A kind of library construction instrument debugging tool and method

Abstract

The present invention relates to a debugging tool and method for a library construction instrument, comprising a driving displacement module, at least one debugging hole and at least one debugging module for outputting a measurement value. The assembled library construction instrument is automatically debugged by means of the debugging tool whose appearance is close to that of an experimental card box, and a standardized debugging process is established, thereby significantly improving the debugging efficiency and accuracy of the library construction instrument, thereby being able to simply improve the debugging precision and efficiency, and in particular being able to assist a control program in accurately calibrating a zero point, thereby improving the accuracy and success rate of a library construction experiment.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a library building instrument debugging tool and method. Background Art

[0002] The library construction instrument is used to automatically complete the library construction and data analysis of NGS. In order to improve the accuracy of the library construction results and reduce the cost of the library construction experiment, the existing technology mostly uses an independent sample cartridge. The library construction experiment needs to connect the cartridge to the library construction instrument and drive the movable parts in the cartridge to work through the library construction instrument. Since the library construction experiment needs to ensure very high operating accuracy, it is necessary to set the zero point of the displacement of the connected cartridge so that the control program can correctly drive the movable parts in the cartridge. The zero point can usually be determined by triggering a photoelectric switch. However, due to assembly errors, the position of the photoelectric switch may be biased, resulting in a deviation in the zero point setting of the movement of the movable parts (such as the tip holder) in the cartridge, which in turn affects the experimental accuracy. Therefore, the zero point setting of the library construction instrument directly determines the success rate and accuracy of the library construction experiment. The debugging of the library construction instrument in the existing technology basically relies on the control of the assembly process, and mainly relies on the visual judgment of the technicians. It cannot solve the debugging needs of the library construction instrument after assembly, resulting in the library construction instrument being prone to zero point deviation and reduced experimental accuracy after long-term use. Summary of the invention

[0003] In order to address the deficiencies in the prior art, the present invention proposes a library construction instrument debugging tool and method, which can automatically debug the assembled library construction instrument through a debugging tool whose appearance is close to that of an experimental card box, thereby easily improving the debugging accuracy and efficiency, especially assisting the control program to accurately calibrate the zero point, establish a standardized debugging process, and significantly improve the debugging efficiency and accuracy of the library construction instrument, thereby improving the accuracy and success rate of the library construction experiment.

[0004] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0005] A debugging tool for a library construction instrument, characterized in that it comprises a driving displacement module, at least one debugging hole and at least one debugging module for outputting a measurement value;

[0006] The driving displacement module includes at least one rotating connecting shaft connected to the driving shaft of the library construction instrument and transmitting torque, and a simulated suction head seat component driven by the rotating connecting shaft to perform displacement;

[0007] The debugging hole position is a horizontal debugging position corresponding to the simulated suction head seat assembly for simulated operation and a simulated card box hole position with a known vertical debugging depth;

[0008] The debugging module includes at least one set of trigger baffles corresponding to the photoelectric switch or the proximity switch, and also includes a measuring device for measuring, recording and outputting numerical values ​​through the trigger control of the photoelectric switch or the proximity switch.

[0009] Furthermore, the simulated suction head seat assembly is driven by the rotating connecting shaft to perform horizontal linear displacement and / or vertical linear displacement.

[0010] Further, the measuring device includes a linear measuring device, or the measuring device includes a linear measuring device and a rotation angle measuring device.

[0011] Furthermore, the linear measuring device is connected to the simulated tip holder assembly and measures, records and outputs the linear displacement value of the simulated tip holder assembly, and the rotation angle measuring device is connected to the library building instrument drive shaft and measures, records and outputs the rotation angle value of the library building instrument drive shaft.

[0012] Furthermore, the driving displacement module includes a first rotating connecting shaft and a second rotating connecting shaft; the first rotating connecting shaft drives the simulated tip holder assembly to perform horizontal left-right displacement by forward and reverse rotation, and the second rotating connecting shaft drives the simulated tip probe in the simulated tip holder assembly to perform vertical up-down displacement by forward and reverse rotation;

[0013] The debugging module includes a first trigger baffle, a first measuring device and a second trigger baffle corresponding to the first photoelectric switch and the second photoelectric switch respectively; the first trigger baffle is fixedly arranged on the simulated suction head holder assembly, and the first measuring device is a linear measuring device; the second trigger baffle is fixedly arranged on the library construction instrument driving shaft connected to and driving the second rotating connecting shaft.

[0014] Furthermore, the debugging module also includes a second measuring device corresponding to the second photoelectric switch, and the second measuring device is a rotation angle measuring device.

[0015] Furthermore, the linear measurement device includes a capacitive scale, a grating scale, a magnetic scale or a laser displacement sensor; and the rotation angle measurement device includes a magnetic angle sensor.

[0016] Furthermore, the horizontal debugging position of the debugging hole corresponding to the simulated suction head seat assembly for the simulated operation is the side projection vertical line distance from the first photoelectric switch trigger position to the center line of the debugging hole;

[0017] A contact detection end is provided at the center of the inner bottom surface of the debugging hole, and the vertical debugging position of the debugging hole corresponding to the simulated suction head seat assembly for simulated operation is the vertical distance between the simulated suction head probe and the contact detection end when the simulated suction head probe moves vertically upward to the limit position.

[0018] Furthermore, the tooling also includes a shell;

[0019] The driving displacement module, the debugging hole and the simulated suction head seat assembly are arranged in the housing;

[0020] The shell size and the opening position are compatible with the library construction instrument card box.

[0021] The present invention also relates to a library building instrument debugging method, which is characterized by using the above-mentioned tooling to debug the library building instrument drive shaft to rotate and drive the displacement distance and / or displacement zero point position of the suction head holder assembly in the library building instrument cartridge.

[0022] Furthermore, the method as described above comprises:

[0023] Insert the library construction instrument debugging tool into the library construction instrument and connect the library construction instrument drive shaft and the tool rotation connection shaft;

[0024] According to the library construction instrument control program, the simulated suction head holder assembly is driven to respectively perform the simulated operations of the horizontal debugging position and the vertical debugging depth of the corresponding debugging hole position;

[0025] Calculate assembly errors by simulating the displacement distance actually performed by the tip holder assembly output by the measuring device;

[0026] Use assembly error to adjust the library construction instrument control program settings;

[0027] According to the adjusted library construction instrument control program settings, the simulation operation is performed again and the new assembly error is recalculated until the assembly error is less than or equal to the preset allowable value.

[0028] Furthermore, the use of assembly error to adjust the library building instrument control program setting includes adjusting the zero point position of the displacement of the tip holder assembly in the library building instrument cartridge, and / or adjusting the correspondence between the rotation angle of the library building instrument drive shaft and the displacement distance of the tip holder assembly in the library building instrument cartridge.

[0029] The beneficial effects of the present invention are:

[0030] The use of the library construction instrument card box debugging tooling and method described in the present invention can realize automated and standardized debugging of the library construction instrument's driven active parts (such as the suction head assembly) in the horizontal and vertical directions. In particular, by using the debugging tooling described in the present invention to set the zero point required during the operation of the library construction instrument, the control parameters and the actual displacement position and distance of the active parts form an accurate correspondence, thereby improving the uncertainty of manual debugging, greatly improving the debugging efficiency and accuracy of the library construction instrument, and being particularly helpful to improve the assembly quality control of the library construction instrument and ensure the accuracy of experiments involving the library construction instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a three-dimensional schematic diagram of the first embodiment of the card box debugging tooling of the library building instrument of the present invention.

[0032] Figure 2This is a perspective schematic diagram of the first embodiment of the card box debugging tooling of the library building instrument of the present invention.

[0033] Figure 3 It is a cross-sectional schematic diagram of the first embodiment of the card box debugging tooling of the library building instrument of the present invention.

[0034] Figure 4 It is a top view schematic diagram of the first embodiment of the library building instrument card box debugging tooling of the present invention (inserted into the library building instrument).

[0035] Figure 5 FIG. 4 is a schematic diagram of a simulated tip holder assembly according to the first embodiment of the present invention.

[0036] Figure 6 This is a three-dimensional schematic diagram of a conventional library construction instrument card box.

[0037] Figure 7 This is a cross-sectional schematic diagram of a conventional library construction instrument card box.

[0038] Figure 8 It is a top view schematic diagram of a conventional library construction instrument card box (inserted into the library construction instrument).

[0039] Fig. 9 It is a schematic side view of a conventional library construction instrument card box.

[0040] Fig.10 This is a schematic diagram of the second embodiment of the card box debugging tooling of the library building instrument of the present invention.

[0041] Fig.11 This is a schematic diagram of the relative positions of the library building instrument card box debugging tool and the library building instrument card box of the present invention.

[0042] Fig.12 It is a three-dimensional schematic diagram of a partially hidden housing according to the first embodiment of the present invention.

[0043] Fig.13 It is a cross-sectional schematic diagram of the initial position of the pressing plate according to the first embodiment of the present invention.

[0044] Fig.14 It is a cross-sectional schematic diagram of the pressing process of the pressing plate in the first embodiment of the present invention.

[0045] Fig.15 FIG. 1 is a schematic diagram of a simulated tip probe conduction cross section according to the first embodiment of the present invention.

[0046] Explanation of the accompanying figures: 11-first rotating connecting shaft, 12-second rotating connecting shaft, 121-pressure plate, 122-reset spring, 13-simulated tip holder assembly, 131-simulated tip probe, 2-debugging hole, 21-contact detection end, 31-first photoelectric switch, 32-first trigger baffle, 33-first measuring device, 331-grating electronic ruler, 34-second photoelectric switch, 35-second trigger baffle, 361-magnetic angle sensor, 37-third photoelectric switch, 4-housing, 51-tip holder, 511-tip, 52-reagent hole. DETAILED DESCRIPTION

[0047] In order to more clearly understand the content of the present invention, it will be described in detail with reference to the drawings and embodiments.

[0048] like Figures 1 to 4 as well as Fig.12 The figure shows the preferred first embodiment of the card box debugging tooling of the library building instrument of the present invention, which can be decomposed into a driving displacement module, a debugging hole 2, a debugging module, and an external covering shell 4 according to the execution division of labor. Figure 1 The external structure of the first embodiment is shown. The main components visible through the hollowed-out portion of the housing 4 include a first rotating connecting shaft 11 in the form of a screw, a second rotating connecting shaft 12, a pressure plate 121 fixedly arranged on the second rotating connecting shaft 12, and a simulated suction head seat assembly 13 mounted on the first rotating connecting shaft 11. By driving the first rotating connecting shaft 11 to rotate, the simulated suction head seat assembly 13 can be driven to move horizontally along a straight line. In terms of the overall external structure, especially on the side used for docking with the library construction instrument, the debugging tool described in the present invention has a size and shape compatible with the actual application card box, so that the debugging tool can be fixedly connected to the library construction instrument in the same installation and limiting manner as the card box. For example, a common push rod structure can be used to ensure that the installation side of the debugging tool is in close contact with the library construction instrument, and at the same time, the first rotating connecting shaft 11 and the second rotating connecting shaft 12 are accurately connected to the corresponding drive shaft.

[0049] like Figure 2 The figure is a perspective view of the interior of the first embodiment with a portion of the housing 4 hidden at another angle. Figure 3The figure shows a cross-sectional schematic diagram of the first embodiment. By driving the second rotating connecting shaft 12, the pressing plate 121 can be driven to rotate and squeeze the simulated tip probe 131 (including the vertical motion component with the jacket structure) on the simulated tip holder assembly 13, so that the simulated tip probe 131 moves vertically downward; when the pressing plate 121 rotates back to its original position, the return spring 122 in the compressed state will push the simulated tip probe 131 to move vertically upward until it is reset. The length of the pressing plate 121 matches the range of the horizontal displacement of the simulated tip holder assembly 13, so that when the simulated tip holder assembly 13 moves horizontally to any specific position, the pressing plate 121 can execute the vertical downward displacement of the simulated tip probe 131. In order to collect the displacement data required for debugging, the first embodiment is also provided with a first measuring device 33 for measuring the linear horizontal displacement. The first measuring device 33 preferably uses a capacitive scale, which can measure the accurate horizontal displacement distance of the simulated suction head seat assembly 13 driven by the first rotating connecting shaft 11; at the same time, a contact detection end 21 is provided inside the debugging hole 2, and the simulated suction head probe 131 directly touches the contact detection end 21 during the vertical downward displacement process to send a signal in place, which is fed back as the number of pulses of the driving motor connected to the second rotating connecting shaft 12, that is, the accurate vertical displacement distance generated by the second rotating connecting shaft 12 driving the pressing plate 121 to rotate and squeeze the simulated suction head probe 131. It can be seen that the rotation amount of the first rotating connecting shaft 11 can be directly related to the horizontal displacement distance of the simulated suction head seat assembly 13, and the rotation amount of the second rotating connecting shaft 12 can be directly related to the vertical displacement distance of the simulated suction head probe 131, and the obtained data can be used for purposes such as correcting operating parameters. According to needs, the debugging hole 2 can be set to multiple different depth specifications, so that the debugging tooling can correspond to the debugging work of reagent holes of different specifications in the cartridge.

[0050] In the first embodiment, the horizontal displacement of the simulated suction head seat assembly 13 and the vertical displacement of the simulated suction head probe 131 are respectively operated by the first photoelectric switch 31 and the second photoelectric switch 34 installed on the library building instrument. Among them, the first photoelectric switch 31 includes a light-emitting part and a light-receiving part respectively arranged on both sides of the debugging tool. The control of the first photoelectric switch 31 can be realized by the linear displacement of the first trigger baffle 32 arranged on the simulated suction head seat assembly 13 (or directly using the linear displacement of the end face of the simulated suction head seat assembly 13); the light-emitting and light-receiving parts of the second photoelectric switch 34 are integrated into one, and the control of the second photoelectric switch 34 can be realized by the rotation of the second trigger baffle 35. The above-mentioned first photoelectric switch 31 and the second photoelectric switch 34 are also used in the actual operation process of the card box in the library building instrument, so the debugging tool can completely simulate the working state of the card box to debug the library building instrument. Preferably, the first embodiment further includes a third photoelectric switch 37, which is fixedly disposed in the wall of the housing 4 and is controlled by the first trigger baffle 32, and can be used to determine the absolute zero point during the debugging process (simulating the tip holder assembly 13 moving to the top position).

[0051] like Figure 5 FIG. 1 is a schematic diagram of the simulated tip holder assembly 13 in the first embodiment. The first trigger block 32 is disposed at one end of the simulated tip holder assembly 13 and moves horizontally along with the simulated tip holder assembly 13. Meanwhile, the simulated tip probe 131 can move vertically according to the operation instruction.

[0052] Figures 6 to 9 A conventional library construction instrument cartridge structure corresponding to the first embodiment is given. It can be seen that some components of the first embodiment are very similar to or completely consistent with the cartridge ( Figures 6 to 9 Some of the components in the middle are numbered the same as those in the first embodiment, indicating that their structure and function are basically the same as those in the debugging tooling of the first embodiment), so that the first embodiment can well simulate the working state of the cartridge to debug the library building instrument; the remaining different parts include the tip holder 51 (corresponding to the simulated tip holder assembly 13), the tip 511 (corresponding to the simulated tip probe 131) and the reagent hole 52 (corresponding to the debugging hole 2). In particular, for the X-axis, B-axis and Z-axis driven by the B-axis that produce displacement in the cartridge, the debugging tooling of the present invention has corresponding ones, and complete debugging can be performed. Compared with conventional cartridges, the debugging tooling of the first embodiment can be understood as a special cartridge. By adding special components such as the first measuring device 33, the simulated tip probe 131 and the contact detection end 21, accurate collection and feedback of displacement data can be achieved, thereby performing debugging work.

[0053] A major source of error in the use of the cartridge is the assembly error of the first photoelectric switch 31 and the second photoelectric switch 34, which leads to inaccurate displacement zero point setting, thereby affecting displacement accuracy. The debugging tool of the present invention can effectively meet this debugging demand. A typical debugging method for horizontal displacement under the structure of the first embodiment includes the following steps:

[0054] After the library instrument is assembled, there is a deviation between the theoretical trigger position and the actual trigger position due to installation errors;

[0055] refer to Fig.11 The relative position relationship between the debugging tool and the cartridge shown in the figure is that the distance between a debugging hole position 2 marked as H' on the debugging tool and the trigger position Y' of the third photoelectric switch 37 on the debugging tool is h', and h' is a known quantity; the corresponding reagent hole 52 on the cartridge and the trigger position of the first photoelectric switch 31 are H and Y respectively, and the distance between H and Y is h', and the trigger position of the third photoelectric switch 37 is set as the reference zero point, and the trigger position of the first photoelectric switch 31 is set as the absolute zero point;

[0056] Insert the debugging tool into the library building instrument, and the motor drives the first rotating connecting shaft 11 to drive the simulated suction head seat assembly 13 to move to the right (such as Figure 3 The position shown, moving away from the debugging hole 2 toward the third photoelectric switch 37);

[0057] The debugging tool is provided with a capacitive scale (first measuring device 33), including a reading head, which can record the actual displacement distance traveled by the simulated suction head seat assembly 13 under the drive of the first rotating connecting shaft 11;

[0058] When the first trigger baffle 32 on the simulated suction head seat assembly 13 moves to the trigger position Y' of the third photoelectric switch 37, the system reads the reading of the capacitive scale and sets the reference zero point of the simulated suction head seat assembly 13 moving along the X-axis direction;

[0059] The motor drives the first rotating connecting shaft 11 to drive the simulated suction head seat assembly 13 to continue to move to the right (such as Figure 3 The position shown, moving away from the debugging hole 2 toward the first photoelectric switch 31);

[0060] When the end surface of the simulated suction head seat assembly 13 (or the first trigger baffle 32, set according to actual needs) moves to the trigger position of the first photoelectric switch 31, the system reads the capacitive scale reading, sets the absolute zero point of the simulated suction head seat assembly 13 moving along the X-axis direction, and records the distance between the absolute zero point and the reference zero point as s, which is determined by the assembly error;

[0061] Take out the debugging tool and insert the card box;

[0062] The motor drives the suction head seat 51 on the card box to move left or right. When the end surface on the card box suction head seat 51 moves to the actual trigger position (i.e., the absolute zero point) of the first photoelectric switch 31, the software resets the motor steps to zero;

[0063] The distance between the reagent hole 52 at a certain mark H on the cartridge and the absolute zero point is h'+s; when it is necessary to move to the reagent hole 52 for pipetting, the system drives the motor to drive the first rotating connecting shaft 11 to drive the tip holder to move a distance of h'+s.

[0064] Similarly, the second photoelectric switch 34 is used to adjust the zero point of the pressing plate 121 along the B axis, and the process may include:

[0065] like Fig.13 As shown, due to the elastic force of the return spring 122, the pressing plate 121 is located at the highest limit position when in the initial position;

[0066] After the debugging tool is inserted into the library building instrument, the motor drives the second rotating connecting shaft 12 and the pressing plate 121 to rotate, forming a Fig.14 Changes shown;

[0067] When the second triggering baffle 35 triggers the second photoelectric switch 34 (in the case of Fig.14 During the movement of the pressing plate 121 shown in the figure, the rotation zero point of the B axis is set. Since the length of the pressing plate 121 matches the range of the horizontal displacement of the simulated tip holder assembly 13, it can be considered that the B axis has the same zero point along the X-axis direction of the horizontal displacement of the simulated tip holder assembly 13;

[0068] The motor drives the first rotating connecting shaft 11 to drive the simulated suction head seat assembly 13 to move along the X-axis direction;

[0069] When the simulated suction head seat assembly 13 moves a distance of h'+s, the motor drives the pressing plate 121 to rotate and squeeze the simulated suction head probe 131 to move vertically downward; when the end of the simulated suction head probe 131 contacts the contact detection end 21 in the debugging hole 2 (such as Fig.15 As shown, since the debugging tool is grounded, the end of the simulated pipette probe 131 will be connected to the debugging tool. At this time, the system records the number of pulses of the drive motor, which corresponds to the depth of the debugging hole position 2 (i.e., the corresponding reagent hole 52);

[0070] The other reagent holes 52 are converted into the rotation angle of the pressing plate 121 according to the hole depth, thereby completing the debugging of the library construction instrument.

[0071] like Fig.10The figure shows a schematic diagram of the second embodiment of the debugging tool for the card box of the library building instrument of the present invention. Different from the first embodiment, the second embodiment uses a grating electronic ruler 331 to measure the horizontal displacement and a magnetic angle sensor 361 to measure the rotation angle of the pressure plate 121 (the rotation angle of the pressure plate 121 can directly correspond to the vertical displacement of the simulated suction head probe 131), which can also achieve a debugging effect similar to that of the first embodiment. The change of the specific components used in the measurement part will not change the content of the debugging tool data output and the debugging results, but because the grating electronic ruler 331 and the magnetic angle sensor 361 or any other applicable sensor may require additional volume to accommodate, the overall volume of the debugging tool is relatively large, which is different from the size of the card box, and there are certain requirements for the applicable library building instrument.

[0072] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A library construction instrument debugging tool, characterized in that: It includes a driving displacement module, at least one debugging hole and at least one debugging module for outputting a measurement value; The driving displacement module includes at least one rotating connecting shaft connected to the driving shaft of the library construction instrument and transmitting torque, and a simulated suction head seat component driven by the rotating connecting shaft to perform displacement; The debugging hole position is a horizontal debugging position corresponding to the simulated suction head seat assembly for simulated operation and a simulated card box hole position with a known vertical debugging depth; The debugging module includes at least one set of trigger baffles corresponding to the photoelectric switch or the proximity switch, and also includes a measuring device for measuring, recording and outputting numerical values ​​through the trigger control of the photoelectric switch or the proximity switch; The simulated suction head seat assembly is driven by the rotating connecting shaft to perform horizontal linear displacement and / or vertical linear displacement; The measuring device includes a linear measuring device, or the measuring device includes a linear measuring device and a rotation angle measuring device; The linear measurement device is connected to the simulated suction head seat assembly and measures, records and outputs the linear displacement value of the simulated suction head seat assembly, and the rotation angle measurement device is connected to the library construction instrument drive shaft and measures, records and outputs the rotation angle value of the library construction instrument drive shaft; The driving displacement module includes a first rotating connecting shaft and a second rotating connecting shaft; the first rotating connecting shaft drives the simulated suction head seat assembly to perform horizontal left-right displacement by forward and reverse rotation, and the second rotating connecting shaft drives the simulated suction head probe in the simulated suction head seat assembly to perform vertical up-down displacement by forward and reverse rotation; The debugging module includes a first trigger baffle, a first measuring device and a second trigger baffle corresponding to the first photoelectric switch and the second photoelectric switch respectively; the first trigger baffle is fixedly arranged on the simulated suction head seat assembly, and the first measuring device is a linear measuring device; the second trigger baffle is fixedly arranged on the library construction instrument driving shaft connected to and driving the second rotating connecting shaft; The debugging module also includes a second measuring device corresponding to the second photoelectric switch, and the second measuring device is a rotation angle measuring device.

2. The tooling according to claim 1, characterized in that: The linear measuring device includes a capacitive scale, a grating scale, a magnetic scale or a laser displacement sensor; the rotation angle measuring device includes a magnetic sensitive angle sensor.

3. The tooling according to claim 1, characterized in that: The horizontal debugging position of the debugging hole corresponding to the simulated suction head seat assembly for simulated operation is the side projection vertical line distance from the first photoelectric switch trigger position to the center line of the debugging hole; A contact detection end is provided at the center of the inner bottom surface of the debugging hole, and the vertical debugging position of the debugging hole corresponding to the simulated suction head seat assembly for simulated operation is the vertical distance between the simulated suction head probe and the contact detection end when the simulated suction head probe moves vertically upward to the limit position.

4. The tooling according to any one of claims 1 to 3, characterized in that: Also includes a housing; The driving displacement module, the debugging hole and the simulated suction head seat assembly are arranged in the housing; The shell size and the opening position are compatible with the library construction instrument card box.

5. A method for debugging a library building instrument, characterized in that: Use the tooling debugging library construction instrument drive shaft as described in any one of claims 1 to 4 to rotate and drive the displacement distance and / or displacement zero point position of the suction head seat assembly in the library construction instrument cartridge.

6. The method according to claim 5, characterized in that include: Insert the library construction instrument debugging tool into the library construction instrument and connect the library construction instrument drive shaft and the tool rotation connection shaft; According to the library construction instrument control program, the simulated suction head holder assembly is driven to respectively perform the simulated operations of the horizontal debugging position and the vertical debugging depth of the corresponding debugging hole position; Calculate assembly errors by simulating the displacement distance actually performed by the tip holder assembly output by the measuring device; Use assembly error to adjust the library construction instrument control program settings; According to the adjusted library construction instrument control program settings, the simulation operation is performed again and the new assembly error is recalculated until the assembly error is less than or equal to the preset allowable value.

7. The method according to claim 6, characterized in that The use of assembly error to adjust the library building instrument control program setting includes adjusting the zero point position of the displacement of the tip holder assembly in the library building instrument cartridge, and / or adjusting the correspondence between the rotation angle of the library building instrument drive shaft and the displacement distance of the tip holder assembly in the library building instrument cartridge.

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