Device for virus screening based on bowl-shaped centrifugal microfluidic chip

Abstract

The present invention relates to a device for virus screening based on a bowl-shaped centrifugal microfluidic chip, wherein the device comprises: a clamping disk interlocked with a base disk and a cover disk; a conical chip layer matched with the clamping disk and configured as a hollow cone with a microporous wall structure; a bowl-shaped chip arranged in a three-dimensional intersecting shape formed by the conical chip layer and the clamping disk; and a motor shaft inserted into the center of the clamping disk, the motor shaft being used to centrifuge and mix the bowl-shaped chip. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention does not require professional operators and is free of aerosol contamination. It takes only 3 minutes to input a trace sample, freeze-dried powder, reagents, and a color developer, and then achieves fully automatic operation and visual rapid detection of multiple targets in the sample through centrifugal mixing and positive pressure drive. This can significantly improve detection accuracy and screening speed, and is widely used in airport customs, community homes, and other places.

Description

Technical Field

[0001] The present invention relates to the fields of in vitro diagnosis and microfluidics, in particular to the field of biological detection technology, and specifically refers to a device for virus screening based on a bowl-shaped centrifugal microfluidics chip. Background Art

[0002] Currently, nasopharyngeal swab virus testing involves multiple steps, is time-consuming, and requires manual labor from specialized lab personnel. This can lead to high false negative rates, aerosol contamination and leakage, and errors during operation. Promoting nucleic acid testing in developing and underdeveloped regions globally presents challenges, including overcoming contamination, reducing costs, and simplifying procedures by eliminating complex instruments.

[0003] Existing microfluidic chips for nucleic acid detection offer a solution by integrating multiple reaction steps within a few square centimeters of microfluidic chip. These chips offer advantages such as controlled fluid flow, low consumption, and rapid analysis. As an emerging scientific technology, they hold great promise for development. They can miniaturize detection instruments, simplify sample pretreatment, improve the efficiency and precision of experimental procedures, and reduce testing costs. Currently, the main detection technologies are based on PCR and isothermal amplification methods, but these still rely on nasopharyngeal swabs, which are prone to flaws and have high false-negative rates. Furthermore, most fluorescence-based assays require specialized equipment for result interpretation, and negative sample interpretation takes a long time due to background fluorescence. Most chips only detect a single pathogen, which cannot meet throughput requirements. Furthermore, most microfluidic technologies require additional control equipment to maintain a stable pressure differential within the channel. These challenges pose challenges to the efficient, accurate, simple, and rapid performance of point-of-care (POCT) testing.

[0004] Existing patent CN 113652341 A discloses a "visually detectable" virus detector. While simple, convenient, and highly practical, it still has some drawbacks and inconveniences, such as the need for a manual syringe and laboratory microinjection pump, heating in a water bath or metal bath (at 65°C for 30 minutes), a one-minute cooling time to room temperature (heating affects the stability of viral nucleic acids, especially RNA, which can affect test results), and the need for a matching centrifuge to mix the solution. The multiple manual operations and time-consuming nature of the process make it impractical for large-scale operation.

[0005] There are also many difficulties and shortcomings in existing virus screening:

[0006] The disconnect between R&D and production technologies has resulted in a large number of microfluidic chip experimental technologies but few large-scale production and applications;

[0007] The disconnect between R&D technology and market application has resulted in many patents for virus test kits, but few that are practical and practical for personal use.

[0008] The disconnect between sample collection and virus extraction results in a high amplification of viruses in throat swabs upon heating, but very little in sputum samples with high viral loads;

[0009] The disconnect between virus extraction and nucleic acid testing has resulted in a large number of virus testing equipment and instruments, but few that are quick, convenient, and labor-saving.

[0010] Faced with the continuous mutation of the new coronavirus, it is urgent to provide frequent, low-cost, rapid and large-scale testing, new detection methods and screening devices with high sensitivity and speed, and the development of simpler and easier-to-promote screening tools. Summary of the Invention

[0011] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a device for virus screening based on a bowl-shaped centrifugal microfluidic chip for performing multi-target detection on a single sample.

[0012] In order to achieve the above objectives, the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention is as follows:

[0013] The device for virus screening based on a bowl-shaped centrifugal microfluidic chip has the following main features:

[0014] A clamping plate provided by engaging the base plate with the cover plate;

[0015] A conical chip layer matching the clamping disk and configured as a hollow cone with a microporous wall structure;

[0016] The conical chip layer and the clamping disk are engaged with each other to form a bowl-shaped chip arranged in a three-dimensional intersecting shape; and a motor shaft is inserted at the center of the clamping disk, and the motor shaft is used to achieve centrifugal mixing processing for the bowl-shaped chip.

[0017] Preferably, the chassis is a flat disc, and a concave opening is provided at the upper end of the chassis, and a central cylinder is sequentially provided inside the chassis for plugging in a straight shaft hole of the motor, and a positioning pin hole is provided at the edge of the straight shaft hole;

[0018] The upper circumferential edge of the chassis is provided with a chassis slot with a bayonet, and the bottom of the chassis is provided with an inwardly concave bottom, and the positioning pin hole and the chassis slot are used to position and engage the cover plate of the clamping plate; and

[0019] The liquid inlet channel of the clamping plate is formed by a combination of a liquid inlet socket, a circular ring reagent kit, an arc reaction groove, a circular tube groove and a groove liquid guide tube.

[0020] Preferably, the box body of the ring test kit is made of plastic, and its annular outer surface is configured with the upper end protruding outward and the lower end tilted inward, and is sealed by a film to prevent the splashing of the reagent;

[0021] The bottom of the circular reagent box is set as a structure with a high center and inclined in the circumferential direction. The circular reagent box is set according to the angle to have N independent arc segments, and each arc segment is divided into a fan-shaped grid with M independent sectors. Each of the fan-shaped grids is pre-buried with one or more powders or reagents, as well as a channel for inputting gas. One end of the gas channel is connected to a micro air pump, and the other end is connected to the circular tube groove and the groove through hole of the groove liquid guide tube.

[0022] Preferably, the arc reaction tank is evenly distributed with several arc cavities in proportion to accommodate reagents and reaction liquids, and each of the arc cavities is pre-embedded with at least one or more virus screening primers for detecting pathogens;

[0023] The circular tube grooves are evenly divided and arranged on the periphery of each section of the circular arc cavity in the same proportion as the circular arc reaction grooves, and the bottom of each section of the circular arc cavity is provided with the groove liquid guide tube; a chisel ring slot is also provided below the groove liquid guide tube.

[0024] Preferably, the circular reagent kit is also configured as two box bodies distributed inside and outside, wherein the inner layer is configured as a small circular reagent kit with a reduced single structure, and the periphery of the small circular reagent kit is provided with a circular liquid storage tank for increasing the amount of reagent added, and primers and probes are also evenly arranged on the periphery of the circular liquid storage tank, and each of the primers and probes is embedded in the circular arc cavity at the corresponding position.

[0025] Preferably, the cover plate is configured as a disc with an axial hole, and the axial hole of the cover plate is provided with an external hexagonal reverse thread nut and a positioning pin provided on the edge of the axial hole;

[0026] A circular hole ring, a liquid inlet socket, a cover plate stop and a cover plate buckle are sequentially arranged outward along the axis hole. The external hexagonal reverse thread nut, positioning pin, cover plate stop and cover plate buckle work together to accurately fit the cover plate on the chassis of the clamping plate; the circular hole ring is used to insert multiple nozzles to respectively input several reagents and input positive pressure gas from the air pump, and the liquid inlet socket is used to be inserted into the liquid inlet funnel to input sample liquid.

[0027] Preferably, the liquid inlet funnel is configured to have an elliptical open shape at the upper part, a funnel shape in the middle part, and a conical through tube at the lower outlet end, which is used to collect oral sputum samples, and the open part of the liquid inlet funnel is provided with an elliptical reagent pack plug of the same size that can be inserted into an extruded plastic reagent pack, a puncture component is provided at the inner edge of the open part of the liquid inlet funnel, and a fiber filter layer is padded in the middle position of the liquid inlet funnel; the two sides of the cone tube at the lower part of the liquid inlet funnel are provided with fascia strips corresponding to the size of the liquid inlet socket and fixedly arranged on the upper part of the cone tube, and the lower part of the fascia strip is a movable strip, which is used to lock and prevent the left and right sides of the liquid inlet funnel from retreating; the reagent pack plug is provided with virus lysis solution, and the puncture component is used to insert and puncture the extruded plastic reagent pack, thereby infusing the virus lysis solution to flush the viscous sputum blocked by the fiber filter layer.

[0028] Preferably, the conical chip layer is provided with a thermoplastic resin material having the same size as the chip base layer, a layer of PET coil is adhered to the outside of the chip base layer as the chip cover layer of the conical chip layer, and a layer of PE coil is adhered to the outside of the chip cover layer as the chip protection layer of the conical chip layer;

[0029] The conical chip layer is also provided with a single-sided chip layer or a double-sided chip layer; the single-sided chip layer is provided with at least four or more screening channels only on the outer surface of the chip base layer; the double-sided chip layer is provided with four or more screening channels on the inner and outer sides of the chip base layer respectively.

[0030] Preferably, the chip cover layer is manufactured by slitting, pressing and curling to have a plurality of large hole ends and small hole ends of preset sizes, each of the large hole ends and small hole ends is respectively arranged at the upper closed circular end and the lower closed circular end of the conical chip layer, and each of the large hole ends and small hole ends is set to a closed state;

[0031] At least four liquid inlet pipes coated with a hot-melt quick-drying adhesive layer are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end; each section of the conical chip layer is also provided with a group of cuts coated with hot-melt quick-drying adhesive along the height direction of the conical chip layer, and each section of the conical chip layer is curled after the cuts are butt-jointed and bonded to form the conical chip layer of each section;

[0032] The small hole end of the conical chip layer is inserted into the inverted wedge ring slot, and the groove liquid guide tube is inserted into the corresponding liquid inlet tube. The end surface of the small hole end of the conical chip layer is coated with hot melt adhesive, and the hot melt adhesive is used to bond the inverted wedge ring slot set on the bottom of the chassis to achieve matching assembly of the bowl-shaped chip.

[0033] Preferably, the conical chip layer is provided with a preset number of screening channels arranged perpendicularly to the conical chip layer or in a parabolic shape along the rotation direction of the conical chip layer, and each of the screening channels is respectively provided with a liquid inlet pipe, at least one diversion channel, an anti-backflow buckle, a pre-assembly port, an upper blocking channel, a lower blocking channel, a square color developing trough and a waste liquid trough, the inlet diameters of the upper blocking channel and the lower blocking channel are both set to be larger than the diameter of the square color developing trough, and the upper blocking channel and the lower blocking channel are connected to the square color developing trough, the waste liquid trough is provided on both sides of the square color developing trough, and absorbent paper is further provided between the square color developing trough and the waste liquid trough, an exhaust groove is provided above the waste liquid trough, and the waste liquid trough is connected to the exhaust groove through an anti-overflow pipe, and an exhaust hole is also provided on the exhaust groove, and the exhaust hole is used to discharge the gas present in the pipeline of the bowl-shaped chip during the input, vibration, centrifugation and positive pressure processes of the liquid sample;

[0034] In addition, a polymer water-absorbing material is pre-embedded in the waste liquid tank, and the upper blocking channel and the lower blocking channel are filled with microballs at the pre-assembly port position. The microballs are used to prevent the reagents from being blocked, and anti-fall buckles are provided at the bottom of the upper blocking channel and the lower blocking channel. The anti-fall buckles are used to prevent the microballs from falling.

[0035] The automated device for virus screening based on a bowl-shaped centrifugal microfluidic chip has the following main features: the automated device comprises the above-mentioned bowl-shaped chip, clamping plate, conical chip layer, bottom plate and cover plate; the bowl-shaped chip is injected with liquid from the top of the bowl-shaped chip using a multi-head peristaltic pump; and the automated device is also provided with a liquid inlet funnel, an aerosol sampler, a medium-temperature sterilization box and a screening window baffle.

[0036] The desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip has the following main features: the device comprises the bowl-shaped chip, a clamping disk, a conical chip layer, a bottom plate and a cover plate, wherein the bowl-shaped chip is extracted from the bottom box by an intelligent vacuum pump and a water pump, and liquid is injected from the top of the bowl-shaped chip; the bowl-shaped chip is composed of a cylindrical outer sleeve, an inner sleeve and a disc-shaped bottom box stacked together; a color sensor is provided on the inner wall of the outer sleeve, and an arc-shaped screen is attached to the arc of the outer wall of the outer sleeve; a preset number of arc-shaped nozzles and an outer sleeve handle are provided on the top of the outer sleeve; a micro motor is provided at the center of the top of the inner sleeve, and a micro motor shaft is provided in the vertically upward protruding direction of the micro motor; a positioning turntable is provided above the micro motor shaft, the positioning turntable includes a rotating disk, a fixed disk provided at the bottom of the rotating disk, and positioning steel balls installed on the fixed disk; the bowl-shaped chip is installed on the rotating disk.

[0037] Preferably, the bottom box includes a bottom cover and a base, and the bottom cover is provided with a large plug-in buckle and a small plug-in buckle, the large plug-in buckle is used to plug and fix the outer sleeve, and the small plug-in buckle is used to plug and fix the inner sleeve; the center position of the bottom cover is provided with an intelligent vacuum water pump, a micro water pump A, a micro water pump B and a control board; the bottom cover is provided on the base, and the base is also provided with a foot, and the base is divided into storage area A, storage area B and storage area C for storing liquid reagents; the intelligent vacuum water pump, micro water pump A and micro water pump B are connected to the storage area A, storage area B and storage area C through a straw, and are connected to each of the arc-shaped nozzles by a plastic tube, and the control board is electrically connected to the intelligent vacuum water pump, micro water pump A, micro water pump B, color sensor, arc-shaped screen and micro motor.

[0038] The wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip has the following main features: the device comprises the above-mentioned bowl-shaped chip, clamping disk, conical chip layer, bottom plate and cover disk, wherein the bowl-shaped chip is divided into a preset number of intervals in a ring test kit, pre-infused with screening reagents, and is set by manually pressing the reagent channel and blowing air through the mouth in a positive pressure manner, and the wearable device operates the switch button to select the function switch, display screen and corresponding display and electrical board, and the screening is achieved by pressing the wearable device onto a vertical cylindrical wearable base for vertical operation.

[0039] The device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention integrates multiple reactions of conventional analytical detection methods onto a bowl-shaped centrifugal microfluidic chip, providing a non-invasive, efficient, and convenient method for airport customs duty around the clock, as well as for direct sputum collection and testing at home. It can be widely used to detect a variety of respiratory viruses, significantly improving the accuracy and speed of test results, as well as the screening rate of asymptomatic or early-stage patients, and avoiding the risk of cross-infection. Furthermore, the device is easy to operate and carry, simple to manufacture, efficient, economical, and durable, making it very suitable for large-scale promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a schematic front view of a bowl-shaped chip according to a first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0041] Figure 2 This is a schematic top view of the chip of the first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0042] Figure 3 This is a schematic diagram of the main chassis of the first embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0043] Figure 4 This is a schematic top view of the chassis of the first embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0044] Figure 5 This is a schematic front view of a reagent kit according to a first embodiment of the present invention for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip.

[0045] Figure 6 This is a schematic top view of a test kit according to a first embodiment of the present invention, which is a device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip.

[0046] Figure 7 This is a schematic front view of the cover plate of the first embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0047] Figure 8 This is a schematic top view of the cover plate of the first embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0048] Figure 9 This is a schematic front view of a clamping disk of the first embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0049] Figure 10This is a schematic diagram of the chip layer of the first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0050] Figure 11 This is a schematic top view of the chip layer of the first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0051] Figure 12 This is a schematic diagram of chip layer bonding in the first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0052] Figure 13 This is a schematic diagram of the liquid inlet funnel of the first embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0053] Figure 14 This is a schematic front view of a clamping disk of a second embodiment of the device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0054] Figure 15 This is a schematic diagram of the main view of the chip of the second embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0055] Figure 16 This is a schematic top view of the chip of the second embodiment of the device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0056] Figure 17 Schematic diagram of an embodiment of an automated device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0057] Figure 18 This is a schematic diagram of a window of an embodiment of the automated device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0058] Figure 19 Schematic diagram of a desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0059] Figure 20 Schematic diagram of the outer sleeve of the desktop device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0060] Figure 21 Schematic diagram of the inner sleeve of the desktop device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0061] Figure 22 Schematic diagram of the rotating disk assembly of the desktop device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0062] Figure 23 This is a schematic diagram of the rotating disk of the desktop device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0063] Figure 24 This is a schematic diagram of the bottom cover of the desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0064] Figure 25 This is a schematic diagram of the base of a desktop device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0065] Figure 26 Schematic diagram of a wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0066] Figure 27 Schematic diagram of chip installation of a wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip of the present invention.

[0067] Figure 28 This is a schematic diagram of the chip structure of a wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

[0068] Figure 29 This is a schematic diagram of the main view of the chip of the wearable device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0069] Figure 30 This is a schematic top view of the chip of the wearable device for virus screening based on the bowl-shaped centrifugal microfluidic chip of the present invention.

[0070] Reference numerals

[0071] 1. Bowl-shaped chip; 2. Clamping plate; 3. Conical chip layer; 4. Bottom plate; 5. Cover plate; 6. Liquid inlet channel; 7. Screening channel; 8. Concave opening; 9. Central cylinder; 10. Straight-through shaft hole; 11. Dowel pin hole; 12. Ring reagent kit; 13. Arc reaction tank; 14. Circular tube groove; 15. Groove guide tube; 16. Bottom plate slot; 17. Bottom plate concave bottom; 18. Screening device; 19. Reagent kit slope; 20. Reagent kit bottom; 21. Ring cavity; 22. Sector grid; 23. Arc cavity; 24. Virus primer; 25. Circular tube cavity; 26. Inverted ring slot; 27. Hexagonal nut; 28. Dowel pin; 29. ​​Circular hole ring; 30. Liquid inlet port; 31. Cover plate stopper; 32. Cover plate buckle; 34. Chip base layer; 35. Chip cover layer; 36. Chip protective layer; 37 Liquid inlet tube; 38 Diversion channel; 39 Anti-backflow clip; 40 Pre-assembly port; 41 Upper blocking channel; 42 Lower blocking channel; 43 Microsphere; 44 Anti-drop clip; 45 Square color development tank; 46 Waste liquid tank; 47 Absorbent paper; 48 Anti-overflow pipe; 49 Exhaust groove; 50 QR code; 51 Exhaust hole; 52 Large hole end; 53 Small hole end; 54 Hot melt adhesive; 55 Chip interface; 56 Liquid inlet funnel; 57 Needle puncture component; 58 Honeycomb sponge; 59 Fascia strip; 60 Movable strip; 61 Reagent pack stopper; 62 Small ring reagent kit; 63 Ring liquid reservoir; 64 Primers and probes; 65 Aerosol sampler; 66 Negative pressure exhaust fan; 67 UV sterilization lamp; 68 Medium temperature sterilization box; 69 Screening window baffle; 70 Disposable mask; 71 Fingerprint controller; 72 Vibration detection armrest; 73 Antibacterial color-coated board; 74 Automatic height-increasing cushion; 75 Control cabinet; 76 Automatic conveyor platform; 77 Negative pressure exhaust fan; 78 Circular conveyor line; 79 Chip fixing block; 80 Micro air pump; 81 Multi-head peristaltic pump; 82 Annular nozzle; 83 Mask bag funnel; 85 Desktop device; 86 Bottom box; 87 Outer sleeve; 88 Color sensor; 89 Arc screen; 90 Arc nozzle; 91 Outer sleeve handle; 92 Inner sleeve; 93 Micro motor; 94 Micro motor shaft; 95 Positioning turntable; 95-1 Rotating disk; 95-2 Fixed disk; 95-3 Positioning steel ball; 96 Bottom cover; 97 Large plug-in buckle; 98 Small plug-in buckle; 99 Intelligent vacuum water pump; 100 Micro pump A; 101 Micro pump B; 102 Control board; 103 Base; 104 Foot; 105 Storage area A; 106 Storage area B; 107 Storage area C; 108 Wearable device; 109 Switch button; 110 Display and board; 111 Wearable base DETAILED DESCRIPTION

[0072] In order to more clearly describe the technical content of the present invention, further description is given below in conjunction with specific embodiments.

[0073] Before describing in detail embodiments according to the present invention, it should be noted that, hereinafter, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, whereby a process, method, article, or apparatus comprising a list of elements includes not only those elements, but also other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0074] See also Figure 1 As shown, the device for virus screening based on a bowl-shaped centrifugal microfluidic chip comprises:

[0075] A clamping plate 2 is provided by engaging a base plate 4 with a cover plate 5;

[0076] A conical chip layer 3 matching the clamping disc 2 and configured as a hollow cone with a microporous wall structure;

[0077] The conical chip layer 3 and the clamping disk 2 are engaged with each other to form a bowl-shaped chip 1 arranged in a three-dimensional intersecting shape; and a motor shaft is inserted at the center of the clamping disk 2, and the motor shaft is used to achieve centrifugal mixing treatment for the bowl-shaped chip 1.

[0078] As a preferred embodiment of the present invention, the chassis 4 is a flat-bottomed disc, and a concave opening 8 is provided at the upper end of the chassis 4. A central cylinder 9 is sequentially provided inside the chassis 4, a straight-through shaft hole 10 for plugging in the motor, and a positioning pin hole 11 provided on the edge of the straight-through shaft hole 10;

[0079] The upper circumferential edge of the chassis 4 is provided with a chassis slot 16 with a bayonet, and the bottom of the chassis 4 is provided with a concave bottom. The positioning pin hole 11 and the chassis slot 16 are used to locate and engage the cover plate 5 of the clamping plate 2; and

[0080] The liquid inlet channel 6 of the clamping plate 2 is formed by the liquid inlet socket 30, the annular reagent kit 12, the arc reaction tank 13, the circular tube groove 14 and the groove liquid guide tube 15.

[0081] As a preferred embodiment of the present invention, the box body of the ring test kit 12 is made of plastic, and its annular outer surface is configured with the upper end protruding outward and the lower end tilted inward, and is sealed by a film to prevent the splashing of the reagent;

[0082] The bottom of the ring test kit 12 is set to a structure with a high center and inclined in the circumferential direction. The ring test kit 12 is set according to the angle to have N independent arc segments, and each arc segment is divided into a fan-shaped grille 22 with M independent sectors. Each of the fan-shaped grilles 22 is pre-buried with one or more powders or reagents, as well as a channel for inputting gas. One end of the gas channel is connected to a micro air pump, and the other end is connected to the circular tube groove 14 and the groove through hole of the groove liquid guide tube 15.

[0083] As a preferred embodiment of the present invention, the arc reaction tank 13 is evenly distributed with a plurality of arc cavities 23 to accommodate reagents and reaction liquids, and each of the arc cavities 23 is pre-embedded with at least one or more virus screening primers 24 for detecting pathogens.

[0084] The circular tube grooves 14 are evenly divided and arranged on the periphery of each section of the circular arc cavity 23 in the same proportion as the circular arc reaction grooves 13, and the bottom of each section of the circular arc cavity 23 is provided with the groove liquid guide tube 15; a chisel ring slot 26 is also provided below the groove liquid guide tube 15.

[0085] As a preferred embodiment of the present invention, the circular reagent kit 12 is also configured as two box bodies distributed inside and outside, wherein the inner layer is configured as a small circular reagent kit 62 with a reduced single structure, and the periphery of the small circular reagent kit 62 is provided with a circular liquid storage tank 63 for increasing the amount of reagent added, and the periphery of the circular liquid storage tank 63 is also evenly provided with primers and probes 64, and each of the primers and probes 64 is embedded in the arc cavity 23 at the corresponding position.

[0086] As a preferred embodiment of the present invention, the cover plate 5 is configured as a circular disc with an axial hole, and the axial hole of the cover plate 5 is provided with an external hexagonal reverse thread nut 27 and a positioning pin 28 provided on the edge of the axial hole;

[0087] Along the axis hole, a circular hole ring 29, a liquid inlet socket 30, a cover plate stop 31 and a cover plate buckle 32 are arranged in sequence outward. The external hexagonal reverse thread nut 27, the positioning pin 28, the cover plate stop 31 and the cover plate buckle 32 work together to accurately fit the cover plate 5 on the chassis 4 of the clamping plate 2; the circular hole ring 29 is used to insert multiple nozzles to respectively input several reagents and input positive pressure gas from the air pump 33, and the liquid inlet socket 30 is used to insert the liquid inlet funnel 56 to input sample liquid.

[0088] As a preferred embodiment of the present invention, the liquid inlet funnel 56 is configured to have an oval opening at the top, a funnel shape in the middle, and a conical through tube at the lower outlet end, for collecting oral sputum samples, and the open end of the liquid inlet funnel 56 is provided with an oval reagent pack plug 61 of the same size that can be plugged into an extruded plastic reagent pack, a needle-piercing member 57 is provided at the inner edge of the open end of the liquid inlet funnel 56, and a fiber filter layer 58 is provided in the middle position of the liquid inlet funnel 56; The two sides of the cone tube at the bottom of the liquid funnel 56 are provided with fascia strips 59 corresponding to the size of the liquid inlet socket 30 and fixedly arranged on the upper part of the cone tube. The lower part of the fascia strips 59 is a movable strip, which is used to lock and prevent the left and right sides of the liquid inlet funnel 56 from retreating; the reagent pack plug 61 is provided with virus lysis liquid, and the puncture member 57 is used to insert and puncture the extruded plastic reagent pack, thereby infusing the virus lysis liquid to flush the viscous sputum blocked by the fiber filter layer 58.

[0089] As a preferred embodiment of the present invention, the conical chip layer 3 is provided with a thermoplastic resin material having the same size as the chip base layer 34, a layer of PET coil is adhered to the outside of the chip base layer 34 as the chip cover layer 35 of the conical chip layer 3, and a layer of PE coil is adhered to the outside of the chip cover layer 35 as the chip protection layer 36 of the conical chip layer 3;

[0090] The conical chip layer 3 is also provided with a single-sided chip layer or a double-sided chip layer; the single-sided chip layer is provided with at least four or more screening channels 7 only on the outer surface of the chip base layer 34; the double-sided chip layer is provided with four or more screening channels 7 on the inner and outer side surfaces of the chip base layer 34 respectively.

[0091] As a preferred embodiment of the present invention, the chip cover layer 35 is manufactured by slitting, pressing and curling to have a plurality of large hole ends 52 and small hole ends 53 of preset sizes, each of which is respectively arranged at the upper closed circular end and the lower closed circular end of the conical chip layer 3, and each of which is set to a closed state;

[0092] At least four liquid inlet pipes coated with a hot-melt quick-drying adhesive layer are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end 53; each section of the conical chip layer 3 is also provided with a group of cuts coated with hot-melt quick-drying adhesive along the height direction of the conical chip layer 3, and each section of the conical chip layer 3 is curled after the cuts are butt-jointed and bonded to form each section of the conical chip layer 3;

[0093] The small hole end 53 of the conical chip layer 3 is inserted into the inverted ring slot 26, and the groove liquid guide tube 15 is inserted into the corresponding liquid inlet tube 37. The end surface of the small hole end 53 of the conical chip layer 3 is coated with hot melt adhesive 54, and the hot melt adhesive 54 is used to bond the inverted ring slot 26 set on the bottom of the chassis 4 to achieve matching assembly of the bowl-shaped chip 1.

[0094] As a preferred embodiment of the present invention, the conical chip layer 3 is provided with a preset number of screening channels 7 arranged vertically along the conical chip layer 3 or parabolically along the rotation direction of the conical chip layer 3, and each of the screening channels 7 is respectively provided with a liquid inlet pipe 37, at least one or more diversion channels 38, an anti-backflow buckle 39, a pre-assembly port 40, an upper blocking channel 41, a lower blocking channel 42, a square color development tank 45 and a waste liquid tank 46. The inlet diameters of the upper blocking channel 41 and the lower blocking channel 42 are both set to be larger than the diameter of the square color development tank 45, and the upper blocking channel 41 and the lower blocking channel 42 are respectively provided with a liquid inlet pipe 37, at least one diversion channel 38, an anti-backflow buckle 39, a pre-assembly port 40, an upper blocking channel 41, a lower blocking channel 42, a square color development tank 45 and a waste liquid tank 46. The plug channel 41 and the lower blocking channel 42 are connected to the square color developing tank 45. The waste liquid tank 46 is provided on both sides of the square color developing tank 45, and absorbent paper 47 is provided between the square color developing tank 45 and the waste liquid tank 46. An exhaust groove 49 is provided above the waste liquid tank 46, and the waste liquid tank 46 is connected to the exhaust groove 49 through an anti-overflow pipe 48. The exhaust groove 49 is also provided with an exhaust hole 51. The exhaust hole 51 is used to discharge the gas in the pipeline of the bowl-shaped chip 1 during the input, vibration, centrifugation and positive pressure processes of the liquid sample;

[0095] In addition, a polymer water-absorbing material is pre-embedded in the waste liquid tank 46, and the upper blocking channel 41 and the lower blocking channel 42 are filled with microspheres 43 at the pre-assembly port position. The microspheres 43 are used to prevent the reagent from being blocked, and anti-fall buckles 44 are provided at the bottom of the upper blocking channel 41 and the lower blocking channel 42. The anti-fall buckles 44 are used to prevent the microspheres 43 from falling.

[0096] This automated device for virus screening based on a bowl-shaped centrifugal microfluidic chip, wherein the automated device comprises the above-mentioned bowl-shaped chip 1, clamping plate 2, conical chip layer 3, bottom plate 4 and cover plate 5, wherein the bowl-shaped chip 1 is injected with liquid from the top of the bowl-shaped chip 1 using a multi-head peristaltic pump, and the automated device is also provided with a liquid inlet funnel 56, an aerosol sampler 65, a medium-temperature sterilization box 68 and a screening window baffle 69.

[0097] The desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip, wherein the device comprises the bowl-shaped chip 1, the clamping plate 2, the conical chip layer 3, the bottom plate 4 and the cover plate 5 described above, wherein the bowl-shaped chip 1 is extracted from the bottom box by an intelligent vacuum pump and a water pump, and liquid is injected from the top of the bowl-shaped chip 1, and the bowl-shaped chip 1 is composed of a cylindrical outer sleeve 87, an inner sleeve 92 and a disc-shaped bottom box 86 stacked together, a color sensor 88 is provided on the inner wall of the outer sleeve 87, and an arc-shaped screen 89 is attached to the arc of the outer wall of the outer sleeve 87, and the A preset number of arc-shaped nozzles 90 and an outer sleeve handle 91 are provided at the top of the outer sleeve 87; a micro motor 93 is provided at the top center of the inner sleeve 92, and a micro motor shaft 94 is provided in the vertical upward protruding direction of the micro motor 93, and a positioning turntable 95 is provided above the micro motor shaft 94, and the positioning turntable 95 includes a rotating disk 95-1, a fixed disk 95-2 provided at the bottom of the rotating disk 95-1, and a positioning steel ball 95-3 installed on the fixed disk 95-2, and the bowl-shaped chip 1 is installed on the rotating disk 95-1.

[0098] As a preferred embodiment of the present invention, the bottom box 86 includes a bottom cover 96 and a base 103. The bottom cover 96 is provided with a large plug-in buckle 97 and a small plug-in buckle 98. The large plug-in buckle 97 is used to plug and fix the outer sleeve 87, and the small plug-in buckle 98 is used to plug and fix the inner sleeve 92; the center position of the bottom cover 96 is provided with an intelligent vacuum water pump 99, a micro water pump A 100, a micro water pump B 101 and a control board 102; the bottom cover 96 is provided on the base 103, and the base 103 is also provided with a foot 104, and the bottom The seat 103 is divided into a storage area A 105, a storage area B 106 and a storage area C 107 for storing liquid reagents; the intelligent vacuum water pump 99, the micro water pump A 100, and the micro water pump B 101 are connected to the storage area A 105, the storage area B 106 and the storage area C 107 through a straw, and are connected to each of the arc-shaped nozzles 90 using a plastic tube. The control board 102 is electrically connected to the intelligent vacuum water pump 99, the micro water pump A 100, the micro water pump B 101, the color sensor 88, the arc-shaped screen 89 and the micro motor 93.

[0099] The wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip, wherein the device comprises the above-mentioned bowl-shaped chip 1, clamping disk 2, conical chip layer 3, bottom plate 4 and cover plate 5, wherein the bowl-shaped chip 1 is divided into a preset number of intervals in a ring reagent box 12, pre-infused with screening reagents, and is set by manually pressing the reagent channel and blowing air through the mouth in a positive pressure manner, and the wearable device 108 operates the switch button 109 to select the function switch, display screen and corresponding display and electric board 110, and the screening is achieved by pressing the wearable device 108 onto the vertical cylindrical wearable base 111 for vertical operation.

[0100] The bowl-shaped centrifugal microfluidic chip in the automated device (hereinafter referred to as bowl-shaped chip 1; see Figure 1 、 Figure 2 ), is to set a bowl-like bottom disc as the bottom disc 4 (see Figure 3 、 Figure 4 ) and cover plate 5 (see Figure 5 、 Figure 6 ) engaged with the clamping plate 2 (see Figure 7 ); a hollow conical chip layer having a microporous wall structure similar to a hollow cone on the edge of a bowl (hereinafter referred to as: conical chip layer 3; see Figure 8 、 Figure 9 ), the clamping plate 2 is provided with a liquid inlet channel 6 (see Figure 9 ), the liquid inlet channel 6 is composed of a circular reagent box 12, an arc reaction tank 13, a circular tube groove 14, a groove liquid guide tube 15, a circular tube cavity 25, a reverse wedge ring slot 26, and a liquid inlet socket 30; the conical chip layer 3 is provided with independent microporous array screening channels 7 arranged side by side (see Figure 10 ), the screening channel 7 is composed of the liquid inlet pipe 37, the diversion channel 38, the anti-backflow buckle 39, the pre-assembly port 40, the upper blocking channel 41, the lower blocking channel 42, the microsphere 43, the anti-fall buckle 44, the square color development tank 45, the waste liquid tank 46, the absorbent paper 47, the anti-overflow pipe 48 and the exhaust groove 49. The conical chip layer 3 is embedded in the clamping disk 2 to form a three-dimensional intersecting bowl-shaped chip 1, and a motor shaft is inserted in the center of the clamping disk 2 to achieve centrifugal mixing of the bowl-shaped chip 1.

[0101] The clamping plate chassis 4 is a flat bottom disc with a diameter of φ150 mm and a height of 18 mm (see Figure 3), a concave opening 8 with a depth of 2mm and a diameter of φ200mm is provided at the upper end of the chassis 4; a central cylinder 9 with a height of 12mm and a diameter of φ30mm is provided in the disk, and a straight through shaft hole 10 with a diameter of φ8mm is provided therein for plugging in the motor, and two positioning pin holes 11 with a diameter of φ3mm are provided on the edge of the shaft hole; and a circular ring reagent box 12 with a height of 9mm, a diameter of φ90mm at the upper end and a diameter of φ80mm at the lower end; an arc reaction groove 13 with a height of 10mm and a diameter of φ116mm, a circular tube groove 14 with a diameter of φ9mm, and a groove liquid guide tube 15 with a diameter of φ3mm below the circular tube groove 14; a chassis card slot 16 with a bayonet is provided on the upper circumferential edge of the chassis 4; an inwardly concave bottom 17 is provided at the bottom of the chassis 4; the positioning pin hole 11 and the chassis card slot 16 are used together for positioning and embedding the corresponding configuration on the clamping plate cover 5, the liquid inlet channel 6 of the bowl-shaped chip 1 (please refer to Figure 3 、 Figure 4 、 Figure 5 ) is arranged on the plane layer of the clamping plate 2 outside the bottom of the bowl, and the liquid inlet channel 6 is composed of a liquid inlet socket 30, a circular ring reagent box 12, an arc liquid storage tank 63, a circular tube groove 14, and a groove liquid guide tube 15 in sequence.

[0102] The annular reagent box 12 is configured to be closed on the inside, with a reagent box slope 19 on the outside circumference, the top of the box protruding outward, and the bottom tilted inward to prevent reagent splashing; the bottom surface 20 of the reagent box is configured to be high in the center and tilted low toward the circumference to facilitate reagent flow; the annular reagent box 12 is configured to be evenly divided into eight segments of annular cavities 21 according to angles, and the annular cavity 21 is further divided into three input reagents and a fan-shaped grid 22 for inputting gas.

[0103] The arc reaction tank 13 is also divided into eight arc cavities 23 in proportion, with a length / width / depth of 35 / 17 / 10 mm, which can accommodate 1 to 6 ml of reaction liquid. A virus screening primer 24 is pre-buried in each arc cavity 23 to detect its pathogens; then the circular tube groove 14 with a diameter of φ9 mm is also divided into eight circular tube cavities 25 and connected to the periphery of each arc cavity 23. A groove catheter 15 with a length of 3 mm and a diameter of φ2.5 mm is provided at the bottom of each circular tube cavity 25; a 10 mm wide inverted ring slot 26 is also provided below the groove catheter 15.

[0104] The clamping disk cover plate 5 is configured as a circular disk with an axial hole, and the axial hole of the cover plate 5 is equipped with an external hexagonal reverse thread nut 27 and a positioning pin 28 on the edge of the axial hole, and then a circular hole ring 29, a liquid inlet socket 30, a cover plate stop 31, and a cover plate buckle 32 are sequentially arranged outward. The nut, positioning pin 28, cover plate stop 31 and cover plate buckle 32 configured on the axial hole are used to accurately fit the cover plate 5 on the chassis 4 of the clamping disk 3; the circular hole ring 29 is used to insert multiple nozzles to input various reagents respectively, and to input positive pressure gas from the air pump 33; the liquid inlet socket 30 is used to insert the liquid inlet funnel 56 to input sample liquid.

[0105] The conical chip layer 3 of the microporous wall structure (see Figure 4 、 Figure 5 ), a coil of thermoplastic resin and the same size is provided as a chip base layer 34 of the conical chip layer 3, a PET coil is adhered to the outside of the chip base layer as a chip cover layer 35 of the chip layer, and a PE coil is adhered to the outside of the chip cover layer 35 as a chip protection layer 36 of the chip layer (in this case, only a unidirectional outer layer is used for the single-sided chip). The surface of the chip base layer 34 is respectively provided with eight groups of microstructured screening channels 7, and the structure of each screening channel 7 is sequentially configured as follows:

[0106] (1) Liquid inlet pipe 37 (φ3mm, length 5mm);

[0107] (2) diversion channels 38 (two channels with length, width and thickness of 5 / 3 / 2 mm respectively, forming a 45° angle);

[0108] (3) Anti-backflow buckles 39 (two widths and thicknesses of 0.4 / 0.4 mm respectively);

[0109] (4) Pre-assembly opening 40 (two diamond-shaped shapes with length, width and thickness of 5 / 5 / 1 mm respectively);

[0110] (5) Upper blocking channel 41 (width and thickness are 0.5 / 0.5 mm, 1 / 4 length is 4 mm)

[0111] (6) Lower blocking channel 42 (width / thickness 0.4 / 0.4 mm, 3 / 4 length 12 mm, lower half of the channel is the blocking section);

[0112] (7) microspheres 43 (30 μm in diameter, pre-filled from the pre-assembly port 40 for pre-blocking);

[0113] (8) Anti-fall buckle 44 (a buckle is provided to prevent the microsphere from falling);

[0114] (9) Square color development tank 45 (two length, width and thickness are 10 / 10 / 2 mm respectively, and can accommodate 0.2 ml of solution);

[0115] (10) Waste liquid tank 46 (length, width and thickness are 15 / 10 / 2 mm);

[0116] (11) Absorbent paper 47 (length, width and thickness are 8 / 5 / 0.3);

[0117] (12) overflow prevention pipe 48 (length, width and thickness are 12 / 2 / 2);

[0118] (13) Exhaust groove 49 (diameter: φ4 mm / thickness: 2 mm / height: 10 mm).

[0119] After the chip base layer 34 is covered with a PET chip cover layer 35 after processing, a QR code 50 is printed above the exhaust groove 49 of the chip base layer 34, and an exhaust hole 51 connected to the outside is punched out at the exhaust groove 49. The exhaust hole 51 is used to discharge the gas in the pipeline of the bowl-shaped chip 1 during the input, vibration, centrifugation and positive pressure processes of the liquid sample.

[0120] A transparent PE chip protection layer 36 is then laminated and glued onto the surface of the PET chip cover layer 35. Finally, the roll is cut, pressed, and curled to form a single 60mm wide roll, with a small hole end 53 approximately 130mm in diameter and a large hole end 52 approximately 160mm in diameter. A 10mm-long hot melt adhesive 54 is applied to the edge of the small hole end 53. Furthermore, a chip interface 55 is provided on the surface of the conical chip layer 3, running along the height of the conical chip 3. After adhesive coating, gluing, and curling, the roll forms the conical chip layer 3.

[0121] Then, the small hole end 53 of the conical chip layer 3 is inserted into the inverted ring slot 26 at the bottom of the clamping plate chassis 4, and the groove liquid guide tube 15 is correspondingly inserted into the liquid inlet pipe 37. Then, the hot melt adhesive 54 coated on the end surface of the small hole end 53 of the conical chip layer 3 is completely bonded to the inverted ring slot 26 at the bottom of the clamping plate chassis 4 to achieve matching assembly of the bowl-shaped chip 1.

[0122] The liquid inlet funnel 56 is set to have an oval open shape with an upper height of 15mm and a diameter of about 30mm. The middle part is funnel-shaped and the lower outlet end is a conical tube. The open end is provided with an oval reagent pack plug 61 of the same size that can be plugged into extruded plastic. The inner edge of the open end of the liquid inlet funnel 56 is provided with a puncture component 57, and the middle pad is provided with a fiber filter layer 58; the two sides of the lower cone tube of the liquid inlet funnel 56 are provided with fascia strips 59 of corresponding size to the liquid inlet socket and fixed at the upper part, and the lower part of the fascia strip 59 is a movable strip 60 for locking the two sides of the open funnel to prevent retreat; virus lysis solution is provided in the reagent pack plug 61, and the liquid inlet funnel 56 is used to collect samples of oral sputum. The puncture component 57 is used to insert and puncture the reagent pack plug 61, thereby infusing the virus lysis solution to flush the viscous sputum blocked by the fiber filter layer 58.

[0123] During screening, three reagent spray nozzles and one gas spray nozzle are inserted into the four circular holes in the circular hole ring 29 on the cover plate toward the center of the reagent cartridge, and then injected into the four sector-shaped grids 22. First, 30 μL of alcohol and 15 μL of ammonium acetate are sprayed into two of the sector-shaped grids. After centrifugation, 280 μL of red oil dye is sprayed in. After further centrifugation, positive pressure gas from an air pump is introduced through the nozzle inserted into the fourth sector-shaped grid 22.

[0124] Currently, epidemic research is primarily focused on testing for pneumonia caused by the novel coronavirus. However, a variety of viruses can cause respiratory infections, including respiratory syncytial virus, rhinovirus, adenovirus, metapneumovirus, influenza virus, parainfluenza virus, coronavirus, and coxsackie virus. To address the similar symptoms of viral influenza and the common cold, and to avoid the laborious, time-consuming, and costly nature of multiple tests and the risk of widespread transmission caused by missed virus detection, a combination of multiple respiratory virus detection reagents, particularly during cold weather, is crucial for rapid isolation and targeted treatment. This case proposes an alternative technical solution.

[0125] As another preferred embodiment of the present invention, please refer to Figure 13 、 Figure 14 As shown, the aforementioned method is based on the "nucleic acid physical precipitation blocked flow" technology and uses a bowl-shaped centrifugal microfluidic (on-site visual) chip (similar to the previous case and will not be repeated here). The difference is that this case divides the aforementioned ring test kit 12 into two inner and outer boxes:

[0126] 1. A small ring reagent box 62 is provided with a reduced single structure (three reagents and one gas channel) as its inner layer;

[0127] 2. The amount of reagents added to the small ring reagent kit 62 is increased (doubled according to the reagents in the first embodiment);

[0128] 3. A circular liquid storage tank 63 is added (set on the periphery of the small circular reagent box 62);

[0129] 4. Eight "primers and probes 64" targeting different respiratory viruses are added and embedded in eight different arc cavities 23;

[0130] 5. The same screening method as the first embodiment is adopted to achieve simultaneous screening of multiple target viruses on a single sample.

[0131] The embodiment of the present technical solution is based on the principle that "if the diameter of the precipitate is larger than the gap size of the microsphere accumulation, the oily colored indicator liquid can be visually detected as a positive test result after the detection layer blocks the channel under the action of positive pressure."

[0132] After introducing a mixture of ethanol and ammonium acetate into the bowl-shaped chip 1 in this case, the centrifuge motor was activated again to centrifuge for 30 seconds. During this centrifugation, the alcohol and the reaction system rapidly mixed, resulting in a uniform nucleic acid precipitate. To better visualize the reaction results, 280 μL of red oil-based dye was injected into the clamping disk 2, allowing for rapid visual inspection of the test results. Positive pressure was then applied to the bowl-shaped chip 1 at room temperature.

[0133] The bowl-shaped centrifugal microfluidic chip is applied as follows, and the technical features not described in detail are the same as those described above and will not be repeated here.

[0134] The bowl-shaped chip 1 provided in the first type of automated device of the present invention specifically includes the following operation process:

[0135] 1. Add an aerosol sampler 65 (the screener enters the room and the aerosol sampler 65 and the negative pressure exhaust fan 66 are immediately started);

[0136] 2. Sampling is completed by adding a liquid inlet funnel 56 (inserted into a disposable mask with a hole in the center), inserting a stopper (squeezing in the virus lysis solution) after blowing and spitting;

[0137] 3. After the screener completes a sampling and presses the fingerprint controller:

[0138] ① The bowl-shaped chip 1 is then rotated to an angle, and the screening window baffle 69 will reveal the unused liquid inlet socket 30;

[0139] ② After sampling, the liquid inlet funnel 56 wrapped with the disposable mask will be sterilized by heating and baking in the medium temperature sterilizer 68;

[0140] ③ If each sampling socket of the bowl-shaped chip 1 is used up, it will automatically rise and level off to enter the horizontal conveying state;

[0141] 4. When the bowl-shaped chip 1 reaches the horizontal transport position, the multi-head peristaltic pump 81 is immediately started and the annular nozzle 82 is lowered (here, there are 8 groups of 32 nozzles, of which each group contains 3 reagent nozzles and 1 gas nozzle) into the bowl-shaped chip 1. The annular nozzle 82 on the outside of the reagent box is inserted into the annular nozzle 82 and the reagent (the reagent contains 30 μl of a 2:1 mixed sputum solution of alcohol and ammonium acetate) is input into the arc reaction tank 13. The plug-in annular nozzle 82 is immediately raised and the mixture is rapidly centrifuged for 30 seconds. Then, 20 μL of oily colored indicator solution is flowed into each arc reaction tank and centrifuged for another 30 seconds.

[0142] 5. Start the micro air pump 80 to inflate the arc reaction groove of the bowl-shaped chip 1, generating positive pressure to drive the sample flow;

[0143] 6. Use the color sensor 88 (or visual inspection) to check whether the colors of the "reaction tank blocking section channel and the color development section channel" are abnormal.

[0144] After the bowl-shaped chip 1 is collected, the system automatically lifts and levels the bowl-shaped chip 1. The multi-head peristaltic pump immediately aligns with the circular hole 29 on the bowl-shaped chip 1 to input the corresponding reagent and rapidly rotates it centrifugally. After rotating and mixing the reagent for 30 seconds, it quickly stops. The multi-head peristaltic pump 81 again inputs the colored reagent and rotates rapidly for another 30 seconds before stopping. The micro air pump 80 quickly blows positive pressure gas into the bowl-shaped chip 1 and slowly rotates the bowl-shaped chip 1. The video camera screens the chip layer observation area on the side of the bowl-shaped chip 1 piece by piece to see if there is any color abnormality. Once an abnormality is found, the system will immediately upload the QR code of the abnormal area to the cloud server through a signal transmitter. The cloud server will parse, verify, and authenticate it, and issue a test report for the subject's new coronavirus nucleic acid reagent to the system center and the subject's mobile phone. At the same time, the system will also send an alarm through voice and light. If the system's negative pressure exhaust fan 66 detects virus information through the air (including epithelial cells and metabolites) sensor in the screening room, an alarm message will also be sent through a voice player and light.

[0145] A bowl-shaped wearable centrifugal (visual identification) microfluidic chip, the application of which includes the following steps:

[0146] Step 1: After the ID card and video verification, the subject will receive a specific "QR code" to open the door and enter the screening;

[0147] Step 2: Take a disposable mask (with a hole in the center) and insert the liquid inlet funnel 56 into the center hole of the disposable mask;

[0148] Step 2: Unplug the liquid inlet plug at the liquid inlet of the clamping plate, insert the liquid inlet funnel 56 with the mask and wear it on the face;

[0149] Step 3: Bite the liquid inlet funnel 56 and blow air first, then induce sputum, then insert the stopper and squeeze in the virus lysis solution;

[0150] Step 4: Take off the mask and tie it tightly to the liquid inlet funnel 56 with the ear rope, press the fingerprint controller and exit;

[0151] Step 5: The bowl-shaped chip 1 is rotated to reveal the unused liquid inlet plug, and the next person can continue sampling;

[0152] Step 6: After the sampling is completed, the bowl-shaped chip 1 is immediately raised and leveled, and enters the horizontal conveying screening process;

[0153] Step 7: The multi-head peristaltic pump inputs reagent into the circular hole ring 29 on the bowl-shaped chip 1 and rapidly centrifuges for 30 seconds;

[0154] Step 8: Use a multi-head peristaltic pump to release 30 μl of a 2:1 mixed sputum solution of alcohol and ammonium acetate into the arc reaction tank 13;

[0155] Step 9: Then, 20 μL of oily colored indicator solution was poured into each arc reaction tank and centrifuged for 30 seconds;

[0156] Step 10: Start the intelligent vacuum pump to inflate the arc reaction tank, generating positive pressure to drive the sample flow;

[0157] Step 11: Visually check whether the colors of the "blocked section channel and the color development section channel of the reaction tank" are abnormal.

[0158] The bowl-shaped chip 1 of the second desktop device 85 described in this case adopts the same size as the automated bowl-shaped chip, and adopts an intelligent vacuum pump and a water pump to extract from the bottom box and inject liquid from the top of the bowl-shaped chip. It is composed of a cylindrical outer sleeve 87, an inner sleeve 92 and a disc-shaped bottom box 86 stacked together. The inner wall of the outer sleeve 87 is provided with a color sensor 88, an arc-shaped screen 89 is attached to the arc of the outer wall, and the top is provided with arc-shaped nozzles 90 (four side by side) and an outer sleeve handle 91; the top center of the inner sleeve 92 is provided with a power supply and a micro motor shaft 94 protruding vertically upward. Above the micro motor shaft 94 is provided a positioning turntable 95 (rotating disk 95-1 / / fixed disk 95-2 at the bottom of the rotating disk / / positioning steel balls 95-3 installed on the fixed disk), and the bowl-shaped chip 1 is installed on the rotating disk 95-1;

[0159] The bottom box 86 includes a bottom cover 96 and a base 103. The bottom cover 96 is provided with a large plug-in buckle 97 and a small plug-in buckle 98. The large plug-in buckle 97 is used to plug and fix the outer sleeve 87, and the small plug-in buckle 98 is used to plug and fix the inner sleeve 92. The center of the bottom cover 96 is provided with an intelligent vacuum water pump 99, a micro water pump A 100 and a micro water pump B 101, and a control board 102. The base 103 is provided with a foot 104. The base is divided into three liquid reagent storage areas A 105. , storage area B 106, and storage area C 107; the intelligent vacuum water pump 99, micro water pump A 100 and micro water pump B 101 are connected to the three liquid reagent storage areas A 105, storage area B 106, and storage area C 107 by straws, and are connected to the four nozzles 90 arranged in an arc shape by plastic tubes; the control board 102 is electrically connected to the intelligent vacuum water pump 99, micro water pump A 100 and micro water pump B 101, the micro color sensor 88, the arc-shaped screen 89, and the micro motor 93.

[0160] The bowl-shaped chip 1 provided in the third wearable device 108 of this case is a chip with a diameter of φ50*80 and a height of 22 mm (please refer to Figure 25 、 26 , 27, 28, and 29), the test kit 12 is divided into four sections, pre-filled with three screening reagents, and the reagent channel is manually pressed through, and a positive pressure method is used by blowing air through the mouth, and the wearable device 108 and the switch button 109 select the function switch, the display screen and the control circuit and power supply behind it, and the wearable device is pressed on the vertical cylinder for vertical operation by using a vertical cylinder for screening.

[0161] The current large-scale collection of nasopharyngeal swabs for virus screening not only has a significant impact on screening results in terms of skills and infection period, but also is very time-consuming to heat and amplify the virus during testing. This case, taking advantage of the fact that the content of suspected viruses in sputum far exceeds that in the nasopharyngeal mucosa, uses sputum as a sample for virus screening. First, there is no need to heat and amplify the sample to be tested; second, there is no need for professional operators, which can reduce human errors; third, there is no aerosol contamination; it can significantly speed up screening and increase the specificity and sensitivity of detection. It only takes 3 minutes of mechanical operation to fully mix the solution, achieve accurate and even sample distribution after centrifugation, and positive pressure drive can quickly promote sample separation, thus achieving fully automatic operation and visual rapid detection of multiple targets in sputum samples.

[0162] In this case, the liquid inlet funnel 56 is used as a mouthpiece for blowing, and mustard powder is added to the surface of the honeycomb sponge 58 to cause stimulation and coughing. When the user blows, the airway will vibrate and produce a violent cough to loosen the secretions in the airway, making the thick sputum adhering to the airway loose and easy to discharge (it has the same expectoration effect as a vibrating expectorator), and pushing the loosened secretions (epithelial cells, metabolites) in the trachea and alveoli from bottom to top into the upper airway, thereby improving the lung environment and facilitating the coughing out of the secretions.

[0163] In this case, a honeycomb sponge is embedded in the liquid inlet funnel 56, relying on the large contact area of ​​the honeycomb sponge to efficiently block and absorb the viscous sputum in the sample. At the same time, with the help of the impact of squeezing the open end of the liquid inlet funnel 56 to insert the "virus lysis solution" in the reagent pack plug 61, the thin mucus sample in the sputum can flow smoothly through the gap to the liquid storage tank, thereby ensuring that the virus lysis solution dissolves and releases the viral nucleic acid, thereby achieving the successful collection of sputum samples.

[0164] In this case, absorbent paper is used in the waste liquid tank to absorb excess test liquid, thereby avoiding large-scale overflow of the test liquid and improving the safety of the sample.

[0165] This case uses a virus screening method that uses both facial images and ID cards, as well as a QR code on a bowl-shaped chip (1) to identify the subject. By linking the screening device's Bluetooth device with a mobile phone app, data can be uploaded and stored in an epidemic monitoring system on a network big data platform, and simultaneously uploaded to the user's mobile phone. The epidemic and virus data service system collects, organizes, filters, determines, analyzes, and provides feedback, sharing data in the cloud and quickly displaying it, providing detailed reference for the government's epidemic assessment. The introduction of big data will make screening devices a true extension of human monitoring capabilities, and their importance will undoubtedly grow. It will play a significant role in virus screening and epidemic monitoring.

[0166] The screening protocol using an "ethanol solution and nucleic acid" reagent was primarily based on the technology published in CN113652341A, which utilizes a combination of an ethanol solution, ammonium acetate, and an oily red indicator solution, along with a "simplified flow channel and visual inspection" approach. However, to enhance the efficiency and effectiveness of this protocol, a superior solution featuring "direct sample collection, pre-embedded reagents, pneumatic positive pressure," and a "bowl-shaped chip centrifuge package" was proposed. This significantly enhances existing technology and fully demonstrates its effectiveness.

[0167] At the same time, the chip adopts an integrated and disposable design, which uses a small amount of reagents and has a high degree of integration. The relevant primers and probes are pre-buried and adhered to the bowl-shaped conical chip. There is no cross-miscibility in the chip and no pollution to the environment. The centrifugal force in the chip can ensure that the sputum samples can be fully mixed and flow smoothly into each functional groove. The collection and detection of sputum samples can be completed quickly in a relatively closed chip, and the test results can be directly determined visually, which greatly improves the portability of the test and does not require the samples to be sent for inspection, thereby shortening time and improving efficiency. The bowl-shaped centrifugal microfluidic chip has the characteristics of being convenient and easy to make, fast and sensitive, stable and low-cost, highly sensitive, highly efficient, high-throughput, and highly automated. It improves the existing microfluidic chip's ability to manipulate fluids, realizes the detection and analysis of multifunctional microfluidic samples, has a very large market in expanding the application of microfluidic technology into the home, and has extremely good application value in the field of POCT testing.

[0168] Compared with the prior art, the beneficial effects brought about by the present invention are shown in the table:

[0169]

[0170]

[0171] Throughout this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "embodiment" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0172] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

[0173] The bowl-shaped centrifugal microfluidic chip of the present invention, which uses sputum to screen for viruses, integrates multiple reactions of conventional analytical detection methods into a single bowl-shaped centrifugal microfluidic chip, providing a non-invasive, efficient, and convenient method for collecting and testing sputum at airport customs around the clock, as well as for direct home use. It can be widely used to detect a variety of respiratory viruses, significantly improving the accuracy and speed of test results, as well as the screening rate for asymptomatic or early-stage patients, and avoiding the risk of cross-infection. Furthermore, the device is easy to operate and carry, simple to manufacture, efficient, economical, and durable, making it very suitable for large-scale promotion and application.

[0174] In this specification, the present invention has been described with reference to specific embodiments thereof. However, it will be apparent that various modifications and variations may be made without departing from the spirit and scope of the present invention. Accordingly, the specification and drawings are to be regarded as illustrative rather than restrictive.

Claims

1. A device for virus screening based on a bowl-shaped centrifugal microfluidic chip, characterized in that: The device comprises: A clamping plate (2) is provided by engaging the base plate (4) with the cover plate (5); A conical chip layer (3) matching the clamping disk (2) and configured as a hollow cone with a microporous wall structure; The conical chip layer (3) and the clamping disk (2) are interlocked to form a bowl-shaped chip (1) arranged in a three-dimensional intersecting shape; and a motor shaft is inserted into the center position of the clamping disk (2), and the motor shaft is used to achieve centrifugal mixing treatment for the bowl-shaped chip (1); The conical chip layer (3) is provided with a thermoplastic resin material having the same size as the chip base layer (34); The conical chip layer (3) is further provided with a single-sided chip layer or a double-sided chip layer; the single-sided chip layer is provided with at least four or more screening channels (7) only on the outer surface of the chip base layer (34); the double-sided chip layer is provided with at least four or more screening channels (7) on the inner and outer sides of the chip base layer (34), wherein each of the screening channels (7) is provided with a liquid inlet pipe (37) at the top of the conical chip layer (3), and the liquid inlet pipe (37) is connected to at least one or more separation channels. A flow channel (38) is provided in the diversion channel (38), and a backflow prevention buckle (39) is provided in the diversion channel (38). A pre-assembly port (40) is provided on the diversion channel (38) along the lower part of the anti-backflow buckle (39). The lower end of the pre-assembly port (40) is connected to the upper blocking channel (41) and the lower blocking channel (42) in sequence. The bottom of the lower blocking channel (42) is connected to the square color development tank (45), and the square color development tank (45) is connected to the waste liquid tank (46) through the anti-overflow pipe (48).

2. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 1, characterized in that: A layer of PET coil is adhered to the outside of the chip base layer (34) as a chip cover layer (35) of the conical chip layer (3), and a layer of PE coil is adhered to the outside of the chip cover layer (35) as a chip protection layer (36) of the conical chip layer (3); and A blotting paper (47) is also provided in the waste liquid tank (46), and an exhaust groove (49) is provided above the waste liquid tank (46). An exhaust hole (51) is also provided on the exhaust groove (49). The exhaust hole (51) is used to discharge the gas in the pipeline of the bowl-shaped chip (1) during the input, vibration, centrifugation and positive pressure processes of the liquid sample.

3. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 2, characterized in that: The chassis (4) is a flat-bottomed circular disc, and a concave opening (8) is provided at the upper end of the chassis (4). A central cylinder (9) is provided inside the chassis (4). The central cylinder (9) is provided with a straight-through shaft hole (10) for plugging in the motor, and a positioning pin hole (11) is provided on the edge of the straight-through shaft hole (10). The upper circumferential edge of the chassis (4) is provided with a chassis slot (16) with a bayonet, and the bottom of the chassis (4) is provided with an inwardly concave bottom, and the positioning pin hole (11) and the chassis slot (16) are both used to position and engage the cover plate (5) of the clamping plate (2); and The liquid inlet channel (6) of the clamping plate (2) is formed by a liquid inlet socket (30), a circular ring reagent box (12), an arc reaction groove (13), a circular tube groove (14) and a groove liquid guide tube (15), wherein the liquid inlet socket (30) is arranged on the cover plate (5), the circular ring reagent box (12) is arranged on the periphery of the central cylinder (9), the circular arc reaction groove (13) is arranged on the periphery of the circular ring reagent box (12), the circular tube groove (14) is arranged on the periphery of the circular arc reaction groove (13), and a plurality of the groove liquid guide tubes (15) are distributed on the circular tube groove (14).

4. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 3, characterized in that: The box body of the circular reagent box (12) is made of plastic, and its annular outer surface is configured with an upper end protruding outward and a lower end inclined inward, and is sealed by a film to prevent the reagent from splashing; The bottom of the circular reagent box (12) is configured as a structure with a high center and inclined in the circumferential direction. The circular reagent box (12) is configured according to an angle to have N independent arc segments, and each arc segment is divided into M independent fan grids. Each of the fan-shaped grilles (22) is pre-buried with one or more powders or reagents, as well as a channel for inputting gas. One end of the gas channel is connected to a micro air pump, and the other end is connected to the circular tube groove (14) and the groove through hole of the groove guide tube (15).

5. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 3, characterized in that: The arc reaction tank (13) is evenly and evenly provided with a plurality of arc cavities (23) in proportion to accommodate reagents and reaction liquids, and each of the arc cavities (23) is pre-embedded with at least one or more virus screening primers (24) for detecting pathogens; The circular tube groove (14) is evenly divided and arranged on the periphery of each section of the circular arc cavity (23) in the same proportion as the circular arc reaction groove (13), and the bottom of each section of the circular arc cavity (23) is provided with the groove liquid guide tube (15); a chisel ring slot (26) is also provided below the groove liquid guide tube (15).

6. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 5, characterized in that: The circular reagent box (12) is also configured as two box bodies distributed inside and outside, wherein the inner layer is configured as a small circular reagent box (62) with a reduced single structure, and the periphery of the small circular reagent box (62) is provided with a circular liquid storage tank (63) for increasing the amount of reagent added, and the periphery of the circular liquid storage tank (63) is also evenly provided with primers and probes (64), and each of the primers and probes (64) is embedded in the circular arc cavity (23) at the corresponding position.

7. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 1, characterized in that: The cover plate (5) is configured as a circular plate with an axial hole, and the axial hole of the cover plate (5) is provided with an external hexagonal reverse thread nut (27) and a positioning pin (28) provided at the edge of the axial hole; A circular hole ring (29), a liquid inlet socket (30), a cover plate stopper (31) and a cover plate buckle (32) are sequentially arranged outward along the axis hole. The hexagonal reverse nut (27), the positioning pin (28), the cover plate stopper (31) and the cover plate buckle (32) work together to precisely fit the cover plate (5) on the base plate (4) of the clamping plate (2); the circular hole ring (29) is used to insert multiple nozzles to input several reagents respectively, and to input positive pressure gas from the air pump (33); the liquid inlet socket (30) is used to be inserted into the liquid inlet funnel (56) to input the sample liquid.

8. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 7, characterized in that: The liquid inlet funnel (56) is configured to have an oval opening at the top, a funnel shape in the middle, and a conical through tube at the bottom outlet, and is used to collect oral sputum samples. The opening of the liquid inlet funnel (56) is provided with an oval reagent pack plug (61) of the same size that can be inserted into an extruded plastic reagent pack. A puncture component (57) is provided at the inner edge of the opening of the liquid inlet funnel (56), and a fiber filter layer (58) is provided in the middle position of the liquid inlet funnel (56). The liquid inlet funnel (56) The two sides of the lower conical tube are provided with fascia strips (59) corresponding to the size of the liquid inlet socket (30) and fixedly arranged on the upper part of the conical tube. The lower part of the fascia strips (59) is a movable strip block, which is used to lock and prevent the left and right sides of the liquid inlet funnel (56) from retreating; the reagent pack plug (61) is provided with virus lysis liquid, and the puncture member (57) is used to insert and puncture the extruded plastic reagent pack, thereby infusing the virus lysis liquid to flush the viscous sputum blocked by the fiber filter layer (58).

9. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 5, characterized in that: The chip cover layer (35) is manufactured by cutting, pressing and curling to have a plurality of large hole ends (52) and small hole ends (53) of preset sizes, each of the large hole ends (52) and small hole ends (53) being respectively arranged at the upper closed circular end and the lower closed circular end of the conical chip layer (3), and each of the large hole ends (52) and small hole ends (53) are set to a closed state; At least four liquid inlet pipes coated with a hot-melt quick-drying adhesive layer are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end (53); each section of the conical chip layer (3) is also provided with a group of cuts coated with a hot-melt quick-drying adhesive along the height direction of the conical chip layer (3); each section of the conical chip layer (3) is curled after the cuts are butt-jointed and bonded to form each section of the conical chip layer (3); The small hole end (53) of the conical chip layer (3) is inserted into the inverted ring slot (26), and the groove liquid guide tube (15) is inserted into the corresponding liquid inlet tube (37). The end surface of the small hole end (53) of the conical chip layer (3) is coated with hot melt adhesive (54), and the hot melt adhesive (54) is used to bond the inverted ring slot (26) set on the bottom of the chassis (4) to achieve matching assembly of the bowl-shaped chip (1).

10. The device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 1, characterized in that: A polymer water-absorbing material is pre-buried in the waste liquid tank (46), and microspheres (43) are provided at the position of the pre-assembly port (40) of the upper blocking channel (41) and the lower blocking channel (42). The microspheres (43) are used to prevent the reagent from being blocked, and anti-fall buckles (44) are provided at the bottom of the upper blocking channel (41) and the lower blocking channel (42). The anti-fall buckles (44) are used to prevent the microspheres (43) from falling.

11. An automated device for virus screening based on a bowl-shaped centrifugal microfluidic chip, characterized in that: The automated device includes the device for virus screening based on a bowl-shaped centrifugal microfluidic chip as described in claim 1, wherein the bowl-shaped chip (1) is injected with liquid from the top of the bowl-shaped chip (1) using a multi-head peristaltic pump, and the automated device is also provided with a liquid inlet funnel (56), an aerosol sampler (65), a negative pressure exhaust fan (66), a medium-temperature sterilization box (68) and a screening window baffle (69). When the screener enters the room, the aerosol sampler (65) and the negative pressure exhaust fan (66) are immediately started, the liquid inlet funnel (56) is inserted into a disposable mask with a hole in the center, and the stopper is inserted after blowing and spitting, and the virus lysis solution is squeezed in to complete the sampling; after the screener completes a sampling and presses the fingerprint controller, the bowl-shaped chip (1) is immediately rotated at an angle, and the screening window baffle (69) will reveal an unused liquid inlet port (30); after sampling, the liquid inlet funnel (56) wrapped with the disposable mask will perform the heating and baking sterilization procedure of the medium-temperature sterilization box (68).

12. A desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip, characterized in that: The device comprises the device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip as claimed in claim 1, wherein the bowl-shaped chip (1) is extracted from a bottom box by an intelligent vacuum pump and a water pump, and liquid is injected from the top of the bowl-shaped chip (1), the bowl-shaped chip (1) is composed of a cylindrical outer sleeve (87), an inner sleeve (92) and a disc-shaped bottom box (86) stacked together, a color sensor (88) is provided on the inner wall of the outer sleeve (87), and an arc-shaped screen (89) is attached to the arc of the outer wall of the outer sleeve (87), and a preset number of arc-shaped spray nozzles are provided on the top of the outer sleeve (87). A nozzle (90) and an outer sleeve handle (91); a micro motor (93) is provided at the top center of the inner sleeve (92), and a micro motor shaft (94) is provided in the vertical upward protruding direction of the micro motor (93); a positioning turntable (95) is provided above the micro motor shaft (94), and the positioning turntable (95) includes a rotating disk (95-1), a fixed disk (95-2) provided at the bottom of the rotating disk (95-1), and a positioning steel ball (95-3) installed on the fixed disk (95-2); the bowl-shaped chip (1) is installed on the rotating disk (95-1).

13. The desktop device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to claim 12, characterized in that: The bottom box (86) includes a bottom cover (96) and a base (103), and the bottom cover (96) is provided with a large plug-in buckle (97) and a small plug-in buckle (98), the large plug-in buckle (97) is used to plug and fix the outer sleeve (87), and the small plug-in buckle (98) is used to plug and fix the inner sleeve (92); the center position of the bottom cover (96) is provided with an intelligent vacuum water pump (99), a micro water pump A (100), a micro water pump B (101) and a control board (102); the bottom cover (96) is provided on the base (103), and the base (103) is also provided with a foot (104), and the base (103) is provided with a foot (104). ) is divided into a storage area A (105), a storage area B (106) and a storage area C (107) for storing liquid reagents; the intelligent vacuum water pump (99), the micro water pump A (100) and the micro water pump B (101) are connected to the storage area A (105), the storage area B (106) and the storage area C (107) through a straw, and are connected to each of the arc-shaped nozzles (90) using a plastic tube, and the control board (102) is electrically connected to the intelligent vacuum water pump (99), the micro water pump A (100), the micro water pump B (101), the color sensor (88), the arc-shaped screen (89) and the micro motor (93).

14. A wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip, characterized in that: The device comprises the device for virus screening based on a bowl-shaped centrifugal microfluidic chip as claimed in claim 3, wherein the bowl-shaped chip (1) is pre-filled with screening reagents by dividing the area into a preset number in a ring reagent box (12), and is set by manually pressing the reagent channel and blowing air through the mouth in a positive pressure manner, and the wearable device (108) operates the switch button (109) to select the function switch, display screen and corresponding display and electric board (110), and the screening is achieved by pressing the wearable device (108) onto a vertical cylindrical wearable base (111) for vertical operation.

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