Fully-automatic tissue section staining system and staining method

Abstract

The application discloses a kind of full-automatic tissue section staining system and staining method, it is related to pathological tissue section technical field.The staining system of it includes glass slide support, glass slide cover plate, scanning mobile platform, digital microscope.Several bottom visible reading sites are formed on glass slide support, each reading site is used to place glass slide;Glass slide cover plate is set corresponding to each reading site, to fit each glass slide to form the slit space containing tissue section.Scanning mobile platform is located below glass slide support, and can move along horizontal direction, digital microscope is carried on scanning mobile platform.The application can carry out dynamic monitoring to the degree of staining of each tissue section in color development, restaining step, stop staining in time when degree is appropriate, after completing staining, slice can be directly digitized scanning and stored as digital slice, allow to be carried out remote staining by remote, reading and call slice.

Description

Technical Field

[0001] This invention relates to the field of pathological tissue sectioning technology, specifically to a fully automated tissue sectioning staining system and staining method. Background Technology

[0002] Immunohistochemical staining is a widely used experimental technique in observing tissue morphology and conducting qualitative and quantitative analyses for clinical pathological diagnosis and experimental research. Currently, fully automated immunohistochemical staining instruments are widely used, but many procedures are relatively fixed, especially the development and counterstaining steps after staining. Technicians cannot observe and control the staining process in real time, and fixed procedures often lead to insufficient or excessive development or staining, which is detrimental to the observation and interpretation of slide results. After staining, slides still need to be manually mounted and then transferred to a slide scanner for scanning, which is labor-intensive and time-consuming, hindering large-scale experiments and rapid turnaround. With the development of telemedicine and the demands of the social environment, the medical field has an increasing need for remote operation. Current staining systems do not support remote control operation and remote monitoring of staining results, failing to meet the needs of remote staining and slide reading. Summary of the Invention

[0003] The purpose of this invention is to provide a fully automated tissue section staining system and method to solve the problems mentioned in the background art. This invention is convenient and quick to operate, and can dynamically monitor the staining degree of each tissue section in the color development and counterstaining steps. When the degree is appropriate, staining can be stopped in time. It also allows remote operation of the machine via network for remote staining, slide reading and slide retrieval.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A fully automated tissue section staining system includes:

[0006] A slide holder, wherein a plurality of bottom-visible reading positions are formed on the slide holder, and each reading position is used to place a slide;

[0007] A slide cover plate, wherein the slide cover plate is provided corresponding to each slide reading position, so as to fit each slide to form a narrow space for accommodating tissue sections;

[0008] A scanning moving platform is located below the slide holder and can move along the horizontal and vertical directions of the slide holder;

[0009] A digital microscope, which is mounted on the scanning moving platform;

[0010] The control module controls the movement of the scanning mobile platform and controls the digital camera to acquire tissue image information of each viewing position;

[0011] The display module acquires and displays tissue image information from the control module.

[0012] In one alternative embodiment, the slide cover plate is provided with a pure background light-emitting plate.

[0013] In one optional embodiment, the edge of the slide cover plate is provided with a sealing ring; the slide support is provided with a plurality of elastic hinges to hinge each slide cover plate to keep each slide cover plate in contact with the slide.

[0014] In one optional embodiment, each glass slide cover is provided with an electrothermal element and a temperature detector to achieve intracavitary heating of the slit space.

[0015] In one optional embodiment, the slide further includes a clamping device and a negative pressure suction device; each slide cover plate is provided with an inlet port, which is a one-way channel that allows only inflow and no outflow; the clamping device is provided with an electric pipette, which is equipped with multiple independently operable needles, which add reagents through the inlet port; each slide cover plate is provided with an outlet port, which allows only outflow and no inflow; the outlet port is connected to the negative pressure suction device via a drain pipe, and the outlet port is opened and closed by an electrically controlled valve.

[0016] In one optional embodiment, the device further includes a slide storage rack, a label reader horizontal moving block, a label reader laser, and a gripping device. The slide storage rack has multiple rows of slide placement positions. The label reader horizontal moving block can move to the position corresponding to each row of slide placement positions to encode the slides. The label reader horizontal moving block can move to each slide placement position to push out the slides. The gripping device is equipped with slide grippers and a barcode scanner. With the assistance of the barcode scanner and the label reader horizontal moving block, the slide grippers identify and grasp the slides.

[0017] In one optional implementation, each side of each slide placement position is provided with a set of indicator lights. One set of indicator lights indicates whether there is a slide in the current slide placement position by turning on and off, and the other set of indicator lights indicates whether the current slide has a code by turning on and off.

[0018] In one optional embodiment, the clamping device further includes an electric suction cup, which is used to adsorb the coverslip to complete the sealing of the slide, or to adsorb the cover plate of the slide to complete the opening of the cover.

[0019] In one optional embodiment, the microscope objectives in the digital microscope include a low-magnification microscope objective and a high-magnification microscope objective. The low-magnification microscope objective is used to acquire dynamic images of each slide, and the high-magnification microscope objective is used to realize digital scanning of the slides.

[0020] Another object of the present invention is to provide a staining method, which is implemented using the fully automated tissue section staining system as described above. The user controls the movement of the scanning mobile platform on-site or remotely, and controls the digital camera to acquire tissue image information at each reading position and display it on the display module.

[0021] By adopting the above technical solution, the present invention has the following advantages compared with the prior art:

[0022] This invention is convenient and quick to operate, and can dynamically monitor the staining degree of each tissue section in the color development and counterstaining steps. Staining can be stopped in time when the degree is appropriate. The system uses a clamping device to assist in operations such as changing the solution, transferring slides, and mounting slides. After staining is completed, the slides can be directly digitized and stored as digital slides. The machine can be remotely operated via network for remote staining, slide reading, and slide retrieval. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a three-dimensional view of the system structure of the present invention;

[0025] Figure 2 This is a three-dimensional structural schematic diagram of the slide support of the present invention;

[0026] Figure 3 This is a schematic diagram of the side structure of the slide support of the present invention;

[0027] Figure 4 This is a schematic diagram of the clamping device of the present invention;

[0028] Figure 5 This is a side view schematic diagram of the glass slide storage rack of the present invention;

[0029] Figure 6 This is a three-dimensional structural diagram of the glass slide storage rack of the present invention.

[0030] Explanation of reference numerals in the attached figures:

[0031] 1. Slide; 2. Tissue; 3. Slide support; 31. Flexible hinge; 32. Support electrical signal interface; 33. Reading position;

[0032] 4. Slide cover plate, 41. Liquid inlet port, 42. Sealing ring, 43. Heating glass electrode, 44. Heating glass, 45. Liquid outlet port, 451. Drain pipe, 46. Electrically controlled valve, 47. Valve wire, 48. Heating glass wire, 49. Temperature detector.

[0033] 5. Low-magnification microscope objective; 6. High-magnification microscope objective; 7. Digital camera; 8. Scanning moving platform; 9. Vertical guide rail; 10. Horizontal guide rail; 11. Cover plate support for slide.

[0034] Clamping device 12, clamping device data and fluid interface 121, clamping device base 122, clamping device turntable 123, clamping device first joint 124, clamping device second joint 125, clamping device third joint 126;

[0035] The following components are included: gripping device extension head 127, barcode scanning and identification device 1271, electric pipette 1272, electric suction cup 1273, slide gripper 1274, slide storage rack 13, slide placement position 131, label reader longitudinal moving stage 14, label reader laser 15, label reader longitudinal guide rail 16, label reader horizontal moving block 17, label reader horizontal guide rail 18, and slide indicator light 19.

[0036] Control module 20, display module 21. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0038] In the description of this invention, it should be understood that the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0039] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0040] Example

[0041] See Figures 1-6 As shown, the present invention discloses a fully automated tissue section staining system, which includes a slide holder 3, a slide cover plate 4, a scanning moving platform 8, a digital microscope, a control module 20, and a display module 21.

[0042] The slide holder 3 has several bottom-visible reading positions, each used to place a slide 1. In this embodiment, the slide holder 3 can be set as a transparent base plate, so that each reading position is visible from the bottom.

[0043] The scanning platform 8 is located below the slide holder 3 and can move along the horizontal and vertical directions of the slide holder 3. The digital microscope is mounted on the scanning platform 8. The microscope objectives in the digital microscope include a low-magnification microscope objective 5 and a high-magnification microscope objective 6. The low-magnification microscope objective 5 is used to acquire dynamic images of each slide 1, and the high-magnification microscope objective 6 is used to realize digital scanning of the slides.

[0044] In this way, the user can control the scanning mobile platform 8 to move on-site or remotely via the control module 20, and control the digital camera 7 to acquire tissue image information from each viewing position. The display module 21 acquires and displays the tissue image information from the control module 20.

[0045] A slide cover plate 4 is provided corresponding to each reading position to form a narrow slit space for accommodating tissue sections by fitting each slide. In this embodiment, the slide cover plate 4 is connected to the slide holder 3 via a slide cover plate support shaft 11, and the slide holder 3 has an elastic hinge 31 to maintain a tight fit between the slide cover plate 4 and the slide 1. A sealing ring 42 is provided at the edge of the slide cover plate 4 to maintain airtightness.

[0046] The slide cover plate 4 has an inlet port 41 and an outlet port 45. To facilitate liquid entry and exit, the inlet port 41 is a one-way channel, allowing only liquid to enter and not exit; similarly, the outlet port 45 allows only liquid to exit and not enter. The outlet port 45 is connected to a drain pipe 451, which is connected to a negative pressure suction device (not shown in the figure). The drainage from the outlet port 45 is controlled by an electrically controlled valve 46. Thus, by opening and closing the inlet port 41, the outlet port 45, and the electrically controlled valve 46, operations such as reagent filling or liquid replacement within the narrow space can be achieved.

[0047] In this invention, the slide cover plate 4 is provided with a pure background light-emitting plate to emit light and assist in image observation. In this application, the pure background light-emitting plate is a white light-emitting plate.

[0048] In this invention, the slide cover plate 4 is equipped with an electrothermal element (electrothermal glass electrode 43, electrothermal glass 44) and a temperature detector 49 to achieve intracavitary heating of the slit space and provide heating assistance for the staining method.

[0049] Specifically, the electrothermal glass 44 and the electrothermal glass electrode 43 form an electrothermal element to achieve intracavitary heating in the slit space. The temperature detector 49 is used to detect the intracavitary temperature and provide feedback to the control module 20 for temperature control. The electrically controlled valve 46, the electrothermal glass electrode 43, and the temperature detector 49 are connected to the support electrical signal interface 32 on the slide support 3 via valve wire 47 and electrothermal glass wire 48, and are subject to control by the control module 20.

[0050] The present invention also includes a clamping device 12, which has a clamping device base 122. The clamping device base 122 contains a liquid addition drive device (not shown in the figure), a negative pressure suction device (not shown in the figure), and a clamping device data and liquid circuit interface 121.

[0051] The gripping device base 122 is electrically connected to the control module 20, and the liquid-filling drive device provides the mechanical power required by the gripping device 12. The gripping device turntable 123 allows the gripping device 12 to rotate 360° on the base. Each of the gripping device's first joint 124, second joint 125, and third joint 126 has a servo motor (not shown in the figure), which can perform large-amplitude rotations to complete various actions.

[0052] The gripping device extension head 127 is equipped with a barcode scanner 1271, which is responsible for image recognition and barcode scanning confirmation to assist in the positioning of the gripping device 12.

[0053] The electric pipette 1272 has different needles that can work independently. When not in use, the needles can be extended and retracted into the extension head 127.

[0054] The electric suction cup 1273 is connected to the negative pressure suction device of the clamping device base 122, which can pick up the cover glass slide for sealing operation, or it can pick up the cover glass slide to open the cover.

[0055] In a preferred embodiment, the present invention further includes a slide storage rack 13, a label reader horizontal moving block 17, and a label reader laser 15. The slide storage rack 13 has multiple rows of slide placement positions, and the label reader laser 15 can be moved to the position corresponding to each row of slide placement positions to encode the slides 1. The label reader horizontal moving block 17 can be moved to each slide placement position to eject the slides.

[0056] With the assistance of the barcode scanner 1271 and the label horizontal moving block 17, the slide gripper 1274 identifies and picks up the slide 1.

[0057] Each slide placement position 131 has a set of indicator lights 19 on both sides. The left set of indicator lights indicates whether there is a slide in the current slide placement position by turning it on and off, and the other set of indicator lights (right set of indicator lights) indicates whether the current slide has a code by turning it on and off (the turning of the indicator lights is completed by the control module recording the movement of the laser and the label reader horizontal moving block).

[0058] Specifically, the label laser 15 is located on the top side and is combined with the label longitudinal moving stage 14. The two can slide along the label longitudinal guide rail 16 to align with different columns of the slide storage rack 13. Below the slide storage rack 13 are the label horizontal guide rail 18 and the label horizontal moving block 17. The label horizontal moving block 17 moves along the label horizontal guide rail 18 to reach different slide placement positions 131 of each column of slides 1. When it reaches the target slide 1, the label horizontal moving block 17 can extend and retract in the longitudinal direction (this application does not specifically limit the label horizontal moving block 17, it can be a miniature electric push rod) to push the slide 1 upward. The label laser 15 prints the coding information in the label area of ​​the slide 1. After coding is completed, the indicator light on the right side of the slide placement position 131 lights up.

[0059] Example 2

[0060] This invention also discloses a staining method, which is implemented using the fully automated tissue section staining system described above. The user controls the scanning mobile platform to move on-site or remotely, and controls the digital camera 7 to acquire tissue image information at each reading position, which is then displayed on the display module 21. This allows for dynamic monitoring of the staining degree of each tissue section during the color development and counterstaining steps, and the staining can be stopped in time when the degree is appropriate. The system uses a clamping device 12 to assist in operations such as changing the solution, transferring slides, and mounting slides. After staining is completed, the slides can be directly digitized and stored as digital slides. The system allows for remote operation of the machine via network for remote staining, slide reading, and slide retrieval.

[0061] Specifically, it includes the following steps:

[0062] 1. Slide loading and coding;

[0063] At the start of the experiment, the slide 1 of the tissue 2 to be stained is placed on the slide storage rack 13. The slide storage rack 13 has multiple slide placement positions 131. There are indicator lights 19 on both sides of the slide placement positions 131. After the slide 1 is placed, the indicator light 19 on the left side lights up, indicating that there is a slide 1 at that position. After the slide 1 is placed, the information of each slide 1, including the ID number and staining index, is entered into the control module 20. The control module 20 simultaneously assigns the position of each slide 1 to the slide holder 3.

[0064] The labeler horizontal moving block 17 moves along the labeler horizontal guide rail 18, reaches below the target slide 1, pushes the slide 1 upward, and the labeler laser 15 prints the coding information in the label area of ​​the slide 1. After coding is completed, the indicator light 19 on the right side of the slide placement position 131 lights up, indicating that coding is complete.

[0065] 2. Transfer the slide to the mounting plate;

[0066] Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 As shown, after the encoding is completed, the gripping device 12, with the assistance of the barcode scanner 1271, sequentially removes each slide 1. The label horizontal moving block 17 sequentially pushes out the slide 1 in the vertical direction. The gripping device 12 completes the removal action. The gripping device 12 places the slide 1 on the reading position 33 of the slide holder 3, and covers it with the slide cover plate 4, forming a narrow space with the slide 1. The tissue 2 on the slide 1 is located in this narrow space.

[0067] 3. Baking slices;

[0068] The slide cover plate 4 is heated by the electric heating glass 44 to heat the tissue 2 on the slide 1. The temperature is fed back to the control module 20 by the temperature detector 49 for setting.

[0069] 4. Dewaxing and hydration;

[0070] After the slide is baked, the control module 20 controls the gripping device 12 to draw up a sufficient amount of dewaxing reagent through the electric pipette 1272. Appropriate amounts of reagent are added to the narrow space between the slide cover plate 4 and the slide 1 through the liquid inlet port 41 to dewax the tissue 2. The electric heating glass 44 provides moderate heating to assist dewaxing. After dewaxing, the reagent is discharged from the liquid outlet port 45 through the drainage pipe. The drainage operation is controlled by the control module 20 through the electric valve 46. Hydration reagents are added sequentially until the tissue is completely hydrated. The tissue 2 is immersed in PBS solution (phosphate buffer solution). The gripping device 12 is automatically cleaned before drawing up different reagents through the electric pipette 1272.

[0071] 5. Antigen retrieval;

[0072] The control module 20 controls the clamping device 12 to draw up a sufficient amount of antigen retrieval solution through the electric pipette 1272 and add it to the glass slide cover plate 4 through the inlet port 41. Different antigen retrieval solutions can be added according to different antibody requirements. The electric heating glass 44 heats the antigen retrieval process, and the temperature detector 49 records the temperature. After the retrieval is completed, the solution is allowed to cool naturally, and the reagent is discharged from the outlet port 45. PBS solution is added again for soaking and rinsing before being discharged.

[0073] 6. Enclosed;

[0074] The control module 20 controls the clamping device 12 to draw up a sufficient amount of sealing reagent through the electric pipette 1272, and adds the glass slide cover plate 4 through the liquid inlet port 41. The control module 20 times the sealing time. After sealing is completed, the reagent is discharged from the liquid outlet port 45, and PBS solution is added again for rinsing and soaking.

[0075] 7. Change the medium and incubate the antibody;

[0076] The control module 20 controls the gripping device 12 to sequentially draw different primary antibody reagents through the electric pipette 1272 and add them into the slide cover plate 4 through the liquid inlet port 41. Before the antibody is added, the PBS solution on each slide 1 is fully drained. After the antibody reagent is added, the control module 20 automatically starts timing. After the timing is completed, the gripping device 12 sequentially performs operations such as changing the solution, rinsing, and adding secondary antibody. After the antibody incubation is completed and the tissue is rinsed, the tissue 2 is immersed in PBS solution.

[0077] 8. Color development;

[0078] The control module 20 controls the clamping device 12 to prepare and aspirate a sufficient amount of colorimetric solution via the electric pipette 1272. At the same time, another suction needle aspirates a sufficient amount of double-distilled water as a backup for stopping the colorimetric process. The colorimetric solution is added into the slide cover plate 4 through the inlet port 41. Before adding the colorimetric solution, the PBS solution on each slide 1 is fully drained. After the colorimetric reagent is added, the control module 20 automatically starts timing.

[0079] The scanning platform 8 located below the slide holder 3 can move freely and focus. When the staining process reaches the color development stage and the color development solution is added, the scanning platform 8 can be controlled by the control module 20 to see the real-time color development image of tissue 2 captured by the digital camera 7 on the display module 21. The slide cover plate 4 can emit light and serve as a white background to assist in image observation. When the color development is appropriate, the reaction can be stopped in time and confirmed. This step is usually completed under the low magnification microscope objective 5. The counterstaining process is carried out in the same way.

[0080] Below the slide holder 3 is a scanning moving platform 8. The low-magnification microscope objective 5, the high-magnification microscope objective 6, and the digital camera 7 constitute the digital camera 7 and are mounted on the movable platform 8. The scanning moving platform 8 is provided with a horizontal guide rail 10 and a vertical guide rail 9. The scanning moving platform 8 can move on the horizontal guide rail 10 and make slight movements on the vertical guide rail 9. When the color developing solution is added, the control module 20 obtains dynamic images of each slide 1 by controlling the scanning moving platform 8 and displays them on the display module 21 or transmits them remotely to a remote user. The control module 20 can automatically identify the color development degree. When the color development is appropriate, the color developing solution is discharged and sufficient double-distilled water is added through the clamping device 12 to stop the color development. The color development can also be manually stopped by the operator on-site or under remote monitoring. After the color development is completed, the clamping device 12 automatically performs several liquid replacements and rinses, and the tissue 2 is immersed in double-distilled water.

[0081] 9. Restaining;

[0082] The re-dyeing process is carried out in the same way, and finally tissue 2 is immersed in double-distilled water in the VB program.

[0083] 10. Dry tablets;

[0084] After all staining reactions are completed, double-distilled water is discharged from the outlet port 45. The electrically controlled valve 46 remains open for a period of time. Under the drive of the negative pressure suction device, air continuously enters from the inlet port 41 to form a laminar flow. The electric heating glass 44 of the slide cover plate 4 assists in heating to dry the slide 1 of tissue 2. After drying, the clamping device 12 opens the slide cover plate 4 to prepare for sealing.

[0085] 11. Sealing;

[0086] The control module 20 uses the electric pipette 1272 of the clamping device 12 to draw up a sufficient amount of sealing liquid, and with the assistance of the barcode scanner 1271, it is dropped onto each dried tissue 2. The electric suction cup 1273 then picks up the liquid and seals the tissue.

[0087] 12. Digital scanning;

[0088] After all operations are completed, the slide 1 is placed on the slide holder 3. The digital camera 7 switches to the high-magnification microscope objective 6 and performs a digital scan of the slide 1 with the assistance of the mobile platform 8. The image data is automatically named according to the code, stored in the control module 20 and displayed on the display module 21. The control module 20 is connected to the Internet and can automatically transmit the data to the cloud and the doctor's workstation. After the mounting fluid has dried completely, the clamping device 12 automatically puts the slide 1 back into the slide storage rack 13, or keeps the slide 1 in the slide holder 3.

[0089] 13. Remote control and film viewing;

[0090] After the control module 20 is connected to the Internet, remote users can remotely operate the control module 20 to complete the above staining operation process and check the progress at any time. After the slide 1 is digitized and scanned, remote users can view the digital image on the display module 21. After staining, the slide 1 is still located on the slide holder 3. Remote users can control the scanning moving platform 8 to read the slide. If the slide 1 has been placed in the slide storage rack 13, remote users can use the clamping device 12 to retrieve the corresponding slide 1 and place it back on the slide holder 3 for remote reading.

[0091] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0092] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A fully automated tissue section staining system, characterized in that, include: A slide holder, wherein a plurality of bottom-visible reading positions are formed on the slide holder, and each reading position is used to place a slide; The slide cover plate is provided corresponding to each reading position to fit each slide to form a narrow space for accommodating tissue sections; each slide cover plate is provided with a liquid inlet port, which is a one-way channel that allows liquid to enter but not exit; each slide cover plate is provided with a liquid outlet port, which allows liquid to exit but not enter, and the liquid outlet port is opened and closed by an electrically controlled valve. A scanning moving platform is located below the slide holder and can move along the horizontal and vertical directions of the slide holder; A digital microscope, which is mounted on the scanning moving platform; The control module controls the movement of the scanning platform and controls the digital microscope to acquire tissue image information from each viewing position. The display module acquires and displays tissue image information from the control module. A clamping device is provided, which is equipped with an electric pipette, and the electric pipette is provided with multiple independently working needles, which add reagents through the inlet port; A negative pressure suction device, wherein the liquid outlet is connected to the negative pressure suction device via a drain pipe; It also includes a slide storage rack, a label horizontal moving block, and a label laser; the slide storage rack is provided with multiple rows of slide placement positions, and the label horizontal moving block can move to the position corresponding to each row of slide placement positions to encode the slides; the label horizontal moving block can move to each slide placement position to push out the slides; the gripping device is also provided with slide grippers and a barcode scanner, and the slide grippers, with the assistance of the barcode scanner and the label horizontal moving block, identify and grip the slides.

2. The fully automated tissue section staining system as described in claim 1, characterized in that: The glass slide cover plate is equipped with a pure background light-emitting plate.

3. The fully automated tissue section staining system as described in claim 1, characterized in that: The edge of the slide cover plate is provided with a sealing ring; the slide support is provided with several elastic hinges to hinge each slide cover plate to keep each slide cover plate in contact with the slide.

4. The fully automated tissue section staining system as described in claim 1, characterized in that: Each glass slide cover is equipped with an electric heating element and a temperature detector to achieve intracavitary heating of the slit space.

5. The fully automated tissue section staining system as described in claim 1, characterized in that: Each slide placement position has a set of indicator lights on both sides. One set of indicator lights indicates whether there is a slide in the current slide placement position by turning it on and off, and the other set of indicator lights indicates whether the current slide has a code by turning it on and off.

6. The fully automated tissue section staining system as described in claim 1, characterized in that: The clamping device also includes an electric suction cup, which is used to adsorb the cover glass to complete the sealing of the slide, or to adsorb the cover plate of the slide to complete the opening of the cover.

7. The fully automated tissue section staining system as described in claim 1, characterized in that: The digital microscope includes low-magnification and high-magnification microscope objectives. The low-magnification microscope objectives are used to acquire dynamic images of each slide, and the high-magnification microscope objectives are used to realize digital scanning of slides.

8. A staining method, characterized in that: The fully automated tissue section staining system as described in claim 1 is used to achieve this. The user controls the scanning mobile platform to move on-site or remotely, and controls the digital microscope to acquire tissue image information from each reading position, which is then displayed on the display module.

Images