Platelet-Rich Plasma Preparation Equipment, Method, Device, and Storage Medium

Abstract

The present invention provides a platelet-rich plasma preparation device, method, apparatus, and storage medium, relating to the technical field of blood separation. This platelet-rich plasma preparation method includes: S1, obtaining an image of a centrifuge tube captured by a photographing component; S2, using an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image; S3, constructing a coordinate system based on the image; S4, according to the coordinate system, obtaining the coordinates of the scale value, red blood cell liquid level, and plasma liquid level, and calculating the initial red blood cell volume and the initial plasma volume based on the coordinates; S5, obtaining the counting ratio of the platelet-rich plasma, and determining the plasma discard volume and the red blood cell discard volume based on the counting ratio, the initial red blood cell volume, and the initial plasma volume; S6, controlling the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to respectively extract the red blood cells and serum in the centrifuge tube, in order to obtain platelet-rich plasma with a predetermined counting ratio.

Description

Technical Field

[0001] The present invention relates to the technical field of blood separation, and in particular, to an apparatus, method, device and storage medium for preparing platelet-rich plasma. Background Art

[0002] Platelet-rich plasma (i.e., plasma rich in platelets, also known as: platelet-rich plasma) is usually obtained by centrifuging blood and then extracting the unnecessary components.

[0003] In the prior art, for the preparation of platelet-rich plasma in clinical treatment, the removal of red blood cells and serum is manually extracted by medical staff. The manual operation obtains the volume of plasma and each component by estimation, and it is difficult to accurately determine the volume of removed red blood cells and serum, resulting in too large an error in the remaining plasma volume in the centrifuge tube, and further resulting in inaccurate platelet count ratio, affecting the clinical treatment effect.

[0004] In addition, when manually operating, the extraction speed is not easy to control, and the applied force is uneven, resulting in unstable decline of the plasma liquid level. Seriously, turbulence is generated in the centrifuge tube, resulting in intensified diffusion movement of platelets in the middle layer of the centrifuged plasma, and platelets escaping with the removed red blood cells and serum, and further resulting in inaccurate platelet count ratio and affecting the treatment effect.

[0005] In view of this, the applicant has specifically proposed this application after studying the existing technologies. Summary of the Invention

[0006] The present invention provides an apparatus, method, device and storage medium for preparing platelet-rich plasma to improve at least one of the above technical problems.

[0007] First Aspect,

[0008] An embodiment of the present invention provides an apparatus for preparing platelet-rich plasma, which includes an extraction component, a photographing component and a control component.

[0009] The extraction component includes a fixed bracket, a lead screw rotatably arranged on the fixed bracket, a motor drivingly connected to the lead screw, a slider drivingly connected to the lead screw, a first clamp arranged on the slider, and a second clamp and a third clamp arranged on the fixed bracket; the axis of the lead screw is arranged along the vertical direction; the first clamp, the second clamp and the third clamp are arranged at intervals along the vertical direction; the third clamp is configured to clamp a centrifuge tube; the second clamp is configured to clamp the body of a syringe; the first clamp is configured to clamp the piston of the syringe; the motor can drive the lead screw to rotate to drive the slider to move up and down, so as to drive the syringe to extract the liquid in the centrifuge tube;

[0010] The photographing component is configured to be able to photograph the test tube clamped by the third clamp;

[0011] The control component is electrically connected to the motor and the photographing component, and is used to receive the picture photographed by the photographing component and to control the start and stop of the motor;

[0012] The control component includes a processor, a memory, and a computer program stored in the memory; the computer program can be executed by the processor to execute steps S1 to S6.

[0013] S1. Obtain an image containing a centrifuge tube photographed by the photographing component;

[0014] S2. Use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image;

[0015] S3. Construct a coordinate system according to the image;

[0016] S4. According to the coordinate system, obtain the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level, and calculate the initial red blood cell volume and the initial plasma volume according to the coordinates;

[0017] S5. Obtain the counting ratio of platelet-rich plasma, and determine the plasma removal volume and the red blood cell removal volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume;

[0018] S6. Control the operation of the motor according to the plasma removal volume and the red blood cell removal volume, so as to drive the syringe to extract red blood cells and serum in the centrifuge tube respectively, so as to obtain platelet-rich plasma with a predetermined counting ratio.

[0019] In a second aspect,

[0020] An embodiment of the present invention provides a method for preparing platelet-rich plasma, which includes steps S1 to S6.

[0021] S1. Obtain an image containing a centrifuge tube photographed by the photographing component;

[0022] S2. Use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image;

[0023] S3. Construct a coordinate system according to the image;

[0024] S4. According to the coordinate system, obtain the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level, and calculate the initial red blood cell volume and the initial plasma volume according to the coordinates;

[0025] S5. Obtain the counting ratio of the platelet-rich plasma, and determine the plasma discard volume and the red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume;

[0026] S6. Control the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to extract the red blood cells and serum in the centrifuge tube respectively, in order to obtain the platelet-rich plasma with a predetermined counting ratio.

[0027] In a third aspect,

[0028] An embodiment of the present invention provides a platelet-rich plasma preparation device, which includes:

[0029] An image acquisition module, configured to acquire an image including a centrifuge tube captured by a shooting component;

[0030] An image recognition module, configured to use an image recognition algorithm to recognize the scale value, the scale line, the red blood cell liquid level, and the plasma liquid level in the image;

[0031] A coordinate system construction module, configured to construct a coordinate system according to the image;

[0032] A first volume calculation module, configured to obtain the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level according to the coordinate system, and calculate the initial red blood cell volume and the initial plasma volume according to the coordinates;

[0033] A second volume calculation module, configured to obtain the counting ratio of the platelet-rich plasma, and determine the plasma discard volume and the red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume;

[0034] A discard module, configured to control the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to extract the red blood cells and serum in the centrifuge tube respectively, in order to obtain the platelet-rich plasma with a predetermined counting ratio.

[0035] In a fourth aspect,

[0036] An embodiment of the present invention provides a computer-readable storage medium. The computer-readable storage medium includes a stored computer program, wherein when the computer program runs, it controls the device where the computer-readable storage medium is located to execute the platelet-rich plasma preparation method described in any paragraph of the second aspect.

[0037] By adopting the above technical solutions, the present invention can achieve the following technical effects:

[0038] The platelet-rich plasma preparation device according to the embodiments of the present invention obtains the picture of the centrifuge tube through the photographing component, and automatically extracts each component of the centrifuged plasma through the extraction component, avoiding the errors caused by manual operation. Moreover, it can avoid the situation of escape during the extraction process, resulting in the escape of red blood cells or serum, and further ensures the quality of platelet-rich plasma.

[0039] By intelligently identifying the total volume of plasma and the volume of each component, as well as machine vision component elements such as the liquid level, edge, scale value, scale, etc. of the centrifuge tube and each component of the plasma. Furthermore, according to the preset platelet count ratio, intelligently calculate the volume of red blood cells and serum that need to be removed, distribute control instructions according to the state of the preparation process, and drive the micro-flow negative pressure actuator to operate smoothly and slowly, automatically extracting the red blood cells and serum that need to be discarded. The extraction process is stable and accurate, ensuring low platelet escape and guaranteeing the quality of platelet-rich plasma.

[0040] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following specifically enumerates preferred embodiments and, in conjunction with the accompanying drawings, makes the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0042] Figure 1 is the schematic diagram of the principle of the platelet-rich plasma preparation device.

[0043] Figure 2 is the simplified structural diagram of the platelet-rich plasma preparation device.

[0044] Figure 3 The simplified structural diagram of the fixture.

[0045] Figure 4 is the schematic flow chart of the platelet-rich plasma preparation method.

[0046] Figure 5 is the schematic diagram of the image recognition result.

[0047] Figure 6 is the schematic diagram of the coordinates of the liquid levels of red blood cells and plasma.

[0048] Figure 7 is the schematic diagram of the scale value coordinates.

[0049] Figure 8 is the logic diagram of the platelet-rich plasma preparation method.

[0050] Figure 9 It is a structural schematic diagram of a platelet-rich plasma preparation device.

[0051] Markings in the figure: 1 - motor, 2 - lead screw, 3 - lead screw, 4 - fixed bracket, 5 - centrifuge tube, 6 - third fixture, 7 - second fixture, 8 - tube body, 9 - first fixture, 10 - piston, 11 - first clamping part, 12 - elastic part, 13 - first drive plate, 14 - second clamping part, 15 - second drive part. Detailed implementation manners

[0052] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0053] For a better understanding of the technical solutions of the present invention, the embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0054] The terms used in the embodiments of the present invention are only for the purpose of describing specific embodiments, and are not intended to limit the present invention. The singular forms "a", "the" and "said" used in the embodiments of the present invention and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise.

[0055] It should be understood that the term " / and" used herein is only a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " herein generally represents an "or" relationship between the associated objects before and after.

[0056] Depending on the context, the word "if" as used herein can be interpreted as "when" or "while" or "in response to determining" or "in response to detecting". Similarly, depending on the context, the phrase "if determined" or "if detecting (stated condition or event)" can be interpreted as "when determined" or "in response to determining" or "when detecting (stated condition or event)" or "in response to detecting (stated condition or event)".

[0057] The "first / second" mentioned in the embodiments is only used to distinguish similar objects and does not represent a specific order for the objects. It can be understood that the "first / second" can be interchanged with a specific order or sequence under allowable circumstances. It should be understood that the objects distinguished by the "first / second" can be interchanged under appropriate circumstances so that the embodiments described herein can be implemented in an order other than those illustrated or described herein.

[0058] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments:

[0059] Embodiment 1:

[0060] Please refer to Figures 1 to 3 , the first embodiment of the present invention provides a platelet-rich plasma preparation device, which includes an extraction component, a photographing component, and a control component.

[0061] The extraction component includes a fixed bracket 4, a lead screw 2 rotatably arranged on the fixed bracket 4, a motor 1 drivingly connected to the lead screw 2, a slider 3 drivingly connected to the lead screw 2, a first clamp 9 arranged on the slider 3, and a second clamp 7 and a third clamp 6 arranged on the fixed bracket 4. Preferably, both ends of the lead screw 2 are rotatably arranged on the fixed bracket 4 so that the axis of the lead screw 2 is arranged along the vertical direction. The first clamp 9, the second clamp 7, and the third clamp 6 are arranged at intervals along the vertical direction to fix the centrifuge tube 5 in a vertical state. The photographing component is configured to be able to photograph the test tube clamped by the third clamp 6. The control component is electrically connected to the motor 1 and the photographing component for receiving the picture taken by the photographing component and for controlling the start and stop of the motor 1.

[0062] Specifically, the third clamp 6 is configured to clamp the centrifuge tube 5. The second clamp 7 is configured to clamp the body 8 of the syringe. The first clamp 9 is configured to clamp the piston 10 of the syringe. The motor 1 can drive the lead screw 2 to rotate to drive the slider 3 to move up and down, so as to drive the syringe to extract the liquid in the centrifuge tube 5. Preferably, the motor 1 is an ultrasonic motor 1. In other embodiments, the motor 1 can use existing motors such as a servo motor 1 or a stepper motor 1. Preferably, the slider 3 is arranged on the fixed bracket 4 so as to be movable in the vertical direction. In other embodiments, the slider 3 only needs to be arranged on the lead screw 2 and there is no direct cooperation relationship with the fixed bracket 4.

[0063] The photographing component can be fixedly arranged on the fixed bracket 4 or can be fixed by using an external device such as a tripod to photograph the centrifuge tube 5. The present invention does not limit the specific model and specific fixing method of the photographing component as long as it can photograph the centrifuge tube 5 fixed on the clamp.

[0064] In this embodiment, the control component may include a smart phone, a tablet computer, an industrial computer product, a notebook computer, a desktop computer, etc. In particular, the user terminal is a computer or an industrial computer.

[0065] The platelet-rich plasma preparation device according to the embodiment of the present invention obtains the picture of the centrifuge tube 5 through the photographing component, and automatically extracts each component of the centrifuged plasma through the extraction component, avoiding the error caused by manual operation. And it can avoid the situation of escape during the extraction process, resulting in the escape of red blood cells or serum, further ensuring the quality of platelet-rich plasma.

[0066] As Figure 3 shown, on the basis of the above embodiment, in an optional embodiment of the present invention, at least one of the first clamp 9, the second clamp 7 and the third clamp 6 has: a first clamping member, a second clamping member and an elastic member 12. The first clamping member is provided with a first clamping portion 11 and a first driving portion 13. The second clamping member is provided with a second clamping portion 14 and a second driving portion 15. The first clamping member is hinged to the second clamping member. The elastic member 12 is disposed between the first clamping member and the second clamping member to drive the first clamping portion 11 and the second clamping portion 14 to approach each other without external force. The first driving portion 13 and the second driving portion 15 can be stressed to make the first clamping portion 11 and the second clamping portion 14 move away from each other.

[0067] In this embodiment, the structures of the first clamp 9, the second clamp 7 and the third clamp 6 are the same, all of which are the above-mentioned clamping structures, and the ends of the first clamping portions 11 of the three clamps are all hinged to the fixed bracket 4. By means of hinging, the three clamps can sequentially clamp onto the centrifuge tube 5, the syringe body 8 and the syringe piston 10. The situation of interference during the fixing of the centrifuge tube 5 is avoided. By hinging the clamps to the fixed bracket 4, the integrity of the device can be improved and the loss of parts can be prevented.

[0068] In other embodiments, the clamp can be detachably coupled to the fixed bracket 4, that is, first clamp the clamp on the clamped object (centrifuge tube 5, syringe body 8, syringe piston 10), and then fix the three clamps to the fixed bracket 4 and the slider 3 respectively. The present invention does not limit this, as long as the clamp can fix the object to be clamped to the fixed bracket 4 and the slider 3.

[0069] It can be understood that the structures of the three clamps can be freely combined in different structures. Preferably, at least one of the first clamp 9, the second clamp 7 and the third clamp 6 is hinged to the fixed bracket 4. The present invention does not make specific limitations on this.

[0070] As Figure 3As shown, based on the above embodiments, in an alternative embodiment of the present invention, arc-shaped grooves are provided on the opposite sides of the first clamping portion 11 and the second clamping portion 14. Preferably, the extraction assembly further includes an anti-slip pad disposed in the arc-shaped groove. Specifically, the arc-shaped groove can increase the contact area between the fixture and the clamped object, and the anti-slip pad can increase the friction between the clamped object and the fixture, avoiding accidents caused by relative sliding.

[0071] In this embodiment, through the high-precision lead screw 2 structure, driven by the ultrasonic motor 1, the negative pressure extraction mechanism is driven to move slowly, and the red blood cells and serum in the centrifuge tube 5 are extracted at a low speed and smoothly, realizing high-precision platelet-rich plasma preparation under the condition of low platelet escape. The fixture is composed of an "X"-shaped structure fixture and a connecting end connected by a hinge joint. The connecting end is fixedly connected to the fixed bracket 4 or the screw of the lead screw, so that the clip can rotate horizontally around the connecting end through the hinge joint. The two clamping pieces of the "X"-shaped structure clip are fixed by a rotating shaft, and the torsion spring applies tension to the clamping pieces so that they can tightly hold the clamped object.

[0072] The control component includes a processor, a memory, and a computer program stored in the memory. The computer program can be executed by the processor to implement the "method for preparing platelet-rich plasma". As Figure 4 shown, the "method for preparing platelet-rich plasma" includes steps S1 to S6.

[0073] S1. Obtain an image of the centrifuge tube captured by the imaging component.

[0074] Specifically, the details of the centrifuge tube and the centrifuged plasma are as Figure 5 shown. The centrifuge tube is photographed by a camera (i.e., the imaging component) at a time interval T. Then, in subsequent steps, machine vision technology is used to obtain the position information of the centrifuge tube, the scale on the tube wall, the plasma liquid level, and the red blood cell liquid level.

[0075] After obtaining the image of the centrifuge tube, it needs to be intelligently recognized to calculate the volume of each component in the centrifuged plasma.

[0076] S2. Use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image. In an alternative embodiment, step S2 includes steps S21 and S22.

[0077] S21. Use an image edge detection algorithm to recognize the contour of the centrifuge tube, and separate the target image of the centrifuge tube from the image according to the contour.

[0078] S22. Use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the target image.

[0079] Specifically, for the classification and recognition of centrifuged plasma objects, first, an image edge detection algorithm is used to obtain the overall edge contour of the centrifuge tube and separate the centrifuge tube picture object. Then, artificial intelligence image recognition algorithms such as template matching, edge detection, and K-nearest neighbor are used to recognize the centrifugation scale values, scale lines, liquid level boundaries of red blood cells and plasma, so as to realize the target classification and recognition of the centrifuge tube and centrifuged plasma objects. By extracting the image of the centrifuge tube first, the calculation amount in image recognition can be reduced and the recognition speed can be accelerated.

[0080] It should be noted that the image edge detection algorithm, template matching, edge detection, K-nearest neighbor and other algorithms are all existing algorithms. Based on the technical inspiration of the present invention, those skilled in the art can apply them to this field to recognize the edge of the centrifuge tube and each component of the blood in the centrifuge tube.

[0081] S3. Construct a coordinate system according to the image. In an alternative embodiment, step S3 is specifically:

[0082] According to the image, with the upper left corner of the image as the origin, a coordinate system u-v in pixels is established. Where u is the data row coordinate and v is the data column coordinate.

[0083] Specifically, the position of a certain pixel point P in the pixel coordinates is expressed as (u P , v P ), where u P and v P respectively represent the row number and column number where the current pixel point is located.

[0084] S4. According to the coordinate system, obtain the coordinates of the scale value, the liquid level of red blood cells and the liquid level of plasma, and calculate the initial volume of red blood cells and the initial volume of plasma according to the coordinates. In an alternative embodiment, step S4 includes steps S41 to S43.

[0085] S41. According to the coordinate system, obtain the scale value coordinates, the coordinates at the rows such as the left and right tube walls of the centrifuge tube and the scale value coordinates, the coordinates of the left and right ends of the red blood cell liquid level, and the coordinates of the left and right ends of the plasma liquid level.

[0086] As Figure 6 shown, the left pixel coordinate of the red blood cell liquid level is recorded as (u rl , v rl ), and the right liquid level coordinate is recorded as (u rr , v rr ). The left pixel coordinate of the plasma liquid level is recorded as (u sl , v sl ), and the right liquid level coordinate is recorded as (u sr , v sr ).

[0087] In other embodiments, the pixel coordinates of the erythrocyte liquid surface except for the left and right sides are marked as (u ri , v ri ), i = 1, 2, …, M, where M is the total number of pixel points of the erythrocyte liquid surface except for the pixel points on both sides. The pixel coordinates of the plasma liquid surface except for the left and right sides are marked as (u si , v si ), i = 1, 2, …, N, where N is the total number of pixel points of the plasma liquid surface except for the pixel points on both sides.

[0088] As Figure 7 shown, the upper and lower scales of the liquid surface are characterized by constant horizontal pixel coordinates, that is, the row coordinates of the scale values represent the rows where the scale lines are located. Mark the left end of the right scale of the upper part of the liquid surface (the scale value on the centrifuge tube is two digits, here referring to the position between the two digits) as (u u , v u ), and then mark the pixel coordinates of the left and right sides of the centrifuge tube wall with the same row coordinates as it (that is, the coordinates at the intersection of the row where the scale value is located and the centrifuge tube wall) as (u u , v ul ) and (u u , v ur ). Mark the left end of the right scale of the lower part of the liquid surface (the scale value on the centrifuge tube is two digits, here referring to the position between the two digits) as (u d , v d ), and then mark the pixel coordinates of the left and right sides of the centrifuge tube wall with the same row coordinates as it (that is, the coordinates at the intersection of the row where the scale value is located and the centrifuge tube wall) as (u d , v dl ) and (u d , v dr ).

[0089] S42. Calculate the initial erythrocyte volume according to the coordinates of the left and right ends of the erythrocyte liquid surface. Among them, the erythrocyte volume V r Calculation model:

[0090]

[0091] In the formula, u rl represents the row coordinate of the left end point of the erythrocyte liquid surface. u rr represents the row coordinate of the right end point of the erythrocyte liquid surface. u d represents the row coordinate of the scale value below the erythrocyte liquid surface. v rr represents the column coordinate of the right end point of the erythrocyte liquid surface. v rl represents the column coordinate of the left end point of the erythrocyte liquid surface. v dr represents the column coordinate at the same row as the scale value below the plasma liquid surface on the right side wall of the centrifuge tube. v dlRepresents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube. u u Represents the column coordinate of the scale value above the plasma liquid level. v ur Represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube. v ul Represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube. C r Represents the difference between the scale value above the red blood cell liquid level and the scale value below. l rd Represents the scale value below the red blood cell liquid level. Red blood cell volume V r The unit of

[0092] S43. Calculate the initial total plasma volume based on the coordinates at both ends of the plasma liquid level. Among them, the total plasma volume V b The calculation model is:

[0093]

[0094] In the formula, u sll Represents the row coordinate of the left end point of the plasma liquid level. u sr Represents the row coordinate of the right end point of the plasma liquid level. u d Represents the row coordinate of the scale value below the plasma liquid level. v sr Represents the column coordinate of the right end point of the plasma liquid level. v sl Represents the column coordinate of the left end point of the plasma liquid level. v dr Represents the column coordinate at the same row as the scale value below the plasma liquid level on the right tube wall of the centrifuge tube. v dl Represents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube. u u Represents the row coordinate of the scale value above the plasma liquid level. v ur Represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube. v ul Represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube. C b Represents the difference between the scale value above the plasma liquid level and the scale value below. l bd Represents the scale value below the plasma liquid level. Total plasma volume V b The unit of

[0095] S5. Obtain the counting ratio of platelet-rich plasma, and determine the plasma discard volume and red blood cell discard volume based on the counting ratio, initial red blood cell volume, and initial plasma volume.

[0096] Specifically, the system data processing flow is as Figure 8As shown, after determining the volumes of red blood cells and serum to be discarded according to the counting ratio, the total plasma volume and the volumes of each component are scanned in real time and dynamically, and negative pressure extraction control instructions are distributed as needed, so as to obtain platelet-rich plasma that meets the requirements of the counting ratio.

[0097] On the basis of the above embodiments, in an optional embodiment of the present invention, step S5 includes steps S51 to S54.

[0098] S51. Obtain the counting ratio λ of the platelet-rich plasma.

[0099] S52. Calculate the plasma discard volume according to the counting ratio. Among them, the calculation model of the plasma discard volume v q is:

[0100]

[0101] In the formula, v b represents the total plasma volume, and λ represents the plasma discard volume.

[0102] S53. Calculate the red blood cell discard volume according to the plasma discard volume. Among them, the calculation model of the red blood cell discard volume v qr is:

[0103]

[0104] In the formula, max(a, b) represents taking the larger value of the two values in the brackets, v q represents the plasma discard volume, v b represents the total plasma volume, v r represents the volume of red blood cells in the initial state.

[0105] S54. Calculate the serum discard volume according to the plasma discard volume and the red blood cell discard volume. Among them, the calculation model of the serum discard volume v qs is:

[0106] v qs = v q - v qr

[0107] In the formula, v q represents the plasma discard volume, v qr red blood cell discard volume.

[0108] S6. Control the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to extract red blood cells and serum in the centrifuge tube respectively, so as to obtain platelet-rich plasma with a predetermined counting ratio.

[0109] Specifically, the redundant red blood cells and serum in the preparation process are extracted in two steps. After the first centrifugation of the plasma, the lower-layer red blood cells are extracted, and after the second centrifugation, the upper-layer serum is extracted.

[0110] Based on the above embodiments, in an optional embodiment of the present invention, step S6 includes steps S61 to S64.

[0111] S61. Control the operation of the motor, take pictures of the centrifuge tube at time intervals T, and simultaneously identify the real-time volume of red blood cells.

[0112] Specifically, according to the control instruction distributed by the control component, a pulse code modulation wave is generated to drive the motor. The motor shaft is connected to a high-precision lead screw, which converts the rotational motion of the motor into a linear motion to stably drive the micro-flow negative pressure actuator to operate.

[0113] S62. Calculate the real-time discarded volume of red blood cells according to the initial volume of red blood cells and the real-time volume of red blood cells. When the real-time discarded volume of red blood cells reaches the discarded volume of red blood cells, stop driving the motor.

[0114] Specifically, the operation steps for extracting the lower-layer red blood cells are as follows:

[0115] (I) Fix the negative pressure syringe on the second fixture.

[0116] (II) Apply force to the two clamping parts of the first fixture, open the clip and rotate the first fixture to clamp the extraction piston of the negative pressure syringe.

[0117] (III) First, connect the lower extraction port of the negative pressure syringe to the middle thin tube of the centrifuge tube (as shown in Figure 1 and Figure 2 ), and the lower end of the middle thin tube is located in the red blood cell liquid. Then, hold the centrifuge tube with one hand, pinch the driving part of the third fixture with the other hand and rotate it to clamp the centrifuge tube. When clamping the centrifuge tube, pay attention to making the scale face face the camera so as to collect the plasma volume signal. It should be noted that multiple thin tubes (which can be understood as needles) are installed on the centrifuge tube, and they are fixed on the rubber plug at the tube orifice of the centrifuge tube. By manually adjusting the insertion depth, the liquid level position where the end is located can be adjusted.

[0118] After the centrifuge tube and the negative pressure extraction device (i.e., the syringe) are fixed, the control component dynamically and automatically discriminates the plasma volume in real time and calculates according to the preset platelet counting ratio, and distributes a control instruction to the motor. Drive the motor to operate, and extract the lower-layer red blood cells in the centrifuge tube into the syringe.

[0119] A closed-loop intelligent control extraction is formed among the shooting component, the control component and the extraction component, and the red blood cells in the centrifuge tube are slowly transferred to the syringe. When the red blood cell extraction amount is reached, it will automatically stop. The data during the extraction process is saved in the data storage subsystem.

[0120] S63. After re-centrifuging the centrifuge tube and adjusting the syringe to the position for serum extraction, control the motor to run again, take pictures of the centrifuge tube at time interval T, and simultaneously identify the real-time volume of plasma.

[0121] S64. Calculate the real-time discarded volume of serum based on the initial red blood cell volume, initial plasma volume, discarded red blood cell volume, and the real-time volume of plasma. When the real-time discarded volume of serum reaches the serum discarded volume, stop driving the motor to obtain platelet-rich plasma with a predetermined count ratio.

[0122] The second extraction of the upper-layer serum is as follows:

[0123] (I) Fix the negative-pressure syringe on the second fixture.

[0124] (II) Open the first fixture and rotate it to clamp the extraction piston of the negative-pressure syringe.

[0125] (III) First, connect the lower extraction port of the negative-pressure syringe to the left thin tube of the centrifuge tube (as shown in Figure 1 and Figure 2 , the lower end of the left thin tube is located in the serum liquid), and visually adjust the position of the lower end of the left thin tube to meet the serum extraction requirements according to the volume of serum to be extracted displayed by the interactive system. Then hold the centrifuge tube with one hand and pinch the driving part of the third fixture and rotate it to clamp the centrifuge tube. When clamping the centrifuge tube, pay attention to making the scale face face the camera to collect the plasma volume signal.

[0126] After the centrifuge tube and the negative-pressure extraction device are fixed, the control component calculates data according to the count ratio, drives the motor to run, and extracts the upper-layer serum of the centrifuge tube into the syringe.

[0127] A closed-loop intelligent control extraction is formed among the shooting component, the control component, and the extraction component, and the upper-layer serum of the centrifuge tube is slowly transferred to the syringe, and it will automatically stop after reaching the serum extraction volume. After two extractions, the excess red blood cells and serum are discarded according to the platelet count ratio, so as to obtain a platelet-rich plasma product that meets the treatment requirements.

[0128] By intelligently identifying the total volume of plasma and the volume of each component, as well as machine vision component elements such as the liquid surface, edge, scale value, and scale of the centrifuge tube and each component of the plasma. Furthermore, according to the preset platelet count ratio, intelligently calculate the volume of red blood cells and serum that need to be removed, distribute control instructions according to the state of the preparation process, drive the micro-flow negative-pressure actuator to run smoothly and slowly, and automatically extract the red blood cells and serum to be discarded. The extraction process is stable and accurate, ensuring low platelet escape and guaranteeing the quality of platelet-rich plasma.

[0129] The embodiments of the present invention have the following advantages:

[0130] 1. It can intelligently and real - time identify the volumes of plasma and red blood cells in a centrifuge tube, intelligently calculate the volumes of red blood cells and serum to be discarded according to a preset counting ratio, automatically drive the motor to drive a stable extraction, and obtain platelet - rich plasma with a high - precision counting ratio for clinical treatment in the case of low platelet escape. The above process automatically forms a closed - loop control structure, imitating the manual extraction operation of medical staff, with the advantages of intelligence, precision, and automation, solving common problems such as large workload, large platelet escape, and low precision in manual extraction and separation of blood components in clinical treatment.

[0131] 2. It can intelligently identify the volumes of multi - component solutions in tubes with uneven scales. Through processes such as object target classification and recognition, intelligent recognition of scale values, and calibration of pixel coordinates of each element, the volume of blood components in a centrifuge tube with uneven scales is determined by applying the principle of equal ratio. It has the characteristics of intelligence, speed, and high precision, solving problems such as inconvenient reading and inaccurate measurement during the operation of medical staff, and providing precision measurement support for the preparation of platelet - rich plasma with a high - precision counting ratio.

[0132] 3. Extraction of plasma components with low platelet escape. Through the motor, syringe, control components, and imaging components, the liquid level change is monitored in real - time and corresponding control signals are distributed to control the motor to drive the piston of the syringe to move slowly and smoothly. When extracting, the plasma liquid level drops smoothly without generating turbulence, so that the platelets after centrifugal stratification have a low escape amount, ensuring the platelet content in the remaining plasma and ensuring the accuracy of the counting ratio.

[0133] 4. The fixture consists of an "X" - shaped clip, a hinge joint, and a connection end. The clip rotates around the hinge joint on the horizontal plane, enabling the fixture to face the fixed clamping object and achieving the goal of multi - point clamping with coaxial collinearity. This fixture is convenient for clamping a negative - pressure extraction device and a centrifuge tube in the case of single - person operation, enabling the negative - pressure actuator to extract excess red blood cells and serum according to the counting ratio under the action of the control system.

[0134] Embodiment 2

[0135] The embodiment of the present invention provides a platelet - rich plasma preparation device, which includes:

[0136] An image acquisition module 201, configured to acquire an image containing a centrifuge tube captured by the imaging component.

[0137] An image recognition module 202, configured to use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image.

[0138] A coordinate system construction module 203, configured to construct a coordinate system according to the image.

[0139] A first volume calculation module 204, configured to obtain the coordinates of the scale value, red blood cell liquid level, and plasma liquid level according to the coordinate system, and calculate the initial red blood cell volume and initial plasma volume according to the coordinates.

[0140] A second volume calculation module 205, configured to obtain a count ratio of platelet-rich plasma, and determine a plasma discard volume and a red blood cell discard volume according to the count ratio, an initial red blood cell volume, and an initial plasma volume.

[0141] A discard module 206, configured to control the operation of a motor according to the plasma discard volume and the red blood cell discard volume, so as to drive a syringe to respectively extract red blood cells and serum in a centrifuge tube, so as to obtain platelet-rich plasma with a predetermined count ratio.

[0142] Based on the above embodiments, in an optional embodiment of the present invention, the image recognition module 202 includes:

[0143] A first recognition unit, configured to use an image edge detection algorithm to recognize the contour of the centrifuge tube, and separate a target picture of the centrifuge tube from the image according to the contour.

[0144] A second recognition unit, configured to use an image recognition algorithm to recognize scale values, scale lines, the red blood cell liquid level, and the plasma liquid level in the target picture.

[0145] Based on the above embodiments, in an optional embodiment of the present invention, the coordinate system construction module 203 is specifically configured to:

[0146] According to the image, establish a coordinate system u-v in pixels with the upper left corner of the image as the origin. Where u is the data row coordinate and v is the data column coordinate.

[0147] Based on the above embodiments, in an optional embodiment of the present invention, the first volume calculation module 204 includes:

[0148] A coordinate acquisition unit, configured to acquire scale value coordinates, coordinates at the same row as the left and right tube walls of the centrifuge tube and the scale value coordinates, coordinates of the left and right ends of the red blood cell liquid level, and coordinates of the left and right ends of the plasma liquid level according to the coordinate system.

[0149] A red blood cell volume calculation unit, configured to calculate an initial red blood cell volume according to the coordinates of the left and right ends of the red blood cell liquid level. Wherein, the red blood cell volume V r Calculation model:

[0150]

[0151] In the formula, u rl represents the row coordinate of the left end point of the red blood cell liquid level. u rr represents the row coordinate of the right end point of the red blood cell liquid level. u d represents the row coordinate of the scale value below the red blood cell liquid level. v rr represents the column coordinate of the right end point of the red blood cell liquid level. v rlRepresents the column coordinate of the left endpoint of the red blood cell liquid level. v dr Represents the column coordinate at the same row as the scale value below the plasma liquid level on the right tube wall of the centrifuge tube. v dl Represents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube. u u Represents the column coordinate of the scale value above the plasma liquid level. v ur Represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube. v ul Represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube. C r Represents the difference between the scale value above the red blood cell liquid level and the scale value below. l rd Represents the scale value below the red blood cell liquid level. Red blood cell volume V r The unit of is milliliters.

[0152] Plasma volume calculation unit, used to calculate the initial total plasma volume according to the coordinates of the left and right ends of the plasma liquid level. Among them, the total plasma volume V b The calculation model is:

[0153]

[0154] In the formula, u sl Represents the row coordinate of the left endpoint of the plasma liquid level. u sr Represents the row coordinate of the right endpoint of the plasma liquid level. u d Represents the row coordinate of the scale value below the plasma liquid level. v sr Represents the column coordinate of the right endpoint of the plasma liquid level. v sl Represents the column coordinate of the left endpoint of the plasma liquid level. v dr Represents the column coordinate at the same row as the scale value below the plasma liquid level on the right tube wall of the centrifuge tube. v dl Represents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube. u u Represents the row coordinate of the scale value above the plasma liquid level. v ur Represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube. v ul Represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube. C b Represents the difference between the scale value above the plasma liquid level and the scale value below. l bd Represents the scale value below the plasma liquid level. Total plasma volume V b The unit of is milliliters.

[0155] On the basis of the above embodiments, in an optional embodiment of the present invention, the second volume calculation module 205 includes:

[0156] A counting ratio acquisition unit for acquiring the counting ratio λ of platelet-rich plasma.

[0157] A first discarded volume calculation unit for calculating the plasma discarded volume according to the counting ratio. Wherein, the calculation model of the plasma discarded volume v q is:

[0158]

[0159] In the formula, v b represents the total plasma volume, and λ represents the plasma discarded volume.

[0160] A second discarded volume calculation unit for calculating the red blood cell discarded volume according to the plasma discarded volume.

[0161] Wherein, the calculation model of the red blood cell discarded volume v qr is:

[0162]

[0163] In the formula, max(a, b) represents taking the larger value of the two values in the brackets, v q represents the plasma discarded volume, v b represents the total plasma volume, v r represents the red blood cell volume in the initial state.

[0164] A third discarded volume calculation unit for calculating the serum discarded volume according to the plasma discarded volume and the plasma discarded volume. Wherein, the calculation model of the serum discarded volume v qs is:

[0165] v qs = v q - v qr

[0166] In the formula, v q represents the plasma discarded volume, v qr red blood cell discarded volume.

[0167] Based on the above embodiments, in an optional embodiment of the present invention, the discard module 206 includes:

[0168] A first driving unit for controlling the operation of the motor, taking pictures of the centrifuge tube at time intervals T, and simultaneously identifying the real-time volume of red blood cells.

[0169] A first judgment unit for calculating the real-time discarded volume of red blood cells according to the initial red blood cell volume and the real-time volume of red blood cells, and stopping driving the motor when the real-time discarded volume of red blood cells reaches the red blood cell discarded volume.

[0170] A second driving unit, configured to control the motor to operate again after the centrifuge tube is re-centrifuged and the syringe is adjusted to the position for serum extraction, and capture images of the centrifuge tube at a time interval T, while identifying the real-time volume of plasma.

[0171] A second judgment unit, configured to calculate the real-time discarded volume of serum according to the initial red blood cell volume, the initial plasma volume, the discarded volume of red blood cells, and the real-time volume of plasma. When the real-time discarded volume of serum reaches the discarded volume of serum, stop driving the motor to obtain platelet-rich plasma with a predetermined counting ratio.

[0172] Embodiment 3

[0173] An embodiment of the present invention provides a computer-readable storage medium. The computer-readable storage medium includes a stored computer program, wherein when the computer program runs, it controls the device where the computer-readable storage medium is located to execute the platelet-rich plasma preparation method as described in Embodiment 1.

[0174] In several embodiments provided by the embodiments of the present invention, it should be understood that the disclosed devices and methods can also be implemented in other ways. The device and method embodiments described above are merely illustrative. For example, the flowcharts and block diagrams in the accompanying drawings show the possible architectures, functions, and operations of devices, methods, and computer program products according to multiple embodiments of the present invention. In this regard, each block in the flowchart or block diagram may represent a module, a program segment, or a part of code, and the module, program segment, or part of code contains one or more executable instructions for implementing the specified logical function. It should also be noted that in some alternative implementations, the functions marked in the blocks may occur in a different order than marked in the accompanying drawings. For example, two consecutive blocks may actually be executed substantially in parallel, and they may sometimes be executed in the reverse order, depending on the functions involved. It should also be noted that each block in the block diagram and / or flowchart, and the combination of blocks in the block diagram and / or flowchart, can be implemented by a dedicated hardware-based system for performing the specified functions or actions, or can be implemented by a combination of dedicated hardware and computer instructions.

[0175] In addition, the functional modules in each embodiment of the present invention can be integrated together to form an independent part, or each module can exist alone, or two or more modules can be integrated to form an independent part.

[0176] When the above-mentioned functions are implemented in the form of software function modules and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present invention, in essence, or the part that contributes to the prior art, or a part of this technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, an electronic device, or a network device, etc.) to execute all or part of the steps of the methods described in various embodiments of the present invention. The aforementioned storage medium includes: various media that can store program codes, such as USB flash drives, mobile hard disks, read-only memories (ROM, Read-Only Memory), random access memories (RAM, Random Access Memory), magnetic disks, or optical discs. It should be noted that in this article, the term "including", "comprising", or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article, or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or further includes elements inherent to such a process, method, article, or device. Without further limitations, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article, or device including the said element.

[0177] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A platelet-rich plasma preparation device, characterized in that, Comprising: An extraction component, including a fixed bracket (4), a lead screw (2) rotatably arranged on the fixed bracket (4), a motor (1) drivingly connected to the lead screw (2), a slider (3) drivingly connected to the lead screw (2), a first clamp (9) arranged on the slider (3), and a second clamp (7) and a third clamp (6) arranged on the fixed bracket (4); the axis of the lead screw (2) is arranged along the vertical direction; the first clamp (9), the second clamp (7) and the third clamp (6) are arranged at intervals along the vertical direction; the third clamp (6) is configured to clamp a centrifuge tube (5); the second clamp (7) is configured to clamp the body (8) of a syringe; the first clamp (9) is configured to clamp the piston (10) of the syringe; the motor (1) can drive the lead screw (2) to rotate to drive the slider (3) to move up and down, so as to drive the syringe to extract the liquid in the centrifuge tube (5); A photographing component configured to be able to photograph the test tube clamped by the third clamp (6); A control component electrically connected to the motor (1) and the photographing component, for receiving the picture photographed by the photographing component and for controlling the start and stop of the motor (1); The control component includes a processor, a memory, and a computer program stored in the memory; the computer program can be executed by the processor to perform the following steps: Obtain an image containing the centrifuge tube (5) photographed by the photographing component; Adopt an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level and plasma liquid level in the image; Construct a coordinate system according to the image; According to the coordinate system, obtain the coordinates of the scale value, the red blood cell liquid level and the plasma liquid level, and calculate the initial red blood cell volume and the initial plasma volume according to the coordinates; Obtain the counting ratio of platelet-rich plasma, and determine the plasma removal volume and the red blood cell removal volume according to the counting ratio, the initial red blood cell volume and the initial plasma volume; Control the operation of the motor (1) according to the plasma removal volume and the red blood cell removal volume, so as to drive the syringe to extract red blood cells and serum in the centrifuge tube (5) respectively to obtain platelet-rich plasma with a predetermined counting ratio; Obtain the counting ratio of platelet-rich plasma, and determine the plasma removal volume and the red blood cell removal volume according to the counting ratio, the initial red blood cell volume and the initial plasma volume, specifically including: Obtain the counting ratio of platelet-rich plasma ; Calculate the plasma discard volume according to the counting ratio; wherein, the plasma discard volume has a calculation model of: ; in the formula, represents the total plasma volume, represents the plasma discard volume; Calculate the red blood cell removal volume according to the plasma removal volume; wherein, the red blood cell removal volume The calculation model is: ; In the formula, represents taking the larger value of the two values in the brackets, represents the plasma removal volume, represents the total plasma volume, represents the red blood cell volume in the initial state; Calculate the serum discard volume based on the plasma discard volume and the plasma discard volume; wherein, the serum discard volume has a calculation model of: ; in the formula, represents the plasma discard volume, the red blood cell discard volume.

2. The platelet-rich plasma preparation device according to claim 1, wherein At least one of the first clamp (9), the second clamp (7) and the third clamp (6) is hinged to the fixed bracket (4).

3. The platelet-rich plasma preparation device according to claim 1, characterized in that At least one of the first clamp (9), the second clamp (7) and the third clamp (6) has: a first clamping member, a second clamping member and an elastic member (12); the first clamping member is provided with a first clamping portion (11) and a first driving portion (13); the second clamping member is provided with a second clamping portion (14) and a second driving portion (15); The first clamping member is hinged to the second clamping member; the elastic member (12) is disposed between the first clamping member and the second clamping member to drive the first clamping portion (11) and the second clamping portion (14) to approach each other without external force; the first driving portion (13) and the second driving portion (15) can be stressed to make the first clamping portion (11) and the second clamping portion (14) move away from each other; The end of the first clamping portion (11) is hinged to the fixed bracket (4); Arc-shaped grooves are provided on the opposite sides of the first clamping portion (11) and the second clamping portion (14); The motor (1) is an ultrasonic motor (1).

4. The platelet-rich plasma preparation device according to claim 3, characterized in that, Using an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image, specifically including: Using an image edge detection algorithm to recognize the contour of the centrifuge tube and separating the target picture of the centrifuge tube from the image according to the contour; Using an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the target picture; 5. The platelet-rich plasma preparation device according to claim 3, characterized in that, Constructing a coordinate system according to the image, specifically including: Based on the said image, taking the upper left corner of the image as the origin, a coordinate system in pixels is established. ; where is the data row coordinate, and the data is the column coordinate. According to the coordinate system, obtaining the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level, and calculating the initial red blood cell volume and the initial plasma volume according to the coordinates, specifically including: According to the coordinate system, obtaining the scale value coordinates, the coordinates of the left and right tube walls of the centrifuge tube at the same row as the scale value coordinates, the coordinates of the left and right ends of the red blood cell liquid level, and the coordinates of the left and right ends of the plasma liquid level; Calculate the initial red blood cell volume based on the coordinates of the left and right ends of the red blood cell liquid level; wherein, the red blood cell volume Calculation model: ； In the formula, represents the row coordinate of the left endpoint of the red blood cell liquid level; represents the row coordinate of the right endpoint of the red blood cell liquid level; represents the row coordinate of the scale value below the red blood cell liquid level; represents the column coordinate of the right endpoint of the red blood cell liquid level; represents the column coordinate of the left endpoint of the red blood cell liquid level; represents the column coordinate at the same row as the scale value below the plasma liquid level on the right tube wall of the centrifuge tube; represents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube; represents the column coordinate of the scale value above the plasma liquid level; represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube; represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube; represents the difference between the scale value above the red blood cell liquid level and the scale value below; represents the scale value below the red blood cell liquid level; the red blood cell volume The unit is milliliters. Calculate the initial total plasma volume based on the coordinates of the left and right ends of the plasma liquid surface; wherein, the calculation model of the total plasma volume is: ； In the formula, represents the row coordinate of the left endpoint of the plasma liquid level; represents the row coordinate of the right endpoint of the plasma liquid level; represents the row coordinate of the scale value below the plasma liquid level; represents the column coordinate of the right endpoint of the plasma liquid level; represents the column coordinate of the left endpoint of the plasma liquid level; represents the column coordinate at the same row as the scale value below the plasma liquid level on the right tube wall of the centrifuge tube; represents the column coordinate at the same row as the scale value below the plasma liquid level on the left tube wall of the centrifuge tube; represents the row coordinate of the scale value above the plasma liquid level; represents the column coordinate at the same row as the scale value above the plasma liquid level on the right tube wall of the centrifuge tube; represents the column coordinate at the same row as the scale value above the plasma liquid level on the left tube wall of the centrifuge tube; represents the difference between the scale value above the plasma liquid level and the scale value below it; represents the scale value below the plasma liquid level; the total plasma volume The unit of which is milliliter.

6. The platelet-rich plasma preparation device according to claim 1, characterized in that Controlling the operation of the motor according to the plasma discard volume and the red blood cell discard volume, thereby driving the syringe to extract red blood cells and serum from the centrifuge tube respectively to obtain platelet-rich plasma with a predetermined counting ratio, specifically including: Controlling the operation of the motor and taking pictures of the centrifuge tube at a time interval T, and simultaneously recognizing the real-time volume of red blood cells; Calculating the real-time discard volume of red blood cells according to the initial red blood cell volume and the real-time volume of red blood cells, and stopping driving the motor when the real-time discard volume of red blood cells reaches the red blood cell discard volume; After the centrifuge tube is re-centrifuged and the syringe is adjusted to the position for extracting serum, controlling the motor to operate again, taking pictures of the centrifuge tube at a time interval T, and simultaneously recognizing the real-time volume of plasma; Calculating the real-time discard volume of serum according to the initial red blood cell volume, the initial plasma volume, the red blood cell discard volume, and the real-time volume of plasma; when the real-time discard volume of serum reaches the serum discard volume, stopping driving the motor to obtain platelet-rich plasma with a predetermined counting ratio.

7. A method for preparing platelet-rich plasma, characterized in that, Including: Obtaining an image containing a centrifuge tube captured by the imaging component; Using an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image; Constructing a coordinate system according to the image; According to the coordinate system, obtaining the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level, and calculating the initial red blood cell volume and the initial plasma volume according to the coordinates; Obtain the counting ratio of platelet-rich plasma, and determine the plasma discard volume and red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume; Control the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to draw red blood cells and serum in the centrifuge tube respectively, in order to obtain platelet-rich plasma with a predetermined counting ratio; Obtain the counting ratio of platelet-rich plasma, and determine the plasma discard volume and red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume, specifically including: Obtain the counting ratio of platelet-rich plasma ; Calculate the plasma discard volume according to the counting ratio; wherein, the plasma discard volume has a calculation model of: ; in the formula, represents the total plasma volume, represents the plasma discard volume; Calculate the red blood cell removal volume according to the plasma removal volume; wherein, the red blood cell removal volume The calculation model is: ; In the formula, represents taking the larger value of the two values in the parentheses, represents the plasma removal volume, represents the total plasma volume, represents the red blood cell volume in the initial state; Calculate the serum discard volume based on the plasma discard volume and the plasma discard volume; wherein, the serum discard volume has a calculation model of: ; in the formula, represents the plasma discard volume, the red blood cell discard volume.

8. A platelet-rich plasma preparation device, characterized in that, Comprising: An image acquisition module, configured to acquire an image of a centrifuge tube captured by a photographing component; An image recognition module, configured to use an image recognition algorithm to recognize the scale value, scale line, red blood cell liquid level, and plasma liquid level in the image; A coordinate system construction module, configured to construct a coordinate system according to the image; A first volume calculation module, configured to obtain the coordinates of the scale value, the red blood cell liquid level, and the plasma liquid level according to the coordinate system, and calculate the initial red blood cell volume and the initial plasma volume according to the coordinates; A second volume calculation module, configured to obtain the counting ratio of platelet-rich plasma, and determine the plasma discard volume and red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume; A discard module, configured to control the operation of the motor according to the plasma discard volume and the red blood cell discard volume, so as to drive the syringe to draw red blood cells and serum in the centrifuge tube respectively, in order to obtain platelet-rich plasma with a predetermined counting ratio; Obtain the counting ratio of platelet-rich plasma, and determine the plasma discard volume and red blood cell discard volume according to the counting ratio, the initial red blood cell volume, and the initial plasma volume, specifically including: Obtain the counting ratio of platelet-rich plasma ; Calculate the plasma discard volume according to the counting ratio; wherein, the plasma discard volume has a calculation model of: ; in the formula, represents the total plasma volume, represents the plasma discard volume; Calculate the red blood cell discard volume according to the plasma discard volume; wherein, the red blood cell discard volume The calculation model is: ; In the formula, represents taking the larger value of the two values in the brackets, represents the plasma discard volume, represents the total plasma volume, represents the red blood cell volume in the initial state; Calculate the serum discard volume based on the plasma discard volume and the plasma discard volume; wherein, the serum discard volume has a calculation model of: ; in the formula, represents the plasma discard volume, the red blood cell discard volume.

9. A computer-readable storage medium, characterized in that, The computer-readable storage medium includes a stored computer program, wherein, when the computer program runs, it controls the device where the computer-readable storage medium is located to execute the method for preparing platelet-rich plasma according to claim 7.

Images