridge body; 11. Main body; 12. Connecting part; 13. Driving slot; 14. First through hole; 15. Reagent slot; 16. Second through hole; 2. Connecting piece; 21. Connecting plate; 211. First connecting hole; 212. First sample adding hole; 213. Ventilation hole; 22. Connecting ring; 23. Connecting hole wall; 24. Sample adding hole wall; 3. Covering piece; 31. Second connecting hole; 32. Second sample adding hole; 33. First actuating structure; 34. Positioning part; 35. Ball groove; 36. Ball; 37. Sealing piece; 4. Balancing gas groove; 5. First fixing member; 51. Annular slide rail; 52. Limiting wall; 53. Limiting groove; 54. Operating hole; 6. Actuating assembly; 61. Actuating member; 62. Cover member; 7. Adapter member; 71. Adapter body; 711. Adapter groove; 72. Adapter connecting portion; 721. Second actuating structure; 8. Second fixing member; 81. Bottom wall portion; 82. Side wall portion; 83. Accommodating groove; 84. Third through hole; 9. Rotating assembly; 91. Rotating bearing; 92. Gasket; 10. Piston member; 101. Piston portion; 102. Piston rod portion. DETAILED DESCRIPTION

[0047] The features and exemplary embodiments of various aspects of the present application will be described in detail below. In order to make the purpose, technical solutions and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only intended to explain the present application, rather than to limit the present application. For those skilled in the art, the present application can be implemented without the need for some of these specific details. The following description of the embodiments is only to provide a better understanding of the present application by illustrating the examples of the present application.

[0048] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "include..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.

[0049] The directional terms in the description of this application are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as limiting this application.

[0050] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The embodiments will be described in detail below in conjunction with the accompanying drawings.

[0051] On the one hand, the present application provides a reaction cartridge, which can be used in nucleic acid detection and other biological detection fields. Figure 1-Figure 3 As shown, the reaction cartridge provided in the embodiment of the present application includes: a cartridge body 1, a connecting member 2, a covering member 3, a balancing gas groove 4, an adapter member 7 and a piston member 10. A driving groove 13 and a plurality of reagent grooves 15 are provided on the cartridge body 1. A first through hole 14 is provided at the bottom of the driving groove 13, and a second through hole 16 is provided at the bottom of the reagent groove 15.

[0052] The connecting member 2 includes a connecting plate 21, which is arranged at the first end of the cartridge body 1 and covers the reagent tank 15. The connecting plate 21 is provided with a first sample loading hole 212 and a plurality of vents 213. The first sample loading hole 212 is connected to one reagent tank 15, and the plurality of vents 213 are respectively connected to the plurality of reagent tanks 15. The covering member 3 is arranged on the connecting member 2, and the covering member 3 is provided with a second sample loading hole 32, which is used to be connected to the first sample loading hole 212 when the sample is added. The balancing gas groove 4 is formed in one of the connecting member 2 and the covering member 3, and the balancing gas groove 4 can be selectively connected to or not connected to the plurality of vents 213. The adapter 7 is slidably arranged at the second end of the cartridge body 1, and the adapter 7 is provided with an adapter groove 711, one end of which is connected to the first through hole 14, and the other end of which can be connected to one of the plurality of second through holes 16 as the adapter 7 slides. The piston member 10 penetrates the cover member 3 and the connecting member 2 and is sealed and slidably installed in the driving groove 13 .

[0053] The reaction cartridge provided in the embodiment of the present application is provided with a driving slot 13 and a plurality of reagent slots 15 on the cartridge body 1. A plurality of reagents can be pre-buried in the plurality of reagent slots 15, and the driving slot 13 can be connected to different reagent slots 15 respectively by the rotation of the adapter 7. When performing nucleic acid detection, it is only necessary to add the sample to the initial reagent slot 15, and then the reaction reagent in the previous reagent slot 15 can be injected into the next reagent slot 15 through the driving slot 13 in sequence according to the operation sequence, so as to realize automatic nucleic acid extraction, PCR amplification and result detection and other operations, without manual intervention in the whole process, eliminating the experimental error caused by manual operation, and the reaction cartridge is sealed to avoid pollution to the environment.

[0054] like Figure 2 and Figure 3 As shown, in some embodiments, the cartridge body 1 is cylindrical, the drive groove 13 is arranged at the center of the cartridge body 1, and a plurality of reagent grooves 15 are arranged in an annular shape around the drive groove 13. In some embodiments, the cartridge body 1 is in a stepped cylindrical shape, comprising a main body 11 and a connecting portion 12 both of which are cylindrical, and the radius of the connecting portion 12 is greater than the radius of the main body 11. The drive groove 13 and the reagent groove 15 pass through the connecting portion 12 and extend into the main body 11. The connecting portion 12 is arranged at the first end of the main body 11 and protrudes outward from the outer circumference of the main body 11, and the connecting portion 12 is used to connect an external fixing component.

[0055] In some embodiments, the drive slot 13 and the reagent slot 15 are both open toward the first end of the cartridge body 1, the first through hole 14 at the bottom of the drive slot 13 is connected to the end face of the second end of the cartridge body 1, and the second through hole 16 at the bottom of the reagent slot 15 is connected to the end face of the second end of the cartridge body 1. The cross-sectional projection area of ​​the first through hole 14 is smaller than the cross-sectional projection area of ​​the drive slot 13, and the cross-sectional projection area of ​​the second through hole 16 is smaller than the cross-sectional projection area of ​​the reagent slot 15. In some embodiments, the first through hole 14 and the second through hole 16 are both circular holes.

[0056] In certain embodiments, the driving groove 13 is a circular groove extending longitudinally, and the reagent groove 15 is a fan-shaped groove extending longitudinally, and the reagent groove 15 is fan-shaped and spreads out in a direction away from the driving groove 13. A plurality of reagent grooves 15 are arranged in annular equal intervals around the driving groove 13, and the number of the reagent grooves 15 can be determined according to the detection process, and the embodiment of the present application does not limit this. Of course, in other embodiments, the shape of the driving groove 13 can also be a triangle, a rectangle or other shapes, and the shape of the reagent groove 15 can also be a polygon or other suitable shapes, as long as it satisfies that a plurality of reagent grooves 15 are arranged in annular equal intervals around the driving groove 13.

[0057] The structure of the connecting member 2 is as follows Figure 4 As shown, in some embodiments, the connecting member 2 includes a connecting disk 21. A first connecting hole 211 is formed on the connecting disk 21, a second connecting hole 31 is formed on the cover member 3, the first connecting hole 211, the second connecting hole 31 and the driving groove 13 are connected in sequence, and the piston member 10 passes through the first connecting hole 211 and the second connecting hole 31 and is slidably installed in the driving groove 13.

[0058] The connecting disk 21 is used to cover multiple reagent tanks 15 and connect the covering member 3 at the same time. In some embodiments, the connecting disk 21 is in the shape of a disk, and the connecting disk 21 is concentric with the cartridge body 1, one end of the connecting disk 21 is sealed and fixed with the cartridge body 1, and the other end of the connecting disk 21 is slidably connected with the covering member 3. The first connecting hole 211 on the connecting disk 21 is arranged at the center of the connecting disk 21 and is concentric with the driving groove 13. The first sample loading hole 212 is directly connected to one of the multiple reagent tanks 15, and the projection area of ​​the first sample loading hole 212 on the cartridge body 1 is located within the enclosure of the reagent tank 15 connected thereto. The vents 213 are connected to the multiple reagent tanks 15 on the cartridge body 1 one by one, and the projection area of ​​each vent 213 on the cartridge body 1 is located within the enclosure of the reagent tank 15 connected thereto.

[0059] In some embodiments, the connection plate 21 is sealed and fixed to the end surface of the first end of the card box body 1 by gluing, ultrasonic welding or laser welding.

[0060] The cover 3 is used for adding samples and covering the connector 2 and the cartridge body 1 during the reaction process. Figure 5 and Figure 6 As shown, in some embodiments, the cover member 3 is slidably connected to the connecting disk 21, and the cover member 3 is in the shape of a circular disk concentric with the card box body 1 and the connecting member 2. The cover member 3 can rotate around its axis and slide relative to the end surface of the connecting disk 21, and the second sample loading hole 32 can be selectively connected or disconnected with the first sample loading hole 211 as the cover member 3 rotates.

[0061] The second connection hole 31 is disposed at the center of the cover 3, and the first connection hole 211, the second connection hole 31 and the driving slot 13 have the same cross-sectional shape. In some embodiments, the first connection hole 211, the second connection hole 32 and the driving slot 13 are concentric and are all circular holes.

[0062] The cover 3 has a closed state and an open state. In the closed state, the second sample loading hole 32 faces the unperforated area on the connection plate 21, and the cover 3 closes the first sample loading hole 211. When the cover 3 changes from the closed state to the open state, the cover 3 rotates around its axis and slides relative to the connection member 2 until the second sample loading hole 32 faces the first sample loading hole 211, and the sample is added into the initial reagent tank 15 through the second sample loading hole 32 and the first sample loading hole 212, that is, the sample loading operation is completed, and the cover 3 is rotated back to the closed state.

[0063] In some embodiments, a sealing layer is provided between the cover 3 and the connector 2, and the sealing layer may include sealing silicone grease, high temperature lubricating oil or other sealing materials. The sealing layer is used to improve the airtightness between the cover 3 and the connector 2 to prevent the reagent tank 15 from communicating with the outside during the test process.

[0064] like Figure 5 As shown, in some embodiments, the balancing gas groove 4 is provided on the cover 3 and formed on a side surface where the cover 3 and the connector 2 are in contact, the balancing gas groove 4 is recessed toward the side away from the connector 2, and the balancing gas groove 4 does not overlap with the area of ​​the second sample loading hole 32. The balancing gas groove 4 includes a connecting groove portion 41 in the middle and a plurality of extending groove portions 42 extending outward from the connecting groove portion 41, and the balancing gas groove 4 can be selectively connected or disconnected with the plurality of vents 213 as the cover 3 rotates.

[0065] When the cover member 3 is in the cover state, the plurality of extension grooves 42 are connected to the plurality of vent holes 213 in a one-to-one correspondence. When the cover member 3 is in the open state, the plurality of extension grooves 42 are not connected to the plurality of vent holes 213. When the balance gas groove 4 is connected to the plurality of vent holes 213, the air pressure in the plurality of reagent tanks 15 can be kept balanced, thereby preventing negative pressure from occurring in the reagent tank 15 when the reaction reagent is transferred between the reagent tank 13 and the driving tank 15, thereby ensuring the detection effect.

[0066] like Figure 6 As shown, in some embodiments, a first actuating structure 33 is provided on the side of the cover 3 facing away from the cartridge body 1, and the first actuating structure 33 is used to drive the cover 3 to rotate. The first actuating structure 33 may be a rotating handle protruding from the surface of the side of the cover 3 facing away from the cartridge body 1, and the detection operator rotates the cover 3 by rotating the rotating handle. In other embodiments, the first actuating structure 33 may also be a claw-like structure provided on the outer peripheral surface of the cover 3, and the detection operator rotates the cover 3 by rotating the claw-like structure.

[0067] like Figure 7 As shown, in some embodiments, the reaction cartridge further includes a first fixing member 5 , which is fixed to a first end of the cartridge body 1 , and the connecting member 2 and the covering member 3 are clamped between the cartridge body 1 and the first fixing member 5 .

[0068] In some embodiments, the first fixing member 5 is fixed to the connecting portion 12 of the card box body 1, and the first fixing member 5 and the connecting portion 12 of the card box body 1 are fixed together by threaded connection, bolt connection or clamping. Figure 2 In the embodiment shown, the first fixing member 5 is fixed to the cartridge body 1 by bolts. The cover member 3 is rotatably mounted between the connecting member 2 and the first fixing member 5, and the two side surfaces of the cover member 3 are slidably connected to the connecting member 2 and the first fixing member 5 respectively. Figure 1 and Figure 2 The first fixing member 5 shown in the figure is connected to the connecting portion 12 of the card box body 1 by three bolts, and those skilled in the art can determine the number of bolts as needed. In addition, in addition to bolt connection, the first fixing member 5 can also be connected to the connecting portion 12 of the card box body 1 by snap-fit, gluing or ultrasonic welding.

[0069] like Figure 1 , Figure 6 and Figure 7 As shown, in some embodiments, a plurality of ball grooves 35 arranged in an annular shape are provided on the surface of the cover member 3 facing the first fixing member 5, and balls 36 are installed in the ball grooves 35. An annular slide rail 51 is provided on the surface of the first fixing member 5 facing the cover member 3, and the annular slide rail 51 is arranged opposite to the plurality of ball grooves 35. The balls 36 are clamped in the ball grooves 35 and the annular slide rail 51 and can roll along the annular slide rail 51. By providing structures such as the balls 36 and the annular slide rail 51, the smoothness of the cover member 3 when sliding relative to the first fixing member 5 can be increased, thereby improving the convenience of operation.

[0070] In some embodiments, a limiting portion 34 is disposed on one of the cover member 3 and the first fixing member 5, and an arc-shaped limiting groove 53 is disposed on the other. The limiting portion 34 is slidably installed in the limiting groove 53, and the angle of rotation of the cover member 3 around its axis is equal to the arc of the limiting groove 53. The arc of the limiting groove 53 is the angle of the central angle formed by the two ends of the limiting groove 53 and the axis of the cartridge body 1. Figure 6 In the embodiment shown, the limiting portion 34 is a table-like structure provided on the covering member 3 and protruding toward the first fixing member 5. Figure 7 In the illustrated embodiment, a limiting wall 52 is protruded on a surface of the first fixing member 5 facing the covering member 3 , and the limiting wall 52 surrounds a limiting groove 53 . The limiting portion 34 is embedded in the limiting groove 53 and can slide along the limiting groove 53 .

[0071] In some embodiments, an operation hole 54 is provided in the middle of the first fixing member 5, and the second connection hole 31 and the rotating handle on the cover member 3 are located in the enclosed area of ​​the operation hole 54. The operation hole 54 can facilitate the detection of the operator pulling and pulling the piston member 10 and screwing the cover member 3 by rotating the handle.

[0072] The structure of the adapter 7 is as follows: Figure 8 As shown, the adapter 7 is used to connect different reagent tanks 15 with the drive tank 13. The adapter 7 includes a connected adapter body 71 and an adapter connection part 72, and the adapter body 71 and the adapter connection part 72 are both cylindrical, and the radius of the adapter body 71 is larger than the radius of the adapter connection part 72. The adapter body 71 abuts against the cartridge body 1, and the adapter groove 711 is provided on one side surface of the adapter body 71 facing the cartridge body 1. A second actuating structure 721 is provided on the side of the adapter connection part 72 facing away from the adapter body 71, and the second actuating structure 721 is used to drive the adapter 7 to rotate. Figure 8 In the illustrated embodiment, the second actuating structure 721 is a straight groove, and the detection operator applies torque to the second actuating structure 721 to drive the adapter 7 to rotate.

[0073] like Fig. 9 As shown, in some embodiments, the reaction cartridge further comprises a second fixing member 8, the second fixing member 8 is fixed to the second end of the cartridge body 1, and the adapter 7 is rotatably mounted between the cartridge body 1 and the second fixing member 8. The second fixing member 8 is fixed to the cartridge body 1 by threaded connection, bolt connection or clamping.

[0074] The second fixing member 8 includes a bottom wall portion 81 and a side wall portion 82, and the bottom wall portion 81 and the side wall portion 82 are surrounded to form a receiving groove 83, and the adapter body portion 71 is received in the receiving groove 83. In one embodiment, one end of the adapter body portion 71 abuts against the inner surface of the bottom wall portion 81, and the other end of the adapter body portion 71 abuts against the end surface of the second end of the cartridge body 1. A third through hole 84 is formed on the bottom wall portion 81, and the adapter connection portion 72 passes through the third through hole 84 and extends out of the second fixing member 8.

[0075] like Figure 2 and 10 As shown, in some embodiments, a slewing assembly 9 is provided between the adapter 7 and the second fixing member 8. The slewing assembly 9 is provided in the receiving groove 83, and the slewing assembly 9 includes a slewing bearing 91 and a gasket 92. The inner ring of the slewing bearing 91 is interference fit with the outer surface of the adapter connecting portion 72, and the outer ring of the slewing bearing 91 is interference fit with the inner surface of the side wall portion 82. The gasket 92 is provided between the slewing bearing 91 and the adapter main body portion 71, and the gasket 92 is an elastic gasket, which is used to push the adapter 7 toward the second end of the cartridge body 1.

[0076] The piston member 10 is sealed and slidably installed in the driving groove 13, and is used to adjust the volume of the driving groove 13 to realize the extraction and injection of the reagent in a certain reagent tank 15. The structure of the piston member 10 is as follows Figure 2 and Fig.11 As shown, in some embodiments, the piston member 10 includes a piston portion 101 and a piston rod portion 102, the piston portion 101 is slidably mounted in the driving groove 13 and can slide axially along the axis of the driving groove 13, the piston rod portion 102 passes through the first connecting hole 211, the second connecting hole 31 and the operating hole 54 and extends out of the first fixing member 5, and the piston rod portion 102 is used to drive the piston portion 101 to slide. In the initial state of the experiment, the piston portion 101 abuts against the bottom surface of the driving groove 13.

[0077] When the reagent in one reagent tank 15 needs to be extracted into the driving tank 13, the adapter 7 is rotated so that the two ends of the adapter tank 711 are connected to the driving tank 13 and the reagent tank 15 respectively, and the piston member 10 is slid in the direction away from the adapter 7. At this time, the volume of the driving tank 13 increases, the air pressure in the driving tank 13 decreases, and the reagent is extracted from the reagent tank 15 into the driving tank 13 via the adapter tank 711. When the reagent in the driving tank 13 needs to be injected into another reagent tank 15, the adapter 7 is rotated so that the two ends of the adapter tank 711 are connected to the driving tank 13 and the other reagent tank 15 respectively, and the piston member 10 is slid in the direction close to the adapter 7. At this time, the volume of the driving tank 13 decreases, the air pressure in the driving tank 13 increases, and the reagent is pressed from the driving tank 13 into the other reagent tank 15 via the adapter tank 711.

[0078] In some embodiments, a lubricating layer is provided between the side of the piston portion 101 and the inner surface of the driving groove 13. The lubricating layer is used to improve the sliding smoothness of the piston member 10 in the driving groove 13 and prevent the piston member 10 and the inner wall of the driving groove 13 from being worn. At the same time, the lubricating layer acts as an oil seal, which can ensure the air tightness of the driving groove 13.

[0079] The method of using the reaction cartridge provided in this embodiment is as follows: operate the first actuating structure 33 to drive the cover member 3 to slide to open the cover member 3, so that the second sample loading hole 32 is connected to the first sample loading hole 212, define the reagent tank 15 directly opposite to the first sample loading hole 212 as the initial reagent tank, and add the test sample into the initial reagent tank; slide the cover member 3 to cover the cover member 3 so that the second sample loading hole 32 is not connected to the first sample loading hole 212 and the initial reagent tank, and multiple reagent tanks 15 are connected to the balance gas tank 4 through multiple ventilation holes 213; after the sample to be tested reacts sufficiently in the initial reagent tank, rotate the adapter 7 so that the two ends of the adapter tank 711 are connected to the driving tank 13 and the initial reagent tank respectively; turn away from the adapter 7 Slide the piston member 10 in the direction to form a negative pressure environment in the driving groove 13, and draw the reagent in the initial reagent groove into the driving groove 13; rotate the adapter 7 to make the two ends of the adapter groove 711 connected with the driving groove 13 and the other reagent groove 15 respectively, slide the piston member 10 in the direction close to the adapter 7, and inject the reagent in the driving groove 13 into the other reagent groove 15; according to the operating sequence of nucleic acid detection, the reagent in the previous reagent groove 15 is injected into the next reagent groove 15 through the driving groove 13 in sequence until the test operation is completed; inject the final reaction reagent into the initial reagent groove 15 through the driving groove 13, rotate the cover 3 to the open state, and extract the final reaction reagent from the second sample loading hole 32 to realize library output.

[0080] like Fig.12As shown, in other embodiments, the present application provides a reaction cartridge of another structure. The structure of the reaction cartridge provided in this embodiment is basically the same as that of the reaction cartridge described in the above embodiments, except that the reaction cartridge provided in this embodiment does not include the first fixing member 5, and the structure of the connecting member 2 in the reaction cartridge is different from that of the connecting member 2 provided in the above embodiments. In this embodiment, the cover 3 is fixedly connected to the cartridge body 1 through the connecting member 2, and unlike the previous embodiment in which the balancing gas groove 4 is formed on the cover 3, the balancing gas groove 4 is formed on the connecting member 2 in this embodiment. Now, only the structures in this embodiment that are different from those in the above embodiments are described, and the structures in this embodiment that are the same as those in the above embodiments are not described in detail.

[0081] like Fig.13 As shown, in this embodiment, the connecting member 2 further includes a connecting ring 22, which is disposed between the connecting disk 21 and the cover member 3. The connecting ring 22 is a circular ring structure, and the connecting ring 22 extends from the periphery of the connecting disk 21 toward the cover member 3, and the two end surfaces of the connecting ring 22 are respectively sealed and fixed to the connecting disk 21 and the cover member 3. The balancing gas groove 4 is composed of a space enclosed by the connecting disk 21 and the connecting ring 22, and the balancing gas groove 4 is connected to a plurality of vents 213 on the connecting disk 21.

[0082] In some embodiments, the connecting ring 22 is integrally formed with the connecting disk 21, and the connecting ring 22 is sealed and fixed to the cover 3 by gluing, ultrasonic welding or laser welding. Alternatively, the connecting disk 21 and the connecting ring 22 can be a split structure, the connecting ring 22 is made of materials such as rubber, resin or silicone grease, and the two end surfaces of the connecting ring 22 are sealed and fixed to the connecting disk 21 and the cover 3 respectively by hot melting.

[0083] In this embodiment, the cover member 3 is directly fixed to the connecting member 2 , and the card box body 1 does not need to be provided with the first fixing member 5 to fix the cover member 3 , so the card box body 1 does not include the connecting portion 12 for connecting with the first fixing member 5 .

[0084] A connection hole wall 23 extending toward the cover 3 is provided on the connection disk 21 around the first connection hole 211, and a sample loading hole wall 24 extending toward the cover 3 is provided on the connection disk 21 around the first sample loading hole 212. Both the connection hole wall 23 and the sample loading hole wall 24 abut against the cover 3. The connection hole wall 23 and the sample loading hole wall 24 are used to isolate the balance gas groove 4 from the first connection hole 211 and the first sample loading hole 212 to ensure the normal progress of the detection process.

[0085] like Fig.14 and Fig.15As shown, in some embodiments, both side end surfaces of the cover 3 are planes, and only the second connecting hole 31 and the second sample loading hole 32 are provided on the cover 3. A sealing member 37 is installed in the second sample loading hole 32, and the second sample loading hole 32 is blocked by the sealing member 37. Since the reaction cartridge is closed by blocking the second sample loading hole 32 with the sealing member 37 before use, in this embodiment, it is not necessary to provide an actuating structure on the cover 3 for rotating the cover 3 to the closed state.

[0086] like Fig.12 and Fig.16 As shown, the reaction cartridge also includes an actuating assembly 6, which includes an actuating member 61 and a cover member 62. The cover member 62 is disposed in the balancing gas tank 4 and covers all the vents 213 before the reaction cartridge is used. One end of the actuating member 61 is connected to the cover member 62, and the other end of the actuating member 61 passes through the connecting plate 21, the cartridge body 1, and the second fixing member 8 in sequence and extends out of the second fixing member 8 to operate the cover member 62. The actuating member 61 can reciprocate toward the covering member 3 to allow the cover member 62 to cover or open a plurality of vents 213. Fig.12 In the illustrated embodiment, the connection disk 21, the cartridge body 1, and the second fixing member 8 are provided with aligned through holes for the actuator 61 to pass through, the through hole on the cartridge body 1 is formed between the bottom of a reagent tank 15 and the second end of the cartridge body 1, and the actuator 61 sequentially passes through the connection disk 21, the multiple aligned through holes provided on the cartridge body 1, and the second fixing member 8, and a reagent tank 15 on the cartridge body 1, and extends out of the second fixing member 8. The actuator 61 can be operated by the detection operator to control the opening and closing of the multiple vents 213.

[0087] When the cover plate 62 covers the vent holes 213 , the balancing gas groove 4 is not connected to all the vent holes 213 . When the cover plate 62 opens the vent holes 213 , the balancing gas groove 4 is connected to all the vent holes 213 .

[0088] The method for using the reaction cartridge provided in this embodiment is as follows: open the sealing member 37 to connect the second sample loading hole 32 with the first sample loading hole 212, define the reagent tank 15 directly opposite to the first sample loading hole 212 as the initial reagent tank, and add the test sample into the initial reagent tank; install the sealing member 37 so that the cover member 3 covers the initial reagent tank; push the actuating member 61 toward the cover member 3, and the actuating member 61 pushes the cover plate member 62 to open the multiple vents 213, so that the multiple reagent tanks 15 are connected to the balance gas tank 4 through the multiple vents 213; after the sample to be tested reacts sufficiently in the initial reagent tank, rotate the adapter 7 so that the two ends of the adapter tank 711 are connected to the driving tank 13 and the initial reagent tank respectively; Slide the piston member 10 in the direction away from the adapter 7 to form a negative pressure environment in the drive groove 13, and draw the reagent in the initial reagent groove into the drive groove 13; rotate the adapter 7 to make the two ends of the adapter groove 711 connected to the drive groove 13 and the other reagent groove 15 respectively, and slide the piston member 10 in the direction close to the adapter 7 to inject the reagent in the drive groove 13 into the other reagent groove 15; according to the operating sequence of nucleic acid detection, the reagent in the previous reagent groove 15 is injected into the next reagent groove 15 through the drive groove 13 in sequence until the test operation is completed; inject the final reaction reagent into the initial reagent groove through the drive groove 13, and extract the final reaction reagent from the second sample loading hole 32 to realize library output.

[0089] The reaction cartridge provided in the embodiment of the present application can realize sample input and library output in just one step, with low manual workload and high detection efficiency; the reaction process of the scheme is carried out in a closed reaction cartridge, one cartridge is for one person, there is no cross contamination, and pollution to the environment is avoided; the reaction cartridge of the scheme is made of injection-molded plastic material, is a disposable consumable, is easy to use, and can be used after simple training of the detection operator. The reaction cartridge has built-in reagents, and the user does not need to prepare additional reagents, and the cost is low.

[0090] In a second aspect, an embodiment of the present application provides a detection device, which includes a reaction cartridge as described in any of the above schemes. The detection device is easy to use and has high detection efficiency.

[0091] The reaction cartridge and detection device provided in the present application can be widely used in the following fields: genome library construction; as a reaction container carrying reagents for biochemical, immune and molecular detection; as a reaction container carrying reagents for pesticide residues, heavy metals and antibiotic detection; as a reaction container carrying reagents for poultry and livestock disease detection.

[0092] The above is only a specific implementation of the present application. Those skilled in the art can clearly understand that for the convenience and simplicity of description, the specific working processes of the systems, modules and units described above can refer to the corresponding processes in the aforementioned method embodiments, and will not be repeated here. It should be understood that the protection scope of the present application is not limited to this. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in this application, and these modifications or replacements should be included in the protection scope of this application.

Claims

1. A reaction cartridge, characterized in that: include: A cartridge body (1), wherein the cartridge body (1) is provided with a drive slot (13) and a plurality of reagent slots (15), a first through hole (14) is provided at the bottom of the drive slot (13), and a plurality of second through holes (16) are respectively provided at the bottom of the plurality of reagent slots (15); A connecting member (2), comprising a connecting plate (21), the connecting plate (21) being arranged at a first end of the cartridge body (1) and covering the reagent tank (15), the connecting plate (21) being provided with a first sample loading hole (212) and a plurality of vent holes (213), the first sample loading hole (212) being in communication with one of the reagent tanks (15), and the plurality of vent holes (213) being in communication with a plurality of the reagent tanks (15) respectively; A cover member (3) is arranged on the connecting member (2), and a second sample adding hole (32) is arranged on the cover member (3), and the second sample adding hole (32) is used to communicate with the first sample adding hole (212) when adding a sample; a balancing air groove (4) formed in one of the connecting member (2) and the covering member (3), the balancing air groove (4) being capable of selectively being connected to or not connected to the plurality of vent holes (213); an adapter (7) slidably disposed at the second end of the card box body (1), the adapter (7) being provided with an adapter groove (711), one end of the adapter groove (711) being connected to the first through hole (14), and the other end of the adapter groove (711) being able to be connected to one of the plurality of second through holes (16) as the adapter (7) slides; A piston member (10) passes through the cover member (3) and the connecting member (2) and is sealingly slidably mounted in the driving groove (13); One end of the connection disk (21) is sealed and fixed to the cartridge body (1), and the other end of the connection disk (21) is slidably connected to the cover member (3). The cover member (3) is in the shape of a circular disk concentric with the cartridge body (1). The cover member (3) can rotate around its axis and slide relative to the connection disk (21). The second sample loading hole (32) can be selectively connected to or disconnected from the first sample loading hole (212) as the cover member (3) rotates.

2. The reaction cartridge according to claim 1, characterized in that: The connecting plate (21) is provided with a first connecting hole (211), the covering member (3) is provided with a second connecting hole (31), the first connecting hole (211), the second connecting hole (31) and the driving groove (13) are sequentially connected, and the piston member (10) passes through the first connecting hole (211) and the second connecting hole (31) and is slidably mounted in the driving groove (13).

3. The reaction cartridge according to claim 1, characterized in that: The cartridge body (1) is cylindrical, the driving groove (13) is arranged at the center of the cartridge body (1), and a plurality of reagent grooves (15) are arranged in a ring shape around the driving groove (13).

4. The reaction cartridge according to claim 3, characterized in that: The balancing air groove (4) is arranged on the cover member (3) and is formed on a surface of a side where the cover member (3) and the connecting member (2) are in contact with each other, and the balancing air groove (4) is recessed toward a side away from the connecting member (2).

5. The reaction cartridge according to claim 4, characterized in that: The covering member (3) can be switched between a covering state and an opening state by rotating, and the balancing air groove (4) can be connected to or disconnected from the vent hole (213) as the covering member (3) rotates; In the open state, the second sample adding hole (32) is connected to the first sample adding hole (212), and the balancing gas groove (4) is not connected to the ventilation hole (213); In the covered state, the second sample loading hole (32) is not connected to the first sample loading hole (212), and the balance gas groove (4) is connected to the ventilation hole (213).

6. The reaction cartridge according to claim 2, characterized in that: The covering member (3) is provided with a first actuating structure (33), and the first actuating structure (33) is used to drive the covering member (3) to rotate.

7. The reaction cartridge according to claim 2, characterized in that: The reaction cartridge further comprises a first fixing member (5), wherein the first fixing member (5) is fixed to a first end of the cartridge body (1), and the connecting member (2) and the covering member (3) are clamped between the cartridge body (1) and the first fixing member (5).

8. The reaction cartridge according to claim 1, characterized in that: The connecting member (2) further comprises a connecting ring (22), the connecting ring (22) being connected to the periphery of the connecting disk (21), the two ends of the connecting ring (22) being sealed and fixed to the connecting disk (21) and the covering member (3) respectively, the balancing gas groove (4) being formed in the connecting member (2) and being constituted by a space enclosed by the connecting disk (21) and the connecting ring (22), and a sealing member (37) being installed in the second sample loading hole (32).

9. The reaction cartridge according to claim 8, characterized in that: The reaction cartridge further comprises an actuating assembly (6), the actuating assembly (6) comprising an actuating member (61) and a cover member (62), the cover member (62) being arranged in the balancing gas groove (4) and used to cover all the vent holes (213), one end of the actuating member (61) being connected to the cover member (62), and the other end of the actuating member (61) passing through the connecting plate (21) and the cartridge body (1) so as to be able to be operatively contacted from the outside of the cartridge body (1) The actuating member (61) is operated, and the actuating member (61) can reciprocate toward the covering member (3) so that the cover member (62) covers or opens the vent hole (213); when the cover member (62) covers the vent hole (213), the balancing gas groove (4) is not connected to the vent hole (213); when the cover member (62) opens the vent hole (213), the balancing gas groove (4) is connected to the vent hole (213).

10. The reaction cartridge according to claim 1, characterized in that: A second actuating structure (721) is provided at one end of the adapter (7) facing away from the card box body (1), and the second actuating structure (721) is used to drive the adapter (7) to rotate.

11. The reaction cartridge according to claim 1, characterized in that: The reaction cartridge further comprises a second fixing member (8), wherein the second fixing member (8) is fixed to the second end of the cartridge body (1), and the adapter (7) is rotatably mounted between the cartridge body (1) and the second fixing member (8).

12. The reaction cartridge according to claim 11, characterized in that: A rotating assembly (9) is provided between the adapter (7) and the second fixing member (8).

13. A detection device, characterized in that: Comprising a reaction cartridge as described in any one of claims 1-12.

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