A rapid diagnosis system for benign and malignant tumor tissue in vitro

Abstract

The present invention belongs to the field of medical diagnosis technology, and specifically relates to a rapid diagnosis system for benign and malignant in vitro tumor tissue, which is used to solve the shortcomings of low diagnostic efficiency, easy missed diagnosis or misdiagnosis, and difficulty in realizing intelligent diagnosis in existing intraoperative frozen pathology diagnosis. The rapid diagnosis system for benign and malignant in vitro tumor tissue includes a box, a control computer, and an electric control console, an in vitro tumor tissue placement platform, a light source, a front imaging system, and a hyperspectral imaging system arranged in the box and arranged in sequence; the present invention can obtain two-dimensional spatial image information and one-dimensional spectral information of the diseased tissue sample at one time, has the technical advantage of unified graph and spectrum, upgrades the information of the diseased tissue sample from the traditional single image data information to the hyperspectral graph data information, and can realize the joint diagnosis of morphology and spectral diagnosis, reducing the phenomenon of missed diagnosis or misdiagnosis due to human factors, and improving the accuracy of diagnosis.

Description

Technical Field

[0001] The invention belongs to the technical field of medical diagnosis, and in particular relates to a rapid diagnosis system for benign and malignant tumor tissues in vitro. Background Art

[0002] At present, the diagnosis of benign and malignant tumors during surgery is mainly based on intraoperative frozen pathology diagnosis technology. Intraoperative frozen pathology is a method of rapidly cooling tissue to a certain hardness under low temperature conditions, then cutting it into thin slices and observing it under a microscope to know whether the tumor is benign or malignant. The surgeon makes further decisions based on the frozen pathology diagnosis report. If the frozen pathology report is benign, the operation ends here. If it is malignant, further operations such as extended resection and complete resection are required. At present, the diagnosis of benign and malignant tumors during clinical surgery is mainly based on this method.

[0003] However, there are certain technical drawbacks in intraoperative frozen pathology diagnosis technology. First, intraoperative freezing requires multiple steps such as freezing, slicing, staining, and sealing, which prolongs the diagnosis time to a certain extent. Therefore, it is difficult to achieve the purpose of rapid diagnosis. Secondly, intraoperative pathological diagnosis requires pathologists to have very rich clinical experience, grasp the suspicious points in place, and make a diagnosis quickly in a very short time. This is a huge test for pathologists, and it is inevitable that there will be some incomplete thinking in a short time. In addition, intraoperative diagnosis does not have auxiliary means such as special staining and immunohistochemistry. Some lesions cannot be clearly diagnosed based on image morphological information alone. At the same time, the influence of various factors in the film making process will affect cell morphology. Therefore, missed diagnosis or misdiagnosis is prone to occur. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the existing intraoperative frozen pathology diagnosis, such as low diagnostic efficiency, easy missed diagnosis or misdiagnosis, and difficulty in realizing intelligent diagnosis, and propose an in vitro tumor tissue benign and malignant rapid diagnosis system.

[0005] In order to solve the deficiencies of the above-mentioned prior art, the present invention provides the following technical solutions:

[0006] A rapid diagnosis system for benign and malignant tumor tissue in vitro, which is special in that it includes a box, a control computer, and an electric control console, an in vitro tumor tissue placement platform, a light source, a front imaging system, and a hyperspectral imaging system which are arranged in sequence in the box;

[0007] The ex vivo tumor tissue placement platform is arranged on an electric control table, and a calibration white board is arranged on the ex vivo tumor tissue placement platform;

[0008] The light source includes a plurality of lighting devices evenly distributed in an annular shape, and the ex vivo tumor tissue placement platform is located on the central axis of the light source;

[0009] The front imaging system is a microscope objective lens or a finite-distance imaging objective lens; the hyperspectral imaging system comprises an incident slit, an imaging biconvex lens, a negative meniscus lens, a diffraction grating and a planar array detector assembly arranged in sequence along the optical path; the optical axis of the hyperspectral imaging system and the optical axis of the front imaging system are both perpendicular to the in vitro tumor tissue placement platform; the light emitted by the light source irradiates the in vitro tumor tissue sample to be analyzed placed on the in vitro tumor tissue placement platform, and the light reflected by the in vitro tumor tissue sample to be analyzed passes through the front imaging system, and then passes through the incident slit, the imaging biconvex lens, the negative meniscus lens, and the diffraction grating in sequence, and finally forms an image on the planar array detector assembly;

[0010] The electric control console and the hyperspectral imaging system are both electrically connected to the control computer.

[0011] Furthermore, the front imaging system is a finite distance fully symmetrical imaging objective lens, which includes a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens coaxially arranged in sequence along the optical path.

[0012] Furthermore, the parameters of the first lens and the eighth lens, the second lens and the seventh lens, the third lens and the sixth lens, and the fourth lens and the fifth lens are all the same, thereby reducing the development cost of the front imaging system.

[0013] Furthermore, the thickness of the first lens and the eighth lens is 2.3 mm, the curvature radii of the light incident surface of the first lens and the light exit surface of the first lens are respectively -10.2 mm and -14.76 mm, and the curvature radii of the light incident surface of the eighth lens and the light exit surface of the eighth lens are respectively 14.76 mm and 10.2 mm;

[0014] The thickness of the second lens and the seventh lens is 3.5 mm, the curvature radii of the light incident surface of the second lens and the light exit surface of the second lens are -29.84 mm and -16.5 mm respectively, and the curvature radii of the light incident surface of the seventh lens and the light exit surface of the seventh lens are 16.5 mm and 29.84 mm respectively;

[0015] The thickness of the third lens and the sixth lens is 2 mm, the curvature radii of the light incident surface of the third lens and the light exit surface of the third lens are respectively -170.73 mm and 23.1 mm, and the curvature radii of the light incident surface of the sixth lens and the light exit surface of the sixth lens are respectively -23.1 mm and 170.73 mm;

[0016] The thickness of the fourth lens and the fifth lens is 3 mm, the curvature radii of the light incident surface and the light exit surface of the fourth lens are 23.1 mm and -25.33 mm respectively, and the curvature radii of the light incident surface and the light exit surface of the fifth lens are 25.33 mm and -23.1 mm respectively.

[0017] Furthermore, the imaging biconvex lens has a thickness of 19 mm, and a curvature radius of 126.8 mm and -93.54 mm; the negative meniscus lens has a thickness of 26 mm, and a curvature radius of -85.47 mm and -233.76 mm; and the diffraction grating has a thickness of 4 mm.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The present invention proposes a rapid diagnosis system for benign and malignant in vitro tumor tissue, comprising a housing, a control computer, and an electric control console, an in vitro tumor tissue placement platform, a light source, a front imaging system, and a hyperspectral imaging system, which are arranged in sequence in the housing. The present invention can obtain two-dimensional spatial image information of a diseased tissue sample and one-dimensional spectral information with fingerprint information of the diseased tissue sample at one time, and has the technical advantage of integrating graphs and spectra. The information of the diseased tissue sample is upgraded from traditional single image data information to hyperspectral graph data information, and the combined diagnosis of morphology and spectral diagnosis can be realized, which can improve the accuracy of diagnosis to a certain extent.

[0020] (2) The present invention proposes a rapid diagnosis system for benign and malignant tumor tissue in vitro, which uses hyperspectral imaging technology to simultaneously obtain image information of diseased tissue in different spectral bands. It can quickly obtain a pseudo-color composite image of the diseased tissue without the need for staining. Compared with the traditional method of first staining the diseased tissue and then diagnosing it, it has higher diagnostic efficiency.

[0021] (3) The in vitro rapid diagnosis system for benign and malignant tumor tissue proposed by the present invention does not require multiple steps such as freezing, slicing, staining, and sealing of the diseased tissue removed during surgery. It can directly and quickly perform high-light spectral imaging and analysis on the diseased tissue, greatly shortening the diagnosis time and improving the efficiency of diagnosis.

[0022] (4) The in vitro rapid diagnosis system for benign and malignant tumor tissue proposed by the present invention integrates big data and artificial intelligence automatic identification technology, which can greatly reduce misdiagnosis caused by factors such as the pathologist's personal experience, knowledge structure, and lack of consideration. At the same time, the application of this technology can also reduce the pathologist's nervousness and the pressure to maintain a calm mind to a certain extent.

[0023] (5) The hyperspectral imaging system of the present invention in the rapid diagnosis system of benign and malignant tumor tissue in vitro has the technical advantages of simple optical path structure, miniaturization, and easy installation and adjustment. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic structural diagram of an embodiment of a rapid diagnosis system for benign or malignant tumor tissue in vitro according to the present invention;

[0025] Figure 2 for Figure 1 Schematic diagram of the structure of the in vitro tumor tissue placement platform;

[0026] Figure 3 for Figure 1 A schematic diagram of the structure of the front imaging system;

[0027] Figure 4 for Figure 1 Schematic diagram of the structure of the medium and high spectral imaging system;

[0028] Figure 5 is a point diagram of a front imaging system in an embodiment of the present invention;

[0029] Figure 6 Schematic diagram of the optical transfer function of the front imaging system in an embodiment of the present invention;

[0030] Figure 7 is a point diagram of a hyperspectral imaging system in an embodiment of the present invention;

[0031] Figure 8 Schematic diagram of the optical transfer function of the hyperspectral imaging system at 450nm in an embodiment of the present invention;

[0032] Fig. 9 Schematic diagram of the optical transfer function of the hyperspectral imaging system at 550nm in an embodiment of the present invention;

[0033] Fig.10 Schematic diagram of the optical transfer function of the hyperspectral imaging system at 650nm in an embodiment of the present invention;

[0034] Fig.11 Schematic diagram of the optical transfer function of the hyperspectral imaging system at 750nm in an embodiment of the present invention;

[0035] Fig.12 Schematic diagram of the optical transfer function of the hyperspectral imaging system at 900nm in an embodiment of the present invention;

[0036] Fig.13 The present invention is a flowchart for rapid diagnosis of benign and malignant tumor tissue in vitro.

[0037] The description of the accompanying drawings is as follows: 01-ex vivo tumor tissue sample to be analyzed; 1-box; 2-control computer; 3-electric control console; 4-ex vivo tumor tissue placement platform, 41-calibration whiteboard; 5-light source; 6-front imaging system, 61-first lens, 62-second lens, 63-third lens, 64-fourth lens, 65-fifth lens, 66-sixth lens, 67-seventh lens, 68-eighth lens; 7-hyperspectral imaging system, 71-incident slit, 72-imaging double convex lens, 73-negative meniscus lens, 74-diffraction grating, 75-array detector assembly. DETAILED DESCRIPTION

[0038] The present invention is further described below in conjunction with the accompanying drawings and exemplary embodiments.

[0039] Reference Figure 1 A rapid diagnosis system for benign and malignant tumor tissue in vitro includes a box 1, a control computer 2, and an electric control console 3, an in vitro tumor tissue placement platform 4, a light source 5, a front imaging system 6, and a hyperspectral imaging system 7, which are arranged in sequence in the box 1. The electric control console 3 and the hyperspectral imaging system 7 are both electrically connected to the control computer 2.

[0040] Reference Figure 2 The in vitro tumor tissue placement platform 4 is arranged on the electric control table 3 , and a calibration white plate 41 and an in vitro tumor tissue sample 01 to be analyzed are arranged on the in vitro tumor tissue placement platform 4 .

[0041] The light source 5 includes a plurality of lighting devices evenly distributed in a ring shape on the front imaging system 6 , and the ex vivo tumor tissue placement platform 4 is located on the central axis of the light source 5 .

[0042] Reference Figure 3 , Figure 5 and Figure 6 The front imaging system 6 is a finite-distance fully symmetrical imaging objective lens, which includes a first lens, a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens coaxially arranged in sequence along the optical path. The specific parameters of the front imaging system 6 are as follows: the spectral range is 450mm~900nm, the magnification is 1, the object field of view is 8mm×8mm, the working distance is 46mm, and the numerical aperture is 0.1.

[0043] Among the lenses of the front imaging system 6 , the parameters of the first lens 61 and the eighth lens 68 , the second lens 62 and the seventh lens 67 , the third lens 63 and the sixth lens 66 , and the fourth lens 64 and the fifth lens 65 are all the same, thereby reducing the development cost of the front imaging system 6 .

[0044] The detailed parameters of each lens of the front imaging system 6 are shown in Table 1:

[0045] Table 1

[0046]

[0047] Reference Figure 4 , Figures 7 to 12 The hyperspectral imaging system 7 includes an incident slit 71, an imaging biconvex lens 72, a negative meniscus lens 73, a diffraction grating 74 and a planar array detector assembly 75 which are sequentially arranged along the optical path. The specific parameters of the hyperspectral imaging system 7 are as follows: the spectral range is 450 mm to 900 nm, the magnification is 1, the numerical aperture is 0.1, the image field is 1 / 2″ target surface, and the detector pixel size is 5.5 μm.

[0048] Detailed parameters of the imaging biconvex lens 72, the negative meniscus lens 73, and the diffraction grating 74 in the hyperspectral imaging system 7 are shown in Table 2:

[0049] Table 2

[0050]

[0051] The optical axis of the hyperspectral imaging system 7 and the optical axis of the front imaging system 6 are both perpendicular to the in vitro tumor tissue placement platform 4 .

[0052] The light emitted by the light source 5 irradiates the in vitro tumor tissue sample 01 to be analyzed placed on the in vitro tumor tissue placement platform 4. The light reflected by the in vitro tumor tissue sample 01 to be analyzed passes through the front imaging system 6, and then passes through the incident slit 71, the imaging double convex lens 72, the negative meniscus lens 73, and the diffraction grating 74 in sequence, and finally forms an image on the area array detector assembly 75.

[0053] Reference Fig.13 The process of using the above-mentioned in vitro tumor tissue benign and malignant rapid diagnosis system to perform in vitro tumor tissue benign and malignant rapid diagnosis includes the following steps:

[0054] Step 1: Place the in vitro tumor tissue sample 01 to be analyzed on the in vitro tumor tissue placement platform 4 through a glass slide; drive the electric control table 3 to adjust the in vitro tumor tissue sample 01 placement platform 4 by controlling the computer 2 to ensure that the in vitro tumor tissue sample 01 to be analyzed is located within the field of view of the front imaging system 6;

[0055] Step 2, start the light source 5, the hyperspectral imaging system 7 and the electric control console 3, collect hyperspectral data of the in vitro tumor tissue sample 01 to be analyzed, and store the data in the control computer 2;

[0056] Step 3, controlling the computer 2 to preprocess the hyperspectral data of the in vitro tumor tissue sample 01 to be analyzed, the preprocessing including spatial information preprocessing and spectral information preprocessing; the spatial information preprocessing includes relative radiation calibration, image enhancement, image segmentation, and morphological feature extraction, and the spectral information preprocessing includes spectral filtering, baseline calibration, normalization, feature spectrum extraction, and data dimension reduction;

[0057] Step 4, controlling the computer 2 to convert the hyperspectral data obtained in step 3 into reflectance spectrum data;

[0058] Step 5, the control computer 2 matches and identifies the reflectance spectrum data obtained in step 4 with the spectrum features in the sample database to obtain the analysis result of the in vitro tumor tissue sample 01 to be analyzed;

[0059] The methods used in step 5 include partial least squares judgment analysis method, spectral waveform matching method, spectral angle matching method, least squares support vector machine method, and artificial neural network method.

[0060] The above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit them. For ordinary professional and technical personnel in the field, the specific technical solutions recorded in the aforementioned embodiments can be modified, or some of the technical features therein can be replaced by equivalents, and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions protected by the present invention.

Claims

1. A rapid diagnosis system for benign and malignant tumor tissue in vitro, characterized by: It comprises a box (1), a control computer (2), and an electric control console (3), an ex vivo tumor tissue placement platform (4), a light source (5), a front imaging system (6), and a hyperspectral imaging system (7) which are arranged in sequence in the box (1); The ex vivo tumor tissue placement platform (4) is arranged on the electric control table (3), and a calibration white board (41) is arranged on the ex vivo tumor tissue placement platform (4); The light source (5) comprises a plurality of lighting devices evenly distributed in an annular shape, and the ex vivo tumor tissue placement platform (4) is located on the central axis of the light source (5); The front imaging system (6) is a finite distance fully symmetrical imaging objective lens, comprising a first lens (61), a second lens (62), a third lens (63), a fourth lens (64), a fifth lens (65), a sixth lens (66), a seventh lens (67) and an eighth lens (68) which are coaxially arranged in sequence along the optical path; the parameters of the first lens (61) and the eighth lens (68), the second lens (62) and the seventh lens (67), the third lens (63) and the sixth lens (66), and the fourth lens (64) and the fifth lens (65) are all the same; the first lens (61), the second lens (62), the third lens (63), the sixth lens (66), the seventh lens (67) and the eighth lens (68) are all concave-convex lenses, and the fourth lens (64) and the fifth lens (65) are all biconvex lenses; The hyperspectral imaging system (7) comprises an incident slit (71), an imaging biconvex lens (72), a negative meniscus lens (73), a diffraction grating (74) and a planar array detector assembly (75) which are sequentially arranged along an optical path; the optical axis of the hyperspectral imaging system (7) and the optical axis of the front imaging system (6) are both perpendicular to the in vitro tumor tissue placement platform (4); the light emitted by the light source (5) irradiates the in vitro tumor tissue sample (01) to be analyzed which is placed on the in vitro tumor tissue placement platform (4); the light reflected by the in vitro tumor tissue sample (01) to be analyzed passes through the front imaging system (6), and then sequentially passes through the incident slit (71), the imaging biconvex lens (72), the negative meniscus lens (73), and the diffraction grating (74), and finally forms an image on the planar array detector assembly (75); The electric control console (3) and the hyperspectral imaging system (7) are both electrically connected to the control computer (2).

2. The rapid diagnosis system for benign and malignant tumor tissue in vitro according to claim 1, characterized in that: The thickness of the first lens (61) and the eighth lens (68) is 2.3 mm, the curvature radii of the light incident surface of the first lens (61) and the light exit surface of the first lens (61) are -10.2 mm and -14.76 mm respectively, and the curvature radii of the light incident surface of the eighth lens (68) and the light exit surface of the eighth lens (68) are 14.76 mm and 10.2 mm respectively; The thickness of the second lens (62) and the seventh lens (67) is 3.5 mm, the curvature radii of the light incident surface of the second lens (62) and the light exit surface of the second lens (62) are -29.84 mm and -16.5 mm respectively, and the curvature radii of the light incident surface of the seventh lens (67) and the light exit surface of the seventh lens (67) are 16.5 mm and 29.84 mm respectively; The thickness of the third lens (63) and the sixth lens (66) is 2 mm, the curvature radii of the light incident surface of the third lens (63) and the light exit surface of the third lens (63) are respectively -170.73 mm and 23.1 mm, and the curvature radii of the light incident surface of the sixth lens (66) and the light exit surface of the sixth lens (66) are respectively -23.1 mm and 170.73 mm; The thickness of the fourth lens (64) and the fifth lens (65) is 3 mm, the curvature radii of the light incident surface of the fourth lens (64) and the light exit surface of the fourth lens (64) are 23.1 mm and -25.33 mm respectively, and the curvature radii of the light incident surface of the fifth lens (65) and the light exit surface of the fifth lens (65) are 25.33 mm and -23.1 mm respectively.

3. A rapid diagnosis system for benign and malignant tumor tissue in vitro according to claim 1 or 2, characterized in that: The imaging biconvex lens (72) has a thickness of 19 mm and a curvature radius of 126.8 mm and -93.54 mm; the negative meniscus lens (73) has a thickness of 26 mm and a curvature radius of -85.47 mm and -233.76 mm; and the diffraction grating (74) has a thickness of 4 mm.

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