Specimen section staining device

Abstract

The present invention discloses a specimen slice staining device, wherein the slice is located between two filter screens, and the slice is parallel to the two filter screens, and the area of ​​the slice is smaller than the area of ​​the two filter screens, and the two filter screens are detachably connected, and when the two filter screens are connected, the slice is squeezed between the two filter screens. The present invention discloses a specimen slice staining device, which can prevent the slice from being damaged, and can improve the uniformity of the slice staining, thereby improving the accuracy of the test results, and at the same time, it is not easy to make the slice fall during the staining process, so as to improve the efficiency of the experiment, and the present invention has a simple structure and low cost.

Description

Technical Field

[0001] The invention relates to the technical field of plant anatomy, and more particularly to a specimen slice staining device. Background Art

[0002] In plant dissection experiments, before using an electron microscope to observe and study plant tissue specimens, the plant tissue specimens need to be sliced, stained, cleaned and dried in sequence.

[0003] At present, the conventional method for staining tissue specimen slices of plants is to bond the slices to the slide by glue for staining. First, the staff applies glue (this glue is a conventional tool in this field) on a plane of the slide, and then places the slice on the slide coated with glue by tweezers, so as to bond the slice to the slide by glue; secondly, the slide bonded with the slice is placed in a container containing a dye to stain the slice; finally, the slide bonded with the slice is placed in a container containing distilled water to clean the slice. In the process of staining and cleaning the slice, the slide bonded with the slice needs to be shaken in the corresponding liquid, but because the slice is bonded to the slide by glue, the slice is easy to detach from the slide and fall into the corresponding liquid, which will add trouble to the experimental process, thus resulting in low experimental efficiency. At the same time, since one side of the slice is bonded to the slide, the side of the slice bonded to the slide is difficult to be stained during the staining process, so that the stain can only penetrate the plant tissue cells on the slice through one side of the slice, resulting in uneven staining of the slice, thus affecting the observation results of the plant tissue.

[0004] Moreover, slices are generally obtained by cutting plant tissue specimens with a microtome. Since the slices are very thin and small, they are easily rolled up. However, the slices need to be flat when placed on a glass slide. In order to make the slices flat on the glass slide, the slices are now placed on a position coated with glue on the glass slide with tweezers. During the process, the staff flattens the rolled-up part of the slice with tweezers. However, this can easily damage the slice, thereby damaging the plant tissue cells on the slice, thereby affecting the observation results of the plant tissue.

[0005] Meanwhile, the staining devices on the market that are not easy to damage the slices and can make the slices more uniform are relatively complex, which not only makes the operation complicated but also makes the cost high.

[0006] Therefore, how to provide a specimen section staining device that is not easy to damage the sections, improves the uniformity of section staining, and is not easy to cause the sections to fall during the staining process, and has a simple structure and low cost is a technical problem that technical personnel in this field urgently need to solve. Summary of the invention

[0007] The present invention provides a specimen slice staining device, which can prevent the slices from being damaged and improve the uniformity of the slice staining, thereby improving the accuracy of the test results. At the same time, the slices are not likely to fall off during the staining process, thereby improving the efficiency of the experiment. The present invention has a simple structure and low cost.

[0008] In order to achieve the above object, the present invention adopts the following technical solution:

[0009] A specimen section staining device comprises: two filter screens, a section is located between the two filter screens, and the section is parallel to the two filter screens, and the area of ​​the section is smaller than the area of ​​the two filter screens, and the two filter screens are detachably connected, and when the two filter screens are connected, the section is squeezed between the two filter screens.

[0010] Preferably, it also includes: two pieces of filter paper, the two pieces of filter paper are sandwiched between the two pieces of filter nets, and the slice is located between the two pieces of filter paper, and the slice is parallel to the two pieces of filter paper, and the area of ​​the two pieces of filter paper is larger than the area of ​​the slice.

[0011] Preferably, the area of ​​each piece of filter paper is equal to the area of ​​each piece of filter screen.

[0012] Preferably, the centers of the slice, the two pieces of filter paper and the two pieces of filter screens are collinear.

[0013] Preferably, it further comprises: a clip, wherein the clip is clamped on the two filter nets.

[0014] Preferably, the clamp is close to the slice.

[0015] Preferably, it also includes: a grid frame, which is in the shape of a strip with a cavity, and a plurality of slots are provided on the side walls of the grid frame at intervals along the axial direction of the grid frame, and the plurality of slots are connected to the cavity, and two pieces of the filter screen with the slices can be inserted and clamped in each of the slots.

[0016] Preferably, the plurality of slots are collinear.

[0017] It can be seen from the above technical solutions that, compared with the prior art, the present invention discloses a specimen section staining device, which can achieve the following technical effects:

[0018] The present invention can not only prevent the slices from being damaged, improve the uniformity of the slice dyeing, and prevent the slices from falling off during the dyeing process, but also has a simple structure, convenient operation, and low cost.

[0019] The present invention can improve the experimental efficiency by using the grid support. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0021] Figure 1 This is an exploded view of a specimen section staining device in Example 1 of the present invention;

[0022] Figure 2 This is an exploded view of a specimen section staining device in Example 2 of the present invention;

[0023] Figure 3 This is a schematic diagram of the overall structure of a specimen section staining device of the present invention;

[0024] Figure 4 It is a structural schematic diagram of the grid frame of the present invention.

[0025] Among them, 1-filter net; 2-slice; 3-filter paper; 4-clip; 5-grid rack; 50-slot; 500-cavity. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0028] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0029] Example 1, see Figure 1 A specimen slice staining device comprises: two filter screens 1 and a slice 2; the slice 2 is located between the two filter screens 1, and the slice 2 is parallel to the two filter screens 1, and the area of ​​the slice 2 is smaller than the area of ​​the two filter screens 1, and the two filter screens 1 are detachably connected, and when the two filter screens 1 are connected, the slice 2 is squeezed between the two filter screens 1.

[0030] In the experiment of staining plant specimens, after the plant tissue specimens are cut into slices 2 by a slicer, the staff places the slices 2 on a filter screen 1 by tweezers, and then presses another filter screen 2 on the slices 2. At the same time, since the area of ​​the slices 2 is smaller than the areas of the two filter screens 1, the outer edges of the two filter screens 1 are directly in contact with each other, and then the two filter screens 1 are connected together, so that the slices 2 are squeezed between the two filter screens 1.

[0031] Then, the two filter screens 1 that are clamped with the slice 2 and connected together are placed in the dye (the dye is placed in a container, which is common knowledge in the art and will not be repeated here), and the staff can clamp the two filter screens 1 with tweezers so that the two filter screens 1 that are clamped with the slice 2 and connected together can be shaken in the dye for dyeing. After a period of dyeing time (the dyeing time period is common knowledge in the art and will not be repeated here), the two filter screens 1 that are clamped with the slice 2 and connected together are taken out of the dye by tweezers and placed in distilled water (distilled water is placed in a container, which is common knowledge in the art and will not be repeated here), and the staff can clamp the two filter screens 1 with tweezers so that the two filter screens 1 that are clamped with the slice 2 and connected together can be shaken in the distilled water for cleaning.

[0032] The principle of placing the present invention in other solutions is the same as the above-mentioned principle of placing the present invention in a dye and in distilled water, respectively. How to dye and clean the slices, as well as other subsequent processes are all prior arts and will not be described in detail here.

[0033] After staining, separate the two filters 1, take out the slice 2 onto a slide using tweezers, and then perform subsequent routine operations.

[0034] In the above technical solution of the present invention, after the slice 2 is moved onto a filter screen 1, it is pressed onto the slice 2 by another filter screen 1. Since the surface where the filter screen 1 contacts the slice 2 is a plane, the slice 2 is squeezed by the two filter screens 1, which not only stretches it into a flat state, but also makes the slice 2 less likely to be damaged. At the same time, since both sides of the slice 2 are filter screens 1, during the dyeing process, the dye will pass through both sides of the slice 2 at the same time, and thus can penetrate the slice 2. Therefore, the present invention can improve the uniformity of dyeing the slice 2, thereby improving the accuracy of the test results. Moreover, since the slice 2 is squeezed between the two filter screens 1, the slice 2 is not likely to fall off during the dyeing, washing, etc., thereby improving the efficiency of the experiment.

[0035] The present invention, through the above structure, can not only prevent the slices from being damaged, improve the uniformity of the slice dyeing, and prevent the slices from falling during the dyeing process, but also has a simple structure, convenient operation, and low cost.

[0036] In addition, the filter screen 1 can be a stainless steel filter screen. This structure is a prior art and will not be described in detail here.

[0037] Example 2, see Figure 2 In order to further optimize the above technical solution, it also includes: two pieces of filter paper 3, the two pieces of filter paper 3 are sandwiched between the two filter nets 1, and the slice 2 is located between the two pieces of filter paper 3, and the slice 2 is parallel to the two pieces of filter paper 3, and the area of ​​the two pieces of filter paper 3 is larger than the area of ​​the slice 2.

[0038] The specific operation is different from that of Example 1 in that the first filter paper 3 is first laid on the first filter screen 1, the staff places the slice 2 on the first filter paper 3 using tweezers, and then covers the slice 2 with the second filter paper 3, and then covers the second filter screen 1 on the second filter paper 3, and finally connects the two filter screens 1 together, so that the slice 2 is squeezed between the two filter screens 1 through the two filter papers 3, so that the two sides of the slice 2 contact the two filter papers 3 one by one.

[0039] After staining, separate the two filters 1 and place them on the table as a whole, then remove the upper filter 1 and filter paper 3 in turn, and then use tweezers to take out the slice 2 onto the slide, or hold the filter paper 3 at the bottom to pour the slice 2 onto the slide, and then perform subsequent routine operations.

[0040] In the present invention, filter paper 3 is arranged between each side of the slice 2 and the corresponding filter screen 1, so that each side of the slice 2 is in contact with the corresponding filter screen 1 through the corresponding filter paper 3, so that the slice 2 is not attached to the filter screen 1, so that after dyeing, the slice 2 is easy to take out, and it is not easy to cause damage to the slice 2.

[0041] Furthermore, since the areas of the two filter papers 3 are larger than the area of ​​the slice 2, the slice 2 is less likely to contact the filter screen 1, thereby improving the isolation effect of the filter paper 3 of the present application (the filter paper 3 isolates the slice 2 from the filter screen 1).

[0042] In order to further optimize the above technical solution, the area of ​​each piece of filter paper 3 is equal to the area of ​​each piece of filter screen 1 .

[0043] The area of ​​each piece of filter paper 3 is less than, equal to or greater than the area of ​​each piece of filter mesh 1, which can solve the technical problem of the present application. If the area of ​​each piece of filter paper 3 is equal to the area of ​​each piece of filter mesh 1, there will be no excess filter paper 3 on the periphery of the filter mesh 1, which is not only convenient for dyeing but also saves dye. At the same time, the area of ​​each piece of filter paper 3 is equal to the area of ​​each piece of filter mesh 1, which makes each piece of filter paper 3 more stably sandwiched between two pieces of filter mesh 1 and prevents each piece of filter paper 3 from falling off.

[0044] In order to further optimize the above technical solution, the centers of the slice 2, the two filter papers 3 and the two filter screens 1 are collinear.

[0045] The present application adopts the above technical solution to prevent the slice 2 from falling off easily.

[0046] See also Figure 3 In order to further optimize the above technical solution, it also includes: a clip 4, which is clamped on the two filter screens 1.

[0047] The present application adopts the above-mentioned technical solution. The present invention realizes detachable connection of the two filter screens 1 by clamping the two filter screens 1 with a clip 4, that is, by clamping the two filter screens 1 with the clip 4, the two filter screens 1 can be connected together, and when the clip 4 is removed, the two filter screens 1 can be separated, so that the structure of the present invention is simple, the operation is convenient, and the cost is low.

[0048] The clip 4 may be a dovetail clip.

[0049] In order to further optimize the above technical solution, the clamp 4 is close to the slice 2.

[0050] See also Figure 3The position of the clamp 4 between the two filter screens 1 is such that the clamp 4 is close to the slice 2 but does not contact the slice 2, so that the point of application of the clamping force of the clamp 4 on the two filter screens 1 is close to the center of the slice 2, the filter screen 1 and the filter paper 3, and the distance L between the point of application of the clamping force of the clamp 4 on the two filter screens 1 and the side of the slice 2 close to the clamp 4 is close to 0, thereby not only avoiding the clamp 4 from affecting the staining effect of the slice 2, but also preventing the slice 2 from falling off.

[0051] See also Figures 1 to 4 In order to further optimize the above technical solution, it also includes: a grid frame 5, which is a strip with a cavity 500, and a plurality of slots 50 are spaced apart on the side wall of the grid frame 5 along the axial direction of the grid frame 5, and the plurality of slots 50 are connected to the cavity 500, and two filter screens 1 with slices 2 can be inserted and clamped in each slot 50.

[0052] The size of each slot 50 of the present invention is determined according to actual conditions. For example, when the slice 2 is only sandwiched between two filter screens 1, the thickness of the slot 50 can be equal to the sum of the thickness of the slice 2 and the two filter screens 1; when two filter papers 3 are sandwiched between the two filter screens 1, and the slice 2 is sandwiched between the two filter papers 3, at this time, since the thickness of the filter paper 3 is relatively thin, the thickness of the slot 50 can be equal to the sum of the thickness of the slice 2 and the two filter screens 1, or it can be slightly greater than the sum of the thickness of the slice 2 and the two filter screens 1 (so that the thickness of the slot 50 is equal to the sum of the thickness of the slice 2, the two filter papers 3 and the two filter screens 1); when two filter papers 3 are sandwiched between the two filter screens 1, and the slice 2 is sandwiched between the two filter papers 3 , when a clip 4 is clamped on the two filter nets 1, when only the structure formed by the slice 2, the two filter papers 3 and the two filter nets 1 is inserted into the slot 50, at this time, since the thickness of the filter paper 3 is relatively thin, the thickness of the slot 50 can be equal to the sum of the thicknesses of the slice 2 and the two filter nets 1, or can be slightly larger than the sum of the thicknesses of the slice 2 and the two filter nets 1 (so that the thickness of the slot 50 is equal to the sum of the thicknesses of the slice 2, the two filter papers 3 and the two filter nets 1), and when the structure formed by the slice 2, the two filter papers 3 and the two filter nets 1, and the clip 4 is inserted into the slot 50 at the same time, then the thickness of the slot 50 can be equal to the sum of the thicknesses of the slice 2, the two filter papers 3, the two filter nets 1 and the clip 4.

[0053] The present invention can stain multiple slices 2 at the same time through the grid rack 5, that is, the grid rack 5 with multiple slices 2 installed is placed in the dye, and the grid rack 5 is shaken in the dye with tweezers, so that the multiple slices 2 can be stained at the same time, so the present invention can improve the experimental efficiency.

[0054] In order to further optimize the above technical solution, a plurality of slots 50 are arranged in a common line.

[0055] The multiple slots 50 of the present invention are arranged in a co-linear manner, so that the multiple slices 2 mounted on the same grid frame 5 can be stained more evenly, thereby improving the experimental effect.

[0056] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0057] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A specimen section staining device, characterized in that: include: Two filter nets (1), the slice (2) is located between the two filter nets (1), and the slice (2) is parallel to the two filter nets (1), and the area of ​​the slice (2) is smaller than the area of ​​the two filter nets (1), and the two filter nets (1) are detachably connected, and when the two filter nets (1) are connected, the slice (2) is squeezed between the two filter nets (1); It also comprises: two pieces of filter paper (3), the two pieces of filter paper (3) are sandwiched between the two pieces of filter nets (1), and the slice (2) is located between the two pieces of filter paper (3), and the slice (2) is parallel to the two pieces of filter paper (3), and the areas of the two pieces of filter paper (3) are larger than the area of ​​the slice (2); the slice (2) is squeezed between the two pieces of filter nets (1) by the two pieces of filter paper (3), so that the two sides of the slice (2) contact the two pieces of filter paper (3) one by one; the area of ​​each piece of filter paper (3) is equal to the area of ​​each piece of filter net (1); It also includes: a clip (4), the clip (4) being clamped on the two filter screens (1); The invention also comprises: a grid frame (5), the grid frame (5) being in the shape of a strip having a cavity (500), and a plurality of slots (50) being arranged on the side wall of the grid frame (5) at intervals along the axial direction of the grid frame (5), and the plurality of slots (50) are all connected to the cavity (500), and two pieces of the filter screen (1) having the slices (2) can be inserted and clamped in each of the slots (50).

2. A specimen section staining device according to claim 1, characterized in that: The centers of the slice (2), the two pieces of filter paper (3) and the two pieces of filter nets (1) are collinear.

3. A specimen section staining device according to claim 1, characterized in that: The clamp (4) is close to the slice (2).

4. The specimen section staining device according to claim 1, characterized in that: The plurality of slots (50) are arranged in a common line.

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