Emamectin benzoate agent for preventing and treating pine wood nematode disease and preparation method thereof

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a emamectin benzoate agent for preventing and treating pine wood nematode disease and a preparation method thereof. An emamectin benzoate agent for preventing and treating pine wood nematode disease, wherein the raw materials for preparing the emamectin benzoate agent include at least 1-3wt% of emamectin benzoate, 5-30wt% of a surfactant, and 1-20wt% of an auxiliary agent by weight percentage, and the remainder is a solvent, wherein the solvent is a single compound and / or a mixture, and the relative molecular weight of each compound in the solvent is ≥18. In the technical scheme, the emamectin benzoate agent has an excellent effect of preventing and treating pine wood nematode disease for pine trees, especially masson pine. The inventor makes the emamectin benzoate agent have good storage stability, high and low temperature stability, good defoaming property, and can make the pine trees absorb and transport to each branch better, so as to achieve a long-term and efficient effect of preventing and treating pine wood nematode disease.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to an abamectin agent for controlling pine wilt disease and its preparation method. Background Technology

[0002] Pine wilt disease, caused by the pine wilt nematode, is a devastating forest disease and a major invasive alien species in my country. It has been listed as a quarantine pest for forest plants both domestically and internationally. In nature, many pine species are susceptible to pine wilt disease, though the specific species affected vary in different countries and regions. The spread of pine wilt disease primarily relies on insect vectors, with the pine sawyer beetle being the main vector. These beetles feed on healthy pine trees to obtain nutrients and spread the nematodes, or they spread the nematodes by laying eggs on weakened or dead pine trees. This is especially true for the widely distributed Masson pine in my country. Masson pine has straight grain, a coarse texture, good elasticity, and is rich in resin; its trunk can be tapped for resin, giving it high economic value. However, once infected with pine wilt disease, Masson pine has an extremely high mortality rate, causing severe economic losses.

[0003] Patent application CN201210369508.X discloses a photodegradation-resistant and antioxidant suspension of emamectin benzoate. This suspension has certain insecticidal activity and low toxicity to humans and animals; however, its stability and environmental applicability are poor. Patent application CN202010962227.X discloses a novel emamectin benzoate cyclodextrin supramolecular pesticide reagent and its preparation method. This method involves mixing an emamectin benzoate solution and a cyclodextrin aqueous solution, encapsulating the emamectin benzoate solution with cyclodextrin to reduce its photodegradation rate. However, the pesticide released by this method is too slow, reducing its control effect on pine wilt nematodes.

[0004] Existing pesticides for controlling pine wilt disease are not very effective against pine nematodes, especially against Masson pine. Summary of the Invention

[0005] The following is a part of the detailed embodiments of the present invention. Any substitution or change of simple components should be regarded as the same technical solution as the following embodiments. The present invention can adopt various alternative changes and steps, unless otherwise specified.

[0006] To address the aforementioned technical problems, the first aspect of this invention provides an abamectin agent for controlling pine wilt disease, wherein the raw materials for its preparation, by weight percentage, include at least 1-3 wt% of abamectin benzoate, 5-30 wt% of a surfactant, 1-20 wt% of an adjuvant, and the balance being a solvent, wherein the solvent is a single compound and / or a mixture, and the relative molecular weight of each compound in the solvent is ≥18.

[0007] Emamectin benzoate, also known as emamectin benzoate, kills pine wood nematodes through stomach poisoning and contact action, without harming beneficial insects. Emamectin benzoate targets the γ-aminobutyric acid (GABA) receptors in the peripheral nervous system, stimulating the release of GABA from nerve terminals and enhancing its binding to synaptic receptors on the postsynaptic membranes of inhibitory motor neurons and the postconjugative receptors on the muscle cells of pine wood nematodes. This enhanced GABA binding leads to increased Cl- influx into the cell, followed by hyperpolarization and removal of signal transduction, resulting in inhibition of nerve conduction and ultimately paralysis and death of the insect. However, the inventors found that when the emamectin benzoate concentration is too low, the amount of active ingredient is too small to achieve a good control effect against pine wood nematodes; conversely, a high concentration of active ingredient also fails to provide adequate control. The inventors believe the possible reason is that emamectin benzoate contains a large number of photolytically active sites, making it prone to photolysis during storage and use, thus reducing its efficacy. The inventors unexpectedly discovered that when the emamectin benzoate content is 2 wt%, it exhibits better control efficacy and a lower photolysis rate. The inventors believe this is likely because at higher concentrations, the emamectin benzoate solution can directly absorb visible light, leading to rapid and direct photolysis. At lower concentrations, the emamectin benzoate molecules have a larger dispersion distance in the solution, thus avoiding rapid and direct photolysis, resulting in a slower photolysis rate, longer efficacy, and a longer control cycle for pine wilt disease.

[0008] As a preferred technical solution, the solvent molecules all include at least one hydroxyl group.

[0009] As a preferred technical solution, when the solvent is a single compound, the relative molecular weight of the single compound is ≥30.

[0010] As a preferred technical solution, when the solvent is a mixture, at least one compound in the mixture has a relative molecular weight ≤25.

[0011] As a preferred technical solution, the solvent is selected from at least one of methanol, ethanol, 1-butanol, 2-butanol, 2-methyl-1-propanol, acetone and water.

[0012] As a preferred technical solution, the hydrophobic alkyl chain in the surfactant is a branched alkyl chain and / or a straight-chain alkyl chain.

[0013] As a preferred technical solution, the surfactant is a nonionic surfactant and / or anionic surfactant.

[0014] As a preferred technical solution, the HLB value of the nonionic surfactant is between 5 and 18.

[0015] As a preferred technical solution, the HLB value of the nonionic surfactant is between 7 and 15.

[0016] As a preferred technical solution, the nonionic surfactant is a polyoxyethylene sorbitan anhydride and / or a polyoxyethylene fatty alcohol ether.

[0017] As a preferred technical solution, the nonionic surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether, wherein the mass ratio of the polyoxyethylene sorbitan to the polyoxyethylene fatty alcohol ether is (1-3):1.

[0018] As a preferred technical solution, the polyoxyethylene sorbitan is selected from at least one of polyoxyethylene sorbitan tristearate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan trioleate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monopalmitate, and polyoxyethylene sorbitan monolaurate.

[0019] As a preferred technical solution, the polyoxyethylene fatty alcohol ether is selected from at least one of AEO3, AEO5, AEO7 and AEO9.

[0020] The inventors discovered that using nonionic surfactants with HLB values ​​between 5 and 18, especially polyoxyethylene sorbitan-based nonionic surfactants with HLB values ​​between 7 and 15, can improve the solubility and stability of emamectin benzoate in solvents, wherein the solvent is any of the solvents described in this invention. When the solvent contains water, emamectin benzoate has poor solubility in water. The lipophilic end of the polyoxyethylene sorbitan-based nonionic surfactant wraps around the emamectin benzoate molecule, forming an oil-in-water structure. However, under low-temperature conditions, the agent exhibits poor stability, resulting in aggregation and stratification. Furthermore, the inventors found that when using polyoxyethylene sorbitan-based nonionic surfactants, the agent's penetration is poor, preventing plants from fully and quickly absorbing the agent. The inventors unexpectedly discovered that when polyoxyethylene sorbitan-based surfactants are mixed with polyoxyethylene fatty alcohol ethers, not only is the low-temperature stability of the agent improved, but its penetration is also enhanced. The inventors believe the likely reason is that the hydrophilic groups of polyoxyethylene fatty alcohol ethers are linear chains. When polyoxyethylene fatty alcohol ethers are wrapped around the outside of emamectin benzoate molecules, the hydrophilic groups extend into the solution, reducing entanglement and collisions between oil droplets. Furthermore, the hydrophilic groups can penetrate the network of hydrophilic groups within the polyoxyethylene sorbitan, further reducing collisions. Additionally, due to the low steric hindrance and fewer branched chains of polyoxyethylene fatty alcohol ethers, plants can easily absorb them and transport them effectively to all parts of the plant, thus providing good control of pine wilt disease across all plant parts. The inventors also found that, especially when AEO9 is used in combination with polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monostearate, it also exhibits good high-temperature resistance and prevents stratification. However, when AEO3, AEO5, and AEO7 are used in combination with polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monostearate, the high-temperature stability of the agent is poor. Furthermore, the inventors discovered that the addition of polyoxyethylene sorbitan derivatives and polyoxyethylene fatty alcohol ethers causes foaming in the agent, affecting its use and reducing its ease of use.

[0021] As a preferred technical solution, the additives include at least one of dispersants, penetrants, wetting agents, antifreeze agents, defoamers, light stabilizers, and thickeners.

[0022] As a preferred technical solution, the defoamer is an alcohol ether, and the alcohol ether is at least one of diethylene glycol butyl ether, ethylene glycol butyl ether, diethylene glycol ethyl ether, ethylene glycol ethyl ether, and polyether polyol.

[0023] As a preferred technical solution, the defoamer is diethylene glycol butyl ether.

[0024] As a preferred technical solution, the defoamer is a mixture of diethylene glycol butyl ether and polyether polyol, wherein the mass ratio of diethylene glycol butyl ether to polyether polyol is (2-5):1.

[0025] As a preferred technical solution, the polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 2800-3000.

[0026] Foam is a dispersion system in which gas is dispersed in a liquid. The gas is the dispersed phase, i.e., the discontinuous phase, and the liquid is the dispersion medium, i.e., the continuous phase. Gas bubbles rise to the surface of the liquid, forming a small amount of liquid-based gas bubble aggregate separated by a liquid film. The inventors discovered that adding a certain amount of diethylene glycol butyl ether can reduce small bubbles in the pharmaceutical preparation to some extent and improve the low-temperature stability of emamectin benzoate in the solvent. However, the inventors found that diethylene glycol butyl ether does not effectively defoam larger bubbles. Unexpectedly, when a polyether polyol with a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 2800-3000 is combined with diethylene glycol butyl ether, it not only effectively eliminates small bubbles but also effectively eliminates large bubbles, and also has good foam suppression, improves the low-temperature resistance of the pharmaceutical preparation, and enhances the stability of the pharmaceutical preparation. However, if the hydroxyl value of polyether polyol is too low, it will reduce the high-temperature resistance of the agent. A low number-average molecular weight results in poor inhibition of large bubbles, while a high number-average molecular weight reduces the efficacy of the agent. The inventors believe that diethylene glycol butyl ether, due to its small molecular weight and good diffusivity, can penetrate into the interior of bubbles, thereby disrupting the gas-liquid balance within the bubble and thus eliminating small bubbles. Polyether polyol, on the other hand, has a larger molecular weight, better amphiphilicity, and a higher positive spreading coefficient, allowing it to spread well on the surface of large bubbles, thereby disrupting their stability. Therefore, diethylene glycol butyl ether and polyether polyol can effectively eliminate bubbles in the agent, improve its wettability, and promote its penetration into the plant.

[0027] As a preferred technical solution, the light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, wherein the mass ratio of wgwinD902 to butylated hydroxyanisole is 1:1.

[0028] To improve the photolysis resistance of the agent, the inventors added a certain amount of wgwinD902. However, they found that the photolysis resistance effect of wgwinD902 did not meet expectations. Through numerous experiments, the inventors unexpectedly discovered that wgwinD902 and butylated hydroxyanisole could give the abamectin agent better photolysis and antioxidant properties.

[0029] As a preferred technical solution, the thickener is gum arabic.

[0030] As a preferred technical solution, the abamectin agent for controlling pine wilt disease described in this invention is an oil-based agent. The raw materials for preparing the oil-based agent, by weight percentage, include at least 1-3 wt% of abamectin benzoate, 15-20 wt% of surfactant, 10-15 wt% of adjuvants, and the balance being a solvent, wherein the solvent is a single compound.

[0031] As a preferred technical solution, the surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether, wherein the mass ratio of the polyoxyethylene sorbitan to the polyoxyethylene fatty alcohol ether is (1-3):1.

[0032] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate, and the HLB value of the polyoxyethylene sorbitan monooleate is 15.

[0033] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monostearate, and the HLB value of the polyoxyethylene sorbitan monostearate is 14.9.

[0034] As a preferred technical solution, the polyoxyethylene fatty alcohol ether is AEO9, and the HLB value of AEO9 is 12.5.

[0035] As a preferred technical solution, the additive is a defoamer, and the defoamer is diethylene glycol butyl ether.

[0036] As a preferred technical solution, the solvent is methanol.

[0037] As a preferred technical solution, the solvent is ethanol.

[0038] As a preferred technical solution, the abamectin agent for the prevention and control of pine wilt disease described in this invention is a microemulsion. The raw materials for preparing the microemulsion, by weight percentage, include at least 1-3 wt% of abamectin benzoate, 25-30 wt% of surfactant, 15-20 wt% of adjuvants, and the balance being a solvent, wherein the solvent is a mixture.

[0039] As a preferred technical solution, the surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether, wherein the mass ratio of the polyoxyethylene sorbitan to the polyoxyethylene fatty alcohol ether is (1-3):1.

[0040] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate, and the HLB value of the polyoxyethylene sorbitan monooleate is 15.

[0041] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monostearate, and the HLB value of the polyoxyethylene sorbitan monostearate is 14.9.

[0042] As a preferred technical solution, the polyoxyethylene fatty alcohol ether is AEO9, and the HLB value of AEO9 is 12.5.

[0043] As a preferred technical solution, the additives include defoamer, light stabilizer and thickener, and the mass ratio of the defoamer, light stabilizer and thickener is (7-10):(0.1-0.5):(0.01-0.05).

[0044] As a preferred technical solution, the defoamer is a mixture of diethylene glycol butyl ether and polyether polyol, wherein the mass ratio of diethylene glycol butyl ether to polyether polyol is (2-5):1.

[0045] As a preferred technical solution, the polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 2800-3000.

[0046] As a preferred technical solution, the light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, wherein the mass ratio of wgwinD902 to butylated hydroxyanisole is 1:1.

[0047] As a preferred technical solution, the thickener is gum arabic.

[0048] As a preferred technical solution, the solvent is a mixture of methanol and water, wherein the mass ratio of methanol to water is (1-2):(15-20).

[0049] As a preferred technical solution, the solvent is a mixture of methanol and water, and the mass ratio of ethanol to water is (1-2):(15-20).

[0050] As a preferred technical solution, the abamectin agent for controlling pine wilt disease described in this invention is an emulsion. The raw materials for preparing the emulsion, by weight percentage, include at least 1-3 wt% abamectin benzoate, 20-25 wt% a surfactant, 10-15 wt% an adjuvant, and the balance being a solvent, wherein the solvent is a mixture.

[0051] As a preferred technical solution, the surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether, wherein the mass ratio of polyoxyethylene sorbitan to polyoxyethylene fatty alcohol ether is (1-3):1.

[0052] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate, and the HLB value of the polyoxyethylene sorbitan monooleate is 15.

[0053] As a preferred technical solution, the polyoxyethylene sorbitan is polyoxyethylene sorbitan monostearate, and the HLB value of the polyoxyethylene sorbitan monostearate is 14.9.

[0054] As a preferred technical solution, the polyoxyethylene fatty alcohol ether is AEO9, and the HLB value of AEO9 is 12.5.

[0055] As a preferred technical solution, the additives include defoamer, light stabilizer and thickener, and the mass ratio of the defoamer, light stabilizer and thickener is (10-13):(0.1-0.5):(0.1-0.3).

[0056] As a preferred technical solution, the defoamer is a mixture of diethylene glycol butyl ether and polyether polyol, wherein the mass ratio of diethylene glycol butyl ether to polyether polyol is (2-5):1.

[0057] As a preferred technical solution, the polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 2800-3000.

[0058] As a preferred technical solution, the light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, wherein the mass ratio of wgwinD902 to butylated hydroxyanisole is 1:1.

[0059] As a preferred technical solution, the thickener is gum arabic.

[0060] As a preferred technical solution, the solvent is a mixture of methanol and water, wherein the mass ratio of methanol to water is 1:(3-5).

[0061] As a preferred technical solution, the solvent is a mixture of methanol and water, and the mass ratio of ethanol to water is 1:(3-5).

[0062] A second aspect of the present invention provides a method for preparing the emamectin benzoate agent for the prevention and control of pine wilt disease, comprising at least the step of physically mixing emamectin benzoate, surfactant, adjuvant and solvent.

[0063] When using this invention, the medicine needs to be injected into the syringe container, a deep hole is drilled in the pine tree, the syringe container is inserted into the deep hole to prevent the medicine from leaking out, and finally a small opening is made at the bottom of the syringe container to allow air to enter and create pressure, so that the medicine continues to flow into the pine tree, achieving a preventive effect.

[0064] In addition, unless otherwise stated, all raw materials used are commercially available.

[0065] Beneficial effects: The abamectin agent in this technical solution has excellent control effects on pine trees, especially Masson pine, against pine wilt disease. Through the rational compounding of the components, the inventors have achieved good storage stability, high and low temperature stability, and good defoaming properties for the abamectin agent. Furthermore, it allows for better absorption and transport by the pine tree to all branches, achieving long-term and highly effective control of pine wilt disease. Detailed Implementation

[0066] Example 1

[0067] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is an oil-based formulation. The raw materials for preparing the oil-based formulation, by weight percentage, include 2 wt% emamectin benzoate, 20 wt% surfactant, 15 wt% adjuvant, and the balance being a solvent, specifically methanol. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a mass ratio of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, purchased from Xingtai Xinlanxing Technology Co., Ltd. The adjuvant is an antifoaming agent, specifically diethylene glycol butyl ether.

[0068] The second aspect of this embodiment provides a method for preparing the emamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing emamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the emamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0069] Example 2

[0070] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is an oil-based formulation. The raw materials for preparing the oil-based formulation, by weight percentage, include 3 wt% emamectin benzoate, 20 wt% surfactant, 15 wt% adjuvant, and the balance being a solvent, specifically methanol. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a mass ratio of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, purchased from Xingtai Xinlanxing Technology Co., Ltd. The adjuvant is an antifoaming agent, specifically diethylene glycol butyl ether.

[0071] The second aspect of this embodiment provides a method for preparing the emamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing emamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the emamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0072] Example 3

[0073] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is a water-in-oil emulsion. The raw materials for preparing the emulsion, by weight percentage, include 2 wt% emamectin benzoate, 20 wt% surfactant, 10 wt% adjuvants, and the balance being a solvent. The solvent is a mixture of methanol and water, with a mass ratio of methanol to water of 1:3. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a mass ratio of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, and AEO9 was purchased from Xingtai Xinlanxing Technology Co., Ltd. The additives include an antifoaming agent, a light stabilizer, and a thickener, with a mass ratio of 10:0.1:0.1. The antifoaming agent is a mixture of diethylene glycol butyl ether and polyether polyol, with a mass ratio of 2:1. The polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 3000. The polyether polyol was purchased from Guangzhou Huida Chemical Co., Ltd., product number MN-30500F. The light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, with a mass ratio of 1:1. The wgwinD902 was purchased from Shenzhen Langtai Biotechnology Co., Ltd. The thickener is gum arabic, purchased from Qingdao Haiweisen Biotechnology Co., Ltd., model number 212045.

[0074] The second aspect of this embodiment provides a method for preparing the emamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing emamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the emamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0075] Example 4

[0076] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is a water-in-oil emulsion. The raw materials for preparing the emulsion, by weight percentage, include 3 wt% emamectin benzoate, 25 wt% surfactant, 15 wt% adjuvants, and the balance being a solvent. The solvent is a mixture of methanol and water, with a methanol-to-water mass ratio of 1:3. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether mass ratio of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, and AEO9 was purchased from Xingtai Xinlanxing Technology Co., Ltd. The additives include an antifoaming agent, a light stabilizer, and a thickener, with a mass ratio of 10:0.1:0.1. The antifoaming agent is a mixture of diethylene glycol butyl ether and polyether polyol, with a mass ratio of 2:1. The polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 3000. The polyether polyol was purchased from Guangzhou Huida Chemical Co., Ltd., product number MN-30500F. The light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, with a mass ratio of 1:1. The wgwinD902 was purchased from Shenzhen Langtai Biotechnology Co., Ltd. The thickener is gum arabic, purchased from Qingdao Haiweisen Biotechnology Co., Ltd., model number 212045.

[0077] The second aspect of this embodiment provides a method for preparing the emamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing emamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the emamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0078] Example 5

[0079] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is a water-in-oil emulsion. The raw materials for preparing the emulsion, by weight percentage, include 2 wt% emamectin benzoate, 25 wt% surfactant, 15 wt% adjuvants, and the balance being a solvent. The solvent is a mixture of methanol and water, with a methanol-to-water mass ratio of 1:3. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether mass ratio of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, and AEO9 was purchased from Xingtai Xinlanxing Technology Co., Ltd. The additives include an antifoaming agent, a light stabilizer, and a thickener, with a mass ratio of 10:0.1:0.1. The antifoaming agent is a mixture of diethylene glycol butyl ether and polyether polyol, with a mass ratio of 2:1. The polyether polyol has a hydroxyl value of 26.5-29.5 mgKOH / g and a number-average molecular weight of 6000. The polyether polyol was purchased from Guangzhou Huida Chemical Co., Ltd., product number EP-3600. The light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, with a mass ratio of 1:1. The wgwinD902 was purchased from Shenzhen Langtai Biotechnology Co., Ltd. The thickener is gum arabic, purchased from Qingdao Haiweisen Biotechnology Co., Ltd., model number 212045.

[0080] The second aspect of this embodiment provides a method for preparing the emamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing emamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the emamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0081] Example 6

[0082] To address the aforementioned technical problems, the abamectin agent for controlling pine wilt disease provided in the first aspect of this embodiment is a water-in-oil emulsion. The raw materials for preparing the emulsion, by weight percentage, include 2 wt% emamectin benzoate, 25 wt% surfactant, 15 wt% adjuvants, and the balance being a solvent. The solvent is a mixture of methanol and water, with a methanol-to-water mass ratio of 1:3. The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ethers, with a polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether mass ratio of 1:1. The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate with an HLB value of 15. The polyoxyethylene fatty alcohol ether is AEO9 with an HLB value of 12.5, and AEO9 was purchased from Xingtai Xinlanxing Technology Co., Ltd. The additives include an antifoaming agent, a light stabilizer, and a thickener, with a mass ratio of 10:0.1:0.1. The antifoaming agent is a mixture of diethylene glycol butyl ether and polyether polyol, with a mass ratio of 2:1. The polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number-average molecular weight of 2000. The polyether polyol was purchased from Guangzhou Huida Chemical Co., Ltd., product number DL-20000. The light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, with a mass ratio of 1:1. The wgwinD902 was purchased from Shenzhen Langtai Biotechnology Co., Ltd. The thickener is gum arabic, purchased from Qingdao Haiweisen Biotechnology Co., Ltd., model number 212045.

[0083] The second aspect of this embodiment provides a method for preparing the abamectin benzoate agent for controlling pine wilt disease, comprising the steps of physically mixing abamectin benzoate, a surfactant, an adjuvant, and a solvent. When using the abamectin benzoate agent for controlling pine wilt disease in this embodiment, the agent needs to be injected into an injection bottle. The operator first drills a hole at an angle downwards in the pine trunk, then removes the nozzle from the injection bottle and inserts it into the trunk, ensuring a tight seal between the nozzle and the trunk to prevent leakage. Finally, the vent at the bottom of the injection bottle is opened to allow air to enter and create pressure, causing the agent to be conducted into the tree, achieving a preventative effect.

[0084] Performance testing

[0085] Performance Test 1

[0086] Efficacy Test: Thirty-three Masson pine trees with a diameter at breast height (DBH) of 10-30 cm were selected. Thirty of these trees were divided into two groups: Experimental Group 1 and Experimental Group 2. The remaining three trees served as a control group and were not injected with any agent. Experimental Group 1 and Experimental Group 2 were injected with the agents described in Examples 1 and 4, respectively. Five months later, the cultured pine nematodes were prepared into 33 1ml suspensions. The suspensions were shaken well, and the number of pine nematodes in each drop was measured, ensuring approximately 10,000 nematodes per milliliter. The selected Masson pine trees were inoculated with pine nematodes. Holes were drilled 1m above the injection site. The pine nematode suspension was shaken well, and 1ml was pipetted into the hole. The hole was then plugged with cotton. The growth of the Masson pine trees was assessed three months later.

[0087] Did the Masson pine die? Masson pine tree died Example 1 none 0 Example 4 none 0 control group have 3 trees

[0088] Performance Test 2

[0089] High temperature stability: 50 mL of the reagents obtained in Examples 1, 4 and 6 were poured into beakers, and then the beakers were placed in an oven at 55°C. After 20 days, the beakers were taken out and observed to see if they separated into layers.

[0090] Low temperature stability: 50 mL of the reagents obtained in Examples 1, 4 and 6 were poured into beakers, and then the beakers were placed in a refrigerator at -5°C. After 20 days, the beakers were taken out and observed to see if they separated into layers.

[0091]

[0092]

[0093] Performance Test 3

[0094] Take 100ml of the agent from Examples 4, 5, and 6 and put it into different 200ml graduated volumetric flasks. Seal the volumetric flasks and invert them 180° 20 times. Measure and record the volume of foam produced. If the volume of foam is ≤5ml, record it as qualified for defoaming; otherwise, record it as unqualified.

[0095] Defoaming properties Example 4 qualified Example 5 Unqualified Example 6 Unqualified

[0096] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any other way. Any person skilled in the art may make changes or equivalent modifications to the above-disclosed technical content. However, any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A emamectin benzoate agent for preventing and treating pine wood nematode disease, characterized in that: The drug is an aqueous emulsion. The raw materials for preparing the aqueous emulsion include, by weight percentage, 3 wt% of emamectin benzoate, 25 wt% of a surfactant, 15 wt% of an auxiliary agent, and the balance is a solvent; The solvent is a mixture of methanol and water, and the mass ratio of methanol to water is 1:3; The surfactant is a mixture of polyoxyethylene sorbitan and polyoxyethylene fatty alcohol ether, and the mass ratio of the polyoxyethylene sorbitan to the polyoxyethylene fatty alcohol ether is 1:1; The polyoxyethylene sorbitan is polyoxyethylene sorbitan monooleate, and the HLB value of the polyoxyethylene sorbitan monooleate is 15; The polyoxyethylene fatty alcohol ether is AEO9, and the HLB value of AEO9 is 12.5; The auxiliary agent includes a defoamer, a light stabilizer and a thickener, and the mass ratio of the defoamer, the light stabilizer and the thickener is 10:0.1:0.1; The defoamer is a mixture of diethylene glycol butyl ether and polyether polyol, and the mass ratio of the diethylene glycol butyl ether to the polyether polyol is 2:1; The polyether polyol has a hydroxyl value of 54.5-57.5 mgKOH / g and a number average molecular weight of 3000; The light stabilizer is a mixture of wgwinD902 and butylated hydroxyanisole, and the mass ratio of wgwinD902 to butylated hydroxyanisole is 1:1; The thickener is gum arabic.

2. A method for preparing the emamectin benzoate agent for preventing and controlling pine wood nematode disease according to claim 1, characterized in that: The method at least comprises the steps of: physically mixing emamectin benzoate, a surfactant, an adjuvant and a solvent.

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