An optical device for fluorescent seed sorting

Abstract

The application provides an optical device for fluorescent seed sorting, comprising: a curved mirror, a transparent flat plate with an outer surface and an inner surface; the seeds to be sorted slide on the outer surface, and the curved mirror is arranged on one side of the inner surface; the curved mirror reflects the excitation light and the fluorescent light emitted by the seeds, changes the light paths of the excitation light and the fluorescent light, so that the excitation light irradiates the back surface of the seeds, and the fluorescent light emitted by the back surface of the seeds is emitted from the curved mirror. The excitation light irradiates the back surface of the seeds through the focusing effect of the curved mirror, the intensity of the excited fluorescent light is improved, the fluorescent light excited by the back surface of the seeds is emitted into a detection camera through the curved mirror, the intensity of the fluorescent light detected by the weak fluorescent seeds is improved, and the sorting precision of the fluorescent seeds is further improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of seed sorting, in particular to an optical device for fluorescent seed sorting. BACKGROUND

[0002] The existing experiment utilizes the third-generation hybrid rice technology system to create a genetic engineering cytoplasmic male sterile line with complete pollen abortion and stable sterility, and constructs a genetic engineering maintainer line with endosperm fluorescence by using the endosperm fluorescence labeling technology and the transgenic pollen escape prevention technology. The engineering maintainer line can produce genetic engineering cytoplasmic male sterile seeds without transgenic components and fluorescent reproduction line seeds through selfing.

[0003] At present, the sterile line seeds and the reproduction line seeds need to be separated by a sorting device to ensure that the sterile line does not contain the reproduction line seeds. The current sorting instrument adopts the means of exciting the fluorescent protein in the reproduction line seeds by laser to generate fluorescence, and then detects the fluorescence signal by a camera to realize the sorting of fluorescent and non-fluorescent seeds through image processing.

[0004] Since the fluorescent intensity of the fluorescent seeds is different, and the internal fluorescence needs to be emitted through the seed coat due to the shielding of the rice seed coat, the fluorescence intensity is weak, which is not conducive to the efficient detection of the camera and limits the high-precision identification of the fluorescent seeds. SUMMARY

[0005] The present application provides an optical device for fluorescent seed sorting to solve the above problems.

[0006] To achieve the above purpose, the present application adopts the following specific technical solutions:

[0007] An optical device for fluorescent seed sorting, comprising: a curved mirror, a transparent flat plate having an outer surface and an inner surface; the seeds to be sorted slide on the outer surface, and the curved mirror is arranged on one side of the inner surface; the curved mirror reflects the excitation light and the fluorescence emitted by the seeds, changes their light paths, so that the excitation light irradiates the back of the seeds, and the fluorescence emitted by the back of the seeds exits the curved mirror.

[0008] Preferably, the entire edge of the curved mirror is attached to the inner surface.

[0009] Preferably, it further comprises an excitation light source, a detection camera and a focusing lens; the excitation light source emits excitation light for exciting the fluorescence of the seeds; the fluorescence emitted by the seeds passes through the focusing lens and enters the lens of the detection camera, and the detection camera detects whether the seed is a fluorescent seed.

[0010] Preferably, the mirror surface of the curved mirror is a parabolic surface, and the focus of the parabolic surface is located on the outer surface.

[0011] Preferably, a half-transmission half-reflection mirror is further included, the half-transmission half-reflection mirror is arranged between the curved mirror and the focusing lens, and the half-transmission half-reflection mirror reflects the excitation light and transmits the fluorescent light.

[0012] Preferably, the mirror surface of the curved mirror is a curved surface composed of two parabolic surfaces, the two parabolic surfaces have their focal points on the outer surface, and the two focal points coincide.

[0013] Preferably, the two parabolic surfaces are a first parabolic surface and a second parabolic surface, the first parabolic surface reflects the fluorescent light, and the second parabolic surface reflects the excitation light.

[0014] The present application can achieve the following technical effects:

[0015] The focusing effect of the curved mirror enables the excitation light to irradiate the back surface of the seed, improves the intensity of the excited fluorescent light, and enables the fluorescent light excited from the back surface of the seed to enter the detection camera through the curved mirror, improves the intensity of the fluorescent light detected by the weak fluorescent seed, and further improves the sorting precision of the fluorescent seed. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a structural schematic diagram of a parabolic mirror and a transparent flat plate according to Embodiment 1 of the present application;

[0017] Figure 2 is a light path schematic diagram of excitation light of an optical device according to Embodiment 1 of the present application;

[0018] Figure 3 is a light path schematic diagram of fluorescent light of an optical device according to Embodiment 1 of the present application;

[0019] Figure 4 is a structural schematic diagram of a double-parabolic mirror according to Embodiment 2 of the present application;

[0020] Figure 5 is a light path schematic diagram of excitation light of an optical device according to Embodiment 2 of the present application;

[0021] Figure 6 is a light path schematic diagram of fluorescent light of an optical device according to Embodiment 2 of the present application.

[0022] The reference signs in the drawings include: parabolic mirror 1, transparent flat plate 2, excitation light source 3, detection camera 4, focusing lens 5, half-transmission half-reflection mirror 6, excitation light 7, back surface fluorescent light 8, front surface fluorescent light 9, first parabolic surface 10, and second parabolic surface 11. DETAILED DESCRIPTION

[0023] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application is further described in detail below with reference to the drawings and specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, and do not constitute a limitation on the present application.

[0024] As shown in Figures 1-6 , an optical device for fluorescent seed sorting, comprising: a curved mirror, a transparent flat plate 2 having an outer surface and an inner surface; the seeds to be sorted slide on the outer surface, the curved mirror is arranged on one side of the inner surface, the seeds slide on the outer surface of the transparent flat plate 2, so that the seeds can move to the irradiation area of the excitation light 7; the curved mirror reflects the excitation light 7 and the fluorescence emitted by the seeds, changes their light paths, so that the excitation light 7 irradiates to the back surface of the seeds, and the back surface fluorescence 8 emitted by the back surface of the seeds is emitted from the curved mirror, so that the excitation light 7 can irradiate to both the front surface and the back surface of the seeds at the same time, the front surface fluorescence 9 and the back surface fluorescence 8 of the seeds are excited at the same time, and both the front surface fluorescence 9 and the back surface fluorescence 8 can be detected, thereby improving the intensity of the fluorescence that can be detected.

[0025] In an embodiment of the present application, the entire edge of the curved mirror is attached to the inner surface, which protects the mirror surface of the curved mirror and prevents external factors such as dust from polluting the curved mirror.

[0026] In an embodiment of the present application, it further comprises an excitation light source 3, a detection camera 4, and a focusing lens 5; the excitation light source 3 emits excitation light 7 for exciting the fluorescence of the seeds; the fluorescence emitted by the seeds passes through the focusing lens 5 and enters the lens of the detection camera 4, the detection camera 4 detects whether the seed is a fluorescent seed, and the focusing lens 5 converges the fluorescence to prevent the fluorescence from being unable to be detected due to the deviation of the propagation direction from the lens of the detection camera 4.

[0027] As shown in Figure 1 , in embodiment 1 of the present application, the curved mirror is a parabolic mirror 1, and the focus of the parabolic mirror is located on the outer surface of the transparent flat plate 2, which is the detection position for detecting the seeds.

[0028] In embodiment 1 of the present application, it further comprises a half-mirror 6, which is arranged between the parabolic mirror 1 and the focusing lens 5, reflects the excitation light 7, and transmits the back surface fluorescence 8, so that the excitation light 7 and the back surface fluorescence 8 do not interfere with each other.

[0029] The working principle of embodiment 1 will be described in detail below. Figure 2 , 3

[0030] As shown in Figure 2 , when the seeds slide along the transparent flat plate 2 to the focal position of the parabolic mirror 1, the excitation light source 3 emits excitation light 7, the excitation light 7 is reflected on the surface of the half-mirror 6 and is shot to the seeds, part of which irradiates the front surface of the seeds, and part of which passes through the transparent flat plate 2 and irradiates the surface of the parabolic mirror 1, focusing on the focal position, i.e. the back surface of the seeds;

[0031] As​Figure 3 As shown in the figure, the fluorescence of the seed is excited by the excitation light 7, the front surface of the seed generates front surface fluorescence 9, and the back surface of the seed generates back surface fluorescence 8; the back surface fluorescence 8 is approximately light emitted by a point light source at a focal point, is reflected by the parabolic mirror 1 to generate a nearly parallel light beam, is transmitted by the half-transmission half-reflection mirror 6 together with the front surface fluorescence 9, and is collected by the focusing lens 5 to be incident into the detection camera 4.

[0032] As shown in the figure, Figure 4 In the embodiment 2 of the present application, the mirror surface of the curved mirror is a curved surface composed of two parabolic surfaces, the focal points of the two parabolic surfaces are located on the outer surface of the transparent flat plate 2, and the two focal points coincide, and the coincident focal point is the position for detecting the seed.

[0033] In the embodiment 2 of the present application, the two parabolic surfaces are a first parabolic surface 10 and a second parabolic surface 11, the first parabolic surface 10 reflects the back surface fluorescence 8, and the second parabolic surface 11 reflects the excitation light 7, so that the excitation light 7 and the back surface fluorescence 8 do not interfere with each other by being reflected by the two parabolic surfaces respectively.

[0034] The working principle of the embodiment 2 will be described in detail below. Figure 5 , 6 The working principle of the embodiment 2 will be described in detail below.

[0035] As shown in the figure, Figure 5 When the seed slides to the coincident focal point position of the double parabolic mirror along the transparent flat plate 2, the excitation light source 3 emits the excitation light 7, part of the excitation light 7 irradiates the front surface of the seed, and the other part of the excitation light 7 irradiates the surface of the second parabolic surface 11 of the double parabolic mirror through the transparent flat plate 2, and is focused at the focal point, i.e. the back surface of the seed.

[0036] As shown in the figure, Figure 6 The fluorescence of the seed is excited by the excitation light 7, the front surface of the seed generates front surface fluorescence 9, and the back surface of the seed generates back surface fluorescence 8; the back surface fluorescence 8 is approximately light emitted by a point light source at a focal point, is reflected by the first parabolic surface 10 of the double parabolic mirror to generate a nearly parallel light beam, is collected by the focusing lens 5 together with the front surface fluorescence 9, and is incident into the detection camera 4.

[0037] In the description of the specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Also, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction.

[0038] Although the embodiments of the present application have been shown and described above, it is understood that the above-described embodiments are exemplary and are not to be construed as limiting the present application. Those of ordinary skill in the art can make changes, modifications, replacements and variations to the above-described embodiments within the scope of the present application.

[0039] The specific embodiments of the present application described above do not constitute a limitation on the scope of protection of the present application. Any various other corresponding changes and modifications made in accordance with the technical concept of the present application should be included within the scope of protection of the claims of the present application.

Claims

1. An optical arrangement for fluorescent seed sorting, characterized in that, The curved mirror comprises: a transparent flat plate having an outer surface and an inner surface, seeds to be sorted sliding on the outer surface, the curved mirror being arranged on the side of the inner surface; the curved mirror reflecting excitation light and fluorescence emitted by the seeds, changing the optical path of the excitation light and the fluorescence, so that the excitation light irradiates the back surface of the seeds, and the fluorescence emitted by the back surface of the seeds exits the curved mirror; further comprising an excitation light source, a detection camera, and a focusing lens; the excitation light source emits excitation light for exciting fluorescence of the seeds; the fluorescence emitted by the seeds passes through the focusing lens and enters the lens of the detection camera, and the detection camera detects whether the seed is a fluorescent seed; further comprising a half-transmission half-reflection mirror, which is arranged between the curved mirror and the focusing lens, reflects the excitation light, and transmits the fluorescence; when the seeds slide on the transparent flat plate to the focal point position of the curved mirror, the excitation light source emits excitation light, the excitation light is reflected on the surface of the half-transmission half-reflection mirror, and is directed to the seeds, part of which irradiates the front surface of the seeds, and part of which directly passes through the transparent flat plate and irradiates the surface of the curved mirror, and is focused to the focal point, i.e. the back surface of the seeds; the fluorescence of the seeds is excited by the excitation light, the front surface of the seeds generates front surface fluorescence, and the back surface of the seeds generates back surface fluorescence; the back surface fluorescence is reflected by the curved mirror to generate a nearly parallel light beam, and the back surface fluorescence, together with the front surface fluorescence, is directed to the half-transmission half-reflection mirror without passing through the seeds, is transmitted by the half-transmission half-reflection mirror, is converged by the focusing lens, and is incident into the detection camera; the entire edge of the curved mirror is attached to the inner surface.

2. The optical arrangement for fluorescent seed sorting of claim 1, wherein, the mirror surface of the curved mirror is a parabolic surface, and the focal point of the parabolic surface is located on the outer surface.

3. The optical arrangement for fluorescent seed sorting of claim 1, wherein, the mirror surface of the curved mirror is a curved surface composed of two parabolic surfaces, the focal points of the two parabolic surfaces are located on the outer surface, and the two focal points coincide.

4. The optical arrangement for fluorescent seed sorting of claim 3, wherein, the two parabolic surfaces are a first parabolic surface and a second parabolic surface, the first parabolic surface reflects the fluorescence, and the second parabolic surface reflects the excitation light.

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