A device and method for digitizing a liquid sample

Abstract

The application provides a liquid sample digitizing device and method, wherein the device comprises a container, the container is provided with at least one digitizing channel for containing a liquid sample, the diameter of the digitizing channel is less than or equal to a preset value to limit the diffusion of the digitized liquid sample in the digitizing channel; and a heating assembly, the heating assembly at least partially heats the digitizing channel, and the heating temperature of the heating assembly is not lower than the cloud point temperature of the liquid sample. According to the technical scheme of the application, after the liquid sample to be digitized is filled into the digitizing channel with a preset size, the digitizing channel is heated at a temperature higher than the cloud point of the liquid sample, and the digitizing process of the liquid sample to be digitized is realized. Compared with the prior art, the overall operation of the application is simple, the container, the heating assembly and the like used in the application are relatively low-priced conventional instruments, and the digitizing operation of the liquid sample can be realized in a short time.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sample and reagent processing, in particular to a liquid sample digitization device and method. BACKGROUND

[0002] Liquid digitization is the distribution and analysis of liquid and entities (such as particles, microorganisms, cells, molecules, etc.) therein. In the process of liquid digitization, spatial distribution technology plays a crucial role, which is to distribute entities in a solution or suspension into different subunits. Currently, the commonly used digitization technologies in liquid digitization mainly include microcavity technology and microdroplet technology.

[0003] The microcavity technology is based on an integrated fluid channel chip, which relies on microstructures on the chip to divide the liquid into independent microdroplet units. However, when using the microcavity technology for digitization operation, the operation is time-consuming, the reaction cost is high, and the structure of the equipment used to realize the digitization operation is complex.

[0004] The microdroplet technology is based on the principle of water-in-oil, which uses shear force to disperse the liquid into an immiscible oil phase to form water-in-oil microdroplets. However, when using the microdroplet technology for digitization operation, a large amount of oil phase and surfactant is required, which is costly.

[0005] Overall, the structure of the digitization equipment used to realize liquid digitization is complex, and the equipment cost and process cost are high. SUMMARY

[0006] The present application provides a liquid sample digitization device and method to solve the defects of complex digitization operation, long time-consuming, and high reaction cost in the prior art, and to realize simple operation, low cost, and short time-consuming for liquid sample digitization processing.

[0007] The present application provides a liquid sample digitization device, which comprises:

[0008] A container provided with at least one digitization channel for containing a liquid sample, the diameter of the digitization channel is less than or equal to a preset value to limit the diffusion of the digitized liquid sample in the digitization channel.

[0009] A heating assembly at least partially heats the digitization channel, and the heating temperature of the heating assembly is not lower than the cloud point temperature of the liquid sample.

[0010] According to the liquid sample digitization device provided by the present application, the extension direction of the digitization channel comprises at least one low-temperature zone and at least one high-temperature zone, the heating assembly is arranged in each high-temperature zone, and the high-temperature zones are arranged at intervals with the low-temperature zones.

[0011] The liquid sample digitization device provided by the application comprises a low-temperature area and a high-temperature area.

[0012] The container comprises a micro-channel chip, and micro-channels of the micro-channel chip form the digitization channel.

[0013] The heating assembly is a laser source and a thermal light source, and the heating assembly is located at one side of the micro-channel chip where the micro-channels are formed.

[0014] The liquid sample digitization device provided by the application comprises a low-temperature area and a high-temperature area.

[0015] The light-shielding plate is located at one side of the micro-channel chip where the phase separation channel is formed, and the light-shielding plate is located between the micro-channel chip and the heating assembly.

[0016] The light-shielding plate is located at one side of the micro-channel chip where the phase separation channel is formed, and the light-shielding plate is located between the micro-channel chip and the heating assembly.

[0017] The cooling assembly is a water cooling box or a cooling substrate.

[0018] The container comprises a micro-channel pipe, and a pipe line of the micro-channel pipe forms the digitization channel.

[0019] The heating assembly is an electric heating sheet, and the electric heating sheet is arranged in the high-temperature area of the digitization channel.

[0020] The container comprises a capillary coil, and a pipe line of the capillary coil forms the digitization channel.

[0021] The heating assembly is an electric heating support, the capillary coil is installed on the electric heating support, and a contact position of the electric heating support and the capillary coil is the high-temperature area of the digitization channel.

[0022] The application further provides a liquid sample digitization method based on the liquid sample digitization device.

[0023] The liquid sample is filled into the digitization channel of the container with a preset size.

[0024] The heating assembly is used to heat different positions of the digitization channel of the container until a plurality of water phases and glue phases alternately appear in the digitization channel.

[0025] The liquid sample is a colloidal solution exceeding a critical micelle concentration, and the heating temperature of the heating assembly is not lower than the cloud point temperature of the liquid sample.

[0026] The liquid sample digitization device and method provided by the application can directly digitize the liquid sample in the container, and the overall operation is simple. The container, the heating assembly and the like used are relatively low-priced conventional instruments, and the digitization operation of the liquid sample to be digitized can be realized in a short time. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0028] Figure 1 is one of the digitization principle structure schematic diagrams of the liquid sample digitization device provided by the application;

[0029] Figure 2 is the other digitization principle structure schematic diagram of the liquid sample digitization device provided by the application;

[0030] Figure 3 is the structure schematic diagram of the liquid sample digitization in the preset size container provided by the application;

[0031] Figure 4 is the structure schematic diagram of the liquid sample digitization device provided by the embodiment 1 of the application;

[0032] Figure 5 is the structure schematic diagram of the liquid sample digitization device provided by the embodiment 2 of the application;

[0033] Figure 6 is the other structure schematic diagram of the liquid sample digitization device provided by the embodiment 2 of the application;

[0034] Figure 7 is the other structure schematic diagram of the liquid sample digitization device provided by the embodiment 2 of the application;

[0035] Figure 8 is the structure schematic diagram of the liquid sample digitization device provided by the embodiment 3 of the application;

[0036] Figure 9 is another structural schematic diagram of a liquid sample digitization device provided by embodiment 3 of the present application;

[0037] Figure 10 is a structural schematic diagram of a liquid sample digitization device provided by embodiment 4 of the present application;

[0038] Figure 11 is a flow schematic diagram of a liquid sample digitization method provided by the present application.

[0039] Reference signs:

[0040] 11: micellar phase microdroplet; 12: aqueous phase; 21: aqueous phase; 22: micellar phase; 31: small size container; 32: aqueous phase microdroplet; 33: micellar phase microdroplet; 41: fan; 42: aqueous phase; 43: gel phase; 44: microchannel tube; 45: electric heating sheet; 51: light source; 52: light schematic; 53: mask; 54: mask slot hole; 55: microchannel chip; 56: refrigeration system; 61: transparent effect mask; 62: microchannel; 71: light schematic; 72: gel phase; 73: transparent mask schematic; 74: aqueous phase; 81: capillary coil; 82: heating support; 83: fan; 84: fan cooling air flow; 91: gel phase; 92: aqueous phase; 101: laser source; 102: galvanometer scanning lens; 103: laser beam; 104: microchannel chip quartz cover; 105: gel phase; 106: aqueous phase; 107: microchannel chip; 108: constant temperature water cooling box. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions in the present application will be described clearly and completely below in combination with the drawings in the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0042] The present application will be described below in combination with Figures 1-10 A liquid sample digitization device is described.

[0043] Nonionic surfactants are surfactants containing ether groups in the molecule which do not dissociate in aqueous solution as the main hydrophilic group, and the surface activity of which is manifested by neutral molecules. Nonionic surfactants have very high surface activity, good solubilization, washing, antistatic, calcium soap dispersion and other properties, less irritating, and excellent wetting and washing functions. The pH value range can be wider than that of general ionic surfactants, and it can also be used with other ionic surfactants. Adding a small amount of nonionic surfactant to the ionic surfactant system can increase the surface activity of the system. Nonionic surfactants can be divided into polyoxyethylene type, polyol type, alkanolamide type, polyether type, amine oxide type and the like according to the structure of the hydrophilic group.

[0044] The clear and uniform nonionic surfactant solution (the nonionic surfactant can be polyoxyethylene type, polyol type, polyether type, amine oxide type, or a mixture containing these surfactants) will cause the solubility to decrease due to changes in temperature and other conditions, and spontaneously form two incompatible phases, micellar phase and water phase with lower surfactant concentration, respectively. The threshold temperature at which phase separation begins is called the cloud point, and the phase separation process is a reversible physical change. It is generally believed that this phenomenon is caused by the growth and structural change of micelles, the connection of micelles into a network, and the H-bonding between water molecules and micelles.

[0045] As shown in Figure 1 , the digitization process completed in a macroscopic container first forms micellar phase microdroplets 11 and water phase 12, and the apparent manifestation is that the solution becomes turbid.

[0046] As shown in Figure 2 , then due to the effect of diffusion, the micellar phase microdroplets 11 gradually aggregate, and finally form two independent clear macroscopic phases, water phase 21 and micellar phase 22.

[0047] As shown in Figure 3 , the present application transfers the digitization process to a small size container 31, which can be a capillary or a microchannel, and at least one dimension of the size is <1mm. When the temperature reaches the cloud point and starts digitization, the water phase microdroplets 32 and the micellar phase microdroplets 33 formed are difficult to aggregate due to the diffusion barrier in the small size container, and the micellar phase and the water phase will form multiple independent micellar phase and water phase regions in the small size container, thereby realizing the uniform separation of the water phase, that is, the digitization process. This digitization method is short in time, low in cost, simple in operation, and significantly better than the current two methods of digitizing aqueous solutions; in addition, when the solution temperature decreases, the micellar phase and the water phase will reversibly transform into a single phase, which is convenient for subsequent operations.

[0048] To regulate the liquid sample digitization process, different temperature gradients can also be applied on the small size container, so that the micelle phase is only generated in the area where the temperature is higher than the cloud point, so that the digitization process can be better controlled. The high temperature part of the temperature gradient can be realized by selective resistance heating Figure 4 as shown, or selective light heating Figure 5 The low temperature part of the temperature gradient can be realized by air cooling, water cooling or diode cooling.

[0049] The embodiment of the present application provides a liquid sample digitization device, which comprises a container and a heating assembly. The container is provided with at least one digitization channel for containing a liquid sample, and the diameter of the digitization channel is less than or equal to a preset value to limit the diffusion of the digitized liquid sample in the digitization channel. The heating assembly at least partially heats the digitization channel, and the heating temperature of the heating assembly is not lower than the cloud point temperature of the liquid sample. In some embodiments, the preset value can be greater than or equal to 1 micrometer and less than or equal to 1 millimeter. By filling the liquid to be digitized into the digitization channel with a preset size, and then heating the digitization channel above the cloud point of the liquid sample, the digitization of the liquid sample to be digitized can be realized. Compared with the prior art, the present application can directly digitize in the container, the overall operation is simple, the container, the heating assembly and the like used are relatively low-priced conventional instruments, and the digitization operation of the liquid sample to be digitized can be realized in a short time.

[0050] In the present application, the liquid sample is a colloidal solution exceeding the critical micelle concentration. The critical micelle concentration (English: Critical micelle concentration, often abbreviated as CMC) is defined as one of the most important physical quantities characterizing the structure and performance of surfactants. According to the CMC value of the surfactant, the amount of surfactant added can be designed to obtain a solution with controllable micelle size and shape. The minimum concentration of surfactant required for surfactant to form micelles is called critical micelle concentration.

[0051] The cloud point is a characteristic constant of non-ionic surfactants, which is affected by the molecular structure of the surfactant and the coexisting substances. The aqueous solution of the surfactant will appear turbidity with the increase of temperature, and the surfactant will change from complete dissolution to partial dissolution, and the temperature at which the change occurs is the cloud point temperature. The cloud point is the temperature at which the homogeneous micelle solution of non-ionic surfactant undergoes phase separation.

[0052] In the liquid sample digitization device of the present invention, in order to better realize the digitization of liquid samples, the digitization channel extends in a direction including at least one low-temperature zone and at least one high-temperature zone. Heating components are disposed in each high-temperature zone, and the high-temperature zone and the low-temperature zone are spaced apart. Specifically, a cooling component is disposed in each low-temperature zone. By disposing of the cooling component, the temperature in other locations within the digitization channel remains substantially constant, except for the high-temperature zone heated by the heating component. This allows the liquid sample to be digitized within the preset high-temperature zone without spreading to other locations within the digitization channel.

[0053] Optionally, in this invention, the cooling component is a water-cooled box, a cooling substrate, a fan, or other devices that can achieve a cooling effect, and the cooling substrate is a semiconductor cooler.

[0054] like Figure 4 As shown, the container also includes microchannels, the tubing of which forms digital channels, allowing solution samples to fill the tubing of the microchannels.

[0055] Specifically, such as Figure 4 As shown, in one embodiment of the present invention, the heating component is a heating element 45. The liquid sample is filled in a microchannel tube 44, and the channels of the microchannel tube 44 form a digital channel. The heating element 45 is disposed in the high-temperature zone of the digital channel, and the part of the heating element 45 in contact with the microchannel tube 44 is the high-temperature zone of the microchannel tube 44. The heating element 45 heats the liquid sample in the microchannel tube 44, causing the temperature to rise to the cloud point temperature, thereby separating it into an aqueous phase 42 and a colloidal phase 43. A fan 41 is also provided to cool the low-temperature zone of the microchannel tube by rotating and blowing air.

[0056] like Figures 5-7 As shown, the container in this invention includes a microchannel chip, and the microchannels of the microchannel chip form digital channels. The heating component is a laser source or a heat source, and is located on the side of the microchannel chip where the microchannels are formed, for heating the high-temperature part of the digital channels.

[0057] The digitization device also includes: a light-shielding plate located on the side of the microchannel chip where the digitization channel is formed, and positioned between the microchannel chip and the heating component; the light-shielding plate has a light-transmitting groove, which is correspondingly positioned to the digitization channel. This allows the heat from the thermal light source to pass through the light-transmitting groove and irradiate the high-temperature area of ​​the digitization channel.

[0058] The microchannel chip consists of multiple microchannels, each with a diameter of no more than one millimeter, which enables the technical effect of preventing the diffusion of digitized liquid samples within the digital channels.

[0059] Specifically, such as Figure 5 , 6As shown in Figure 7, in another embodiment of the present invention, the heating component is a thermal light source 51, as illustrated in the light schematic 52. The container includes a microchannel chip 55, and the microchannels 62 of the microchannel chip 55 form digital channels. The thermal light source 51 is located on the side of the microchannel chip 55 where the microchannels 62 are formed. The light-shielding plate is a mask 53, located on the side of the microchannel chip 55 where the digital channels are formed, and the mask 53 is located between the microchannel chip 55 and the thermal light source 51. The mask 53 has mask slots 54, which are correspondingly arranged with the digital channels.

[0060] The microchannel chip 55 is mounted on the cooling system 56, such as... Figure 7 , 8 The transparent mask 61, the transparent mask schematic 73, and the light schematic 71 shown are illustrated in this embodiment. In this embodiment, the thermal light source 51 irradiates different positions of the microchannel 62 of the microchannel chip 55 through the mask slot 54. These different positions are heated after being irradiated and are the high-temperature parts of the digitization channel. When the temperature rises to the cloud point temperature of the liquid sample, the liquid sample begins to be digitized and is divided into a colloidal phase 72 and an aqueous phase 74.

[0061] like Figure 11 As shown, applying the liquid sample digitization device provided in the embodiments of the present invention, the present invention also provides a liquid sample digitization method, which includes the following steps:

[0062] 1101. Fill the liquid sample into the digital channel of the container of a preset size; specifically, place the preset amount of solute and solution in a centrifuge and centrifuge at 3000G speed for 5 minutes to obtain the liquid sample.

[0063] 1102. Use the heating assembly to heat different positions of the digital channel of the container until multiple alternating aqueous and colloidal phase separation spaces appear in the digital channel;

[0064] The liquid sample is a colloidal solution exceeding the critical micelle concentration, and the heating temperature of the heating component is not lower than the cloud point temperature of the liquid sample. This invention proposes a novel liquid digitization method, specifically a novel liquid sample digitization method. It utilizes the turbidity phenomenon produced when a colloidal solution exceeding the critical micelle concentration is heated above its cloud point. Heating the colloidal solution in a small channel causes it to spontaneously separate into two phases. Due to impeded diffusion in the small-sized channel, the two phases will appear alternately, thereby separating the aqueous phase into uniform microdroplets, achieving the digitization of the aqueous solution.

[0065] Specifically, the use is as follows Figure 5 , 6The specific operation of the liquid sample digitization device shown in Figure 7 for digitizing liquid samples includes: thoroughly vortexing 1g of Triton X-114 with 5ml of cool water, centrifuging at 3000G for 5 minutes, and then filling the microchannel chip 55 with the mixed solution. The microchannel chip 55 is placed on a copper thermally conductive substrate cooled by a diode refrigeration chip, and the substrate temperature is controlled at 5-10 degrees Celsius. A slotted tin foil mask 53 covers the microchannel chip 55. A high-power thermal light source 51 is used to irradiate the chip mask 53 in the visible to infrared band above the microchannel chip 55. Some of the light passes through the mask slots 54 and irradiates the microchannel area of ​​the chip, raising the temperature of the solution inside the microchannel. When the temperature of the irradiated area in the channel reaches 22°C, a gel phase 72 forms in these areas of the channel, dividing the channel into multiple alternating intervals of aqueous phase 74 and gel phase 72.

[0066] like Figure 8 , 9 As shown, the container also includes a capillary coil, the tubing of which forms a digital channel to fill the liquid sample into the tubing formed by the capillary coil.

[0067] Meanwhile, the heating component is an electric heating bracket, and the capillary coil is installed on the electric heating bracket. The contact position between the electric heating bracket and the capillary coil is the high-temperature zone of the digital channel. The temperature of the heating bracket is controlled so that the temperature of the high-temperature zone can reach the cloud point temperature.

[0068] Specifically, such as Figure 8 , 9 As shown, in another embodiment of the present invention, the heating component is a heating bracket 82, which heats up after being energized. The container includes a capillary coil 81, the tubing of which forms a digital channel. The capillary coil 81 is mounted on the electric heating bracket 82, and the contact point between the electric heating bracket 82 and the capillary coil 81 is the high-temperature zone of the digital channel.

[0069] In an embodiment of the present invention, the cooling component is a fan 83, such as... Figure 8 The image shows the fan cooling airflow 84, which blows towards the capillary coil 81. This airflow, in conjunction with the heating bracket 82, creates a temperature gradient between high-temperature and low-temperature zones within the digitization channel. When the heating bracket 82 is energized, it heats different locations within the digitization channel of the capillary coil 81. Once the temperature at these locations rises to the cloud point temperature of the liquid sample, the liquid sample begins to digitize, separating into a colloidal phase 91 and an aqueous phase 92.

[0070] Specifically, the use is as follows Figure 8 , 9The specific operation of the liquid sample digitization device shown for digitizing a liquid sample includes: 1 g of polysorbate 80 is mixed with 5 ml of water by vortexing, and then centrifuged at 3000 G for 5 minutes, after which the mixed solution is filled into a quartz capillary coil 81. The quartz capillary coil is supported by a heating support 82, and a fan 83 can be used to air-cool the entire quartz capillary coil. When the heating support 82 is powered on, the support generates heat and heats the contact part of the quartz capillary coil. At this time, the fan 83 blows air on the entire quartz capillary coil 81 to keep the overall temperature of the coil constant. The heating power of the support and the air volume of the fan are controlled to make the temperature of the quartz capillary coil and the directly contacted part rise, and the temperature of other parts of the coil remain constant. When the temperature of the quartz capillary coil and the directly contacted part reaches 95℃, the gel phase 91 in the quartz capillary is generated. The entire quartz capillary channel regularly generates multiple gel phase and water phase separation intervals.

[0071] As shown in Figure 10 In another embodiment of the present application, the heating assembly is a laser source 101, and a galvanometer scanning lens 102 is arranged on the laser source 101, and a laser beam 103 for heating is emitted from the laser source 101.

[0072] The container includes a microchannel chip 107, and the microchannels of the microchannel chip 107 form digitization channels. The laser source 101 is located on the side of the microchannel chip 107 where the microchannels are opened. Among them, the microchannel chip 107 is covered with a microchannel chip quartz cover 104, and the microchannel chip 107 is arranged in a constant-temperature water-cooled box 108. In this embodiment, the laser source 101 is modulated so that the laser beam 103 emitted from the galvanometer scanning lens is irradiated on different positions of the microchannels of the microchannel chip 107. The different positions are heated after being irradiated by the laser beam 103, and are high-temperature parts of the digitization channels. When the temperature rises to the cloud point temperature of the liquid sample, the liquid sample begins to digitize and is divided into a gel phase 105 and a water phase 106.

[0073] Specifically, as shown in Figure 10The specific operation of the liquid sample digitization device for digitizing a liquid sample includes: 1 g of hydrogenated castor oil polyoxyethylene ether EL-30 is fully vortex mixed with 5 ml of water, centrifuged at 3000 G for 5 minutes, and then the mixed solution is filled in the microchannel chip 107. The upper layer of the microchannel chip is a quartz cover plate. The microchannel chip is placed on a circulating water-cooled constant temperature 25℃ substrate or a constant temperature water-cooled box 108. Above the microchannel chip is a laser system configured with a galvanometer scanning lens 102, which can irradiate and generate heat on any point, line or surface on the chip. When adjusting the power of the laser, irradiate the specific position of the microchannel on the chip, the laser beam 103 irradiates the quartz cover 104 of the microchannel chip to heat the cover plate. Using different quartz cover plates or using different wavelength lasers, the solution in the channel can be directly heated through the cover plate. When the temperature of the solution in the irradiated area of the channel reaches 95℃, the gel phase is formed in these areas of the channel, and the channel is divided into multiple separated areas with alternating water phase and gel phase.

[0074] The present application provides a new liquid digitization method, namely a new liquid sample digitization device and method. The method utilizes the phenomenon that a colloidal solution with a concentration exceeding the critical micelle concentration becomes turbid when heated above the cloud point. The colloidal solution is heated in a small channel, so that the colloidal solution spontaneously separates into two phases. Due to the blocked diffusion in the small channel, the two phases will appear separately, thereby separating the water phase into uniform microdroplets, and realizing the digitization of the aqueous solution.

[0075] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and are not limited thereto; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing examples, or make equivalent substitutions for part of the technical features; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A liquid sample digitizing device, characterized by, The device comprises: a container provided with at least one digitization channel for containing a liquid sample, the digitization channel having a size less than or equal to a preset value to limit the diffusion of the digitized liquid sample in the digitization channel; a heating assembly at least partially heating the digitization channel, and the heating temperature of the heating assembly being not lower than the cloud point temperature of the liquid sample; wherein the liquid sample is a colloidal solution exceeding a critical micelle concentration; the heating assembly is used to heat different positions of the digitization channel of the container, and when the temperature reaches the cloud point to start digitization, water phase microdroplets and micellar phase microdroplets formed in the small size container form a plurality of independent micellar phase and water phase zones; wherein the extension direction of the digitization channel comprises at least one low temperature zone and at least one high temperature zone, the heating assembly is arranged in each high temperature zone, and the high temperature zone is arranged in the low temperature zone. wherein each low temperature zone is provided with a cooling assembly.

2. The liquid sample digitization device according to claim 1, wherein the container comprises a microchannel chip, and the microchannel of the microchannel chip forms the digitization channel.

3. The liquid sample digitization device according to claim 2, wherein the heating assembly is a laser source and a thermal light source, and the heating assembly is located on the side of the microchannel chip where the microchannel is opened.

4. The liquid sample digitizing device of claim 3, wherein, The device further comprises: a light shield plate located on the side of the microchannel chip where the phase separation channel is opened, and the light shield plate is located between the microchannel chip and the heating assembly; the light shield plate is provided with a light transmission groove corresponding to the digitization channel.

5. The liquid sample digitization device according to claim 1, wherein the cooling assembly is a water cooling box or a cooling substrate.

6. The liquid sample digitization device according to claim 1, wherein the container comprises a microchannel tube, and the pipeline of the microchannel tube forms the digitization channel; the heating assembly is an electric heating sheet, and the electric heating sheet is arranged in the high temperature zone of the digitization channel.

7. The liquid sample digitization device according to claim 1, wherein the container comprises a capillary coil, and the pipeline of the capillary coil forms the digitization channel; the heating assembly is an electric heating bracket, the capillary coil is installed on the electric heating bracket, and the contact position of the electric heating bracket and the capillary coil is the high temperature zone of the digitization channel.

8. A method of digitizing a liquid sample based on the liquid sample digitizing device of any one of claims 1-7, characterized in that, The method comprises: filling the liquid sample into the preset size digitization channel of the container; using the heating assembly to heat different positions of the digitization channel of the container until a plurality of water phase, gel phase alternating separation spaces appear in the digitization channel; wherein the liquid sample is a colloidal solution exceeding a critical micelle concentration, and the heating temperature of the heating assembly is not lower than the cloud point temperature of the liquid sample.

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