A new resistance gene to Agrobacterium tumefaciens infection in rose

Abstract

The present invention relates to rose plants comprising one or more Agrobacterium tumefaciens resistance genes. The present invention also relates to plant parts, cells or reproductive tissues of plants disclosed herein and methods for identifying Agrobacterium tumefaciens resistant rose plants. In particular, the present invention relates to Agrobacterium tumefaciens resistant rose plants, wherein Agrobacterium tumefaciens resistance is provided by one or more genes in linkage group 7 (LG7) located between 56Mb and 58.5Mb of the diploid rose consensus map, in particular one or more genes selected from the group consisting of SEQ ID No. 1 to 5.

Description

[0001] describe

[0002] This invention relates to rose plants containing one or more Agrobacterium tumefaciens resistance genes. The invention also relates to plant parts, cells, or reproductive tissues of the plants disclosed herein, and methods for identifying Agrobacterium tumefaciens-resistant rose plants.

[0003] Crown gall is a bacterial disease caused by Agrobacterium tumefaciens. Infection with Agrobacterium tumefaciens can lead to gall formation, stunted growth, chlorotic leaves, and increased susceptibility to environmental conditions and secondary infections, potentially causing significant economic losses to rose growers worldwide.

[0004] In several other crops, the development of symptoms after inoculation has been shown to be heritable, and based on results obtained using QTL analysis, there is evidence of dominant single-gene inheritance in several crops (such as cabbage (Brassica oleracea), grape (Vitis vinifera), and barley (Hordeum vulgare)), as well as evidence of possible recessive inheritance of resistance in round-leaf cherry (Prunus mahaleb).

[0005] In Arabidopsis thaliana, over 100 mutations leading to resistance to Agrobacterium tumefaciens transformation have been described in genes encoding proteins that may play a role in plant-bacterial interactions, such as cell wall structure and signal transduction. Mutations in genes involved in general defense responses, such as the transcription factor wrky17, have also been found to result in hypersusceptibility of the same crop to Agrobacterium tumefaciens transformation.

[0006] However, in roses, genetic resources (such as genes or genomic regions) that provide resistance to Agrobacterium tumefaciens, or in other words, that prevent gall formation after Agrobacterium tumefaciens inoculation, have not yet been identified. Identifying genes or genomic regions that provide resistance to Agrobacterium tumefaciens would be of great economic value to rose breeders and growers.

[0007] Therefore, in the field of rose breeding, there is a need to provide genomic regions and genes that provide resistance to Agrobacterium tumefaciens in rose plants.

[0008] Among other purposes, one object of the present invention is to meet this need in the field of rose breeding.

[0009] According to the present invention, the above-mentioned needs in the art are met as set forth in the appended claims.

[0010] Specifically, according to the present invention, the above-mentioned requirement is met by providing Agrobacterium tumefaciens-resistant rose plants, wherein Agrobacterium tumefaciens resistance is provided by one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map. The diploid rose homology map is disclosed by Raymond et al. (2018); “The Rosa genome provides new insights into the domestication of modern roses” Nat. Genet. 50:772–778. Furthermore, a rose genome browsing program can be accessed at https: / / lipm-browsers.toulouse.inra.fr / pub / RchiOBHm-V2 / . The resistance of the present invention is characterized in the art as incomplete dominant resistance.

[0011] One or more genes or the entire region between 56 Mb and 58.5 Mb in linkage group 7 (LG7) of the present invention, located on the diploid rose homology map, can be derived from or originate from the rose plant deposited on September 23, 2019, under NCIMB 43507 (NCIMB, Craibstone Estate, Ferguson Building, Aberdeen AB21 9YA, United Kingdom). According to a preferred embodiment, one or more genes or the entire region between 56 Mb and 58.5 Mb in linkage group 7 (LG7) of the present invention, located on the diploid rose homology map, are identical to the corresponding genes or regions in the rose plant deposited under NCIMB 43507.

[0012] According to a preferred embodiment of the invention, one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 6. SEQ ID No. 6 is identified as RchiOBHmChr7g0232931 in the diploid rose homology map (position: stop = 57131496 start = 57128076).

[0013] In *Agrobacterium tumefaciens*-resistant rose plants, the nucleotide at position RoseV2_RchiOBHmChr7_57128943 is mutated from T to A compared to *Agrobacterium tumefaciens*-susceptible plants. The upstream and downstream nucleotides surrounding position RoseV2_RchiOBHmChr7_57128943 (in *Agrobacterium tumefaciens*-resistant rose plants) are represented by SEQ ID No. 11.

[0014] According to another preferred embodiment of the invention, one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 7. SEQ ID No. 7 is identified as RchiOBHmChr7g0232831 in the diploid rose homology map (position: start = 57010720 end = 57017672).

[0015] In *Agrobacterium tumefaciens*-resistant rose plants, the nucleotide at position RoseV2_RchiOBHmChr7_57010803 is mutated from C to G compared to *Agrobacterium tumefaciens*-susceptible plants. The upstream and downstream nucleotides surrounding position RoseV2_RchiOBHmChr7_57010803 (in *Agrobacterium tumefaciens*-resistant rose plants) are represented by SEQ ID No. 12.

[0016] According to another preferred embodiment of the invention, one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 8. SEQ ID No. 8 is identified as RchiOBHmChr7g0232901 in the diploid rose homology map (position: start = 57087871 end = 57092473).

[0017] The absence of the coding sequence (SEQ ID No. 8) is associated with susceptibility to Agrobacterium tumefaciens, while the presence of the coding sequence (SEQ ID No. 8) is associated with resistance to Agrobacterium tumefaciens.

[0018] According to another preferred embodiment of the invention, one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 9. SEQ ID No. 9 is identified as RchiOBHmChr7g0232961 in the diploid rose homology map (position: start = 57174178 end = 57174936).

[0019] In *Agrobacterium tumefaciens*-resistant rose plants, the nucleotide at position RoseV2_RchiOBHmChr7_57175177, located 241 nucleotides upstream of the start codon, is A, compared to *Agrobacterium tumefaciens*-susceptible plants. The upstream and downstream nucleotides surrounding position RoseV2_RchiOBHmChr7_57175177 (in *Agrobacterium tumefaciens*-resistant rose plants) are represented by SEQ ID No. 13.

[0020] According to another preferred embodiment of the invention, one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 10. SEQ ID No. 10 is identified as RchiOBHmChr7g0232471 in the diploid rose homology map (position: start = 56575965 end = 56579420).

[0021] In *Agrobacterium tumefaciens*-resistant rose plants, the nucleotide at position RoseV2_RchiOBHmChr7_56578406 is mutated from C to G compared to *Agrobacterium tumefaciens*-susceptible plants. The upstream and downstream nucleotides surrounding position RoseV2_RchiOBHmChr7_56578406 (in *Agrobacterium tumefaciens*-resistant rose plants) are represented by SEQ ID No. 14.

[0022] In view of the foregoing, the present invention relates to the following most preferred embodiments:

[0023] - Agrobacterium tumefaciens-resistant rose plants, wherein one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 1.

[0024] - Agrobacterium tumefaciens-resistant rose plants, wherein one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 2.

[0025] - Agrobacterium tumefaciens-resistant rose plants, wherein one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 3.

[0026] - Agrobacterium tumefaciens-resistant rose plants, wherein one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 4.

[0027] - Agrobacterium tumefaciens-resistant rose plants, wherein one or more genes in linkage group 7 (LG7) located between 56 Mb and 58.5 Mb in the diploid rose homology map are genes encoding the cDNA sequence of SEQ ID No. 5.

[0028] According to a particularly preferred embodiment, the rose plant of the present invention is a hybrid rose plant.

[0029] According to yet another particularly preferred embodiment, the Agrobacterium tumefaciens-resistant rose plant of the present invention comprises at least two copies of one or more genes of the present invention that provide Agrobacterium tumefaciens resistance.

[0030] According to yet another particularly preferred embodiment, the present invention relates to an Agrobacterium tumefaciens-resistant rose plant comprising a tetraploid genome and at least three copies, preferably four copies, of one or more genes of the present invention providing Agrobacterium tumefaciens resistance.

[0031] According to yet another particularly preferred embodiment, the Agrobacterium tumefaciens-resistant rose plant of the present invention comprises a combination of resistance-providing genes as defined above, such as the following combinations: SEQ ID No. 1 and SEQ ID No. 2 or SEQ ID No. 11 and SEQ ID No. 12; SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 or SEQ ID No. 11, SEQ ID No. 12 and SEQ ID No. 13; SEQ ID No. 1 to 4; SEQ ID No. 1 to 5 or SEQ ID No. 11 to 14.

[0032] The present invention also relates to the plant parts, cells or reproductive tissues of the Agrobacterium tumefaciens-resistant rose plants of the present invention.

[0033] The present invention also relates to a method for identifying Agrobacterium tumefaciens-resistant rose plants, the method comprising the steps of: determining in the genome of the rose plant the presence of one or more genes or copies of their alleles encoding cDNA sequences selected from the group consisting of SEQ ID Nos. 1 to 5, or determining in the genome of the rose plant the presence of one or more sequences selected from the group consisting of SEQ ID Nos. 11 to 14.

[0034] The present invention will be described in further detail in the embodiments presented below. Example

[0035] Materials and Methods

[0036] Population formation and phenotypic screening

[0037] The tetraploid F1 modern rose (Rosa hybrida) population was created by artificial pollination of tetraploid cut roses using rose accession material. This hybridization produced 353 F1 individuals, whose resistance to Agrobacterium tumefaciens was tested. The parents were also tested. The Agrobacterium tumefaciens isolate used originated in the Netherlands and was isolated in 1992. This isolate was propagated on artificial media and grew at a growth rate of 2.6 × 10⁻⁶. 9 The final inoculation density was defined as colony-forming units / ml. One week after inoculation, five rooted cuttings were transplanted into 14 cm pots for each registration material (F1 individuals and parents). A randomized block design was used for inoculation, with four plants per registration material. One plant per registration material was inoculated with water. Plants were inoculated by making incisions in the stems using a scalpel dipped in the inoculum. The scalpel was re-dipping after each incision. Bioassays were performed under long-day conditions, with daytime and nighttime temperatures set at 22°C and 20°C, respectively. Relative humidity was set at 70%. For each plant, nodule presence was scored and size measured at 3, 5, 7, 9, 11, and 14 weeks post-inoculation.

[0038] Phenotypic data analysis

[0039] Tuberculum size and presence were scored across a total of 8572 observations from 1412 plants of 353 unique F1 genotypes. Response variables were analyzed using a mixed model framework with the sommer package in R (Covarrubias-Pazaran 2016). Genotype and block were fitted as random effects, and for tumor size, the observation weeks were included as a fixed effect (covariate). Generalized heritability was then calculated by dividing the genotype variance by the sum of the variance components. The best linear unbiased predictor (BLUP) for each genotype was obtained and used as the corrected phenotype for downstream analyses. The corrected phenotype was centered at 0 (which defines the population mean), and biases were expressed in units of measurement.

[0040] Genetic analysis

[0041] Genotyping: Genotyping was performed on a group consisting of F1 plants, parents, and extensive germplasm registration materials using the WagRhSNP Axiom SNP array. The array contained 68,893 SNPs targeted from each direction by two probes. Initial quality control and dose calling were performed using the R package FitPoly (non-default settings: p.threshold = 0.95, call.threshold = 0.65, peak.threshold = 0.975). Further QC was performed using a custom script in R. Non-dissociative SNPs (SNPs with a frequency >81% for the most common genotype) and SNP markers with more than 10% missing data were removed, resulting in a dataset containing 46,539 markers. A total of 298 F1 individuals were successfully genotyped.

[0042] By using a local blast (set: -evalue 1 -outfmt 6 -max_target_seqs 1 -max_hsps1), the flanking sequences are compared with the reference (from ( https: / / lipm-browsers.toulouse.inra.fr / pub / RchiOBHm- V2 / Download the blast engine and map the SNPs to the rose genome assembly (Raymond et al. 2018). Use a custom script in R to retain only blast hits with an alignment length greater than 50 and a percentage of identical matches greater than 85%.

[0043] Association analysis: Biomarker-trait analysis was performed using the sommer package in R (Covarrubias-Pazaran 2016) within a mixed-model GWAS framework, where SNPs were fitted as fixed effects and the genomic relation matrix (GRM) was fitted as random effects to account for population structure and residual polygenic effects (genetic effects not attributable to the SNPs of interest). Adjusted phenotypes were analyzed by fitting the GRM as random effects, and genomic heritability was similarly calculated.

[0044] Annotation Analysis: Gene predictions from QTL regions were extracted from two files. The first file is the Eugene Annotation v1.1 without duplicates (RchiOBHm-V2-EGN-r1.1.without_TE.gff). The second file is the gene predictions obtained using blast2Go (RchiOBHm-V2-EGN-r1.blast2go.20170310.MAPPING). Both files were obtained from https: / / lipm-browsers.toulouse.inra.fr / pub / RchiOBHm-V2 / (Raymond et al. 2018).

[0045] A gene is considered a resistance gene if the gene prediction contains at least one of the following terms: arabinogalactan, AtAGP17, cellulose synthase-like, CslB-05, cellulose synthase-like, CslA-09, defense, reticulon, BTI1 (AtRTNLB1), BTI2 (AtRTNLB2), BTI3 (AtRTNLB4), Rab8, GTPase, microtubule, kinin, myosin, muscle. Action proteins, cyclic proteins, input proteins, transport proteins, CAK2M, kinases, phosphatases, (PP2C), VIP1, caspase, GALLS, interactions, CAK2M, caspase, histones, histones, pCsn5-1, DNA, ligases, IVa, nucleosomes, assembly, CAF-1, histones, H3, chaperone GA1, histones, deacetylases, H4, H3-11, H2A, Myb, transcription factors, wrky, wrky17 (Gelvin 2010; Lacroix and Citovsky 2013).

[0046] Whole-genome sequencing was performed on a total of 60 extensive germplasm registration materials (including resistance sources). After the reads were mapped to the rose (Rosa chinensis) reference genome (Raymond et al. 2018), the reads were QC and SNPs were determined.

[0047] Validation using SNP

[0048] We mine highly useful SNPs by: 1) selecting resistance genes in QTL regions; 2) identifying SNPs that show unique or nearly unique alleles in the source; and 3) retaining only SNPs for which we can design KASP assays.

[0049] A KASP assay was designed and first tested on a small group of registered materials to determine if they were amplified. Then, amplified KASP assays were performed on two F1 populations sharing the same resistant parent to identify the SNPs most highly associated with Agrobacterium tumefaciens resistance. After fine mapping, the SNPs were used to screen a broad range of germplasm to determine the presence of resistance alleles in the germplasm.

[0050] result

[0051] The gall incidence (percentage of plants with visible galls) increased from 22.5% 3WAI (weeks post-inoculation) to 65.7% 14WAI. Of the 484 gall-free plants in 14WAI, 35 genotypes were asymptomatic in all four replicates (a total of 140 plants), and an additional 54 genotypes were asymptomatic in three replicates. The remaining asymptomatic plants belonged to genotypes that showed greater heterogeneity in gall scores. The mean gall size increased from 5.69 mm 3WAI to 14.66 mm 14WAI (Table 1), with the largest gall size being 62.65 mm. The average gall size (in mm) at 14 WAI (e.g., resistance / susceptibility and normalization used for experiments) is as follows: RS-1075: 48.8, RS-1183: 38.6, RS-1408: 36.28, RS-1418: 0, RS-9052: 15.9.

[0052] Table 1: Progression of tumor incidence during the 14-week trial period.

[0053]

[0054] a Mean and standard error of gall size. Plants without galls were excluded from the calculation.

[0055] When examining the parameter estimates from the hybrid model, gall size increased significantly over time, averaging 0.76 mm per week (Table 2), and based on visual observation, there was no significant difference in gall growth rate between individual plants.

[0056] Table 2: Parameter estimates for fixed effects

[0057]

[0058] For both traits, the block effect explained the very small variance (<1%, Table 3) in both gall size and pre-tumor development weeks. The range of broadly heritable variation was from 0.67 for gall size to 0.41 for pre-tumor development weeks (Table 3).

[0059] Table 3: Variance components of random effects.

[0060]

[0061] a Broadly defined heredity

[0062] The distributions of the corrected phenotypes for the two traits differed considerably. Goiter size showed a bimodal distribution with parents exhibiting significantly different extreme values, while a multimodal distribution was observed for pre-glandular weeks. For goiter size, the pattern with the smallest goiter size contained the largest number of individuals (83% if a cutoff of 5 is used, and 72% if a cutoff of 0 is used).

[0063] The distribution of gall size is compatible with the following conditions: a single gene forms the basis of resistance, the gene is dominant, and the resistant parent contains two copies of the resistance allele, but the penetrance of the resistance allele is incomplete. The average gall size (14WAI) of registered materials with a corrected phenotype of 5 mm or smaller was 2.4 mm, while the average gall size (14WAI) of registered materials with a corrected phenotype greater than 5 mm was 20.8 mm.

[0064] The genomic heritability (represented by narrow-sense heritability) of the corrected phenotypes for goiter size and pre-tumor development weeks were 0.57 and 0.51, respectively. This indicates that genetics explains a portion of the individual-to-individual variation, but other unknown factors (such as environment) also explain a portion of the phenotypic variation. Genomic heritability provides an upper limit to the variance that can be explained by genome-wide significant markers.

[0065] Using a mixture model, the SNPs on the three linkage groups were significant after multiple Bonferroni correction (significance threshold 1.07 * 10⁻⁶). -6 The most significant SNPs on LG7 explained up to 53% of the phenotypic variance, while the most significant SNPs on the other two LGs (LG1 and LG5) explained only 16% of the phenotypic variance (using linear regression).

[0066] Examining the allele dosage at significant SNPs, the co-segregation of SNPs on LG1 and LG5 suggests that these SNPs should map to the same LG. This is confirmed by the fact that only one of the three markers on LG1 and LG5 remained significant using multiple regression. Allele substitution effects indicate that for the most significant SNP AX-86888149 (Table 4), three genotype classes were observed in F1, with plants scoring 2 or 3 having nearly but not identically small gall sizes, and plants scoring 4 having significantly larger gall sizes. This situation is incompatible with both additive and complete dominance, but compatible with incomplete dominance.

[0067] Table 4: Mean and standard error of gall size for each genotype of AX-86888149.

[0068]

[0069] aOnly the number of registry entries where all duplicates were asymptomatic (14WAI) is shown.

[0070] b The number of individual plants containing the specified genotype is shown.

[0071] Five resistance genes were found in the QTL region LG7 (between 56 Mb and 58.5 Mb) (Table 5).

[0072] Table 5: Resistance genes involved in Agrobacterium resistance near the QTL peak on LG7

[0073] sequence list <110> Domo Group <120> A novel resistance gene against Agrobacterium tumefaciens infection in roses <130> P168647PC00 <160> 14 <170> BiSSAP 1.3.6 <210> 1 <211> 2664 <212> DNA <213> Rosa (genus) <400> 1 atggagtctt tgattgtatc cccgctgctg cagctgctct ttagaaggtt gactgacact 60 gttgttgttg gtcaagtgcg aactcgcaga gagttcagga cggagatcga gaagatgcag 120 gagacgttac cgatgattaa ggcggtgata gaagatgcag aggagcagca gaggaaaaat 180 cagagggtga aggcttggct ggtaaagctc aaagacgtcg cagtcgatgc tgataatctg 240 cttgatgagg ttgccaccct agttctgcgc aagcatttga tgaaagagga attttgccgt 300 ttatatgctt ccgaccgata tcgctctcga catccatctt cgtgctccag aatactgtat 360 gattgccaac atcgtgaatt tctttcttca cgtacgaatc tgaacagcaa ggacaggatt 420 480 actcttgaat tgatttctac ttaccgtaag aagtcacgca aactaaagga gcttcgtgca 540 agattggatg atgtagccaa ggagatgtct agtttccact tcaaagaaac tcagtcttat 600 ggacggtcaa gtacaatgga gagacgccaa actggaccct ctgtcaatga ttcaaaggtt 660 tatggcagag aagaggatgt ggataaaatt gtggggatgc tattgtcttc cagatagtggt 720 cctgaagttg cattaattcc cattgttgga cttgcaggga tggggaaaac aacacttgct 780 cagttagtct acaatgatct gagggttaca agccactttc aatcttcaat atgggtgtct 840 gtcaatgata attttaaccc ggcaaggatc ataaatcaaa tgttgagtta tgtcggagaaag 900 ggctgccatg actcgtttca gattggggta ctgcaatctc aattgagaga atcgttactg 960 ggaaaaaggt acttgatagt gctcgatgat gtgtggaatg aggatccaga tgaatgggat 1020 aaagtaatga atccattgaa aggctcccca ggtggaagta aaattatact aaccaccccgg 1080 aataagccag ttgcagccat aactagcaca tttcccccat ttcatttgga accactgaga 1140 aaagaagagt gctggaagtt gttcaagcac cgagcctttg cagatggaac agagggtgat 1200 tttccgagac tactgcagat tggtgagaaa atagtagaca agtgcaaagg tgtcccgttg 1260 gttgcaaata ttcttggaat catgctgcgc tttaaaagag aagaaagcga gtggttacat 1320 gtgcaaagga gtgaactatg gagtattgat gcaggtgaga acagaattct atccatcttc 1380 aggctgagtt acaatcattt gccatcgcat ctgaaagctt gctttgcata ttgttcaata 1440 ttcccaaaga attatgagat tataaggaa atgttgatcc atcaatggct cgcacatggc 1500 ttaattcctc atattgtgga gtcttcaatt aggccagagg acactggcaa tgagtatttt 1560 aacaatttgt tgatgatgtt cttcttccaa gaaataaaaa aatatgatgg cagaggtatg 1620 acagaattta aaatgcatga tcatattaat gatcttgcaa aatctgttgc tggagaggaa 1680 tccttgactc tagaacagga aaatgtgcac tgtagtctct caaagacatg tcatatatca 1740 gttgtttgca gttctagctc tgctttgatc cctgaagcct tgtgtaaagc aaagagattg 1800 cgaaccctta atttcctttc accaagagag gattatgtgg aagccattcc aaccatacaa 1860 gcaactttta aacacctcag aatgctgaat ttcagtggat ttggaattaa gaggcttcac 1920 ccagagattg gtgggctact atccttgcgg tatcttgatc tttcaaatac tctcctagag 1980 acattgccag cgactatctg tgatctatgc aatttgcaga cattaaatct ctcaagttgt 2040 agtgagctca gggagttacc gagtggtact accaagttaa taaatctgag acatcttaac 2100 atagatgatt gtccaagact tgctagcatg cccccatcaa tgagtatgct acgacaactt 2160 caaactttgc cggtatatat catcggccgc aaccctgaaa cttctattgc tcagcttgta 2220 tcaatgacaa atctacgagg gaagttaaaa ctcaaatgtc tggaggaggc taaaagtcca 2280 cgtgacgttg tcatgataac agaatggatg aaaacgaaag aattctattc attggaactg 2340 ttgtggcaaa atgatgatga gtgcaagcta gatcataaca gatctaggca agctcgcagg 2400 caaattgatg ataaaattga ttttattctg gtagattctt taactttatc tccctttgta 2460 aggatgttgt caataaatgg ttattcagga accaagttcc cagatgagat gagttgctct 2520 cagaacttga ccgagctaaa cataataaat tgcagaagat gtgaaagtct tcctccgctc 2580 ggtcaactcc ctgtcctcaa gatcctcaac atccaaggaa tgcataatgt agtctgcatt 2640 gctatccctt atagattttc ctga 2664 <210> 2 <211> 4248 <212> DNA <213> Rosa <400> 2 atggattctc atctgcatca ggtgggacga ctattatccg ctgctcctga tgatgttgcc 60 tatgttggaa tctggggaat ggggggcttg ggtaaaacaa ccattgccag ggctgtttat 120 tacaaaattt ctcatcaatt tgatcaccgt tgctttttgg aaaatgtgag ggaaggcttt 180 agcagttacg gtcaagtaca aatgcaactt caatttctat ctggaatctt taagggaata 240 gtcaacagtt tcgatggggg ttacatgact atgttggaaa ggctaagtcg gataaaaatt 300 ctagttgttc tcgacgatgt atcccgttcc ttggaaattg atgccttact cggaagccca 360 caggaacgtt cacttggttg tggtagtaaa gtcattgtaa caactagaga tgaacaagta 420 ctaggtggat ttaggaaata caagcccaag cattaagtg atcctgacgc tcttgaactc 480 ttcaaccaga atgccttcag aaaaatgcca ccctcagaag agtacgttca tctctcaaga 540 cgtgcaatca aatatgctca cggtctgcct ttagcactta aaacctgggg agctcatctt 600 cgtgacagaa gtccatctgt gtggaaagat gagttggaga aaataaagaa aatcccagac 660 ctagatattc accacgtgca ccatgtgctt agaagaagct ttgatggact agatgagtac 720 caaaagaaca tatttctaga tatcgcatgt tttttcaaag gaatgcacat agagtatgta 780 gaaattattc tgaacagttg tggcttcttt gccagcagtg gttaagcgt tctaattgac 840 agagctcttg taagtatctc aaggtttggt gagctcgaga tacatgattt cctacaggaa 900 attggtaggg atatcgtctg caaagaacct tggaagcata gtagattatg gagttatgaa 960 gatgttcagg ataccttaac acaaaataag gctatggaag ttgaaggcgt aatgattgaa 1020 ttgtctgact caaaagatat acgtgtagat gctcaagctt ttttcgtat gatgaacctg 1080 agattgctca gaatcactta ccccccatat gtgaaccctt taattgagat cagaaaactt 1140 tttgaagggg aattatggcc ggagcggata tacggttacc taccaaattt cgacggtaaa 1200 ttacacttga atggggacct aaagtttctt tctcataagt tgagggttct cgcttggcat 1260 ggttgccccg taaaaacttt gccgtccaac ttttacccaa agtgtcttgt tgaccttgac 1320 atgcgtcata gccacatcga acagctttgg caagaaccca aggctgcgaa agaattgata 1380 agcatcaatt taagttcctg taaaaatctt aaggaaatcc ccctctgcac tgaggcgccg 1440 aagcttcaga aactaattct taactattgt acaagtttag tggaggtttc cccatccatt 1500 tcagctctta caggccttgt tttcttgagt ttatatggtt gcactgaact taagagtctt 1560 ccaagcaaca ttcatatgat gaaatttctc aagacccttg ttctttccgg ctgctcaaat 1620 cttgagatgt ttcccgagat ttcagaagat atggaggcac tatcggagct taggtagat 1680 cgcactgcga ttaagaaact accatcctca attgaacggc ttcggggact taaatccctt 1740 gatctttacg gctgctcaaa tcttgagatg tttcccgaga tttcagaaga tatggggca 1800 ctatcggagc ttatgttaga tggcagtgca attaaggaaa taccatcctc aattgaacgg 1860 cttcggggac ttaaatcatt aagtatgaaa aactgcacaa gccttgtctg tcttcccgac 1920 agtatctgta atttggcaga ccttacatat ctctacctcg aagggtgctc aaaactttgc 1980 aacttgcctg agaatttggg gaatttaaaa tctctgtgtg aacttaaagt agaggatact 2040 ggtataaaac gactcccggc ctgtatcttg catttgaagc ttggaagatt aaagtttcat 2100 tattgcaaac aaatggaagc ccccctttca tcatggccat catcgattga agatcgttgt 2160 actgttgtgg tgcatcttga ttttagttat tgcaatctga aggtgttatc ggatgccatt 2220 gcttattttc gttcattaaa agtattgaat ttatcaagaa ataacaatct gaagagcttg 2280 cctgcagcca tgaatcaact gggttactta gaacggcttc aattggaaga ttgcaagaga 2340 ctgagatcaa taccagagct ttcatcaaga ataagttgca taaatgcgca taattgcaca 2400 gctttggaag ctgtttcaac accacagtct ccctacgata tcggtcgatg cttcatattt 2460 tctaattgca gtcagctgat acagagagat tttttcagag atattgtaga aactcatttc 2520 cctcctcagg gtaattgttc ccgacccttt tatttctcca ttcctggaac tgaaattcca 2580 gagcagttca tccatcaggg tagggggtct tccgtaactg cccagctacc acaaaattgg 2640 tttcacagca acaagttttt ggggttcgct atttgcgcgg ttactaatca gccccagggt 2700 gttgattatt ggaaactgtc tgctcgatgt ttctgtacct tcaaaggaga tcattgcgag 2760 taccgtttca gtttctcttt gttcaatatt tattttgacg gtttctaccg cgactggctt 2820 gtgtcaaatc acatgttagt gggatatgtg ccatggtctg aatttggcat taatggagag 2880 gaagtgaatg aacgccatta cactgaggcc aaatttgaga tagaattatt gcaccgtgaa 2940 acattggagg aagacggggt actattcgac ccttgcatcg aaaggtgtga cccttgcatc 3000 gaaaggtgtg gagttcgatt cgtttttgcc aacaacgagg atgaggaagt tgcccatcaa 3060 gattttgggg aaccaatggt acaaggtgat aattctgaga tcaggagcct tgagggctgc 3120 tcagctgaac gcagtggtag tgacatcaca agtgacacct ctgatgagga ggagcaatac 3180 cttaaattat cggaagtttt tgaaggtgca aacagagaac cgagttacat cagaaatgag 3240 gacaatgaat catctcggaa gaggaaaact ctggaaagtt ggacacaaga agtcagagtt 3300 acatcgggtc tgatgcaagg ggacctggat gatggtttta actggttctg ccataacaga 3360 catttaatcc ctgacaaagg aactacggat ttaagatgcg ggtatgtttg tacacatcga 3420 cccggctgtt gggccagagc taatgtgcga tctttcattg acgacctaac aatccttgaa 3480 attacttaca ttgggaggca cacttgttgg aaaactcttc caatgccaat gttgacacac 3540 gaagtagag ttacaacagg tatggagact gaaggtcctt ctgatggctt taggtgggtg 3600 aagtatgcc aaacagacat accaggagct aaatccccaa gagtctatta tgtatgttct 3660 cctcaaaatg tcggaggctg catggcaata aaagaagtac agcgctccaa tgataaagaa 3720 atccttcaaa ttacttacat aggaaggcac acatgtacac tagcctccac cagcaatgga 3780 ggaagggttg atagcagtac ctccgcggcc aatggtgcat cgagtgttca tgaccaagtt 3840 gtccaatcac caagttcacc aggagccaat ggtcatggag gaatgtaac caaggagcc 3900 gacaatcctg ttaatgacaa caatatgatc gtgttacaag atgcaagctc acaggatatg 3960 gaaccgacac cctcaaatgt tgcaagtgtt tcatctcaac cagtgatctc atcaccatct 4020 ctgactcaag tttaccttca acctcagtct tgttcgcagc ccattgtta aagatcatct 4080 cctaattcca gaacattgt atctcccac acaggtgt tatgagac caagtcagta 4140 gcacagaagg ctaaacccat gcctaaacct gtgacaaaaa ccacacaag agcagagcc 4200 aaaaggac aaggagga ttccgaac aaaatgtgga agccctga 4248 <210> 3 <211> 3777 <212> DNA <213> Pink (Rosa) <400> 3 atgcagcaga cgttgcagcg gattagagcg gcgatagag atgcagagg gcggcagagg 60 aaagtgata gggtgaagga ttgctgata aatctcaag caatcgcagt cgatgctggt 120 aatctgcttg atgaggttgc cacctagtt ctgcgaagc aattgatgaa agaggaatct 180 cacggactac gtgctgccac tagaagac ttatttagac tacgttacca atgcaacatg 240 tccagggtta ttcacacc aggtgctcag accagatatc tcatattca gctttttgaa 300 gcacaagaag agtttgacaa cgacattatt caaggatac tgctcatct aggaatttggt 360 gagctgcaga gtactaaaat actggcagac ttcaagtg cccacttgc cagtggaaag 420 ggagtaagc agatgtatct aacgaggga gtttattatg agctttatct ttcacgtccg 480 area ttggtgtgat aatgagagag attggagagc gtacaaaata tgaggtaagg 540 600 gagctaacg caagatggaa tgatgtagac aaggaatgt ctaggttcca gttcaaaaaa 660 actgagtctt atagacggtc aactaccagg gagagacgag aaactggacc ctttcccaat 720 ggtcaatgg tctatggcag agaagagat gtgaataaga ttgtgcggat gctattgtct 780 tccagtagtg gtcctgaagt tgcagtaatt cccattgttg gaattggagg gatgggggaaa 840 acaacacttg ctcagttagt ctacaatgat ccgagggtta caagccactt tcaatctttg 900 atgtgggtgt ctgtcaatgg taagtttaac ccggcagaga tcataaatca aatgttgagt 960 tatgtcagag agggcagcca tgacctgttt cagattgggt tgctgcaatc tcaattgaga 1020 1080 gatgaatggg ataaagtaat gaatccattg aaaggctccg agggtggaag taaaattata 1140 ttaaccacct ggaatgaggc ggttgcagac ataactagga catttccctc ttttcgtttg 1200 gaaccactaa gaaaagaaga gtgctggaag ttgttcaagc accgagcctt tgcagatgga 1260 acagaggatg atttccgag gctactgcag atcggtgaga aatatagtaga caagtgccaa 1320 ggtgtcccat ttgttgcaaa tatgcttgga atcatgctgc gctttaaaag agaagcaaac 1380 gattggttac atgtgcaaga aagtgaacta tggagtattg atgcaggtga aaacagaatt 1440 ctatccatct acaggctgat ttacaatcat ttgccattgc atctgatagc ttgctttgca 1500 tattgttcaa tattcccaaa gaattatgag attaataagg aaaagttgat ccatcaatgg 1560 ctcgcacatg gcttaattcc ttataattca gggtcttcat ttgagctgga ggacattggc 1620 aatgagtatt ttaaggattt actaatgatg tctttcttcc aagaagtaag aaaacctgat 1680 gacagaggta tggcagaatt taagatgcat gatcttatta atgatcttgc aaaatcagta 1740 gctggtgagg aattcttgac tctgggagag gaatcctcga ctctaggaca ggatattctc 1800 tcaaagacat gtcatgcatc agttgtttgc agttctagct ctattttgac ccctgaagcc 1860 ttgtgtgaag caaagagatt gcaaaccctc aatttcctgt caccaagaga ggattatatg 1920 gaagccattc caaccgtact agcaactttc aaacatctca gaatgctgaa tttcagtgga 1980 tctggaatta agagtctcca ccaagagatt ggtgggctac tgtccttgcg gtatcttgat 2040 ctatcaaata ctctcctaga gacgatgcca gcgactatct gtggtctgtg caatttgcag 2100 accctgaatc tctcaagttg tattgagctc aaggattac caagtggtac taccaagtta 2160 ataaatctga gacatctgaa catagatgat tgtccaagac ttgctggcat gcccccatcc 2220 atgggaattt tacgacgact tcaaactttg ccggtatata tcgtcggccc caactttgaa 2280 actttattttttcagctctc atcaatgaat ctacgaggga agttaaaact caaatgtctg 2340 gaggatgcta aaattccgtc tgggaacaac atgattaaag tatggatgaa aacgagagaa 2400 ttttcttcat tggaactgtt gtggcaaaat gacgggtgca agctagatca taacagatct 2460 aggcaagctg gcaggcaagt tgatggtcaa actgatctta ttctggtgga ttctttgact 2520 gtatcgccct ctataaaaaa gttgtcaata aatggttatt ctggaaccaa gttcccagag 2580 gagatgagtt ggcctcggaa cttgactgag ctaaatataa ttaattgcag aagatgtgaa 2640 agtcttccctc cactcggtca acttcctgtc ctgagatcc tcacatcca aggaatggat 2700 tctgttgtgc gcattgtgt cgaattctct ggtgaaggtg acagaccgtt tagttctctt 2760 aaagagctat ccctcaaaga ctttcctgaa ttaagaactt ggcgtagtat tgattccgga 2820 gaagttatta cttgcctgga aaacttatt atcacaatt gtcctttttt vakaaccatg 2880 ccatggtttc cacatctccg agacttgaag ctgagcaagt gcatgcagct tgacttagta 2940 tggtcagtat caaagcttac cacactctct acttgtta ttgactcctt tccacagttg 3000 agctttctac caaaaaaatt ggtgcaaac aattcacatc tgatatcatt aactgttact 3060 tcctgcccca atattagctc actacctgaa aatctgggaa acctcactgc tctgaaatcg 3120 ctgaaaattg aatggtgtca cgggctagat actttgccaa gtggactaaa gtacctcact 3180 tcactggaga acttggaat agttgattgt cgtggtttaa tctgtttgcc agaggaaggc 3240 height height width height width height 3300 ttgcctatgg ggatgaaaaa cctcacatcc cttgagaacc tcatgcttat gtgtttaaat 3360 ctggttcatc taccagagat ttttcaatac ctcttggcac ttagaagcct gacgattaga 3420 agctgtgaag agcttacaag tctgccggtg ggactgcaac atgtccagaa tttacaattc 3480 ttggaaattc atcgctgccc gaaactgatg gaattgcctg agtgggtgga gcatcttgtt 3540 tcacttcgtt ccttgaaaat cttagactgc cgagaaataa agttcttgcc aaaaggtcta 3600 caatgtcttg gagcgctcca tcacctgtcc ataattgact gtcctgtcct tgagaagcgc 3660 tgcgagatga aaactggtga ggactggcag aagatatctc atattccata taaacatttt 3720 ggatcatcag cagtgcagca caggcaagac attgcatcca ccacacagaa tccttag 3777 <210> 4 <211> 1359 <212> DNA <213> Rosa <400> 4 ggagccacta acaaaaaaag agtgctagaa gttgttcaag caccgagcct ttgcagatgg 60 aacagaggac ggttttccga gccttctgcg catcgttgaa caaatagtag acaagtgcaa 120 aagtgtccca ttggttgca atattcttgg aagcatgctg cgctttaaaa gagagaaag 180 cgagtggtta catgtgcaaa ggagtgaact atggagtatt gatgcagggg agaacagaat 240 tctgtccatc ttaagggtaa gttacaatca tttgccattg catctgaaag cttgctttgc 300 atattgttca gtattcccaa agaattatga gatataag gaaagttga tccatcaata 360 gctcgcacat gcttattc ctcatagtag aactctca gttaggccgg aggacattgg 420 caatgagtat ttcaattt tattaatt gtctttctctc caatgaata gaaatttga 480 tgacagaggt atggcagaat ttaaatgca tgatcatatt atgatcttg caaatctgt 540 tgctggagag gatacttga ctctaggaca ggaaaatgtc cactatggtc tctcaacac 600 atgtcatcag ttgtatgcag tttagctct gctttgatcc ctgaagcctt gtgtgaagca 660 aagagattgc gaaccctcaa tttcctgtcg ccaagagagg attatatgga agccattcca 720 accatactag caacttta acatctcaga atgctgaatt tcagtggatc tggaatttag 780 agtctccacc aggattgg tgggtaata tccttgcggt atctgatct gtcaaatact 840 accctagaga cgatgccagc gactatctgt gatcgctgcc atttgcagac cctgaatctc 900 tcaagttgtc gtgagctcaa ggagttacca agtggtacta ccaagttaat aaatctgaga 960 catcttaaca tagatgattg tccaagactt gctggcatgc ccccatcgat gggaatttta 1020 caacaacttc aaactttgcc agtatatatc atcggccgca attttgaaac ttctattttt 1080 cagattatct caatgaatct acgagggaag ttaaaaatca aatgtctgga ggaggctaaa 1140 attccatttg gaaacaacat gattaaaaga tggatgctaa cgaaagagtt tcagtcattg 1200 gaactgttgt ggcaaaatga tgggggcaag ctagatcata atagatctag gcaagctggc 1260 aggcaagttg atgatagaac tgaattttgt ctggtagatt ctttgactgt atcgcccttt 1320 ataagaatgt tgtcaataaa tggttattca ggaacctag 1359 <210> 5 <211> 2955 <212> DNA <213> Rosa <400> 5 atgccattac ccaaaaacca ccacctccat ctctacctcc tcttcttcct cttcctcctc 60 tccaacgccg ccgcagccga cgagcttcaa atcctactaa aactcaaatc ctccctccag 120 gactcgaact cccaactctt cgccacatgg gactccaccg ccaattcgtt ttgcaaattc 180 accggaatca cctgcaacga cgtcggttcg gttcgagaaa tcgaactttc gaaccaagaag 240 ctgtcggggt ctcttccgct ggactccata tgccaacttc cctcattaga aaagctcgct 300 ttcgggtcca atttcctgca cggtacaatc acggagact tgagaaactg tacgaagctg 360 aaatacctgg atttggggaa aaacttgttc gcgggttcat tccctgacat atcttccttg 420 tcccaactgg agcatcttca tctgaacggc agctggtttt ccgggatttt cccgtggact 480 tccctcacca acatgactgg tctgattcgt ttgagcttgg gtgacaaccc gtttgatccc 540 agcccgtttc caaaggaagt tgaaaatctt aaaaagctcg agtggctcta cttggcaaac 600 tgcagcatcc aaagcaacaat accaagtgaa atcgggaact tggtcgagct tatcaacctg 660 gagttgtccg ataaacaat gaccggagaa attccggtcg agattggaca gctcaccaag 720 ctctggcagc tggagctcta catcaacagg ttcaccggaa tgcttccttt cgggctaaga 780 aacctcacca ttctcgagaa cttcgacgcc tctgagaatt ttctggaagg cgatttgaac 840 gagttgaggt ttctgaccaa cttggtttct ctgcagctgt atgacaacaa tttttccggc 900 gaagtaccgg aagagttcgg cgaattaaac aagcttgtga atctgtcttt gtacggtaac 960 aagctgaccg gtcctctgcc tcagaaattg ggttcttggt cggagatggg cttcatcgac 1020 gtgtcggaga acttcttgac cgggactatt ccgccggaca tgtgcaacaa agggacgatg 1080 aagcagctgc tcatgcttca gaacaaatta actggcgaaa ttccggcaaa ctacgccaag 1140 tgcacgacgt tgacccggtt cagggtcaac aacaactccc tctccggtgt ggttccggca 1200 ggactttggg gattgccgaa tgtggcaatc attgacatca cttcgaatca aattgaaggg 1260 ccgattactt ccgatatcgg aaacgccaag aagcttgcgc agttgtttgt gagttacaat 1320 cggttatctg gtgaattacc ggacgagctt tcgaagtcaa cttcgttggt ttcggttatg 1380 ttgaataaca atcagttttc cggtaagatt ccggcgaaga taggggactt gaagcaattg 1440 ggtactctgc atttagagag caacttgttg tcttcttcga ttcccaagtc attgggaagc 1500 tgtagtttcc tgagtgactt gaacacggcg aacaactcgc tttccggtga aatcccatca 1560 tctttgggct ctttgccgac cttgaattct ctggatttgt ctcacaacca actctccggt 1620 aaaatcccgg aaagtctagc atctctgaga ctaagcatgc ttgatctttc gcaacaagaga 1680 ctgaccggtg ccgtaccgga atctctctcg attgcagcct aaacggcag cctttccggt 1740 aacccgggtc tctgcagcat ggacatcacc tacttcccac ggtgctcgcc gaaaaaggaa 1800 atgtccgacg atgtcaggac actcattatt tgcttctcag tgggtacagc aatattgttt 1860 1920 aaagaagaat cttgggatgt aaagtctttc catgtaataa ccttcagtga ggatgagatt 1980 2040 gtgtcactag ccaatggcaa agaagtcgcc gtgaagcaca tatggaacac tgatccaagc 2100 ggccggaaaa tgttcaagag caccacaccg atgctcggga gacggggcag tggtagttcg 2160 aaatcgaagg aatttgatgc tgaggtgcag acgttgagct aataggca tgtgaatgtg 2220 gtgaagttgt tctgcagcat tactagtgag gactcgagcc ttttggtcta cgagtactta 2280 ccgaacggaa gcttgtggga tcggctgcac acgtgtgaga agatgaagct tgattgggac 2340 gcaaggtatg agattgcagt cggagcagcc aaagggttgg agtatctgca ccatggctgt 2400 gagaggctag tgattcatag agatgtcaag tcgagtaaca ttttgttaga tgagtttttg 2460 aagccccgga tcgcggattt tgggctcgcc aagatggttc agactaatgc aattaaggac 2520 tcgtctcatg ttgttgctgg aacacacggt tacatagctc ctgaatatgg ttacacctac 2580 aaggtgaatg agaagagcga tgtgtatagc tttggtgtag tactaatgga gctagtgacc 2640 gggaaaaagc cgatagatcc atcctttggg gacaacaagg acatagtgaa ctggatatgt 2700 ggcaacctga agagtagaga gagcgtgtta ggtgtggtgg actcgtacgt tcctgaggcc 2760 tatagggaag aggctatcaa ggtattgaga attgcaattc tatgcacagc taggcttcca 2820 gagctaagac cttccatgag aagtgtcgtt caaatgcttg aagaagctca cgagccaatg 2880 aaattgctgg acattgttat cagcaaagat ggttctagta agaaaatgga agtacttaaa 2940 ggaacagaga agtga 2955 <210> 6 <211> 2664 <212> DNA <213> Rosa <400> 6 atggagtctt tgattgtatc cccgctgctg cagctgctct ttagaaggtt gactgacact 60 gttgttgttg gtcaagtgcg aactcgcaga gagttcagga cggagatcga gaagatgcag 120 gagacgttac cgatgattaa ggcggtgata gaagatgcag aggagcagca gaggaaaaat 180 cagagggtga aggcttggct ggtaaagctc aaagacgtcg cagtcgatgc tgataatctg 240 cttgatgagg ttgccaccct agttctgcgc aagcatttga tgaaagagga attttgccgt 300 ttatatgctt ccgaccgata tcgctctcga catccatctt cgtgctccag aatactgtat 360 gattgccaac atcgtgaatt tctttcttca cgtacgaatc tgaacagcaa ggacaggatt 420 ggcatgatgg tgagaaagat tggaaagcgg acaagatatg aggtaaggaa aacttgctta 480 actcttgaat tgatttctac ttaccgtaag aagtcacgca aactaaagga gcttcgtgca 540 agattggatg atgtagccaa ggagatgtct agtttccact tcaaagaaac tcagtcttat 600 ggacggtcaa gtacaatgga gagacgccaa actggaccct ctgtcaatga ttcaaaggtt 660 tatggcagag aagaggatgt ggataaaatt gtggggatgc tattgtcttc cagatagtggt 720 cctgaagttg cattaattcc cattgttgga cttgcaggga tggggaaaac aacacttgct 780 cagttagtct acaatgatct gagggttaca agccactttc aatcttcaat atgggtgtct 840 gtcaatgata attttaaccc ggcaaggatc ataaatcaaa tgttgagtta tgtcggagaaag 900 ggctgccatg actcgtttca gattggggta ctgcaatctc aattgagaga atcgttactg 960 ggaaaaaggt acttgatagt gctcgatgat gtgtggaatg aggatccaga tgaatgggat 1020 aaagtaatga atccattgaa aggctccccaa ggtggagagaa aaattatact aaccaccccgg 1080 1140 aaaagagt gctggaagtt gttcaagcac cgagcctttg cagatggaac agagggtgat 1200 1260 gttgcaaata ttcttggaat catgctgcgc tttaaaagag aagaaagcga gtggttacat 1320 gtgcaaaagga gtgaactatg gagtattgat gcaggtgaga acagaattct atccatcttc 1380 aggctgagtt acaatcattt gccatcgcat ctgaaagctt gctttgcata ttgttcaata 1440 ttcccaaaga attatgagat taataaggaa atgttgatcc atcaatggct cgcacatggc 1500 ttaattcctc atattgtgga gtcttcaatt aggccagagg acactggcaa tgagtatttt 1560 aacaatttgt tgatgatgtt cttcttccaa gaaataaaaa aatatgatgg cagaggtatg 1620 acagaattta aaatgcatga tcatattaat gatcttgcaa aatctgttgc tggagaggaa 1680 tccttgactc tagaacagga aaatgtgcac tgtagtctct caaagacatg tcatatatca 1740 gttgtttgca gttctagctc tgctttgatc cctgaagcct tgtgtaaagc aaagagattg 1800 cgaaccctta atttcctttc accaagagag gattatgtgg aagccattcc aaccatacta 1860 gcaactttta aacacctcag aatgctgaat ttcagtggat ttggaattaa gaggcttcac 1920 ccagagattg gtgggctact atccttgcgg tatcttgatc tttcaaatac tctcctagag 1980 acattgccag cgactatctg tgatctatgc aatttgcaga cattaaatct ctcaagttgt 2040 agtgagctca gggagttacc gagtggtact accaagttaa taaatctgag acatcttaac 2100 atagatgatt gtccaagact tgctagcatg cccccatcaa tgagtatgct acgacaactt 2160 caaactttgc cggtatatat catcggccgc aaccctgaaa cttctattgc tcagcttgta 2220 tcaatgacaa atctacgagg gaagttaaaa ctcaaatgtc tggaggaggc taaaagtcca 2280 cgtgacgttg tcatgataac agaatggatg aaaacgaaag aattctattc attggaactg 2340 ttgtggcaaa atgatgatga gtgcaagcta gatcataaca gatctaggca agctcgcagg 2400 caaattgatg ataaaattga ttttattctg gtagattctt taactttatc tccctttgta 2460 aggatgttgt caataaatgg ttattcagga accaagttcc cagatgagat gagttgctct 2520 cagaacttga ccgagctaaa cataataaat tgcagaagat gtgaaagtct tcctccgctc 2580 ggtcaactcc ctgtcctcaa gatcctcaac atccaaggaa tgcataatgt agtctgcatt 2640 gctatccctt atagattttc ctga 2664 <210> 7 <211> 4248 <212> DNA <213> Rosa <400> 7 atggattctc atctgcatca ggtgggacga ctattatccg ctgctcctga tgatgttgcc 60 tatgttggaa tctggggaat gggcggcttg ggtaaaacaa cattgccag ggctgttat 120 tacaaaattt ctcatcaatt tgatcaccgt tgctttttgg aaaatgtgag ggaaggcttt 180 agcagttacg gtcaagtaca aatgcaactt caatttctat ctggaatctt taagggaata 240 gtcaacagtt tcgatggggg ttacatgact atgttggaaa ggctaagtcg gataaaaatt 300 ctagttgttc tcgacgatgt atcccgttcc ttggaaattg atgccttact cggaagccca 360 caggaacgtt cacttggttg tggtagtaaa gtcattgtaa caactagaga tgaacaagta 420 ctaggtggat ttaggaaata caagcccaag cattaagtg atcctgacgc tcttgaactc 480 ttcaaccaga atgccttcag aaaaatgcca ccctcagaag agtacgttca tctctcaaga 540 cgtgcaatca aatatgctca cggtctgcct ttagcactta aaacctgggg agctcatctt 600 cgtgacagaa gtccatctgt gtggaaagat gagttggaga aaataaagaa aatcccagac 660 ctagatattc accacgtgca ccatgtgctt agaagaagct ttgatggact agatgagtac 720 caaaagaaca tatttctaga tatcgcatgt tttttcaaag gaatgcacat agagtatgta 780 gaaattattc tgaacagttg tggcttcttt gccagcagtg gtttaagcgt tctaattgac 840 agagctcttg taggattctc aaggtttggt gagctcgaga tacatgattt cctacaggaa 900 960 1020 ttgtctgact caaaagat acgtgtagat gctcaagctt tttttcgtat gatgaacctg 1080 agattgctca gaatcactta ccccccatat gtgaaccctt taattgagat cagaaaactt 1140 tttgaagggg aattatggcc ggagcggata tacggttacc taccaaattt cgacggtaaa 1200 ttacacttga atggggacct aaagtttctt tctcataagt tgagggttct cgcttggcat 1260 ggttgccccg taaaaacttt gccgtccaac ttttacccaa agtgtcttgt tgaccttgac 1320 atgcgtcata gccacatcga acagctttgg caagaaccca aggctggaa agaattgata 1380 agcatcaatt taagttcctg taaaaatctt areaaatcc ccctctgcac tgaggcgccg 1440 aagcttcaga aactaattct taactattgt acaagtttag tggaggtttc cccatccatt 1500 tcagctctta caggccttgt tttcttgagt ttatatggtt gcactgaact taagagtctt 1560 ccaagcaaca ttcatatgat gaaatttctc aagacccttg ttctttccgg ctgctcaaat 1620 cttgagatgt ttcccgagat ttcagaagat atggaggcac tatcggagct taggtagat 1680 cgcactgcga ttaagaaact accatcctca attgaacggc ttcggggact taaatccctt 1740 gatctttacg gctgctcaaa tcttgagatg tttcccgaga tttcagaaga tatggggca 1800 ctatcggagc ttatgttaga tggcagtgca attaaggaaa taccatcctc aattgaacgg 1860 cttcggggac ttaaatcatt aagtatgaaa aactgcacaa gccttgtctg tcttccccgac 1920 agtatctgta atttggcaga ccttacatat ctctacctcg aagggtgctc aaaactttgc 1980 aacttgcctg agaatttggg gaatttaaaa tctctgtgtg aacttaaagt agaggatact 2040 ggtataaaac gactcccggc ctgtatcttg catttgaagc ttggaagatt aaagtttcat 2100 tattgcaaac aaatggaagc ccccctttca tcatggccat catcgattga agatcgttgt 2160 actgttgtgg tgcatcttga tttagttat tgcaatctga aggtgttatc ggatgccatt 2220 gcttattttc gttcattaaa agtattgaat ttatcaagaa ataacaatct gaagagcttg 2280 cctgcagcca tgaatcaact gggttactta gaacggcttc aattggaaga ttgcaagaga 2340 ctgagatcaa taccagagct ttcatcaaga ataagttgca taaatgcgca taattgcaca 2400 gctttggaag ctgtttcaac accacagtct ccctacgata tcggtcgatg cttcatattt 2460 tctaattgca gtcagctgat acagagagat tttttcagag atattgtaga aactcatttc 2520 cctcctcagg gtaattgttc ccgacccttt tatttctcca ttcctggaac tgaaattcca 2580 gagcagttca tccatcaggg tagggggtct tccgtaactg cccagctacc acaaaattgg 2640 tttcacagca acaagttttt ggggttcgct atttgcgcgg ttactaatca gccccagggt 2700 gttgattatt ggaaactgtc tgctcgatgt ttctgtacct tcaaaggaga tcattgcgag 2760 taccgtttca gtttctcttt gttcaatatt tattttgacg gtttctaccg cgactggctt 2820 gtgtcaaatc acatgttagt gggatatgtg ccatggtctg aatttggcat taatggagag 2880 gaagtgaatg aacgccatta cactgaggcc aaatttgaga tagaattatt gcaccgtgaa 2940 acattggagg aagacggggt actattcgac ccttgcatcg aaaggtgtga cccttgcatc 3000 gaaaggtgtg gagttcgatt cgtttttgcc aacaacgagg atgaggaagt tgcccatcaa 3060 gattttgggg aaccaatggt acaaggtgat aattctgaga tcaggagcct tgagggctgc 3120 tcagctgaac gcagtggtag tgacatcaca agtgacacct ctgatgagga ggagcaatac 3180 cttaaattat cggaagtttt tgaaggtgca aacagagaac cgagttacat cagaaatgag 3240 gacaatgaat catctcggaa gaggaaaact ctggaaagtt ggacacaaga agtcagagtt 3300 acatcgggtc tgatgcaagg ggacctggat gatggtttta actggttctg ccataacaga 3360 catttaatcc ctgacaaagg aactacggat ttaagatgcg ggtatgtttg tacacatcga 3420 cccggctgtt gggccagagc taatgtgcga tctttcattg acgacctaac aatccttgaa 3480 attacttaca ttgggaggca cacttgttgg aaaactcttc caatgccaat gttgacacac 3540 caagtaagag ttacaacagg tatggagact gaaggtcctt ctgatggctt taggtgggtg 3600 aagtatgccc aaacagacat accaggagct aaatccccaa gagtctatta tgtatgttct 3660 cctcaaaatg tcggaggctg catggcaata aaggaagtac agcgctccaa tgataaagaa 3720 atccttcaaa ttacttacat aggaaggcac acatgtacac tagcctccac cagcaatgga 3780 ggaagggttg atagcagtac ctccgcggcc aatggtgcat cgagtgttca tgaccaagtt 3840 gtccaatcac caagttcacc aggagccaat ggtcatggag gaaatgtaac cagagtagcc 3900 gacaatcctg ttaatgacaa caatatgatc gtgttacaag atgcaagctc acaggatatg 3960 gaaccgacac cctcaaatgt tgcaagtgtt tcatctcaac cagtgatctc atcaccatct 4020 ctgactcaag ttaaccttca acctcagtct tgttcgcagc ccattgttat aagatcatct 4080 cctaattcca gaacaattgt atctcccaac acagggatgt taatgaagac caagtcagta 4140 gcacagaagg ctaaacccat gcctaaacct gtgacaaaaa ccagcacaag agcaagagcc 4200 caacaaggac caaaggagga ttccgacaac aaaatgtgga agccctga 4248 <210> 8 <211> 3777 <212> DNA <213> Rosa <400> 8 atgcagcaga cgttgcagcg gattagagcg gcgatagaag atgcagagga gcggcagagg 60 aaagtgata gggtgaagga ttgctgata aatctcaag caatcgcagt cgatgctggt 120 aatctgcttg atgaggttgc cacctagtt ctgcgaagc aattgatgaa agaggaatct 180 cacggactac gtgctgccac tagaagac ttatttagac tacgttacca atgcaacatg 240 tccagggtta ttcacacc aggtgctcag accagatatc tcatattca gctttttgaa 300 gcacaagaag agtttgacaa cgacattatt caaggatac tgctcatct aggaatttggt 360 gagctgcaga gtactaaaat actggcagac ttcaagtg cccacttgc cagtggaaag 420 ggaagtaagc agatgtatct aacgagggta gttttatg agcttatct ttcacgtccg 480 aaggacagga ttggtgtgat atgagagag attggagagc gtacaaata tgaggtagg 540 aaaacttgct taactcttga gtcgatttct acttaccgta agagtcacg cagactaag 600 gagcttaacg caatggaa tgatgtagac aaggaaatgt ctaggttcca gttcaaaaaa 660 actgagtctt atagacggtc aactaccagg gagagacgag aactggacc ctttcccaat 720 gagtcaatgg tctatggcag agagaggat gtgaataaga tgtgcggat gctattgtct 780 tccagtagtg gtcctgaagt tgcagtaatt cccattgttg gaattggagg gatggggaaa 840 acaacacttg ctcagttagt ctacaatgat ccgagggtta caagccactt tcaatctttg 900 atgtgggtgt ctgtcaatgg taagtttaac ccggcagaga tcataaatca aatgttgagt 960 tatgtcagag agggcagcca tgacctgttt cagattgggt tgctgcaatc tcaattgaga 1020 gagtccttac tgggaagaag atacttaata gtgcttgatg atgtgtggaa tgtggatgaa 1080 gatgaatggg ataaagtaat gaatccattg aaaggctccg agggtggaag taaaattata 1140 ttaaccacct ggaatgaggc ggttgcagac ataactagga catttccctc ttttcgtttg 1200 gaaccactaa gaaaagaaga gtgctggaag ttgttcaagc accgagcctt tgcagatgga 1260 acagaggatg attttccgag gctactgcag atcggtgaga aaatagtaga caagtgccaa 1320 ggtgtcccat ttgttgcaaa tatgcttgga atcatgctgc gctttaaaag agaagcaaac 1380 gattggttac atgtgcaaga aagtgaacta tggagtattg atgcaggtga aaacagaatt 1440 ctatccatct acaggctgat ttacaatcat ttgccattgc atctgatagc ttgctttgca 1500 tattgttcaa tattcccaaa gaattatgag attaataagg aaaagttgat ccatcaatgg 1560 ctcgcacatg gcttaattcc ttataattca gggtcttcat ttgagctgga ggacattggc 1620 aatgagtatt ttaaggattt actaatgatg tctttcttcc aagaagtaag aaaacctgat 1680 gacagaggta tggcagaatt taagatgcat gatcttatta atgatcttgc aaaatcagta 1740 gctggtgagg aattcttgac tctgggagag gaatcctcga ctctaggaca ggatattctc 1800 tcaaagacat gtcatgcatc agttgtttgc agttctagct ctattttgac ccctgaagcc 1860 ttgtgtgaag caaagagatt gcaaaccctc aatttcctgt caccaagaga ggattatatg 1920 gaagccattc caaccgtact agcaactttc aaacatctca gaatgctgaa tttcagtgga 1980 tctggaatta agagtctcca ccaagagatt ggtgggctac tgtccttgcg gtatcttgat 2040 ctatcaaata ctctcctaga gacgatgcca gcgactatct gtggtctgtg caatttgcag 2100 accctgaatc tctcaagttg tattgagctc aaggagttac caagtggtac taccaagtta 2160 ataaatctga gacatctgaa catagatgat tgtccaagac ttgctggcat gcccccatcc 2220 atgggaattt tacgacgact tcaaactttg ccggtatata tcgtcggccc caactttgaa 2280 acttctattt ttcagctctc atcaatgaat ctacgaggga agttaaaact caaatgtctg 2340 gaggatgcta aaattccgtc tgggaacaac atgattaaag tatggatgaa aacgagagaa 2400 ttttcttcat tggaactgtt gtggcaaaat gacgggtgca agctagatca taacagatct 2460 aggcaagctg gcaggcaagt tgatggtcaa actgatctta ttctggtgga ttctttgact 2520 gtatcgccct ctataaaaaa gttgtcaata aatggttatt ctggaaccaa gttcccagag 2580 gagatgagtt ggcctcggaa cttgactgag ctaaatataa ttaattgcag aagatgtgaa 2640 agtcttcctc cactcggtca acttcctgtc ctgaagatcc tcaacatcca aggaatggat 2700 tctgttgtgc gcattggtgt cgaattctct ggtgaaggtg acagaccgtt tagttctctt 2760 aaagagctat ccctcaaaga ctttcctgaa ttaagaactt ggcgtagtat tgattccgga 2820 gaagtattta cttgcctgga aaaacttatt atcacaaatt gtcctttttt gacaaccatg 2880 ccatggtttc cacatctccg agacttgaag ctgagcaagt gcatgcagct tgacttagta 2940 tggtcagtat caaagcttac cacactctct actcttgtta ttgactcctt tccacagttg 3000 agctttctac caaaaaaatt ggtgcaaaac aattcacatc tgatatcatt aactgttact 3060 tcctgcccca atattagctc actacctgaa aatctgggaa acctcactgc tctgaaatcg 3120 ctgaaaattg aatggtgtca cgggctagat actttgccaa gtggactaaa gtacctcact 3180 tcactggaga acttggaaat agttgattgt cgtggtttaa tctgtttgcc agaggaaggc 3240 atggaaggct tgtgctcact tcggtcattt tcaattgaga actgtttgaa cttaacctct 3300 ttgcctatgg ggatgaaaaa cctcacatcc cttgagaacc tcatgcttat gtgtttaaat 3360 ctggttcatc taccagagat ttttcaatac ctcttggcac ttagaagcct gacgattaga 3420 agctgtgaag agcttacaag tctgccggtg ggactgcaac atgtccagaa tttacaattc 3480 ttggaaattc atcgctgccc gaaactgatg gaattgcctg agtgggtgga gcatcttgtt 3540 tcacttcgtt ccttgaaaat cttagactgc cgagaaataa agttcttgcc aaaaggtcta 3600 caatgtcttg gagcgctcca tcacctgtcc ataattgact gtcctgtcct tgagaagcgc 3660 tgcgagatga aactggtga ggactggcag aagatatctc atattccata taaacatttt 3720 ggatcatcag cagtgcagca caggcaagc attgcatcca ccacacagaa tccttag 3777 <210> 9 <211> 1359 <212> DNA <213> Pink (Rosa) <400> 9 ggagccacta aaaaaaag agtgctagaa gttgttcaag caccgagcct tgcagatgg 60 aacagaggac gttttccga gccttctgcg catcgttgaa caatagtag acagtgcaa 120 aagtgtccca ttggttgca atattcttgg aagcatgctg cgctttaaaa gagagaaag 180 cgagtggtta catgtgcaaa ggagtgaact atggagtatt gatgcagggg agaacagaat 240 tctgtccatc ttaagggtaa gttacaatca tttgccattg catctgaaag cttgctttgc 300 atattgttca gtattcccaa agaattatga gattaatag gaaagttga tccatcaatg 360 gctcgcacat gcttattc ctcatagtag aactctca gttaggccgg aggacattgg 420 caatgagtat ttcaattt tattaatt gtctttctctc caatgaata gaaatttga 480 tgacagaggt atggcagaat ttaaatgca tgatcatatt atgatcttg caaatctgt 540 tgctggagag gatacttga ctctaggaca ggaaaatgtc cactatggtc tctcaacac 600 atgtcatcag ttgtatgcag tttagctct gctttgatcc ctgaagcctt gtgtgaagca 660 aagagattgc gaaccctcaa tttcctgtcg ccaagagagg attatatgga agccattcca 720 accatactag caacttta acatctcaga atgctgaatt tcagtggatc tggaatttag 780 agtctccacc aggattgg tgggtaata tccttgcggt atctgatct gtcaaatact 840 accctagaga cgatgccagc gactatctgt gatcgctgcc atttgcagac cctgaatctc 900 tcaagttgtc gtgagctcaa ggagttacca agtggtacta ccaagttaat aaatctgaga 960 catcttaaca tagattg tccagactt gctggcatgc cccatcgat gggaattta 1020 caacaacttc aactttgcc agtatatatc atcggccgca attttgaac ttctatttt 1080 cagattatct caatgaatct acgaggag ttaaaaatca atgtctgga ggaggctaaa 1140 attccattg gaacaacat gattaaaga tggatgcta cgaagagtt tcagtcattg 1200 gaactgttgt ggcaaatga tggggcaag ctagatcata atagatctag gcaagctggc 1260 aggcaagttg atgatagaac tgaattttgt ctggtagatt ctttgactgt atcgcccttt 1320 ataagaatgt tgtcaataaa tggttattca ggaacctag 1359 <210> 10 <211> 2955 <212> DNA <213> Rosa <400> 10 atgccattac ccaaaaacca ccacctccat ctctacctcc tcttcttcct cttcctcctc 60 tccaacgccg ccgcagccga cgagcttcaa atcctactaa aactcaaatc ctccctccag 120 gactcgaact cccaactctt cgccacatgg gactccaccg ccaattcgtt ttgcaaattc 180 accggaatca cctgcaacga cgtcggttcg gttcgagaaa tcgaactttc gaaccagaag 240 ctgtcggggt ctcttccgct ggactccata tgccaacttc cctcattaga aaagctcgct 300 ttcgggtcca atttcctgca cggtacaatc acggaggact tgagaaactg tacgaagctg 360 aaatacctgg atttggggaa aaacttgttc gcgggttcat tccctgacat atcttccttg 420 tcccaactgg agcatcttca tctgaacggc agctggtttt ccgggatttt cccgtggact 480 tccctcacca acatgactgg tctgattcgt ttgagcttgg gtgacaaccc gtttgatccc 540 agcccgtttc caaaggaagt tgaaaatctt aaaaagctcg agtggctcta cttggcaaac 600 tgcagcatcc aaagcaacaat accaagtgaa atcgggaact tggtcgagct tatcaacctg 660 gagttgtccg ataaacaat gaccggagaa attccggtcg agattggaca gctcaccaag 720 ctctggcagc tggagctcta catcaacagg ttcaccggaa tgcttccttt cgggctaaga 780 aacctcacca ttctcgagaa cttcgacgcc tctgagaatt ttctggaagg cgatttgaac 840 gagttgaggt ttctgaccaa cttggtttct ctgcagctgt atgaacaa tttttccggc 900 gaagtaccgg aagagttcgg cgaattaaag aagcttgtga atctgtcttt gtacggtaac 960 aagctgaccg gtcctctgcc tcagaaattg ggttcttggt cggagatggg cttcatcgac 1020 gtgtcggaga acttcttgac cgggactatt ccgccggaca tgtgcaacaa agggacgatg 1080 aagcagctgc tcatgcttca gaacaaatta actggcgaaa ttccggcaaa ctacgccaag 1140 tgcacgacgt tgacccggtt cagggtcaac aacaactccc tctccggtgt ggttccggca 1200 ggactttggg gattgccgaa tgtggcaatc attgacatca cttcgaatca aattgaaggg 1260 ccgattactt ccgatatcgg aaacgccaag aagcttgcgc agttgtttgt gagttacaat 1320 cggttatctg gtgaattacc ggacgagctt tcgaagtcaa cttcgttggt ttcggttatg 1380 ttgaataaca atcagttttc cggtaagatt ccgggggaaga taggggactt gaagcaattg 1440 ggtactctgc atttagagag caacttgttg tcttcttgga ttcccaagtc attgggaagc 1500 tgtagtttcc tgagtgactt gaacacggcg aacaactcgc tttccggtga aatcccatca 1560 tctttgggct ctttgccgac cttgaattct ctggatttgt ctcacaacca actctccggt 1620 aaaatcccgg aaagtctagc atctctgaga ctaagcatgc ttgatctttc gcaacaagaga 1680 ctgaccggtg ccgtaccgga atctctctcg attgcagcct aaacggcag cctttccggt 1740 aacccgggtc tctgcagcat ggacatcacc tacttcccac ggtgctcgcc gaaaaaggaa 1800 atgtccgacg atgtcaggac actcattatt tgcttctcag tgggtacagc aatattgttt 1860 1920 aaagaagaat cttgggatgt aaagtctttc catgtaataa ccttcagtga ggatgagatt 1980 cttgattcca ttactcaaga gaatctaatt ggaaaaggag gttctggaaa tgtctacaaa 2040 gtgtcactag ccaatggcaa agaagtcgcc gtgaagcaca tatggaacac tgatccaagc 2100 ggccggaaaa tgttcaagag caccacaccg atgctcggga gacggggcag tggtagttcg 2160 aaatcgaagg aatttgatgc tgaggtgcag acgttgagct caataaggca tgtgaatgtg 2220 gtgaagttgt tctgcagcat tactagtgag gactcgagcc ttttggtcta cgagtactta 2280 ccgaacggaa gcttgtggga tcggctgcac acgtgtgaga agatgaagct tgattgggac 2340 gcaaggtatg agattgcagt cggagcagcc aaagggttgg agtatctgca ccatggctgt 2400 gagaggctag tgattcatag agatgtcaag tcgagtaaca ttttgttaga tgagtttttg 2460 aagccccgga tcgcggattt tgggctcgcc aagatggttc agactaatgc aattaaggac 2520 tcgtctcatg ttgttgctgg aacacacggt tacatagctc ctgaatatgg ttacacctac 2580 aaggtgaatg agaagagcga tgtgtatagc tttggtgtag tactaatgga gctagtgacc 2640 gggaaaaagc cgatagatcc atcctttggg gacaacaagg acatagtgaa ctggatatgt 2700 ggcaacctga agagtagaga gagcgtgtta ggtgtggtgg actcgtacgt tcctgaggcc 2760 tatagggaag aggctatcaa ggtattgaga attgcaattc tatgcacagc taggcttcca 2820 gagctaagac cttccatgag aagtgtcgtt caaatgcttg aagaagctca cgagccaatg 2880 aaattgctgg acattgttat cagcaaagat ggttctagta agaaaatgga agtacttaaa 2940 ggaacagaga agtga 2955 <210> 11 <211> 103 <212> DNA <213> Rosa <400> 11 ttcctttcac caagagagga ttatgtggaa gccattccaa ccatacaagc aacttttaaa 60 cacctcagaa tgctgaattt cagtggattt ggaattaaga ggc 103 <210> 12 <211> 101 <212> DNA <213> Rosa <400> 12 gattctcatc tgcatcaggt gggacgacta ttatccgctg ctcctgatga tgttgcctat 60 gttggaatct ggggaatggg gggcttgggt aaaacaacca t 101 <210> 13 <211> 107 <212> DNA <213> Rosa <400> 13 attcccaaag aattatgaga ttaataagga aaagttgatc catcaatagc tcgcacatgg 60 cttaattcct catagtagaa actcttcagt taggccggag gacattg 107 <210> 14 <211> 60 <212> DNA <213> Rosa <400> 14 gtcagcttgt taccgtacaa agacagattc acaagcttgt ttaattcgcc gaactcttcc 60

Claims

1. A method for identifying rose plants resistant to Agrobacterium tumefaciens, the method comprising the following steps: The presence of a gene or an allelic copy thereof encoding each of the cDNA sequences of SEQ ID No. 1 to 5, or a sequence selected from the group consisting of SEQ ID No. 11 to 14, is determined in the genome of the rose plant, wherein the Agrobacterium tumefaciens resistance is provided by a region between 56 Mb and 58.5 Mb in linkage group 7 (LG7) of the diploid rose consensus map of the rose plant deposited as NCIMB43507, and the diploid rose consensus map is disclosed by Raymond et al. (2018); "The Rosagenome provides new insights into the domestication of modern roses" Nat. Genet. 50: 772-778, wherein the rose plant comprises a tetraploid genome and at least 3 copies of one or more genes providing Agrobacterium tumefaciens resistance.

2. The method of claim 1, wherein the rose plant comprises four copies of one or more genes that provide resistance to Agrobacterium tumefaciens.

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