Tissue dissociation device

Abstract

The application discloses a tissue dissociation device, which comprises a main body mechanism and a power mechanism. The main body mechanism comprises a dissociation assembly, a liquid containing assembly and a conveying assembly. The dissociation assembly is used for grinding a tissue block to obtain a slurry solution. The conveying assembly is used for conveying the slurry solution from the dissociation assembly to the liquid containing assembly. The power mechanism comprises a driving assembly and a connecting assembly. The driving assembly comprises a dissociation driving part and a conveying driving part. The connecting assembly comprises a dissociation connecting structure and a conveying connecting structure. The dissociation connecting structure is used for drivingly connecting the dissociation driving part and the dissociation assembly. The conveying connecting structure is used for drivingly connecting the conveying driving part and the conveying assembly. The driving assembly and the main body mechanism are drivingly connected through the connecting assembly, so that the driving assembly can provide power for the main body mechanism. Meanwhile, the connecting assembly can also serve as a quick connection structure, thereby realizing quick disassembly and assembly of the driving assembly and the main body mechanism and making the replacement of the main body mechanism more convenient.

Description

Technical Field

[0001] This invention relates to the field of cell dissociation technology, and in particular to a tissue dissociation device. Background Technology

[0002] Single-cell sequencing is a new technology that performs high-throughput sequencing analysis of the genome, transcriptome, and epigenome at the single-cell level. It can reveal the gene structure and gene expression status of individual cells, reflecting intercellular heterogeneity and playing an important role in fields such as oncology, developmental biology, microbiology, and neuroscience. However, existing tissue dissociation devices for obtaining single-cell suspensions are complex in structure and non-disassemblyable. When components are damaged, individual parts cannot be replaced, resulting in resource waste. Summary of the Invention

[0003] The main objective of this invention is to propose a tissue dissociation device that addresses the problem of existing tissue dissociation devices being complex in structure and having individual components that cannot be disassembled, thus leading to the inability to replace individual components and consequently wasting resources.

[0004] To achieve the above objectives, the present invention provides a tissue dissociation device, comprising:

[0005] The main structure includes a dissociation component, a liquid-holding component, and a conveying component. The dissociation component is used to grind tissue blocks to obtain a plasma solution, and the conveying component is used to transport the plasma solution from the dissociation component to the liquid-holding component; and...

[0006] A power mechanism includes a drive assembly and a connection assembly. The drive assembly includes a disengaging drive element and a transmission drive element. The connection assembly includes a disengaging connection structure and a transmission connection structure. The disengaging connection structure is used to drive the disengaging drive element and the disengaging assembly, and the transmission connection structure is used to drive the transmission drive element and the transmission assembly.

[0007] Optionally, the dissociation component includes:

[0008] The dissociation chamber has a dissociation cavity for holding the tissue block; and,

[0009] A dissociation blade is rotatably mounted in the dissociation chamber along an axis extending vertically, and is used to grind the tissue block to obtain a plasma solution;

[0010] The dissociation connection structure is a transmission connection between the dissociation drive and the dissociation blade.

[0011] Optionally, the dissociation drive includes a dissociation motor having a dissociation output shaft arranged in a vertical direction;

[0012] Correspondingly, the dissociation connection structure includes a locking pin and a locking slot that fit together, wherein one of the locking pin and the locking slot is located on the dissociation output shaft and the other is located on the dissociation blade.

[0013] Optionally, the tissue dissociation device further includes a guide structure, which includes a first annular protrusion and a second annular protrusion that can be movably sleeved in the vertical direction. The first annular protrusion and the second annular protrusion are disposed opposite to each other and are respectively located on the dissociation output shaft and the dissociation blade.

[0014] Optionally, the liquid-containing assembly includes a liquid-containing reservoir, the liquid-containing reservoir having a liquid-containing chamber;

[0015] The transmission component includes:

[0016] A power chamber, having a power cavity, wherein a power pipe is provided at the upper end of the power chamber connecting the power cavity and the liquid-containing cavity, and a power piston movable vertically is provided inside the power cavity; and,

[0017] A liquid delivery pipe, one end of which is connected to the dissociation assembly and the other end of which extends into the liquid holding chamber, is used to introduce the slurry solution from the dissociation assembly into the liquid holding chamber when the power piston moves downward.

[0018] The transmission connection structure provides a transmission connection between the transmission drive component and the power piston.

[0019] Optionally, the conveying drive includes a conveying cylinder having a piston rod movable in the vertical direction;

[0020] Correspondingly, the transmission connection structure includes a connecting rod, wherein:

[0021] One end of the connecting rod is provided with a threaded hole for threaded connection with the free end of the piston rod; and / or,

[0022] A magnetic attraction structure is provided between the other end of the connecting rod and the power piston.

[0023] Optionally, the dissociation assembly includes a dissociation chamber having a dissociation cavity, wherein the tissue dissociation device further includes a dissociation fluid storage chamber and a pushing component, the dissociation fluid storage chamber having a dissociation fluid storage cavity for storing the dissociation fluid, the dissociation fluid storage cavity communicating with the dissociation cavity, and the pushing component for delivering the dissociation fluid from the dissociation fluid storage cavity to the dissociation cavity; and / or,

[0024] The liquid-containing assembly includes a liquid-containing tank, which has a liquid-containing cavity. The tissue dissociation device further includes a termination liquid storage tank and a pushing component. The termination liquid storage tank has a termination liquid storage cavity for storing the termination liquid. The termination liquid storage cavity is connected to the liquid-containing cavity. The pushing component is used to deliver the termination liquid from the termination liquid storage cavity to the liquid-containing cavity.

[0025] Optionally, the driving component further includes a push driver;

[0026] The connection component further includes a push connection structure, which is used to drive the push driver and the push component.

[0027] Optionally, the power mechanism further includes a heating component disposed on the dissociation component for heating the dissociation component.

[0028] Optionally, the power mechanism further includes a controller;

[0029] The dissociation assembly further includes a tissue block detection assembly disposed in the dissociation assembly for detecting the state of the tissue block;

[0030] The controller is electrically connected to the drive component and the tissue block detection component, and is used to control the operation of the drive component according to the detection result of the tissue block detection component.

[0031] In the technical solution of this invention, the main body includes a dissociation component, a liquid-holding component, and a conveying component. The tissue block is ground into a slurry solution in the dissociation component, and the slurry solution is conveyed from the dissociation component to the liquid-holding component through the conveying component. A power mechanism is provided to provide power to the main body. The drive component and the main body are connected by a connecting component, so that the drive component can provide power to the main body. At the same time, the connecting component can also serve as a quick-connect structure, which not only enables quick assembly and disassembly of the drive component and the main body, making the replacement of the main body more convenient, but also allows the tissue dissociation device to be restored to operation simply by replacing the corresponding parts when the main body or the power mechanism is damaged, thereby saving resources. Attached Figure Description

[0032] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0033] Figure 1 This is a schematic diagram of a structure of an embodiment of the tissue dissociation device provided by the present invention;

[0034] Figure 2 for Figure 1 A top view of the tissue dissociation device in the diagram;

[0035] Figure 3 for Figure 2 Schematic cross-section view along the middle AA;

[0036] Figure 4 for Figure 3 A magnified schematic diagram of part B in the middle;

[0037] Figure 5 for Figure 2 Cross-sectional view of the center line CC;

[0038] Figure 6 for Figure 1 A structural diagram of the main body of the structure from another perspective.

[0039] Explanation of reference numerals in the accompanying drawings of the embodiments provided in this invention:

[0040]

[0041]

[0042] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0043] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0044] It should be noted that if the embodiments of the present invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0045] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0046] Single-cell sequencing is a new technology that performs high-throughput sequencing analysis of the genome, transcriptome, and epigenome at the single-cell level. It can reveal the gene structure and gene expression status of individual cells, reflecting intercellular heterogeneity and playing an important role in fields such as oncology, developmental biology, microbiology, and neuroscience. However, existing tissue dissociation devices for obtaining single-cell suspensions are complex in structure and non-disassemblyable. When components are damaged, individual parts cannot be replaced, resulting in resource waste.

[0047] In view of this, the present invention provides a tissue dissociation device. Figures 1 to 6 This is a specific embodiment of the tissue dissociation device provided by the present invention.

[0048] Please see Figures 1 to 6 The tissue dissociation device 100 includes a main body 1 and a power mechanism 2. The main body 1 includes a dissociation component 11, a liquid holding component 12, and a conveying component 13. The dissociation component 11 is used to grind tissue blocks to obtain a plasma solution, and the conveying component 13 is used to transport the plasma solution from the dissociation component 11 to the liquid holding component 12. The power mechanism 2 includes a drive component 21 and a connecting component 22. The drive component 21 includes a dissociation drive component 211 and a conveying drive component 212. The connecting component 22 includes a dissociation connection structure 221 and a conveying connection structure 222. The dissociation connection structure 221 is used to drive the dissociation drive component 211 and the dissociation component 11, and the conveying connection structure 222 is used to drive the conveying drive component 212 and the conveying component 13.

[0049] In the technical solution of the present invention, the main body mechanism 1 includes a dissociation component 11, a liquid holding component 12, and a conveying component 13. The tissue block is ground into a slurry solution in the dissociation component 11, and the slurry solution is conveyed from the dissociation component 11 to the liquid holding component 12 through the conveying component 13. A power mechanism 2 is provided to provide power to the main body mechanism 1. A connecting component 22 is provided to drive the drive component 21 and the main body mechanism 1, so that the drive component 21 can provide power to the main body mechanism 1. At the same time, the connecting component 22 can also serve as a quick-connect structure, which not only enables quick assembly and disassembly of the drive component 21 and the main body mechanism 1, making the replacement of the main body mechanism 1 more convenient, but also allows the tissue dissociation device 100 to be restored to work simply by replacing the corresponding parts when the main body mechanism 1 or the power mechanism 2 is damaged, thereby saving resources.

[0050] It should be noted that in this invention, the main body 1 is set as a disposable replaceable part. In this way, each time a single-cell suspension is prepared, only a new main body 1 needs to be replaced, which is not only convenient to operate, but also saves resources.

[0051] Specifically, please refer to Figure 3 and Figure 4 The dissociation assembly 11 includes a dissociation chamber 111 and a dissociation blade 112. The dissociation chamber 111 has a dissociation cavity for holding the tissue block. The dissociation blade 112 is rotatably mounted in the dissociation cavity along an axis extending vertically, for grinding the tissue block to obtain a paste solution. The dissociation connection structure 221 drives the dissociation drive 211 and the dissociation blade 112. In this embodiment, the tissue block is placed in the dissociation chamber 111, and the dissociation drive 211 drives the dissociation blade 112 to rotate to grind the tissue block, thereby obtaining the paste solution. At the same time, since the dissociation connection structure 221 drives the dissociation drive 211 and the dissociation blade 112, the dissociation drive 211 and the dissociation blade 112 can be quickly assembled and disassembled.

[0052] More specifically, a plurality of fixed blades are provided on the bottom wall of the dissociation chamber, and the plurality of fixed blades are arranged circumferentially. The dissociation blade 112 includes at least two first blades spaced circumferentially and spirally arranged in the up-down direction. A lysis gap is formed between the two first blades and the plurality of fixed blades. The two first blades and the plurality of fixed blades cooperate to grind the tissue block in the lysis gap to obtain a homogenized solution. It should be noted that when the tissue block is placed in the dissociation chamber, the dissociation drive 211 drives the dissociation blade 112 to rotate, so that the two first blades cooperate with the inner wall surface of the dissociation chamber and the plurality of fixed blades to grind the tissue block together, so that the tissue block is crushed to obtain a homogenized solution. At the same time, the user can achieve different effects with the plurality of fixed blades by adjusting the rotation speed and rotation direction of the lysis blade. When rotating forward, the tissue is broken up; when rotating backward, the tissue is incubated, thereby turning the tissue block into a homogenized solution. That is, by the interaction of the rotating dissociation blade 112 and the fixed blades, the lysis effect of the tissue block can be improved.

[0053] In this embodiment, the dissociation drive 211 includes a dissociation motor 2111, which has a dissociation output shaft arranged vertically. Correspondingly, the dissociation connection structure 221 includes a locking pin 2211 and a locking groove 2212 that fit together. One of the locking pin 2211 and the locking groove 2212 is located on the dissociation output shaft, and the other is located on the dissociation blade 112. The locking pin 2211 and the locking groove 2212 are correspondingly arranged between the dissociation output shaft and the dissociation blade 112, so that the dissociation output shaft and the dissociation blade 112 are connected. When the dissociation output shaft rotates, it can drive the dissociation blade 112 to rotate. At the same time, the structural design of the locking pin 2211 and the locking groove 2212 can realize the quick assembly and disassembly of the dissociation output shaft and the dissociation blade 112.

[0054] The present invention does not limit the specific installation positions of the locking post 2211 and the locking groove 2212. It can be that the free end of the dissociation output shaft has a mounting protrusion, and the locking groove 2212 is provided on the side of the mounting protrusion facing the dissociation blade 112. Correspondingly, the locking post 2211 is provided on the side of the dissociation blade 112 facing the dissociation output shaft. Specifically, in this embodiment, the locking groove 2212 is located on the side of the dissociation blade 112 facing the dissociation output shaft, and the locking post 2211 is located on the free end of the dissociation output shaft. This not only enables quick assembly and disassembly of the dissociation output shaft and the dissociation blade 112, but also better conforms to the structural design of the main body mechanism 1 and the power mechanism 2.

[0055] To facilitate smoother installation of the dissociation output shaft and the dissociation blade 112, the tissue dissociation device 100 further includes a guide structure 3 in this invention. The guide structure 3 includes a first annular protrusion 31 and a second annular protrusion 32 that can be movably fitted vertically. The first annular protrusion 31 and the second annular protrusion 32 are oppositely disposed and respectively located on the dissociation output shaft and the dissociation blade 112. It should be noted that in this embodiment, a mounting protrusion protrudes from the side of the dissociation blade 112 facing the dissociation output shaft, and the slot 2212 is located on the side of the mounting protrusion facing the dissociation output shaft. The locking post 2211 is located at the free end of the dissociation output shaft; the first annular protrusion 31 is arranged around the outside of the mounting protrusion and spaced apart from the mounting protrusion, and the second annular protrusion 32 is arranged around the outside of the locking post 2211 and spaced apart from the locking post 2211. The first annular protrusion 31 is movably sleeved on the outside of the second annular protrusion 32. That is, during the installation process, the first annular protrusion 31 and the second annular protrusion 32 cooperate with each other to guide the dissociation output shaft and the dissociation blade 112, so that the installation of the dissociation output shaft and the dissociation blade 112 is smoother and faster.

[0056] Please see Figure 2 and Figure 5The liquid-containing assembly 12 includes a liquid-containing tank 121, which has a liquid-containing cavity; the conveying assembly 13 includes a power chamber 131 and a delivery pipe 134; the power chamber 131 has a power cavity, and a power pipe 132 connecting the power cavity and the liquid-containing cavity is provided at the upper end of the power chamber 131; a power piston 133 movable vertically is provided in the power cavity; one end of the delivery pipe 134 is connected to the dissociation assembly 11, and the other end extends into the liquid-containing cavity, so as to guide the slurry solution from the dissociation assembly 11 into the liquid-containing cavity when the power piston 133 moves downward; wherein, the conveying connection structure 222 drives the conveying drive 212 and the power piston 133; that is, the liquid-containing cavity is connected to the dissociation assembly 11 through the delivery pipe 134. The components are connected to each other. The liquid-containing chamber is connected to the power chamber via the power pipe 132. A power piston 133, movable vertically, is installed in the power chamber. When the power piston 133 moves downwards, it draws air from the liquid-containing chamber into the power chamber via the power pipe 132. This reduces the air pressure in the liquid-containing chamber, allowing the slurry solution from the dissociation component 11 to be drawn into the liquid-containing chamber via the liquid delivery pipe 134, thus conveying the slurry solution from the dissociation component 11 to the liquid-containing chamber. Simultaneously, the conveying connection structure 222 drives the conveying drive component 212 and the power piston 133. This not only enables the movement of the power piston 133 but also allows for quick assembly and disassembly of the power piston 133 and the conveying drive component 212.

[0057] Specifically, the conveying drive component 212 includes a conveying cylinder 2121, which has a piston rod that can move vertically; correspondingly, the conveying connection structure 222 includes a connecting rod 2221; the connecting rod 2221 drives the piston rod and the power piston 133 to move vertically when the piston rod extends and retracts vertically, thereby realizing the conveying of the slurry solution.

[0058] In this invention, one end of the connecting rod 2221 is provided with a threaded hole for threaded connection with the free end of the piston rod; the free end of the piston rod is threadedly connected to one end of the connecting rod 2221, thereby making the connecting rod 2221 and the piston rod detachable.

[0059] In this invention, a magnetic attraction structure 2222 is provided between the other end of the connecting rod 2221 and the power piston 133. During installation, the connecting rod 2221 and the power piston 133 are attracted together by the magnetic attraction structure 2222. The piston rod can drive the power piston 133 to move through the connecting rod 2221. During disassembly, the connecting rod 2221 and the power piston 133 can be separated directly by external force. Thus, the structure is simple and the disassembly and assembly are convenient and quick.

[0060] It should be noted that the above two technical features can be set individually or simultaneously. Specifically, in this embodiment, both technical features are set simultaneously. That is, one end of the connecting rod 2221 is provided with a threaded hole for threaded connection with the free end of the piston rod; the other end of the connecting rod 2221 is provided with a magnetic attraction structure 2222 between it and the power piston 133. Thus, one end of the connecting rod 2221 is detachably connected to the piston rod by threading it with the free end of the piston rod. At the same time, the other end of the connecting rod 2221 and the power piston 133 are attracted together by the magnetic attraction structure 2222. The piston rod can drive the power piston 133 to move through the connecting rod 2221. During disassembly, the connecting rod 2221 and the power piston 133 can be separated directly by external force. Thus, the structure is simple and the disassembly and assembly are convenient and quick.

[0061] Please refer to further information. Figures 2 to 6 In this invention, the dissociation component 11 includes a dissociation chamber 111, which has a dissociation cavity for holding the tissue block; the tissue dissociation device 100 also includes a dissociation fluid storage chamber 14 and a pushing component 16. The dissociation fluid storage chamber 14 has a dissociation fluid storage cavity for storing the dissociation fluid, which is connected to the dissociation cavity. The pushing component 16 is used to deliver the dissociation fluid from the dissociation fluid storage cavity to the dissociation cavity. It should be noted that during the dissociation of the tissue block, the tissue block is placed in the dissociation cavity, the pushing component 16 delivers the dissociation fluid from the dissociation fluid storage cavity to the dissociation cavity, and the dissociation blade 112 rotates to grind the tissue block so that the tissue block is crushed. The dissociation fluid can dissolve the middle layer of the cell wall (pectin), thereby separating the cells. The tissue block containing the dissociation fluid is ground by the dissociation blade 112 to obtain a uniform plasma solution.

[0062] In this invention, the liquid-containing assembly 12 includes a liquid-containing tank 121, which has a liquid-containing cavity; the tissue dissociation device 100 further includes a termination fluid storage tank 15 and a pushing assembly 16. The termination fluid storage tank 15 has a termination fluid storage cavity for storing the termination fluid, which is connected to the liquid-containing cavity. The pushing assembly 16 is used to deliver the termination fluid from the termination fluid storage cavity to the liquid-containing cavity. It should be noted that after the tissue block is dissociated into the plasma solution, it is transported to the liquid-containing cavity. To avoid excessive dissociation caused by residual dissociation fluid in the plasma solution, which could lead to cell damage, the pushing assembly 16 delivers the termination fluid from the termination fluid storage cavity to the liquid-containing cavity, thereby stopping the dissociation of the plasma solution in the liquid-containing cavity and improving the quality of the dissociated cells.

[0063] It should be noted that the above two technical features can be set selectively or simultaneously. Specifically, in this embodiment, the above two technical features are set simultaneously, that is, the dissociation component 11 includes a dissociation chamber 111, which has a dissociation cavity for holding the tissue block; the liquid holding component 12 includes a liquid holding chamber 121, which has a liquid holding cavity; the tissue dissociation device 100 further includes a dissociation liquid storage chamber 14 and a pushing component 16, the dissociation liquid storage chamber 14 has a dissociation liquid storage cavity for storing the dissociation liquid, the dissociation liquid storage cavity is connected to the dissociation cavity, and the pushing component 16 is used to deliver the dissociation liquid from the dissociation liquid storage cavity to the dissociation cavity; the tissue dissociation device 100 further includes a termination liquid storage chamber 15 and a pushing component 16, the termination liquid storage chamber 15 has a termination liquid storage cavity for storing the termination liquid, the termination liquid storage cavity is connected to the liquid holding cavity. The pushing component 16 is used to deliver the terminating solution from the terminating solution storage chamber to the liquid holding chamber. In this embodiment, during the dissociation of the tissue block, the tissue block is placed in the dissociation chamber, and the pushing component 16 delivers the dissociation solution from the dissociation solution storage chamber to the dissociation chamber. The dissociation blade 112 rotates to grind the tissue block, so that the tissue block is crushed. The dissociation solution can dissolve the middle layer of the cell wall (pectin), thereby separating the cells. The tissue block with the dissociation solution is ground by the dissociation blade 112 to obtain a uniform plasma solution. At the same time, after the tissue block is dissociated into the plasma solution, it is transported to the liquid holding chamber. In order to avoid excessive dissociation caused by residual dissociation solution in the plasma solution, which would lead to cell damage, the pushing component 16 delivers the terminating solution from the terminating solution storage chamber to the liquid holding chamber, so that the plasma solution in the liquid holding chamber stops dissociation, thereby improving the quality of the dissociated cells.

[0064] Specifically, the driving component 21 further includes a push driving element 213; the connecting component 22 further includes a push connecting structure 223, which is used to drive the push driving element 213 and the push component 16; the push connecting structure 223 can be used as a quick-connect structure to realize the quick assembly and disassembly of the push driving element 213 and the push component 16.

[0065] Specifically, in this embodiment, the pushing component 16 includes a pushing pipe 161 and a pushing piston 162; the dissociation liquid storage chamber and the termination liquid storage chamber are respectively connected to the dissociation chamber and the liquid holding chamber through the pushing pipe 161, and the pushing pipe 161 is respectively disposed at the upper end of the dissociation liquid storage chamber 14 and the termination liquid storage chamber 15; the pushing piston 162 is disposed in both the dissociation liquid storage chamber and the termination liquid storage chamber, and the pushing piston 162 can move vertically within the dissociation liquid storage chamber and the termination liquid storage chamber, wherein the pushing connection structure 223 drives the pushing drive component 213 and the pushing piston 162; that is, the dissociation liquid storage chamber and the termination liquid storage chamber are respectively connected to the dissociation chamber and the liquid holding chamber through the pushing pipe 161, and when in the dissociation liquid storage chamber... When the push piston 162 moves upward, it can push the dissociated liquid in the dissociated liquid storage chamber upward. When the liquid level of the dissociated liquid is higher than the opening of the corresponding push pipe 161, the dissociated liquid can flow to the dissociated chamber through the corresponding push pipe 161, thereby realizing the pushing of the dissociated liquid. When the push piston 162 in the termination liquid storage chamber moves upward, it can push the termination liquid in the termination liquid storage chamber upward. When the liquid level of the termination liquid is higher than the opening of the corresponding push pipe 161, the termination liquid can flow to the liquid holding chamber through the corresponding push pipe 161. At the same time, since the push connection structure 223 drives the push drive 213 and the push piston 162, not only can the movement of the push piston 162 be realized, but also the quick assembly and disassembly of the push piston 162 and the push drive 213 can be realized.

[0066] Specifically, the pushing drive component 213 includes a pushing cylinder 2131, which has a pushing piston rod that can move vertically; correspondingly, the pushing connection structure 223 includes a pushing connecting rod; the pushing connecting rod drives the pushing piston rod and the pushing piston 162 to move vertically when the pushing piston rod extends and retracts vertically, thereby realizing the pushing of the dissociation liquid or the termination liquid.

[0067] More specifically, one end of the push connecting rod is provided with a threaded hole for threaded connection with the free end of the push piston rod; the other end of the push connecting rod is provided with a magnetic attraction structure 2222 between it and the push piston 162. Thus, one end of the push connecting rod is detachably connected to the push piston rod by threading it with the free end of the push piston rod. At the same time, the other end of the push connecting rod and the push piston 162 are attracted together by the magnetic attraction structure 2222. The push piston rod can drive the push piston 162 to move through the push connecting rod. During disassembly, the push connecting rod and the push piston 162 can be separated directly by external force. Thus, the structure is simple and the disassembly and assembly are convenient and quick.

[0068] It should be noted that the tissue block needs to be dissociated at a certain preset temperature. Therefore, in this invention, the power mechanism 2 also includes a heating component 4 disposed on the dissociation component 11 to heat the dissociation component 11. By setting the heating component 4 so that the dissociation component 11 is at the preset temperature, not only can the dissociation speed of the tissue block be improved, but also the dissociation effect of the tissue block can be improved.

[0069] Specifically, the dissociation assembly 11 includes a dissociation chamber 111, and the heating assembly 4 is disposed on the outside of the dissociation chamber 111 to heat the dissociation fluid and tissue block in the dissociation chamber 111 so that the dissociation fluid reaches the preset temperature, which can not only improve the dissociation speed of the tissue block, but also improve the dissociation effect of the tissue block.

[0070] Based on the above structure, the specific steps for preparing a single-cell suspension are as follows:

[0071] Tissue preparation: Add 1×PBS solution to a six-well plate to wash the sample stored in the tissue preservation solution. Take an appropriate amount of the washed sample and add it to the dissociation solution. Use surgical scissors to cut the tissue into tissue blocks of appropriate size.

[0072] Tissue lysis: Place the dissociation solution containing the tissue in the dissociation chamber and act at 37°C for 15-40 minutes. During this process, the dissociation blade needs to rotate forward every 5 minutes to break up the tissue. Then, the dissociation blade rotates in the opposite direction for the same amount of time to incubate the tissue. The optimal method is to completely digest the tissue block and form a homogenous solution.

[0073] Transfer of homogenized solution: The homogenized solution is subjected to negative pressure by the power chamber and the liquid delivery pipe, and enters the liquid holding chamber after being filtered through a 40-70μm sterile cell sieve;

[0074] An equal volume of termination solution is added to the filtrate in the liquid-containing chamber to complete the dissociation termination and obtain a cell suspension.

[0075] Add an equal volume of erythrocyte lysis buffer to the cell suspension, invert to mix, and incubate at room temperature for 2-7 minutes.

[0076] Add an equal volume of 1640 solution to stop erythrocyte lysis, invert and mix well, then incubate at 300g for 5 minutes at 4°C. Remove the supernatant to obtain a single-cell precipitate, and resuspend to obtain a single-cell suspension.

[0077] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made under the concept of the present invention using the contents of the present invention specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A tissue dissociation device, characterized in that, include: The main structure includes a dissociation component, a liquid holding component, and a conveying component. The dissociation component is used to grind tissue blocks to obtain a plasma solution, and the conveying component is used to transport the plasma solution from the dissociation component to the liquid holding component. as well as, A power mechanism includes a drive assembly and a connection assembly. The drive assembly includes a disengaging drive element and a transmission drive element. The connection assembly includes a disengaging connection structure and a transmission connection structure. The disengaging connection structure is used to drive the disengaging drive element and the disengaging assembly. The transmission connection structure is used to drive the transmission drive element and the transmission assembly. The liquid-containing assembly includes a liquid-containing reservoir, which has a liquid-containing chamber. The transmission component includes: A power chamber, having a power cavity, wherein a power pipe is provided at the upper end of the power chamber connecting the power cavity and the liquid-containing cavity, and a power piston movable vertically is provided inside the power cavity; and, A liquid delivery pipe, one end of which is connected to the dissociation assembly and the other end of which extends into the liquid holding chamber, is used to introduce the slurry solution from the dissociation assembly into the liquid holding chamber when the power piston moves downward. The transmission connection structure provides a transmission connection between the transmission drive and the power piston. The dissociation assembly includes a dissociation chamber, which has a dissociation cavity. The tissue dissociation device further includes a dissociation fluid storage chamber and a first pushing component. The dissociation fluid storage chamber has a dissociation fluid storage cavity for storing the dissociation fluid. The dissociation fluid storage cavity is connected to the dissociation cavity. The first pushing component is used to deliver the dissociation fluid from the dissociation fluid storage cavity to the dissociation cavity. The tissue dissociation device further includes a termination fluid storage chamber and a second pushing component. The termination fluid storage chamber has a termination fluid storage cavity for storing the termination fluid, and the termination fluid storage cavity is connected to the liquid holding cavity. The second pushing component is used to deliver the termination fluid from the termination fluid storage cavity to the liquid holding cavity.

2. The tissue dissociation device as described in claim 1, characterized in that, The dissociation component includes: A dissociation chamber for holding the tissue block; and, A dissociation blade is rotatably mounted in the dissociation chamber along an axis extending vertically, and is used to grind the tissue block to obtain a plasma solution; The dissociation connection structure is a transmission connection between the dissociation drive and the dissociation blade.

3. The tissue dissociation device as described in claim 2, characterized in that, The dissociation drive includes a dissociation motor, which has a dissociation output shaft arranged in a vertical direction; Correspondingly, the dissociation connection structure includes a locking pin and a locking slot that fit together, wherein one of the locking pin and the locking slot is located on the dissociation output shaft and the other is located on the dissociation blade.

4. The tissue dissociation device as described in claim 3, characterized in that, The tissue dissociation device further includes a guide structure, which includes a first annular protrusion and a second annular protrusion that can be movably sleeved in the vertical direction. The first annular protrusion and the second annular protrusion are arranged opposite to each other and are respectively located on the dissociation output shaft and the dissociation blade.

5. The tissue dissociation device as described in claim 4, characterized in that, The conveying drive includes a conveying cylinder, which has a piston rod that can move vertically. Correspondingly, the transmission connection structure includes a connecting rod, wherein: One end of the connecting rod is provided with a threaded hole for threaded connection with the free end of the piston rod; and / or, A magnetic attraction structure is provided between the other end of the connecting rod and the power piston.

6. The tissue dissociation device as described in claim 1, characterized in that, The power mechanism also includes a heating component disposed on the dissociation component for heating the dissociation component.

7. The tissue dissociation device as described in claim 1, characterized in that, The power mechanism also includes a controller; The dissociation assembly further includes a tissue block detection assembly disposed in the dissociation assembly for detecting the state of the tissue block; The controller is electrically connected to the drive component and the tissue block detection component, and is used to control the operation of the drive component according to the detection result of the tissue block detection component.

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