Gene detection device

Abstract

The present invention discloses a gene detection device, which relates to the field of gene detection and includes a safety cabinet, an air flow control system, a first processing module for processing amplification reagents and PCR plates, a second processing module for processing reagents, pipette tips and gene samples, a sample addition module for adding amplification reagents into the micro-wells of the PCR plate, a purification processing module for processing gene samples, an amplification and detection module for gene amplification and detection, a heat sealer for sealing the PCR plate, a logistics transportation module A, a logistics transportation module B and a logistics transportation module C; through the gene detection device of the present invention, automated, pollution-free and high-efficiency gene detection is achieved. At the same time, during the detection process, only a small part involves manual operation, and the manual operation intensity is low.

Description

Technical Field

[0001] The present invention relates to the field of gene detection, and particularly to a gene detection device. Background Art

[0002] Gene detection refers to analyzing the base sequence of a specific DNA fragment, that is, the arrangement of adenine, thymine, cytosine, and guanine; a gene is the basic unit of heredity, a DNA or RNA sequence carrying genetic information, which transmits genetic information to the next generation through replication and guides the synthesis of proteins to express the genetic information it carries, thereby controlling the trait expression of an individual organism. Gene detection is a technology for detecting DNA through blood, other body fluids, or cells. It is to take peripheral venous blood or other tissue cells of the person to be tested, amplify their gene information, and then use a specific device to detect the DNA molecular information in the cells of the person to be tested, and analyze the gene types, gene defects, and whether their expression functions are normal contained in it, so that people can understand their own gene information, clarify the cause of the disease or predict the risk of suffering from a certain disease. Gene detection can diagnose diseases and can also be used for predicting disease risks.

[0003] The gene detection devices of the prior art cannot achieve full automation of the detection process, resulting in low gene detection efficiency.

[0004] Therefore, there is an urgent need to develop a gene detection device to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to design a gene detection device to solve the above problems.

[0006] The present invention realizes the above purpose through the following technical solutions:

[0007] A gene detection device, comprising:

[0008] A safety cabinet; the safety cabinet is divided into upper and lower relatively isolated layers, and each layer of the safety cabinet is divided into three relatively isolated installation rooms. The installation rooms on the upper layer are respectively the first installation room A, the first installation room B, and the first installation room C, and the installation rooms on the lower layer are respectively the second installation room A, the second installation room B, and the second installation room C; a first switch door assembly A is provided between the first installation room A and the second installation room A, a first switch door assembly B is provided between the first installation room B and the second installation room B, a first switch door assembly C is provided between the first installation room C and the second installation room C, a second switch door assembly A is provided between the second installation room A and the second installation room B, and a second switch door assembly B is provided between the second installation room B and the second installation room C;

[0009] An air flow control system; the air flow control system is used to control the air pressure conditions in the first installation room A, the first installation room B, and the first installation room C;

[0010] The first processing module for processing amplification reagents and PCR plates;

[0011] The second processing module for processing reagents, pipette tips and gene samples;

[0012] The sample loading module for loading amplification reagents into the micro-wells of the PCR plate;

[0013] The purification processing module for processing gene samples;

[0014] The amplification and detection module for gene amplification and detection;

[0015] The heat sealer for sealing the PCR plate;

[0016] Logistics transportation module A;

[0017] Logistics transportation module B;

[0018] Logistics transportation module C;

[0019] Among them, the sample loading module and the first processing module are installed in the first installation room A, the purification processing module, the second processing module and the heat sealer are installed in the first installation room B, the amplification and detection module is installed in the first installation room C, and the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively installed in the second installation room A, the second installation room B and the second installation room C; the logistics transportation module A, the logistics transportation module B and the logistics transportation module C are respectively located below the sample loading module, the purification processing module and the amplification and detection module; during transportation, the PCR plate containing the amplification reagent passes through the sample loading module and the PCR plate is placed at the transfer end of the logistics transportation module A through the first switch door assembly A. When the logistics transportation module A transfers the PCR plate to the second switch door assembly A, the PCR plate is placed at the transfer end of the logistics transportation module B. When the logistics transportation module B transfers the PCR plate to the first switch door assembly B, it enters the first installation room B through the purification processing module, and the purified gene sample is added to the PCR plate. After heat sealing by the heat sealer, the PCR plate containing the gene sample passes through the purification processing module and the first switch door assembly B and is placed at the transfer end of the logistics transportation module B. When the logistics transportation module B transfers the PCR plate to the second switch door assembly B, the PCR plate is placed at the transfer end of the logistics transportation module C. When the logistics transportation module C transfers the PCR plate to the first switch door assembly C, it enters the first installation room C through the purification processing module, and the amplification and detection module performs gene detection on the gene sample in the PCR plate.

[0020] The beneficial effects of the present invention are as follows: The gene detection equipment of the present invention achieves automated, pollution-free and high-efficiency gene detection. At the same time, during the detection process, only a small part involves manual operation, and the manual operation intensity is low. Description of the Drawings

[0021] Figure 1 is a three - dimensional structure schematic diagram of the present invention;

[0022] Figure 2 is a three - dimensional structure schematic diagram of the sample - adding module of the present invention;

[0023] Figure 3 is a structural schematic diagram of the second processing module of the present invention Figure 1 ;

[0024] Figure 4 is a structural schematic diagram of the second processing module of the present invention Figure 2 ;

[0025] Figure 5 is a structural schematic diagram of the heating module in the second processing module of the present invention;

[0026] Figure 6 is a structural schematic diagram of the placement rack D in the second processing module of the present invention;

[0027] Figure 7 is a structural schematic diagram of the opening - and - closing component in the second processing module of the present invention;

[0028] Figure 8 is an open - cover schematic diagram of the opening - and - closing component in the purification processing module of the present invention;

[0029] Figure 9 is a structural schematic diagram of the purification processing module of the present invention Figure 3 ;

[0030] Figure 10 is a structural schematic diagram of the second processing module of the present invention Figure 4 ;

[0031] Figure 11 is a structural schematic diagram of the purification module in the purification processing module of the present invention;

[0032] Figure 12 is a structural schematic diagram of the heat - sealing shell of the present invention;

[0033] Figure 13 is a structural schematic diagram of the air - guiding component in the second processing module of the present invention;

[0034] Figure 14 is a structural schematic diagram of the liquid - transferring part B in the purification processing module of the present invention;

[0035] Figure 15 is a usage schematic diagram of the liquid - transferring part B in the purification processing module of the present invention;

[0036] Figure 16 is a structural schematic diagram of the amplification detection module of the present invention;

[0037] Figure 17 It is a schematic structural diagram of the translation component C in the amplification detection module of the present invention;

[0038] Figure 18 It is a schematic structural diagram of the logistics transportation module A and the logistics transportation module B of the present invention;

[0039] Figure 19 It is a schematic diagram when the logistics transportation module A and the logistics transportation module B of the present invention are being transferred;

[0040] Figure 20 It is a schematic diagram before the logistics transportation module B and the logistics transportation module C of the present invention are transferred;

[0041] Figure 21 It is a schematic diagram after the logistics transportation module B and the logistics transportation module C of the present invention are transferred;

[0042] Figure 22 It is a schematic diagram of the air flow direction of the air flow control system of the present invention;

[0043] Among them, the corresponding reference numerals are:

[0044] 1 - First installation chamber A, 2 - First installation chamber B, 3 - First installation chamber C, 4 - Second installation chamber A, 5 - Second installation chamber B, 6 - Second installation chamber C, 7 - First switch door assembly A, 8 - First switch door assembly B, 9 - First switch door assembly C, 10 - Second switch door assembly A, 11 - Second switch door assembly B, 18 - High - efficiency filter A, 19 - Induced draft fan A, 22 - Bracket, 23 - Lifting component B, 24 - Translation component E, 26 - Scanner, 29 - Pipetting part B, 30 - Translation component I;

[0045] 12 - Transfer component, 1201 - X - direction moving component, 1202 - Y - direction moving component, 1203 - Z - direction moving component, 1204 - Pipetting part A, 1205 - Claw C;

[0046] 13 - First processing module, 1301 - Placing rack A, 1302 - Placing rack B, 1303 - Cooling module A;

[0047] 14 - Second processing module, 1401 - Placing rack C, 1402 - Placing rack D, 1403 - Placing rack E, 1404 - Placing rack F, 1405 - Temporary storage rack, 1406 - Heating module, 1408 - Induced draft component, 1409 - Spring piece, 1410 - Placing groove, 1411 - Transfer position, 1412 - Vibration part A;

[0048] 15 - Amplification detection module, 1501 - Optical detector, 1502 - Heating base, 1503 - Thermal cover assembly, 1504 - Translation component C;

[0049] 16 - Jaw A, 1601 - Jaw control part A;

[0050] 17 - Purification module, 170 - 1 Thermal oscillation chamber, 1702 - Magnetic attraction part, 1703 - Translation component A, 1704 - Reaction mounting rack, 1705 - Vibration part B;

[0051] 20 - Opening and closing component, 2001 - Lifting component A, 2002 - Jaw B, 2003 - Fixed block A, 2004 - Fixed block B, 2005 - Translation component B, 2006 - Snap ring, 2007 - High - friction soft pad;

[0052] 21 - Translation component D, 2101 - Motor D, 2102 - Driving wheel D, 2103 - Driven wheel D, 2104 - Transmission belt D, 2105 - Mounting plate D, 2106 - Slide rail;

[0053] 25 - Solid - liquid waste discharging part, 2501 - Translation component G, 2502 - Cover plate;

[0054] 28 - Heat - sealing shell, 2801 - Third switch - door component, 2802 - Heat - sealer, 2803 - Mounting plate F, 2804 - Translation component H;

[0055] 31 - Liquid - receiving component; 3101 - Liquid - receiving plate, 3102 - Liquid - receiving groove, 3103 - Mounting shaft, 3104 - Liquid - receiving rack, 3105 - Groove, 3106 - Boss, and 3107 - Torsion spring. Detailed implementation mode

[0056] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Usually, the components of the embodiments of the present invention described and illustrated in the accompanying drawings here can be arranged and designed in various different configurations.

[0057] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed present invention, but merely represents the selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0058] It should be noted that: Similar reference numerals and letters represent similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0059] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "left", "right", etc. are based on the orientation or positional relationships shown in the drawings, or the orientation or positional relationships in which the inventive product is customarily placed during use, or the orientation or positional relationships commonly understood by those skilled in the art. These are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation on the present invention.

[0060] In addition, the terms "first", "second", etc. are only used for distinguishing descriptions and should not be construed as indicating or implying relative importance.

[0061] In the description of the present invention, it should also be noted that unless otherwise clearly specified and limited, terms such as "set", "connected" should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] The following will describe in detail the specific embodiments of the present invention with reference to the drawings.

[0063] As Figure 1 shown, a gene detection device includes:

[0064] A safety cabinet; the safety cabinet is divided into upper and lower relatively isolated layers, and each layer of the safety cabinet is divided into three relatively isolated installation chambers. The installation chambers on the upper layer are the first installation chamber A1, the first installation chamber B2, and the first installation chamber C3 respectively, and the installation chambers on the lower layer are the second installation chamber A4, the second installation chamber B5, and the second installation chamber C6 respectively; a first switch door assembly A7 is provided between the first installation chamber A1 and the second installation chamber A4, a first switch door assembly B8 is provided between the first installation chamber B2 and the second installation chamber B5, a first switch door assembly C9 is provided between the first installation chamber C3 and the second installation chamber C6, a second switch door assembly A10 is provided between the second installation chamber A4 and the second installation chamber B5, and a second switch door assembly B11 is provided between the second installation chamber B5 and the second installation chamber C6;

[0065] An air flow control system; the air flow control system is used to control the air pressure in the first installation chamber A1, the first installation chamber B2, and the first installation chamber C3;

[0066] A first processing module 13 for processing amplification reagents and PCR plates;

[0067] The second processing module 14 for processing reagents, pipette tips, and gene samples;

[0068] The sample addition module for adding amplification reagents into the micro-wells of the PCR plate;

[0069] The purification processing module for processing gene samples;

[0070] The amplification and detection module 15 for gene amplification and detection;

[0071] The heat sealer 2802 for sealing the PCR plate;

[0072] The logistics transportation module A;

[0073] The logistics transportation module B;

[0074] The logistics transportation module C;

[0075] Among them, the sample addition module and the first processing module 13 are installed in the first installation chamber A1, the purification processing module, the second processing module 14, and the heat sealer are installed in the first installation chamber B2, the amplification and detection module 15 is installed in the first installation chamber C3, and the logistics transportation module A, the logistics transportation module B, and the logistics transportation module C are respectively installed in the second installation chamber A4, the second installation chamber B5, and the second installation chamber C6; the logistics transportation module A, the logistics transportation module B, and the logistics transportation module C are respectively located below the sample addition module, the purification processing module, and the amplification and detection module 15; during transportation, the PCR plate containing the amplification reagent passes through the first switch door assembly A7 through the sample addition module and is placed at the transfer end of the logistics transportation module A. When the logistics transportation module A transfers the PCR plate to the second switch door assembly A10, the PCR plate is placed at the transfer end of the logistics transportation module B. When the logistics transportation module B transfers the PCR plate to the first switch door assembly B8, it enters the first installation chamber B2 through the purification processing module, and the purified gene sample is added to the PCR plate. After heat sealing by the heat sealer 2802, the PCR plate containing the gene sample passes through the first switch door assembly B8 of the purification processing module and is placed at the transfer end of the logistics transportation module B. When the logistics transportation module B transfers the PCR plate to the second switch door assembly B11, the PCR plate is placed at the transfer end of the logistics transportation module C. When the logistics transportation module C transfers the PCR plate to the first switch door assembly C9, it enters the first installation chamber C3 through the purification processing module, and the amplification and detection module 15 performs gene detection on the gene sample in the PCR plate.

[0076] Such as Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 9As shown, the sample loading module, the purification processing module, and the amplification detection module all include a transfer assembly 12, a jaw A 16 for transferring the PCR plate, and a jaw control member A 1601 for controlling the opening and closing of the jaw A 16. Among them, the sample loading module and the purification processing module further include a pipetting member A 1204 for transferring liquid. Each transfer assembly 12 includes:

[0077] An X-direction moving assembly 1201 for X-direction movement; the X-direction moving assembly includes a motor A, a driving wheel A, a driven wheel A, a transmission belt A, and a mounting plate A. The mounting plate A is fixedly connected to the transmission belt A. The rotating shaft of the motor A is fixedly connected to the rotation center of the driving wheel A. The driving wheel A and the driven wheel A are driven by the transmission belt A;

[0078] A Y-direction moving assembly 1202 for Y-direction movement; the Y-direction moving assembly includes a motor B, a driving wheel B, a driven wheel B, a transmission belt B, and a mounting plate B. The mounting plate B is fixedly connected to the transmission belt B. The rotating shaft of the motor B is fixedly connected to the rotation center of the driving wheel B. The driving wheel B and the driven wheel B are driven by the transmission belt B;

[0079] A Z-direction moving assembly 1203 for Z-direction movement; the Z-direction moving assembly includes a motor C, a lead screw nut mechanism A, and a mounting plate C. The rotating shaft of the motor C is fixedly connected to the lead screw A of the lead screw nut mechanism A. The mounting plate C is fixedly connected to the nut A of the lead screw nut mechanism A. The jaw control member A 1601 and the pipetting member A 1204 are both fixedly installed on the mounting plate C;

[0080] Among them, the X-direction moving assembly 1201 of the sample loading module and the amplification detection module is installed on the mounting plate B, and the Z-direction moving assembly 1203 is fixedly installed on the mounting plate A; the Y-direction moving assembly 1202 of the purification processing module is installed on the mounting plate A, and the Z-direction moving assembly 1203 is fixedly installed on the mounting plate B.

[0081] In this embodiment, within the first installation chamber A1: The sample addition module changes the position of the pipetting member A1204 in the X, Y, and Z directions through the transfer assembly 12, so as to transfer the amplification reagent into the micro-wells of the PCR plate. Then, the clamping jaw control member A1601 controls the opening and closing of the clamping jaw A16 to grab the PCR plate, and transfers the PCR through the first switch door assembly A7 to the transfer end of the logistics transportation module A; within the first installation chamber B2: After the purification processing module purifies the gene sample, the purification processing module controls the opening and closing of the clamping jaw A16 through the clamping jaw control member A1601 to grab the PCR plate on the transfer end of the logistics transportation module B, transfers it through the first switch door assembly B8 to the first installation chamber B2, and places the purified gene sample into the micro-wells of the PCR plate through the pipetting member A1204. Then, the sealing film plate is sealed on the PCR plate containing the gene sample through the heat sealer 2802. Finally, the PCR plate containing the gene sample is placed on the transfer end of the logistics transportation module B through the clamping jaw A16 through the first switch door assembly B8; within the first installation chamber C3: The amplification and detection module 15 controls the opening and closing of the clamping jaw A16 through the clamping jaw control member A1601 to grab the PCR plate on the transfer end of the logistics transportation module C, transfers it through the first switch door assembly C9 into the first installation chamber C3, and performs amplification and optical detection on the gene sample through the amplification and detection module 15.

[0082] As Figure 2 shown, the first processing module 13 includes at least two sets of placement racks A1301, placement racks B1302, and a cooling module A1303 for cooling the amplification reagent. The test tubes containing the amplification reagent are placed on the placement rack A1301. The quantity of the amplification reagent on one set of placement rack A1301 is not less than the quantity of the amplification reagent required for one set of gene sample experiments. The PCR plates are placed on the placement rack B1302. There is an opening on the side of the first installation chamber A1. The placement rack A1301 and the placement rack B1302 can both be placed in the first installation chamber A1 in a drawer-like manner. The cooling module A1303 is installed directly below the placement rack A1301.

[0083] In this embodiment, the first processing module further includes a cleaning pool for cleaning the pipetting member A1204. After the pipetting member A1204 transfers the amplification reagent once, the transfer assembly 12 transfers the pipetting end of the pipetting member A1204 into the cleaning pool, and the cleaning pool cleans the pipetting end of the pipetting member A1204, and then performs the next amplification reagent pipetting operation. At least two PCR plates are placed on the placement rack B1302. The setting of at least two sets of placement racks A1301 ensures that the sample addition process can be carried out continuously without being affected by manual replenishment of the amplification reagent, improving the efficiency of gene detection.

[0084] As Figure 1 、 Figure 3 、 Figure 4 、Figure 5 , Figure 6 , Figure 9 , Figure 10 As shown in Figure 10 , the second processing module 14 includes at least two sets of placement racks C1401, at least two sets of placement racks D1402, a placement rack E1403, at least two sets of temporary storage racks 1405, a placement rack F1404, a vibrating member A1412 for vibrating the magnetic bead solution, a heating module 1406 for heating the lysis solution, and a cooling module B for cooling the purified gene sample. The purification reagent, the eight-well tube for the purification reaction, and the replacement pipette tip A are all placed on the placement rack C1401. The quantity of the purification reagent, the number of eight-well tubes, and the number of replacement pipette tips A on one set of the placement rack C1401 are respectively not less than the quantity of the purification reagent, the number of eight-well tubes, and the number of replacement pipette tips A required for one set of gene sample experiments. The sample tubes containing gene samples are placed on the placement rack D1402. The number of gene samples on one set of the placement rack D1402 is not less than the number of gene samples required for one set of gene sample experiments. The cleaning solution for eluting the gene sample is placed in the placement rack E1403. The replacement pipette tip B and the sealing film plate are both placed in the placement rack F1404. The PCR plate not filled with the purified gene sample is placed on the temporary storage rack 1405. The cooling module B is installed directly below the temporary storage rack 1405. An opening is provided on the side of the first installation chamber B2. The placement rack C1401, the placement rack D1402, the placement rack E1403, and the placement rack F1404 can all be placed in the first installation chamber B2 in a drawer-like manner through the opening. The second processing module further includes a transfer position 1411, and the transfer position 1411 is used to place the eight-well tubes not filled with gene samples.

[0085] In this embodiment, the heating module 1406 is used to heat the lysis solution to prevent the lysis solution from crystallizing. The height of the first end of the heating module 1406 is lower than the height of the second end of the heating module 1406. When the placement rack C1401 is inserted into the first installation chamber B2, it passes through the first end and the second end of the heating module 1406 in sequence. The vibrating member A1412 vibrates the magnetic bead solution to keep the magnetic bead solution in a uniform state and prevent its deposition from affecting the purification reaction. The setting of at least two sets of placement racks C1401, at least two sets of placement racks D1402, the placement rack E1403, at least two sets of temporary storage racks 1405, and the placement rack F1404 ensures that during manual replenishment, there are corresponding spare supplies in the first installation chamber B2 during the replenishment and replacement time to meet the usage requirements of the purification reaction process or basically have no impact on the purification reaction, so as to ensure that all processes of the purification reaction in the first installation chamber B2 can be carried out uninterruptedly, thereby achieving the purpose of improving the gene detection efficiency.

[0086] As Figure 6As shown in the figure, a plurality of placement grooves 1410 for placing sample tubes are provided on the placement rack D1402. A spring piece 1409 is provided on one side of the placement groove 1410. The direction in which the spring piece 1409 restores deformation faces the central axis of the placement groove 1410. When the sample tube is placed in the placement groove 1410, the spring piece 1409 is in pressing contact with the side wall of the sample tube.

[0087] In this embodiment, the sample tube is inserted and placed in the placement groove 1410 of the placement rack D1402. By the pressing contact between the spring piece 1409 and the side wall of the sample tube, the purpose of fixing the sample tube is achieved, avoiding the sample tube from shaking during the movement. A plurality of barcodes are provided on the placement rack. One placement groove 1410 is located between two adjacent barcodes. The placement rack C1401 is also provided with a plurality of barcodes. A test tube containing a purification reagent is located between two adjacent barcodes. The gene detection device further includes two barcode scanners 26 for scanning and identifying barcodes. The specific positions and information of each sample tube and the purification reagent are identified by the barcode scanners. An internal reference sample for comparing whether the test results are incorrect is also placed on the placement rack D1402.

[0088] The gene detection device further includes multiple sets of fixing devices, which are respectively used to fix the placement racks A1301, B1302, C1401, D1402 and E1403. Each fixing device includes a position sensor and an electromagnet. When the position sensor detects that the corresponding placement rack is inserted in place, the electromagnet adsorbs and fixes the corresponding placement rack.

[0089] In this embodiment, when the placement racks A1301 and B1302 are inserted into the first installation chamber A1, when the position sensors identify that the placement racks A1301 and B1302 are inserted in place, the electromagnet is powered on, and the electromagnet adsorbs and fixes the placement racks A1301 and B1302; when the placement racks C1401, D1402 and E1403 are inserted into the first installation chamber B2, when the position sensors identify that the placement racks C1401, D1402 and E1403 are inserted in place, the electromagnet is powered on, and the electromagnet adsorbs and fixes the placement racks C1401, D1402 and E1403.

[0090] As Figure 9 、 Figure 10 、 Figure 13 As shown in the figure, the second processing module further includes at least two sets of translation components F and at least two sets of air guiding components 1408 for sucking the aerosol generated by the gene sample. One set of translation component F is used to change the distance between the air inlet of the air guiding component 1408 and the temporary storage rack 1405. During air guiding, the air inlet of one set of air guiding component 1408 is located directly above one set of temporary storage racks 1405.

[0091] In this embodiment, the air extraction assembly includes a high-efficiency filter C and an air extraction fan D. The air outlet of the high-efficiency filter C is communicated with the air inlet of the air extraction fan D. When the PCR plate not filled with gene samples is temporarily placed on the temporary storage rack 1405, the translation assembly F moves the air inlet of the high-efficiency filter D to be directly above the PCR plate, and the air extraction fan D is started to suck the aerosol generated by the gene samples, so as to avoid the contamination of the first installation chamber B2 caused by the aerosol.

[0092] As Figure 9 , Figure 10 , Figure 11 shown, the purification processing module further includes at least one purification module 17, a high-efficiency filter A 18 and an air extraction fan A 19. Each purification module 17 includes a heating and oscillation chamber 1701, a magnetic attraction member 1702, a vibration member B 1705, a translation assembly A 1703 and a heating member. The heating and oscillation chamber 1701 is provided with a reaction mounting rack 1704, and the eight-well tubes for purification reaction are placed on the reaction mounting rack 1704. The heating action end of the heating member acts on the eight-well tubes, and the heating member is mounted on the vibration action end of the vibration member B 1705. The translation assembly A 1703 is used to move the magnetic attraction member 1702 to change the attraction force of the magnetic attraction member 1702 on the magnetic beads in the eight-well tubes. An air outlet is arranged on the side of the heating and oscillation chamber 1701. One of the air inlets of the high-efficiency filter A 18 is communicated with the air outlet of one heating and oscillation chamber 1701, and the air inlet of the air extraction fan A 19 is communicated with the air outlet of the high-efficiency filter A 18.

[0093] In this embodiment, the eight-well tubes are placed on the reaction mounting rack 1704. The heating member and the vibration member B 1705 are started to heat the gene samples in the eight-well tubes for purification processing. The vibration member B makes the gene samples and the purification reagents in the eight-well tubes mix evenly. During the purification reaction process, the air extraction fan A 19 is started to make the aerosol generated during the purification reaction process pass through the air inlet of the high-efficiency filter A 18, and the high-efficiency filter A 18 filters the aerosol. After the purification reaction, the translation assembly A 1703 moves the magnetic attraction member 1702 close to the eight-well tubes, and the magnetic attraction member 1702 adsorbs the magnetic beads in the eight-well tubes. When the magnetic beads are combined with the gene samples, the gene samples are separated from the waste liquid, and then the waste liquid is sucked out and discharged through the liquid transfer member A 1204; when the magnetic beads are not combined with the gene samples, the gene samples are separated from the magnetic beads, which is convenient for the liquid transfer member A 1204 to suck out the gene samples and transfer them into the PCR plate filled with amplification reagents.

[0094] As Figure 3 , Figure 7 , Figure 8As shown, the purification processing module further includes an opening and closing component 20 for opening and closing the tube cap in the sample tube. The opening and closing component includes a lifting component A2001, a clamping jaw B2002, a clamping jaw control part B for controlling the opening and closing of the clamping jaw B2002, and a tube body fixing part for fixing the tube body in the sample tube. The lifting action end of the lifting component A2001 acts on the clamping jaw control part B, and the tube body fixing part is arranged below the clamping jaw B2002.

[0095] In this embodiment, the sample tube is fixed by the tube body fixing part. The distance between the clamping jaws B2002 is increased by the clamping jaw control part B. The lifting component A2001 lowers the clamping jaw control part B and the clamping jaw B2002 to the position of the tube cap of the sample tube. The distance between the clamping jaws B2002 is reduced by the clamping jaw control part B, so that the clamping jaw B2002 clamps the tube cap. Then, the height of the clamping jaw control part B and the clamping jaw B2002 is raised by the lifting component A2001 to achieve the purpose of opening the cap. The reverse is the closing cap operation process.

[0096] As Figure 3 、 Figure 7 、 Figure 8 shown, the tube body fixing part includes a fixing block A2003, a fixing block B2004, and a translation component B2005 for controlling the distance between the fixing block A2003 and the fixing block B2004. The fixing block B2004 is installed directly below the clamping jaw B2002. The moving action end of the translation component B2005 acts on the fixing block A2003. The opposite side surfaces of the fixing block A2003 and the fixing block B2004 are both arc surfaces. A clamping ring 2006 for hanging the sample tube is arranged at the upper end of the arc surface of the fixing block A2003.

[0097] In this embodiment, the sample tube is taken out from the placement groove of the placement rack D1402 by the purification processing module, and then transferred into the clamping ring 2006 of the fixing block A2003. Then, the fixing block A2003 is moved by the translation component B2005, so that the side wall of the sample tube is respectively in pressing contact with the arc surfaces of the fixing block A2003 and the fixing block B2004 to achieve the purpose of fixing the sample tube. At the same time, after opening the cap and taking out the gene sample, the tube cap is closed. The translation component B2005 moves the fixing block A2003 away from the fixing block B2004. Finally, the sample tube is placed in the placement groove of the placement rack D1402 corresponding to the one when it was taken out by the purification processing module.

[0098] As Figure 7 shown, high-friction soft pads 2007 are fixedly arranged on the arc surfaces of the fixing block A2003 and the fixing block B2004.

[0099] In this embodiment, high-friction soft pads 2007 are fixedly arranged on the arc surfaces of the fixed block A 2003 and the fixed block B 2004 to enhance the squeezing force between the sample tube and the fixed block A 2003 and the fixed block B 2004 when fixing the sample tube, and enhance the fixing effect of the sample tube.

[0100] As Figure 1 , Figure 3 , Figure 4 , Figure 9 , Figure 10 As shown, the purification processing module further includes two sets of transfer components 12, a jaw C 1205 for transferring the sample tube and the octuplet tube, a jaw control part C for controlling the opening and closing of the jaw C 1205, and two sets of pipetting parts A 1204 for transferring liquid. The three pipetting parts A 1204 of the purification processing module are respectively used for transferring the purification reagent, the cleaning solution, and the gene sample. The pipetting part A 1204 for transferring the cleaning solution and the jaw control part A 1601 are installed on the mounting plate C of the same transfer component 12. The pipetting part A 1204 for transferring the gene sample and the jaw control part C are installed on the mounting plate C of the same transfer component 12. The pipetting part A 1204 for transferring the purification reagent is installed on the mounting plate C of another transfer component 12.

[0101] In this embodiment, the purification processing module altogether includes three sets of transfer components 12, a jaw C 1205 for transferring the sample tube and the octuplet tube, a jaw control part C for controlling the opening and closing of the jaw C 1205, and three sets of pipetting parts A 1204 for transferring liquid. Among them, the three pipetting parts A 1204 are respectively used for transferring the purification reagent, the cleaning solution, and the gene sample before purification. The pipetting part A 1204 for transferring the cleaning solution and the jaw control part A 1601 are installed on the mounting plate C of the same transfer component 12; the pipetting part A 1204 for transferring the gene sample before purification and the jaw control part C are installed on the mounting plate C of the same transfer component 12. The pipetting part A 1204 for transferring the purification reagent is installed on the mounting plate C of another transfer component 12, ensuring that the three transfer components 12 do not interfere with each other during the whole purification process, and at the same time, it can also ensure the simultaneous progress of multiple groups of experiments, shorten the time in the purification reaction process, and improve the detection efficiency. The model of the pipetting part A 1204 for transferring the purification reagent is ADP30110869 of TECAN; the second processing module further includes a cleaning pool. The cleaning pool is used to clean the pipetting part A 1204 for transferring the gene sample before purification. After the pipetting part A 1204 transfers an amplification reagent once, the transfer component 12 transfers the pipetting end of the pipetting part A 1204 into the cleaning pool, and the cleaning pool cleans the pipetting end of the pipetting part A 1204, and then performs the next amplification reagent pipetting operation; the replacement tip A is used to replace the pipetting tip of the pipetting part A 1204 for transferring the purification reagent, and the replacement tip B is used to replace the pipetting tip of the pipetting part A 1204 for transferring the cleaning solution.

[0102] As shown in Figure 14 and Figure 15 shown, the purification processing module further includes a pipetting member B29 for micro-pipetting, a translation assembly I30, and a liquid receiving assembly 31 for receiving the liquid from the pipetting member B29. The pipetting member B29 is used to transfer the purified gene sample. The liquid receiving assembly includes a liquid receiving plate 3101, a mounting shaft 3103, a liquid receiving rack 3104, and a rotation driving member for driving the liquid receiving rack 3104 to rotate. A liquid receiving groove 3102 is provided on the liquid receiving plate 3101. The mounting shaft 3103 and the translation assembly I30 are mounted on the same mounting plate C as the jaw control member A1601. The first end of the liquid receiving rack 3104 is rotatably mounted on the mounting shaft 3103, and the second end of the liquid receiving rack 3104 is fixedly connected to the liquid receiving plate 3101. The translation assembly I30 is used to change the relative position of the liquid receiving plate 3101 and the pipetting member B29 in the dropping direction of the pipetting needle.

[0103] In this embodiment, when performing micro-pipetting, the translation assembly I30 and the rotation driving member are started simultaneously. The pipetting member B29 moves relative to the liquid receiving plate 3101 in the dropping direction, and the rotation driving member drives the liquid receiving rack 3104 to rotate and drives the liquid receiving plate 3101 to rotate away from the pipetting member B29; when receiving liquid, the translation assembly I30 and the rotation driving member are started simultaneously. The translation assembly I30 moves relative to the liquid receiving plate 3101 in the reverse direction of the dropping direction, and the rotation driving member drives the liquid receiving rack 3104 to rotate and drives the liquid receiving plate 3101 to rotate towards the pipetting member B29. When the rotation is in place, the liquid receiving groove 3102 of the liquid receiving plate 3101 is directly below the needle of the pipetting member B29 to collect the liquid dropped by the pipetting member B29.

[0104] As shown in Figure 14 and Figure 15 shown, the rotation driving member includes a groove 3105, a boss 3106, and a torsion spring 3107. The groove 3105 is provided on the liquid receiving rack 3104, the boss 3106 is fixedly provided on the pipetting member B29, the boss 3106 is in continuous pressing contact with the liquid receiving rack 3104, the first end of the torsion spring 3107 is fixedly connected to the first end of the liquid receiving rack 3104, the second end of the torsion spring 3107 is fixedly connected to the mounting shaft 3103, and the torsion spring 3107 is always in an energy storage state.

[0105] In this embodiment, when performing micro - pipetting, the translation assembly I30 is activated, and the pipetting member B29 moves relative to the liquid - receiving plate 3101 in the dropping - liquid direction. At this time, the boss 3106 moves away from the groove 3105 of the liquid - receiving rack 3104, increasing the distance between the pipetting member B29 and the liquid - receiving rack 3104. Since the liquid - receiving rack 3104 is rotatably mounted on the mounting shaft 3103, the liquid - receiving rack 3104 drives the liquid - receiving plate 3101 to rotate in a direction away from the pipetting member B29, causing the liquid - receiving plate 3101 to move away from below the needle of the pipetting member B29. The pipetting member B29 can perform normal pipetting operations, and at the same time, the torsion spring 3107 further deforms and continues to store energy; when receiving liquid, the translation assembly I30 is activated, and the pipetting member B29 moves relative to the liquid - receiving plate 3101 in the direction opposite to the dropping - liquid direction. At this time, the boss 3106 moves closer to the groove 3105 of the liquid - receiving rack 3104, reducing the distance between the pipetting member B29 and the liquid - receiving rack 3104, and the squeezing force between the boss 3106 and the liquid - receiving rack 3104 decreases. Therefore, the torsion spring 3107 restores its deformation and releases energy, driving the liquid - receiving rack 3104 to rotate and driving the liquid - receiving plate 3101 to rotate in a direction closer to the pipetting member B29. When the rotation reaches the position, the boss 3106 is located in the groove 3105 of the liquid - receiving rack 3104, and the liquid - receiving groove 3102 of the liquid - receiving plate 3101 is located directly below the needle of the pipetting member B29 to receive and collect the liquid dropped by the pipetting member B29, preventing the liquid in the pipetting member B29 from dripping and contaminating the samples and gene detection equipment during the operation.

[0106] In some embodiments, the rotation driving member is a gear - rack. The gear is fixedly installed at the first end of the liquid - receiving rack 3104, and the rack meshes with the gear for transmission. The rack is fixedly installed on the pipetting member B29.

[0107] When performing micro - pipetting, the translation assembly I30 is activated, and the pipetting member B29 moves relative to the liquid - receiving plate 3101 in the dropping - liquid direction. At this time, the rack moves together with the pipetting member B29, and the rack meshes with the gear to drive the liquid - receiving rack 3104 and the liquid - receiving plate 3101 to rotate in a direction away from the pipetting member B29, causing the liquid - receiving plate 3101 to move away from below the needle of the pipetting member B29. The pipetting member B29 can perform normal pipetting operations; when receiving liquid, the translation assembly I30 is activated, and the pipetting member B29 moves relative to the liquid - receiving plate 3101 in the direction opposite to the dropping - liquid direction. At this time, the rack moves in the opposite direction together with the pipetting member B29, and the rack meshes with the gear to drive the liquid - receiving rack 3104 and the liquid - receiving plate 3101 to rotate in a direction closer to the pipetting member B29. When the movement and rotation reach the position, the liquid - receiving groove 3102 of the liquid - receiving plate 3101 is located directly below the needle of the pipetting member B29 to receive and collect the liquid dropped by the pipetting member B29, preventing the liquid in the pipetting member B29 from dripping and contaminating the samples and gene detection equipment during the operation.

[0108] Such as Figure 1, Figure 16 , Figure 17 As shown in Figure 16 and Figure 17 , the amplification detection module further includes at least two sets of metal bath modules, at least two sets of optical detectors 1501, and at least two sets of translation components C1504. Each metal bath module includes a thermal cover assembly 1503 and a heating base 1502. One set of thermal cover assembly 1503 is installed at the detection port of one set of optical detector 1501, and the moving end of one set of translation component C1504 acts on one heating base 1502.

[0109] In this embodiment, the transfer component 12 transfers the PCR plate on the transfer end of the logistics transportation module C to the heating base 1502. The translation component C1504 moves the heating base 1502 to move the PCR plate directly below the thermal cover assembly 1503, and then metal bath is performed to amplify the gene sample. At the same time, the optical detector 1501 performs optical detection and analysis on the amplified gene sample. After the amplification detection is completed, the translation component C1504 moves the heating base 1502 to move the PCR plate out, and then separates it from the heating base 1502 through the transfer component 12 and the gripper A, and waste disposal is carried out.

[0110] As Figure 18 , Figure 19 , Figure 20 , Figure 21 As shown in Figure 18 , Figure 19 , Figure 20 , and Figure 21 , the logistics transportation modules A, B, and C all include brackets 22 for receiving PCR plates. The logistics transportation modules A and B further include translation components D21. The translation component D21 includes a motor D2101, a slide rail 2106, a driving wheel D2102, a driven wheel D2103, a transmission belt D2104, and a mounting plate D2105. The mounting plate D2105 is fixedly connected to the transmission belt D2104. The rotating shaft of the motor D2101 is fixedly connected to the rotation center of the driving wheel D2102. The driving wheel D2102 and the driven wheel D2103 are driven by the transmission belt D2104. The mounting plate D2105 is slidably installed in the slide rail 2106, and the slide rail 2106 is fixedly installed in the installation room. Among them, the logistics transportation modules A and C include a lifting component B23 for lifting the height of the bracket 22. The lifting component B23 is fixedly installed on the mounting plate D2105. The logistics transportation module B further includes a translation component E24 for moving the bracket 22. The translation component E24 is fixedly installed on the installation D2105. The two ends of the bracket in the logistics transportation module B are respectively used for receiving the PCR plate containing the amplification reagent and the PCR plate containing the gene sample. During transportation, at the second switch door assembly A, the logistics transportation module A transfers the PCR plate containing the amplification reagent to the logistics transportation module B. At the second switch door assembly B, the logistics transportation module B transfers the PCR plate containing the gene sample to the logistics transportation module C. The lifting end of the lifting component B of the logistics transportation module C is located directly below the first switch door assembly C9.

[0111] The brackets of the logistics transport module A and the logistics transport module C are both provided with blocks, and the PCR plate and the sealing plate are both provided with grooves. The brackets of the logistics transport module A and the logistics transport module C achieve the purpose of receiving the PCR plate through the blocks and the grooves; the transfer process between the logistics transport module A and the logistics transport module B is as follows: the first end of the bracket of the logistics transport module B is moved to the second installation chamber A4 through the translation component D21 and the translation component E24, the bracket of the logistics transport module A receives the PCR plate, and the height of the bracket is raised through the lifting component B23 so that it is located above the bracket of the logistics transport module B, and then the PCR plate is moved through the translation component D21, and the bracket in the logistics transport module B is located directly below the bracket of the logistics transport module A, and then the height of the bracket is lowered through the lifting component B23 of the logistics transport module A, so that the PCR plate is transferred from the logistics transport module A to the logistics transport module B;

[0112] The transfer process between logistics transport module B and logistics transport module C is as follows: the lifting component B23 of logistics transport module C lowers the height of the bracket so that it is located below the bracket in logistics transport module B, and the second end of the bracket of logistics transport module C is moved to the second installation chamber C6 through the translation component D21 and the translation component E24, so that the second end of the bracket in logistics transport module B is located directly above the bracket of logistics transport module C, and then the height of the bracket is raised through the lifting component B23 of logistics transport module C, so that the PCR plate is transferred from logistics transport module B to logistics transport module C.

[0113] like Figure 22 As shown, the airflow control system includes three airflow components, which are respectively used to control the internal air pressure of the first installation chamber A1, the first installation chamber B2 and the first installation chamber C3, each airflow component includes an induced draft fan B and an induced draft fan C; the air outlet of the induced draft fan B is connected with the air inlet of the installation chamber, the air inlet of the induced draft fan C is connected with the air outlet of the installation chamber, and the air inlet of the induced draft fan B and the air inlet of the induced draft fan C are both provided with a high-efficiency filter B; the air pressure of the first installation chamber A1, the first installation chamber B2 and the first installation chamber C3 decreases in sequence, the air pressure of the first installation chamber A1 is greater than the air pressure outside the safety cabinet, and the air pressure of the first installation chamber C3 is less than the air pressure outside the safety cabinet.

[0114] In this embodiment, a high-efficiency filter B is provided at the air inlet of the induced draft fan B and the air inlet of the induced draft fan C to prevent air pollution. At the same time, the induced draft fan B and the induced draft fan C can both be speed-adjusted to control the pressure of the first installation chamber A1, the first installation chamber B2 and the first installation chamber C3. It is necessary to ensure that the pressure difference from the first installation chamber A1, the first installation chamber B2 and the first installation chamber C3 decreases by 5-20KPa in sequence, and the air pressure of the first installation chamber A1 is greater than the air pressure outside the safety cabinet, and the air pressure of the first installation chamber C3 is less than the air pressure outside the safety cabinet.

[0115] In addition, when testing high-risk viruses and other scenarios, it is necessary to prevent the air in the first installation chamber B2 inside the device from polluting the outside air to protect the safety of the testers. By adjusting the speeds of the induced draft fans B and C, the air pressure in the first installation chamber B2 is controlled to be less than the air pressure outside the safety cabinet. When the placement racks C1401, D1402, E1403, and F1404 are pulled out, outside air flows into the device through the openings of the first installation chamber B2. However, exhaust fans for extracting air are provided at the openings of the first installation chamber B2, and the exhaust fans extract the outside air, so the first installation chamber B2 will not be polluted. When testing scenarios such as high sensitivity, it is necessary to prevent the outside air from polluting the first installation chamber B2 inside the device. At this time, by adjusting the speeds of the induced draft fans B and C, the air pressure in the first installation chamber B2 is controlled to be greater than the air pressure outside the safety cabinet to prevent air from flowing in.

[0116] Such as Figure 1 、 Figure 3 、 Figure 10 As shown in

[0117] In this embodiment, when discharging waste from the replacement tips A and B, the translation assembly G2501 moves the cover plate 2502 to open the solid waste discharge chamber, and then the waste from the replacement tips A and B can be discharged into the solid waste discharge chamber. After the discharge is completed, the translation assembly G2501 moves the cover plate 2502 in the reverse direction to close the solid waste discharge chamber. When discharging liquid waste, the translation assembly G2501 moves the cover plate 2502 to open the liquid waste discharge chamber, and the liquid transfer member A1204 discharges the liquid waste into the liquid waste discharge chamber. After the discharge is completed, the translation assembly G2501 moves the cover plate 2502 in the reverse direction to close the solid waste discharge chamber.

[0118] Such as Figure 1 、 Figure 10 、 Figure 12As shown, the gene detection device further includes a heat-sealing housing 28 and a translation assembly H2804. The heat-sealing machine 2802 and the translation assembly H2804 are installed inside the heat-sealing housing 28. The heat-sealing housing 28 is provided with a third switch door assembly 2801. The translation assembly H2804 is used to move the film-sealing plate and the PCR plate to the lower end of the heating plate of the heat-sealing machine 2802. An installation plate F2803 is fixedly arranged at the moving action end of the translation assembly H2804. The PCR plate and the film-sealing plate are placed on the installation plate F2803. The installation plate F2803 is in pressing contact with the third switch door assembly 2801. The rebound direction of the third switch door assembly 2801 faces the heat-sealing machine 2802.

[0119] As Figure 1 shown, the gene detection device further includes a waste chamber, which is installed in the second installation chamber C6. A first switch door assembly D is provided between the first installation chamber C3 and the waste chamber.

[0120] As Figure 1 、 Figure 2 、 Figure 9 、 Figure 10 shown, the first switch door assembly A7, the first switch door assembly B8, the first switch door assembly C9, the first switch door assembly D, the second switch door assembly A10, and the second switch door assembly B11 all include a door panel and an elastic member. The two ends of the elastic member are respectively fixedly connected to the installation chamber and the first end of the door panel. When the elastic members of the first switch door assembly A7, the first switch door assembly B8, the first switch door assembly C9, and the first switch door assembly D recover from deformation, they drive the door panel to rotate upward. When the elastic members of the second switch door assembly A10 and the second switch door assembly B11 recover from deformation, they drive the door panel to rotate towards the second installation chamber B5.

[0121] In this embodiment, the transfer assembly 12 is used to control the downward movement of the jaw A16, so that the first switch door assembly A7, the first switch door assembly B8, the first switch door assembly C9, and the first switch door assembly D are opened, enabling the PCR plate to be transferred between the upper installation chamber and the lower installation chamber. After the transfer is completed, the transfer assembly 12 is used to control the upward movement of the jaw A16. Under the action of the elastic member, the first switch door assembly A7, the first switch door assembly B8, the first switch door assembly C9, and the first switch door assembly D can be automatically closed. The logistics transportation module B is used to open the second switch door assembly A10 and the second switch door assembly B11, enabling the PCR to be transferred between the three lower installation chambers. After the transfer is completed, the logistics transportation module B moves in a direction away from the second switch door assembly A10 or the second switch door assembly B11. Under the action of the elastic member, the second switch door assembly A10 and the second switch door assembly B11 can be automatically closed.

[0122] Among them, the translation component A1703, the translation component B2005, the translation component C1504, the translation component E24, the translation component F, the translation component G2501, the translation component H2804, and the translation component I30 are any one of a cylinder, an oil cylinder, a linear motor, a lead screw nut mechanism, and a rack and pinion.

[0123] The working process of the gene detection device of the present invention is as follows:

[0124] In the first installation chamber A1, the placement racks A1301 and B1302 are inserted. The sampling module changes the positions of the pipetting part A1204 in the X, Y, and Z directions through the transfer component 12, so as to transfer the amplification reagent into the micropores of the PCR plate. Then, the gripper control part A controls the gripper A16 to grasp the PCR plate containing the amplification reagent, and moves the PCR plate to be directly above the first switch door component A7 through the transfer component 12. Then, the transfer component 12 is used to control the gripper A16 to move downward, so that the first switch door component A7 opens, and the PCR plate is placed on the bracket 22 of the logistics transportation module A. Then, it is transported to the logistics transportation module B by the logistics transportation module A, and the logistics transportation module B transports the PCR plate to be directly below the first switch door component B8;

[0125] In the first installation chamber B2, the transfer assembly 12 moves the gripper A16 to be directly above the first switch door assembly B8. Then, the transfer assembly 12 controls the gripper A16 to move downward, opening the first switch door assembly B8, grasping the PCR plate into the first installation chamber B2, inserting the placement racks C1401, D1402, E1403, and F1404. The transfer assembly 12 moves the gripper C1205 to pick up the eight-well tube and place it at the transfer position 1411. The transfer assembly 12 moves the pipetting member A1204 for transferring the purification reagent, and transfers the corresponding purification reagent into the eight-well tube through the pipetting member A1204. Then, the transfer assembly 12 moves the gripper C1205 to pick up the sample tube and open the lid at the opening and closing assembly 20. Then, the gene sample in the sample tube is transferred into the eight-well tube through the pipetting member A1204 for transferring the gene sample before purification. Then, the eight-well tube is transferred to the purification module 17 for purification processing. After the purification processing is completed, the purified gene sample is transferred into the PCR plate through the pipetting member B29. Then, the gripper A transfers the PCR plate and the sealing film plate to the mounting plate F2803. The translation assembly H2804 moves the sealing film plate and the PCR plate to the lower end of the heating plate of the heat sealer 2802. The heat sealer 2802 heat-seals the sealing film plate and the PCR plate together. Finally, the gripper A transfers the heat-sealed PCR to be directly above the first switch door assembly B8. Then, the transfer assembly 12 controls the gripper A16 to move downward, opening the first switch door assembly B8, and placing the PCR plate at the second end of the bracket 22 in the logistics transportation module B. Then, it is transported to the logistics transportation module C by the logistics transportation module B. The transfer end of the logistics transportation module C is directly below the first switch door assembly C9;

[0126] In the first installation chamber C3, the transfer assembly 12 moves the gripper A16 to be directly above the first switch door assembly C9. Then, the transfer assembly 12 controls the gripper A16 to move downward, opening the first switch door assembly C9, grasping the PCR plate into the first installation chamber C3, and transferring the heat-sealed PCR plate to the heating base 1502. The translation assembly C1504 moves the heating base 1502 to move the PCR plate to be directly below the heat cover assembly 1503. Then, a metal bath is performed to amplify the gene sample. At the same time, the optical detector 1501 performs optical detection and analysis on the amplified gene sample. After the amplification detection is completed, the translation assembly C1504 moves the heating base 1502 to move the PCR plate out. Then, it is separated from the heating base 1502 through the transfer assembly 12 and the gripper A, and waste is discharged.

[0127] The technical solution of the present invention is not limited to the limitations of the above specific embodiments. Any technical deformation made according to the technical solution of the present invention falls within the protection scope of the present invention.

Claims

1. A gene detection device, characterized in that, Including: A safety cabinet, which includes an upper cabinet body and a lower cabinet body; three independent installation chambers are arranged in the upper cabinet body, namely a first installation chamber A, a first installation chamber B, and a first installation chamber C; a sampling module for adding amplification reagents into the micro-wells of the PCR plate is provided in the first installation chamber A; a purification processing module for processing gene samples is provided in the first installation chamber B; an amplification and detection module for gene amplification and detection is provided in the first installation chamber C; three communicable installation chambers and a logistics transportation module for transporting the PCR plate are arranged in the lower cabinet body; the installation chambers of the lower cabinet body of the safety cabinet are the second installation chamber A, the second installation chamber B, and the second installation chamber C respectively corresponding to the three installation chambers of the upper cabinet body, and a switch door assembly is provided between each installation chamber of the upper cabinet body and the corresponding installation chamber of the lower cabinet body, and between adjacent installation chambers of the lower cabinet body; An air flow control system; the air flow control system is used to control the air pressure conditions of the first installation chamber A, the first installation chamber B, and the first installation chamber C; The air flow control system includes three air flow components, and the three air flow components are respectively used to control the internal air pressure of the first installation chamber A, the first installation chamber B, and the first installation chamber C. Each air flow component includes an induced draft fan B and an induced draft fan C; the air outlet of the induced draft fan B is communicated with the air inlet of the installation chamber, the air inlet of the induced draft fan C is communicated with the air outlet of the installation chamber, and high-efficiency filters B are arranged at the air inlets of both the induced draft fan B and the induced draft fan C; the air pressures of the first installation chamber A, the first installation chamber B, and the first installation chamber C decrease in sequence, the air pressure of the first installation chamber A is greater than the air pressure outside the safety cabinet, and the air pressure of the first installation chamber C is less than the air pressure outside the safety cabinet; both the induced draft fan B and the induced draft fan C can be speed-regulated, and an exhaust fan for exhausting air is arranged at the opening of the first installation chamber B; when it is necessary to avoid the air pollution in the first installation chamber B from polluting the outside air, the air pressure of the first installation chamber B is controlled to be less than the air pressure outside the safety cabinet by speed-regulating the induced draft fan B and the induced draft fan C; When it is necessary to avoid the outside air from polluting the first installation chamber B inside the equipment, the air pressure of the first installation chamber B is controlled to be greater than the air pressure outside the safety cabinet by speed-regulating the induced draft fan B and the induced draft fan C.

2. The gene detection device according to claim 1, wherein The amplification and detection module includes a transfer component, a gripper A for transferring the PCR plate, and a gripper control part A for controlling the opening and closing of the gripper A.

3. The gene detection device according to claim 2, wherein, The transfer component includes: an X-direction moving component for moving in the X direction; the X-direction moving component includes a motor A, a driving wheel A, a driven wheel A, a transmission belt A, and a mounting plate A. The mounting plate A is fixedly connected to the transmission belt A. The rotating shaft of the motor A is fixedly connected to the rotation center of the driving wheel A. The driving wheel A and the driven wheel A are driven by the transmission belt A; a Y-direction moving component for moving in the Y direction; the Y-direction moving component includes a motor B, a driving wheel B, a driven wheel B, a transmission belt B, and a mounting plate B. The mounting plate B is fixedly connected to the transmission belt B. The rotating shaft of the motor B is fixedly connected to the rotation center of the driving wheel B. The driving wheel B and the driven wheel B are driven by the transmission belt B; a Z-direction moving component for moving in the Z direction; the Z-direction moving component includes a motor C, a lead screw-nut mechanism A, and a mounting plate C. The rotating shaft of the motor C is fixedly connected to the lead screw A of the lead screw-nut mechanism A. The mounting plate C is fixedly connected to the nut A of the lead screw-nut mechanism A. Wherein, the X-direction moving component is installed on the mounting plate B, and the Z-direction moving component is fixedly installed on the mounting plate A.

4. The gene detection device according to claim 1, characterized in that A first switch door assembly C is provided between the first installation chamber C and the second installation chamber C, and a logistics transportation module C is provided in the second installation chamber C.

5. The gene detection device according to claim 4, characterized in that, The logistics transportation module C includes a bracket for receiving the PCR plate and a lifting component B for lifting the height of the bracket.

6. The gene detection device according to claim 5, wherein A clamping block is provided on the bracket of the logistics transportation module C, and the clamping block is adapted to the groove of the PCR plate carried on the bracket.

7. The gene detection device according to claim 1, characterized in that, The second installation chamber C further includes a waste chamber, and a switch door assembly is provided between the first installation chamber C and the waste chamber.

8. The gene detection device according to any one of claims 1 to 7, characterized in that, The amplification detection module further includes at least two sets of metal bath modules, at least two sets of optical detectors, and at least two sets of translation components C. Each metal bath module includes a thermal cover component and a heating base. One set of the thermal cover components is installed at the detection port of one set of the optical detectors, and the moving action end of one set of the translation components C acts on one of the heating bases.

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