A cell culture roller bottle feeding system

Abstract

This application relates to the technical field of cell culture equipment, and more particularly to a cell culture roller bottle feeding system, which includes a horizontal conveying device and a temporary bottle storage device disposed above the conveying device; the temporary bottle storage device includes a turntable and a stop; the turntable is connected to a drive assembly for rotating the turntable; the rotation axis of the turntable is horizontally arranged; the edge of the turntable has multiple U-shaped notches facing away from the center of the turntable; the U-shaped notches are used to store cell culture roller bottles; the stop is circumferentially disposed around the turntable and blocks the openings of the U-shaped notches; the stop has a clearance notch at the lower part of the turntable; the stop has a sterilization notch at the upper part of the turntable; a sterilization device for sterilizing the cell culture roller bottles is disposed at the position of the sterilization notch; a lifting device is disposed below the clearance notch, and the lifting device is located between the conveying device and the temporary bottle storage device. This application has the effect of reducing the labor intensity during the sterilization process before cell culture roller bottle operation.

Description

Technical Field

[0001] This application relates to the technical field of cell culture equipment, and more particularly to a cell culture roller bottle feeding system. Background Technology

[0002] Biological cell culture technology is a primary technique for the large-scale production of biological products such as viral vaccines. In vitro cell culture, primarily using roller bottle culture, is widely applied, especially in vaccine production, such as rabies, Japanese encephalitis, and oral thrush vaccines. During the roller bottle preparation process, various measures are required to prevent microbial contamination, particularly during open-top operations such as adding culture media and discarding waste liquid.

[0003] In related technologies, when adding culture to a cell culture roller bottle, the operator first needs to hold the cell culture roller bottle and wipe its outer surface with a sterilizing solution. Then, the bottle is placed in a Class A laminar flow hood, the roller bottle is opened inside the hood, and the culture is added to it.

[0004] However, the inventors of the above structure believe that the large number of cell culture rollers required for workers to manually wipe and sterilize them results in a high level of labor intensity for the workers. Summary of the Invention

[0005] To reduce the labor intensity of the sterilization process before cell culture roller bottle operation, this application provides a cell culture roller bottle feeding system.

[0006] This application provides a cell culture roller bottle feeding system, which adopts the following technical solution:

[0007] A cell culture roller bottle feeding system includes a horizontal conveying device and a temporary storage device disposed above the conveying device. The temporary storage device includes a turntable and a stop. The turntable is connected to a drive assembly for rotating the turntable. The rotation axis of the turntable is horizontal. The edge of the turntable has multiple U-shaped notches facing away from the center of the turntable. The U-shaped notches are used to store cell culture roller bottles. The stop is circumferentially disposed around the turntable, blocking the openings of the U-shaped notches. The stop has a clearance notch at the lower part of the turntable. The stop has a sterilization notch at the upper part of the turntable. A sterilization device for sterilizing the cell culture roller bottles is disposed at the sterilization notch. A lifting device is disposed below the clearance notch, and the lifting device is located between the conveying device and the temporary storage device.

[0008] By adopting the above technical solution, during use, the horizontal conveying device transports the cell culture flasks directly below the temporary storage device. Simultaneously, the lifting device is at its lowest position and lifts the cell culture flasks, allowing them to enter the U-shaped notch of the turntable. The turntable then rotates to seal the U-shaped notch with a stop. After multiple cell culture flasks are placed on the turntable, the lifting device seals the clearance notch, and the drive assembly drives the turntable to rotate continuously. Meanwhile, the sterilization device sterilizes the side walls of the cell culture flasks from the sterilization notch. During rotation, because the cell culture flasks are located on the turntable... When the lower part of the bottle reaches the stop block, it will abut against the stop block and rotate along the stop block. When it reaches the sterilization notch, the cell culture flask will face the sterilization device at different positions, so that the cell culture flask will achieve a better sterilization effect around its circumference. After sterilization, the cell culture flask will be rotated to the lifting device and placed one by one onto the conveyor device, thus completing the bottle loading. This reduces the labor intensity of the staff during the cell culture flask sterilization process. At the same time, by placing the cell culture flask on the conveyor device, the sterilization of the cell culture flask is automatically completed, reducing the contamination of the cell culture flask by the staff.

[0009] Preferably, a positioning baffle is fixedly provided on the turntable; the positioning baffle is parallel to the turntable and is used to abut against one end of the cell culture flask.

[0010] By adopting the above technical solution, the positioning baffle is fixedly connected to the turntable. When one end of the cell culture flask abuts against the positioning baffle, the positioning baffle positions the cell culture flask and reduces the axial movement of the cell culture flask.

[0011] Preferably, one end of the positioning baffle is provided with a driving device for axially moving the cell culture flask; a return wheel is provided on the side of the turntable away from the positioning baffle for pushing the cell culture flask against the positioning baffle; the return wheel and the driving device are offset from each other and the sterilization device corresponds to the cell culture flask installed between the return wheel and the driving device.

[0012] By adopting the above technical solution, the driving device first pushes the cell culture roller bottle away from the positioning baffle, so that there is a gap between the cell culture roller bottle and the positioning baffle, allowing the sterilization device to sterilize the end of the cell culture roller bottle; then, through the engagement of the return wheel, the cell culture roller bottle is re-engaged on the positioning baffle, ensuring more comprehensive sterilization of the cell culture roller bottle. At the same time, the return wheel can also control the cell culture roller bottle to remain in contact with the positioning baffle, reducing the risk of the cell culture roller bottle falling off due to excessive movement in one direction.

[0013] Preferably, the driving device includes a push rod, a roller, a spring, and a mounting sleeve; one end of the push rod faces the positioning baffle and abuts against the side of the positioning baffle facing away from the turntable; the roller is rotatably connected to the end of the push rod, the spring is sleeved on the push rod, and the push rod is slidably fitted onto the mounting sleeve; one end of the spring abuts against the push rod, and the other end abuts against the mounting sleeve; the force of the spring is used to drive the roller to abut against the positioning baffle; the positioning baffle has a through hole at a position corresponding to the end of the cell culture flask.

[0014] By adopting the above technical solution, a spring is installed on the push rod. Under the action of the spring, the push rod abuts against one end of the positioning baffle. When the through hole on the positioning baffle rotates to the position opposite to the roller, part of the roller enters into the through hole, causing the roller to push the cell culture flask to move axially. Then the positioning baffle continues to rotate, and the roller moves out of the through hole, thus facilitating the control of the movement of the cell culture flask.

[0015] Preferably, the conveying device includes two parallel and spaced-apart conveyor belts; the lifting device is located between the two conveyor belts; and multiple spacer blocks are evenly arranged on the outer side wall of the conveyor belts along the length of the conveyor belts.

[0016] By adopting the above technical solution, there are two conveyor belts, which allows the lifting device to be placed between the two conveyor belts. The cell culture flasks can be transported more stably when placed on the two conveyor belts. At the same time, the spacers on the conveyor belts can reduce the movement of the cell culture flasks on the conveyor belts.

[0017] Preferably, a guide plate is provided on each side of the conveying device; one of the guide plates is located directly below the positioning baffle; an inclined plate is provided on the end of the guide plate near the end of the conveying device; the end of the inclined plate away from the guide plate gradually tilts away from the conveying device.

[0018] By adopting the above technical solution, when the cell culture roller bottle enters the position between the two inclined plates, the inclined plates cause the cell culture roller bottle to move axially, thereby aligning the cell culture roller bottle with the guide plate, which facilitates the positioning of the cell culture roller bottle.

[0019] Preferably, the lifting device includes a linear drive component, a lifting plate, and a support pad; the linear drive component is vertically arranged and the lifting plate is fixed to the upper end of the linear drive component; the support pad is fixed to the upper surface of the lifting plate; the support pad corresponds to the clearance notch; the upper surface of the support pad is a downwardly concave arc shape.

[0020] By adopting the above technical solution, the linear drive can drive the lifting plate to move up and down in the vertical direction when it is working. The support pad on the upper surface of the lifting plate supports the side wall of the cell culture roller bottle, so that the cell culture roller bottle can move between the conveying device and the temporary storage device. At the same time, the upper surface of the support pad is concave arc to reduce the cell culture roller bottle from falling.

[0021] Preferably, arc-shaped rubber blocks are provided on both sides of the stop; the rubber blocks are provided along the edge of the stop; the rubber blocks are located on the side of the stop facing the turntable and are used to abut against the cell culture flask.

[0022] By adopting the above technical solution, the rubber block is arc-shaped and is set along the edge of the stop. When the cell culture flask comes into contact with the rubber block, the friction between the rubber block and the cell culture flask is greater. This ensures that the cell culture flask rotates on the rubber block when the turntable rotates, so that different positions of the cell culture flask face the sterilization device.

[0023] Preferably, a U-shaped strip made of plastic material is fixedly provided on the turntable; the U-shaped strip is arranged along the edge of the U-shaped notch, and the side of the U-shaped strip closest to the inside of the U-shaped notch protrudes from the inner wall of the U-shaped strip.

[0024] By adopting the above technical solution, the U-shaped strip made of plastic material can abut against the side wall of the cell culture roller bottle when the cell culture roller bottle enters the U-shaped notch, reducing wear on the side wall of the cell culture roller bottle.

[0025] Preferably, there are two turntables, which are parallel and spaced apart, and a plurality of support rods of equal length are provided between the two turntables; the turntables are fixed to the ends of the support rods; and the opposite U-shaped notches on the two turntables are used to install a cell culture flask.

[0026] By adopting the above technical solution, two turntables are set, and multiple support rods of equal length are placed between the two turntables so that the length of the support rods positions the turntables, thereby placing the two turntables in a parallel position. This ensures that the U-shaped notches on the two turntables are facing the same direction, guaranteeing the installation of cell culture roller bottles.

[0027] In summary, this application includes at least one of the following beneficial technical effects:

[0028] 1. The U-shaped notch on the turntable can drive multiple cell culture roller bottles to rotate, while the sterilization device sterilizes the cell culture roller bottles, reducing the labor intensity of the staff during the cell culture roller bottle sterilization process and reducing the contamination of the cell culture roller bottles by the staff.

[0029] 2. By moving the cell culture roller bottle horizontally in the axial position, the end of the cell culture roller bottle can also be sterilized when it is horizontally aligned;

[0030] 3. The U-shaped strip made of plastic material can abut against the side wall of the cell culture roller bottle when the cell culture roller bottle enters the U-shaped notch, reducing wear on the side wall of the cell culture roller bottle. Attached Figure Description

[0031] Figure 1 This is a schematic diagram showing the top of the operating compartment cut open according to an embodiment of this application;

[0032] Figure 2 This is a schematic diagram of the overall structure of an embodiment of this application;

[0033] Figure 3 This is a schematic diagram showing the position of the lifting device in an embodiment of this application;

[0034] Figure 4 This is a schematic diagram of the temporary storage bottle device in the embodiments of this application;

[0035] Figure 5 This is a schematic diagram of the structure of the driving device in the embodiments of this application;

[0036] Figure 6 This is a partial structural schematic diagram of the stop block in an embodiment of this application.

[0037] Explanation of reference numerals in the attached drawings: 1. Operating chamber; 11. Chamber door; 2. Cell culture roller; 3. Mounting rack; 4. Conveying device; 41. Conveyor belt; 42. Conveyor wheel; 43. Spacer block; 44. Guide plate; 45. Inclined plate; 5. Temporary bottle storage device; 51. Turntable; 511. U-shaped notch; 52. Stop block; 521. Rubber block; 53. Drive assembly; 531. Drive motor; 532. Reducer; 533. Rotating shaft; 54. Support rod; 55. U-shaped strip; 56. Clearance notch; 57. Sterilization notch; 58. Positioning baffle; 581. Through hole; 59. Bottle storage position; 6. Sterilization device; 7. Placement platform; 8. Lifting device; 81. Linear drive component; 82. Lifting plate; 83. Support pad; 9. Drive device; 91. Top rod; 92. Roller; 93. Spring; 94. Mounting sleeve; 95. Return wheel. Detailed Implementation

[0038] The following is in conjunction with the appendix Figure 1-6 This application will be described in further detail.

[0039] refer to Figure 1This application discloses a cell culture roller bottle feeding system located in an operating chamber 1. The operating chamber 1 has a door 11 on its side wall. The interior of the operating chamber 1 is a sealed space. Opening the door 11 facilitates the insertion of cell culture roller bottles 2 into the operating chamber 1. The operating chamber 1 also contains equipment for operating the cell culture roller bottles 2, such as a robotic arm, a feeder, and a capping machine. The cell culture roller bottles 2 placed in the operating chamber 1 first enter the feeding system and then flow out of it. The equipment within the operating chamber 1 automates the operation of the cell culture roller bottles 2, and then the roller bottles 2 are removed through the door 11. The cell culture roller bottles 2 are sterilized before passing through the feeding system, thereby reducing microbial contamination that may occur when the roller bottles 2 are opened and operated within the operating chamber 1.

[0040] refer to Figure 2 The bottle feeding system includes a mounting rack 3, a conveying device 4, a temporary bottle storage device 5, and a sterilization device 6. The conveying device 4 is horizontally positioned, with a placement platform 7 at one end. The placement platform 7 is located on one side of the mounting rack 3, aligning with the door 11 of the operating chamber 1. Operators can place the cell culture rollers 2 onto the placement platform 7, then move them to one end of the conveying device 4, and then move them to the temporary bottle storage device 5 via the conveying device 4. The temporary bottle storage device 5 is located directly above the conveying device 4 and spaced apart from it. A lifting device 8 is provided on the mounting frame 3. The lifting device 8 is located at the end of the conveying device 4 away from the placement table 7 and is vertically set. When the cell culture roller flask 2 moves to the position of the lifting device 8, the lifting device 8 is directly below the cell culture roller flask 2. By raising the lifting device 8, the cell culture roller flask 2 can move to the position of the temporary storage device 5 under the action of the lifting device 8. The temporary storage device 5 has multiple storage positions 59, which allows the cell culture roller flask 2 to move upward and enter the storage positions 59 for storage. The sterilization device 6 sterilizes the cell culture roller flask 2 located in the storage position 59. After sterilization, the cell culture roller flask 2 is moved from the storage position 59 to the lifting device 8 and placed on the conveying device 4 by the descent of the lifting device 8. The conveying device 4 transfers the cell culture roller flask 2 to the placement table 7 for operation by the equipment that operates the cell culture roller flask 2.

[0041] refer to Figure 2The conveying device 4 includes two parallel conveyor belts 41 spaced apart. Each end of the conveyor belt 41 is provided with a conveyor wheel 42, which supports and drives the conveyor belt 41. The conveyor belt 41 is horizontally positioned. Multiple spacer blocks 43 are uniformly fixed on the outer surface of the conveyor belt 41 along its length. Adjacent spacer blocks 43 are spaced apart and used to block the cell culture flask 2. When the cell culture flask 2 is on the conveying device 4, the cell culture flask 2 is horizontal, and both ends of the cell culture flask 2 are located on the two conveyor belts 41, making the conveying process of the cell culture flask 2 on the conveying device 4 more stable and reducing the movement or tipping of the cell culture flask 2 during the conveying process.

[0042] refer to Figure 2 Two guide plates 44 are fixedly installed on the mounting frame 3. The guide plates 44 are located on both sides of the conveying device 4, and are vertical and parallel to the conveying direction of the conveying device 4. The distance between the two guide plates 44 is equal to the height of the cell culture roller flask 2, so that the two ends of the cell culture roller flask 2 can be positioned under the action of the guide plates 44 during the conveying process on the conveyor belt 41. An inclined plate 45 is provided near the end of the guide plate 44 of the conveying device 4. The inclined plate 45 gradually tilts outward from the guide plate 44 in a direction away from the conveying device 4, so that the two inclined plates 45 gradually move closer to each other from the position away from the guide plate 44 to the position of the guide plate 44, so that the cell culture roller flask 2 entering the conveyor belt 41 can be gradually aligned under the action of the inclined plate 45.

[0043] refer to Figure 2 The lifting device 8 is located between the two conveyor belts 41. The lifting device 8 includes a linear drive 81, a lifting plate 82, and a support pad 83. The linear drive 81 is a hydraulic cylinder and is vertically arranged. The lifting plate 82 is fixed to the upper end of the linear drive 81 and is horizontally arranged. The support pad 83 is fixed to the upper surface of the lifting plate 82, so that the support pad 83 supports the cell culture roller bottle 2. The support pad 83 is made of rubber material, and the surface of the support pad 83 facing away from the lifting plate 82 is set in a downward concave arc shape, so that the cell culture roller bottle 2 is relatively stable on the support pad 83. When the cell culture roller bottle 2 is lifted and lowered by the lifting device 8, the cell culture roller bottle 2 is reduced from falling.

[0044] refer to Figure 3 and Figure 4The temporary storage device 5 includes a turntable 51 and a stop block 52. The turntable 51 is connected to a drive assembly 53 for rotating the turntable 51. The rotation axis of the turntable 51 is horizontally arranged, and two turntables 51 are spaced parallel to each other. Multiple support rods 54 are arranged between the two turntables 51. The two ends of each support rod 54 are fixedly connected to the corresponding turntable 51, either by welding or bolting. The multiple support rods 54 are of equal length, ensuring that the two turntables 51 are in a parallel position when their opposing surfaces abut against the end faces of the support rods 54. The turntable 51 is circular, with multiple U-shaped notches 511 along its edge. Each U-shaped notch 511 serves as a storage bottle position 59. The orientation of the U-shaped notches 511 is radially away from the center of the turntable 51, which is directly above the lifting device 8. A U-shaped strip 55 is positioned on the turntable 51 corresponding to each U-shaped notch 511. The U-shaped strip 55 runs along the edge of the U-shaped notch 511, with the side of the U-shaped strip 55 protruding from the inner wall of the U-shaped notch 511 to allow it to contact the outer wall of the cell culture flask 2. The U-shaped strip 55 is made of wear-resistant plastic to reduce wear on the sidewalls of the cell culture flask 2. When the turntable 51 rotates, the opening of the lowest storage bottle position 59 faces downwards and is directly opposite the lifting device 8, allowing the cell culture flask 2 placed on the lifting device 8 to enter the storage bottle position 59. The stop block 52 is arc-shaped and fixed on the mounting bracket 3. The stop block 52 is arranged around the circumference of the turntable 51 to block the opening of the storage bottle position 59 on the turntable 51. When the storage bottle position 59 on the turntable 51 is in the lower half of the turntable 51, the cell culture flask 2 in the storage bottle position 59 moves towards the opening of the storage bottle position 59, and then the stop block 52 blocks the cell culture flask 2 to prevent the cell culture flask 2 from falling off. At the same time, there is friction between the side wall of the cell culture flask 2 and the stop block 52, so that when the turntable 51 pushes the cell culture flask 2 to rotate, the cell culture flask 2 rolls along the edge of the stop block 52 so that the cell culture flask 2 rotates around its own axis. When the storage bottle position 59 on the turntable 51 is in the upper half of the turntable 51, the cell culture flask 2 in the storage bottle position 59 rolls to the bottom of the storage bottle position 59 under the action of gravity.

[0045] refer to Figure 3A clearance notch 56 is formed at the position where the stop block 52 is directly opposite the lifting device 8, so that the cell culture roller bottle 2 can enter the storage bottle position 59 through the clearance notch 56. The width of the clearance notch 56 is equal to the opening width of the storage bottle position 59 and the width of the support pad 83. When the support pad 83 moves upward, it can enter the clearance notch 56 and block the clearance notch 56, so that the cell culture roller bottle 2 can move across the clearance notch 56 to the position of the stop block 52. In this embodiment, there are 10 storage bottle positions 59 so that more cell culture roller bottles 2 can be sterilized in one sterilization process. A sterilization notch 57 is provided through the stop block 52. The sterilization notch 57 is located at the position corresponding to the upper half of the turntable 51, so that when the storage bottle position 59 rotates to the upper half of the turntable 51 and corresponds to the sterilization notch 57, the cell culture flask 2 can be at the bottom of the storage bottle position 59. The sterilization device 6 is a nozzle, which faces the sterilization notch 57. The nozzle can be long and strip-shaped or multiple nozzles can be arranged along the length of the cell culture flask 2 so that the outer side of the cell culture flask 2 can be sterilized. The atomized liquid sprayed by the nozzle is hydrogen peroxide, which improves the sterilization effect of the cell culture flask 2.

[0046] refer to Figure 3 and Figure 5A positioning baffle 58 is fixedly installed on a turntable 51. The positioning baffle 58 is parallel to the turntable 51, and the side of the positioning baffle 58 facing the turntable 51 is used to abut the end of the cell culture flask 2. The side of the positioning baffle 58 facing the cell culture flask 2 and the guide plate 44 below it are flush with the surface of the cell culture flask 2. A driving device 9 for driving the axial movement of the cell culture flask 2 is provided on the side of the positioning baffle 58 facing away from the turntable 51. The driving device 9 includes a push rod 91, a roller 92, a spring 93, and a mounting sleeve 94. The mounting sleeve 94 is fixed on the mounting frame 3, and the push rod 91 is slidably connected to the mounting sleeve 94. The length direction of the push rod 91 is parallel to the axis of the turntable 51. A through hole 581 is opened on the positioning baffle 58. The diameter of the through hole 581 is smaller than the diameter of the roller 92 and smaller than the end diameter of the cell culture flask 2. The through hole 581 is directly opposite the end of the cell culture flask 2. One end of the push rod 91 faces the positioning baffle 58, and a roller 92 is rotatably connected to the push rod 91. The roller 92 abuts against the side of the positioning baffle 58 facing away from the turntable 51. The distance from the roller 92 to the center of the turntable 51 is equal to the distance from the through hole 581 to the center of the turntable 51. A spring 93 is sleeved on the push rod 91, with one end abutting against the push rod 91 and the other end abutting against the mounting sleeve 94. The force of the spring 93 drives the push rod 91 towards the positioning baffle 58. When the positioning baffle 58 rotates, and the through hole 581 moves to a position directly opposite the roller 92, the force of the spring 93 drives a portion of the roller 92 to fall into the through hole 581. The roller 92 can also move axially on the cell culture flask 2 on the other side of the positioning baffle 58, and then roll out of the through hole 581. A return wheel 95 is provided on the mounting frame 3. The return wheel 95 is mounted on the end of the cell culture flask 2 away from the drive device 9 via a support rod. The return wheel 95 is rotatably connected to the support rod. The rotation direction of the return wheel 95 is tangent to the rotation direction of the cell culture flask 2, so that the return wheel 95 can push the cell culture flask 2 along the axis of the cell culture flask 2. The drive device 9 and the return wheel 95 are offset from each other, and the cell culture flask 2 corresponding to the sterilization device 6 is located between the drive device 9 and the return wheel 95. First, the drive device 9 moves the cell culture flask 2 away from the positioning baffle 58. Then, when passing through the sterilization device 6, the end of the cell culture flask 2 is sterilized. Then, the return wheel 95 abuts against the cell culture flask 2, so that the cell culture flask 2 abuts against the positioning baffle 58 again.

[0047] refer to Figure 4 and Figure 6The drive assembly 53 includes a drive motor 531, a reducer 532, and a rotating shaft 533. The rotating shaft 533 is coaxially fixed on the output shaft of the reducer 532. The drive motor 531 is coaxially fixed on the output shaft of the reducer 532. Two turntables 51 are coaxially fixed on the rotating shaft 533. The reducer 532 is fixed on the mounting bracket 3. The rotating shaft 533 is rotatably connected to the mounting bracket 3. The operation of the drive motor 531 can drive the rotating shaft 533 to rotate, so that the turntables 51 rotate. Both sides of the stop 52 are provided with arc-shaped rubber blocks 521. The rubber blocks 521 are arranged along the edge of the stop 52 and are located on the side of the stop 52 facing the turntable 51. When the cell culture flask 2 located in the storage bottle position 59 comes into contact with the rubber block 521, a large frictional force can be generated between the rubber block 521 and the cell culture flask 2, thereby causing the cell culture flask 2 to rotate under the action of the rubber block 521, so that when the cell culture flask 2 reaches the position of the sterilization device 6, different sides can correspond to the sterilization device 6.

[0048] The working process of this embodiment:

[0049] First, the staff places 10 cell culture roller bottles 2 consecutively onto the conveyor device 4. The conveyor device 4 moves the cell culture roller bottles 2 to the position of the lifting device 8. The lifting device 8 then lifts the cell culture roller bottles 2 one by one to the storage positions 59 on the turntable 51. After each cell culture roller bottle 2 is lifted, the turntable 51 rotates by the angle occupied by one storage position 59. After 10 cell culture roller bottles 2 have been lifted, the support pad 83 in the lifting device 8 keeps the clearance gap 56 formed by the stop block 52 sealed. Then, the staff closes the door 11. At this time, the sterilization device 6 sterilizes the cell culture flask 2. During the sterilization process, the turntable 51 rotates continuously, and the cell culture flask 2 can rotate on its own under the action of the rubber block 521 and the turntable 51, so that the sterilization device 6 sterilizes different positions on the side wall of the cell culture flask 2. After sterilization is completed, the lifting device 8 places the cell culture flask 2 on the storage bottle position 59 one by one downward onto the conveying device 4, and the conveying device 4 completes the feeding process of the cell culture flask 2.

[0050] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cell culture roller bottle feeding system, characterized in that: The device includes a horizontal conveying device (4) and a temporary storage bottle device (5) positioned above the conveying device (4); the temporary storage bottle device (5) includes a turntable (51) and a stop (52); the turntable (51) is connected to a drive assembly (53) for rotating the turntable (51); the rotation axis of the turntable (51) is horizontally positioned; the edge of the turntable (51) has multiple U-shaped notches (511) facing away from the center of the turntable (51); the U-shaped notches (511) are used to store cell culture flasks (2); the stop (52) is circumferentially positioned. A U-shaped notch (511) is positioned around the turntable (51); a clearance notch (56) is formed at the lower part of the turntable (51); a sterilization notch (57) is formed at the upper part of the turntable (51); a sterilization device (6) for sterilizing the cell culture flask (2) is provided at the position of the sterilization notch (57); a lifting device (8) is provided below the clearance notch (56), and the lifting device (8) is located between the conveying device (4) and the temporary storage bottle device (5).

2. The cell culture roller bottle feeding system according to claim 1, characterized in that: A positioning baffle (58) is fixedly provided on the turntable (51); the positioning baffle (58) is parallel to the turntable (51) and is used to abut against one end of the cell culture flask (2).

3. The cell culture roller bottle feeding system according to claim 2, characterized in that: One end of the positioning baffle (58) is provided with a driving device (9) for pushing the cell culture flask (2) to move axially; a return wheel (95) is provided on the side of the turntable (51) away from the positioning baffle (58) for pushing the cell culture flask (2) to abut against the positioning baffle (58); the return wheel (95) and the driving device (9) are offset from each other and the sterilization device (6) corresponds to the cell culture flask (2) installed between the return wheel (95) and the driving device (9).

4. The cell culture roller bottle feeding system according to claim 3, characterized in that: The driving device (9) includes a push rod (91), a roller (92), a spring (93), and a mounting sleeve (94); one end of the push rod (91) faces the positioning baffle (58) and abuts against the side of the positioning baffle (58) facing away from the turntable (51); the roller (92) is rotatably connected to the end of the push rod (91); the spring (93) is sleeved on the push rod (91), and the push rod (91) is slidably fitted on the mounting sleeve (94); one end of the spring (93) abuts against the push rod (91), and the other end abuts against the mounting sleeve (94); the force of the spring (93) is used to drive the roller (92) to abut against the positioning baffle (58); the positioning baffle (58) has a through hole (581) at the position corresponding to the end of the cell culture flask (2).

5. The cell culture roller bottle feeding system according to claim 1, characterized in that: The conveying device (4) includes two parallel and spaced conveyor belts (41); the lifting device (8) is located between the two conveyor belts (41); a plurality of spacer blocks (43) are evenly arranged on the outer side wall of the conveyor belts (41) along the length of the conveyor belts (41).

6. The cell culture roller bottle feeding system according to claim 2, characterized in that: A guide plate (44) is provided on each side of the conveying device (4); one of the guide plates (44) is located directly below the positioning baffle (58); an inclined plate (45) is provided at the end of the guide plate (44) near the end of the conveying device (4); the end of the inclined plate (45) away from the guide plate (44) gradually tilts away from the conveying device (4).

7. The cell culture roller bottle feeding system according to claim 1, characterized in that: The lifting device (8) includes a linear drive (81), a lifting plate (82), and a support pad (83); the linear drive (81) is vertically arranged and the lifting plate (82) is fixed to the upper end of the linear drive (81); the support pad (83) is fixed to the upper surface of the lifting plate (82); the support pad (83) corresponds to the clearance notch (56); the upper surface of the support pad (83) is a downward concave arc shape.

8. The cell culture roller bottle feeding system according to claim 1, characterized in that: Both sides of the stop (52) are provided with arc-shaped rubber blocks (521); the rubber blocks (521) are provided along the edge of the stop (52); the rubber blocks (521) are located on the side of the stop (52) facing the turntable (51) and are used to abut against the cell culture turntable (2).

9. The cell culture roller bottle feeding system according to claim 1, characterized in that: A U-shaped strip (55) made of plastic material is fixedly installed on the turntable (51); the U-shaped strip (55) is arranged along the edge of the U-shaped notch (511), and the side of the U-shaped strip (55) close to the inside of the U-shaped notch (511) protrudes from the inner wall of the U-shaped strip (55).

10. The cell culture roller bottle feeding system according to claim 1, characterized in that: There are two turntables (51), which are parallel and spaced apart. Multiple support rods (54) of equal length are provided between the two turntables (51). The turntables (51) are fixed to the ends of the support rods (54). The opposite U-shaped notches (511) on the two turntables (51) are used to install a cell culture flask (2).

Images