A fluorescence detection optical path system and spectroscopic structure

Abstract

The present invention discloses a fluorescence detection optical path system and a spectroscopic structure, wherein the fluorescence detection optical path system includes a light source, a collimating lens, a light source filter, a prism lens group, a dichroic mirror, and a fluorescence camera. The collimating lens is arranged in the post-stage optical path of the light source; the light source filter is arranged in the post-stage optical path of the collimating lens; the prism lens group is arranged in the post-stage optical path of the light source filter; the dichroic mirror is arranged in the post-stage optical path of the prism lens group; and the fluorescence camera is arranged above the sample reagent. The spectroscopic structure of the present invention includes a plurality of prism lens groups. The fluorescence detection optical path system of the present invention can improve the detection efficiency of the sample reagent, and accordingly, the spectroscopic structure can divide the excitation light into a plurality of light beams with equal light intensity, so as to meet the detection requirements of large-throughput sample reagents.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a fluorescence detection optical path system and a spectroscopic structure. Background Art

[0002] In traditional fluorescence detection devices, usually one detection head corresponds to one sample reagent, and then all sample reagents are detected by scanning. The detection principle is that during the fluorescence detection process, the excitation light emitted by the light source, after being collimated and filtered, is directed to the sample reagent through a dichroic mirror, thereby stimulating the sample reagent to produce fluorescence, and the fluorescence signal is captured by the fluorescence camera, thereby realizing the detection of the sample reagent.

[0003] In the current environment, with the growth of the global population, the demand for testing is also increasing, and traditional fluorescence detection devices (such as fluorescence quantitative PCR) can only detect one sample reagent at a time, which will cause a decrease in detection efficiency. Therefore, traditional fluorescence detection devices have a slow detection speed for large-throughput sample reagents. Summary of the invention

[0004] In order to solve the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a fluorescence detection optical path system and a spectroscopic structure. The fluorescence detection optical path system can improve the detection efficiency of sample reagents. Accordingly, the spectroscopic structure can divide the excitation light into multiple beams of equal intensity to meet the detection requirements of high-throughput sample reagents.

[0005] The technical solution adopted by the present invention to solve the technical problem is: a fluorescence detection optical path system, comprising:

[0006] light source;

[0007] A collimating lens, which is arranged in the rear optical path of the light source to collimate the light beam emitted by the light source;

[0008] A light source filter, which is arranged in the rear optical path of the collimating lens and filters the collimated light beam to form an excitation light;

[0009] A prism sheet group, wherein the prism sheet group is arranged in the post-stage optical path of the light source filter, and splits the excitation light so that the excitation light is divided into a plurality of light beams with equal light intensity through the prism sheet group;

[0010] A dichroic mirror, which is arranged in the rear optical path of the prism lens assembly so that the split excitation light is directed toward the sample reagent;

[0011] A fluorescence camera, wherein the fluorescence camera is disposed above the sample reagent so that the fluorescence generated by the sample reagent when stimulated by the excitation light is photographed by the fluorescence camera;

[0012] The prism sheet group includes a plurality of prism sheets, one side surface of the prism sheet is a light receiving surface, the other side surface is a light splitting surface, and the light receiving surface of the prism sheet is perpendicular to the optical axis of the light beam.

[0013] The present invention is based on the above technical solution. When detecting sample reagents, the light beam emitted by the light source is collimated by the collimating lens and filtered by the light source filter to form an excitation light, and the excitation light is split by the prism lens group to divide the excitation light into multiple light beams with equal light intensity, and the multiple light beams can detect different sample reagents respectively, thereby meeting the detection requirements for high-throughput sample reagents and improving the detection efficiency. At the same time, the prism lens used in the present invention has a light-receiving surface and a light-splitting surface. When in use, the light-receiving surface is vertically distributed with the optical axis of the light beam, so that the light beam can be fully reflected in the prism lens, reducing the light intensity loss of the light beam.

[0014] According to the fluorescence detection optical path system of the present invention, the present invention may also have the following additional technical features.

[0015] Furthermore, according to the fluorescence detection optical path system of the present invention, the light receiving surface of the prism sheet is a plane, and the light splitting surface of the prism sheet has equilateral triangular convex strips distributed in an array. Through the arrangement of the plane and the equilateral triangular convex strips, the light beam can be divided into two light beams with equal light intensities when passing through the prism sheet.

[0016] Furthermore, according to the fluorescence detection optical path system of the present invention, a reflector is further provided in the front optical path of the dichroic mirror, and the light beam separated by the prism lens assembly is reflected onto the mirror surface of the dichroic mirror by the reflector.

[0017] Further, according to the fluorescence detection optical path system of the present invention, the prism sheet group includes three prism sheets, and the three prism sheets are arranged in the shape of an equilateral triangle;

[0018] Among the three prism sheets, the light splitting surface of one prism sheet faces the light receiving surfaces of the other two prism sheets.

[0019] Furthermore, according to the fluorescence detection optical path system of the present invention, the prism sheet group includes six prism sheets, three of which constitute a first spectroscopic component, and the other three prism sheets constitute a second spectroscopic component, and the second spectroscopic component is arranged in the post-stage optical path of the first spectroscopic component.

[0020] Furthermore, according to the fluorescence detection optical path system of the present invention, in the second spectroscopic component, the lengths of two prism sheets are consistent, and the length of the remaining prism sheet is adapted to the length of the coverage area of ​​the light beam after being split by the first spectroscopic component, so that the light beam after being split by the first spectroscopic component is completely reflected into the second spectroscopic component.

[0021] Based on the technical solution of the fluorescence detection optical path system of the present invention, the present invention also provides a spectroscopic structure, which includes:

[0022] A prism sheet group, wherein the prism sheet group is provided with at least one group;

[0023] The prism sheet group includes a plurality of prism sheets, one side surface of the prism sheet is a light receiving surface, the other side surface is a light splitting surface, and the light receiving surface of the prism sheet is perpendicular to the optical axis of the light beam.

[0024] Optionally, the prism sheet group includes three prism sheets.

[0025] Optionally, the light-receiving surface of the prism sheet is a plane, and the light-splitting surface of the prism sheet has regular triangular protrusions distributed in an array. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a schematic structural diagram of a prism sheet of the present invention;

[0027] Figure 2 is a side view of a prism sheet of the present invention;

[0028] Figure 3 is a schematic diagram of the light splitting principle of the prism sheet of the present invention;

[0029] Figure 4 is a schematic structural diagram of a prism sheet assembly according to Embodiment 1 of the present invention;

[0030] Figure 5 is a schematic structural diagram of a second light splitting component in Embodiment 2 of the present invention;

[0031] Figure 6 is a schematic diagram of an optical path system of Embodiment 1 of the present invention;

[0032] Figure 7 It is a schematic diagram of the optical path system of Example 2 of the present invention. DETAILED DESCRIPTION

[0033] The present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the relevant invention, rather than to limit the invention. It is also necessary to explain that, for ease of description, only the parts related to the invention are shown in the accompanying drawings.

[0034] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0035] In one aspect of the present invention, the present invention proposes a fluorescence detection optical path system. The fluorescence detection optical path system includes a light source, a collimating lens, a light source filter 200, a prism lens group, a dichroic mirror 300, and a fluorescence camera 400. Among them, the collimating lens is arranged in the post-stage optical path of the light source to collimate the light beam emitted by the light source; the light source filter 200 is arranged in the post-stage optical path of the collimating lens to filter the collimated light beam to form an excitation light; the prism lens group is arranged in the post-stage optical path of the light source filter to split the excitation light, so that the excitation light is divided into several beams of equal light intensity through the prism lens group; the dichroic mirror 300 is arranged in the post-stage optical path of the prism lens group so that the excitation light after splitting is directed to the sample reagent; the fluorescence camera 400 is arranged above the sample reagent so that the fluorescence generated by the sample reagent 500 when stimulated by the excitation light is photographed by the fluorescence camera 400.

[0036] Figure 1 is a schematic structural diagram of a prism sheet of the present invention; Figure 2 It is a side view of the prism sheet of the present invention.

[0037] refer to Figure 1 and Figure 2 In the fluorescence detection optical path system of the present invention, the prism sheet group includes a plurality of prism sheets 100, one side surface of the prism sheet 100 is a light receiving surface, and the other side surface is a light splitting surface. In the fluorescence detection optical path system of the present invention, the light receiving surface of the prism sheet 100 is perpendicular to the optical axis of the light beam, thereby ensuring that the incident light can vertically enter the prism sheet 100.

[0038] like Figure 2 As shown, the light receiving surface of the prism sheet 100 is a plane 101, and the light splitting surface of the prism sheet 100 has regular triangular convex strips 102 distributed in an array. The convex strips 102 are distributed along the width direction of the prism sheet 100, and the roots of each two adjacent convex strips 102 are connected together so that there is no gap between the roots of the convex strips 102.

[0039] In the fluorescence detection optical path system of the present invention, since the convex strip 102 of the prism sheet 100 is an equilateral triangle, the top angle of the convex strip 102 is 60°. In this case, when the light beam enters the prism sheet 100 from the light receiving surface, the light beam completely penetrates the plane 101 and then emits in the direction of the convex strip 102. Figure 3Since the angle between the edge of the convex strip 102 and the plane of the prism sheet 100 is 60°, the light beam is totally reflected after encountering the edge of the convex strip 102 and is reflected toward the other edge of the convex strip 102. The reflected light beam is perpendicular to the other edge of the convex strip 102, so the light beam directly passes through the other edge of the convex strip 102. Since the convex strip 102 has two edges, the light beam passing through the prism sheet 100 is divided into two light beams with equal light intensity by the convex strip 102, and the angle between the two light beams is 120°.

[0040] The prism sheet 100 of the present invention may be made of optical plastic or glass, and the glass is formed by a molding process.

[0041] In the fluorescence detection optical path system of the present invention, a reflector 600 is further provided in the front optical path of the dichroic mirror 300 , and the light beam separated by the prism lens assembly is reflected onto the mirror surface of the dichroic mirror 300 by the reflector 600 .

[0042] Based on the fluorescence detection optical path system of the present invention, the present invention further proposes a light splitting structure, which can split the light beam into multiple light beams with equal light intensities.

[0043] The light splitting structure of the present invention comprises:

[0044] A prism sheet group, wherein the prism sheet group is provided with at least one group;

[0045] The prism sheet assembly includes a plurality of prism sheets 100, one side surface of the prism sheet 100 is a light receiving surface, and the other side surface is a light splitting surface. The light receiving surface of the prism sheet 100 is perpendicular to the optical axis of the light beam. Figure 1 and Figure 2 The light receiving surface of the prism sheet 100 is a plane 101, and the light splitting surface of the prism sheet 100 has regular triangular convex strips 102 distributed in an array.

[0046] The present invention is described below with reference to specific embodiments. It should be noted that these embodiments are merely illustrative and do not limit the present invention in any way.

[0047] Example 1

[0048] In this embodiment, the excitation light is divided into four beams. Therefore, in this embodiment, the prism sheet group includes three prism sheets 100, the specifications of the three prism sheets 100 are the same, and the three prism sheets 100 are connected end to end, so that the three prism sheets 100 are arranged in the shape of an equilateral triangle. The arrangement of the three prism sheets is referred to as Figure 4 The arrangement method of .

[0049] Among the three prism sheets 100, the splitting surface of one prism sheet 100 faces the light receiving surfaces of the other two prism sheets, that is, the splitting surface of one prism sheet 100 is connected to the light receiving surfaces of the other two prism sheets 100, and the light receiving surface of the one prism sheet 100 and the splitting surfaces of the other two prism sheets 100 are both arranged outward.

[0050] According to the arrangement of the prism sheet 100 of this embodiment, Figure 6 As shown, after the excitation light penetrates the prism sheet 100 with the light receiving surface facing outward, the excitation light is divided into two light beams with an angle of 120°, and the two light beams are respectively directed to the other two prism sheets 100. After the two light beams pass through the other two prism sheets 100, they are again divided into two light beams, a total of 4 light beams, and the light intensity of these 4 light beams is consistent. Since one of the two light beams that are finally split is parallel to the excitation light, the light beam is directly directed to the dichroic mirror 300, and the other light beam has an angle of 120° with the excitation light, so it needs to be reflected by the reflector 600 to be a light beam parallel to the excitation light, and then directed to the dichroic mirror 300. Since the mirror surface of the dichroic mirror 300 is distributed at 45° with the excitation light, the 4 light beams are directed to different positions of the dichroic mirror 300, and the dichroic mirror 300 reflects the 4 light beams into 4 groups of different sample reagents for detection.

[0051] Example 2

[0052] In this embodiment, the excitation light is set to be divided into 16 light beams. Therefore, this embodiment can add another prism sheet group on the basis of embodiment 1, that is, in this embodiment, the prism sheet group used includes six prism sheets 100, three of which constitute a first light splitting component, which is also the prism sheet group in embodiment 1, and the other three prism sheets constitute a second light splitting component, which is arranged in the rear light path of the first light splitting component.

[0053] In this embodiment, in the second light splitting component, as Figure 5 As shown, the lengths of two prism sheets 100 are consistent, and the lengths of these two prism sheets 100 are also consistent with the length of the prism sheet 100 in the first spectrometer component. The length of the remaining prism sheet is adapted to the length of the coverage area of ​​the light beam after being split by the first spectrometer component, so that the light beam after being split by the first spectrometer component is completely reflected into the second spectrometer component.

[0054] like Figure 7 As shown, the four light beams split by the first light splitting component are then split by the second light splitting component respectively, so that the light beams are divided into 16 light beams with equal light intensity.

[0055] In this embodiment, the light splitting principle of the first light splitting component and the second light splitting component is consistent with the light splitting principle of the prism sheet assembly in Embodiment 1, and will not be described in detail here.

[0056] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present application is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with (but not limited to) technical features with similar functions disclosed in the present application.

[0057] Except for the technical features described in the specification, the remaining technical features are known technologies to those skilled in the art. In order to highlight the innovative features of the present invention, the remaining technical features will not be described here in detail.

Claims

1. A fluorescence detection optical path system, characterized in that: include: light source; A collimating lens, which is arranged in the rear optical path of the light source to collimate the light beam emitted by the light source; A light source filter, which is arranged in the rear optical path of the collimating lens and filters the collimated light beam to form an excitation light; A prism sheet group, wherein the prism sheet group is arranged in the post-stage optical path of the light source filter, and splits the excitation light so that the excitation light is divided into a plurality of light beams with equal light intensity through the prism sheet group; A dichroic mirror, which is arranged in the rear optical path of the prism lens assembly so that the split excitation light is directed toward the sample reagent; A fluorescence camera, wherein the fluorescence camera is disposed above the sample reagent so that the fluorescence generated by the sample reagent when stimulated by the excitation light is photographed by the fluorescence camera; The prism sheet group includes three prism sheets, which are arranged in the shape of an equilateral triangle. One side surface of the prism sheet is a light-receiving surface, and the other side surface is a light-splitting surface. The light-receiving surface of the prism sheet is perpendicular to the optical axis of the light beam, the light-receiving surface of the prism sheet is a plane, and the light-splitting surface of the prism sheet has equilateral triangular convex strips distributed in an array. Among the three prism sheets, the light splitting surface of one prism sheet faces the light receiving surfaces of the other two prism sheets.

2. The fluorescence detection optical path system according to claim 1, characterized in that: A reflector is also provided in the front-stage optical path of the dichroic mirror, and the light beam separated by the prism lens assembly is reflected onto the mirror surface of the dichroic mirror through the reflector.

3. The fluorescence detection optical path system according to claim 2, characterized in that: The prism sheet group includes six prism sheets, three of which constitute a first light splitting component, and the other three prism sheets constitute a second light splitting component, and the second light splitting component is arranged in the rear light path of the first light splitting component.

4. The fluorescence detection optical path system according to claim 3, characterized in that: In the second beam splitter component, two prism sheets have the same length, and the length of the remaining prism sheet is adapted to the length of the light beam coverage after being split by the first beam splitter component, so that the light beam after being split by the first beam splitter component is completely reflected into the second beam splitter component.

5. A light splitting structure, characterized in that: include: A prism sheet group, wherein the prism sheet group is provided with at least one group; The prism sheet group includes three prism sheets, which are arranged in the shape of an equilateral triangle. One side surface of the prism sheet is a light-receiving surface, and the other side surface is a light-splitting surface. The light-receiving surface of the prism sheet is perpendicular to the optical axis of the light beam. The light-receiving surface of the prism sheet is a plane, and the light-splitting surface of the prism sheet has equilateral triangular convex strips distributed in an array. Among the three prism sheets, the light splitting surface of one prism sheet faces the light receiving surfaces of the other two prism sheets.

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