An in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform

Abstract

The invention relates to the field of equipment for instrumental analysis, and discloses an in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform, comprising an upper base, a lower base, a xenon lamp, an optical fiber spectrometer, a photocell sensor, and a control circuit. The upper base and the lower base are arranged opposite to each other, one side of the lower base is rotatably connected to one side of the upper base, an in-situ droplet seat is arranged on the other side of the lower base, an upper optical fiber seat corresponding to the position of the in-situ droplet seat is arranged on the upper base, an LED seat is arranged on the lower base, the LED seat comprises a fluorescent LED module and a turbidity LED module, a focusing collimating lens is arranged on the upper base, light emitted by the fluorescent LED module and the turbidity LED module is irradiated to the in-situ droplet seat through the focusing collimating lens, the xenon lamp is connected to the upper optical fiber seat of the upper base through an optical fiber, and the optical fiber spectrometer and the photocell sensor are connected to the in-situ droplet seat through an optical fiber. The invention realizes multi-purpose of one machine, improves sample testing efficiency, and reduces sample waste.

Description

Technical Field

[0001] The invention relates to the field of instrumental analysis equipment, in particular to an in-situ ultra-micro volume, fluorescence and turbidity multifunctional droplet measurement platform. Background Art

[0002] Ultra-micro spectrophotometer can quickly and accurately detect nucleic acids, proteins and cell solutions. It is easy to use, consumes less sample (only 0.5-2μl), does not need to be preheated, can quickly clean up residual samples, does not require cuvettes or other sample positioning devices, and does not require sample dilution. Fluorescence spectrophotometer is a highly sensitive quantitative analysis method that accurately measures DNA, RNA and protein. It has good sensitivity and specificity and is used in the detection of exogenous DNA residues in recombinant products, the detection of drug residual DNA, and the detection of free DNA content. Bacterial cell density (OD600) in turbidity meters is an important application. It refers to the absorbance of a solution at a wavelength of 600nm. The absorption of bacteria is used to measure the concentration of bacterial culture fluid, thereby estimating the growth of bacteria.

[0003] Existing ultra-micro spectrophotometers, fluorescence photometers and turbidity meters are independent devices. It is impossible to achieve multiple uses for one machine and perform in-situ measurements. For laboratories, they not only take up space, but also cannot be switched freely. Different instrument tests require frequent sample transfers, which easily causes sample waste and contamination. In addition, switching tests between multiple instruments requires more time, which greatly reduces the test efficiency of the samples. Summary of the invention

[0004] Therefore, it is necessary to provide an in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform to solve the problem of low test efficiency and sample waste caused by the need to use different instruments for multiple sample tests.

[0005] To achieve the above-mentioned purpose, the present invention provides an in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform, comprising an upper base, a lower base, a xenon lamp, a fiber optic spectrometer, and a photocell sensor, wherein the upper base and the lower base are arranged opposite to each other, one side of the lower base is rotatably connected to one side of the upper base, an in-situ droplet seat is arranged on the other side of the lower base, an upper fiber optic seat corresponding to the position of the in-situ droplet seat is arranged on the upper base, an LED seat is arranged on the lower base, the LED seat comprises a fluorescent LED module and a turbidity LED module, the light emitted by the fluorescent LED module and the turbidity LED module irradiates the in-situ droplet seat, the xenon lamp is connected to the upper fiber optic seat of the upper base through an optical fiber, and the fiber optic spectrometer and the photocell sensor are connected to the in-situ droplet seat through an optical fiber,

[0006] It also includes a control circuit, which includes a processor U1, a two-way LED constant current power supply circuit, a two-color LED circuit, a PD sampling circuit and an optical switch module circuit. The processor U1 is electrically connected to the fiber optic spectrometer, the xenon lamp, the optical switch module, the PD sampling circuit and the two-way LED constant current power supply circuit respectively. The two-way LED constant current power supply circuit is connected to the two-color LED circuit respectively. The two-color LED circuit includes the fluorescent LED module and the turbidity LED module. The PD sampling circuit includes an analog-to-digital conversion circuit and a power amplifier circuit. The photocell sensor includes a photocell PD1. The photocell PD1 is connected to the processor U1 through a power amplifier circuit and an analog-to-digital conversion circuit.

[0007] Furthermore, the two LED constant current power supply circuits are respectively a first constant current power supply circuit and a second constant current power supply circuit. The first constant current power supply circuit includes a capacitor C8, a capacitor C4, a resistor R3, a resistor R2, an inductor L1, a capacitor C1, a resistor R1, a capacitor C13, an LED driver U3, a capacitor C10, a capacitor C9 and a 5V DC power supply.

[0008] Pin 19 of the processor U1 is connected to resistor R1, and the other end of resistor R1 is connected to pin 5 of the LED driver U3. A 5V DC power supply is connected to capacitor C9, capacitor C10 and pin 3 of the LED driver U3. The other ends of capacitor C9 and capacitor C10 are grounded. Pin 1 of the LED driver U3 is grounded. Pin 4 of the LED driver U3 is connected to capacitor C13 and resistor R2. The other end of capacitor C13 is grounded. The other end of resistor R2 is connected to resistor R3 and a common end. The other end of resistor R3 is grounded. Pin 6 of the LED driver U3 is connected to capacitor C1. The other end of capacitor C1 is connected to pin 2 of the LED driver U3 and inductor L1. The other end of inductor L1 is connected to the output end. Both ends of capacitor C8 and capacitor C4 are connected to the output common end respectively.

[0009] The connection structure of the second constant current power supply circuit is the same as that of the first constant current power supply circuit. The second constant current power supply circuit is connected to the pin 20 of the processor U1.

[0010] The first constant current power supply circuit and the second constant current power supply circuit are electrically connected to the dual-color LED circuit through the connector J2 and the connector J3, and the connector J2 and the connector J3 are a pair of male and female connection ports.

[0011] Furthermore, the model of the LED driver U3 is TPS54201, the value of the capacitor C4 is 10 μF, the value of the capacitor C8 is 100 nF, the value of the inductor L1 is 68 μH, the value of the resistor R2 is 510R, and the value of the resistor R1 is OR.

[0012] Furthermore, the optical switch module circuit includes resistor R7, resistor R8, resistor R6, switch driver U5, capacitor C26, pin 17 of processor U1 is connected to resistor R7, the other end of resistor R7 is connected to resistor R6 and pin 1 of switch driver U5, the other end of resistor R6 is grounded, pin 2 of switch driver U5 is grounded, pin 18 of processor U1 is connected to resistor R8, the other end of resistor R7 is connected to pin 3 of switch driver U5, pin 5 of switch driver U5 is connected to +5V power supply and capacitor C26, the other end of capacitor C26 is grounded, pin 4 and pin 6 of switch driver U5 are respectively connected to M2 end and M1 end, and the M2 end and M1 end are optical switches of the optical fiber spectrometer, and the optical switch of the optical fiber spectrometer is used to control the switching of the filter.

[0013] Furthermore, the model of the switch driver U5 is AP1511A, the values ​​of the resistors R7 and R8 are 0R, the value of the resistor R6 is 10K, and the specification of the capacitor C26 is 1UF / 50V.

[0014] Further, the analog-to-digital conversion circuit includes an analog-to-digital converter U6, a resistor R13, a resistor R12, a capacitor C29, a capacitor C30 and a resistor R21, pins 26 and 27 of the processor U1 are respectively connected to pins 5 and 6 of the analog-to-digital converter U6, one end of the resistor R13 and the resistor R12 are respectively connected to pins 5 and 6 of the analog-to-digital converter U6, the other ends of the resistors R13 and R12 are grounded, pin 7 of the analog-to-digital converter U6 is connected to a +5V power supply, one end of the capacitors C29 and C30 are connected to pin 7 of the analog-to-digital converter U6, the other ends of the capacitors C29 and C30 are grounded, pin 8 of the analog-to-digital converter U6 is connected to pin 1 of the analog-to-digital converter U6, pin 2 of the analog-to-digital converter U6 is grounded, pin 3 of the analog-to-digital converter U6 is connected to resistor R21, and the other end of the resistor R21 is grounded.

[0015] The power amplifier circuit includes resistor R18, resistor R17, capacitor C35, operational amplifier U7B, resistor R16, operational amplifier U7A, resistor R20, resistor R14, capacitor C32, capacitor C33, resistor R19, capacitor C31, capacitor C28, resistor R11 and capacitor C22, pin 4 of operational amplifier U7A is grounded, pin 3 of operational amplifier U7A is connected to resistor R20, the other end of resistor R20 is grounded, pin 2 of operational amplifier U7A is connected to resistor R14 and capacitor C32, the other ends of resistor R14 and capacitor C32 are connected to the positive electrode of photocell PD1, one end of resistor R19 is connected to the positive electrode of photocell PD1, the other end of resistor R19 is grounded, the negative electrode of photocell PD1 is connected to +5V power supply, one end of capacitor C33 is connected to 5V power supply, the other end of capacitor C33 is grounded, pin 8 of operational amplifier U7A is connected to 5V power supply, capacitor C31 and capacitor C28, The other ends of capacitor C31 and capacitor C28 are grounded, pin 1 of operational amplifier U7A is connected to resistor R16, resistor R11 and resistor R9, the other end of resistor R9 is connected to capacitor C22, the other ends of resistor R11 and capacitor C22 are connected to pin 2 of operational amplifier U7A, the other end of resistor R16 is connected to pin 5 of operational amplifier U7B, pin 6 of operational amplifier U7B is grounded, pin 7 of operational amplifier U7B is connected to resistor R17, the other end of resistor R17 is grounded and connected to resistor R18, and the other end of resistor R18 is connected to pin 4 of analog-to-digital converter U6.

[0016] Furthermore, the models of the operational amplifier U7B and the operational amplifier U7A are OPA2350, and the model of the analog-to-digital converter U6 is CS1237.

[0017] Furthermore, the PD sampling circuit further includes a capacitor C34 and a test point TP1 , both of which are connected to the resistor R18 and the pin 4 of the analog-to-digital converter U6 , and the other end of the capacitor C34 is grounded.

[0018] Furthermore, the control circuit also includes a connector J4 and a connector J1, pins 12 and 13 of the processor U1 are connected to the xenon lamp through the connector J4, and pins 30 and 31 of the processor U1 communicate with the spectrometer through the connector J1. The model of the processor U1 is STM32F030.

[0019] Furthermore, the fluorescent LED module includes a blue LED light source, a collimator and a focusing lens, and the turbidity LED module includes a yellow LED light source and a collimator. The light emitted by the blue LED light source is irradiated onto the sample of the in-situ droplet seat through the collimator and the focusing lens, and the light emitted by the yellow LED light source is irradiated onto the sample of the in-situ droplet seat through the collimator.

[0020] The above technical solution has the following beneficial effects:

[0021] In the present invention, the processor U1 serves as the overall control center to control the operation of the entire device. The two-way LED constant current power supply circuit can provide a stable light source for the LED holder including the fluorescent LED module and the turbidity LED module to ensure that the LED is in the best working state. The optical switch module circuit cooperates to select which LED emits light to irradiate the sample, which is then received by the PD, and then preliminarily processed by the PD sampling circuit and sent to the processor. The processor performs analysis and processing based on the data obtained by the PD. The other part is controlled by the processor to emit light from the xenon lamp, which is transmitted through the optical fiber and then irradiated onto the sample, and then received by the optical fiber into the spectrometer. The spectrometer analyzes and processes the light, and then transmits the data to the processor for further analysis and processing.

[0022] Working principle of LED constant current source circuit and PD circuit: The processor outputs pulse width to control the LED driver U3-TPS54201 to output the required stable current to drive the LED, so that the LED emits stable light. After passing through the tested sample, there will be a slight change that is collected by the PD end, and then the power amplifier circuit amplifies this slight change, and then sends it to the analog-to-digital converter U6 of the analog-to-digital conversion circuit, which is converted into a digital signal and sent to the processor

[0023] The working principle of the optical switch module circuit: The optical switch module is driven by the processor, and M1 and M2 of the optical switch module are directly connected to the heating optical switch of the fiber optic spectrometer. The optical switch is equipped with a filter, which is turned on when the filter is needed and turned off when the filter is not needed. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a front view of the measurement platform described in the specific implementation method.

[0025] Figure 2 It is a cross-sectional view of the measurement platform described in the specific implementation manner.

[0026] Figure 3 It is an exploded view of the upper base and the lower base described in the specific implementation method.

[0027] Figure 4 It is a three-dimensional diagram of the lower base described in the specific implementation method.

[0028] Figure 5 It is a circuit connection diagram of the processor U1 described in the specific implementation manner.

[0029] Figure 6 It is a connection diagram of the two-way LED constant current power supply circuit described in the specific implementation method.

[0030] Figure 7 It is a connection diagram of the optical switch module circuit described in the specific implementation method.

[0031] Figure 8 It is a connection diagram of the PD sampling circuit described in the specific implementation method.

[0032] Fig. 9 It is a connection diagram of the power amplifier circuit described in the specific implementation method.

[0033] Fig.10 It is a connection diagram of the analog-to-digital conversion circuit described in the specific implementation method.

[0034] Fig.11 It is a connection diagram of the voltage stabilizing circuit described in the specific implementation method.

[0035] Description of reference numerals:

[0036] 1. Upper base; 2. Lower base; 3. Upper shell; 4. Lower shell; 5. In-situ droplet seat; 6. Positioning assembly; 61. Positioning plate; 62. Positioning column; 63. Positioning hole; 7. Xenon lamp; 8. Fiber optic spectrometer; 9. Photocell sensor; 10. Upper fiber optic seat; 11. LED seat; 111. Fluorescent LED module; 112. Turbidity LED module; 12. Fluorescent lifting motor; 13. Rotating support seat; 14. Rotating shaft; 15. Connecting hole; 16. Support boss; 17. Turbidity lifting motor; 18. Vertical rod; 19. Support rod. DETAILED DESCRIPTION

[0037] In order to explain the technical content, structural features, achieved objectives and effects of the technical solution in detail, the following is a detailed description in conjunction with specific embodiments and accompanying drawings.

[0038] See also Figure 1-11 The present embodiment provides an in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform, comprising an upper base 1, a lower base 2, a xenon lamp 7, an optical fiber spectrometer 8, a photocell sensor 9, a positioning assembly 6, and a control circuit. The upper base 1 and the lower base 2 are arranged opposite to each other, one side of the lower base 2 is rotatably connected to one side of the upper base 1, and the other side of the lower base 2 is provided with an in-situ droplet seat 5. The upper base 1 is provided with an upper optical fiber seat 10 corresponding to the position of the in-situ droplet seat 5, and the lower base 2 is provided with an LED seat 11. The LED seat 11 includes a fluorescent LED module 111 and a turbidity LED module 112. The light emitted by the fluorescent LED module 111 and the turbidity LED module 112 irradiates the in-situ droplet seat 5. The xenon lamp 7 is connected to the upper optical fiber seat 10 of the upper base 1 through an optical fiber, and the optical fiber spectrometer 8 and the photocell sensor 9 are connected to the in-situ droplet seat 5 through an optical fiber. In this embodiment, the optical fiber of the lower base 2 is a two-in-one optical fiber, which is respectively connected to the optical fiber spectrometer 8 and the photocell sensor 9.

[0039] The upper base 1 is provided with a sealed upper shell 3, the lower base 2 is provided with a sealed lower shell 4, the xenon lamp 7, the optical fiber spectrometer 8, and the photocell sensor are fixed in the lower shell 4, and the upper base 1 is provided with a connecting hole 15, and the optical fiber connected to the xenon lamp 7 passes through the connecting through hole to connect with the upper optical fiber holder 10. The sealed upper shell 3 and the lower shell 4 can facilitate the test of the obtained optical signal and avoid the influence of the external environment. At the same time, the heavy xenon lamp 7 and the optical fiber spectrometer 8 are arranged in the lower shell 4 to stabilize the center of gravity of the device.

[0040] The optical fiber spectrometer 8 is located directly below the in-situ droplet seat 5, and a fluorescent filter is provided on the optical fiber spectrometer 8. The photocell sensor 9 is located on the side below the in-situ droplet seat 5. The photocell sensor 9 receives scattered light of the sample droplet at a horizontal angle of 90° below the in-situ droplet seat 5.

[0041] The positioning device includes a positioning plate 61 and a positioning column 62. The positioning plate 61 is fixed on the lower base 2. The positioning plate 61 is provided with a positioning hole 63. The positioning column 62 is fixed on the bottom surface of the upper base 1. The positioning column 62 and the positioning hole 63 are located in a corresponding position. The upper base 1 and one side of the lower base 2 are relatively rotated. After the sample droplets are dripped on the in-situ droplet holder 5, the upper base 1 is closed. The positioning column 62 cooperates with the positioning hole 63 to limit the position of the upper base 1 after the plate is flipped and closed, which is conducive to the alignment of the upper optical fiber holder 10 and the in-situ droplet holder 5, and ensures that the light emitted by the xenon lamp 7 is fully irradiated on the in-situ droplet holder 5.

[0042] In this embodiment, there are two positioning posts 62, which are staggered, and the positioning holes 63 on the positioning plate 61 correspond to the positioning posts 62. The staggered positioning posts 62 play a multi-point positioning role for the upper base 1, and can achieve a better positioning effect.

[0043] Two oppositely arranged rotating support seats 13 are fixed to one side of the lower base 2 away from the original droplet seat 5. The two rotating support seats 13 jointly support a rotating shaft 14. The rotating shaft 14 passes through the upper base 1. A supporting boss 16 is fixed to the bottom surface of the upper base 1. The supporting boss 16 is arranged close to the rotating shaft 14. The rotating shaft 14 is arranged to facilitate the rotation of the upper base 1. At the same time, the supporting boss 16 is arranged to increase the supporting area of ​​the lower base 2 on the upper base 1, thereby ensuring the stability of the upper base 1 when it is flipped and closed.

[0044] A vertical rod 18 is provided in the upper shell 3, the bottom end of the vertical rod 18 is fixed to the upper surface of the upper base 1, and a support rod 19 is fixed to the top of the vertical rod 18, which is arranged obliquely, and the support rod 19 supports the optical fiber in the upper shell 3. The vertical rod 18 and the support rod 19 play a supporting and limiting role for the optical fiber, preventing the position of the optical fiber from changing during the frequent flipping of the upper base 1, which affects the use of the device. A plurality of through holes can be provided on the support rod 19 for binding the position of the optical fiber.

[0045] The LED seat 11 also includes a fluorescence lifting motor 12 and a turbidity lifting motor 17. The fluorescence LED module 111 and the turbidity LED module 112 are arranged in parallel. The output shafts of the fluorescence lifting motor 12 and the turbidity lifting motor 17 are respectively connected to the bottom of the fluorescence LED module 111 and the turbidity LED module 112. The arrangement of the fluorescence lifting motor 12 and the turbidity lifting motor 17 facilitates the switching of the fluorescence mode and the turbidity mode light sources. The fluorescence LED module 111 includes a blue LED light source, a collimator and a focusing lens, and the turbidity LED module 112 includes a yellow LED light source and a collimator. The light emitted by the blue LED light source passes through the collimator and the focusing lens to irradiate the sample of the in-situ droplet seat, and the light emitted by the yellow LED light source passes through the collimator to irradiate the sample of the in-situ droplet seat. The light source, the collimator and the focusing lens are not shown in the attached figure. The device also includes a control circuit, which includes a processor U1, a two-way LED constant current power supply circuit, a two-color LED circuit, a PD sampling circuit, and an optical switch module circuit. The processor U1 is electrically connected to the optical fiber spectrometer, the xenon lamp, the optical switch module, the PD sampling circuit, and the two-way LED constant current power supply circuit respectively. The two-way LED constant current power supply circuit is connected to the two-color LED circuit respectively. The two-color LED circuit includes the light source of the fluorescent LED module and the light source of the turbidity LED module. The PD sampling circuit includes an analog-to-digital conversion circuit and a power amplifier circuit. The photocell sensor includes a photocell PD1, and the photocell PD1 is connected to the processor U1 through the power amplifier circuit and the analog-to-digital conversion circuit. The processor U1 controls the operation of the entire device as the overall control center. The two-way LED constant current power supply circuit can provide a stable light source for the light source of the LED holder including the fluorescent LED module and the turbidity LED module. After receiving the corresponding optical signal, the optical fiber spectrometer and the PD sampling circuit send the data to the processor U1 after preliminary processing, and the processor U1 performs unified analysis and processing.

[0046] The two LED constant current power supply circuits are respectively a first constant current power supply circuit and a second constant current power supply circuit. The first constant current power supply circuit includes capacitor C8, capacitor C4, resistor R3, resistor R2, inductor L1, capacitor C1, resistor R1, capacitor C13, LED driver U3, capacitor C10, capacitor C9 and a 5V DC power supply.

[0047] Pin 19 of the processor U1 is connected to resistor R1, and the other end of resistor R1 is connected to pin 5 of the LED driver U3. A 5V DC power supply is connected to capacitor C9, capacitor C10 and pin 3 of the LED driver U3. The other ends of capacitor C9 and capacitor C10 are grounded. Pin 1 of the LED driver U3 is grounded. Pin 4 of the LED driver U3 is connected to capacitor C13 and resistor R2. The other end of capacitor C13 is grounded. The other end of resistor R2 is connected to resistor R3 and a common end. The other end of resistor R3 is grounded. Pin 6 of the LED driver U3 is connected to capacitor C1. The other end of capacitor C1 is connected to pin 2 of the LED driver U3 and inductor L1. The other end of inductor L1 is connected to the output end. Both ends of capacitor C8 and capacitor C4 are connected to the output common end respectively.

[0048] The connection structure of the second constant current power supply circuit is the same as that of the first constant current power supply circuit. The second constant current power supply circuit is connected to the pin 20 of the processor U1.

[0049] The first constant current power supply circuit and the second constant current power supply circuit are electrically connected to the dual-color LED circuit through the connector J2 and the connector J3. The connector J2 and the connector J3 are a pair of male and female connection ports.

[0050] The model of LED driver U3 is TPS54201, the value of capacitor C4 is 10μF, the value of capacitor C8 is 100nF, the value of inductor L1 is 68μH, the value of resistor R2 is 510R, and the value of resistor R1 is OR.

[0051] The optical switch module circuit includes resistor R7, resistor R8, resistor R6, switch driver U5, capacitor C26, and pin 17 of processor U1 is connected to resistor R7, the other end of resistor R7 is connected to resistor R6 and pin 1 of switch driver U5, the other end of resistor R6 is grounded, pin 2 of switch driver U5 is grounded, pin 18 of processor U1 is connected to resistor R8, the other end of resistor R7 is connected to pin 3 of switch driver U5, pin 5 of switch driver U5 is connected to +5V power supply and capacitor C26, the other end of capacitor C26 is grounded, pin 4 and pin 6 of switch driver U5 are respectively connected to M2 end and M1 end, and the M2 end and M1 end are optical switches of the optical fiber spectrometer, and the optical switch of the optical fiber spectrometer is used to control the switching of the filter.

[0052] The model of the switch driver U5 is AP1511A, the values ​​of the resistors R7 and R8 are 0R, the value of the resistor R6 is 10K, and the specification of the capacitor C26 is 1UF / 50V.

[0053] The analog-to-digital conversion circuit includes an analog-to-digital converter U6, a resistor R13, a resistor R12, a capacitor C29, a capacitor C30 and a resistor R21. Pin 26 and pin 27 of the processor U1 are respectively connected to pin 5 and pin 6 of the analog-to-digital converter U6. One end of the resistor R13 and the resistor R12 are respectively connected to pin 5 and pin 6 of the analog-to-digital converter U6. The other ends of the resistors R13 and R12 are grounded. Pin 7 of the analog-to-digital converter U6 is connected to a +5V power supply. One ends of the capacitors C29 and C30 are connected to pin 7 of the analog-to-digital converter U6. The other ends of the capacitors C29 and C30 are grounded. Pin 8 of the analog-to-digital converter U6 is connected to pin 1 of the analog-to-digital converter U6. Pin 2 of the analog-to-digital converter U6 is grounded. Pin 3 of the analog-to-digital converter U6 is connected to resistor R21. The other end of the resistor R21 is grounded.

[0054] The power amplifier circuit includes resistor R18, resistor R17, capacitor C35, operational amplifier U7B, resistor R16, operational amplifier U7A, resistor R20, resistor R14, capacitor C32, capacitor C33, resistor R19, capacitor C31, capacitor C28, resistor R11 and capacitor C22, pin 4 of operational amplifier U7A is grounded, pin 3 of operational amplifier U7A is connected to resistor R20, the other end of resistor R20 is grounded, pin 2 of operational amplifier U7A is connected to resistor R14 and capacitor C32, the other ends of resistor R14 and capacitor C32 are connected to the positive electrode of photocell PD1, one end of resistor R19 is connected to the positive electrode of photocell PD1, the other end of resistor R19 is grounded, the negative electrode of photocell PD1 is connected to +5V power supply, one end of capacitor C33 is connected to 5V power supply, the other end of capacitor C33 is grounded, pin 8 of operational amplifier U7A is connected to 5V power supply, capacitor C31 and capacitor C28, The other ends of capacitor C31 and capacitor C28 are grounded, pin 1 of operational amplifier U7A is connected to resistor R16, resistor R11 and resistor R9, the other end of resistor R9 is connected to capacitor C22, the other ends of resistor R11 and capacitor C22 are connected to pin 2 of operational amplifier U7A, the other end of resistor R16 is connected to pin 5 of operational amplifier U7B, pin 6 of operational amplifier U7B is grounded, pin 7 of operational amplifier U7B is connected to resistor R17, the other end of resistor R17 is grounded and connected to resistor R18, and the other end of resistor R18 is connected to pin 4 of analog-to-digital converter U6.

[0055] The models of operational amplifier U7B and operational amplifier U7A are OPA2350, and the model of analog-to-digital converter U6 is CS1237.

[0056] The PD sampling circuit further includes a capacitor C34 and a test point TP1 . The capacitor C34 and the test point TP1 are both connected to the resistor R18 and the pin 4 of the analog-to-digital converter U6 . The other end of the capacitor C34 is grounded.

[0057] The control circuit also includes a connector J4 and a connector J1. Pins 12 and 13 of the processor U1 are connected to the xenon lamp through the connector J4. Pins 30 and 31 of the processor U1 communicate with the spectrometer through the connector J1. The model of the processor U1 is STM32F030.

[0058] The control circuit also includes a voltage stabilizing circuit, which includes a voltage stabilizer U2, a capacitor C14, a capacitor C15, a capacitor C16, and a capacitor C17.

[0059] The voltage regulator circuit is used to provide a stable voltage for the processor U1. The model of the voltage regulator U2 is ME6118-3.3V.

[0060] When the present invention is used, the upper base 1 is turned outward, and the experimenter drops the sample solution on the in-situ droplet seat 5. The upper base 1 is turned inward, and the positioning column 62 enters the positioning hole 63. The positioning column 62 and the supporting boss 16 jointly support the upper base 1.

[0061] When performing ultra-trace detection, the processor U1 of the control circuit turns on the xenon lamp 7, and the light emitted by the xenon lamp 7 is transmitted to the upper light seat through the optical fiber, and then the optical fiber is vertically irradiated onto the sample of the in-situ droplet seat 5, and the generated light signal is transmitted to the optical fiber spectrometer 8 by the optical fiber, and the spectrum analyzer transmits the data to the processor U1 after preliminary processing;

[0062] When performing fluorescence detection, the processor U1 of the control circuit controls the light source of the fluorescent LED module of the dual-color LED circuit to turn on. After the ultraviolet light is focused by the collimating lens and the focusing lens, it is irradiated onto the sample of the in-situ droplet seat 5. After the sample absorbs the ultraviolet light, it emits a fluorescence signal. After the fluorescence signal is collected by the optical fiber, it enters the optical fiber spectrometer 8. A fluorescence filter is installed in the optical fiber spectrometer 8. After eliminating the ultraviolet excitation light, a pure fluorescence spectrum signal is obtained. After analysis by the optical fiber spectrometer 8, a fluorescence spectrum graph is obtained.

[0063] When performing turbidity detection, the processor U1 of the control circuit controls the light source of the turbidity LED module of the dual-color LED circuit to turn on. After being collimated by a collimator, it is emitted to the sample on the droplet holder. In the horizontal direction of 90°, a photocell PD1 is installed. The signal detected by the photocell PD1 is transmitted to the processor U1 through the power amplifier circuit and the analog-to-digital conversion circuit.

[0064] In the present invention, by arranging a xenon lamp 7, a fluorescent LED module 111 and a turbidity LED module 112 to illuminate the in-situ droplet seat 5, in-situ ultra-trace, fluorescence and turbidity measurements of the sample are performed, thereby achieving a one-machine-multiple-purpose function. The in-situ droplet seat 5 drips the sample, and the fiber optic spectrometer 8 receives the xenon lamp 7 and ultraviolet LED light irradiation through the optical fiber, and the sample generates a light signal. The photocell sensor 9 receives the infrared LED light irradiation light signal generated by the sample through the optical fiber, so that the measurement platform of the present invention can realize in-situ multifunctional detection of a drop of sample, which saves sample usage and avoids measurement errors and time differences caused by multiple sample transfers. The space is also fully utilized, which greatly simplifies the operator's difficulty, complexity and workload. The rotating connection on one side of the upper base 1 and the lower base 2 can flexibly open the upper base 1, which is convenient for the experimenter to operate.

[0065] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or terminal device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or terminal device. In the absence of further restrictions, the elements defined by the statement "include..." or "comprise..." do not exclude the existence of other elements in the process, method, article or terminal device including the elements. In addition, in this article, "greater than", "less than", "exceed" and the like are understood to exclude the number itself; "above", "below", "within" and the like are understood to include the number itself.

[0066] Although the above embodiments have been described, once those skilled in the art know the basic creative concepts, they can make additional changes and modifications to these embodiments. Therefore, the above description is only an embodiment of the present invention and does not limit the patent protection scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform, characterized in that: The invention comprises an upper base, a lower base, a xenon lamp, a fiber optic spectrometer and a photocell sensor. The upper base and the lower base are arranged opposite to each other. One side of the lower base is rotatably connected to one side of the upper base. An in-situ droplet seat is arranged on the other side of the lower base. An upper fiber optic seat corresponding to the position of the in-situ droplet seat is arranged on the upper base. An LED seat is arranged on the lower base. The LED seat comprises a fluorescent LED module and a turbidity LED module. The light emitted by the fluorescent LED module and the turbidity LED module irradiates the in-situ droplet seat. The xenon lamp is connected to the upper fiber optic seat of the upper base through an optical fiber. The fiber optic spectrometer and the photocell sensor are connected to the in-situ droplet seat through an optical fiber. It also includes a control circuit, which includes a processor U1, a two-way LED constant current power supply circuit, a two-color LED circuit, a PD sampling circuit and an optical switch module circuit. The processor U1 is electrically connected to the fiber optic spectrometer, the xenon lamp, the optical switch module, the PD sampling circuit and the two-way LED constant current power supply circuit respectively. The two-way LED constant current power supply circuit is connected to the two-color LED circuit respectively. The two-color LED circuit includes the fluorescent LED module and the turbidity LED module. The PD sampling circuit includes an analog-to-digital conversion circuit and a power amplifier circuit. The photocell sensor includes a photocell PD1. The photocell PD1 is connected to the processor U1 through a power amplifier circuit and an analog-to-digital conversion circuit.

2. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 1, characterized in that: The two LED constant current power supply circuits are respectively a first constant current power supply circuit and a second constant current power supply circuit. The first constant current power supply circuit includes capacitor C8, capacitor C4, resistor R3, resistor R2, inductor L1, capacitor C1, resistor R1, capacitor C13, LED driver U3, capacitor C10, capacitor C9 and a 5V DC power supply. Pin 19 of the processor U1 is connected to resistor R1, and the other end of resistor R1 is connected to pin 5 of the LED driver U3. A 5V DC power supply is connected to capacitor C9, capacitor C10 and pin 3 of the LED driver U3. The other ends of capacitor C9 and capacitor C10 are grounded. Pin 1 of the LED driver U3 is grounded. Pin 4 of the LED driver U3 is connected to capacitor C13 and resistor R2. The other end of capacitor C13 is grounded. The other end of resistor R2 is connected to resistor R3 and a common end. The other end of resistor R3 is grounded. Pin 6 of the LED driver U3 is connected to capacitor C1. The other end of capacitor C1 is connected to pin 2 of the LED driver U3 and inductor L1. The other end of inductor L1 is connected to the output end. Both ends of capacitor C8 and capacitor C4 are connected to the output common end respectively. The connection structure of the second constant current power supply circuit is the same as that of the first constant current power supply circuit. The second constant current power supply circuit is connected to the pin 20 of the processor U1. The first constant current power supply circuit and the second constant current power supply circuit are electrically connected to the dual-color LED circuit through the connector J2 and the connector J3, and the connector J2 and the connector J3 are a pair of male and female connection ports.

3. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform as claimed in claim 2, characterized in that: The model of the LED driver U3 is TPS54201, the value of the capacitor C4 is 10 μF, the value of the capacitor C8 is 100 nF, the value of the inductor L1 is 68 μH, the value of the resistor R2 is 510R, and the value of the resistor R1 is OR.

4. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 1, characterized in that: The optical switch module circuit includes resistor R7, resistor R8, resistor R6, switch driver U5, capacitor C26, and pin 17 of processor U1 is connected to resistor R7, the other end of resistor R7 is connected to resistor R6 and pin 1 of switch driver U5, the other end of resistor R6 is grounded, pin 2 of switch driver U5 is grounded, pin 18 of processor U1 is connected to resistor R8, the other end of resistor R7 is connected to pin 3 of switch driver U5, pin 5 of switch driver U5 is connected to +5V power supply and capacitor C26, the other end of capacitor C26 is grounded, pin 4 and pin 6 of switch driver U5 are respectively connected to M2 end and M1 end, and the M2 end and M1 end are optical switches of the optical fiber spectrometer, and the optical switch of the optical fiber spectrometer is used to control the switching of the filter.

5. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform as claimed in claim 4, characterized in that: The model of the switch driver U5 is AP1511A, the values ​​of the resistors R7 and R8 are 0R, the value of the resistor R6 is 10K, and the specification of the capacitor C26 is 1UF / 50V.

6. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 1, characterized in that: The analog-to-digital conversion circuit includes an analog-to-digital converter U6, a resistor R13, a resistor R12, a capacitor C29, a capacitor C30 and a resistor R21. Pin 26 and pin 27 of the processor U1 are respectively connected to pin 5 and pin 6 of the analog-to-digital converter U6. One end of the resistor R13 and the resistor R12 are respectively connected to pin 5 and pin 6 of the analog-to-digital converter U6. The other ends of the resistors R13 and R12 are grounded. Pin 7 of the analog-to-digital converter U6 is connected to a +5V power supply. One end of the capacitors C29 and C30 are connected to pin 7 of the analog-to-digital converter U6. The other ends of the capacitors C29 and C30 are grounded. Pin 8 of the analog-to-digital converter U6 is connected to pin 1 of the analog-to-digital converter U6. Pin 2 of the analog-to-digital converter U6 is grounded. Pin 3 of the analog-to-digital converter U6 is connected to resistor R21. The other end of the resistor R21 is grounded. The power amplifier circuit includes resistor R18, resistor R17, capacitor C35, operational amplifier U7B, resistor R16, operational amplifier U7A, resistor R20, resistor R14, capacitor C32, capacitor C33, resistor R19, capacitor C31, capacitor C28, resistor R11 and capacitor C22, pin 4 of operational amplifier U7A is grounded, pin 3 of operational amplifier U7A is connected to resistor R20, the other end of resistor R20 is grounded, pin 2 of operational amplifier U7A is connected to resistor R14 and capacitor C32, the other ends of resistor R14 and capacitor C32 are connected to the positive electrode of photocell PD1, one end of resistor R19 is connected to the positive electrode of photocell PD1, the other end of resistor R19 is grounded, the negative electrode of photocell PD1 is connected to +5V power supply, one end of capacitor C33 is connected to 5V power supply, the other end of capacitor C33 is grounded, pin 8 of operational amplifier U7A is connected to 5V power supply, capacitor C31 and capacitor C28, The other ends of capacitor C31 and capacitor C28 are grounded, pin 1 of operational amplifier U7A is connected to resistor R16, resistor R11 and resistor R9, the other end of resistor R9 is connected to capacitor C22, the other ends of resistor R11 and capacitor C22 are connected to pin 2 of operational amplifier U7A, the other end of resistor R16 is connected to pin 5 of operational amplifier U7B, pin 6 of operational amplifier U7B is grounded, pin 7 of operational amplifier U7B is connected to resistor R17, the other end of resistor R17 is grounded and connected to resistor R18, and the other end of resistor R18 is connected to pin 4 of analog-to-digital converter U6.

7. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 6, characterized in that: The models of operational amplifier U7B and operational amplifier U7A are OPA2350, and the model of analog-to-digital converter U6 is CS1237.

8. The in-situ ultra-micro, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 6, characterized in that: The PD sampling circuit also includes a capacitor C34 and a test point TP1. The capacitor C34 and the test point TP1 are both connected to the resistor R18 and the pin 4 of the analog-to-digital converter U6. The other end of the capacitor C34 is grounded.

9. The in-situ ultra-micro, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 1, characterized in that: The control circuit also includes a connector J4 and a connector J1. Pins 12 and 13 of the processor U1 are connected to the xenon lamp through the connector J4. Pins 30 and 31 of the processor U1 communicate with the spectrometer through the connector J1. The model of the processor U1 is STM32F030.

10. The in-situ ultra-trace, fluorescence, and turbidity multifunctional droplet measurement platform according to claim 1, characterized in that: The fluorescent LED module includes a blue LED light source, a collimator and a focusing lens, and the turbidity LED module includes a yellow LED light source and a collimator. The light emitted by the blue LED light source is irradiated onto the sample of the in-situ droplet seat through the collimator and the focusing lens, and the light emitted by the yellow LED light source is irradiated onto the sample of the in-situ droplet seat through the collimator.

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