A glucose detection granule for cats and glucose indicating cat litter and a method of preparation

Abstract

The application discloses a glucose detection granule for cats, a glucose indicating cat litter and a preparation method. A master granule of a fiber material is used as a carrier, a glucose test solution is uniformly mixed with the master granule at a weight ratio of 1:5-2:1, and then is placed for 1-15 min, and then is dried at 35-65 DEG C for the first time until the water content is below 10%, and then a surface is sprayed with a coating agent solution, and then is dried at 35-65 DEG C for the second time until the water content is below 10% to obtain the glucose detection granule for cats. The application can stably and long-actingly play a role in the air, and the detection function is not affected after being mixed with various cat litters, and the detection effect is good.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pet product production, in particular to a glucose detection granule for cats, a glucose indicating cat litter and a preparation method. BACKGROUND

[0002] Clinically, the urine examination of cats is a routine examination, and the main detection items include urine turbidity, color, protein, pH, occult blood, glucose, ketone body, etc. Pet owners need to go to a pet hospital for special examination every day, and it is not convenient to take urine from pets, which is not conducive to timely detection of the disease condition of pets.

[0003] The existing technology has not involved glucose which has high clinical significance in the routine detection items. The reasons are as follows: first, the detection method involves an enzyme reaction, and the material is easy to lose activity and is easy to affect the performance during long-term use and storage; second, there are many types of cat litter, especially some currently popular tofu cat litter and the like which are rich in starch and the like, and the detected substance glucose may be decomposed due to the same, thereby causing higher challenges to the detection of glucose in cat litter.

[0004] CN201410742682.3 discloses a urine glucose content detection kit, which comprises the following components: buffer, glucose oxidase, dehydrogenase, o-tolidine, anti-interference agent and diluent. The urine glucose content detection kit is only suitable for human detection and is not suitable for cat detection. SUMMARY

[0005] The present application aims to provide a glucose detection granule for cats, a glucose indicating cat litter and a preparation method, which can stably and long-acting function in the air, and the detection function is not affected after being mixed with various cat litters, and the detection effect is good.

[0006] The technical solution adopted by the present application to solve the technical problems is as follows:

[0007] A glucose detection granule for cats, which is prepared by mixing a glucose test solution with a master granule of a fiber material as a carrier at a weight ratio of 1:5-2:1, standing for 1-15 min, and then drying at 35-65 DEG C to a water content of less than 10% for the first time, followed by spraying a coating agent solution on the surface and drying at 35-65 DEG C for the second time to a water content of less than 10% to obtain the granule. The glucose detection granule can react with glucose in cat urine to produce color, and the color starts to appear in 3 seconds at the fastest, the higher the glucose concentration, the deeper the color, and when the concentration is about 20 g / L, the color will not continue to deepen.

[0008] Cat urine collection is difficult and has great uncertainty, in order to solve the difficulty of detecting glucose in cat urine, the present application makes an innovation, by making a detection particle which can be mixed with cat litter, and is not affected by cat litter, and can be mixed with various cat litter, after cat naturally urinates on cat litter, urine contacts glucose detection particle, glucose detection particle will quickly produce color reaction, thereby playing a role of qualitative detection.

[0009] In order to make the detection particle suitable for mixing with cat litter without interference and long-acting effect, the present application specifically adds a wrapping agent, the role of the wrapping agent is to protect the enzymes in the particle, and can form a protective film to wrap the particle containing the glucose detection reagent, effectively isolate the contact between different cat litter and the detection particle when mixed with different cat litter; when the cat litter is exposed to air during use, it effectively isolates oxygen, moisture and other detection particles in the air, thereby prolonging the effect of the detection particle and ensuring the effectiveness of the detection. When the detection particle contacts cat urine, the wrapping agent will be dissolved by cat urine, and then contact the internal glucose detection reagent to react.

[0010] The present application uses inert fiber particles, and glucose detection solution is prepared by soaking and drying to obtain glucose detection particles, and after adding wrapping and isolation protective substances, it is mixed with various cat litter to obtain good detection effect without affecting the long-term detection performance.

[0011] Mix the glucose test solution and the master batch in a weight ratio of 1:5-2:1, stand for 1-15 min, and then dry at 35-65 DEG C to a water content of less than 10%; further more preferably, mix the glucose test solution and the master batch in a weight ratio of 1:2-1:0.7, stand for 1-15 min, and then dry at 40-55 DEG C to a water content of less than 10%.

[0012] The master batch as a whole is light-colored, such as light yellow, and the fiber material of the master batch can be subjected to decoloring treatment, and the color reaction is purple red, so that the observation effect is better.

[0013] As a preferred, the wrapping agent is selected from one or more of gelatin, gum arabic, sodium carboxymethyl cellulose, polyvinyl alcohol and hydroxyethyl cellulose.

[0014] As a preferred, the mass concentration of the wrapping agent solution is 0.1-5%.

[0015] As a preferred, the glucose test solution comprises:

[0016] Glucose oxidase 5-35 ku / L,

[0017] Peroxidase 3-50 ku / L,

[0018] 4-aminoantipyrine 0.1-1.5 wt%,

[0019] p-hydroxybenzoic acid or sodium 4-hydroxybenzoate 0.1-1.5wt%,

[0020] buffer the rest.

[0021] Further preferably, the glucose test solution is composed of:

[0022] glucose oxidase 15-25 ku / L,

[0023] peroxidase 15-25 ku / L,

[0024] 4-aminoantipyrine 0.5-1.2wt%,

[0025] p-hydroxybenzoic acid or sodium 4-hydroxybenzoate 0.3-1wt%,

[0026] buffer the rest.

[0027] As preferred, the pH of the buffer is 6.0-8.5, and the buffer is selected from one of 0.01-0.5mol / L Tris-HCL buffer, 0.03-0.3mol / L Good's buffer, 0.04-0.4mol / L HEPES buffer, 0.05-0.4mol / L borate buffer.

[0028] As preferred, the fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, polyacrylonitrile fiber; the length of the fiber is controlled to be 1-25mm.

[0029] As preferred, the master batch is treated by diatomite, specifically: the master batch is mixed with 0.05-1.0wt% nanoscale white diatomite suspension in a ratio of 1g:100-2000mL, ultrasonic mixing for 1-10 minutes, filtration, and drying. The master batch treated by diatomite has better adsorption to the glucose test solution and is more stable. In actual application, the color reaction and display are more persistent and bright.

[0030] As preferred, the master batch contains superabsorbent resin, and the content of the superabsorbent resin is 0.5-10%, and the content of the fiber material is 90-99.5%. As an optimized scheme, the present application adds superabsorbent resin in the master batch, which can increase the ability of the detection particles to bind and absorb the glucose test solution, and the water absorption is reversible in the drying process. In actual use, the absorption capacity of urine can be increased, and the detection effect is strengthened without affecting the clumping of cat litter.

[0031] A preparation method of a glucose detection particle for cats, comprising the following steps:

[0032] (1) Glucose oxidase, peroxidase, 4-aminoantipyrine and p-hydroxybenzoic acid or sodium 4-hydroxybenzoate are added into a buffer solution and stirred to obtain a glucose test solution;

[0033] (2) The coating agent is added into deionized water and stirred to obtain a coating agent solution;

[0034] (3) The glucose test solution and the master batch are mixed at a weight ratio of 1:5-2:1, and then left to stand for 1-15 min, and then dried at one time at a temperature of 35-65 ℃ until the water content is below 10% to obtain primary reaction granules;

[0035] (4) The coating agent solution is sprayed on the surface of the primary reaction granules, and then dried at two times at a temperature of 35-65 ℃ until the water content is below 10% to obtain glucose detection granules for cats.

[0036] A glucose indicating cat litter is formed by mixing the glucose detection granules for cats and cat litter at a weight ratio of 1:100-500. The granules and cat litter are mixed at a certain ratio to obtain cat litter that can be used daily and has a glucose detection function.

[0037] The present application has the advantages that it can stably and long-actingly function in the air, and the detection function is not affected after being mixed with various cat litters, and the detection effect is good. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 is a color development effect diagram of the glucose detection granules prepared in Example 1 of the present application forming an indicating cat litter;

[0039] Figure 2 is a color development effect diagram of the glucose detection granules prepared in Example 2 of the present application forming an indicating cat litter;

[0040] Figure 3 is a color development effect diagram of the glucose detection granules prepared in Example 3 of the present application forming an indicating cat litter. DETAILED DESCRIPTION

[0041] The technical solutions of the present application are further described in detail below through specific examples.

[0042] In the present application, the raw materials and equipment used, unless specified, can be purchased from the market or are commonly used in the art. The methods in the following examples, unless specified, are conventional methods in the art.

[0043] OVERALL EMBODIMENT

[0044] A glucose detection granule for cat, taking a master granule of fiber material as carrier, mixing glucose test solution with the master granule at a weight ratio of 1:5-2:1, standing for 1-15 min, then once-drying at 35-65℃ to a water content of below 10%, then spraying a coating agent solution on the surface and twice-drying at 35-65℃ to a water content of below 10% to obtain.

[0045] The coating agent is selected from one or more of gelatin, gum arabic, sodium carboxymethyl cellulose, polyvinyl alcohol, hydroxyethyl cellulose.

[0046] The mass concentration of the coating agent solution is 0.1-5%.

[0047] The glucose test solution consists of:

[0048] Glucose oxidase 5-35 ku / L,

[0049] Peroxidase 3-50 ku / L,

[0050] 4-aminoantipyrine 0.1-15 wt%,

[0051] p-hydroxybenzoic acid or sodium 4-hydroxybenzoate 0.1-15 wt%,

[0052] Buffer in the rest.

[0053] The pH of the buffer is 6.0-8.5, and the buffer is selected from one of 0.01-0.5 mol / L Tris-HCL buffer, 0.03-0.3 mol / L Good's buffer, 0.04-0.4 mol / L HEPES buffer, 0.05-0.4 mol / L borate buffer.

[0054] The fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, polyacrylonitrile fiber; the length of the fiber is controlled to be 1-25 mm.

[0055] The master granule is obtained by diatomite treatment, specifically: mixing the master granule with 0.05-1.0 wt% nanoscale white diatomite suspension at a ratio of 1 g:100-2000 mL, ultrasonic mixing for 1-10 minutes, filtering, and drying.

[0056] The master granule contains superabsorbent resin, and the content of superabsorbent resin is 0.5-10%, and the content of fiber material is 90-99.5%.

[0057] A preparation method of a glucose detection granule for cat, comprising the following steps:

[0058] (1) Glucose oxidase, peroxidase, 4-aminoantipyrine and p-hydroxybenzoic acid or sodium 4-hydroxybenzoate are added into a buffer solution, and stirred to obtain a glucose test solution;

[0059] (2) The coating agent is added into deionized water, and stirred to obtain a coating agent solution;

[0060] (3) The glucose test solution and the mother granule are mixed at a weight ratio of 1:5-2:1, and left to stand for 1-15 min, and then dried at one time at a temperature of 35-65 °C until the water content is below 10% to obtain primary reaction granules;

[0061] (4) The coating agent solution is uniformly sprayed on the surface of the primary reaction granules, and then dried at two times at a temperature of 35-65 °C until the water content is below 10% to obtain the glucose detection granules for cats. The coating agent solution is uniformly sprayed on the surface of the primary reaction granules by placing the primary reaction granules on a grid conveyor belt, and then spraying the coating agent solution on the upper and lower surfaces.

[0062] A glucose indicating cat litter is obtained by mixing the glucose detection granules for cats and the cat litter at a weight ratio of 1:100-500. After testing the cat urine containing glucose, a very good color index effect is also obtained.

[0063] Example 1

[0064] A glucose detection granule for cats is obtained by using a mother granule of a fibrous material as a carrier, mixing the glucose test solution and the mother granule at a weight ratio of 1:1.5, leaving to stand for 5 min, then drying at one time at 55 °C ± 2 °C until the water content is below 10%, and then uniformly spraying the coating agent solution on the surface and drying at two times at 55 °C ± 2 °C until the water content is below 10%.

[0065] The glucose test solution comprises:

[0066] glucose oxidase 20 ku / L,

[0067] peroxidase 20 ku / L,

[0068] 4-aminoantipyrine 0.5 wt% (indicator),

[0069] sodium 4-hydroxybenzoate 1 wt% (promoter),

[0070] the buffer solution is selected as a borate buffer solution with a pH of 7.5 and a concentration of 0.2 mol / L.

[0071] The coating agent solution is a sodium carboxymethyl cellulose solution with a mass concentration of 1%.

[0072] The mother granule of the fibrous material is obtained by hot pressing 5-20 mm cotton fibers.

[0073] Glucose indicating litter: glucose detecting granules for cats mixed with bentonite cat litter at a weight ratio of 1:300.

[0074]

[0075] The prepared glucose indicating litter detects glucose after color development as shown in the attached Figure 1 .

[0076] Example 2

[0077] A glucose detecting granule for cats, with a master granule of fibrous material as a carrier, glucose test solution mixed with the master granule at a weight ratio of 1:1, and then placed for 5 min, and then dried at 45℃±2℃ for the first time until the water content is below 10%, and then sprayed with a coating agent solution on the surface and dried at 45℃±2℃ for the second time until the water content is below 10%.

[0078] The glucose test solution is composed of:

[0079] Glucose oxidase 15 ku / L,

[0080] Peroxidase 25 ku / L,

[0081] 4-aminoantipyrine 0.8 wt% (indicator),

[0082] p-hydroxybenzoic acid 2 wt% (promoter),

[0083] Buffer pH 6.5, the rest of the HEPES buffer 0.1 mol / L.

[0084] The coating agent solution is a sodium carboxymethyl cellulose solution with a mass concentration of 1%.

[0085] The master granule of fibrous material is hot-pressed from 3-12 mm paper fibers.

[0086] Glucose indicating litter: glucose detecting granules for cats mixed with plant cat litter (such as paper cat litter, bamboo cat litter, etc.) at a weight ratio of 1:500.

[0087]

[0088] The prepared glucose indicating litter detects glucose after color development as shown in the attached Figure 2 .

[0089] Example 3

[0090] A glucose detection granule for cats, taking a master granule of fiber material as a carrier, mixing a glucose test solution with the master granule at a weight ratio of 2:1, standing for 5 min, then first drying at 50℃±2℃ until the water content is below 10%, then uniformly spraying a coating agent solution on the surface and second drying at 50℃±2℃ until the water content is below 10% to obtain.

[0091] The glucose test solution is composed of:

[0092] Glucose oxidase 35 ku / L,

[0093] Peroxidase 15 ku / L,

[0094] 4-aminoantipyrine 1 wt% (indicator),

[0095] p-hydroxybenzoic acid 2 wt% (promoter),

[0096] Buffer pH 7.5, the rest of Tris-HCL buffer solution is 0.07 mol / L.

[0097] The mass concentration of the coating agent solution is 1%, and the coating agent is composed of sodium carboxymethyl cellulose and polyvinyl alcohol at a mass ratio of 1:1.

[0098] The master granule of fiber material is hot-pressed from 3-12 mm paper fibers.

[0099] Glucose indicating cat litter: mixed by a glucose detection granule for cats and silica gel cat litter at a weight ratio of 1:100.

[0100]

[0101] The glucose indicating cat litter is detected after the glucose color development Figure 3 .

[0102] Example 4

[0103] A glucose detection granule for cats, taking a master granule of fiber material as a carrier, mixing a glucose test solution with the master granule at a weight ratio of 1:1.5, standing for 5 min, then first drying at 55℃±2℃ until the water content is below 10%, then uniformly spraying a coating agent solution on the surface and second drying at 55℃±2℃ until the water content is below 10% to obtain.

[0104] The glucose test solution is composed of:

[0105] Glucose oxidase 25 ku / L,

[0106] Peroxidase 25 ku / L,

[0107] 4-aminoantipyrine 2 wt% (indicator),

[0108] 4-hydroxybenzoic acid sodium 1 wt% (promoter),

[0109] Buffer pH 6.8, the balance of 0.1 mol / L borate buffer.

[0110] The wrapping agent solution is a sodium carboxymethyl cellulose solution with a mass concentration of 0.8%.

[0111] The master batch of the fibrous material is hot-pressed from 5-20 mm cotton fibers.

[0112] The master batch is treated with diatomite, specifically: the master batch is mixed with a 0.5 wt% nanoscale white diatomite suspension at a ratio of 1 g:500 mL, ultrasonic mixing for 3 minutes, filtration, and drying to a water content of less than 10%.

[0113]

[0114] Example 5

[0115] A glucose detection granule for cats, with a master batch of fibrous material as the carrier, glucose test solution is mixed with the master batch at a weight ratio of 1:1.5, and then placed for 5 min, followed by first drying at 55℃±2℃ to a water content of less than 10%, and then uniformly spraying a wrapping agent solution on the surface and second drying at 55℃±2℃ to a water content of less than 10%.

[0116] The glucose test solution consists of:

[0117] Glucose oxidase 25 ku / L,

[0118] Peroxidase 25 ku / L,

[0119] 4-aminoantipyrine 2 wt% (indicator),

[0120] 4-hydroxybenzoic acid sodium 1 wt% (promoter),

[0121] Buffer pH 8.5, the balance of 0.07 mol / L borate buffer.

[0122] The wrapping agent solution is a sodium carboxymethyl cellulose solution with a mass concentration of 1%.

[0123] The master batch of the fibrous material is hot-pressed from 5-25 mm cotton fibers and a superabsorbent resin (commercially available, such as Japan Sumitomo Precision SA60S).

[0124]

[0125] Example 6

[0126] The difference between this embodiment and embodiment 5 is that the master batch is treated by diatomite, specifically, the master batch is mixed with 0.5wt% nano white diatomite suspension in the ratio of 1g:500mL, ultrasonic mixing for 3 minutes, filtering, drying to the water content below 10%. The others are the same as embodiment 5.

[0127]

[0128] Comparative example 1

[0129] The difference between this embodiment and embodiment 4 is that the master batch is not treated by diatomite, the others are the same as embodiment 4. The obtained glucose detection particles are:

[0130]

[0131] The diatomite treated particle group (comparative example 1) and the diatomite treated particle group (embodiment 4) of different batches are mixed with 4.5kg of actual cat litter, 15ml of 5.5mmol / L glucose standard test solution is poured into the test. The colored particles are selected, and the color and the time of discoloration are observed.

[0132]

[0133] It can be seen that after the diatomite treatment, the display time is obviously increased.

[0134] The above described embodiments are only a preferred scheme of the present application, and do not limit the present application in any form, and other variants and modifications are also possible without exceeding the technical scheme recorded in the claims.

Claims

1. A glucose test particle for cats, characterized in that, The glucose test solution is mixed with the master batch of fiber material at a weight ratio of 1:5-2:1, and then left to stand for 1-15 min, and then dried at 35-65℃ to a moisture content of less than 10% for the first time, and then sprayed with a coating agent solution and dried at 35-65℃ for the second time to a moisture content of less than 10% to obtain the product; the master batch of fiber material is hot-pressed; The coating agent is selected from one or more of gelatin, gum arabic, sodium carboxymethyl cellulose, polyvinyl alcohol, and hydroxyethyl cellulose; the mass concentration of the coating agent solution is 0.1-5%; The fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, and polyacrylonitrile fiber; the length of the fiber is controlled to be 1-25 mm; The glucose test solution comprises: Glucose oxidase 5-35 ku / L, Peroxidase 3-50 ku / L, 4-Aminoantipyrine 0.1-1.5 wt%, p-Hydroxybenzoic acid or sodium 4-hydroxybenzoate 0.1-1.5 wt%, Buffer in the rest.

2. The glucose detection granule for cats according to claim 1, characterized by The pH of the buffer is 6.0-8.5, and the buffer is selected from one of 0.01-0.5 mol / L Tris-HCL buffer, 0.03-0.3 mol / L Good's buffer, 0.04-0.4 mol / L HEPES buffer, and 0.05-0.4 mol / L borate buffer.

3. The glucose detection granule for cats according to claim 1, characterized by The master batch is obtained by diatomite treatment, specifically: the master batch is mixed with a 0.05-1.0 wt% nanoscale white diatomite suspension at a ratio of 1 g:100-2000 mL, ultrasonically mixed for 1-10 min, filtered, and dried.

4. The glucose detection granule for cats according to claim 1, characterized by The master batch contains a superabsorbent resin, and the content of the superabsorbent resin is 0.5-10%, and the content of the fiber material is 90-99.5%.

5. A method of preparing a glucose test particle for cats as claimed in claim 1, characterized in that, The method comprises the following steps: (1) Glucose oxidase, peroxidase, 4-aminoantipyrine, and p-hydroxybenzoic acid or sodium 4-hydroxybenzoate are added to a buffer solution, and stirred to obtain a glucose test solution; (2) The coating agent is added to deionized water, and stirred to obtain a coating agent solution; (3) The glucose test solution is mixed with the master batch of fiber material at a weight ratio of 1:5-2:1, and then left to stand for 1-15 min, and then dried at 35-65℃ to a moisture content of less than 10% for the first time to obtain primary reaction particles; (4) The surface of the primary reaction particles is sprayed with the coating agent solution, and then dried at 35-65℃ for the second time to a moisture content of less than 10% to obtain the glucose detection particles for cats.

6. A glucose indicating cat litter, characterized in that, The glucose detection particles for cats of claim 1 are mixed with cat litter at a weight ratio of 1:100-500.

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