Calibration sliding glass

Abstract

A chart for color calibration of a transmission type, characterized by having a transparent substrate and a color bar group formed on the transparent substrate, the color bar group being composed of a plurality of color bars of at least a first color and a second color arranged in a pattern in different orders, a coordinate point of the first color being located in a region surrounded by four points of (0.351, 0.649), (0.547, 0.453), (0.380, 0.506) and (0.433, 0.464) on an xy chromaticity diagram, a coordinate point of the second color being located in a region surrounded by four points of (0.125, 0.489), (0.112, 0.229), (0.270, 0.407) and (0.224, 0.242) on the xy chromaticity diagram, and the transmission spectrum of the first color color bar and the transmission spectrum of the second color color bar each having a separate peak.

Description

[0001] This application is a divisional application of application No. 202110650456.2 filed with the China National Intellectual Property Office on May 31, 2021 and entitled “Transmissive Color Calibration Chart and Calibration Sliding Glass”, which is a divisional application of application No. 201780020175.0 filed with the China National Intellectual Property Office on March 30, 2017 and entitled “Transmissive Color Calibration Chart and Calibration Sliding Glass”. TECHNICAL FIELD

[0002] The present application relates to a transmissive color calibration chart (hereinafter, sometimes abbreviated as a color chart) for a photographing apparatus, particularly a photographing apparatus for pathology, and a calibration sliding glass capable of suppressing the appearance of Newton's rings. BACKGROUND

[0003] In the field of photographing apparatuses, high resolution of output images has been advanced, and with respect to color, high color reproducibility that faithfully reproduces color tones is required.

[0004] A photographing apparatus displays an output image with correct reproduced colors, for example, by using a color chart disclosed in Patent Literature 1, comparing reproduced colors in the photographing apparatus and reproduced colors in the color chart, and performing calibration based on the color chart in the case where the reproduced colors are different.

[0005] A color chart used for color calibration includes color bars of arbitrary colors. For example, color bars of three primary colors of red, green, and blue can be included, and the kind of color bars can be appropriately selected in accordance with a color gamut (hereinafter, sometimes abbreviated as “gamut”) that a photographing apparatus can reproduce.

[0006] Here, a gamut refers to a specific range in a visible region, and for example, as shown in FIG. 1, can be expressed using an xy chromaticity diagram of XYZ colorimetric system (CIE 1931-XYZ colorimetric system) specified by CIE (Commission Internationale de l'Eclairage). A gamut can be expressed using chromaticity coordinates determined as vertices of R, G, and B colors in the xy chromaticity diagram and a triangle connecting the vertices by a straight line. Figure 11

[0007] A gamut is determined by various gamut standards in the related art, and for example, sRGB standard, NTSC standard, and the like can be cited. The sRGB standard is an international standard determined by IEC (International Electrotechnical Commission), and the NTSC standard is a gamut standard for analog television systems established by the National Television Standards Committee of the United States. Recently, new standards that encompass wider gamuts, such as Adobe RGB standard, D-Cinema Ref. PJ standard, have also emerged.

[0008] ​The photographing apparatus is designed to correspond to these color gamut standards, and can reproduce the pointer colors included in the color gamut of the photographing apparatus when outputting an image. The pointer colors are color measurement data indicating the color gamut of a real surface color. In a case where an image having accurate and consistent color reproduction is required, such as in a photographing apparatus for pathology, a wider color gamut is required so that a large number of the above-described pointer colors are included.

[0009] Prior Art Documents

[0010] Patent Documents

[0011] Patent Document 1: International Publication No. 2004 / 044639 SUMMARY

[0012] Problems to be Solved by the Invention

[0013] A color chart used in color calibration has a color bar group including a plurality of color bars. In order to correctly perform color calibration of a photographing apparatus, it is necessary to make the brightness of each color bar constituting the color bar group uniform, and in the related art, for example, a method of adjusting the brightness of each color bar acquired as an image by the photographing apparatus using a gray scale uniformly is known. However, the method of adjusting the brightness of each color bar using a gray scale has problems such as a complicated structure of the color chart.

[0014] In addition, photographing apparatuses generally have an IR cut filter in a camera. The IR cut filter differs in the wavelength range to be cut depending on the kind thereof. Therefore, a color chart used in color calibration of a photographing apparatus can differ in hue depending on the kind of the IR cut filter provided in the camera, and as a result, it is difficult to perform correct color calibration of the photographing apparatus.

[0015] The present invention has been achieved in view of the above problems, and a main object thereof is to provide a transmission type color calibration chart capable of performing correct color calibration of a photographing apparatus by making the brightness of a color bar group uniform and suppressing the influence of an IR cut filter provided in a camera of the photographing apparatus. Hereinafter, the transmission type color calibration chart will be sometimes referred to as a color chart.

[0016] Means for Solving the Problems

[0017] In order to solve the above problems, the present inventors and others have intensively studied and have obtained the following insight, that is, the transmission spectrum of a color bar constituting a color bar group, for example, as described below Figure 4(c) in the case where the transmission spectrum of the color bar is not a hill-shaped waveform, the luminance of the color bar becomes higher than the luminance of the other color bars. In addition, it is found that, in the case where the transmission spectrum of the color bar is not a hill-shaped waveform, the wavelength domain that is cut off depending on the kind of the IR cut filter changes, and thus the hue of the color bar is affected by the kind of the IR cut filter. Therefore, the inventors of the present application provide a transmission-type color calibration chart that, by setting the transmission spectrum of each color bar constituting a color bar group to be a hill-shaped waveform, can homogenize the brightness of the color bar group without using a gray scale, and can suppress the influence of the IR cut filter on the hue, and can perform correct color calibration of a photographing apparatus.

[0018] That is, the present application provides a transmission-type color calibration chart characterized by having a transparent substrate and a color bar group formed on the transparent substrate, the color bar group being constituted by a plurality of color bars of at least a first color and a second color arranged in a pattern in different orders, a coordinate point of the first color being located in a region surrounded by four points of (0.351, 0.649), (0.547, 0.453), (0.380, 0.506), and (0.433, 0.464) on an xy chromaticity diagram, a coordinate point of the second color being located in a region surrounded by four points of (0.125, 0.489), (0.112, 0.229), (0.270, 0.407), and (0.224, 0.242) on the xy chromaticity diagram, and the transmission spectrum of the first color color bar and the transmission spectrum of the second color color bar each having a separate peak top.

[0019] In addition, among the colors of the color bars, red, green, and blue are sometimes referred to as "primary colors". In addition, each color is sometimes marked with a short form in parentheses.

[0020] In addition, the chromaticity coordinate on the xy chromaticity diagram is sometimes simply referred to as a "color coordinate" or a "coordinate".

[0021] In addition, the first color is sometimes referred to as yellow (Ye), and the second color is sometimes referred to as cyan (Cy).

[0022] According to the present application, by the transmission spectrum of the first color color bar and the transmission spectrum of the second color color bar each having a separate peak top, it is possible to set the transmission spectrum of the first color color bar and the transmission spectrum of the second color color bar to be a hill-shaped waveform. Thereby, it is possible to homogenize the brightness of the color bar group, and suppress the influence of the IR cut filter possessed by the camera of the photographing apparatus, and thus it is possible to perform correct color calibration of the photographing apparatus.

[0023] In the above invention, the above color bar group further includes the above color bars of red, green, and blue, and preferably the transmission spectrum of the red color bar, the transmission spectrum of the green color bar, and the transmission spectrum of the blue color bar each have a separate peak top. The transmission spectrum of the color bar of the five colors other than white can be set to a hill-shaped waveform. Thereby, the brightness of the color bar group is further homogenized, and further the influence of the IR cut filter possessed by the camera of the photographing device is suppressed, and thus the photographing device can be color calibrated correctly.

[0024] In the above invention, preferably the inclusion rate of the pointer color included in the pentagon formed by connecting the chromaticity coordinates on the xy chromaticity diagram of the above color bars of at least five colors of red, green, blue, the above first color, and the above second color is 74.4% or more.

[0025] With regard to the color chart of the present invention, the color gamut defined by the coordinates of the above five colors sufficiently includes the pointer color, and thus the colors in the visible light region can be sufficiently captured. Thereby, the photographing device calibrated using the color chart of the present invention can reproduce the real object color with high accuracy and sufficiently.

[0026] In addition, the present invention provides a transmission type color calibration chart, characterized by having a transparent substrate and a color bar group formed on the above transparent substrate, the above color bar group is formed by arranging the color bars of at least six colors of red, green, blue, a first color, a second color, and white in a pattern in different orders, the coordinate point of the above first color is located in the region surrounded by (0.351, 0.649), (0.547, 0.453), (0.380, 0.506), and (0.433, 0.464) on the xy chromaticity diagram, the coordinate point of the above second color is located in the region surrounded by (0.125, 0.489), (0.112, 0.229), (0.270, 0.407), and (0.224, 0.242) on the xy chromaticity diagram, the peak wavelength of the transmission spectrum of the red color bar is in the range of 600 nm to 680 nm, the peak wavelength of the transmission spectrum of the green color bar is in the range of 495 nm to 570 nm, the peak wavelength of the transmission spectrum of the blue color bar is in the range of 430 nm to 490 nm, the peak wavelength of the transmission spectrum of the first color color bar is in the range of 540 nm to 595 nm, and the peak wavelength of the transmission spectrum of the second color color bar is in the range of 470 nm to 515 nm.

[0027] In other words, the present application provides a transmission type color calibration chart having a transparent substrate and a color bar group formed on the transparent substrate, the color bar group being composed of at least six color bars of red (R), green (G), blue (B), yellow (Ye), cyan (Cy), and white (W) arranged in a pattern in different orders, the peak wavelength of the transmission spectrum of the R color bar being in the range of 600 nm to 680 nm, the peak wavelength of the transmission spectrum of the G color bar being in the range of 495 nm to 570 nm, the peak wavelength of the transmission spectrum of the B color bar being in the range of 430 nm to 490 nm, the peak wavelength of the transmission spectrum of the Ye color bar being in the range of 540 nm to 595 nm, and the peak wavelength of the transmission spectrum of the Cy color bar being in the range of 470 nm to 515 nm.

[0028] According to the present application, the color bars of the five colors other than white have the peak wavelengths of the transmission spectrum at predetermined positions, so that the colors in the visible light region can be uniformly reproduced by the minimum required six colors in the xy chromaticity diagram for encompassing the desired pointer colors. Thus, the color chart of the present application can correctly perform color calibration of a photographing device.

[0029] In the above application, it is preferable that the color bar group further include color bars of two colors of violet (V) and near infrared (NIR), the relative transmittance of the transmission spectrum of the V color bar when the wavelength of 415 nm is set as a reference wavelength and the transmittance at the reference wavelength is 100% becomes 50% at a wavelength in the range of 435 nm to 465 nm, and the relative transmittance of the transmission spectrum of the NIR color bar when the wavelength of 730 nm is set as a reference wavelength and the transmittance at the reference wavelength is 100% becomes 50% at a wavelength in the range of 630 nm to 730 nm.

[0030] The color chart of the present application can sufficiently encompass the colors in the visible light region and uniformly reproduce the colors in the visible light region by further including color bars of two colors of violet and near infrared that represent predetermined transmission spectra. Thus, the accuracy of color calibration of a photographing device using the color chart of the present application can be improved.

[0031] Further, in the above application, it is preferable that the color bar group further include an orange (O) color bar, the peak wavelength of the transmission spectrum of the O color bar being in the range of 575 nm to 620 nm.

[0032] The color chart of the present application can compensate for the intermediate colors between yellow and red, which have a wide interval of peak wavelengths, and can sufficiently and uniformly encompass the colors in the visible light region by further including an orange color bar that represents a predetermined transmission spectrum, so that more refined and high-accuracy color calibration can be performed.

[0033] In the above invention, it is preferable that the inclusion rate of the pointer color included in a pentagon formed by connecting the chromaticity coordinates on the xy chromaticity diagram of at least five colors of R, G, B, the above first color, and the above second color be 90.3% or more.

[0034] The color chart of the present invention sufficiently includes the pointer color by the color gamut defined by the coordinates of the above five colors, and thus can sufficiently encompass the colors in the visible light region. Thus, the photographing device calibrated using the color chart of the present invention can reproduce the real object color with high precision and sufficiently.

[0035] In the above invention, it is preferable that the above color bar group further include a magenta (Mg) color bar. White is obtained by mixing red and cyan, or blue and yellow, which are in a symmetrical position with respect to the W coordinate on the xy chromaticity diagram (belonging to a complementary color relationship), and the color chart of the present invention can also obtain white by including the Mg color bar in the color bar group and mixing green and magenta belonging to a complementary color relationship at an appropriate ratio. Thus, the color chart of the present invention adjusts the same white using three references using a complementary color relationship, and thus can calibrate white more accurately.

[0036] According to the above invention, it is preferable that the chromaticity coordinate on the xy chromaticity diagram of the above Mg color bar be located on an extension line of a line connecting the chromaticity coordinate on the xy chromaticity diagram of the above G color bar and the white coordinate of D65 (hereinafter, sometimes referred to as the W(D65) coordinate). On the xy chromaticity diagram, by setting the position of the Mg coordinate to be on the extension line of the line connecting the W(D65) coordinate and the G coordinate, each combination of red and cyan, blue and yellow, and green and magenta belonging to a complementary color relationship is reproduced at an appropriate ratio as white of D65. Thus, the color chart of the present invention can calibrate white and adjust white balance more accurately.

[0037] In the above invention, it is preferable that the peak wavelength of the transmission spectrum of the above R color bar be in the range of 620 nm to 680 nm, and the half-width be 100 nm or less, the peak wavelength of the transmission spectrum of the above G color bar be in the range of 510 nm to 540 nm, and the half-width be 80 nm or less, and the peak wavelength of the transmission spectrum of the above B color bar be in the range of 450 nm to 472 nm, and the half-width be 80 nm or less.

[0038] The transmission spectrum of the color bars of the three primary colors has the spectral characteristics as described above, so that on the xy chromaticity diagram, the color gamut defined by the triangle formed by connecting the chromaticity coordinates of the three primary colors can be made close to the color gamut defined by the coordinates of the three primary colors in the BT.2020 standard. Thus, the color chart of the present application can cope with color calibration of a shooting device that applies the BT.2020 standard. Furthermore, the waveform of the transmission spectrum is given various waveform shapes for each color coordinate, so that by setting the half-width in matching with the position of the peak wavelength of the transmission spectrum for each color, the color chart of the present application can ensure the color reproducibility of the transmission spectrum.

[0039] In the above-described application, it is preferable that the chromaticity coordinate of the above-described second color color bar on the xy chromaticity diagram be on an extension line of a line connecting the chromaticity coordinate of the above-described R color bar on the xy chromaticity diagram and the W (D65) coordinate, and be on the outer periphery of the color gamut defined under the BT.2020 standard or outside the above-described color gamut, and the chromaticity coordinate of the above-described first color color bar on the xy chromaticity diagram be on an extension line of a line connecting the chromaticity coordinate of the above-described B color bar on the xy chromaticity diagram and the above-described W (D65) coordinate, and be on the outer periphery of the color gamut defined under the BT.2020 standard or outside the above-described color gamut.

[0040] This is because, on the xy chromaticity diagram, by making the position of the second color coordinate be on an extension line of a line connecting the W (D65) coordinate and the R coordinate, and making the position of the first color coordinate be on an extension line of a line connecting the W (D65) coordinate and the B coordinate, it is possible to reproduce the white color of D65 by mixing each combination of cyan and red or yellow and blue belonging to the complementary color relationship at an appropriate ratio, and it is possible to adjust the white balance. Furthermore, by making the positions of the second color coordinate and the first color coordinate be on the outer periphery of the color gamut defined under the BT.2020 standard or outside the above-described color gamut, it is possible to expand the color gamut in which color reproduction can be performed using the color chart of the present application.

[0041] Further, the present application provides a calibration slide glass characterized by comprising: a calibration pattern having a plurality of calibration pattern chips; a first spacer disposed around the calibration pattern; a pair of protective substrates disposed so as to face each other with the calibration pattern and the first spacer interposed therebetween, the area overlapping the calibration pattern in plan view having at least a transmission portion; and a second spacer disposed so as to overlap a part of the calibration pattern chips in plan view between the protective substrate of at least one of the first spacer and the pair of protective substrates and the first spacer, the second spacer having at least an opening portion in the area overlapping the calibration pattern in plan view.

[0042] According to the above invention, by the second spacer having at least an opening portion in an area overlapping the calibration pattern in plan view, a gap equivalent to the thickness of the second spacer can be provided between the calibration pattern and the transparent portion of the protective substrate on which the second spacer is arranged, and a calibration sliding glass that can suppress the occurrence of Newton's rings can be produced.

[0043] In the above invention, it is preferable that a sealing portion be arranged along the outer periphery of the protective substrate between the pair of protective substrates. This is because the side surfaces of the first and second spacers can be prevented from being exposed, and thus the mechanical strength can be improved.

[0044] In the above invention, it is preferable that a groove be provided on the surface of the second spacer. This is because the increase in thickness caused by the arrangement of an adhesive on the surface of the second spacer can be suppressed, and the groove can be used as a release groove in the case of using a liquid adhesive.

[0045] In the above invention, it is preferable that an origin mark be provided on the surface of the protective substrate of one of the pair of protective substrates. Since the position information of the calibration pattern and the like can be identified based on the origin mark, the calibration sliding glass can be one that can cope with an automatic switching function.

[0046] In the above invention, it is preferable that the transparent portion of the protective substrate of one of the pair of protective substrates be arranged within the area of the transparent portion of the other protective substrate in plan view. This is because the outline of the calibration pattern chip when the calibration sliding glass is viewed from a prescribed surface can be clearly defined, and the calibration sliding glass can be one of high quality.

[0047] In the above invention, it is preferable that the optical path length in the thickness direction of all of the calibration pattern chips that are the same measurement target among the plurality of calibration pattern chips be uniform. This is because, by making the optical path length in the thickness direction of all of the calibration pattern chips that are the same measurement target uniform, higher-precision measurement can be performed by an observation device using a camera device equipped with the calibration sliding glass having the calibration pattern chips.

[0048] Effects of the Invention

[0049] The transmission-type color calibration chart of the present invention can perform correct color calibration of a photographing device by making the brightness of the color bar group uniform and suppressing the influence of an IR cut filter provided in a camera of the photographing device on the color tone. BRIEF DESCRIPTION OF DRAWINGS

[0050] Figure 1 is a schematic plan view showing an example of the transmission-type color calibration chart of the present invention.

[0051] Figure 2 is an image chart showing an example of a spectral transmission spectrum shown in the transmission type color calibration chart of the present application.

[0052] Figure 3 is an xy chromaticity chart showing an example of a color gamut of the transmission type color calibration chart of the present application.

[0053] Figure 4 is an explanatory view explaining a method of defining a peak wavelength and a reference wavelength of a transmission spectrum.

[0054] Figure 5 is a schematic plan view showing an example of the transmission type color calibration chart of the present application.

[0055] Figure 6 is a chart showing another example of a spectral transmission spectrum shown in the transmission type color calibration chart of the present application.

[0056] Figure 7 is an xy chromaticity chart showing another example of a color gamut of the transmission type color calibration chart of the present application.

[0057] Figure 8 is a schematic plan view showing an example of the transmission type color calibration chart of the present application.

[0058] Figure 9 is a chart showing another example of a spectral transmission spectrum shown in the transmission type color calibration chart of the present application.

[0059] Figure 10 is an xy chromaticity chart explaining positions of respective chromaticity coordinates of Cy, Ye and Mg in a color gamut of the transmission type color calibration chart of the present application.

[0060] Figure 11 is an xy chromaticity chart explaining a color gamut.

[0061] Figure 12 is a schematic plan view showing an example of the calibration sliding glass of the present application.

[0062] Figure 13 is an explanatory view for explaining the calibration sliding glass of the present application.

[0063] Figure 14 is a schematic sectional view showing another example of the calibration sliding glass of the present application.

[0064] Figure 15 is a schematic sectional view showing another example of the calibration sliding glass of the present application.

[0065] Figure 16 is an explanatory view for explaining a groove in the present application.

[0066] Figure 17 is a schematic cross-sectional view showing another example of the calibration sliding glass of the present application.

[0067] Figure 18 is an explanatory view for explaining the seal portion in the present application.

[0068] Figure 19 is an explanatory view for explaining the origin mark in the present application.

[0069] Figure 20 is an explanatory view for explaining the calibration mark in the present application.

[0070] Figure 21 is a schematic cross-sectional view showing an example of the calibration sliding glass in the related art.

[0071] Figure 22 is an explanatory view for explaining the calibration sliding glass of the present application.

[0072] Figure 23 is an explanatory view for explaining the calibration sliding glass of the present application.

[0073] Figure 24 is an explanatory view for explaining the calibration sliding glass of the present application.

[0074] Figure 25 is an explanatory view for explaining the calibration sliding glass of the present application.

[0075] Figure 26 is an explanatory view for explaining the calibration sliding glass of the present application.

[0076] Figure 27 is a graph showing the measurement results of the calibration sliding glass using Figure 15 DETAILED DESCRIPTION

[0077] The transmission type color calibration chart and the calibration sliding glass of the present application are explained.

[0078] I. Transmission type color calibration chart (color chart)

[0079] The color chart of the present application is explained in the first embodiment and the second embodiment.

[0080] I-1. First embodiment

[0081] ​The color chart of the first embodiment will be described in detail. The color chart of the present embodiment is characterized in that it has a transparent substrate and a color bar group formed on the transparent substrate, the color bar group is constituted of a plurality of color bars including at least a first color and a second color arranged in a pattern in different orders, a coordinate point of the first color is located in a region surrounded by four points of (0.351, 0.649), (0.547, 0.453), (0.380, 0.506), and (0.433, 0.464) on an xy chromaticity diagram, a coordinate point of the second color is located in a region surrounded by four points of (0.125, 0.489), (0.112, 0.229), (0.270, 0.407), and (0.224, 0.242) on the xy chromaticity diagram, and the transmission spectrum of the first color bar and the transmission spectrum of the second color bar each have a separate peak.

[0082] The color chart of the present embodiment will be described with reference to the drawings. Figure 1 is a schematic plan view showing an example of the color chart of the present embodiment. In addition, Figure 2 is a graph showing an example of the spectral transmission of the color chart of the present embodiment. In addition, regarding Figure 1 and Figure 2 Since the same content as that described in the item of "II. Second Embodiment" to be described later can be applied, the description thereof is omitted here.

[0083] In the related art, for example, as a method of adjusting the brightness of each color bar uniformly, a method using a gray scale is known. However, in the case of adjusting the brightness of each color bar using a gray scale, for example, there is a problem that the structure of the color chart becomes complicated. In addition, a photographing device generally has an IR cut filter in a camera. The wavelength range to be cut differs depending on the kind of the IR cut filter. Therefore, the color chart used in color calibration of a photographing device can differ in hue depending on the kind of the IR cut filter provided in the camera, and as a result, there is a problem that it is difficult to perform correct color calibration of the photographing device.

[0084] Therefore, the inventors of the present embodiment have obtained the insight that the transmission spectrum of the color bar constituting the color bar group, for example, as described in the item of "III. Third Embodiment" to be described later, is different from that of the color bar constituting the color bar group of the related art. Figure 4(c) In cases where the waveform is not mountain-shaped, the brightness of that color bar will be higher than that of the other color bars. Furthermore, it has been observed that when the transmission spectrum of a color bar is not mountain-shaped, the wavelength range cut off varies depending on the type of IR cutoff filter, thus affecting the hue of the color bar. This method is based on this observation, characterized in that the transmission spectra of the first color bar and the second color bar each have separate peaks. Therefore, in this method, the brightness of the color bar group can be homogenized, and the influence of the IR cutoff filter of the camera in the shooting device on the hue can be suppressed. Thus, in this method, accurate color calibration of the shooting device can be performed.

[0085] Furthermore, according to this method, a first color and a second color of predetermined color are added to the existing color bar group, thereby encompassing colors within the visible light region and consistently reproducing colors within that region. Therefore, the color chart using this method can be used for accurate color calibration of the imaging equipment.

[0086] In the following description, "having separated peaks" means that the appearance of the transmission spectrum is mountain-shaped, a so-called mountain-shaped waveform, for example, as described later. Figure 4 (a) shows the shape. Here, “the appearance shape is mountain-shaped” means that at least when the transmission spectrum of the visible area is viewed as a whole, it is mountain-shaped. For example, when the transmittance (%) fluctuates within a range of several percent, the waveform will not be mountain-shaped.

[0087] Furthermore, in the case of "having separate peaks", in the transmission spectrum, for example, as described later. Figure 2 As shown in the transmission spectrum of each color bar, it can have a mountain-shaped protrusion, or, as described later. Figure 9 As shown in the transmission spectrum of the magenta stripe, it may have two or more mountain-shaped protrusions, but in this embodiment, it is preferable to have at least one mountain-shaped protrusion in the visible area.

[0088] Therefore, "the transmission spectrum of each color bar has a separate peak" means, for example, as described later. Figure 2 As shown, each color of the transmission spectrum has an independent mountain-shaped waveform.

[0089] The following explains the structure of the color charts in this method.

[0090] A. Color stripes

[0091] In this method, the color stripes are formed on a transparent substrate.

[0092] The color bar group described above is composed of color bars of a plurality of colors including at least a first color and a second color arranged in a pattern in different orders. The color bar group of the present mode is preferably composed of color bars of at least six colors of R, G, B, Ye, Cy, and W arranged in a pattern in different orders.

[0093] In addition, "5 colors other than W" means R, G, B, Ye, and Cy. In the present specification, "5-color color bar" means the 5-color color bar described above unless otherwise specified. Furthermore, "6-color color bar" means a total of 6 colors of the 5-color color bar and W unless otherwise specified. The same applies to the case where other color color bars are included as described later.

[0094] 1. Transmission spectrum

[0095] The transmission spectrum of the first color color bar and the transmission spectrum of the second color color bar each have a separate peak top and can be a hill-shaped waveform. Furthermore, generally, the transmission spectrum of the R, G, and B color bars each also have a separate peak top and can be a hill-shaped waveform.

[0096] (1) Method of specifying peak wavelength

[0097] The transmission spectrum of the 5-color color bar other than W has a peak wavelength or a reference wavelength depending on the waveform. In addition, regarding the specific method of specifying the peak wavelength, the same as the content described in "II. Second Embodiment A. Color bar group 1. Transmission spectrum (1) Method of specifying peak wavelength" described later is applied, and the description thereof is omitted here.

[0098] (2) Spectral characteristics of each color bar

[0099] Hereinafter, the spectral characteristics of each color bar constituting the color bar group will be described.

[0100] (a) Red (R) color bar

[0101] The R color bar has selective transmission properties with respect to red light in a light source.

[0102] It is preferable that the transmission spectrum of the R color bar have a separate peak top. That is, it is preferable that the transmission spectrum of the R color bar be a hill-shaped waveform. By further uniformizing the brightness of the color bar group and further suppressing the influence of the IR cut filter provided in the camera of the imaging device, it is possible to perform correct color calibration of the imaging device.

[0103] The peak wavelength of the transmission spectrum of the R color stripe is preferably in the range of, for example, 600 nm to 680 nm, more preferably in the range of 610 nm to 680 nm, and particularly preferably in the range of 625 nm to 680 nm. This is because, in the case where the above peak wavelength is not in the above range, when the transmission spectra of the five colors excluding W are uniformly arranged, the separation of the peak tops is not clear, and it is difficult to achieve an interpolation color effect.

[0104] Since the other detailed description of the R color stripe is the same as that described in the item "II. Second Embodiment A. Color Stripe Group 1. Spectral characteristics (a) Red (R) color stripe" described later, the description thereof is omitted here.

[0105] (b) Green (G) color stripe

[0106] The G color stripe has selective transmissivity to green light in a light source.

[0107] The transmission spectrum of the G color stripe preferably has a separated peak top. That is, the transmission spectrum of the G color stripe is preferably a hill-shaped waveform. This is because, by further uniformizing the brightness of the color stripe group and further suppressing the influence of an IR cut filter provided in a camera of a photographing device, it is possible to perform correct color calibration of the photographing device.

[0108] The peak wavelength of the transmission spectrum of the G color stripe is preferably in the range of, for example, 495 nm to 570 nm, more preferably in the range of 505 nm to 550 nm, and particularly preferably in the range of 510 nm to 540 nm. This is because, in the case where the above peak wavelength is not in the above range, when the transmission spectra of the five colors excluding W are uniformly arranged, the separation of the peak tops is not clear, and it is difficult to achieve an interpolation color effect.

[0109] Since the other detailed description of the G color stripe is the same as that described in the item "II. Second Embodiment A. Color Stripe Group 1. Spectral characteristics (b) Green (G) color stripe" described later, the description thereof is omitted here.

[0110] (c) Blue (B) color stripe

[0111] The B color stripe has selective transmissivity to blue light in a light source.

[0112] The transmission spectrum of the B color stripe preferably has a separated peak top. That is, the transmission spectrum of the B color stripe is preferably a hill-shaped waveform. This is because, by further uniformizing the brightness of the color stripe group and further suppressing the influence of an IR cut filter provided in a camera of a photographing device, it is possible to perform correct color calibration of the photographing device.

[0113] The peak wavelength of the transmission spectrum of the B color stripe is preferably in the range of 430 nm to 490 nm, more preferably in the range of 435 nm to 475 nm, and particularly preferably in the range of 450 nm to 470 nm. This is because, in the case where the peak wavelength is not in the above range, when the transmission spectra of the 5 colors other than W are uniformly arranged, the separation of the peak tops is not clear, and it is difficult to achieve an interpolation color effect.

[0114] Since the other detailed description of the B color stripe can be the same as that described in the item "II. Second Embodiment A. Color Stripe Group 1. Spectral Characteristics (c) Blue (B) Color Stripe" described later, the description thereof is omitted here.

[0115] (d) First Color Stripe

[0116] The coordinate point of the first color stripe of the present embodiment on the xy chromaticity diagram is located in the region surrounded by the four points of (0.351, 0.649), (0.547, 0.453), (0.380, 0.506), and (0.433, 0.464), more preferably in the region surrounded by the four points of (0.417, 0.583), (0.490, 0.510), (0.387, 0.501), and (0.421, 0.474), and particularly preferably in the region surrounded by the four points of (0.435, 0.565), (0.472, 0.528), (0.402, 0.504), and (0.421, 0.489).

[0117] Further, the transmission spectrum of the first color stripe has a separated peak top. That is, the transmission spectrum of the first color stripe is a hill-shaped waveform. Thereby, by further uniformizing the brightness of the color stripe group and further suppressing the influence of the IR cut filter provided in the camera of the imaging device, it is possible to perform correct color calibration of the imaging device.

[0118] Hereinafter, the first color stripe will be sometimes referred to as a Ye color stripe.

[0119] Since the other detailed description of the Ye color stripe can be the same as that described in the item "II. Second Embodiment A. Color Stripe Group 1. Spectral Characteristics (d) Yellow (Ye) Color Stripe" described later, the description thereof is omitted here.

[0120] (e) Second Color Stripe

[0121] The coordinate point of the second color color bar of the present mode on the xy chromaticity diagram is located in a region surrounded by four points of (0.125, 0.489), (0.112, 0.229), (0.270, 0.407), and (0.224, 0.242), is particularly preferably located in a region surrounded by four points of (0.123, 0.437), (0.115, 0.296), (0.254, 0.350), and (0.240, 0.297), and is particularly preferably located in a region surrounded by four points of (0.133, 0.384), (0.130, 0.320), (0.239, 0.341), and (0.231, 0.312).

[0122] Further, the transmission spectrum of the second color color bar has a separate peak top. That is, the transmission spectrum of the second color color bar is a hill-shaped waveform. Thereby, by further homogenizing the brightness of the color bar group and further suppressing the influence of an IR cut filter possessed by a camera of a photographing device, correct color calibration of the photographing device can be performed.

[0123] Hereinafter, the second color color bar will be sometimes referred to as a Cy color bar and described.

[0124] Since other detailed description of the Cy color bar can be the same as the content described in the item "II. Second Embodiment A. Color Bar Group 1. Spectral Characteristics (2) of Each Color Bar (e) Cyan (Cy) Color Bar" described later, the description here is omitted.

[0125] (f) White (W) Color Bar

[0126] The W color bar is colorless and has light transmittance and is used as a blank. Further, since the W color bar can be the same as the content described in the item "II. Second Embodiment A. Color Bar Group 1. Spectral Characteristics (2) of Each Color Bar (f) White (W) Color Bar" described later, the description here is omitted.

[0127] (g) Arbitrary Color Bar

[0128] It is preferable that the above color bar group include two color bars of purple (V) and near infrared (NIR) having predetermined transmission spectra in addition to the above six color bars. The V color bar and the NIR color bar representing predetermined transmission spectra are further included on the basis of the five color bars other than the W, and thus the color chart of the present mode can sufficiently encompass colors in the visible light region and can consistently reproduce colors in the visible light region. Thereby, the accuracy of color calibration of a photographing device using the color chart of the present mode can be improved.

[0129] Further, it is preferable that the above color bar group include an orange (O) color bar having a predetermined transmission spectrum in addition to the above 6 color bars. The color chart of this mode further includes the O color bar indicating the predetermined transmission spectrum, so that the intermediate color of yellow and red having a wide interval of peak wavelengths can be compensated for, and the colors in the visible light region can be sufficiently and uniformly covered, so that color calibration can be performed in more detail and with higher accuracy.

[0130] Hereinafter, since the structure when including an arbitrary color bar is the same as the description of the use of the "transmission spectrum (2) of each color bar" described in the item of "II. Second Embodiment A. Color bar group 1. Spectral characteristics (g) of transmission spectrum (2) of each color bar" described later, the description thereof is omitted here. Figure 5-9

[0131] Hereinafter, each color bar that the color chart of this mode can include will be described.

[0132] (i) Violet (V) color bar

[0133] Preferably, the transmission spectrum of the V color bar has selective transmissivity for light on the side of shorter wavelengths than blue light in the light source, so as to be located on the side of shorter wavelengths than the transmission spectrum of the B color bar, and a part of which overlaps the transmission spectrum of the B color bar.

[0134] Further, it is preferable that the transmission spectrum of the V color bar have a separate peak top. That is, it is preferable that the transmission spectrum of the V color bar be a hill-shaped waveform. This is because, by further uniformizing the brightness of the color bar group and further suppressing the influence of the IR cut filter provided in the camera of the imaging device, correct color calibration of the imaging device can be performed.

[0135] Since other detailed description of the V color bar can be the same as the content described in the item of "II. Second Embodiment A. Color bar group 1. Spectral characteristics (i) of violet (V) color bar" described later, the description thereof is omitted here.

[0136] (ii) Near-infrared (NIR) color bar

[0137] Preferably, the transmission spectrum of the NIR color bar has selective transmissivity for light on the side of longer wavelengths than red light in the light source, so as to be located on the side of shorter wavelengths than the transmission spectrum of the R color bar, and a part of which overlaps the transmission spectrum of the B color bar.

[0138] Since other detailed description of the NIR color bar can be the same as the content described in the item of "II. Second Embodiment A. Color bar group 1. Spectral characteristics (ii) of near-infrared (NIR) color bar" described later, the description thereof is omitted here.

[0139] (iii) Orange (O) color bar ​

[0140] Preferably, the transmission spectrum of the O color bar has selective transmittance of light from the side of the long-wavelength light in the light source that is shorter than the red light, thus falling between the transmission spectrum of the Ye color bar and the transmission spectrum of the R color bar.

[0141] Because the peak wavelengths of the transmission spectrum of the Ye color bar and the R color bar are widely spaced, the peak wavelength of the transmission spectrum of the O color bar is positioned at the aforementioned location, thereby compensating for the intermediate colors of Ye and R and reproducing the aforementioned intermediate colors.

[0142] Furthermore, the preferred O color bar has a transmission spectrum with distinct peaks. That is, the preferred O color bar has a mountain-shaped transmission spectrum. This is because, by further homogenizing the brightness of the color bar group and further suppressing the influence of the IR cutoff filter of the camera in the shooting device, correct color calibration of the shooting device can be achieved.

[0143] Since the other detailed descriptions of the O color bar are the same as those described in the "II. Second Embodiment A. Color Bar Group 1. Transmission Spectrum (2) Spectral Characteristics of Each Color Bar (iii) Orange (O) Color Bar" section described later, the descriptions here are omitted.

[0144] (iv) Magenta (Mg) stripe

[0145] Preferably, the above-mentioned color bar group includes magenta (Mg) color bars.

[0146] On the xy chromaticity diagram of the color chart based on this method, the position of the W coordinate can be determined according to the positional relationship between the R and Cy coordinates and the positional relationship between the B and Ye coordinates. Furthermore, considering the positional relationship between the G and Mg coordinates, the position of the W coordinate of D65, which serves as the reference color, can be determined more accurately. In particular, it has the advantage that even when using a light source other than D65, the position of the W(65) coordinate can be correctly adjusted by using the color chart of this method, which includes the Mg color bar.

[0147] Mg color bars exhibit selective transmittance of red and blue light from a light source.

[0148] The preferred transmission spectrum of Mg color bar 22 (Mg) is as follows Figure 9 As shown, there is a first transmission peak (hereinafter referred to as the first peak wavelength) near the peak wavelength of the transmission spectrum 22 (R) of the R color bar, and a second transmission peak (hereinafter referred to as the second peak wavelength) near the peak wavelength of the transmission spectrum 22 (B) of the B color bar.

[0149] The transmission spectrum of the Mg color bar preferably has separate peak tops. That is, the 1st transmission peak and the 2nd transmission peak of the O color bar are preferably hill-shaped. This is because, by further homogenizing the brightness of the color bar group and further suppressing the influence of the IR cut filter possessed by the camera of the photographing device, correct color calibration of the photographing device can be performed.

[0150] Further detailed description of the Mg color bar can be the same as that described in the item "II. 2nd Embodiment A. Color bar group 1. Spectral characteristics (2) of each color bar of the transmission spectrum (iv) Magenta (Mg) color bar" described later, and thus the description here is omitted.

[0151] (h) Others

[0152] In the spectroscopic transmission spectrum of the color chart of the present embodiment, by repeating a part of the adjacent transmission spectrum in the visible light region, the mixing ratio of each color component contained in the mixed color can be correctly determined, and the color reproduction accuracy and the color calibration accuracy of the mixed color can be improved.

[0153] 2. xy Chromaticity Diagram

[0154] Next, the xy chromaticity diagram of the color chart of the present embodiment shown by the above color bar group is described. In addition, the xy chromaticity diagram in the present embodiment can be the same as that described in the item "II. 2nd Embodiment A. Color bar group 2. xy Chromaticity diagram" described later, and thus the description here is omitted.

[0155] 3. Structure of Color Bar and Color Bar Group

[0156] Each color bar can be a component that can express the desired transmission spectrum, and for example, a band pass filter, a dyed substrate can be used. In addition, the structure of the color bar and the color bar group in the present embodiment can be the same as that described in the item "II. 2nd Embodiment A. Color bar group 3. Structure of color bar and color bar group" described later, and thus the description here is omitted.

[0157] B. Light Shielding Portion

[0158] The color chart of the present embodiment is generally provided with a light shielding portion on the outer periphery of the above color bar group on the above transparent substrate. In addition, the light shielding portion in the present embodiment can be the same as that described in the item "II. 2nd Embodiment B. Light shielding portion" described later, and thus the description here is omitted.

[0159] C. Transparent Substrate

[0160] As the transparent substrate in the present embodiment, there is no particular limitation as long as it can support the color bar group and the light shielding portion and has a desired light transmittance, and it can be the same as the transparent substrate used for a color chart known in the art. Also, the transparent substrate in the present embodiment can be the same as the content described in "II. 2nd Embodiment C. Transparent Substrate" described later, and thus the description thereof is omitted.

[0161] D. Others

[0162] The color chart of the present embodiment can have a calibration mark, an identification code, a cover glass, a color bar holding frame, a transparent protective sheet with a light shielding portion, and the like in addition to the above-described structure. Also, the other structure in the present embodiment can be the same as the content described in "II. 2nd Embodiment D. Others" described later, and thus the description thereof is omitted.

[0163] E. Use

[0164] The color chart of the present embodiment can be used in a photographing device, a video device, and a peripheral device required for color calibration in general. It is particularly suitable for use in a photographing device for pathology.

[0165] I-2. 2nd Embodiment

[0166] The color chart of the 2nd embodiment is described in detail. The color chart of the present embodiment is characterized by having a transparent substrate and a color bar group formed on the transparent substrate, the color bar group being composed of color bars of at least six colors of red, green, blue, a first color, a second color, and white arranged in a pattern in different orders, a coordinate point of the first color being located in a region surrounded by four points of (0.351, 0.649), (0.547, 0.453), (0.380, 0.506), and (0.433, 0.464) on an xy chromaticity diagram, a coordinate point of the second color being located in a region surrounded by four points of (0.125, 0.489), (0.112, 0.229), (0.270, 0.407), and (0.224, 0.242) on the xy chromaticity diagram, a peak wavelength of a transmission spectrum of the red color bar being in a range of 600 nm to 680 nm, a peak wavelength of a transmission spectrum of the green color bar being in a range of 495 nm to 570 nm, a peak wavelength of a transmission spectrum of the blue color bar being in a range of 430 nm to 490 nm, a peak wavelength of a transmission spectrum of the first color color bar being in a range of 540 nm to 595 nm, and a peak wavelength of a transmission spectrum of the second color color bar being in a range of 470 nm to 515 nm.

[0167] In the related art, in the field of photographing devices, high resolution of output images has been advanced, and high color reproducibility that faithfully reproduces color tones is required with respect to colors.

[0168] In particular, a photographing apparatus for pathology is required to reproduce the color of an output image correctly close to the color of an actual object because it performs observation of a human tissue and pathological diagnosis based on an image captured by a microscope. In addition, in a case where the same sample is captured using different photographing apparatuses, if a difference occurs in the reproduced color due to a difference in the spectral sensitivity characteristic of the photographing apparatus, the same result cannot be obtained in the pathological diagnosis of the same sample based on the output image. Therefore, the reproduced color of the output image must be the same regardless of the type or manufacturer of the photographing apparatus.

[0169] In order to improve the color reproducibility of an output image in a photographing apparatus, it is necessary to widen the color gamut that can be reproduced by the photographing apparatus.

[0170] In order for a photographing apparatus to display an output image with accurate reproduced color, for example, a color chart disclosed in Patent Literature 1 is used to compare the reproduced color in the photographing apparatus and the reproduced color in the color chart, and in a case where there is a difference in the reproduced color, calibration is performed based on the color chart. As described above, a photographing apparatus for pathology is required to have an image output with correct and consistent color reproducibility, and therefore the color chart used in the color calibration of an output image is also required to have a wider color gamut.

[0171] However, the wider the color gamut of a photographing apparatus, the greater the number of reproducible colors, and therefore it is necessary to reproduce the pointer color more correctly. In the color chart in the related art, all colors in the visible light region are not sufficiently covered, and there are colors that cannot be reproduced when performing color calibration of an output image based on a photographing apparatus, particularly a photographing apparatus for pathology. Therefore, color calibration of a photographing apparatus cannot be performed correctly, and there is a problem in that a photographing apparatus cannot achieve high color reproducibility of an output image.

[0172] On the other hand, in order to reproduce a wide color gamut in a display or the like, a technique of developing a so-called "unimodal" spectral shape by reducing the overlap of the spectral of RGB three primary colors by a color filter or a backlight, thereby reducing the color mixture between adjacent colors has been developed.

[0173] In a color chart, in order to achieve a wide color gamut, the three primary colors need to be developed to have a spectral shape with a narrow distribution width of a transmission spectrum and a unimodal shape in principle as in a display or the like.

[0174] In addition to this, in the development of a color chart, the arrangement and transmission spectrum of colors with respect to the wavelength region between B-G and G-R in addition to the three primary colors must be studied so that the function of the chart can be improved and color calibration of an image sensor of a photographing apparatus can be performed.

[0175] In particular, a photographing apparatus for pathology requires high color reproducibility for an output image as described above, and requires consistent color reproducibility regardless of the kind of photographing apparatus, and thus a color chart used for the photographing apparatus for pathology needs to sufficiently cover colors in a visible light region and needs to consistently reproduce colors in the visible light region.

[0176] The present application has been made in view of the above problems, and has been made mainly to provide a transmission-type color calibration chart capable of sufficiently covering colors in a visible light region and consistently reproducing colors, and capable of correctly performing color calibration of a photographing apparatus.

[0177] In the present application, it is characterized in that the transmission spectrum of each color bar other than W constituting a color bar group has a peak at a desired interval in the visible light region and is uniformly arranged. That is, as shown in FIG. 1, in the color chart of the present application, the peak wavelength of the transmission spectrum 22(R) of the R color bar is in the range of 600 nm to 680 nm, the peak wavelength of the transmission spectrum 22(G) of the G color bar is in the range of 495 nm to 570 nm, and the peak wavelength of the transmission spectrum 22(B) of the B color bar is in the range of 430 nm to 490 nm. Figure 2

[0178] In addition, the peak wavelength of the transmission spectrum 22(Ye) of the Ye color bar is in the range of 540 nm to 595 nm, and the peak wavelength of the transmission spectrum 22(Cy) of the Cy color bar is in the range of 470 nm to 515 nm.

[0179] According to the present application, the 5 color bars other than white have the peak wavelength of the transmission spectrum at a predetermined position, and thus it is possible to cover colors in the visible light region and consistently reproduce colors in the visible light region by the minimum of 6 colors required to contain desired pointer colors in the xy chromaticity diagram. Thus, it is possible to correctly perform color calibration of a photographing apparatus using the color chart of the present application.

[0180] In the present application, the visible light region refers to a wavelength region of 380 nm to 780 nm. The wavelength range defined in the present specification, which is indicated as "in the range of Anm to Bnm (A and B are numerical values)", can be understood as "A nm or more and B nm or less (A and B are numerical values)".

[0181] In addition, in the present application, the "xy chromaticity diagram" refers to an xy chromaticity diagram of the CIE1931-XYZ colorimetric system.

[0182] In the following description, "separation of the peak top" means suppression of overlapping of the peak top of the transmission spectrum of each color. In addition, "separation of the spectrum" means suppression of color mixing that occurs due to repetition of the transmission spectrum of a plurality of colors.

[0183] ​A color chart of the present mode will be described with reference to the drawings. Figure 1 is a schematic plan view showing an example of a color chart of the present mode. Further, Figure 2 is an image chart showing an example of a spectral transmission spectrum shown in the color chart of the present mode, Figure 3 is an xy chromaticity diagram showing a color gamut of the color chart of the present mode.

[0184] The color chart 100 of the present mode has a transparent substrate 1 and a color bar group 2 formed on the transparent substrate 1. A color bar holding frame 4 is provided on the outer periphery of the color bar group 2.

[0185] The color bar group 2 is composed of at least six color bars of a red color bar 12R, a green color bar 12G, a blue color bar 12B, a yellow color bar 12Ye, a cyan color bar 12Cy, and a white color bar 12W arranged in a pattern in different orders. The white color bar 12W is normally transparent. A light shielding portion 3 is provided around each color bar on the transparent substrate 1.

[0186] The color chart 100 of the present mode has a spectral transmission spectrum characteristic as shown in Figure 2 , has a color gamut LI surrounded by a pentagon formed by connecting each coordinate of the five colors except W on the xy chromaticity diagram with a straight line as shown in Figure 3 . Further, Figure 3 L2 in indicates a color gamut surrounded by a triangle formed by connecting each coordinate of the three colors of R, G, and B on the xy chromaticity diagram. The same applies hereafter.

[0187] With regard to the color chart of the present mode, light incident from the back is spectrally transmitted according to the selective transmissivity of the five color bars except W constituting the color bar group, and the transmission spectrum of each color of the B color bar, the Cy color bar, the G color bar, the Ye color bar, and the R color bar appears in order from the short wavelength side in the visible light region.

[0188] In the present mode, it is characterized that the transmission spectrum of each color bar except W constituting the color bar group has a peak value at a desired interval in the visible light region and is uniformly arranged. That is, in the color chart of the present mode, the peak wavelength of the transmission spectrum 22(R) of the R color bar is in the range of 600 nm to 680 nm, the peak wavelength of the transmission spectrum 22(G) of the G color bar is in the range of 495 nm to 570 nm, and the peak wavelength of the transmission spectrum 22(B) of the B color bar is in the range of 430 nm to 490 nm as shown in Figure 2 .

[0189] Further, the peak wavelength of the transmission spectrum 22(Ye) of the Ye color bar is in the range of 540 nm to 595 nm, and the peak wavelength of the transmission spectrum 22(Cy) of the Cy color bar is in the range of 470 nm to 515 nm.

[0190] Further, the distribution of the chromaticity coordinates of the 5-color bars other than W in the color chart of the present mode on the xy chromaticity diagram is as shown in Figure 3 The color chart of the present mode can reproduce and calibrate the colors of the pointer colors included in the color gamut LI on the xy chromaticity diagram enclosed by the pentagon formed by connecting the coordinates of the 5 colors with straight lines.

[0191] The chromaticity coordinates of the color bars on the xy chromaticity diagram when the spectral distribution of the color bars is P(λ), the color matching functions in the XYZ colorimetric system adopted by CIE in 1931 are x_(λ), y_(λ), z_(λ), and the spectral distribution of the light source is S_(λ) are calculated by

[0192]

[0193] the above equation is prescribed by JIS Z8701.

[0194] Further, here, the spectral distribution of the light source S_(λ) is based on the D65 light source. The white color in the above equation is set as the white color coordinate of D65, and the white color indicated by the above white color coordinate is referred to as "the white color of D65" in the present specification. Figure 3

[0195] According to the present mode, the 5-color bars other than white have the peak wavelength of the transmission spectrum at a predetermined position, so that the colors in the visible light region can be encompassed by the minimum of 6 colors required in the xy chromaticity diagram in order to include the desired pointer colors, and the colors in the visible light region can be reproduced consistently. Thus, the color calibration of the photographic equipment can be performed correctly using the color chart of the present mode.

[0196] Hereinafter, the structures of the color chart of the present mode will be described.

[0197] A. Color bar group

[0198] The color bar group of the present mode is formed on a transparent substrate.

[0199] The above color bar group is formed of at least 6 color bars of R, G, B, Ye, Cy, and W arranged in a pattern in different orders.

[0200] Further, the "5 colors other than W" means 5 colors of R, G, B, Ye, and Cy. In the present specification, the "5-color bar" means the color bar of the above 5 colors unless otherwise specified. Further, the "6-color bar" means the color bar of the total of 6 colors of the above 5 colors and W unless otherwise specified. The same applies to the case where other color bars are included as described later.

[0201] 1. Transmission spectrum ​

[0202] The transmission spectrum of the 5-color stripe of W has a peak wavelength at a predetermined position.

[0203] In the spectral transmission spectrum of the color chart of the present embodiment, the transmission spectrum of each color stripe appears uniformly, respectively, so that a desired wavelength region within the visible light region is captured. Furthermore, adjacent transmission spectra overlap partially, and the spectral transmission spectrum of the color chart is able to capture the entire visible light region.

[0204] (1) Method of specifying peak wavelength

[0205] The transmission spectrum of the 5-color stripe of W has a peak wavelength or a reference wavelength depending on the waveform. That is, the transmission spectrum of the 5-color stripe of W exhibits a sawtooth waveform as shown in Figure 4 (a), and has a peak wavelength λ P at which the transmission rate is high. As for the peak wavelength λ P , the maximum value T max of the measured transmission rate of the transmission spectrum is determined as shown in Figure 4 (a), and the wavelengths λ1 and λ2 at which the relative transmission rate is 50% when the transmission rate at the maximum value T max is set to 100% are determined, and the peak wavelength λ P is set to the center wavelength of the wavelength region from the wavelength λ1 to the wavelength λ2. Furthermore, the measured transmission rate T at the peak wavelength λ P is set to "transmission rate at peak wavelength (hereinafter, sometimes referred to as peak transmission rate)".

[0206] In addition, "transmission rate" simply means the measured transmission rate, and "relative transmission rate" means the measured transmission rate at a specific wavelength converted to a transmission rate of 100%.

[0207] The transmission spectrum of each color stripe was obtained by measuring the transmission rate in the visible light region of 380 nm to 780 nm using an Olympus Corporation Micro Spectrophotometer OSP-SP200 with the achromatic (transparent) white color stripe as a background. Furthermore, when calculating each color coordinate on the xy chromaticity diagram, data of a known D65 light source spectrum was used.

[0208] Hereinafter, the same applies to any color stripe other than the above-described 5 colors.

[0209] (2) Spectral characteristics of each color stripe

[0210] Hereinafter, the spectral characteristics of each color stripe constituting the color stripe group will be described.

[0211] (a) Red (R) color stripe

[0212] The R color stripe has selective transmission properties with respect to red light in the light source.

[0213] The peak wavelength of the transmission spectrum of the R color bar can be in the range of 600 nm to 680 nm, and is preferably in the range of 610 nm to 680 nm, and particularly preferably in the range of 625 nm to 680 nm. This is because, in the case where the peak wavelength is not in the above range, the separation of the peak top is not clear when the transmission spectra of the five colors other than W are uniformly arranged, and it is difficult to achieve an interpolation color effect.

[0214] Further, the half width of the transmission spectrum of the R color bar is preferably 120 nm or less, and is particularly preferably in the range of 20 nm to 100 nm, and particularly preferably in the range of 30 nm to 80 nm. Further, the lower limit of the half width can be 10 nm.

[0215] This is because, in the case where the half width is not in the above range, there is a possibility that a red region of the color gamut of the color chart of the present mode cannot be sufficiently expanded, and the color reproducibility is reduced, because the transmission spectrum of the R color bar has a repeated region with the transmission spectrum of another color bar.

[0216] Further, the half width of the transmission spectrum refers to the interval of the wavelengths at which the half of the peak transmittance of the transmission spectrum is obtained, and can be calculated from the measurement results obtained in the measurement of the transmission spectrum described above (using an Olympus Corporation micro spectrometer OSP-SP200). The same applies to the other color bars.

[0217] Further, the transmission spectrum of the R color bar is preferably the same as the transmission spectrum of red color defined under the BT.2020 standard. That is, the peak wavelength of the transmission spectrum of the R color bar is preferably in the range of 620 nm to 680 nm, and the half width is 100 nm or less, and more preferably the peak wavelength is in the range of 630 nm to 670 nm, and the half width is 80 nm or less, and further preferably the peak wavelength is in the range of 630 nm to 660 nm, and the half width is 70 nm or less.

[0218] This is because, by setting the peak wavelength and the half width of the transmission spectrum of the R color bar in the above range, the coordinates of the R color bar on the xy chromaticity diagram can be made to coincide with or approach the coordinates of red color defined under the BT.2020 standard, and the red region of the color gamut of the color chart of the present mode can be expanded up to the red region defined under the BT.2020 standard.

[0219] (b) Green (G) color bar

[0220] The G color bar has selective transmittance to green light in the light source.

[0221] The peak wavelength of the transmission spectrum of the G color bar can be in the range of 495 nm to 570 nm, and is preferably in the range of 505 nm to 550 nm, and particularly preferably in the range of 510 nm to 540 nm. This is because, if the peak wavelength is not in the above range, the separation of the peak top is not clear when the transmission spectra of the five colors excluding W are uniformly arranged, and it is difficult to achieve an interpolation color effect.

[0222] Further, as the half-width of the transmission spectrum of the G color bar, it is only necessary that the transmission spectrum of the G color bar can represent a waveform that is partially repeated with the transmission spectrum of the Cy color bar and the transmission spectrum of the Ye color bar. Specifically, the half-width is preferably 100 nm or less, and is particularly preferably in the range of 20 nm to 80 nm, and particularly preferably in the range of 30 nm to 60 nm. Further, the lower limit of the half-width can be 10 nm.

[0223] This is because, if the half-width is not in the above range, the transmission spectrum of the G color bar also has a repeated region with the transmission spectrum of the other color bars, and there is a possibility that the green region of the color gamut of the color chart of the present embodiment cannot be sufficiently expanded, and the color reproducibility is reduced.

[0224] Further, the transmission spectrum of the G color bar is preferably the same as the transmission spectrum of green under the BT.2020 standard. That is, the peak wavelength of the transmission spectrum of the G color bar is preferably in the range of 510 nm to 540 nm, and the half-width is 80 nm or less, and more preferably the peak wavelength is in the range of 515 nm to 538 nm, and the half-width is 60 nm or less, and further preferably the peak wavelength is in the range of 520 nm to 535 nm, and the half-width is 40 nm or less.

[0225] This is because, by setting the peak wavelength and the half-width of the transmission spectrum of the G color bar in the above range, the coordinates of the G color bar on the xy chromaticity diagram can be made to coincide with or approach the coordinates of green under the BT.2020 standard, and the green region of the color gamut of the color chart of the present embodiment can be expanded to the green region under the BT.2020 standard.

[0226] (c) Blue (B) color bar

[0227] The B color bar has selective transmission of blue light in a light source.

[0228] The peak wavelength of the transmission spectrum of the B color bar can be in the range of 430 nm to 490 nm, and is preferably in the range of 435 nm to 475 nm, and particularly preferably in the range of 450 nm to 470 nm. This is because, if the peak wavelength is not in the above range, the separation of the peak top is not clear when the transmission spectra of the five colors excluding W are uniformly arranged, and it is difficult to achieve an interpolation color effect.

[0229] Further, as the half-width of the transmission spectrum of the B color bar, it is only necessary that the transmission spectrum of the B color bar can express a part of the transmission spectrum of the Cy color bar in a repeated waveform. Specifically, it is preferable that the above half-width be 100 nm or less, particularly preferably in the range of 20 nm to 85 nm, and especially preferably in the range of 25 nm to 55 nm. Further, as the lower limit of the above half-width, 10 nm can be set.

[0230] This is because, in the case where the above half-width is not in the above range, there is a possibility that a blue region of the color gamut of the color chart of the present mode cannot be sufficiently expanded, and a possibility that the color reproducibility is reduced, because the transmission spectrum of the B color bar has a repeated region with the transmission spectrum of another color bar.

[0231] Further, it is preferable that the transmission spectrum of the B color bar be the same as the transmission spectrum of blue prescribed under the BT.2020 standard. That is, it is preferable that the peak wavelength of the transmission spectrum of the B color bar be in the range of 450 nm to 472 nm, and the half-width be 80 nm or less, more preferably the peak wavelength be in the range of 455 nm to 470 nm, and the half-width be 60 nm or less, and further preferably the peak wavelength be in the range of 458 nm to 468 nm, and the half-width be 40 nm or less.

[0232] This is because, by setting the peak wavelength and the half-width of the transmission spectrum of the R color bar in the above range, the coordinates of the R color bar on the xy chromaticity diagram coincide with or approach the coordinates of blue prescribed under the BT.2020 standard, and it is possible to expand the blue region of the color gamut of the color chart of the present mode up to the blue region prescribed under the BT.2020 standard.

[0233] (d) Yellow (Ye) color bar

[0234] The Ye color bar has selective transmission properties with respect to short-wavelength light and long-wavelength light in a light source.

[0235] Further, the transmission spectrum of the Ye color bar is positioned between the transmission spectrum of the G color bar and the transmission spectrum of the R color bar.

[0236] The peak wavelength of the transmission spectrum of the Ye color bar can be in the range of 540 nm to 595 nm, particularly preferably in the range of 545 nm to 585 nm, and especially preferably in the range of 560 nm to 575 nm. This is because, in the case where the above peak wavelength is not in the above range, the separation of the peaks is not clear when the transmission spectra of the 5 colors other than W are arranged symmetrically, and it is difficult to achieve an interpolation color effect.

[0237] The half-width of the transmission spectrum of the Ye color stripe is preferably 180 nm or less, more preferably 20 to 170 nm, and particularly preferably 30 to 70 nm. The lower limit of the half-width is preferably 10 nm.

[0238] This is because, in the case where the half-width is not within the above range, the overlapping region with the transmission spectrum of another color stripe increases.

[0239] (e) Cyan (Cy) color stripe

[0240] The Cy color stripe has selective transmission properties with respect to short-wavelength light and mid-wavelength light in a light source.

[0241] The transmission spectrum of the Cy color stripe is positioned between the transmission spectrum of the G color stripe and the transmission spectrum of the B color stripe.

[0242] The peak wavelength of the transmission spectrum of the Cy color stripe is preferably within the range of 470 to 515 nm, more preferably within the range of 480 to 505 nm, and particularly preferably within the range of 490 to 500 nm. This is because, in the case where the peak wavelength of the transmission spectrum of the Cy color stripe is not within the above range, the separation of the peaks is not clear when the transmission spectra of the five colors other than W are arranged uniformly, and it is difficult to achieve an interpolation color effect.

[0243] The half-width of the transmission spectrum of the Cy color stripe is preferably 150 nm or less, more preferably 20 to 130 nm, and particularly preferably 30 to 80 nm. The lower limit of the half-width is preferably 10 nm.

[0244] This is because, in the case where the half-width is not within the above range, the overlapping region with the transmission spectrum of another color stripe increases, and particularly with respect to B and G, although the color coordinates on the xy chromaticity diagram are different, it is difficult to achieve spectral separation in the transmission spectrum.

[0245] (f) White (W) color stripe

[0246] The W color stripe is colorless and has light-transmitting properties, and is used as a blank. The W color stripe can be used as a transparent substrate as described later.

[0247] Further, since the brightness adjustment is possible, a W color bar (e.g., ND filter, etc.) designed to uniformly absorb light in the range visible to the eye, which is achromatic, can be arranged on the transparent substrate, and can be used as the transparent substrate.

[0248] (g) Arbitrary color bar

[0249] It is preferable that the color bar group include, in addition to the above-described 6-color color bars, 2-color color bars of violet (V) and near-infrared (NIR) having predetermined transmission spectra. The V color bar and the NIR color bar, which indicate predetermined transmission spectra, are included in addition to the 5-color color bars excluding W, and thus the color chart of the present mode can sufficiently encompass the colors in the visible light region and can reproduce the colors in the visible light region as well. Thus, the accuracy of color calibration of a photographing apparatus using the color chart of the present mode can be improved.

[0250] Further, it is preferable that the color bar group include, in addition to the above-described 6-color color bars, an orange (O) color bar having a predetermined transmission spectrum. The color chart of the present mode further includes the O color bar, which indicates a predetermined transmission spectrum, and thus can compensate for the intermediate colors between yellow and red, which have a wide interval of peak wavelengths, and can evenly and sufficiently encompass the colors in the visible light region, and thus can perform more refined and high-accuracy color calibration.

[0251] The color bar group can be composed of a total of 8 colors including the above-described 6-color color bars and 2 colors of V color bar and NIR color bar having predetermined transmission spectra, or can be composed of a total of 7 colors including the above-described 6-color color bars and the O color bar. Alternatively, it can be composed of a total of 9 colors including the above-described 6-color color bars and 3 colors of V color bar, NIR color bar, and O color bar.

[0252] Figure 5 is a schematic plan view showing another example of the color chart of the present mode, and has a color bar group 2 in which a total of 9 color bars of 6-color color bars 12R, 12G, 12B, 12Ye, 12Cy, 12W shown in Figure 1 are arranged in a pattern in different orders. Figure 5 The color chart shown in Figure 6 has the spectral transmission spectrum characteristics shown in

[0253] Further, Figure 5 The chromaticity coordinates of the 8-color color bars excluding W of the color chart shown in Figure 7 are distributed on an xy chromaticity diagram as shown in

[0254] In addition, in the case where the color bar group is composed of a total of 8 colors including the 6-color color bars described above and a V color bar and a NIR color bar having predetermined transmission spectra, the color chart of the present mode is configured to Figure 6 The spectral transmission spectrum shown is the spectral transmission spectrum excluding the transmission spectrum 22 (O) of the O color bar.

[0255] In addition, in the case where the color bar group is composed of a total of 7 colors including the 6-color color bars described above and an O color bar having a predetermined transmission spectrum, the color chart of the present mode is configured to Figure 6 The spectral transmission spectrum shown is the spectral transmission spectrum excluding the transmission spectrum 22 (NIR) of the NIR color bar and the transmission spectrum 22 (V) of the V color bar.

[0256] It is preferable that the color bar group include a magenta (Mg) color bar in addition to the 6-color color bars described above. White is obtained by mixing R and Cy, or B and Ye, which are in symmetrical positions (belonging to a complementary color relationship) with respect to the W coordinate on the xy chromaticity diagram, but can also be obtained by mixing G and Mg, which have a complementary color relationship, at an appropriate ratio. Thus, the color chart of the present mode is capable of more accurately performing calibration of white by adjusting the same white using three references that utilize a complementary color relationship.

[0257] The color bar group can be composed of a total of 7 colors including the 6-color color bars described above and the Mg color bar, or can be composed of a total of 9 colors including the 6-color color bars described above, the 2 colors of the V color bar and the NIR color bar having predetermined transmission spectra, and the Mg color bar. In addition, it can be composed of a total of 8 colors including the 6-color color bars described above and the O color bar and the Mg color bar having predetermined transmission spectra.

[0258] In addition, the color bar group can be composed of a total of 10 colors including the 6-color color bars described above, the 3 colors of the V color bar, the NIR color bar, and the O color bar having predetermined transmission spectra, and the Mg color bar.

[0259] Figure 8 is a schematic plan view showing another example of the color chart of the present mode, and has a color bar group 2 composed of a total of 10 color bars arranged in a pattern in a different order from that shown in Figure 5 a total of 10 color bars including the 9-color color bars 12R, 12G, 12B, 12Ye, 12Cy, 12W, 12V, 12NIR, 12O shown in the drawing and a magenta color bar 12Mg. Figure 8 The color chart shown in the drawing has Figure 9 the spectral transmission spectrum characteristics shown in the drawing.

[0260] In addition, Figure 8The color chart shown, excluding W, has its chromaticity coordinates for the 9-color bar on the xy chromaticity diagram as follows: Figure 7 With that distribution, the chromaticity coordinates of Mg are contained within the color gamut L3, which is formed by connecting the coordinates of the eight colors mentioned above, excluding Mg, with straight lines.

[0261] Furthermore, when the aforementioned color bar group consists of a total of 7 colors, including the aforementioned 6-color bar and the Mg color bar, the color chart representation of this method... Figure 9 The spectral transmission spectra shown include the spectral transmission spectra of the NIR color bar 22 (NIR), the V color bar 22 (V), and the O color bar 22 (O).

[0262] The following describes the color bars that can be included in the color charts of this method.

[0263] (i) Purple (V) stripe

[0264] Preferably, the transmission spectrum of the V color bar has selective transmittance of light on the shorter wavelength side of the short-wavelength light in the light source than blue light, thus being closer to the shorter wavelength side than the transmission spectrum of the B color bar, and partially repeating the transmission spectrum of the B color bar.

[0265] In addition to the transmission spectrum of the V-colored stripe Figure 6 In addition to the mountain-shaped waveform shown, sometimes as Figure 4 As shown in (b), a waveform with a transmittance of more than a predetermined transmittance below a certain wavelength will not exhibit a mountain-shaped waveform. Therefore, regarding the transmission spectrum of the V-color bar, as... Figure 4 As shown in (b), with a wavelength of 415 nm as the reference wavelength λ s , the reference wavelength λ s The measured transmittance T is set as "the transmittance at the reference wavelength (hereinafter, sometimes referred to as the reference transmittance)".

[0266] The transmission spectrum of the V-colored stripe uses 415nm as the reference wavelength λ. s Preferably, the reference wavelength λ s The wavelength (relative wavelength) λ3 at which the relative transmittance becomes 50% when the transmittance is set to 100% is in the range of 435nm to 465nm, especially preferably in the range of 435nm to 460nm, and particularly preferably in the range of 440nm to 455nm.

[0267] By setting the relative wavelength within the aforementioned range, the waveform expansion of the spectrum in wavelength regions longer than the reference wavelength is suppressed, allowing the transmission spectrum of the B color bar to repeat within the desired wavelength region. Thus, the transmission spectrum of the V color bar can maintain peak separation from the transmission spectrum of the B color bar while also maintaining peak repetition itself, enabling evaluation without missing the shorter wavelength side of the visible light region.

[0268] Preferably, the transmission spectrum of the V-color bar represents a transmittance of a predetermined value or higher in a wavelength region shorter than the aforementioned reference wavelength. "Wavelength region shorter than the reference wavelength" refers to a wavelength region greater than or equal to 380 nm, which is the lower limit of the visible light region, and less than 415 nm, the reference wavelength. Specifically, regarding the transmittance of the V-color bar representing a predetermined value or higher in a wavelength region shorter than the reference wavelength, it is preferable that the relative transmittance in this region, when the reference transmittance is set to 100%, is 5% or higher, more preferably 8% or higher, and even more preferably 10% or higher. This is because it enables the reproduction of the color of the V-color bar in a wavelength region shorter than the reference wavelength.

[0269] Regarding the transmission spectrum of the V color bar, it is preferable that the relative transmittance at wavelengths above 490 nm, when the reference transmittance is set to 100%, is less than 5%, particularly preferably less than 3%, and especially preferably less than 1%. This is because if the relative transmittance of the V color bar in the aforementioned wavelength region exceeds the above range, then in the spectroscopic transmission spectrum, not only does spectral separation from the transmission spectra of other color bars become difficult, but the reproducibility of the color coordinates in the xy chromaticity diagram is also highly likely to become extremely difficult.

[0270] (ii) Near-infrared (NIR) color bars

[0271] The preferred NIR color bar has a transmission spectrum that selectively transmits light from the side of the long wavelength light in the light source that is longer than the red light. As a result, its transmission spectrum is closer to the shorter wavelength side than that of the R color bar, and part of it overlaps with the transmission spectrum of the B color bar.

[0272] The transmission spectrum of NIR color bars is as follows: Figure 4 As shown in (c), sometimes a waveform with a transmittance exceeding a predetermined transmittance above a certain wavelength will not exhibit a mountain-shaped waveform. Therefore, regarding the transmission spectrum of NIR color bars, as... Figure 4 As shown in (c), the wavelength 730nm is set as the reference wavelength λ. t , the reference wavelength λ t The measured transmittance T is set as "the transmittance at the reference wavelength (hereinafter, sometimes referred to as the reference transmittance)".

[0273] Regarding the transmission spectrum of NIR color bars, it is preferable to set 730nm as the reference wavelength λ. t The aforementioned reference wavelength λ t The wavelength (relative wavelength) λ4 with a relative transmittance of 50% when the transmittance is set to 100% is in the range of 630nm to 730nm, particularly preferably in the range of 640nm to 710nm, and especially preferably in the range of 650nm to 700nm.

[0274] By setting the relative wavelength within the above range, the spread of the waveform of the spectrum in the wavelength region shorter than the reference wavelength is suppressed, and the transmission spectrum of the NIR color bar can be repeated in the desired wavelength region as the transmission spectrum of the R color bar. Thus, the transmission spectrum of the NIR color bar can maintain the separation of the peak tops while also maintaining the repetition of the peak values themselves, and thus evaluation can be performed without missing the long-wavelength side of the visible light region.

[0275] Further, it is preferable that the transmission spectrum of the NIR color bar exhibit a transmittance of at least a certain value in the wavelength region longer than the reference wavelength. The "wavelength region longer than the reference wavelength" refers to a wavelength region greater than 730 nm, which is the reference wavelength, and less than or equal to 780 nm, which is the upper limit of the visible light region. With regard to the transmission spectrum of the NIR color bar exhibiting a transmittance of at least a certain value in the wavelength region longer than the reference wavelength, specifically, it is preferable that the relative transmittance in the wavelength region longer than the reference wavelength when the reference transmittance is set to 100% be at least 30%, more preferably at least 50%, and further preferably at least 80%. This is because the color reproduction of the NIR color bar in the wavelength region longer than the reference wavelength can be performed.

[0276] With regard to the transmission spectrum of the NIR color bar, it is preferable that the relative transmittance at a wavelength of 620 nm or less be 5% or less, particularly preferably 2% or less, and especially preferably 1% or less when the reference transmittance is set to 100%. This is because, if the relative transmittance of the transmission spectrum of the NIR color bar in the above wavelength region exceeds the above range, not only does the spectral separation from the transmission spectrum of the other color bars become difficult on the spectroscopic transmission spectrum, but also the reproducibility with respect to the color coordinates in the xy chromaticity diagram becomes extremely low.

[0277] (iii) Orange (O) color bar

[0278] It is preferable that the transmission spectrum of the O color bar have selective transmissivity with respect to light on the short-wavelength side of the long-wavelength light in the light source than the red light, and thus be positioned between the transmission spectrum of the Ye color bar and the transmission spectrum of the R color bar.

[0279] Since the interval of the peak wavelengths between the transmission spectrum of the Ye color bar and the transmission spectrum of the R color bar is wide, by positioning the peak wavelength of the transmission spectrum of the O color bar at the above position, the intermediate color between Ye and R can be compensated for, and the above intermediate color can be reproduced.

[0280] The peak wavelength of the transmittance spectrum of the O color bar is preferably in the range of 575 nm to 620 nm, more preferably in the range of 580 nm to 615 nm, and particularly preferably in the range of 585 nm to 610 nm. This is because, if the peak wavelength is not in the above range, the separation of the peak top is not clear when the transmittance spectra of the 8 colors other than W are arranged uniformly, and it is difficult to achieve the interpolation color effect.

[0281] The half-width of the transmittance spectrum of the O color bar is only required to be such that the transmittance spectrum of the O color bar can be represented by a waveform that is partially repeated with the transmittance spectrum of the Ye color bar and the transmittance spectrum of the R color bar. Specifically, the half-width is preferably 120 nm or less, more preferably in the range of 20 nm to 100 nm, and particularly preferably in the range of 20 nm to 70 nm. Furthermore, the lower limit of the half-width can be set to 10 nm.

[0282] This is because, if the half-width is not in the above range, the overlapping region with the transmittance spectrum of the other color bars increases, and particularly although the colors of Ye, R, and NIR are different in the color coordinates of the xy chromaticity diagram, it is difficult to perform spectral separation in the spectral transmittance.

[0283] With respect to the transmittance spectrum of the O color bar, the relative transmittance at a wavelength at which the peak transmittance is 100% is preferably 10% or less, more preferably 5% or less, and particularly preferably 3% or less in the range of 550 nm or less and 680 nm or more.

[0284] This is because, if the relative transmittance of the transmittance spectrum of the O color bar in the above wavelength region exceeds the above range, the overlapping region with the transmittance spectrum of the other color bars increases, and there is a possibility that it is difficult to perform spectral separation in the spectral transmittance. Furthermore, the color reproduction accuracy of O of the color chart of the present embodiment can sometimes decrease.

[0285] (iv) Magenta (Mg) Color Bar

[0286] The color bar group preferably includes a magenta (Mg) color bar.

[0287] On the xy chromaticity diagram based on the color chart of the present embodiment, the position of the W coordinate can be determined from the positional relationship between the R coordinate and the Cy coordinate and the positional relationship between the B coordinate and the Ye coordinate, and by further considering the positional relationship between the G coordinate and the Mg coordinate, the position of the W coordinate of D65, which is a reference color, can be more correctly determined. In particular, it has the advantage that even when a light source other than the D65 light source is used, the position of the W (65) coordinate can be correctly adjusted by using the color chart of the present embodiment including the Mg color bar.

[0288] The Mg color bar has selective transmittance with respect to red light and blue light in a light source.

[0289] The transmission spectrum 22 (Mg) of the Mg color bar has a first transmission peak (hereinafter, referred to as a first peak wavelength) near the peak wavelength of the transmission spectrum 22 (R) of the R color bar and a second transmission peak (hereinafter, referred to as a second peak wavelength) near the peak wavelength of the transmission spectrum 22 (B) of the B color bar as shown in FIG. 2. Figure 9

[0290] With respect to the transmission spectrum of the Mg color bar, it is preferable that the first peak wavelength be in the range of 567 nm to 780 nm, particularly preferably in the range of 590 nm to 710 nm, and more particularly preferably in the range of 610 nm to 680 nm.

[0291] Further, it is preferable that the second peak wavelength be in the range of 380 nm to 495 nm, particularly preferably in the range of 400 nm to 485 nm, and more particularly preferably in the range of 430 nm to 470 nm. This is because, in the case where each of the peak wavelengths of the transmission spectrum of the Mg color bar is not in the above range, it is difficult to obtain a white color as D65 regardless of the mixing ratio when white color is reproduced by mixing green color having a complementary relationship with magenta color. Further, it is also because it is difficult to fall within the specified color coordinates on the xy chromaticity diagram regardless of the half-width of the transmission spectrum of the Mg color bar.

[0292] (h) Others

[0293] In the spectral transmission spectrum of the color chart of the present embodiment, the transmission spectra adjacent to each other in the visible light region are repeated in a part, so that the mixing ratio of each color component included in the mixed color can be correctly determined, and the color reproduction accuracy and the color calibration accuracy of the mixed color can be improved.

[0294] 2. xy Chromaticity Diagram

[0295] Next, the xy chromaticity diagram represented by the color chart of the present embodiment will be described with reference to the color bar group described above. In the color chart of the present embodiment, the chromaticity coordinates of the 5 color bars of W are distributed on the xy chromaticity diagram as shown in FIG. 3. Figure 3

[0296] As the respective coordinates (x, y) of R, G, B, Ye, and Cy on the xy chromaticity diagram shown in FIG. 2, for example, R (0.708, 0.291), G (0.187, 0.751), B (0.137, 0.049), Ye (0.448, 0.538), and Cy (0.141, 0.339) can be set. Figure 3

[0297] ​​​The color chart of this method can reproduce and calibrate the pointer colors contained within the color gamut enclosed by a pentagon formed by connecting the coordinates of the five colors with straight lines on the xy chromaticity diagram. According to this method, the transmission spectrum is represented by the color bars constituting the color bar group, thereby achieving a wider color gamut than that of color charts in the prior art.

[0298] In this method, the coverage rate of the pointer colors contained within the pentagon formed by connecting the coordinates of the five color bars (excluding white) on the xy chromaticity diagram using straight lines is preferably 90.3% or higher, particularly preferably 99.9% or higher, and even more preferably 100%, that is, all pointer colors are preferably contained within the pentagon. By making the pentagon formed by connecting the coordinates of the five colors (excluding white) the color gamut of the color chart of this method, and by ensuring that the color gamut fully encompasses the pointer colors, colors within the visible light region can be fully captured. Therefore, the colors of real objects can be reproduced more accurately, and thus color calibration of the imaging device can be performed with high precision.

[0299] When the color chart of this method includes V color bars, NIR color bars, and O color bars in the aforementioned color bar group, the chromaticity coordinates of the 8 color bars other than W on the xy chromaticity diagram are as follows: Figure 7 As shown in the diagram.

[0300] As Figure 7 The coordinates (x, y) of V, NIR, and O on the xy chromaticity diagram shown can be set as follows: for example, V (0.164, 0.013), NIR (0.724, 0.272), and O (0.647, 0.352).

[0301] The color chart of this method can expand the color gamut on the xy chromaticity diagram according to the number of colors contained in the above color bar group.

[0302] For example, when the aforementioned color bar group consists of a total of 8 colors, including the V color bar and the NIR color bar in addition to the 6-color color bar, the color chart of this method can reproduce and calibrate the pointer colors contained in the color gamut enclosed by the heptagon formed by connecting the coordinates of the 7 colors excluding W on the xy chromaticity diagram with straight lines.

[0303] Furthermore, when the aforementioned color bar group consists of a total of 7 colors, including the O color bar in addition to the 6 color bars, the color chart of this method can reproduce and calibrate the pointer colors contained in the color gamut enclosed by the hexagon formed by connecting the coordinates of the 6 colors excluding W on the xy chromaticity diagram with straight lines.

[0304] Furthermore, when the aforementioned color bar group consists of a total of 9 colors, including V color bar, NIR color bar and O color bar in addition to the 6-color color bar, the color chart of this method can reproduce and calibrate the pointer colors contained in the color gamut enclosed by the octagon formed by connecting the coordinates of the 8 colors excluding W on the xy chromaticity diagram with straight lines.

[0305] Therefore, by increasing the variety of colors that make up the color bar group, a wider color gamut can be achieved.

[0306] When the color bar group is composed of the above 7, 8 or 9 colors, the coverage rate of the pointer colors contained in the polygon formed by connecting the coordinates on the xy chromaticity diagram with straight lines can be the same as the coverage rate of the pointer colors contained in the pentagon based on 5 color bars.

[0307] In the spectral transmission spectrum of the color chart of this method described above, it is preferable that the peak wavelengths and half-widths of the transmission spectra of each color bar of R, G, and B are within a predetermined range. This is because it allows the color chart of this method to have a color gamut defined by a triangle formed by connecting the coordinates of the three primary colors on the xy chromaticity diagram with straight lines. Figure 3 The L2 coordinates are close to the color gamut specified by the coordinates of the three primary colors in the BT.2020 standard, enabling color calibration for shooting equipment that complies with the BT.2020 standard. Furthermore, by assigning various waveform shapes to the transmission spectrum for each color coordinate, the half-width is set to match the position of the peak wavelength of each color in the transmission spectrum, thus ensuring color reproducibility of the transmission spectrum in this method's color chart.

[0308] like Figure 10 As shown, preferably, the coordinates of the Cy color bar on the xy chromaticity diagram (hereinafter referred to as Cy coordinates) are located on the extension of the line connecting the coordinates of the R color bar on the xy chromaticity diagram (R coordinates) and the W (65) coordinates, and are located on the periphery of the color gamut specified under the BT.2020 standard or outside the color gamut. Preferably, the coordinates of the Ye color bar on the xy chromaticity diagram (hereinafter referred to as Ye coordinates) are located on the extension of the line connecting the coordinates of the B color bar on the xy chromaticity diagram (B coordinates) and the W (D65) coordinates, and are located on the periphery of the color gamut specified under the BT.2020 standard or outside the color gamut.

[0309] On the xy chromaticity diagram, the position of the Cy coordinate is located on the extension line of the line connecting the W (D65) coordinate and the R coordinate, and the position of the Ye coordinate is located on the extension line of the line connecting the W (D65) coordinate and the B coordinate, so that each combination of cyan and red, or yellow and blue, which have a complementary color relationship, can be mixed at an appropriate ratio to reproduce white as D65, and high-precision adjustment of white balance can be achieved. In addition, the positions of the Cy coordinate and the Ye coordinate can be located outside the color gamut prescribed under the BT.2020 standard, and thus the color gamut in which color reproduction can be achieved can be expanded by the color chart of the present mode.

[0310] In particular, it is preferable that the Cy coordinate be an intersection point of the extension line of the line connecting the R coordinate and the W coordinate and the side of the above-mentioned triangle, and the Ye coordinate be an intersection point of the extension line of the line connecting the B coordinate and the W coordinate and the side of the above-mentioned triangle.

[0311] On the xy chromaticity diagram, if the Cy coordinate is located on the above-mentioned triangle, it is possible to check whether the cyan represented by the Cy color bar of the color chart of the present mode and the cyan obtained by mixing green and blue at an appropriate mixing ratio are the same, and it is possible to check whether the white obtained by mixing cyan and red is white of D65.

[0312] In addition, with respect to Ye as well, if the Ye coordinate is located on the above-mentioned triangle on the xy chromaticity diagram, it is possible to check whether the yellow represented by the Ye color bar of the color chart of the present mode and the yellow obtained by mixing green and red at an appropriate mixing ratio are the same, and it is possible to check whether the white obtained by mixing yellow and blue is white of D65.

[0313] Thus, the color chart of the present mode can calibrate the same white obtained by mixing cyan and red and yellow and blue at each ratio as white of D65 by locating the Cy coordinate and the Ye coordinate on the xy chromaticity diagram at the above-mentioned positions.

[0314] In addition, in the case where the above-mentioned color bar group includes the Mg color bar, it is preferable that the coordinate (hereinafter referred to as the Mg coordinate) of the Mg color bar on the xy chromaticity diagram be located on the extension line of the line connecting the coordinate (G coordinate) of the above-mentioned G color bar on the xy chromaticity diagram and the above-mentioned W coordinate, and in particular, it is preferable to be located on the above-mentioned extension line and on the side of the above-mentioned triangle.

[0315] On the xy chromaticity diagram, by locating the position of the Mg coordinate on the extension line of the line connecting the W (D65) coordinate and the G coordinate, each combination of red and cyan, blue and yellow, and green and magenta, which have a complementary color relationship, can be mixed at an appropriate ratio to reproduce white as D65. Thus, the color chart of the present mode can more correctly perform calibration of white and adjustment of white balance.

[0316] In the case of including the Mg color bar, the respective color coordinates of the color gamut do not include the Mg coordinate.

[0317] The transmission spectrum of each of the Cy, Ye, and Mg color bars can be designed by appropriately adjusting the position of the peak wavelength and / or the half-width in accordance with the position of each of the coordinates of Cy, Ye, and Mg on the xy chromaticity diagram. Regarding the transmission spectrum of the Cy color bar, in order to adjust the coordinate of Cy, the following position can be used as a standard for the peak position, i.e., the position at which the extension line of the straight line drawn from the color coordinate position of W (D65) to the color coordinate of Cy as a target intersects with the wavelength position along the outer peripheral curve of the xy chromaticity diagram. Further, the half-width of the transmission spectrum of the Cy color bar can be set so as not to interfere with the peak position of the other transmission spectrum adjacent thereto. The smaller the above half-width, the higher the colorimetric accuracy, and the larger the above half-width, the lower the colorimetric accuracy, and thus the half-width can be changed in accordance with the coordinate position of Cy as a target. The same applies to the transmission spectrum of the Ye color bar.

[0318] Further, regarding Mg, on the xy chromaticity diagram, at the peak wavelength position of the B color bar or the V color bar and the peak wavelength position of the R color bar or the NIR color bar, at which a straight line passing through the coordinate of Mg as a target can be drawn, the first peak and the second peak of the transmission spectrum of the Mg color bar can be set, respectively. The half-width of the transmission spectrum of the Mg color bar can be changed in accordance with the coordinate position of Mg as a target.

[0319] 3. Structure of color bars and color bar groups

[0320] Each color bar can be a member that represents a desired transmission spectrum, and for example, a band-pass filter, a dyed substrate can be used.

[0321] A dyed substrate can be formed by a dyeing method, for example, by adding a solution of silver nitrate to a silver salt emulsion in which potassium bromide is prepared in gelatin, applying the above silver salt emulsion to a chip substrate such as a glass plate, stripping silver from the silver salt photographic plate obtained after drying, and dyeing with a dye corresponding to the color of the color bar. Further, a dyed substrate can also be formed by mixing a dye in gelatin (solution) in advance and applying a material set to a predetermined color to a chip substrate such as a glass plate.

[0322] The peak wavelength position of the transmission spectrum of each color bar can be adjusted in accordance with the type of the color bar and / or the method of forming the same when forming each color bar.

[0323] For example, in the case of using a dyed substrate using one type of dye as a color bar, the peak wavelength of the transmission spectrum of the color bar can also be adjusted by adjusting the concentration of the dye.

[0324] Further, if a dyed substrate formed by combining two or more dyes is used as a color bar, by changing the combination ratio of the two dyes, the peak wavelength of the transmission spectrum of the color bar can be adjusted. Specifically, if a G color bar, by combining a yellow dye and a blue dye and forming by a dyeing method, when shifting the peak wavelength to the long wavelength side, increasing the combination ratio of the yellow dye, when shifting the peak wavelength to the short wavelength side, increasing the combination ratio of the blue dye, the peak wavelength position can be adjusted.

[0325] The size of the color bar and the like is not particularly limited and can be appropriately designed to easily exert a desired effect according to the use of the color chart of the present mode.

[0326] In the color bar group, each color bar is arranged in a pattern in a different order. As the arrangement pattern of each color bar, it can be arranged in a linear pattern in a row as exemplified, although not exemplified, it can be arranged in a lattice pattern or a circular shape. Figure 1

[0327] Further, the arrangement order of each color bar is not particularly limited and can be appropriately designed to easily exert a desired effect according to the use of the color chart of the present mode.

[0328] Each color bar can be formed by a method known in the art such as an evaporation method, a dyeing method, a printing method, a transfer method, an inkjet method, and the like. The method of forming a color bar by a dyeing method has been described, and thus the description thereof is omitted here.

[0329] Further, the color bar group can be formed by arranging each color bar formed by the above-described method in a desired pattern on one side of the transparent substrate described later, and sandwiching by the transparent substrate and the cover glass.

[0330] B. Light shielding portion

[0331] The color chart of the present mode generally has a light shielding portion provided on the outer periphery of the color bar group on the transparent substrate described above.

[0332] As the light shielding portion, as long as it has a desired light shielding property, for example, a metal film such as a chromium thin film, a printed layer formed of black ink, and the like can be exemplified.

[0333] As for the method of forming the light shielding portion, a method known in the art can be used according to the material used.

[0334] C. Transparent substrate

[0335] ​As the transparent substrate of the present embodiment, there is no particular limitation as long as it can support the color bar group and the light shielding portion and has a desired light transmittance, and it can be the same as the transparent substrate used for a color chart known in the art. Specifically, an inorganic substrate such as a glass substrate or a resin substrate can be used. The resin substrate can be frame-shaped or sheet-shaped in addition to plate-shaped.

[0336] D. Other

[0337] The color chart of the present embodiment can have a calibration mark, an identification code, a cover glass, a color bar holding frame, a transparent protective sheet with a light shielding portion, and the like in addition to the above-described structure.

[0338] The identification code can be, for example, a code that records information of a test chart and the like. Further, the calibration mark can be a mark that records position information, and it can further function as an identification code that records information of a test chart and the like. These can be provided on the transparent protective sheet with a light shielding portion.

[0339] Further, the color chart of the present embodiment has an IR cut filter. In the case where each color color bar is formed by a dyeing method, on the characteristics of the dye, the transmittance spectrum has a tendency to easily transmit light in the wavelength region of 650 nm or more, and can have a high light transmittance. In particular, the dye used for the Ye, O, and R color bars has a tendency to hardly absorb light on the long wavelength side from around 650 nm. Therefore, in the long wavelength wavelength region, the transmittance spectrum of each color can be repeated.

[0340] In view of this, by removing the IR cut filter of the predetermined region in cooperation with the color bar, it is possible to separate the transmittance spectrum of each color, and it is possible to prevent color mixing.

[0341] The IR cut filter can be selected in consideration of the wavelength region to be blocked in cooperation with the transmittance spectrum characteristics of each color color bar. The IR cut filter can use a device known in the art.

[0342] The color chart of the present embodiment can be designed in size according to the applicable captured image. For example, in the case where the color chart of the present embodiment is used for color evaluation and color correction of an output image of a measurement sample captured by a microscope of a photographic device for pathology, it can be a micro-imaging color chart of a color bar group formed in a size corresponding to the magnification of the objective lens of the microscope.

[0343] Further, in the case where the color chart of the present embodiment is used, for example, for color evaluation and color correction of an output image of a measurement sample captured by a photographic device or the like at a magnification, it can be a macro-imaging color chart of a color bar group formed in a size corresponding to the size of the captured image.

[0344] E. Use

[0345] The color chart of the present embodiment can be used in a wide range of photographic equipment, video equipment, and peripheral equipment that require color calibration. In particular, it can be preferably used in photographic equipment for pathological use.

[0346] The present application is not limited to the above-described embodiments. The above-described embodiments are examples, and solutions having substantially the same structure as that recited in the technical idea of the claims of the present application and achieving the same effects are included in the technical scope of the present application.

[0347] II. Calibration slide glass

[0348] Hereinafter, a calibration slide glass of the present application will be described.

[0349] The calibration slide glass of the present application is characterized by comprising: a calibration pattern having a plurality of calibration pattern chips; a first spacer disposed around the calibration pattern; a pair of protective substrates disposed so as to face each other across the calibration pattern and the first spacer, the area overlapping the calibration pattern in plan view having at least a transmission portion; and a second spacer disposed so as to overlap a part of the calibration pattern chips in plan view, between the first spacer and at least one of the pair of protective substrates, the second spacer having at least an opening portion in the area overlapping the calibration pattern in plan view.

[0350] In recent years, as a device used for sample observation, for example, an observation device provided with a photographic device such as a TV camera or a digital camera is known. As an observation device provided with a photographic device, a microscope provided with a photographic device mounted thereon can be exemplified. In a case where such an observation device provided with a photographic device is used, an effect that the observation result can be recorded, or an effect that information input from the microscope can be directly output to a display or a printer so that the sample can be rapidly evaluated is exerted. Therefore, the observation device provided with a photographic device is preferably used in a wide range of fields such as medical sites.

[0351] An observation device provided with a photographic device generally uses a calibration slide glass. The calibration slide glass has, for example, a function of providing color information serving as a comparison reference when color evaluation or color correction of a sample image photographed by the observation device is performed. For example, Patent Literature 1 discloses a slide glass in which one or more micro-filter groups including two or more color reference micro-filters for taking colors serving as comparison references used in color evaluation and color correction of an image are disposed on one face of a protective substrate, and two or more color reference micro-filters belonging to the same group in the one or more micro-filter groups are respectively disposed so as not to overlap each other on the face of the protective substrate, and respectively have different reference colors.

[0352] However, the sliding glass disclosed in Patent Document 1 has a microfilter disposed on a protective substrate, and the surface of the microfilter is exposed. Therefore, when using an observation device with a shooting device that utilizes this sliding glass, for example, physical contact when wiping away solvents or dirt adhering to the surface of the microfilter can cause adverse conditions such as a decrease in the color concentration of the microfilter or a change in pigmentation.

[0353] To address this problem, the inventors of this invention have manufactured a sliding glass for calibration having a structure in which a calibration pattern chip (micro-filter) is sandwiched between a pair of protective substrates. Specifically, as... Figure 21 As shown, a sliding glass 100' was manufactured, comprising: calibration pattern chips 10a and 10b; a pair of protective substrates 20a and 20b, configured to face each other across the calibration pattern chips 10a and 10b, wherein the area overlapping the calibration pattern chips 10a and 10b when viewed from above has at least a transparent portion 21; and a first spacer 30, held between the pair of protective substrates 20a and 20b, configured to fill the area without the calibration pattern chips 10a and 10b. As a result, the inventors of the present invention obtained the following insights. That is, as... Figure 21 As shown, by clamping the calibration pattern chip with a pair of protective substrates, it is possible to suppress the adhesion of solvent isotropically to the surface of the calibration pattern chip and physical contact with the calibration pattern chip, thereby suppressing adverse conditions such as a decrease in color concentration or pigment changes in the calibration pattern chip.

[0354] However, in the calibration sliding glass described above, there is a problem that Newton's rings appear in the protective substrate for observing the calibration pattern chip. Therefore, the inventors of the present invention, after repeating the results of various investigations, found that one of the reasons for the appearance of Newton's rings is contact between the calibration pattern chip and the protective substrate.

[0355] The present invention was made in view of the above-mentioned actual situation, and its main objective is to provide a sliding glass for calibration that can suppress the occurrence of Newton's rings.

[0356] The calibration sliding glass of the present invention will be described with reference to the figures.

[0357] Figure 12 This is a schematic top view illustrating an example of the calibration sliding glass of the present invention. Figure 12 As shown, the calibration sliding glass 200 of the present invention has a calibration pattern having a plurality of calibration pattern chips.

[0358] Figure 13 (a) is an amplification of the image. Figure 12 The image shown is an enlarged view of the region R1 enclosed by the white dashed line in the calibration sliding glass 200 of the present invention. Figure 13 (b) is Figure 13An A-A line sectional view of (a), Figure 13 (c) is an enlarged view of the area R2 enclosed by the broken line in (b). Figure 13 (c) is an enlarged view of the area R2 enclosed by the broken line in (b). As shown in (b) and (c), the calibration sliding glass 200 of the present application is characterized by having: a calibration pattern 10 having a plurality of calibration pattern chips 10a, 10b, 10c; a first spacer 30 disposed around the calibration pattern 10; a pair of protective substrates 20a, 20b disposed so as to face each other across the first spacer 30 and the first spacer 30, and having a transmissive portion 21 at least in an area overlapping the calibration pattern 10 in plan view; and a second spacer 40 disposed so as to overlap a portion of the calibration pattern chip in plan view between the first spacer 30 and at least one of the pair of protective substrates 20a, 20b. Further, in the calibration sliding glass 200 of the present application, the second spacer 40 has an opening portion A at least in an area overlapping the calibration pattern 10 in plan view. Figure 13 The calibration sliding glass of the related art has a structure in which a calibration pattern is disposed on a protective substrate. In the calibration sliding glass having such a structure, since the surface of the calibration pattern is exposed, there is a problem that adverse situations such as contamination of the surface of the calibration pattern, reduction of color density due to physical contact, and occurrence of color change, etc. are caused.

[0359] Therefore, the inventors of the present application have produced, for example, a calibration sliding glass having a structure in which a calibration pattern 10 is disposed between a pair of protective substrates 20a, 20b and the surface of the calibration pattern is prevented from being exposed as shown in (b) and (c).

[0360] As a result, it is possible to suppress contamination of the surface of the calibration pattern, reduction of color density, and occurrence of color change, but on the other hand, a new problem has been found that when the calibration pattern is observed across the transmissive portion provided in the protective substrate, Newton's rings appear. Figure 21 As a result of further repeated research by the inventors of the present application, it has been newly found that one of the causes of the occurrence of Newton's rings is contact between the calibration pattern and the protective substrate.

[0361] Therefore, according to the present application, by providing the second spacer with an opening portion at least in an area overlapping the calibration pattern in plan view, it is possible to provide a gap of an amount corresponding to the thickness of the second spacer between the calibration pattern and the transmissive portion of at least one of the protective substrates on which the second spacer is disposed, and thus it is possible to produce a calibration sliding glass in which the occurrence of Newton's rings is suppressed.

[0362]

[0363] ​The calibration sliding glass according to the present application has a gap of a size corresponding to the thickness of the second spacer between the transparent portion of the protective substrate of at least one of the calibration pattern chip and the protective substrate provided with the second spacer. In the calibration sliding glass according to the present application, generally, the surface of the side having the gap becomes the observation side. Therefore, in the case where the gap is provided on both surfaces of the calibration pattern chip, both surfaces of the calibration sliding glass can be used as the observation side, and in the case where the gap is provided on one surface of the calibration pattern chip, the surface of the side where the gap is provided in the calibration sliding glass becomes the observation side.

[0364] The size of the gap is not particularly limited as long as it is a size that can suppress the occurrence of Newton's rings due to contact between the calibration pattern chip and the protective glass, and can be appropriately adjusted according to the design of the calibration sliding glass or the like. For example, the thickness of the gap is a size that can suppress the occurrence of Newton's rings, and corresponds to the thickness of the second spacer described later. The thickness of the gap is, for example, preferably in the range of 10 μm to 250 μm, more preferably in the range of 15 μm to 150 μm, and particularly preferably in the range of 20 μm to 80 μm. In addition, the thickness of the gap is preferably in the range of 1 / 10 to 1 / 2 of the thickness of the second spacer. Figure 13 The symbol t1 shown in (c).

[0365] Hereinafter, each component constituting the calibration sliding glass according to the present application will be described.

[0366] A. Second spacer

[0367] The second spacer in the present application is a component that is provided to overlap with a part of the calibration pattern chip when viewed from above, between the first spacer and at least one of the protective substrate among the pair of protective substrates and the first spacer. Further, the second spacer according to the present application is characterized by having at least an opening portion in the region overlapping with the calibration pattern when viewed from above. In addition, in the present application, for example, as shown in Figure 15 , it can be stacked on the first spacer 30 provided around the calibration pattern. In addition, Figure 15 The description of the second spacer will be described later, and therefore the description thereof is omitted here.

[0368] In the present application, for example, as shown in Figure 14 , Figure 15 It is preferable that the second spacer 40 be provided to overlap with a part of the calibration pattern chip 10 when viewed from above, between the first spacer 30 and the pair of protective substrates 20a, 20b, that is, on both surfaces of the first spacer 30. This is because, in any surface of the calibration sliding glass 200, a gap A can be provided between the protective substrates 20a, 20b and the calibration pattern chip 10, and the occurrence of Newton's rings can be suppressed.

[0369] In the present application, the second spacer is configured to overlap with a portion of the calibration-use pattern chip in plan view, so that when at least an opening portion is formed in a region overlapping with the calibration-use pattern in plan view, i.e., when a gap is provided between the protective substrate and the calibration-use pattern chip, displacement of the calibration-use pattern chip into the region made into a gap or the like is prevented.

[0370] The second spacer of the present application has at least an opening portion in a region overlapping with the calibration-use pattern in plan view. The second spacer of the present application can be configured, for example, in a layered manner so as to overlap with all regions other than the transparent portion of the protective substrate in plan view, or can be configured in a layered manner so as to overlap with at least a portion of the regions other than the transparent portion of the protective substrate, i.e., the regions around the protective substrate, in plan view. In particular, it is preferable that the second spacer be configured to overlap with all regions other than the transparent portion of the protective substrate in plan view. By the second spacer having at least an opening portion in a region overlapping with the calibration-use pattern in plan view, even in the case where a gap is provided between the calibration-use pattern chip and the protective substrate, the reduction in the strength of the calibration-use sliding glass due to the occurrence of a bend, breakage, or the like of the protective substrate by pressure can be suppressed.

[0371] The thickness of the second spacer of the present application can be appropriately adjusted according to the size of the gap to be provided between the calibration-use pattern chip and the protective substrate, and is not particularly limited. As a specific thickness of the second spacer, for example, it is preferable to be in the range of 10 μm to 250 μm, particularly preferably in the range of 15 μm to 150 μm, and especially preferably in the range of 20 μm to 80 μm. By making the thickness of the second spacer in the above range, a gap can be provided to the extent that the occurrence of Newton's rings can be suppressed.

[0372] The material of the second spacer of the present application is not particularly limited as long as it is a material that can be used for the calibration-use sliding glass of the present application and can form at least an opening portion in a region overlapping with the calibration-use pattern in plan view. As a specific material of the second spacer, for example, a material having a predetermined rigidity and being easy to process can be exemplified, such as a metal such as stainless steel, iron-nickel alloy (42 alloy), copper, glass, plastic, and the like. In the present application, it is preferable to use a metal, and more preferably to use stainless steel from the viewpoints of corrosion resistance and processability.

[0373] The forming method of the second spacer of the present application is not particularly limited as long as it is a method capable of forming a second spacer having a desired shape, and can be appropriately selected according to the kind of the material to be used in the second spacer, and the like. In the present application, for example, the second spacer can be formed by punching or etching processing of a metal plate, punching processing or the like through processing of a plastic plate, plastic molding, printing processing, or the like.

[0374] In the present application, as a method of arranging the second spacer between the first spacer and the protective substrate in a manner so as to overlap with a portion of the calibration-use pattern chip when viewed from above, for example, a method in which the second spacer is adhesively arranged between the first spacer and the protective substrate so as to overlap with a portion of the calibration-use pattern chip when viewed from above can be exemplified. Here, in the case where the second spacer is adhesively arranged between the first spacer and the protective substrate, an adhesive is generally used, and in the present application, a liquid adhesive is preferably used. Compared to the case where an adhesive sheet or the like is used, an increase in thickness due to the adhesive can be suppressed, and a high adhesive strength can be obtained. In addition, the specific material used as the adhesive can be the same as the general material, and the description thereof will be omitted.

[0375] In the case where a liquid adhesive is used in the arrangement of the second spacer, it is preferable that a groove for arranging the adhesive be provided on the surface of the second spacer. For example, as shown in Figure 16 , it is preferable that a groove 60 for arranging a liquid adhesive be provided on the surface of the second spacer 40. In particular, it is preferable that a groove be provided on the surface of the second spacer in a region other than the region that overlaps with a portion of the calibration-use pattern chip when viewed from above. By providing a groove on the surface of the second spacer in a region other than the region that overlaps with a portion of the calibration-use pattern chip when viewed from above, positional displacement of the calibration-use pattern chip into a region that becomes a gap or the like can be effectively prevented by the second spacer. In addition, the "surface of the second spacer" herein refers not only to either of the surface that contacts the first spacer or the surface that contacts the protective substrate, but also to both of the surface that contacts the first spacer and the surface that contacts the protective substrate.

[0376] In the case where a groove is provided on the surface of the second spacer, the depth of the groove is preferably a depth that is smaller than the thickness of the second spacer, and a liquid adhesive is arranged in the groove, whereby the second spacer can be sufficiently adhesively arranged between the first spacer and the protective substrate. In this way, the depth of the groove can be appropriately adjusted according to the thickness of the second spacer, and for example, the ratio with respect to the thickness of the second spacer is preferably in the range of 10% to 90%, in particular, in the range of 20% to 80%, and particularly preferably in the range of 30% to 70%. Furthermore, the specific depth of the groove is, for example, preferably in the range of 5 μm to 130 μm, in particular, in the range of 10 μm to 80 μm, and particularly preferably in the range of 10 μm to 40 μm. By the depth of the groove being in the above range, in the case where a liquid adhesive is arranged in the groove, the release groove that can be used as the adhesive can suppress leakage of the adhesive from between the second spacer and the first spacer and the protective substrate. Thus, a calibration-use sliding glass of high quality can be produced. In addition, the depth of the groove corresponds to, for example, the symbol t2 shown in Figure 16 .

[0377] In the case where the surface of the second spacer has a groove, the size and number of the groove are not particularly limited, and are preferably appropriately adjusted in accordance with the shape of the opening portion of the second spacer, the amount of the adhesive used, or the like.

[0378] As a method of forming a groove in the surface of the second spacer, a method capable of forming a groove having a predetermined depth as described above is preferably used, and can be appropriately selected in accordance with the kind of the material used for the second spacer, or the like. For example, a method in which a desired groove is formed by performing a half-etching process on the surface of the second spacer can be used.

[0379] B. First spacer

[0380] The first spacer of the present application is a member disposed around the calibration pattern.

[0381] In the present application, the first spacer is disposed around the calibration pattern, so that the first spacer can fill in the area where the calibration pattern is not present. Therefore, it is possible to fix the calibration pattern chip at a predetermined position, and it is possible to suppress positional displacement of the calibration pattern chip in the length direction of the pair of protective substrates.

[0382] The first spacer of the present application is not particularly limited as long as it can be disposed around the calibration pattern, and can be composed of one layer or a plurality of layers. In the present application, from the viewpoint that it is easy to perform a processing such as etching on the first spacer when the first spacer is formed, for example, as shown in FIG. 1, a structure in which a plurality of thin first spacers 30 are laminated is preferred. In addition, the description of the first spacer will be described later, and thus the description thereof is omitted here. Figure 15 Figure 15

[0383] In the case where the first spacer of the present application is composed of a plurality of layers, the thickness of one layer of the first spacer can be appropriately adjusted in accordance with the material used for the first spacer, or the like. As a specific thickness of one layer of the first spacer, for example, it is preferred to be in the range of 10 μm to 250 μm, particularly preferably in the range of 20 μm to 150 μm, and especially preferably in the range of 50 μm to 150 μm. By making the thickness of one layer of the first spacer be in the above range, it is easy to perform a processing such as etching when the first spacer is formed.

[0384] Further, the total thickness of the first spacer of the present application can be appropriately adjusted in accordance with the thickness of the calibration pattern chip to be described later, and is not particularly limited. For example, it is preferred to be in the range of 0.02 mm to 0.8 mm, particularly preferably in the range of 0.07 mm to 0.6 mm, and especially preferably in the range of 0.1 mm to 0.4 mm. By making the total thickness of the first spacer be in the above range, it is possible to sufficiently fix the calibration pattern chip.

[0385] ​​As the material of the first spacer of the present application, there is no particular limitation as long as it is a material configured around the calibration pattern to fix the calibration pattern chip. As a specific material of the first spacer, for example, a material having a predetermined rigidity and being easy to process is preferable, and a metal such as stainless steel, iron-nickel alloy (42 alloy), copper alloy, glass, plastic, and the like can be exemplified. In the present application, a metal is preferably used, and stainless steel is more preferable from the viewpoints of corrosion resistance and processability.

[0386] As the forming method of the first spacer of the present application, there is no particular limitation as long as it is a method by which a first spacer having a desired shape can be formed, and it can be appropriately selected depending on the kind of the material used in the first spacer and the like. In addition, as for the forming method of the specific first spacer, it can be the same as the forming method of the second spacer described in the above "1. Second spacer" item, and thus the description thereof is omitted here.

[0387] In the present application, as the method of configuring the first spacer around the calibration pattern, for example, a method of adhesively configuring the first spacer at a predetermined position of the protective substrate can be exemplified. Here, in the case of adhering the first spacer to the protective substrate, an adhesive is generally used, and in the present application, a liquid adhesive is preferably used. Compared to the case where an adhesive sheet or the like is used, an increase in thickness caused by the adhesive can be suppressed, and a high adhesive strength can be obtained. In addition, as for the specific material used as the adhesive, it is the same as the general material, and thus the description thereof is omitted here.

[0388] In the case where a liquid adhesive is used in the configuration of the first spacer, it is preferable that a groove for configuring the adhesive is provided on the surface of the first spacer. In addition, the "surface of the first spacer" here includes, in addition to the surface of the first spacer which contacts the protective substrate, the surface of the first spacer which contacts the protective substrate, the surface of the first spacer which contacts the second spacer, and the like in the case where the first spacer is composed of a plurality of layers. As for the depth, size, forming method, and the like of the specific groove, the same as the contents described in the above "1. Second spacer" item can be exemplified, and thus the description thereof is omitted here.

[0389] C. Calibration pattern

[0390] The calibration pattern of the present application is a member having a plurality of calibration pattern chips.

[0391] The calibration pattern of the present application can be appropriately selected according to the use of the calibration slide glass of the present application, and is not particularly limited. For example, in the case where the calibration slide glass of the present application is used in a microscope with a camera, the calibration pattern for the calibration slide glass can be used in the reproducibility evaluation of the measurement image obtained by the microscope with a camera. Thus, as the calibration pattern at this time, for example, a color chart, a gray scale, a resolution chart, a TV test pattern chart, a cross hatch chart, or a combination thereof, or the like can be exemplified.

[0392] The number of the calibration pattern of the present application can be appropriately adjusted according to the number of the objective lens of the microscope with a camera or the like used for the calibration slide glass of the present application. In addition, in the case where the calibration slide glass of the present application is used in a microscope with a camera, the number of the calibration pattern can be appropriately adjusted according to the number of the objective lens of the microscope with a camera. Figure 12 In the calibration slide glass shown in FIG. 1, the calibration pattern is provided at three places.

[0393] The calibration pattern of the present application is designed to change in size in stages and the patterns are similar, for example, as shown in FIG. 2. Figure 12 Specifically, the size of the calibration pattern is designed to be a size that can be accommodated within the field of view measured by the microscope based on the objective lens. In addition, as the shape of the calibration pattern, in addition to the rectangular shape shown in FIG. 2, a square shape, a polygonal shape, a circular shape, or the like can be exemplified. Figure 12

[0394] In addition, the thickness of the calibration pattern of the present application can be appropriately adjusted according to the design of the calibration slide glass of the present application, and is not particularly limited. As the thickness of the calibration pattern, for example, it is preferable to be in the range of 0.02 mm to 0.8 mm, particularly preferably in the range of 0.07 mm to 0.6 mm, and especially preferably in the range of 0.1 mm to 0.4 mm.

[0395] Here, as an example of the calibration pattern of the present application, a color chart is described. The color chart is composed of, for example, red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M) as colors for color evaluation. Thus, at this time, the calibration pattern of the present application is a pattern in which calibration pattern chips of each color for color evaluation are arranged.

[0396] The calibration pattern chip of the present application is designed to change in size in stages and the patterns are similar, for example, as shown in FIG. 3. Figure 13 The colored portion and the transparent portion can be appropriately selected according to the design of the calibration pattern chip of the present application, and are not particularly limited. For example, it is preferable that the colored portion and the transparent portion are arranged in each calibration pattern chip. Specifically, for example, as shown in FIG. 4, the colored portion 10a", 10b" and the transparent portion 10a', 10b' can be included. Figure 13 ​(b) In each of the calibration pattern chips 10a, 10b, the colored portion 10a", 10b" is disposed on the surface of the protective substrate 20a side, and the transparent portion 10a', 10b' is disposed on the surface of the protective substrate 20b side of the colored portion 10a", 10b". By making the disposition of the colored portion and the transparent portion uniform in each of the calibration pattern chips, the following effects can be obtained. That is, the colored portion and the transparent portion have different refractive indexes, and thus when light passes through the calibration pattern chip, there is a tendency for refraction of light to occur at the interface between the colored portion and the transparent portion. In this case, when the disposition of the colored portion and the transparent portion is different in each of the calibration pattern chips, the position of the interface at which refraction of light occurs is different, and thus there is a deviation in the angle of light emitted from each of the calibration pattern chips, and it can be difficult to obtain desired color information in the light receiving portion. On the other hand, as described above, when the disposition of the colored portion and the transparent portion is uniform in each of the calibration pattern chips, the above problem can be suppressed.

[0397] The calibration pattern chip of the present application is obtained, for example, by cutting a color chart formed in a size larger than the size of the calibration pattern chip into a desired size. By producing the calibration pattern chip by this method, generation of uneven color or uneven density is suppressed, and a calibration pattern chip having stable characteristics can be obtained.

[0398] Further, in the color chart, for example, "Standard Color Bar Chart" manufactured by Dai Nippon Printing Co., Ltd. can be used as a reference color of a microscope with a photographing device. In this case, the "Standard Color Bar Chart" is composed of red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M) which are designed in advance, and the effective size is 175 mm x 245 mm.

[0399] Further, the calibration pattern chip of the present application is used as a reference color for evaluating reproducibility of a measurement image obtained by a microscope with a photographing device, and as the reference color at this time, for example, red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M) can be cited. In addition, the colors used for the reference color are not limited to the above-mentioned six colors, and can be appropriately adjusted according to the object observed by the microscope with a photographing device. Specifically, in the case where biological tissue or cells are stained and observed, colors selected from red, green, blue, cyan, magenta, yellow, other system colors, and the like which are colored by a general staining method for biological tissue, i.e., hematoxylin-eosin staining method or other staining methods can be used as the reference color.

[0400] The plurality of calibration pattern chips of the calibration pattern can be arranged in a line, can be arranged in a lattice shape, or can be arranged in a circular shape. In addition, in the case where the plurality of calibration pattern chips are arranged in a line, the line direction can be parallel to the direction of the long side of the calibration pattern chip, or can be parallel to the direction of the short side of the calibration pattern chip. Figure 13In the case of (a), the calibration pattern 10 shows an example in which the calibration pattern chips 10a, 10b are arranged in a 2-row by 5-column lattice. In addition, as shown in Figure 13 As shown in (a) and (b), between the upper-stage calibration pattern chip and the lower-stage calibration pattern chip, there is a blank region 10c having no color transmittance.

[0401] Next, the gray scale of the calibration pattern of the present application is described. The gray scale is a scale in which the reflection density of the gray scale between white and black is changed to express the gradation in stages. Here, the transmissive gray scale is described. The gray scale of the calibration pattern can be configured by arranging the calibration pattern chips of each gradation having different transmittances. As the arrangement, the arrangement can be made in one column or in a lattice so that the transmittance changes in stages. In addition, as the formation method of the gray scale, for example, the sputtering method in which a plurality of metal films having different thicknesses are formed on a support such as glass or a film, the printing method or the inkjet method in which a gradation of a thick and thin level is formed on a support, or the photolithography method in which a dot-shaped coating portion having a different coating area is formed on a support can be cited.

[0402] The calibration pattern of the present application can also have an IR cut filter and / or an ND filter as needed. For example, as shown in Figure 15 In addition, as shown in Figure 15 In the case where the IR cut filter 80 is provided, the second spacer 40 is arranged between the calibration pattern chip 10a, 10b and the IR cut filter 80 so as to overlap with a part of the calibration pattern chip 10 when viewed from above, thereby providing a gap A, and the generation of Newton's rings can be suppressed.

[0403] In the present application, it is preferable that all of the calibration pattern chips which become the same measurement target among the plurality of calibration pattern chips have uniform optical path lengths in the thickness direction. Hereinafter, a detailed description is given.

[0404] First, the calibration slide glass has a function of providing color information as a comparison reference when color evaluation or color correction of a sample image captured by an observation device is performed, for example. Specifically, the plurality of calibration pattern chips constituting the calibration pattern each has color information, and the plurality of calibration pattern chips are measured to provide the color information, and thus color evaluation or color correction of a sample image captured by an observation device can be performed.

[0405] On the other hand, when providing color information, the calibration sliding glass measures multiple calibration pattern chips. Preferably, the measured multiple calibration pattern chips have uniform optical path lengths in the thickness direction. In particular, it is preferable that all calibration pattern chips constituting the calibration pattern have uniform optical path lengths in the thickness direction. Here, the "multiple calibration pattern chips measured at this time" refers to calibration pattern chips measured in a single calibration, meaning multiple calibration pattern chips that are considered to be the same measurement object. For example, if a white calibration pattern chip is used as a reference color, and the color information of pattern chips of other colors is measured, the white calibration pattern chip and the pattern chips of other colors become "the same measurement object." Furthermore, if the calibration pattern includes a gray chip for brightness adjustment in addition to the calibration pattern chip of a predetermined color, and the gray chip and the calibration pattern chip are measured in a single calibration, the gray chip and the calibration pattern chip become "the same measurement object." In addition, if the calibration pattern chip has a resolution chip in addition to the calibration pattern chip with a predetermined color, it is not necessary to measure the resolution chip and the calibration pattern chip in one calibration. Therefore, the resolution chart and the calibration pattern chip can be set as "the same measurement object" as needed.

[0406] The reason for preferring that the optical path lengths of multiple calibration pattern chips for the same measurement object be uniform can be considered as follows. Figure 22 In (a) and (b), the calibration pattern chips include a red calibration pattern chip 10R, a white calibration pattern chip 10W, and a green calibration pattern chip 10G. An IR cutoff color filter 80 is disposed in the area where the red calibration pattern chip 10R and the green calibration pattern chip 10G overlap when viewed from above. Furthermore, regarding... Figure 22 The other structures in (a) and (b) are related to the above. Figure 15 The same applies, therefore the description here is omitted. Furthermore, in Figure 22 In (a) and (b), the first and second isolation components are omitted for the sake of simplification, as are the colored and transparent parts of the calibration pattern chip.

[0407] like Figure 22 As shown in (a), when parallel light is shone onto the calibration sliding glass as a light source h, the parallel light passing through the red calibration pattern chip 10R is received by the light-receiving unit 300R, and the desired color information is measured. Furthermore, parallel light passing through the white calibration pattern chip 10W is received by the light-receiving unit 300W, and the desired color information is measured. Additionally, parallel light passing through the green calibration pattern chip 10G is received by the light-receiving unit 300G, and the desired color information is measured. On the other hand, as... Figure 22(b) shown, when non-parallel light is irradiated to the calibration sliding glass as the light source h, the non-parallel light that has passed through the red calibration pattern chip 10R and the green calibration pattern chip 10G is not received by the light receiving portion 300R and the light receiving portion 300G, and it is sometimes difficult to measure the desired color information.

[0408] Therefore, for example, in the case where the white calibration pattern chip 10W is used as a reference color, and the red calibration pattern chip 10R and the green calibration pattern chip 10G are measured, the measurement result can be deviated, and it can be difficult to perform correct calibration. In addition, as a result, it can be difficult to perform high-precision measurement by the observation device using the calibration sliding glass with the calibration pattern chip.

[0409] In addition, as the above-mentioned "non-parallel light", for example, an imaging optical system such as a microscope can be cited.

[0410] Therefore, the inventors of the present application and the like repeated the results of the review in view of the above-mentioned problems, and obtained the following insight, that is, as Figure 22 (b) shown, when non-parallel light is irradiated to the calibration sliding glass as the light source h, the non-parallel light that has passed through the red calibration pattern chip 10R and the green calibration pattern chip 10G is not received by the light receiving portion 300R and the light receiving portion 300G, and it is sometimes difficult to measure the desired color information.

[0411] Here, the "optical path length" can be obtained by the following formula.

[0412] (optical path length of calibration pattern chip that has passed through the light source) x (refractive index of calibration pattern chip) = optical path length

[0413] In addition, the "uniformized optical path length" is only required to be a degree that high-precision measurement can be performed by the observation device using the calibration sliding glass, and for example, it is preferably at least 15% or less, and particularly preferably 10% or less, of the difference in the optical path length in the thickness direction of all the calibration pattern chips that are the same measurement target.

[0414] Figure 23 is a mode diagram showing an example in which the optical path length in the thickness direction of the calibration pattern chip that is the same measurement target is uniformly adjusted in the calibration sliding glass shown in (b). Figure 22 (b) shown, when non-parallel light is irradiated to the calibration sliding glass as the light source h, the non-parallel light that has passed through the red calibration pattern chip 10R and the green calibration pattern chip 10G is not received by the light receiving portion 300R and the light receiving portion 300G, and it is sometimes difficult to measure the desired color information. Figure 22In (b), the IR cutoff filter 80 is configured such that the red calibration pattern chip 10R and the green calibration pattern chip 10G overlap when viewed from above, while the white calibration pattern chip 10W does not have an IR cutoff filter 80 configured. Figure 22 The sliding glass used for calibration shown is not uniform in optical path length at this point. Therefore, in Figure 23 In the calibration sliding glass shown, an adjustment filter 8' with a refractive index similar to the IR cutoff filter 80 is also provided in an overlapping manner when viewed from above within the white calibration pattern chip 10W, uniformly adjusting the optical path length of each calibration pattern chip in the thickness direction. Therefore, for example, when the white calibration pattern chip 10W is used as a reference color and measurements of the red calibration pattern chip 10R and the green calibration pattern chip 10G are performed, accurate calibration can be performed. Furthermore, as a result, measurements with higher accuracy can be performed using an observation device with a camera that incorporates the calibration sliding glass equipped with calibration pattern chips. Additionally, the adjustment filter 8' used here is a component whose material and / or thickness have been adjusted to approximate the refractive index of the IR cutoff filter 80. Therefore, as the adjustment filter 8', a component made of the same material as the basic material of the IR cutoff filter 80 can be used, or the IR cutoff filter 80 can also be used. As specific examples of adjusting the color filter 8' to the former, examples include sodium carbonate glass, quartz glass, and optical glass such as BK7.

[0415] In this invention, as a method for homogenizing the optical path length in the thickness direction of all calibration pattern chips that are for the same measurement object, one method can be listed as making the number of layers of calibration pattern chips that are for the same measurement object consistent. Furthermore, when making the number of layers consistent, it is preferable to select materials whose constituent layers are similar. This is because it allows for more uniform optical path length. In addition to the effect of homogenizing the optical path length, as an effect obtained by making the number of layers consistent, for example, the following effect can be considered: by making the number of components arranged in the optical path consistent, the number of interfaces through which light passes can be consistent, and the refraction of light can be consistent.

[0416] Specifically, such as Figure 24 As shown in (a), when there are red calibration pattern chip 10R and green calibration pattern chip 10G, and a gap exists for white calibration pattern chip 10W, the number of layers for red calibration pattern chip 10R and green calibration pattern chip 10G is 1, while the number of layers for white calibration pattern chip 10W is 0, and the number of layers differs from each other. In this case, for example, as... Figure 24As shown in (b), the white calibration pattern chip 10W is preferably provided with a layer made of a material with a refractive index similar to that of the red calibration pattern chip 10R and the green calibration pattern chip 10G. For example, sodium carbonate glass can be used as a layer made of a material with a refractive index similar to that of the red calibration pattern chip 10R and the green calibration pattern chip 10G.

[0417] In addition, such as Figure 25 As shown in (a), when a red calibration pattern chip 10R, a white calibration pattern chip 10W, and a green calibration pattern chip 10G are provided, and the IR cutoff color filter 80 is configured to overlap with the red calibration pattern chip 10R and the green calibration pattern chip 10G when viewed from above, the number of layers for the red calibration pattern chip 10R and the green calibration pattern chip 10G is 2, while the number of layers for the white calibration pattern chip 10W is 1, and the number of layers differs from each other. In this case, for example, as... Figure 25 As shown in (b), an adjustment filter 8' with a refractive index similar to that of the IR cutoff filter 80 is preferably configured to overlap with the white calibration pattern chip 10W when viewed from above.

[0418] In addition, Figure 24 (a), (b) and Figure 25 The symbols not specified in (a) and (b) can be used with the symbols mentioned above. Figure 15 The same applies, therefore the description here is omitted. Furthermore, in Figure 24 (a), (b) and Figure 25 In (a) and (b), the first and second isolation components are omitted for the sake of simplification, as are the colored and transparent parts of the calibration pattern chip.

[0419] Here, the measurement results were compared when non-parallel light (microspectrophotometer OLYMPUS: OSP-SP200) was used as the light source, with the calibration pattern chips, which are the same measurement object, having uniform and non-uniform optical path lengths in the thickness direction. Specifically, calibration pattern chips 10a and 10b, and an ND filter 90 that overlaps only with the calibration pattern chip 10a when viewed from above, were prepared. Figure 26 (a) shows a sliding glass for calibration, which is equipped with calibration pattern chips 10a and 10b, an ND filter 90 that overlaps with the calibration pattern chip 10a when viewed from above, and an adjustment filter 90' that overlaps with the calibration pattern chip 10b when viewed from above and contains the same material as the ND filter 90. Figure 26(b) is a slide glass for calibration. The above-mentioned pattern chip for calibration uses a soda lime glass, and a filter for which the transmittance is designed to be around 60% is used as the above-mentioned ND filter. Further, the adjustment filter 90' uses a transparent glass containing the same material as the base material of the ND filter 90. In the measurement, as shown in Figure 26 (a), (b), non-parallel light h is irradiated, and light that has respectively transmitted through the pattern chips for calibration 10a and 10b, the ND filter 90, and the adjustment filter 90' is received by the light receiving portions 300a and 300b, and the transmittance is measured. The results of the measurement are shown in Figure 27 (a), the transmittance is around 10%, and correct measurement cannot be performed. On the other hand, in the case of (b), the transmittance is around 60% as designed, and correct measurement can be performed. Figure 26 (a), the transmittance is around 10%, and correct measurement cannot be performed. On the other hand, in the case of (b), the transmittance is around 60% as designed, and correct measurement can be performed. Figure 26 (a), the transmittance is around 10%, and correct measurement cannot be performed. On the other hand, in the case of (b), the transmittance is around 60% as designed, and correct measurement can be performed.

[0420] In the present application, for example, the optical path lengths in the thickness direction of all of the pattern chips for calibration that become the same measurement targets are uniform and Figure 25 (b), or Figure 26 In the case where the IR cut filter 80 or the ND filter 90 shown in (b) is configured so as to overlap with the adjacent pattern chip for calibration in plan view, the IR cut filter 80 or the ND filter 90 can be separately configured in each of the pattern chips for calibration, or can be integrally configured in each of the pattern chips for calibration.

[0421] E. Protective substrate

[0422] The protective substrate in the present application is a member that is configured so as to oppose the pattern chip for calibration and the first spacer, and has at least a transmissive portion in a region that overlaps in plan view with the pattern chip for calibration. Here, the "transmissive portion" refers to a region that transmits at least visible light, and for example, refers to a region of the symbol 20 shown in (b), (c). Figure 13 The shape of the transmissive portion is generally rectangular as shown in (b), (c). Figure 13 The shape of the transmissive portion is generally rectangular as shown in (b), (c).

[0423] The protective substrate in the present application only needs to have at least a transmissive portion in a region that overlaps in plan view with the pattern chip for calibration. Specifically, for example, as shown in (c), it is sufficient that the width w1 of the transmissive portion is smaller than the width w2 of the pattern chip for calibration 10a, and that both ends of the transmissive portion are located more inward than both ends of the pattern chip for calibration. Figure 13 The shape of the transmissive portion is generally rectangular as shown in (b), (c).

[0424] In the present application, the positions and widths of the through portions in the pair of protective substrates are preferably the same. This is because the outline of the calibration pattern chip becomes clear, and a calibration sliding glass of higher quality can be produced. Further, in the case where the positions and widths of the through portions in the pair of protective substrates are different, it is preferable that, from the through portion of the protective substrate provided on the observation side of the calibration sliding glass, the area other than the through portion of the protective substrate provided on the side opposite to the observation side of the calibration sliding glass cannot be observed. That is, in the present application, for example, as shown in FIG. 11, in the case where the widths of the through portions 21 in the pair of protective substrates 20a, 20b are different, the side indicated by an arrow of the one having a narrower width of the through portion 21 is preferably set as the observation side of the calibration sliding glass. Figure 17

[0425] As described above, in the pair of protective substrates, in the case where the widths (opening sizes) of the through portions in the pair of protective substrates are different, the difference is preferably within 0.2 mm, and particularly preferably within 0.1 mm. By the difference in the positions and widths of the through portions in the pair of protective substrates being within the above range, the outline of the calibration pattern chip when the calibration sliding glass is observed from the predetermined surface becomes clear, and a calibration sliding glass of high quality can be produced. Further, the difference in the positions and widths of the through portions in the pair of protective substrates corresponds to, for example, the symbol w3 shown in FIG. 12. Figure 17

[0426] Further, the width of the through portion in the protective substrate can be appropriately adjusted according to the design of the calibration sliding glass, and is not particularly limited.

[0427] The size of the protective substrate can be appropriately selected according to the size of the calibration sliding glass of the present application, and is not particularly limited. As a specific size of the protective substrate, for example, the short side is preferably 26 ± 0.1 mm, and the long side is preferably 76 ± 0.1 mm. Further, as the thickness of the protective substrate, for example, it is preferably within the range of 0.2 mm to 0.4 mm.

[0428] The material used for the protective substrate is, for example, preferably a material that can protect the calibration pattern held by the pair of protective substrates from breakage or dust. As a specific material of the protective substrate, for example, glass, plastic, and the like can be exemplified. In the case where the calibration sliding glass of the present application is used in a microscope with a photographing device, the material of the protective substrate is typically glass. Further, in the case where plastic is used as the material of the protective substrate, from the viewpoint of being able to achieve better microscope measurement, it is preferable to use plastic that does not contain fillers or the like.

[0429] ​​As the method of forming the protective substrate, any method can be used as long as it is a method that can form a desired protective substrate having a transmission portion. For example, a method in which a silver salt emulsion is applied to the surface of a substrate having colorless light transmission, and desilvering treatment is performed on a predetermined region to form a transmission portion, thereby serving as a protective substrate, can be exemplified. In addition, the protective substrate can be composed of one layer or can be composed of multiple layers.

[0430] F. Sealing portion

[0431] In the present application, it is preferable that a sealing portion be provided between the pair of protective substrates along the outer periphery of the protective substrates.

[0432] Figure 18 is a schematic perspective view showing an example of the calibration sliding glass of the present application. As shown in Figure 18 , it is preferable that the calibration sliding glass 200 of the present application have a sealing portion 50 provided between the pair of protective substrates 20a, 20b along the outer periphery of the protective substrates 20a, 20b. By having the sealing portion 50, the side surfaces of the first partition 30 and the second partition 40 can be prevented from being exposed, and the mechanical strength of the calibration sliding glass 200 can be improved. In addition, for example, when a microscope with a photographing device using the calibration sliding glass of the present application is used in a medical site or the like, intrusion of a solvent or the like into the inside of the calibration sliding glass can be prevented. In addition, as for the symbols not explained in Figure 18 , the same as in Figure 13 (a) to (c) is used, and thus the explanation thereof is omitted here.

[0433] As shown in Figure 18 , it is preferable that the sealing portion in the present application be provided between the pair of protective substrates 20a, 20b along the outer periphery of the protective substrates 20a, 20b, thereby having a function of preventing the side surfaces of the first partition 30 and the second partition 40 from being exposed. The sealing portion in the present application can be formed, for example, by flowing a curing agent along the outer periphery of the protective substrates and then curing it. In addition, the sealing portion in the present application can also be formed, for example, by processing a sealing layer having a resin material into a predetermined shape. As the specific curing agent or resin material used for the sealing portion, any material can be used as long as it is a material that can form a desired sealing portion, and there is no particular limitation, and for example, a general curing agent or resin material can be exemplified. In addition, the resin material can have transmittance or can have light blocking properties.

[0434] The thickness of the sealing portion in the present application can be appropriately adjusted depending on the structure of the calibration sliding glass of the present application, and there is no particular limitation, and generally, it corresponds to the distance between the pair of protective substrates, and corresponds to the sum of the thickness of the first partition and the thickness of the second partition. Thus, the description of the specific thickness of the sealing portion is omitted.

[0435] In the present application, as a method of arranging the seal portion along the outer periphery of the protective substrate between a pair of protective substrates, for example, in the case where the seal portion has a resin material, a method of arranging the seal portion by adhering the seal portion to the protective substrate can be exemplified. Here, in adhering the seal portion to the protective substrate, an adhesive is generally used, and in the present application, it is preferable to use a liquid adhesive. This is because, compared to the case where an adhesive sheet or the like is used, an increase in thickness due to the adhesive can be suppressed, and a high adhesive strength can be obtained. In addition, as to the specific material used as the adhesive, the same as the general material can be used, and thus the description thereof is omitted here.

[0436] In the case where a liquid adhesive is used when arranging the seal portion, a groove for arranging the adhesive can also be provided on the surface of the seal portion. In addition, the "surface of the seal portion" here refers to the face that contacts the protective substrate, and thus generally refers to both faces of the seal portion.

[0437] G. Origin mark

[0438] In the present application, it is preferable that an origin mark be provided on the surface of one of the pair of protective substrates. In addition, the "one of the pair of protective substrates" here refers to the protective substrate that generally becomes the observation side of the calibration sliding glass. Furthermore, the "surface of the protective substrate" here refers to the face that generally faces the other of the pair of protective substrates.

[0439] In the present application, in the case where an origin mark is provided on the surface of one of the pair of protective substrates, the origin mark is generally formed at a position that overlaps when viewed from the transmissive portion of the protective substrate, the calibration pattern chip, and the opening portion of the second spacer. This is because the origin mark can be irradiated by the transmitted light that transmits through the calibration sliding glass of the present application.

[0440] Figure 19 (a) is a schematic plan view showing an example of the calibration sliding glass of the present application, Figure 19 (b) is an enlarged view of the region R3 of the calibration sliding glass of the present application shown in (a). As shown in (a) and (b), Figure 19 (a) and (b), it is preferable that the calibration sliding glass 200 of the present application have an origin mark 60 on the surface. In the present application, for example, as shown in (a) and (b), it is preferable to have an origin mark 60x that defines an x-axis or an origin mark 60y that defines a y-axis, and further, as needed, an auxiliary origin mark 60' is preferable. By having an origin mark that defines an x-axis or an origin mark that defines a y-axis, the position information of the calibration pattern or the like can be recognized based on the origin mark. Thus, a calibration sliding glass that copes with an automatic conversion function can be made. Figure 19 Figure 19

[0441] ​​The shape and size of the origin mark in the present application are not particularly limited as long as they are a shape or size that can define an x-axis or a y-axis and can be recognized as an origin mark, and can be appropriately adjusted according to the design of the calibration sliding glass or the like.

[0442] The method of forming the origin mark in the present application is not particularly limited as long as it is a method that can form a desired origin mark, and for example, can be formed by the same method as the through portion in the protective substrate.

[0443] H. Calibration mark

[0444] In the present application, it is preferable to form a calibration mark for preventing positional deviation in the pair of protective substrates, the first spacer, the second spacer, and the like.

[0445] The position at which the calibration mark is formed is not particularly limited as long as it is a region that does not overlap with the calibration pattern and / or the origin mark, and can be appropriately selected according to the configuration of the calibration sliding glass. In addition, the number of calibration marks is also not particularly limited, and can be appropriately adjusted according to the structure of the calibration sliding glass or the like. In the present application, for example, as shown in Figure 18 , calibration marks 70 can be formed on the four sides of each member.

[0446] Figure 20 (a) to (d) are explanatory diagrams for explaining the calibration mark in the present application. In the case where a calibration mark is formed in each member of the calibration sliding glass of the present application, for example, as shown in Figure 20 (a) the protective substrate 20a, Figure 20 (b) the first spacer 30, Figure 20 (c) the second spacer 40, Figure 20 (d) the protective substrate 20b, by forming a calibration mark 70 having a predetermined shape, the position can be aligned based on the calibration mark so that the members overlap when viewed from above. Therefore, when manufacturing the calibration sliding glass of the present application, the positional accuracy when assembling the members can be improved, and a calibration sliding glass of high quality can be produced.

[0447] In the present application, the method of forming a calibration mark in each member is not particularly limited as long as it is a method that can form a desired calibration mark, and can be appropriately selected according to the material used for each member or the like. For example, as a method of forming a calibration mark in a protective substrate, for example, the same forming method as the through portion in the protective substrate can be cited. In addition, as a method of forming a calibration mark in the first spacer and the second spacer, for example, punching processing or etching processing of a metal plate, through processing such as punching processing of a plastic plate, plastic molding, shaping processing such as printing processing, and the like can be cited.

[0448] Example

[0449] The following shows an example to further explain the present application in detail.

[0450] [Example 1]

[0451] A transmission type color calibration chart was produced which consisted of 6 color bars of R, G, B, Ye, Cy, and W, and represented the spectral distribution of Figure 2 and the xy chromaticity diagram of Figure 3 .

[0452] The 5 color bars other than W used a dyed substrate in which a material which is a silver salt emulsion in which a solution of silver nitrate is added to a potassium bromide solution prepared in gelatin and is desilverized from a silver salt photographic plate after drying was used as a substrate, and the substrate was dyed with a dye corresponding to each color. The dye was an acid dye which is generally popular, and the color of each color bar was used. In addition, the dyeing method was the same as the generally popular method, and first a dyeing solution in which an acid dye and an auxiliary agent were mixed was prepared, the substrate was immersed in the dyeing solution, and if the dyeing progressed to a predetermined concentration, the substrate was taken out and washed with water.

[0453] The transmission type color calibration chart was formed by stacking in the order of a transparent protective plate with a light shielding portion, each color bar, and a transparent substrate from the uppermost surface. Each color bar (dyed substrate) was arranged in the order of B, Cy, G, Ye, R, and W on the transparent substrate (glass substrate). In addition, the glass substrate which became the transparent substrate was arranged as the W color bar.

[0454] In addition, the color bars other than W were arranged on the transparent substrate via an IR cut filter. In the B, Cy, and G color bars, an A type (half value T = 50% 620 nm) was used as the IR cut filter, and in the Ye and R color bars, a B type (half value T = 50% 665 nm) was used as the IR cut filter.

[0455] Table 1 shows the peak wavelength of each color bar.

[0456] [Table 1]

[0457]

[0458] [Example 2]

[0459] A transmission type color calibration chart was produced which consisted of 9 color bars of V, NIR, and O in addition to the 6 color bars of Example 1, and represented the spectral distribution of Figure 6 and the xy chromaticity diagram of Figure 7 . The 3 color bars of V, NIR, and O were obtained by the same method as the formation method of the other color bars in Example 1.

[0460] A chart for transmission color calibration was formed in the same manner as in Example 1. Each color bar (dyed substrate) was arranged on a transparent substrate (glass substrate) in the order of V, B, Cy, G, Ye, O, R, NIR, and W. The W color bar was arranged with the glass substrate as the transparent substrate.

[0461] Further, the color bars other than the NIR and W were arranged on the transparent substrate via an IR cut filter. In the V, B, Cy, and G color bars, the A type (half value T = 50% at 620 nm) was used as the IR cut filter, and in the Ye, O, and R color bars, the B type (half value T = 50% at 665 nm) was used as the IR cut filter.

[0462] [Example 3]

[0463] A chart for transmission color calibration was formed in the same manner as in Example 1. Each color bar (dyed substrate) was arranged on a transparent substrate (glass substrate) in the order of V, B, Cy, G, Ye, O, R, NIR, and W. The W color bar was arranged with the glass substrate as the transparent substrate. Figure 9 Figure 10 A chart for transmission color calibration was formed in the same manner as in Example 1. Each color bar (dyed substrate) was arranged on a transparent substrate (glass substrate) in the order of V, B, Cy, G, Ye, O, R, NIR, and W. The W color bar was arranged with the glass substrate as the transparent substrate.

[0464] A chart for transmission color calibration was formed in the same manner as in Example 1. Each color bar (dyed substrate) was arranged on a transparent substrate (glass substrate) in the order of V, B, Cy, G, Ye, O, R, NIR, and W. The W color bar was arranged with the glass substrate as the transparent substrate.

[0465] Further, the color bars other than the NIR and W were arranged on the transparent substrate via an IR cut filter. In the V, B, Cy, and G color bars, the A type (half value T = 50% at 620 nm) was used as the IR cut filter, and in the Ye, O, and R color bars, the B type (half value T = 50% at 665 nm) was used as the IR cut filter.

[0466] The relative wavelengths of the V and NIR color bars and the peak wavelengths of the O and Mg color bars are shown in Table 2.

[0467] [Table 2]

[0468]

[0469] In the xy chromaticity diagram of the chart for transmission color calibration obtained by Examples 1 to 3, Table 3 shows the inclusion rate of the pointer colors included in the color gamut. In addition, the color gamut is a pentagon within which the coordinates of the 5 colors except for W are connected in Figure 3 , and is an octagon within which the coordinates of the 8 colors except for W and Mg are connected in Figure 7 and Figure 10 .

[0470] [Table 3]

[0471] ​

[0472] Explanation of symbols

[0473] 1 transparent substrate

[0474] 2 color bar group

[0475] 3 light-shielding portion

[0476] 4 color bar holding frame

[0477] 12 color bar

[0478] 100 transmission type color calibration chart (color chart)

[0479] 10 calibration pattern

[0480] 10a, 10b calibration pattern chip

[0481] 10c calibration pattern chip (blank)

[0482] 20a, 20b protective base material

[0483] 30 first spacer

[0484] 40 second spacer

[0485] 50 sealing portion

[0486] 60 origin mark

[0487] 70 calibration mark

[0488] 200 calibration sliding glass

Claims

1. A sliding glass for calibration, characterized by, having: a calibration pattern having a plurality of calibration pattern chips; a first spacer disposed around the calibration pattern; and a pair of protective substrates disposed so as to face each other across the calibration pattern and the first spacer, the pair of protective substrates having at least a transmission portion in an area overlapping the calibration pattern in plan view, all of the calibration pattern chips that become the same measurement target among the plurality of calibration pattern chips are uniform in optical path length in the thickness direction, the plurality of calibration pattern chips include an upper calibration pattern chip and a lower calibration pattern chip, between the upper calibration pattern chip and the lower calibration pattern chip, there is a blank area having no color transmittance, between the two transmission portions of the pair of protective substrates in the area where at least one of the calibration pattern chips that become the same measurement target is disposed, an IR cut filter or an ND filter is disposed, between the two transmission portions of the pair of protective substrates in the area where the other calibration pattern chip that becomes the same measurement target is disposed, an adjustment filter is disposed.

2. The calibration sliding glass according to claim 1, wherein the adjustment filter is composed of the same material as the base material of the IR cut filter or the ND filter.

3. The calibration sliding glass according to claim 1 or 2, wherein all of the calibration pattern chips that become the same measurement target among the plurality of calibration pattern chips are the same in the number of layers of components in the thickness direction. ​

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