A sample support device for microscopic observation

Through gear meshing and thread-driven transmission components, the precise adjustment of the sample support tube under the optical microscope lens is achieved, solving the problem of poor adjustment in existing devices, and improving the observation quality and water resource utilization efficiency.

CN114739906BActive Publication Date: 2025-07-11INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Application Number
CN202210381488.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-04-13
Publication Date
2025-07-11
Estimated Expiration
2042-04-13

AI Technical Summary

Technical Problem

Existing microscopic observation sample support devices cannot accurately adjust the horizontal distance between the sample support tube and the optical lens, resulting in poor observation results.

Method used

The gear meshing and thread drive are used to realize the rotation and linear movement of the sample support tube through transmission parts and adjustment parts, and the position and angle of the sample under the optical microscope lens are accurately adjusted.

Benefits of technology

It improves observation quality and observation effect, has high stability and accuracy, can accurately adjust the distance between the sample support tubes between the lenses, and facilitates the reuse of water.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a sample support device for microscopic observation, which relates to the technical field of optical microscope measurement. It includes a housing assembly and a sample support tube. A dish body is arranged inside the housing assembly. A first transmission part is movably sleeved on one side of the sample support tube close to the pipette connection part. The first transmission part is distributed between the side wall of the dish body and the pipette connection part. It further includes a telescopic part, an adjusting part and a metal plate. The metal plate is fixedly connected to the side wall of the dish body. The adjusting part is rotatably connected between the side wall of the dish body and the metal plate. One end of the adjusting part is movably connected to one end of the sample support tube close to the pipette connection part. The other end of the adjusting part is rotatably connected to the side wall of the dish body. A second transmission part is arranged inside the adjusting part. One ends of the first transmission part and the second transmission part are both movably connected to the inner side of the adjusting part, and the other ends of the first transmission part and the second transmission part are both movably connected to the metal plate. The internal components of the first transmission part and the second transmission part are the same.
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Description

Technical Field

[0001] The present invention relates to the technical field of optical microscope measurement, and specifically to a sample support device for microscopic observation. Background Art

[0002] Optical microscopy techniques include optical microscope techniques and optical microscopic specimen preparation techniques. An optical microscope uses visible light as a light source and magnifies the object to be observed through a set of glass lenses including an eyepiece, an objective lens, and a condenser lens to improve the resolution. When observing a sample using an optical microscope, first, the sample needs to be placed in a special culture dish, and then the culture dish containing the sample is placed on the stage. Finally, the position of the culture dish or the sample in the culture dish and the parameters of the optical lenses are adjusted to achieve the purpose of observing the sample.

[0003] Publication No. CN108593549B discloses a sample support device for microscopic observation, including a sample support tube, a dish body, and a rotation adjustment part. It can not only conveniently and quickly prepare samples, eliminating the complex work of trimming the gel block after sample preparation, but also, after sample preparation, the sample support tube can be rotated through the rotation adjustment part according to the needs of the experimenter to conveniently adjust the posture of the sample to the optimal observation state, solving the problem of not having to prepare samples again due to incorrect sample posture. However, after placing the dish body on the stage of the optical microscope, this patent does not show how to precisely adjust the position of the dish body on the stage, that is, it does not show how to precisely adjust the horizontal distance between the sample support tube in the dish body and the optical lens. The setting of the rotation adjustment part can only adjust the rotation angle of the sample support tube alone, and the horizontal distance between the sample support tube and the optical lens cannot be precisely adjusted through the rotation adjustment part. Summary of the Invention

[0004] The purpose of the present invention is to provide a sample support device for microscopic observation to solve the problems raised in the above background art.

[0005] To achieve the above object, the present invention provides the following technical solution: A sample support device for microscopic observation, comprising a housing assembly and a sample support tube. A dish body is arranged inside the housing assembly. The sample support tube is movably connected in through holes opened on both side walls at both ends of the dish body. One end of the sample support tube is provided with a pipette connection part. A first transmission part is movably sleeved on one side of the sample support tube close to the pipette connection part. The first transmission part is distributed between the side wall of the dish body and the pipette connection part. It further includes a telescopic part, an adjusting part and a metal plate. The metal plate is fixedly connected to the side wall of the dish body. The adjusting part is rotatably connected between the side wall of the dish body and the metal plate. One end of the adjusting part is movably connected to one end of the sample support tube close to the pipette connection part, and the other end of the adjusting part is rotatably connected to the side wall of the dish body. A second transmission part is arranged inside the adjusting part. One ends of the first transmission part and the second transmission part are both movably connected to the inner side of the adjusting part, and the other ends of the first transmission part and the second transmission part are both movably connected to the metal plate. The internal components of the first transmission part and the second transmission part are the same.

[0006] As a further solution of the present invention: The first transmission part includes a transmission gear, a magnet ring and a movable sleeve. The transmission gear is fixedly sleeved on the outer wall of the movable sleeve. Both ends of the movable sleeve are designed with openings. A second limiting groove extending along the axial direction is opened on the inner wall of the movable sleeve. A first limiting rib is connected to one end of the outer wall of the sample support tube close to the pipette connection part. The sample support tube is inserted and connected to the second limiting groove on the movable sleeve through the first limiting rib. A magnet ring is connected to one side of the transmission gear close to the metal plate. The magnet ring is adsorbed and connected to the metal plate. A return spring is further connected between the transmission gear and the metal plate. The return spring is movably sleeved on the movable sleeve. The movable sleeve is also movably sleeved in a second sleeving hole opened on the surface of the metal plate. The transmission gear is movably connected to the adjusting part. A lifting block is installed at one end of the movable sleeve passing through the second sleeving hole.

[0007] As a still further solution of the present invention: The adjusting part is designed as a rotating ring. Two end faces of the rotating ring are respectively rotatably connected to the side wall of the dish body and the side wall of the dish body. A plurality of meshing teeth meshing with the transmission gear are evenly arranged on the inner side of the rotating ring. The thickness value of the meshing teeth is the same as the thickness value of the transmission gear. The thickness value of the transmission gear is less than the gap value between the magnet ring and the metal plate.

[0008] As a further solution of the present invention: The telescopic part further includes a threaded tube and a threaded rod. One end of the threaded tube is rotatably connected to the side wall of the dish body, and the end of the threaded tube does not penetrate the side wall of the dish body. The outer wall of the threaded tube is connected with a second limiting rib extending along the axial direction. The movable sleeve in the second transmission part is connected to the threaded tube through the second limiting rib. The threaded rod is threadedly connected to the end of the threaded tube away from the dish body. The other end of the threaded rod is fixedly connected with a fixing plate. One end of the fixing plate away from the threaded rod is provided with a first sleeving hole. One end of the sample support tube close to the pipette connection part is connected with a telescopic part connecting piece. The telescopic part connecting pieces are distributed between the first limiting ribs and the first limiting ribs. The fixing plate is movably sleeved on the telescopic part connecting piece through the first sleeving hole. When the light of the optical microscope irradiates from bottom to top, in order to prevent the lead screw and the fixed rod from blocking the sample support tube, the lead screw and the fixed rod are arranged on both sides below the lead screw and the fixed rod. When it is necessary to inject water into the dish body, open the sealing cover. When the staff uses it, they only need to press the lifting block downward with their fingers to connect the transmission gear in the third transmission part with the meshing teeth on the adjusting part, and then drive the lead screw to rotate by rotating the adjusting part. The rotating lead screw can drive the piston to move towards the direction close to the water permeable hole. The moving piston can squeeze the water in the water storage chamber into the upper dish body. When the water in the dish body reaches a certain height, then use your finger to pull the lifting block to disengage the transmission gear in the third transmission part from the meshing teeth on the adjusting part. When it is necessary to adjust the rotation angle and horizontal position of the sample support tube by rotating the adjusting part, the lead screw will not be driven to rotate, which has the characteristic of being convenient for injecting water into the dish body.

[0009] As a further solution of the present invention: Sealing sleeves are installed in both through holes on the dish body. A first limiting groove is opened on the inner wall of one of the two sealing sleeves close to the pipette connection part. The first limiting rib on the sample support tube is inserted and connected in the first limiting groove.

[0010] As a further solution of the present invention: The water storage chamber is located directly below the dish body. A partition is provided between the dish body and the water storage chamber. One end of the partition is provided with a water permeable hole communicating with the dish body. A sealing cover is installed in the water permeable hole. The above-mentioned is a sample support device for microscopic observation.

[0011] As a further solution of the present invention: it further includes a pressing part, a third transmission part and a piston. The lead screw is installed on the side walls at both ends of the water storage bin. A third limiting rib extending along the axial direction is connected to the side wall of the lead screw near the adjusting part and exposed at one end of the side wall of the water storage bin. The third transmission part is consistent with the internal components of the first transmission part or the second transmission part. The movable sleeve in the third transmission part is connected to the lead screw through the third limiting rib, and the transmission gear in the third transmission part is meshed and connected to the adjusting part. The third transmission part is also installed on the metal plate through the movable sleeve. The piston is threadedly connected to the lead screw, and the side wall of the piston is in close contact with the inner wall of the water storage bin. A fixed rod parallel to the lead screw is also fixedly connected to the inner wall of the water storage bin. The piston is also movably sleeved on the fixed rod.

[0012] As a further solution of the present invention: the first transmission part, the second transmission part and the third transmission part are all distributed between the adjusting part and the metal plate, and the first transmission part, the second transmission part and the third transmission part are symmetrically distributed about the center of the adjusting part.

[0013] As a further solution of the present invention: an annular guide rail is provided on one side of the adjusting part close to the metal plate. A plurality of fixed pins slidably connected to the annular guide rail are installed on the metal plate, and the central position of the metal plate is connected to the side wall of the housing assembly through a fixed column.

[0014] Compared with the prior art, the beneficial effects of the present invention are:

[0015] 1. When the staff drives the sample support tube to rotate around its own axis by pressing the first transmission part to make it meshed and connected with the adjusting part, the staff can also drive the sample support tube to move linearly along its own axis by pressing the second transmission part to make it meshed and connected with the adjusting part. It can not only adjust the body posture of the sample, but also adjust the horizontal position of the sample support tube in the dish or under the lens of the optical microscope. Compared with the method of directly moving the dish, this technical solution has the characteristics of high stability and accuracy through the way of gear meshing and screw-driven feeding. Furthermore, it improves the observation quality and effect, and has the characteristics of strong adjustability and high adjustment accuracy;

[0016] 2. In the initial state, the magnet ring on the transmission gear is adsorbed on the surface of the metal plate against the elastic force of the return spring. At this time, the transmission gear is also not connected to the meshing teeth. Only when it is necessary to drive the sample support tube, the telescopic part or the pressing part to move through the adjusting part, the magnet ring will be separated from the metal plate by pressing the lifting block. After the magnet ring is separated from the metal plate, there will still be an adsorption force between the two. However, due to the certain distance between the two, the adsorption force between the two is less than the elastic force of the return spring. That is to say, the return spring can fix the position of the return spring at the position meshing with the meshing teeth on the adjusting part. When it is necessary to separate the transmission gear from the adjusting part again, only need to pull the lifting block in the reverse direction with fingers. It has the characteristics of convenient operation and easy adjustment of the movement state of the sample support tube, the telescopic part or the pressing part according to needs, and has strong adjustability;

[0017] 3. When it is necessary to inject water into the dish body, open the sealing cover. The staff only needs to press the lifting block downward with fingers during use to connect the transmission gear in the third transmission part with the meshing teeth on the adjusting part, and then drive the lead screw to rotate by rotating the adjusting part. The rotating lead screw can drive the piston to move towards the direction close to the water permeable hole. The moving piston can squeeze the water in the water storage bin into the upper dish body. When the water in the dish body reaches a certain height, then use fingers to pull the lifting block to separate the transmission gear in the third transmission part from the meshing teeth on the adjusting part. It has the characteristic of being convenient to inject water into the dish body. After the sample observation is completed, repeat the above operation again, and the water in the dish body can be drained back into the water storage bin by moving the piston in the reverse direction, thus realizing the function of water reuse, and has the characteristics of energy saving and resource reuse;

[0018] 4. The pressing part can also achieve the purpose of adjusting the water level height in the dish body according to the height of the lens in the dish body by moving the piston, and has strong practicability. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is the overall assembly drawing of a sample support device for microscopic observation according to an embodiment of the present invention.

[0020] Figure 2 It is the sectional view of the housing assembly in the embodiment of the present invention.

[0021] Figure 3 It is the three-dimensional view of the housing assembly in the embodiment of the present invention.

[0022] Figure 4 It is the three-dimensional view of the sample support tube in the embodiment of the present invention.

[0023] Figure 5 It is the three-dimensional view of the first transmission part in the embodiment of the present invention.

[0024] Figure 6 This is a perspective view of the telescopic part in the embodiment of the present invention.

[0025] Figure 7 This is an assembly drawing of the sample support tube and the telescopic part in the embodiment of the present invention.

[0026] Figure 8 This is a schematic structural diagram of the housing assembly, the sample support tube and the pressing part in the embodiment of the present invention.

[0027] Figure 9 This is a perspective view of the pressing part in the embodiment of the present invention.

[0028] Figure 10 This is a perspective view of the adjusting part in the embodiment of the present invention.

[0029] Figure 11 This is an assembly drawing of the adjusting part, the first transmission part, the second transmission part and the third transmission part in the embodiment of the present invention.

[0030] Figure 12 This is a perspective view of the metal plate in the embodiment of the present invention.

[0031] Figure 13 This is an assembly drawing of the adjusting part, the metal plate, the first transmission part, the second transmission part and the third transmission part in the embodiment of the present invention.

[0032] Figure 14 This is a schematic structural diagram of the first transmission part and the metal plate in the embodiment of the present invention.

[0033] Figure 15 In the present invention Figure 3 Partial enlarged view of a.

[0034] In the figure: 1 - housing assembly, 11 - partition plate, 12 - dish body, 13 - water storage bin, 14 - water permeable hole, 15 - sealing cover, 16 - through hole, 17 - sealing sleeve, 171 - first limiting groove, 2 - sample support tube, 21 - pipette connection part, 22 - telescopic part connecting piece, 23 - first transmission part, 231 - transmission gear, 232 - magnet ring, 233 - movable sleeve, 234 - lifting block, 235 - second limiting groove, 236 - return spring, 24 - first limiting rib, 3 - telescopic part, 31 - threaded tube, 311 - second limiting rib, 32 - threaded rod, 33 - second transmission part, 34 - fixing plate, 35 - first sleeve hole, 4 - pressing part, 41 - lead screw, 411 - third limiting rib, 42 - third transmission part, 43 - fixed rod, 44 - piston, 5 - adjusting part, 51 - meshing tooth, 52 - annular guide rail, 6 - metal plate, 61 - second sleeve hole, 62 - fixing pin, 63 - fixing column. Detailed implementation manners

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0036] Please refer to Figures 1 to 14 , this embodiment provides a sample support device for microscopic observation, including a housing assembly 1 and a sample support tube 2. A dish body 12 is arranged inside the housing assembly 1. The sample support tube 2 is movably connected in a through hole 16 opened on the side walls at both ends of the dish body 12. One end of the sample support tube 2 is provided with a pipette connection part 21. A first transmission part 23 is movably sleeved on one side of the sample support tube 2 close to the pipette connection part 21. The first transmission part 23 is distributed between the side wall of the dish body 12 and the pipette connection part 21. It further includes a telescopic part 3, an adjusting part 5 and a metal plate 6. The metal plate 6 is fixedly connected to the side wall of the dish body 12. The adjusting part 5 is rotatably connected between the side wall of the dish body 12 and the metal plate 6. One end of the adjusting part 5 is movably connected to one end of the sample support tube 2 close to the pipette connection part 21. The other end of the adjusting part 5 is rotatably connected to the side wall of the dish body 12. A second transmission part 33 is arranged inside the adjusting part 5. One ends of the first transmission part 23 and the second transmission part 33 are both movably connected to the inner side of the adjusting part 5, and the other ends of the first transmission part 23 and the second transmission part 33 are both movably connected to the metal plate 6. The internal components of the first transmission part 23 and the second transmission part 33 are the same.

[0037] Further, an annular guide rail 52 is opened on one side of the adjusting part 5 close to the metal plate 6. A plurality of fixing pins 62 slidably connected to the annular guide rail are installed on the metal plate 6, and the central position of the metal plate 6 is connected to the side wall of the housing assembly 1 through a fixing column 63.

[0038] In the above solution, the pipette sucks the sample together with agarose into the sample support tube 2 through the pipette connection part 21. Place this sample support device on the stage of an optical microscope so that the sample support tube 2 is located between the two lenses of the optical microscope. Add water to this support device until the lenses can be immersed, observe the body posture of the sample, and rotate the sample support tube 2 until the coronal plane of the sample is horizontal, then laser confocal scanning can be performed. This belongs to the conventional technical means in the prior art and will not be elaborated here.

[0039] Please refer to Figures 4 to 15, as an embodiment of the present invention, the first transmission part 23 includes a transmission gear 231, a magnet ring 232 and a movable sleeve 233. The transmission gear 231 is fixedly sleeved on the outer wall of the movable sleeve 233. Both ends of the movable sleeve 233 are designed with openings. A second limiting groove 235 extending along the axial direction is formed on the inner wall of the movable sleeve 233. One end of the outer wall of the sample support tube 2 close to the pipette connection part 21 is connected with a first limiting rib 24. The sample support tube 2 is inserted and connected in the second limiting groove 235 on the movable sleeve 233 through the first limiting rib 24. A magnet ring 232 is connected to one side of the transmission gear 231 close to the metal plate 6. The magnet ring 232 is adsorbed and connected with the metal plate 6. A return spring 236 is also connected between the transmission gear 231 and the metal plate 6. The return spring 236 is movably sleeved on the movable sleeve 233. The movable sleeve 233 is also movably sleeved in a second sleeving hole 61 formed on the surface of the metal plate 6. The transmission gear 231 is movably connected with the adjusting part 5. A lifting block 234 is installed at one end of the movable sleeve 233 passing through the second sleeving hole 61. In the initial state, the magnet ring 232 on the transmission gear 231 adsorbs on the surface of the metal plate 6 against the elastic force of the return spring 236. At this time, the transmission gear 231 is not connected with the meshing teeth 51 either. Only when it is necessary to drive the sample support tube 2, the telescopic part 3 or the pressing part 4 to move through the adjusting part, the magnet ring 232 and the metal plate 6 will be separated by pressing the lifting block 234. After the magnet ring 232 and the metal plate 6 are separated, there will still be an adsorption force between the two. However, due to the certain distance between the two, the adsorption force between the two is less than the elastic force of the return spring 236. That is to say, the return spring 236 can fix the position of the return spring 236 at the position meshing and connecting with the meshing teeth 51 on the adjusting part 5. When it is necessary to separate the transmission gear 231 and the adjusting part 5 again, only need to pull the lifting block 234 in the reverse direction with fingers, which has the characteristics of convenient operation and being convenient to adjust the movement state of the sample support tube 2, the telescopic part 3 or the pressing part 4 according to needs.

[0040] Further, the adjusting part 5 is designed as a rotating ring. The two end faces of the rotating ring are respectively rotationally connected with the side wall of the dish body 12 and the side wall of the dish body 12. A plurality of meshing teeth 51 meshing with the transmission gear 231 are uniformly arranged on the inner side of the rotating ring. The thickness value of the meshing teeth 51 is the same as the thickness value of the transmission gear 231. The thickness value of the transmission gear 231 is less than the gap value between the magnet ring 232 and the metal plate 6. During use, the staff can drive the transmission gear 231 to rotate through the meshing teeth 51 by rotating the outer ring of the rotating ring with fingers.

[0041] Further, sealing sleeves 17 are installed in both of the two through holes 16 on the dish body 12. A first limiting groove 171 is formed in the inner wall of one of the two sealing sleeves 17 close to the pipette connecting portion 21. The first limiting rib 24 on the sample support tube 2 is inserted and connected in the first limiting groove 171. While ensuring sealing, the sample support tube 2 can horizontally move in the first limiting groove 171 in the sealing sleeve 17 through the first limiting rib 24, and the rotating sample support tube 2 drives the first limiting groove 171 to rotate in one of the through holes 16 through the first limiting rib 24 and the first limiting groove 171.

[0042] In the above solution, the staff rotates the outer ring of the rotating ring with fingers, and can drive the transmission gear 231 to rotate through the meshing teeth 51. The rotating transmission gear 231 drives the sample support tube 2 to rotate through the movable sleeve 233, thus realizing the function of adjusting the observation angle or body posture of the sample.

[0043] Please refer to Figures 6 to 7 , as an embodiment of the present invention, the telescopic portion 3 further includes a threaded tube 31 and a threaded rod 32. One end of the threaded tube 31 is rotatably connected to the side wall of the dish body 12, and the end of the threaded tube 31 does not penetrate the side wall of the dish body 12. A second limiting rib 311 extending along the axial direction is connected to the outer wall of the threaded tube 31. The movable sleeve 233 in the second transmission portion 33 is connected to the threaded tube 31 through the second limiting rib 311. The threaded rod 32 is threadedly connected to and at the end of the threaded tube 31 away from the dish body 12. The other end of the threaded rod 32 is fixedly connected with a fixing plate 34. A first sleeving hole 35 is formed at the end of the fixing plate 34 away from the threaded rod 32. One end of the sample support tube 2 close to the pipette connecting portion 21 is connected with a telescopic portion connecting member 22. The telescopic portion connecting members 22 are distributed between the first limiting ribs 24 and the first limiting ribs 24. The fixing plate 34 is movably sleeved on the telescopic portion connecting member 22 through the first sleeving hole 35.

[0044] In the above solution, the telescopic part connecting piece can be designed as a sleeve with an annular groove on its surface. The first sleeve hole 35 is movably sleeved in the annular groove. The sleeve is fixedly sleeved on the sample support tube 2. Due to the design of the annular groove, when the sample support tube 2 rotates, the sleeve can rotate in the first sleeve hole 35 of the fixing plate 34. And when the fixing plate 34 moves horizontally, it can drive the sample support tube 2 to move together. Of course, the telescopic part connecting piece can also be designed in other structural forms, which is not limited here and will not be elaborated further. When in use, when the staff drives the sample support tube 2 to rotate around its own axis by pressing the first transmission part 23 to make it meshed and connected with the adjusting part 5, the staff can also drive the sample support tube 2 to move linearly along its own axis by pressing the second transmission part 33 to make it meshed and connected with the adjusting part 5. It can not only adjust the posture of the sample, but also adjust the horizontal position of the sample support tube 2 in the dish body 12 or under the lens of the optical microscope. Compared with the way of directly moving the dish body 12, this technical solution has the characteristics of high stability and accuracy through the way of gear meshing and screw-driven feeding, and can more accurately adjust the distance between the sample support tubes 2 between the lenses of the optical microscope, thereby improving the observation quality and observation effect.

[0045] Please refer to Figure 2 、 Figures 8 to 13 As an embodiment of the present invention, the housing assembly 1 further includes a water storage bin 13. The water storage bin 13 is located directly below the dish body 12. A partition plate 11 is provided between the dish body 12 and the water storage bin 13. One end of the partition plate 11 is provided with a water permeable hole 14 communicating with the dish body 12. A sealing cover 15 is installed in the water permeable hole 14.

[0046] Furthermore, it further includes a pressing part 4, a third transmission part 42 and a piston 44. The lead screw 41 is installed on the side walls at both ends of the water storage bin 13. A third limit rib 411 extending along the axis is connected to the side wall of the lead screw 41 near the adjusting part 5 and exposed outside the side wall of the water storage bin 13. The third transmission part 42 is consistent with the internal components of the first transmission part 23 or the second transmission part 33. The movable sleeve 233 in the third transmission part 42 is connected to the lead screw 41 through the third limit rib 411, and the transmission gear 231 in the third transmission part 42 is meshed and connected with the adjusting part 5. The third transmission part 42 is also installed on the metal plate 6 through the movable sleeve 233. The piston 44 is threadedly connected to the lead screw 41, and the side wall of the piston 44 is in close contact with the inner wall of the water storage bin 13. A fixing rod 43 parallel to the lead screw 41 is also fixedly connected to the inner wall of the water storage bin 13. The piston 44 is also movably sleeved on the fixing rod 43.

[0047] Further, the first transmission part 23, the second transmission part 33 and the third transmission part 42 are all distributed between the adjusting part 5 and the metal plate 6, and the first transmission part 23, the second transmission part 33 and the third transmission part 42 are symmetrically distributed about the center of the adjusting part 5. The sizes of the transmission gears 231 in the first transmission part 23, the second transmission part 33 and the third transmission part 42 and the diameters of the movable sleeves 233 do not need to be the same and can be designed according to actual needs, so no further description will be given here.

[0048] In the above solution, the sample support tube 2, the piston 44, the dish body 12, the water storage chamber 13 and the partition 11 are all made of materials with good light transmittance, which can maximize the light transmission of the optical microscope through the dish body 12, the water storage chamber 13 and the partition 11. When the light of the optical microscope irradiates from bottom to top, in order to prevent the lead screw 41 and the fixing rod 43 from blocking the sample support tube 2, the lead screw 41 and the fixing rod 43 are arranged on both sides below the lead screw 41 and the fixing rod 43. When water needs to be injected into the dish body 12, the sealing cover 15 is opened. The staff only needs to press the lifting block 234 downward with a finger during use to connect the transmission gear 231 in the third transmission part 42 with the meshing teeth 51 on the adjusting part 5, and then drive the lead screw 41 to rotate by rotating the adjusting part 5. The rotating lead screw 41 can drive the piston 44 to move towards the water permeable hole 14. The moving piston 44 can squeeze the water in the water storage chamber 13 into the upper dish body 12. When the water in the dish body 12 reaches a certain height, use a finger to pull the lifting block 234 again to disengage the transmission gear 231 in the third transmission part 42 from the meshing teeth 51 on the adjusting part 5. When it is necessary to adjust the rotation angle and horizontal position of the sample support tube 2 by rotating the adjusting part 5, the lead screw 41 will not be driven to rotate, which has the characteristic of being convenient for injecting water into the dish body 12. When the sample observation is completed, repeat the above operation again, and the water in the dish body 12 can be drained back into the water storage chamber 13 by moving the piston 44 in the reverse direction, thus realizing the function of recycling water and having the characteristics of energy conservation and resource recycling. Of course, a water injection port for injecting water into the interior will be installed on the outside of the water storage chamber 13, and the structure here can be designed according to needs, so no further description will be given.

[0049] In addition to the functions described above, the pressing part 4 has another function, that is, by moving the piston 44, it can also achieve the purpose of adjusting the water level in the dish body 12 according to the height of the lens in the dish body 12, which has the characteristic of strong practicability.

[0050] Working principle: The pipette sucks the sample together with agarose into the sample support tube 2 through the pipette connection part 21. The sample support device is placed on the stage of the optical microscope, and the sample support tube 2 is located between the two lenses of the optical microscope. When the operator drives the sample support tube 2 to rotate around its own axis by pressing the first transmission part 23 to engage it with the adjusting part 5, the operator can also drive the sample support tube 2 to move linearly along its own axis by pressing the second transmission part 33 to engage it with the adjusting part 5. This can not only adjust the posture of the sample, but also adjust the horizontal position of the sample support tube 2 in the dish body 12 or under the lens of the optical microscope. Compared with the method of directly moving the dish body 12, this technical solution has the characteristics of high stability and accuracy through gear meshing and screw-driven feeding, and can more accurately adjust the distance between the sample support tube 2 between the lenses of the optical microscope. When it is necessary to inject water into the dish body 12, open the sealing cover 15. The operator only needs to press the lifting block 234 downward with a finger during use to connect the transmission gear 231 in the third transmission part 42 with the meshing teeth 51 on the adjusting part 5, and then drive the screw rod 41 to rotate by rotating the adjusting part 5. The rotating screw rod 41 can drive the piston 44 to move towards the water permeable hole 14. The moving piston 44 can squeeze the water in the water storage chamber 13 into the upper dish body 12. When the water in the dish body 12 reaches a certain height, use a finger to pull the lifting block 234 again to disengage the transmission gear 231 in the third transmission part 42 from the meshing teeth 51 on the adjusting part 5. When it is necessary to adjust the rotation angle and horizontal position of the sample support tube 2 by rotating the adjusting part 5, the screw rod 41 will not be driven to rotate, which has the characteristic of being convenient for injecting water into the dish body 12. When the sample observation is completed, repeat the above operations again, and the water in the dish body 12 can be drained back into the water storage chamber 13 by moving the piston 44 in the reverse direction. In addition, by moving the piston 44, the water level in the dish body 12 can be adjusted according to the height of the lens in the dish body 12.

[0051] In summary, when the staff drives the sample support tube 2 to rotate around its own axis by pressing the first transmission part 23 to engage it with the adjusting part 5, it is also possible to drive the sample support tube 2 to move linearly along its own axis by pressing the second transmission part 33 to engage it with the adjusting part 5 again. This not only can adjust the body posture of the sample, but also can adjust the horizontal position of the sample support tube 2 in the dish body 12 or under the lens of the optical microscope. Compared with the method of directly moving the dish body 12, this technical solution has the characteristics of high stability and accuracy through the way of gear meshing and screw-driven feeding, and can more accurately adjust the distance between the sample support tubes 2 between the lenses of the optical microscope, thereby improving the observation quality and observation effect, and solving the problem of poor adjustability of the existing sample support device for microscopic observation.

[0052] It should be specifically noted that although this specification is described according to the implementation manners, not every implementation manner only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other implementation manners that can be understood by those skilled in the art. The above-described embodiments only express the preferred implementation manners of this technical solution, and the description is relatively specific and detailed, but it should not be construed as a limitation to the patent scope of this technical solution. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications, improvements and substitutions can still be made, and these all belong to the protection scope of this technical solution.

Claims

1. A sample support device for microscopic observation, comprising a housing assembly (1) and a sample support tube (2), characterized in that, A dish body (12) is arranged inside the housing assembly (1). The sample support tube (2) is movably connected in through holes (16) formed in the side walls at both ends of the dish body (12). One end of the sample support tube (2) is provided with a pipette connection part (21). A first transmission part (23) is movably sleeved on one side of the sample support tube (2) close to the pipette connection part (21). The first transmission part (23) is distributed between the side wall of the dish body (12) and the pipette connection part (21). It further includes a telescopic part (3), an adjusting part (5) and a metal plate (6). The metal plate (6) is fixedly connected to the side wall of the dish body (12). The adjusting part (5) is rotatably connected between the side wall of the dish body (12) and the metal plate (6). One end of the adjusting part (5) is movably connected to one end of the sample support tube (2) close to the pipette connection part (21). The other end of the adjusting part (5) is rotatably connected to the side wall of the dish body (12). A second transmission part (33) is arranged inside the adjusting part (5). One ends of the first transmission part (23) and the second transmission part (33) are both movably connected to the inner side of the adjusting part (5), and the other ends of the first transmission part (23) and the second transmission part (33) are both movably connected to the metal plate (6). The internal components of the first transmission part (23) and the second transmission part (33) are the same; The first transmission part (23) includes a transmission gear (231), a magnet ring (232) and a movable sleeve (233). The transmission gear (231) is fixedly sleeved on the outer wall of the movable sleeve (233). Both ends of the movable sleeve (233) are designed with openings. A second limiting groove (235) extending along the axial direction is formed in the inner wall of the movable sleeve (233). A first limiting rib (24) is connected to one end of the outer wall of the sample support tube (2) close to the pipette connection part (21). The sample support tube (2) is inserted and connected in the second limiting groove (235) on the movable sleeve (233) through the first limiting rib (24). A magnet ring (232) is connected to one side of the transmission gear (231) close to the metal plate (6). The magnet ring (232) is adsorbed and connected to the metal plate (6). A return spring (236) is further connected between the transmission gear (231) and the metal plate (6). The return spring (236) is movably sleeved on the movable sleeve (233). The movable sleeve (233) is also movably sleeved in a second sleeving hole (61) formed on the surface of the metal plate (6). The transmission gear (231) is movably connected to the adjusting part (5). A lifting block (234) is installed at one end of the movable sleeve (233) penetrating through the second sleeving hole (61); The adjusting member (5) is designed as a rotating ring. Two end faces of the rotating ring are respectively rotatably connected to the side wall of the dish body (12) and the side wall of the dish body (12). A plurality of engaging teeth (51) engaged with the transmission gear (231) are uniformly arranged on the inner side of the rotating ring. The thickness value of the engaging teeth (51) is the same as the thickness value of the transmission gear (231). The thickness value of the transmission gear (231) is less than the clearance value between the magnet ring (232) and the metal plate (6).

2. The sample support device for microscopic observation according to claim 1, characterized in that, The telescopic part (3) further includes a threaded pipe (31) and a threaded rod (32). One end of the threaded pipe (31) is rotatably connected to the side wall of the dish body (12), and the end of the threaded pipe (31) does not penetrate the side wall of the dish body (12). A second limiting rib (311) extending along the axial direction is connected to the outer wall of the threaded pipe (31). The movable sleeve (233) in the second transmission part (33) is connected to the threaded pipe (31) through the second limiting rib (311). The threaded rod (32) is threadedly connected to the end of the threaded pipe (31) far from the dish body (12). The other end of the threaded rod (32) is fixedly connected with a fixing plate (34). A first sleeving hole (35) is opened at the end of the fixing plate (34) far from the threaded rod (32). One end of the sample support tube (2) close to the pipette connection part (21) is connected with a telescopic part connecting piece (22). The telescopic part connecting pieces (22) are distributed between the first limiting ribs (24) and the first limiting ribs (24). The fixing plate (34) is movably sleeved on the telescopic part connecting piece (22) through the first sleeving hole (35).

3. The sample support device for microscopic observation according to claim 2, wherein, Sealing sleeves (17) are installed in both through holes (16) on the dish body (12). A first limiting groove (171) is opened on the inner wall of one of the two sealing sleeves (17) close to the pipette connection part (21). The first limiting rib (24) on the sample support tube (2) is inserted and connected in the first limiting groove (171).

4. The sample support device for microscopic observation according to claim 2, wherein, The housing assembly (1) further includes a water storage chamber (13). The water storage chamber (13) is located directly below the dish body (12). A partition plate (11) is arranged between the dish body (12) and the water storage chamber (13). A water permeable hole (14) communicating with the dish body (12) is opened at one end of the partition plate (11). A sealing cover (15) is installed in the water permeable hole (14) The described sample support device for microscopic observation.

5. The sample support device for microscopic observation according to claim 4, characterized in that, It further includes a pressing part (4), a lead screw (41), a third transmission part (42) and a piston (44). The lead screw (41) is installed on the side walls at both ends of the water storage bin (13). A third limiting rib (411) extending along the axial direction is connected to the side wall of the lead screw (41) near the adjusting part (5) and exposed at one end of the side wall of the water storage bin (13). The third transmission part (42) is consistent with the internal components of the first transmission part (23) or the second transmission part (33). The movable sleeve (233) in the third transmission part (42) is connected to the lead screw (41) through the third limiting rib (411), and the transmission gear (231) in the third transmission part (42) is meshed and connected to the adjusting part (5). The third transmission part (42) is also installed on the metal plate (6) through the movable sleeve (233). The piston (44) is threadedly connected to the lead screw (41), and the side wall of the piston (44) is in close contact with the inner wall of the water storage bin (13). A fixed rod (43) parallel to the lead screw (41) is fixedly connected to the inner wall of the water storage bin (13). The piston (44) is also movably sleeved on the fixed rod (43).

6. The sample support device for microscopic observation according to claim 5, wherein, The first transmission part (23), the second transmission part (33) and the third transmission part (42) are all distributed between the adjusting part (5) and the metal plate (6), and the first transmission part (23), the second transmission part (33) and the third transmission part (42) are symmetrically distributed about the center of the adjusting part (5).

7. The sample support device for microscopic observation according to claim 6, characterized in that, An annular guide rail (52) is provided on one side of the adjusting part (5) close to the metal plate (6). A plurality of fixed pins (62) slidably connected to the annular guide rail are installed on the metal plate (6), and the central position of the metal plate (6) is connected to the side wall of the housing assembly (1) through a fixed column (63).

Citation Information

Patent Citations

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