Lactose detection device and detection method

Abstract

The present invention relates to the technical field of lactose detection, and provides a lactose detection device and a detection method. The lactose detection device comprises: a heating box; a tray, which is slidably arranged below the heating box, and the tray is provided with a lactose detection test strip; a first syringe, which is arranged through the heating box and is located above the sliding path of the lactose detection test strip, and the first syringe is suitable for holding a mixture of milk sample liquid and glucose oxidase, and is used to inject the heated mixture onto the lactose detection test strip; a second syringe, which is arranged on the side of the heating box and is located above the sliding path of the lactose detection test strip, and the second syringe is suitable for holding a buffer solution, and is used to inject the buffer solution onto the lactose detection test strip after the first syringe completes the injection. The present invention can realize rapid, sensitive and specific detection of milk lactose, and the detection device has the characteristics of simple structure, easy to carry, high detection accuracy and efficiency, etc.

Description

Technical Field

[0001] The present invention relates to the technical field of lactose detection, and in particular to a lactose detection device and a detection method. Background Art

[0002] Milk is rich in nutrients, but the human body is often intolerant to the lactose in milk due to the lack of lactase in the body. If milk containing lactose is consumed, it will cause symptoms such as abdominal pain and diarrhea, so the determination of lactose in milk is very important.

[0003] In the related art, lactose detection devices have the problems of complex structure, inconvenience in carrying, high cost, low detection accuracy and efficiency, and cannot meet the needs of on-site detection. Summary of the invention

[0004] The present invention provides a lactose detection device and a detection method, which can realize rapid, sensitive and specific detection of milk lactose. The detection device has the characteristics of simple structure, small size, easy to carry, high detection accuracy and efficiency, etc., can meet the needs of on-site detection, and is particularly suitable for areas with backward experimental conditions.

[0005] The present invention provides a lactose detection device, comprising:

[0006] Heating box;

[0007] A tray is slidably disposed below the heating box, and the tray is provided with a lactose test strip;

[0008] A first syringe is disposed through the heating box and is located above the sliding path of the lactose test paper, the first syringe is suitable for containing a mixture of milk sample liquid and glucose oxidase, and is used to inject the heated mixture onto the lactose test paper;

[0009] The second syringe is arranged on the side of the heating box and located above the sliding path of the lactose test strip. The second syringe is suitable for containing a buffer solution and is used to inject the buffer solution into the lactose test strip after the first syringe completes the injection.

[0010] According to a lactose detection device provided by the present invention, the tray is provided with two rows of lactose detection test strips, each row of the lactose detection test strips includes a plurality of lactose detection test strips arranged along the sliding direction of the tray, and two of the first syringes and two of the second syringes are respectively provided.

[0011] According to a lactose detection device provided by the present invention, the first syringe and the second syringe are respectively connected to a driving mechanism, and the driving mechanism comprises:

[0012] A fixing plate connected between the syringe barrels of the two corresponding syringes;

[0013] A driving plate, connected between the piston rods of the two corresponding syringes and provided with a receiving groove;

[0014] A screw rod vertically penetrates the middle portion of the driving plate and is connected to the middle portion of the fixing plate;

[0015] The knob is arranged in the receiving groove and is threadedly connected with the screw rod to control the up and down movement of the driving plate.

[0016] A lactose detection device provided according to the present invention further includes a base, the heating box is arranged on the base, the base is provided with a slide groove, and the tray is slidably arranged in the slide groove.

[0017] According to a lactose detection device provided by the present invention, an annular pressing plate is provided on the base, and the annular pressing plate is mounted on the top of the slide groove to construct a guide channel for sliding guidance of the tray.

[0018] According to a lactose detection device provided by the present invention, the tray is provided with an indicator label for indicating the serial numbers of the lactose detection test strips injected by the first syringe and the second syringe; and a positioning ring is provided on the outer side of the annular pressure plate for positioning the serial numbers of the lactose detection test strips.

[0019] A lactose detection device provided according to the present invention also includes a gantry, which is mounted on the annular pressure plate along the vertical direction of the sliding of the tray and connected to the base, and the top of the gantry is provided with a second positioning hole adapted to the needle of the second syringe.

[0020] According to a lactose detection device provided by the present invention, the top side surface of the heating box protrudes outward to construct a support frame, and the second syringe is arranged through the support frame.

[0021] According to a lactose detection device provided by the present invention, the heating box is provided with a water injection port for injecting hot water into the heating box; and / or,

[0022] A first positioning hole matched with the needle of the first syringe is provided at the bottom of the heating box, and a sealing ring is provided at the connection between the needle of the first syringe and the first positioning hole.

[0023] The present invention also provides a detection method according to the above-mentioned lactose detection device, comprising:

[0024] Putting the milk sample liquid and glucose oxidase into the first syringe and mixing them to obtain a mixed liquid;

[0025] heating the mixed liquid by the heating box;

[0026] Injecting the heated mixed solution onto the lactose test paper through the first syringe;

[0027] After the first syringe completes the injection, the tray is slid and the buffer solution is injected onto the lactose test strip through the second syringe.

[0028] The lactose detection device and detection method provided by the present invention realize the decomposition of the original glucose in the milk sample by placing glucose oxidase in the first syringe, and fully decompose and dissipate the H2O2 obtained by decomposing the glucose by heating in a heating box, so as to pre-treat the milk sample, and effectively improve the detection accuracy; by sliding the tray, the lactose detection test paper can be moved to the bottom of the first syringe, and the pre-treated mixed solution is injected onto the lactose detection test paper by the first syringe, after the injection is completed, the tray is slid to move the lactose detection test paper to the bottom of the second syringe, and the buffer solution is injected onto the lactose detection test paper by the second syringe for color development reaction, if there is lactose in the milk, the lactose detection test paper shows color, otherwise it is colorless. Therefore, the present invention can directly observe the color of the lactose detection test paper by naked eyes, and can judge whether there is lactose in the milk, and realize the rapid, sensitive and specific detection of milk lactose; and the detection device integrates the milk sample pretreatment step with the detection reaction, so as to realize the portability of the detection, and has the characteristics of simple structure, small volume, easy to carry, high detection accuracy and efficiency, and can meet the needs of on-site detection.

[0029] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the present invention or related technologies, the drawings required for use in the embodiments or related technical descriptions are briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 It is a structural schematic diagram of the lactose detection device provided by the present invention;

[0032] Figure 2 is a side view of the lactose detection device provided by the present invention;

[0033] Figure 3 is a top view of the lactose detection device provided by the present invention;

[0034] Figure 4 yes Figure 3 AA section view;

[0035] Figure 5 It is a structural schematic diagram of a tray provided by the present invention;

[0036] Figure 6 is a sampling state diagram of the first syringe provided by the present invention;

[0037] Reference numerals:

[0038] 1: Heating box; 2: Tray; 3: First syringe; 4: Second syringe;

[0039] 5: placement slot; 6: fixing plate; 7: driving plate; 8: screw rod; 9: knob;

[0040] 10: accommodating groove; 11: lug; 12: first slot; 13: flange; 14: base;

[0041] 15: slide groove; 16: annular pressure plate; 161: positioning ring; 17: first indication label;

[0042] 18: second indicator label; 19: tray body; 20: support handle; 21: gantry;

[0043] 22: second positioning hole; 23: crossbeam; 24: supporting leg; 25: boss; 26: supporting frame;

[0044] 27: water injection port; 28: first positioning hole; 29: sealing ring; 30: box body;

[0045] 31: box cover; 32: milk sample liquid box. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] In the description of the embodiments of the present invention, it should be noted that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the embodiments of the present invention. In addition, the terms "first", "second", etc., are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0048] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0049] In the embodiments of the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0051] Combine the following Figure 1-Figure 6 The lactose detection device and detection method of the present invention are described.

[0052] According to an embodiment of the present invention, Figure 1-Figure 6 As shown, the lactose detection device provided by the present invention mainly includes: a heating box 1, a tray 2, a first syringe 3 and a second syringe 4. Among them, the heating box 1 is mainly used to heat the first syringe 3; the tray 2 is slidably arranged under the heating box 1, and the tray 2 is provided with a lactose detection test paper, and the lactose detection test paper can detect lactose. When using the tray 2, the lactose detection test paper can be driven to move to the bottom of the first syringe 3 and the second syringe 4 by sliding the tray 2, so as to realize the rapid detection of the milk sample liquid, which is very convenient to use.

[0053] The first syringe 3 is suitable for containing a mixture of milk sample liquid and glucose oxidase. The first syringe 3 is arranged on the heating box 1, and the mixture can be heated by the heating box 1. The first syringe 3 is located above the sliding path of the lactose test paper. Specifically, the syringe of the first syringe 3 is fixed to the top of the heating box 1, and at least part of the syringe of the first syringe 3 is located in the heating box 1. The piston rod of the first syringe 3 is located above the outside of the heating box 1, and the needle of the first syringe 3 extends through the bottom of the heating box 1. When dropping the sample, slide the tray 2 to move the lactose test paper to the bottom of the first syringe 3. By pushing the piston rod of the first syringe 3, the heated mixture in the first syringe 3 can be injected onto the lactose test paper.

[0054] It should be noted that, due to the high glucose content in the milk sample, it will cause serious interference to the detection result of lactose and reduce the detection accuracy. Therefore, the present invention can preliminarily realize the decomposition of the original glucose in the milk sample by placing glucose oxidase in the first syringe 3. After the decomposition, hydrogen peroxide will be produced, which will affect the detection result and reduce the detection accuracy. Therefore, the present invention further heats the heating box 1 to realize the decomposition of hydrogen peroxide, thereby effectively improving the detection accuracy and realizing sensitive and specific detection of milk lactose.

[0055] The second syringe 4 is suitable for containing a buffer solution, which can provide a stable reaction environment and thus improve the detection accuracy. The second syringe 4 is arranged on the side of the heating box 1, and the second syringe 4 is located above the sliding path of the lactose test paper. Specifically, the second syringe 4 is arranged side by side with the first syringe 3, and the syringe barrel of the second syringe 4 is fixed to the top side of the heating box 1, and the piston rod of the second syringe 4 is located above the outside of the heating box 1. When the first syringe 3 completes the injection, the buffer solution in the second syringe 4 can be injected onto the lactose test paper for a color reaction by pushing the piston rod of the second syringe 4.

[0056] The lactose detection device provided in the embodiment of the present invention realizes the decomposition of the original glucose in the milk sample by placing glucose oxidase in the first syringe 3, and fully decomposes and dissipates the H2O2 obtained by the decomposition of glucose by heating through the heating box 1, so as to pre-treat the milk sample, which can effectively improve the detection accuracy; by sliding the tray 2, the lactose detection test paper can be moved to the bottom of the first syringe 3, and the pretreated mixed solution is injected onto the lactose detection test paper through the first syringe 3. After the injection is completed, the tray 2 is slid to move the lactose detection test paper to the bottom of the second syringe 4, and the buffer solution is injected into the lactose detection test paper through the second syringe 4 for a color reaction. If lactose exists in the milk, the lactose detection test paper will show color, otherwise it will be colorless. Therefore, the present invention can directly judge whether lactose is present in milk by observing the color of the lactose test paper with the naked eye, thereby realizing rapid, sensitive and specific detection of milk lactose; and the detection device integrates the milk sample liquid pretreatment step with the detection reaction, thereby realizing the portability of the detection, and has the characteristics of simple structure, small size, easy to carry, high detection accuracy and efficiency, etc., which can meet the needs of on-site detection.

[0057] According to one embodiment of the present invention, Figure 1 and Figure 5 As shown, the tray 2 is provided with two rows of lactose test strips, each row of lactose test strips includes a plurality of lactose test strips, and the plurality of lactose test strips are arranged along the sliding direction of the tray 2. Accordingly, two first syringes 3 and two second syringes 4 are respectively provided, and each first syringe 3 and each second syringe 4 can inject the plurality of lactose test strips in each row. By providing a plurality of lactose test strips and corresponding syringes, the embodiment of the present invention can detect a plurality of milk samples at the same time, thereby effectively improving the detection accuracy and efficiency of the device.

[0058] According to an embodiment of the present invention, a placement slot 5 adapted to the lactose test strip is provided on the tray 2 for placing the lactose test strip to achieve positioning and installation of the lactose test strip.

[0059] According to one embodiment of the present invention, Figure 1-Figure 4 As shown, the first syringe 3 and the second syringe 4 are respectively connected with a driving mechanism, and the driving mechanism is arranged above the heating box 1 for easy operation. Specifically, the driving mechanism is divided into a first driving mechanism and a second driving mechanism, the first driving mechanism is connected to the first syringe 3 to drive the first syringe 3 to inject, and the second driving mechanism is connected to the second syringe 4 to drive the second syringe 4 to inject, and the first driving mechanism and the second driving mechanism have the same structure.

[0060] The driving mechanism mainly includes: a fixed plate 6, a driving plate 7, a screw 8 and a knob 9. The fixed plate 6 is connected between the syringes of the two corresponding syringes, that is, the two ends of the fixed plate 6 of the first driving mechanism are respectively connected to the syringes of the two first syringes 3, and the two ends of the fixed plate 6 of the second driving mechanism are respectively connected to the syringes of the two second syringes 4; the driving plate 7 is arranged in parallel with the fixed plate 6, the driving plate 7 is located above the fixed plate 6, and the driving plate 7 is connected between the piston rods of the two corresponding syringes, that is, the two ends of the driving plate 7 of the first driving mechanism are respectively connected to the piston rods of the two first syringes 3, and the two ends of the driving plate 7 of the second driving mechanism are respectively connected to the piston rods of the two second syringes 4; and the driving plate 7 is provided with a receiving groove 10.

[0061] The screw 8 vertically penetrates the middle of the driving plate 7 and is connected to the middle of the fixing plate 6, so that the forces on the two rows of syringes are uniform, thus ensuring that the injection drop amounts of the two rows are consistent, thereby improving the parallelism of the detection.

[0062] The knob 9 is disposed in the receiving groove 10 , and a portion of the knob 9 is exposed to the outside, so that the user can turn the knob 9 , and the knob 9 is threadedly connected to the screw rod 8 .

[0063] The working principle of the driving mechanism of the embodiment of the present invention is described below, which generally includes: when the knob 9 is turned, the knob 9 drives the driving plate 7 to move up and down, and the driving plate 7 drives the piston rod of the syringe to move up and down to achieve the injection drop operation. In other words, the height of the syringe piston rod can be adjusted up and down by turning the knob 9, thereby controlling the injection amount of the syringe. Therefore, the driving mechanism of the present invention has the characteristics of simple structure, low cost, easy use and particularly labor-saving.

[0064] In the related art, the driving source generally adopts a motor, which will cause the overall weight to be too large, inconvenient to carry, and increase the cost. In addition, an external power supply is required when in use, and when there is no power supply at the detection site, it cannot even be used, that is, it cannot better adapt to on-site detection. The driving mechanism of the embodiment of the present invention avoids the use of a motor, which can reduce the cost, reduce the overall weight, and facilitate carrying to the on-site detection.

[0065] According to one embodiment of the present invention, Figure 1 As shown, the top of the syringe barrel of the first syringe 3 and the second syringe 4 are both provided with a lug 11, and the front and rear ends of the fixing plate 6 are respectively provided with a first card groove 12 adapted to the lug 11. During assembly, the lug 11 can be inserted into the first card groove 12 to realize the assembly connection between the syringe and the corresponding fixing plate 6.

[0066] Furthermore, the first slot 12 runs through the left and right sides of the fixing plate 6. With such a design, the lug 11 of the syringe can be inserted into or removed from the first slot 12 by simply rotating the syringe barrel, which makes installation quick and easy.

[0067] According to one embodiment of the present invention, the top ends of the piston rods of the first syringe 3 and the second syringe 4 are both provided with a flange 13, and the front and rear ends of the driving plate 7 are respectively provided with second card slots adapted to the flange 13. During assembly, the flange 13 can be inserted into the second card slot to realize the assembly connection between the syringe and the corresponding driving plate 7.

[0068] According to one embodiment of the present invention, Figure 1 As shown, the lactose detection device further includes a base 14, the heating box 1 is disposed on the base 14, the base 14 is provided with a slide groove 15, and the tray 2 can be slidably disposed in the slide groove 15. By providing the base 14, the embodiment of the present invention can integrate all components on the base 14, thereby improving the portability of the entire device.

[0069] According to one embodiment of the present invention, an annular pressure plate 16 is provided on the base 14, and the annular pressure plate 16 is mounted on the top of the slide groove 15. The gap between the annular pressure plate 16 and the slide groove 15 constructs a guide channel, and the tray 2 can slide in the guide channel, that is, the sliding guidance of the tray 2 can be achieved by setting the annular pressure plate 16.

[0070] According to one embodiment of the present invention, the annular pressing plate 16 is arranged side by side on the right side of the heating box 1 and below the second syringe 4 .

[0071] According to one embodiment of the present invention, Figure 1 and Figure 5 As shown, the tray 2 is provided with an indicator label for indicating the serial number of the lactose test paper injected by the first syringe 3 and the second syringe 4. Specifically, the tray 2 is provided with a plurality of groups of first indicator labels 17 and second indicator labels 18 arranged along the sliding direction, the first indicator label 17 indicates the serial number of the test paper injected by the first syringe 3, and the second indicator label 18 indicates the serial number of the test paper injected by the second syringe 4; a positioning ring 161 is provided on the right side of the annular pressure plate 16 for positioning the test paper serial number to realize sample addition indication.

[0072] According to one embodiment of the present invention, the tray 2 includes a tray body 19 and a handle 20, two rows of lactose test strips are arranged on the tray body 19, the handle 20 is connected to the middle of the rear end of the tray body 19, and multiple groups of first indicator labels 17 and second indicator labels 18 are arranged on the tray body 19 and the handle 20 and are located between the two rows of lactose test strips; the tray 2 can be slid by moving the handle 20.

[0073] According to one embodiment of the present invention, Figure 1As shown, the lactose detection device also includes a gantry 21, which is mounted on the annular pressing plate 16 along the vertical direction (front-back direction) of the sliding of the tray 2 and is connected to the front and back sides of the base 14. The gantry 21 is arranged below the second syringe 4, and the top of the gantry 21 is provided with a second positioning hole 22 adapted to the needle of the second syringe 4. The needle of the second syringe 4 passes through the second positioning hole 22, which is convenient for dripping buffer solution to the lactose detection test paper below. The present invention can press the annular pressing plate 16 against the base 14 to fix the annular pressing plate 16 through the gantry 21, and can also realize the positioning of the second syringe 4.

[0074] According to one embodiment of the present invention, the gantry 21 includes: a crossbeam 23 and a leg 24, the crossbeam 23 is provided with a second positioning hole 22 adapted to the needle of the second syringe 4; the front and rear ends of the crossbeam 23 are respectively provided with legs 24, the inner sides of the bottoms of the two legs 24 are provided with pressing grooves, and the two legs 24 can press the annular pressure plate 16 onto the base 14 through the pressing grooves, and a boss 25 is formed on the inner sides of the two legs 24, the boss 25 is arranged opposite to the pressing groove, and a clamping portion is formed at the bottom of the boss 25, and a clamping groove is provided on the front and rear side edges of the base 14, and the clamping portion can be clamped into the clamping groove, thereby realizing the detachable assembly of the gantry 21, the annular pressure plate 16 and the base 14.

[0075] According to one embodiment of the present invention, the top right side of the heating box 1 protrudes outward to form a support frame 26, and the syringe of the second syringe 4 is arranged on the support frame 26. The embodiment of the present invention facilitates the installation of the second syringe 4 by providing the support frame 26.

[0076] According to one embodiment of the present invention, Figure 1 and Figure 3 As shown, a water injection port 27 is provided on the top of the heating box 1. Of course, the water injection port 27 can also be located at other positions of the heating box 1 for injecting hot water of a required temperature into the heating box 1 to perform water bath heating on the first injector 3.

[0077] According to one embodiment of the present invention, Figure 4 As shown, a first positioning hole 28 adapted to the needle of the first syringe 3 is provided at the bottom of the heating box 1, and a sealing ring 29 is provided at the connection between the needle of the first syringe 3 and the first positioning hole 28 to ensure sealing and prevent water leakage.

[0078] According to one embodiment of the present invention, the heating box 1 includes: a box body 30 and a box cover 31, the box cover 31 is detachably connected to the top of the box body 30, and the box cover 31 partially blocks the top of the box body 30 to form a water inlet 27 on one side of the box body 30, and a support frame 26 is provided on the right side of the box cover 31; the front and rear sides of the bottom of the box body 30 are respectively provided with raised parts, and the front and rear side edges of the base 14 are provided with snap-in grooves matched with the raised parts, so as to realize the detachable assembly of the heating box 1 and the base 14.

[0079] According to one embodiment of the present invention, the lactose detection device of the present invention is made by 3D printing, which does not require expensive molds, can be produced in small batches, and has low cost. Specifically, the drive mechanism can be printed with polylactic acid material, wherein the screw 8 can also be made of stainless steel; the heating box 1 can be printed with polycarbonate material, which can withstand temperatures from -45°C to 135°C; the base 14, tray 2, annular pressure plate 16, gantry 21 and other components can all be printed with polylactic acid material.

[0080] The working principle of the lactose detection device provided by the present invention is described below, which generally includes:

[0081] (1) Pretreatment of milk sample: Remove the first syringe 3, draw a certain volume of the milk sample to be tested into the milk sample box 32 and mix it with the glucose oxidase in the first syringe 3, as shown in FIG. Figure 6 Then the first syringe 3 is returned to its original position and incubated at room temperature for a certain period of time. Hot water of a certain temperature is injected into the heating box 1 through the water injection port 27, and the milk sample liquid is pretreated in a water bath for a certain period of time.

[0082] (2) Test strip color reaction: Slide the tray 2 to move the lactose test strip to the bottom of the first syringe 3, rotate the knob 9 of the first drive mechanism, drip the pre-treated test mixture in the first syringe 3 onto the lactose test strip below, then slide the tray 2 to move the lactose test strip to the bottom of the second syringe 4, rotate the knob 9 of the second drive mechanism, drip the buffer solution in the second syringe 4 onto the lactose test strip below for color reaction. Use the tray 2 to move multiple lactose test strips and complete the test one by one.

[0083] The present invention also provides a detection method of the lactose detection device according to the above embodiment, which mainly comprises:

[0084] Put the milk sample liquid and glucose oxidase into the first syringe 3 and mix them to obtain a mixed solution;

[0085] Heating the mixed liquid by means of a heating box 1;

[0086] Injecting the heated mixed solution onto the lactose test paper through the first syringe 3;

[0087] After the first syringe 3 completes the injection, the tray 2 is slid, and the buffer solution is injected onto the lactose test paper through the second syringe 4 for color reaction. If lactose exists in the milk, the lactose test paper displays color, otherwise it is colorless. Therefore, the present invention can directly determine whether lactose exists in the milk by observing the color of the lactose test paper with naked eyes, and realize the rapid detection of milk lactose, and the whole detection process takes less than 25 minutes.

[0088] The detection method of the present invention is further described below in conjunction with a specific embodiment.

[0089] (1) Preparation of lactose test strips

[0090] Use PBS to prepare a solution containing 100 U / mL β-galactosidase, 100 U / mL glucose oxidase, 12 μg / mL nanozyme with peroxidase-like activity, and 100 μM TMB. Soak the test paper for 10 seconds, take it out and dry it at room temperature, and use a hole punch to cut it to obtain the required lactose detection test paper.

[0091] (2) Milk sample pretreatment

[0092] The first syringe 3 is pre-loaded with 0.9 mL of glucose oxidase (20 U / mL). During the test, 0.1 mL of the milk sample to be tested is sucked into the first syringe 3, and the first syringe 3 is shaken to mix the sample. The sample is allowed to stand at room temperature for 5 minutes, and then 100°C hot water is injected into the heating box 1. The first syringe 3 is heated for 5 minutes.

[0093] (3) Lactose detection

[0094] By rotating the knob 9 of the first driving mechanism, the first syringe 3 is used to drip the pre-treated milk sample onto the lactose test paper and incubate for 4 minutes. The amount of dripping is appropriate to soak the test paper. Then, by rotating the knob 9 of the second driving mechanism, a drop of NaAc-HAc buffer (pH 3.6) is dripped from the second syringe 4 and allowed to stand for 5 minutes. The color of the test paper is observed with the naked eye. If lactose is present in the milk, the test paper is blue-green, otherwise it is colorless.

[0095] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A detection method of a lactose detection device, characterized in that: The device comprises: Heating box; A tray is slidably disposed below the heating box, and the tray is provided with a lactose test strip; A first syringe is disposed through the heating box and is located above the sliding path of the lactose test paper, the first syringe is suitable for containing a mixture of milk sample liquid and glucose oxidase, and is used to inject the heated mixture onto the lactose test paper; a second syringe, disposed on a side of the heating box and located above the sliding path of the lactose test strip, the second syringe being suitable for containing a buffer solution, and being used to inject the buffer solution onto the lactose test strip after the first syringe completes the injection; The method comprises: Putting the milk sample liquid and glucose oxidase into the first syringe and mixing them to obtain a mixed liquid; heating the mixed liquid by the heating box; Injecting the heated mixed solution onto the lactose test paper through the first syringe; After the first syringe completes the injection, the tray is slid and the buffer solution is injected onto the lactose test strip through the second syringe.

2. The detection method of the lactose detection device according to claim 1, characterized in that: The tray is provided with two rows of the lactose test strips, each row of the lactose test strips includes a plurality of the lactose test strips arranged along the sliding direction of the tray, and two of the first syringes and two of the second syringes are respectively provided.

3. The detection method of the lactose detection device according to claim 2, characterized in that: The first syringe and the second syringe are respectively connected to a driving mechanism, and the driving mechanism comprises: A fixing plate connected between the syringe barrels of the two corresponding syringes; A driving plate, connected between the piston rods of the two corresponding syringes and provided with a receiving groove; A screw rod vertically penetrates the middle portion of the driving plate and is connected to the middle portion of the fixing plate; The knob is arranged in the receiving groove and is threadedly connected with the screw rod to control the up and down movement of the driving plate.

4. The detection method of the lactose detection device according to any one of claims 1 to 3, characterized in that: It also includes a base, the heating box is arranged on the base, the base is provided with a slide groove, and the tray can be slidably arranged in the slide groove.

5. The detection method of the lactose detection device according to claim 4, characterized in that: An annular pressing plate is arranged on the base, and the annular pressing plate is mounted on the top of the slide groove to construct a guide channel for guiding the sliding of the tray.

6. The detection method of the lactose detection device according to claim 5, characterized in that: The tray is provided with an indication label for indicating the serial numbers of the lactose test strips injected by the first syringe and the second syringe; the outer side of the annular pressing plate is provided with a positioning ring for positioning the serial numbers of the lactose test strips.

7. The detection method of the lactose detection device according to claim 5, characterized in that: It also includes a gantry, which is erected on the annular pressure plate along the vertical direction of the sliding of the tray and connected to the base. The top of the gantry is provided with a second positioning hole adapted to the needle of the second syringe.

8. The detection method of the lactose detection device according to any one of claims 1 to 3, characterized in that: The top side of the heating box protrudes outward to form a support frame, and the second syringe is arranged through the support frame.

9. The detection method of the lactose detection device according to claim 8, characterized in that: The heating box is provided with a water injection port for injecting hot water into the heating box; and / or, A first positioning hole matched with the needle of the first syringe is provided at the bottom of the heating box, and a sealing ring is provided at the connection between the needle of the first syringe and the first positioning hole.

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