An extracorporeal simulated circulatory system of cerebrospinal fluid

Abstract

The present invention relates to the field of biomedical technology, and in particular to an in vitro simulated cerebrospinal fluid circulation system; the in vitro simulated cerebrospinal fluid circulation system comprises a cerebrospinal fluid supply device, a cerebrospinal fluid power regulating device, a cerebrospinal fluid impact simulation device and a connecting pipeline; the system of the present application simulates a brain functional area through an impact area of ​​a cerebrospinal fluid impact simulation device, and a model structure to be tested is placed in the impact area; cerebrospinal fluid is supplied through the cerebrospinal fluid supply device, and the cerebrospinal fluid power regulating device controls and regulates the liquid flow rate and flow velocity in the connecting pipeline, and the liquid in the tube impacts the model structure to be tested located in the impact area at different flow velocities, and the cell number and differentiation index of the model structure to be tested before and after the impact are compared, so as to achieve the simulation of the influence of the flow direction and velocity of cerebrospinal fluid in vivo on cells and the analysis of the cell influence.

Description

Technical Field

[0001] The invention relates to the field of biomedical technology, and in particular to an extracorporeal simulated cerebrospinal fluid circulation system. Background Art

[0002] Pathogenic examination of cerebrospinal fluid is the key to diagnosing central nervous system infections, but for a long time, the sensitivity and specificity of virological diagnostic technology have been very poor, far from meeting clinical requirements; medical experimental research on cerebrospinal fluid has become the most valuable research method and standard for virological diagnosis in the biomedical field; existing laboratory-related equipment does not have in vitro simulation tools to attempt to simulate the related activities of cerebrospinal fluid; therefore, the industry is in urgent need of an in vitro simulation circulation system for cerebrospinal fluid. Summary of the invention

[0003] The main purpose of the present invention is to provide an in vitro simulated cerebrospinal fluid circulation system, aiming to solve the problem of the influence of the flow velocity and direction of cerebrospinal fluid in vivo on cells by simulating it in vitro.

[0004] To achieve the above-mentioned purpose, the present invention provides an extracorporeal simulated cerebrospinal fluid circulation system, comprising a cerebrospinal fluid supply device, a cerebrospinal fluid power regulating device, a cerebrospinal fluid impact simulation device and a connecting pipeline; the connecting pipeline comprises a first pipeline, a second pipeline and a third pipeline;

[0005] The cerebrospinal fluid supply device is provided with a liquid inlet and a liquid outlet; one end of the cerebrospinal fluid impact simulation device is provided with an input port, and the other end of the cerebrospinal fluid impact simulation device is provided with an output port;

[0006] The liquid outlet is connected to the input end of the cerebrospinal fluid power regulating device through a first pipeline, the output end of the cerebrospinal fluid power regulating device is connected to the input port of the cerebrospinal fluid impact simulation device through a second pipeline, and the output port of the cerebrospinal fluid impact simulation device is connected to the liquid inlet of the cerebrospinal fluid supply device through a third pipeline;

[0007] The cerebrospinal fluid power regulating device is used to control and regulate the flow rate and flow rate of the liquid in the second pipeline;

[0008] The cerebrospinal fluid impact simulation device is provided with an impact area, in which a model structure to be tested is placed; the cerebrospinal fluid impact simulation device is used to enable the model structure to be tested to receive impact and to test the impact effect on the model structure to be tested.

[0009] Furthermore, the cerebrospinal fluid supply device is provided with a fluid infusion port, which is connected to a first valve, and the first valve controls the opening and closing of the fluid infusion port; the fluid infusion port is used to inject liquid into the cerebrospinal fluid supply device.

[0010] Furthermore, the cerebrospinal fluid supply device is provided with an exhaust port, to which a filter ventilation valve is connected; the filter ventilation valve controls the opening and closing of the exhaust port to filter pollutants in the atmosphere and gas exchange of the cerebrospinal fluid supply device.

[0011] Furthermore, the cerebrospinal fluid supply device is a closed box structure, with a liquid inlet at one end of the box, a liquid outlet at the other end of the box, and a liquid inlet and an exhaust port at the top of the box.

[0012] Furthermore, the cerebrospinal fluid supply device is a closed bottle structure, which includes a bottle body and a bottle stopper, the bottle stopper seals the bottle mouth of the bottle body; the bottle stopper is respectively provided with a penetrating liquid inlet hole and a liquid outlet hole, the liquid outlet hole is inserted with a liquid outlet tube, and the liquid inlet hole is inserted with a liquid inlet tube; the bottle stopper is respectively provided with a penetrating liquid infusion hole and an exhaust hole, the liquid infusion hole is inserted with a liquid infusion tube, and the first valve is arranged on the liquid infusion tube outside the bottle body; the exhaust hole is inserted with an exhaust pipe, and the filter ventilation valve is arranged on the exhaust pipe outside the bottle body.

[0013] Furthermore, the cerebrospinal fluid power regulating device is an adjustable flow power pump device.

[0014] Furthermore, the cerebrospinal fluid impact simulation device includes a circular tube, with closed joints at both ends of the circular tube respectively, and the conducting ports of the closed joints at both ends of the circular tube are respectively connected to the second pipeline and the third pipeline; an impact area is provided in the circular tube, and the impact area is used to simulate the damaged part of the brain.

[0015] Furthermore, the impact area is a concave structure arranged in the circular tube according to the concave and / or convex structure of the brain functional area.

[0016] Furthermore, a flow meter and a pressure detector are provided on the second pipeline; and a pressure reducing valve is provided on the third pipeline.

[0017] Furthermore, the model structure to be tested includes a model support and cells attached to the model support; the model support is simulated and printed using a 3D printing device.

[0018] Beneficial Effects

[0019] In the above technical scheme, the brain functional area is simulated by the impact area of ​​the cerebrospinal fluid impact simulation device, and the model structure to be tested is placed in the impact area; and the cerebrospinal fluid is supplied by the cerebrospinal fluid supply device, and the cerebrospinal fluid power regulating device controls and regulates the liquid flow and flow rate in the connecting pipeline. The liquid in the connecting pipeline impacts the model structure to be tested located in the impact area at different flow rates, and the cell number and differentiation index of the model structure to be tested before and after the impact are compared, so as to realize the simulation of the influence of the flow direction and speed of cerebrospinal fluid in the body on the cells and the analysis of the influence on the cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the structure of the in vitro simulated cerebrospinal fluid circulation system of the present invention;

[0022] Figure 2 for Figure 1 Schematic diagram of the structure of the cerebrospinal fluid supply device;

[0023] Figure 3 for Figure 1 Schematic diagram of the structure of the cerebrospinal fluid impact simulation device.

[0024] Among them, 1. cerebrospinal fluid supply device, 1.1. bottle body, 1.2. bottle stopper, 1.3. first valve, 1.4. filter ventilation valve, 1.5. liquid outlet pipe, 1.6. liquid inlet pipe, 2. cerebrospinal fluid power regulation device, 3. cerebrospinal fluid impact simulation device, 3.1. glass round tube, 3.2. closed joint, 3.3. impact area, 4. first pipeline, 5. second pipeline, 6. third pipeline.

[0025] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0028] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0029] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In addition, the technical solutions between the various embodiments of the present invention can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0031] like Figures 1 to 3 As shown, the cerebrospinal fluid extracorporeal simulation circulation system proposed by the present invention comprises a cerebrospinal fluid supply device 1, a cerebrospinal fluid power regulating device 2, a cerebrospinal fluid impact simulation device 3 and a connecting pipeline; the connecting pipeline comprises a first pipeline 4, a second pipeline 5 and a third pipeline 6;

[0032] The cerebrospinal fluid supply device is provided with a liquid inlet and a liquid outlet; one end of the cerebrospinal fluid impact simulation device is provided with an input port, and the other end of the cerebrospinal fluid impact simulation device is provided with an output port;

[0033] The liquid outlet is connected to the input end of the cerebrospinal fluid power regulating device through a first pipeline, the output end of the cerebrospinal fluid power regulating device is connected to the input port of the cerebrospinal fluid impact simulation device through a second pipeline, and the output port of the cerebrospinal fluid impact simulation device is connected to the liquid inlet of the cerebrospinal fluid supply device through a third pipeline;

[0034] The cerebrospinal fluid power regulating device is used to control and regulate the flow rate and flow rate of the liquid in the second pipeline;

[0035] The cerebrospinal fluid impact simulation device is provided with an impact area 3.3, in which the model structure to be tested is placed; the cerebrospinal fluid impact simulation device is used to enable the model structure to be tested to receive impact and to test the impact effect on the model structure to be tested.

[0036] In the above technical scheme, the system of the present application simulates the functional area of ​​the brain through the impact area of ​​the cerebrospinal fluid impact simulation device, and places the model structure to be tested in the impact area; cerebrospinal fluid is supplied through the cerebrospinal fluid supply device, and the cerebrospinal fluid power regulating device controls and regulates the liquid flow and flow rate in the connecting pipeline. The liquid in the tube impacts the model structure to be tested located in the impact area at a set flow rate, and compares the cell number and differentiation index of the model structure to be tested before and after the impact, so as to realize the simulation of the impact of the flow direction and speed of cerebrospinal fluid on cells in the body and the analysis of the impact on cells.

[0037] As some optional embodiments, the cerebrospinal fluid supply device is provided with a fluid infusion port, to which a first valve 1.3 is connected, and the first valve controls the opening and closing of the fluid infusion port; the fluid infusion port is used to inject liquid into the cerebrospinal fluid supply device.

[0038] The cerebrospinal fluid supply device is provided with an exhaust port, to which a filter ventilation valve is connected; the filter ventilation valve controls the opening and closing of the exhaust port to filter pollutants in the atmosphere and the gas exchange of the cerebrospinal fluid supply device; the filter ventilation valve includes a filter unit and a valve, and the filter unit includes a core component filter element, and the filter unit is used to filter pollutants such as bacteria in the external atmosphere to avoid contamination of the liquid inside the cerebrospinal fluid supply device; the filter ventilation valve is opened periodically to exhaust the cerebrospinal fluid supply device and exchange gas; at the same time, when the cerebrospinal fluid supply device is replenished or injected with liquid, the filter ventilation valve is opened to exchange gas.

[0039] As an implementable embodiment, the cerebrospinal fluid supply device is a closed box structure, with a liquid inlet at one end of the box, a liquid outlet at the other end of the box, and a liquid inlet and an exhaust port at the top of the box.

[0040] Alternatively, the cerebrospinal fluid supply device 1 is a closed bottle structure, which includes a bottle body 1.1 and a bottle plug 1.2, the bottle plug closes the bottle mouth of the bottle body; the bottle plug is respectively provided with a liquid inlet hole and a liquid outlet hole, the liquid outlet hole is inserted with a liquid outlet tube 1.5, and the liquid outlet tube is connected to one end of the first pipeline; the liquid inlet hole is inserted with a liquid inlet tube 1.6, and the liquid inlet tube is connected to one end of the third pipeline; the bottle plug is respectively provided with a liquid infusion hole and a gas exhaust hole, the liquid infusion hole is inserted with a liquid infusion tube, and the first valve is arranged on the liquid infusion tube outside the bottle body; the gas exhaust hole is inserted with an gas exhaust pipe, and the filter ventilation valve 1.4 is arranged on the gas exhaust pipe outside the bottle body.

[0041] The first pipeline, the second pipeline and the third pipeline are preferably hoses, and the length and diameter of the hoses are set according to test requirements. In this embodiment, the inner diameter of the hose is 6.4 mm, and the outer diameter of the hose is 9.6 mm.

[0042] The box structure is preferably a transparent glass box, the bottle body is preferably a transparent glass bottle, and the bottle stopper is preferably an oak stopper. The transparent glass box and glass bottle are convenient for observing the internal liquid conditions. The cerebrospinal fluid power regulating device is an adjustable flow power pump device; the adjustable flow power pump device is equipped with a display, which is used to display the flow rate, rate and other parameters output by the power pump. The power pump provides 0-999ml / min flow regulation to meet the pressure and flow regulation requirements of medical experiments.

[0043] The cerebrospinal fluid impact simulation device includes a circular tube, and closed joints 3.2 are respectively provided at both ends of the circular tube, and the conducting ports of the closed joints at both ends of the circular tube are respectively connected to one end of the second pipeline and the third pipeline; an impact area 3.3 is provided in the circular tube, and the impact area is used to simulate the damaged part of the brain. Preferably, the impact area is a recessed structure set in the circular tube according to the recessed and / or raised structure of the functional area of ​​the brain; a model structure to be tested is placed in the impact area, and the model structure to be tested includes a model support and cells attached to the model support; the model support is printed by a 3D printing device simulation model. In this embodiment, the circular tube adopts a transparent glass circular tube 3.1, and the impact area adopts a groove (recessed structure) designed in the glass circular tube, and the transparent glass circular tube is convenient for observing the impact of the liquid. Open one of the closed joints, place the model structure to be tested in the impact area, the cerebrospinal fluid enters the cerebrospinal fluid impact simulation device through the second pipeline and then flows out through the third pipeline, the cerebrospinal fluid power regulating device controls and adjusts the liquid flow and flow rate in the connecting pipeline, the liquid in the tube impacts the structure model to be tested in the impact area at different flow rates, and compares the cell number and differentiation index of the structure model to be tested before and after the impact, so as to simulate the influence of the flow direction and speed of cerebrospinal fluid in the body on the cells and analyze the influence on the cells.

[0044] A flow meter and a pressure detector are provided on the second pipeline, and the flow meter and the pressure detector are used to check the flow rate and pressure on the second pipeline, and then correct the flow rate of the power pump controlling the cerebrospinal fluid power regulating device, and assist with the cerebrospinal fluid power regulating device to realize the different flow rate control output of the liquid in the pipeline; after the liquid with the controlled output flow rate flows through the impact area of ​​the glass round tube, the model bracket placed in the groove will be impacted by the fluid (liquid), and the number of cells attached to the bracket and the differentiation indicators before and after the impact will be compared; the influence of different impact sizes (different liquid flow rates and flow rates) on the cells on the bracket can be simulated and tested.

[0045] In this embodiment, a pressure reducing valve is provided on the third pipeline. After the impact, the liquid is reduced in pressure by the pressure reducing valve and then flows back to the cerebrospinal fluid supply device to prevent the liquid pressure flowing back to the cerebrospinal fluid supply device from being too high and damaging the cerebrospinal fluid supply device.

[0046] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. An extracorporeal simulated cerebrospinal fluid circulation system, characterized in that: It includes a cerebrospinal fluid supply device, a cerebrospinal fluid power regulating device, a cerebrospinal fluid impact simulation device and a connecting pipeline; the connecting pipeline includes a first pipeline, a second pipeline and a third pipeline; The cerebrospinal fluid supply device is provided with a liquid inlet and a liquid outlet; one end of the cerebrospinal fluid impact simulation device is provided with an input port, and the other end of the cerebrospinal fluid impact simulation device is provided with an output port; The liquid outlet is connected to the input end of the cerebrospinal fluid power regulating device through a first pipeline, the output end of the cerebrospinal fluid power regulating device is connected to the input port of the cerebrospinal fluid impact simulation device through a second pipeline, and the output port of the cerebrospinal fluid impact simulation device is connected to the liquid inlet of the cerebrospinal fluid supply device through a third pipeline; The cerebrospinal fluid power regulating device is used to control and regulate the flow rate and flow rate of the liquid in the second pipeline; The cerebrospinal fluid impact simulation device is provided with an impact area, and a model structure to be tested is placed in the impact area; the cerebrospinal fluid impact simulation device is used to realize that the model structure to be tested receives impact and to test the impact effect on the model structure to be tested; the cerebrospinal fluid supply device is provided with a fluid infusion port, and a first valve is connected to the fluid infusion port, and the first valve controls the opening and closing of the fluid infusion port; the fluid infusion port is used to inject liquid into the cerebrospinal fluid supply device; the cerebrospinal fluid supply device is provided with an exhaust port, and a filter ventilation valve is connected to the exhaust port; the filter ventilation valve controls the opening and closing of the exhaust port, and is used to filter pollutants in the atmosphere and gas exchange of the cerebrospinal fluid supply device; a flow meter and a pressure detector are provided on the second pipeline; a pressure reducing valve is provided on the third pipeline.

2. The cerebrospinal fluid extracorporeal simulated circulation system according to claim 1, characterized in that: The cerebrospinal fluid supply device is a closed box structure, with a liquid inlet at one end of the box, a liquid outlet at the other end of the box, and a liquid inlet and an exhaust port at the top of the box.

3. The cerebrospinal fluid extracorporeal simulated circulation system according to claim 1, characterized in that: The cerebrospinal fluid supply device is a closed bottle structure, which includes a bottle body and a bottle stopper, the bottle stopper seals the bottle mouth of the bottle body; the bottle stopper is respectively provided with a penetrating liquid inlet hole and a liquid outlet hole, the liquid outlet hole is inserted with a liquid outlet tube, and the liquid inlet hole is inserted with a liquid inlet tube; the bottle stopper is respectively provided with a penetrating liquid infusion hole and an exhaust hole, the liquid infusion hole is inserted with a liquid infusion tube, and the first valve is arranged on the liquid infusion tube outside the bottle body; the exhaust hole is inserted with an exhaust pipe, and the filter ventilation valve is arranged on the exhaust pipe outside the bottle body.

4. The cerebrospinal fluid extracorporeal simulated circulation system according to any one of claims 1 to 3, characterized in that: The cerebrospinal fluid power regulating device is an adjustable flow power pump device.

5. The in vitro simulated cerebrospinal fluid circulation system according to claim 1, characterized in that: The cerebrospinal fluid impact simulation device includes a circular tube, with closed joints at both ends of the circular tube respectively, and the conducting ports of the closed joints at both ends of the circular tube are respectively connected to the second pipeline and the third pipeline; an impact area is arranged in the circular tube, and the impact area is used to simulate the damaged part of the brain.

6. The in vitro simulated cerebrospinal fluid circulation system according to claim 5, characterized in that: The impact area is a concave structure arranged in the circular tube according to the concave and / or convex structure of the brain functional area.

7. The in vitro simulated cerebrospinal fluid circulation system according to claim 1, characterized in that: The model structure to be tested includes a model support and cells attached to the model support.

Images