An improved food immune detection device and its detection method

Abstract

This invention discloses an improved food immunoassay device, comprising a test chamber, a tester, a paper-changing mechanism, a test cover, an incubator, a temperature control mechanism, a sample storage box, a clamping mechanism, a box lid, a control panel, and a buckle. The tester is slidably connected inside the test chamber, and the paper-changing mechanism is located inside the tester. The test cover is hinged to the tester. The incubator is fixedly connected inside the test chamber, and the temperature control mechanism is located inside the incubator. The sample storage box is fixedly connected inside the test chamber, and the clamping mechanism is located inside the sample storage box, which is in contact with the incubator. The box lid is hinged to the test chamber, and the control panel is located on the box lid. A buckle is fixedly connected to the box lid, and the buckle contacts the test chamber. This invention relates to an improved food immunoassay device and its detection method, featuring easy test paper replacement and the ability to cultivate bacterial flora at a constant temperature.

Description

Technical Field

[0001] This invention belongs to the field of food immune detection technology, specifically an improved food immune detection device and its detection method. Background Technology

[0002] In industries closely related to people's daily lives, food safety is of utmost concern to the general public. Whether or not food is safe directly affects people's health and even their lives. To ensure food safety, national food safety supervision departments conduct random sampling tests on food circulating in the market from time to time, especially for imported foods, which need to be tested for drug residues and mycotoxins. However, current testing equipment has some problems: 1. Test strips are inconvenient to replace, especially when testing a large number of samples, affecting testing efficiency; 2. When testing for fungi, the heating effect on the petri dish is poor, resulting in incomplete bacterial cultures and affecting test results. Therefore, it is necessary to design an improved food immunoassay device and its detection method. Summary of the Invention

[0003] The purpose of this invention is to provide an improved food immune detection device and detection method to solve the above-mentioned problems, thereby resolving the issues mentioned in the background art.

[0004] To address the above problems, the present invention provides a technical solution for an improved food immune detection device and detection method:

[0005] An improved food immunoassay device includes a test chamber, a tester, a paper-changing mechanism, a test cover, an incubator, a temperature control mechanism, a sample storage box, a clamping mechanism, a box lid, a control panel, and a buckle. The tester is slidably connected inside the test chamber, and the tester has a paper-changing mechanism inside. The test cover is hinged to the tester. The incubator is fixedly connected inside the test chamber, and the temperature control mechanism is inside the incubator. The sample storage box is fixedly connected inside the test chamber, and the sample storage box has a clamping mechanism inside. The sample storage box is in contact with the incubator. The box lid is hinged to the test chamber, and the control panel is located on the box lid. A buckle is fixedly connected to the box lid, and the buckle is in contact with the test chamber. Both the incubator and the sample storage box are in contact with the box lid.

[0006] Preferably, a conductive sponge is fixedly connected inside the testing box, and the conductive sponge is in contact with the testing instrument.

[0007] Preferably, the paper changing mechanism includes a changing frame, a paper roller, test paper, a limiting frame, a grooved wheel, a limiting rod, a baffle, a spring, a connecting rope, a guide wheel, a support plate, a limiting block, a guide roller, and a limiting rail. The changing frame is bolted to the inside of the tester. The paper roller is rotatably connected to the inside of the changing frame. Test paper is wound around the outside of the paper roller. The limiting frame is fixedly connected to the inside of the tester. A grooved wheel is fixedly connected to the paper roller. A limiting rod is slidably connected to the inside of the limiting frame. A baffle is fixedly connected to the outside of the limiting rod. A spring is provided on the outside of the limiting rod. A connecting rope is fixedly connected to the limiting rod. A support plate is fixedly connected to the inside of the tester. A limiting block is slidably connected to the support plate. The limiting block is fixedly connected to the connecting rope. A limiting rail is fixedly connected to the support plate. A guide roller is rotatably connected to the inside of the support plate. The test paper is in contact with the detection cover, the guide roller, and the limiting rail.

[0008] Preferably, one end of the spring is fixedly connected to the baffle, and the other end of the spring is fixedly connected to the limiting frame.

[0009] Preferably, a guide wheel is fixedly connected inside the tester, and the guide wheel is in contact with the connecting rope.

[0010] Preferably, the constant temperature mechanism includes a geared motor, a clamping plate, a magnet, a loading rack, a heating element, a heat-conducting cover, a loading plate, a clamping block, a spring, and a guide block. The geared motor is fixedly connected inside the incubator. The clamping plate is mounted inside the incubator via bearings. The clamping plate is fixedly connected to the output shaft of the geared motor. A magnet is fixedly connected to the clamping plate. A heating element is fixedly connected inside the incubator. A heat-conducting cover is fixedly connected inside the incubator. The loading rack is attracted to the magnet and contacts the heat-conducting cover. A loading plate is fixedly connected inside the loading rack. A clamping block is slidably connected inside the loading plate. A guide block is fixedly connected to the clamping block and slidably connected to the loading plate. A spring is fixedly connected to the loading plate and contacts the clamping block.

[0011] Preferably, the clamping mechanism includes guide rods, a petri dish, an elastic band, a drive seat, and a limiting piece. Two symmetrically distributed guide rods are fixedly connected inside the sample storage box. A petri dish is slidably connected inside the sample storage box. An elastic band is provided inside the sample storage box, with one end fixedly connected to the sample storage box. A drive seat is slidably connected to the outside of the guide rods, and the other end of the elastic band is fixedly connected to the drive seat. A limiting piece is slidably connected inside the drive seat. Both the limiting piece and the drive seat are in contact with the petri dish.

[0012] An improved detection method for food immune detection devices, comprising the following specific steps:

[0013] Step 1: Take a certain amount of the food to be tested, divide the food into small pieces, then mix the food with the extract, and after mixing, use a dropper to extract a small amount of the mixture.

[0014] Step 2: Disconnect the buckle from the testing box, then open the box cover and the testing cover. Pull the limiting block, which, through the connecting rope, moves the limiting rod in the direction of disengaging from the grooved wheel and presses the spring. When the limiting rod disengages from the grooved wheel, the test paper can be pulled. The test paper drives the paper roller to rotate, allowing the test paper to be pulled out. After pulling it out, release the limiting block, and the spring will return to its original position. The spring can then use its elasticity to drive the limiting rod to slide into the grooved wheel to limit the paper roller. Then, place the test paper into the limiting rail, drop the mixed liquid onto the test paper, fold the test paper, and place the testing cover on the testing instrument to detect pesticide and veterinary drug residues inside the food.

[0015] Step 3: Push the limiting plate into the drive seat. Once the limiting plate is detached from the culture dish, the culture dish can be removed. Then, drop the mixture into the inside of the culture dish, remove the loading rack, move the clamping block to compress the spring, place the culture dish on the loading plate and release the clamping block. The spring returns to its original position, and the spring, through its elasticity, drives the clamping block to limit the culture dish. Then, place the loading rack on the magnet block and simultaneously start the heating element and the geared motor. The heating element can transfer heat to the culture dish through the heat conduction cover. During the heating process, the geared motor can drive the culture dish inside the loading rack to rotate through the clamping plate, so that the culture dish is heated evenly and the bacteria inside the mixture can be fully heated and grown.

[0016] Step 4: After the test paper has been heated for a certain time, remove the comparison card and compare the color of the test paper with the residual concentration on the test card. After the bacterial community inside the petri dish has been cultured, the bacterial community can be observed under a microscope.

[0017] The beneficial effects of this invention are as follows: This invention relates to an improved food immunoassay device and its detection method, which features easy replacement of test strips and the ability to cultivate bacterial flora at a constant temperature. In practical use, compared with traditional improved food immunoassay devices and their detection methods, this improved food immunoassay device and its detection method have the following two beneficial effects:

[0018] First, by adding structures such as paper rollers, grooved wheels and limiting rods to the tester, the test paper strips that were originally single sheets can be replaced by paper rollers. During testing, the test strips only need to be pulled out to the appropriate length to be placed on the limiting rail, thus avoiding the problem of inconvenience in replacing single sheets.

[0019] Secondly, by adding a geared motor, loading rack, and heat-conducting cover inside the incubator, the culture dish can be clamped on the loading plate and placed on the clamping plate during use. During the culture process, the geared motor can drive the culture dish to rotate slowly, which can make the culture dish heat more evenly and ensure the accuracy of the test results. Attached Figure Description

[0020] For ease of explanation, the present invention will be described in detail below with reference to specific embodiments and accompanying drawings.

[0021] Figure 1 This is a perspective view of the overall structure of the present invention;

[0022] Figure 2 For the present invention Figure 1 A front sectional view;

[0023] Figure 3 For the present invention Figure 2 Left sectional view of the testing instrument;

[0024] Figure 4 For the present invention Figure 2 A cross-sectional view of the incubator;

[0025] Figure 5 For the present invention Figure 4 A cross-sectional view of the loading rack;

[0026] Figure 6 For the present invention Figure 2 A cross-sectional view of the sample storage box;

[0027] Figure 7 For the present invention Figure 3 Enlarged view of the A-section structure;

[0028] Figure 8 For the present invention Figure 5 Enlarged view of the structure of section B;

[0029] Figure 9 For the present invention Figure 6 Enlarged view of the C-section structure.

[0030] In the diagram: 1. Testing box; 2. Tester; 3. Paper changing mechanism; 4. Testing cover; 5. Incubator; 6. Temperature control mechanism; 7. Sample storage box; 8. Clamping mechanism; 9. Box lid; 10. Control panel; 11. Buckle; 12. Conductive sponge; 31. Changing frame; 32. Paper roller; 33. Test paper; 34. Limiting frame; 35. Grooved wheel; 36. Limiting rod; 37. Baffle; 38. Spring; 39. Connecting rope; 0. Guide wheel; 301. Support plate; 302. Limiting block; 303. Guide roller; 304. Limiting rail; 61. Gear motor; 62. Clamping plate; 63. Magnet block; 64. Loading rack; 65. Heating tube; 66. Heat conducting cover; 67. Loading plate; 68. Clamping block; 69. Spring; 60. Guide block; 81. Guide rod; 82. Petri dish; 83. Elastic band; 84. Drive seat; 85. Limiting piece. Detailed Implementation

[0031] like Figure 1-9 As shown, the specific implementation adopts the following technical solution:

[0032] Example:

[0033] An improved food immunoassay device includes a test chamber 1, a tester 2, a paper changing mechanism 3, a test cover 4, an incubator 5, a temperature control mechanism 6, a sample storage box 7, a clamping mechanism 8, a box cover 9, a control panel 10, and a buckle 11. The tester 2 is slidably connected inside the test chamber 1. The paper changing mechanism 3 is located inside the tester 2. The test cover 4 is hinged to the tester 2. The incubator 5 is fixedly connected inside the test chamber 1. The temperature control mechanism 6 is located inside the incubator 5. The sample storage box 7 is fixedly connected inside the test chamber 1. The clamping mechanism 8 is located inside the sample storage box 7, which is in contact with the incubator 5. The box cover 9 is hinged to the test chamber 1. The control panel 10 is located on the box cover 9. The buckle 11 is fixedly connected to the box cover 9 and is in contact with the test chamber 1. Both the incubator 5 and the sample storage box 7 are in contact with the box cover 9.

[0034] The testing box 1 contains a fixed conductive sponge 12, which is in contact with the testing instrument 2. The conductive sponge 12 is produced using polymer composite material foaming technology. It has uniform pore size, is soft and elastic, protects the testing instrument 2, and is corrosion-resistant, making it an ideal medium material for long-term device storage.

[0035] The paper changing mechanism 3 includes a changing frame 31, a paper roller 32, a test paper 33, a limiting frame 34, a grooved wheel 35, a limiting rod 36, a baffle 37, a spring 38, a connecting rope 39, a guide wheel 30, a support plate 301, a limiting block 302, a guide roller 303, and a limiting rail 304. The changing frame 31 is bolted to the inside of the testing instrument 2. The paper roller 32 is rotatably connected to the inside of the changing frame 31. The test paper 33 is wound around the outside of the paper roller 32, and the paper roller 32 rewinds the test paper 33. The limiting frame 34 is fixedly connected to the inside of the testing instrument 2. A grooved wheel 35 is fixedly connected to the paper roller 32. The limiting rod 36 is slidably connected to the inside of the limiting frame 34. 6. A baffle 37 is fixedly connected to the outer side of the limiting rod 36. A spring 38 is provided on the outer side of the limiting rod 36. A connecting rope 39 is fixedly connected to the limiting rod 36. The connecting rope 39 can be flexible and can drive the limiting rod 36 to move through the guide wheel 30. A support plate 301 is fixedly connected inside the testing instrument 2. A limiting block 302 is slidably connected to the support plate 301. The limiting block 302 is fixedly connected to the connecting rope 39. A limiting rail 304 is fixedly connected to the support plate 301. A guide roller 303 is rotatably connected inside the support plate 301. The test paper 33 is in contact with the detection cover 4, the guide roller 303 and the limiting rail 304. By designing the paper changing mechanism 3, when changing the paper, the test paper 33 is pulled out. The test paper 33 drives the paper roller 32 to rotate, so that the test paper 33 can be pulled out and the test paper 33 inside the limiting rail 304 can be replaced.

[0036] One end of the spring 38 is fixedly connected to the baffle 37, and the other end of the spring 38 is fixedly connected to the limiting frame 34. The spring 38 can automatically reset the limiting rod 36 through its elastic force, thereby automatically limiting the paper roller 32.

[0037] The testing instrument 2 has a guide wheel 30 fixedly connected inside, and the guide wheel 30 is in contact with the connecting rope 39. The guide wheel 30 is designed to guide and change the direction of force transmission in the connecting rope 39.

[0038] The constant temperature mechanism 6 includes a geared motor 61, a clamping plate 62, a magnet 63, a loading rack 64, a heating element 65, a heat-conducting cover 66, a loading plate 67, a clamping block 68, a spring 69, and a guide block 60. The geared motor 61 is fixedly connected inside the incubator 5. The geared motor 61 is existing technology and provides driving force. The clamping plate 62 is mounted inside the incubator 5 via bearings. The clamping plate 62 is fixedly connected to the output shaft of the geared motor 61. A magnet 63 is fixedly connected to the clamping plate 62. The heating element 65, existing technology, is fixedly connected inside the incubator 5 and is used for heating. A heat-conducting cover 66 is fixedly connected inside the container. A loading rack 64 is attached to the magnet block 63. The loading rack 64 is made of iron material and can be attracted by the magnet block 63. The loading rack 64 is in contact with the heat-conducting cover 66. A loading plate 67 is fixedly connected inside the loading rack 64. A clamping block 68 is slidably connected inside the loading plate 67. A guide block 60 is fixedly connected to the clamping block 68. The guide block 60 is slidably connected to the loading plate 67. A spring piece 69 is fixedly connected to the loading plate 67. The spring piece 69 can push the clamping block 68 to limit the position of the culture dish 82 through elastic force. The spring piece 69 is in contact with the clamping block 68. By designing a constant temperature mechanism 6, the loading rack 64 is removed, the clamping block 68 is moved to compress the spring 69, the culture dish 82 is placed on the loading plate 67 and the clamping block 68 is released, the spring 69 is reset, and the spring 69 drives the clamping block 68 to limit the culture dish 82 through the elastic force. Then the loading rack 64 is placed on the magnet block 63, and the electric heating tube 65 and the geared motor 61 are started at the same time. The electric heating tube 65 can transfer heat to the culture dish 82 through the heat conduction cover 66 to achieve the purpose of uniform heating.

[0039] The clamping mechanism 8 includes a guide rod 81, a petri dish 82, an elastic band 83, a drive seat 84, and a limiting piece 85. Two symmetrically distributed guide rods 81 are fixedly connected inside the sample storage box 7. The petri dish 82 is slidably connected inside the sample storage box 7. An elastic band 83 is provided inside the sample storage box 7, with one end fixedly connected to the sample storage box 7. The drive seat 84 is slidably connected to the outer side of the guide rods 81, and the other end of the elastic band 83 is fixedly connected to the drive seat 84. A limiting piece 85 is slidably connected inside the drive seat 84. The limiting piece 85 can slide into the drive seat 84 when the petri dish 82 is picked up, making it easier to pick up. Both the limiting piece 85 and the drive seat 84 are in contact with the petri dish 82. By designing a clamping mechanism 8, the elastic band 83 inside the clamping mechanism 8 drives the limiting piece 85 to press the culture dish 82. By pushing the limiting piece 85 into the drive seat 84, the culture dish 82 can be taken out after the limiting piece 85 is removed from the culture dish 82.

[0040] An improved detection method for food immune detection devices, comprising the following specific steps:

[0041] Step 1: Take a certain amount of the food to be tested, divide the food into small pieces, then mix the food with the extract, and after mixing, use a dropper to extract a small amount of the mixture.

[0042] Step 2: Disconnect buckle 11 from test box 1, then open box cover 9, open test cover 4, pull limit block 302, limit block 302 drives limit rod 36 to move in the direction of disengaging from groove wheel 35 through connecting rope 39, and press spring 38. When limit rod 36 disengages from groove wheel 35, test paper 33 can be pulled. Test paper 33 drives paper roller 32 to rotate, so test paper 33 can be pulled out. After pulling out, release limit block 302, spring 38 returns to its original position. Spring 38 can drive limit rod 36 to slide into the inside of groove wheel 35 through elastic force to limit paper roller 32. Then put test paper 33 into the inside of limit rail 304, drip the mixed liquid onto test paper 33, fold test paper 33, and put test cover 4 on tester 2 to detect pesticide and veterinary drug residues inside food.

[0043] Step 3: Push the limiting piece 85 into the drive seat 84. After the limiting piece 85 is disengaged from the culture dish 82, the culture dish 82 can be removed. Then, drip the mixture into the inside of the culture dish 82. Remove the loading rack 64, move the clamping block 68 to compress the spring piece 69, place the culture dish 82 onto the loading plate 67, and release the clamping block 68. The spring piece 69 returns to its original position. The spring piece 69 uses its elasticity to limit the clamping block 68 and the culture dish 82. Then, place the loading rack 64 on the magnet block 63 and simultaneously start the electric heating tube 65 and the geared motor 61. The electric heating tube 65 can transfer heat to the culture dish 82 through the heat conduction cover 66. During the heating of the culture dish 82, the geared motor 61 can drive the culture dish 82 inside the loading rack 64 to rotate through the clamping plate 62, so that the culture dish 82 is heated evenly and the bacteria inside the mixture can be fully heated and grown.

[0044] Step 4: After the test paper 33 has been heated for a certain time, take out the comparison card and compare the color of the test paper 33 with the residual concentration on the test card. After the bacterial community inside the culture dish 82 has been cultured, the bacterial community can be observed under a microscope.

[0045] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the present invention. Various changes and modifications can be made to the present invention without departing from its spirit and scope. All such changes and modifications fall within the scope of the present invention as claimed, which is defined by the appended claims and their equivalents.

Claims

1. An improved food immunoassay device, comprising a test chamber (1), a tester (2), a paper changing mechanism (3), a test cover (4), an incubator (5), a constant temperature mechanism (6), a sample storage box (7), a clamping mechanism (8), a box cover (9), a control panel (10), and a buckle (11), characterized in that: The inside of the test box (1) is slidably connected to a tester (2), the inside of the tester (2) is provided with a paper changing mechanism (3), the tester (2) is hinged with a test cover (4), the inside of the test box (1) is fixedly connected to an incubator (5), the inside of the incubator (5) is provided with a constant temperature mechanism (6), the inside of the test box (1) is fixedly connected to a sample storage box (7), the inside of the sample storage box (7) is provided with a clamping mechanism (8), the sample storage box (7) is in contact with the incubator (5), the test box (1) is hinged with a box cover (9), the box cover (9) is provided with a control panel (10), the box cover (9) is fixedly connected with a buckle (11), the buckle (11) is in contact with the test box (1), the incubator (5) and the sample storage box (7) are both in contact with the box cover (9); The paper changing mechanism (3) includes a changing frame (31), a paper roller (32), a test paper (33), a limiting frame (34), a grooved wheel (35), a limiting rod (36), a baffle (37), a spring (38), a connecting rope (39), a guide wheel (30), a support plate (301), a limiting block (302), a guide roller (303), and a limiting rail (304). The changing frame (31) is bolted to the inside of the tester (2). The paper roller (32) is rotatably connected inside the changing frame (31). The test paper (33) is wound around the outside of the paper roller (32). The limiting frame (34) is fixedly connected inside the tester (2). The grooved wheel (35) is fixedly connected to the paper roller (32). The limiting rod (36) is slidably connected inside the limiting frame (34). The baffle (37) is fixedly connected to the outside of the limiting rod (36). A spring (38) is provided on the outside of the device. A connecting rope (39) is fixedly connected to the limiting rod (36). A support plate (301) is fixedly connected inside the tester (2). A limiting block (302) is slidably connected to the support plate (301). The limiting block (302) is fixedly connected to the connecting rope (39). A limiting rail (304) is fixedly connected to the support plate (301). A guide roller (303) is rotatably connected inside the support plate (301). The test paper (33) is in contact with the detection cover (4), and the guide roller (303) is in contact with the limiting rail (304). One end of the spring (38) is fixedly connected to the baffle (37), and the other end of the spring (38) is fixedly connected to the limiting frame (34). A guide wheel (30) is fixedly connected inside the tester (2). The guide wheel (30) is in contact with the connecting rope (39).

2. The improved food immune detection device according to claim 1, characterized in that: A conductive sponge (12) is fixedly connected inside the testing box (1), and the conductive sponge (12) is in contact with the testing instrument (2).

3. The improved food immune detection device according to claim 1, characterized in that: The constant temperature mechanism (6) includes a geared motor (61), a clamping plate (62), a magnet (63), a loading rack (64), an electric heating tube (65), a heat-conducting cover (66), a loading plate (67), a clamping block (68), a spring (69), and a guide block (60). The geared motor (61) is fixedly connected inside the incubator (5). The clamping plate (62) is mounted inside the incubator (5) via bearings. The clamping plate (62) is fixedly connected to the output shaft of the geared motor (61). A magnet (63) is fixedly connected to the clamping plate (62). An electric heating tube is fixedly connected inside the incubator (5). The tube (65) has a heat-conducting cover (66) fixedly connected inside the incubator (5). A loading rack (64) is adsorbed on the magnet block (63). The loading rack (64) is in contact with the heat-conducting cover (66). A loading plate (67) is fixedly connected inside the loading rack (64). A clamping block (68) is slidably connected inside the loading plate (67). A guide block (60) is fixedly connected on the clamping block (68). The guide block (60) is slidably connected to the loading plate (67). A spring piece (69) is fixedly connected on the loading plate (67). The spring piece (69) is in contact with the clamping block (68).

4. An improved food immune detection device according to claim 1, characterized in that: The clamping mechanism (8) includes a guide rod (81), a petri dish (82), an elastic band (83), a drive seat (84), and a limiting piece (85). The sample storage box (7) has two symmetrically distributed guide rods (81) fixedly connected inside. The sample storage box (7) has a petri dish (82) slidably connected inside. The sample storage box (7) has an elastic band (83) inside. One end of the elastic band (83) is fixedly connected to the sample storage box (7). The drive seat (84) is slidably connected to the outside of the guide rod (81). The other end of the elastic band (83) is fixedly connected to the drive seat (84). The limiting piece (85) is slidably connected inside the drive seat (84). Both the limiting piece (85) and the drive seat (84) are in contact with the petri dish (82).

5. The detection method of the improved food immune detection device according to any one of claims 1-4, characterized in that: The specific steps are as follows: Step 1: Take a certain amount of the food to be tested, divide the food into small pieces, then mix the food with the extract, and after mixing, use a dropper to extract a small amount of the mixture. Step 2: Disconnect the buckle (11) from the test box (1), then open the box cover (9), and open the test cover (4). Pull the limiting block (302). The limiting block (302) drives the limiting rod (36) to move in the direction of disengaging from the grooved wheel (35) via the connecting rope (39), and presses the spring (38). When the limiting rod (36) disengages from the grooved wheel (35), the test paper (33) can be pulled. The test paper (33) drives the paper roller (32) to rotate, and the test paper can be pulled. The paper (33) is pulled out. After pulling it out, the limiting block (302) is released and the spring (38) is reset. The spring (38) can drive the limiting rod (36) to slide into the groove wheel (35) to limit the paper roller (32) through the elastic force. Then, the test paper (33) is placed into the limiting rail (304) and the mixed liquid is dripped onto the test paper (33). After folding the test paper (33), the detection cover (4) is placed on the tester (2) to detect the pesticide and veterinary drug residues inside the food. Step 3: Push the limiting piece (85) into the drive seat (84). Once the limiting piece (85) is detached from the culture dish (82), the culture dish (82) can be removed. Then, drop the mixture into the inside of the culture dish (82), remove the loading rack (64), and move the clamping block (68) to compress the spring piece (69). Place the culture dish (82) onto the loading plate (67) and release the clamping block (68). The spring piece (69) resets, and the spring piece (69) uses its elasticity to move the clamping block (68) and the culture dish (82) together. Then, the loading rack (64) is placed on the magnet block (63), and the electric heating tube (65) and the geared motor (61) are started at the same time. The electric heating tube (65) can transfer heat to the petri dish (82) through the heat conduction cover (66). During the heating of the petri dish (82), the geared motor (61) can drive the petri dish (82) inside the loading rack (64) to rotate through the clamping plate (62), so that the petri dish (82) is heated evenly and the bacteria inside the mixture can be fully heated and grown. Step 4: After the test paper (33) has been heated for a certain time, take out the comparison card and compare the color of the test paper (33) with the residual concentration on the test card. After the bacterial community inside the culture dish (82) has been cultured, the bacterial community can be observed through a microscope.

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