Spotting equipment

Abstract

The present application discloses a spotting device, which belongs to the field of biological experimental equipment. The spotting device includes a temperature control component and a spotting device. The temperature control component includes a first temperature control component and a temperature storage component. The first temperature control component is used to adjust the temperature of the temperature storage component. The spotting device includes a driving mechanism and a spotting head. The spotting head is connected to the driving mechanism. The driving mechanism is used to adjust the position of the spotting head so that the spotting head can be combined with the temperature storage component. The spotting gun head of the spotting head is equipped with materials for spotting. The spotting head of the spotting device has the advantages of precise temperature control, simple structure and light weight.

Description

Technical Field

[0001] The present application relates to the field of biological experimental equipment, and in particular to a spotting device. Background Art

[0002] The spotting device is a commonly used experimental equipment, which is used to spot the spotting material at a designated location. For some spotting materials, due to their special physical and chemical properties, it is necessary to ensure that the spotting materials are in a suitable temperature environment during spotting. For example, in the field of organoid culture, in the process of making organoids, it is necessary to mix cells with matrix gel, and quantitatively spot the spotting materials on the carrier plate through the spotting device to form cell-matrix gel mixed microspheres. During the spotting process, the spotting materials on the carrier plate need to be kept in an environment of about 37°C, and the spotting materials in the spotting device need to be kept in an environment of about 4°C. In view of this, in the prior art, in order to ensure that the spotting materials in the spotting device are in a suitable temperature environment, the spotting head of the spotting device is usually provided with a temperature control component. The defect of such a setting is that the spotting head includes a variety of components, which makes the spotting head structure complicated, too heavy, difficult to sterilize, and reduced reliability. Summary of the invention

[0003] The purpose of the present application is to provide a spotting device, wherein the spotting head of the spotting device has the advantages of simple structure and reliable operation.

[0004] The present application provides a sample spotting device, comprising a temperature control component and a sample spotting device, wherein the temperature control component comprises a first temperature control component and a temperature storage component, wherein the temperature storage component can be detachably arranged on the first temperature control component, and the first temperature control component is used to adjust the temperature of the temperature storage component; the sample spotting device comprises a driving mechanism and a sample spotting head, wherein the sample spotting head is connected to the driving mechanism, and the driving mechanism is used to adjust the position of the sample spotting head so that the sample spotting head can be detachably connected to the temperature storage component.

[0005] In the above scheme, the temperature of the temperature storage element can be adjusted by the first temperature control component. After the temperature storage element is adjusted to a suitable temperature under the action of the first temperature control component, the position of the spotting head can be adjusted by the driving mechanism, so that the temperature storage element can be detachably connected to the spotting head. Through the action of heat conduction, the temperature of the spotting material in the spotting head can be maintained within the set temperature range for a period of time. Since the spotting head, the temperature storage element and the first temperature control component are independent components, that is, the temperature storage element can be detached from the spotting head, and the first temperature control component is not integrated in the spotting head, the structure of the spotting head is simplified and the reliability of the spotting head is improved.

[0006] In some embodiments, the temperature storage component includes a first temperature storage block and a second temperature storage block, and the first temperature storage block and the second temperature storage block are detachably connected so that the first temperature storage block and the second temperature storage block are connected to the spotting head.

[0007] In the above scheme, the first temperature storage block and the second temperature storage block can be closed to clamp the spotting head between the first temperature storage block and the second temperature storage block, and the first temperature storage block and the second temperature storage block can be fixed to the spotting head. Conversely, when disassembling the first temperature storage block and the second temperature storage block, the first temperature storage block and the second temperature storage block can be separated. The detachable connection between the first temperature storage block and the second temperature storage block can realize the rapid disassembly and assembly of the temperature storage parts, which is convenient to use. In addition, the first temperature storage block and the second temperature storage block can be sterilized by high temperature and high pressure sterilization or ozone, hydrogen peroxide sterilization and other sterilization methods, which improves the sterilization effect and reduces the risk of contamination of the spotting material.

[0008] In some embodiments, the first temperature control assembly includes a first temperature control unit and a second temperature control unit, and the first temperature control unit and the second temperature control unit are used to adjust the temperatures of the first temperature storage block and the second temperature storage block respectively.

[0009] In some embodiments, the temperature control assembly also includes a base, a first rotating seat, and a second rotating seat, and the first rotating seat and the second rotating seat are both rotatably arranged on the base; along the first direction, the first temperature control unit and the second temperature control unit are arranged on the base, and the first rotating seat and the second rotating seat are arranged between the first temperature control unit and the second temperature control unit, the first rotating seat is used to accommodate two first temperature storage blocks, and the second rotating seat is used to accommodate two second temperature storage blocks; wherein, the first rotating seat is used to switch the positions of the two first temperature storage blocks so that the two first temperature storage blocks face the first temperature control unit and the second temperature storage block respectively; the second rotating seat is used to switch the positions of the two second temperature storage blocks so that the two second temperature storage blocks face the second temperature control unit and the first temperature storage block respectively.

[0010] In the above scheme, since the two first temperature storage blocks and the two second temperature storage blocks are respectively provided with the first rotating seat and the second rotating seat, during operation, the two rotating seats are rotated so that the first temperature storage block provided on the first rotating seat can face the first temperature control unit and be cooled by the first temperature control unit; and the second temperature storage block provided on the second rotating seat can face the second temperature control unit and be cooled by the second temperature control unit, thereby achieving the purpose of adjusting the temperature of the first temperature storage block and the second temperature storage block. Furthermore, the position of the first temperature storage block is adjusted by the first rotating seat, and the position of the second temperature storage block is adjusted by the second rotating seat, so that one of the two first temperature storage blocks and one of the two second temperature storage blocks can be arranged relative to each other, so that the first temperature storage block and the second temperature storage block can be conveniently installed on the spotting head.

[0011] In some embodiments, along the first direction, the first rotating seat and the second rotating seat are both adjustable in position on the base.

[0012] In the above scheme, the positions of the first rotating seat and the second rotating seat are adjustable relative to the base. On the one hand, rotating the first rotating seat and the second rotating seat can make one of the two first temperature storage blocks on the first rotating seat face one of the two second temperature storage blocks on the second rotating seat, so as to facilitate fixing the first temperature storage block and the second temperature storage block to the spotting head; on the other hand, it is convenient to adjust the positions of each temperature storage block and the corresponding temperature control unit, so that the first temperature control unit can abut against the first temperature storage block, and the second temperature control unit can abut against the second temperature storage block.

[0013] In some embodiments, the spotting head includes a spotting gun head and a piston rod, the piston rod is movably disposed in the spotting gun head, and the spotting gun head is used to be combined with the temperature storage component.

[0014] In the above scheme, by moving the piston rod, the spotting gun head can draw in the spotting material or the spotting material in the spotting gun head can be squeezed out by the piston rod. In addition, by controlling the position of the piston rod relative to the spotting gun head, the volume of the squeezed spotting material can be controlled to achieve the purpose of quantitatively dropping the spotting material. Since the spotting material in the spotting gun head is relatively small, the temperature of the spotting material in the spotting gun head changes rapidly, so the temperature storage part is matched with the spotting gun head to avoid the denaturation of the spotting material in the spotting gun head.

[0015] In some embodiments, the spotting device further comprises a power mechanism, which is connected to the driving mechanism and is used to push the piston rod to move relative to the spotting gun head.

[0016] In the above scheme, the purpose of driving the piston rod to move relative to the spotting gun head is achieved through the power mechanism, thereby achieving the purpose of the spotting head drawing in or dripping the spotting material.

[0017] In some embodiments, the spotting device also includes a temperature control platform, the temperature control platform includes a third temperature control unit, a fourth temperature control unit, a storage platform and a spotting platform, the storage platform is used to store the spotting material, the third temperature control unit is arranged at the bottom of the spotting platform, and the fourth temperature control unit is arranged at the bottom of the storage platform; wherein the driving mechanism is used to adjust the position of the spotting head so that the spotting head can transfer the spotting material in the storage platform to the spotting platform.

[0018] In the above solution, the third temperature control unit keeps the sample material disposed on the sample dispensing platform at a set temperature environment, and the fourth temperature control unit keeps the carrier plate disposed on the storage platform at a set temperature environment. The sample dispensing head can move under the action of the driving mechanism.

[0019] In some embodiments, the driving mechanism is detachably connected to the spotting head; and the storage platform is provided with a placement slot for placing the spotting gun head.

[0020] In the above scheme, the spotting head to be used is placed in the placement slot so that the spotting head can be kept at a set temperature, thereby preventing the spotting material from being denatured due to inappropriate temperature when the spotting head draws in the spotting material.

[0021] In some embodiments, the third temperature control unit includes a first semiconductor temperature control plate, and the fourth temperature control unit includes a second semiconductor temperature control plate; the first semiconductor temperature control plate includes a first working surface and a first non-working surface that are opposite to each other, and the second semiconductor temperature control plate includes a second working surface and a second non-working surface that are opposite to each other; the first working surface of the first semiconductor temperature control plate abuts against the spotting platform, and the second working surface of the second semiconductor temperature control plate abuts against the storage platform, and the first non-working surface of the first semiconductor temperature control plate and the second non-working surface of the second semiconductor temperature control plate are connected by a heat conducting element.

[0022] In the above scheme, the first working surface of the first semiconductor temperature control board and the second working surface of the second semiconductor temperature control board can be heated or cooled; the heat conducting element can maintain the first non-working surface of the first temperature control board and the second non-working surface of the second temperature control board at the same temperature; the temperature regulating component is used for heat exchange between the heat conducting element and the external environment, which on the one hand improves the working efficiency of the first semiconductor temperature control board and the second semiconductor temperature control board, and on the other hand reduces the disturbance to the experimental environment and improves the stability of the experimental environment.

[0023] The spotting device of the present application has the following beneficial effects:

[0024] The present application uses a temperature storage component to keep the sample spotting head warm, that is, the temperature control component and the sample spotting head are separated, which reduces the mass of the sample spotting head, realizes the lightweight of the sample spotting head, and increases the moving speed and sample spotting efficiency of the sample spotting head;

[0025] Since multiple temperature storage parts can be set as needed, the number is not limited and they can be used at any time according to needs, thus avoiding the influence of heating or cooling process on material properties or cell activity;

[0026] The temperature control component controls the temperature of the temperature storage component. The rotating clamping design of the temperature control component is used to circulate the temperature of multiple temperature storage components. The standby temperature storage components are in a charged state. When they need to be used, they are clamped to the spotting head, so that the spotting head can have a continuous supply of temperature storage components. This modular design of the temperature storage components and the spotting head that can be detached and combined makes it possible to quickly complete the spotting of a group or a batch of cell microspheres.

[0027] The first non-working surface of the first semiconductor temperature control plate and the second non-working surface of the second semiconductor temperature control plate of the temperature control platform are connected by heat conduction through a heat conducting element, which can improve the working efficiency of the first semiconductor temperature control plate and the second semiconductor temperature control plate. This design can not only save energy to the maximum extent, but also minimize the disturbance to the experimental environment and maintain the stability of the environment required during the sampling process.

[0028] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present application. Moreover, the same reference numerals are used throughout the drawings to represent the same components. In the drawings:

[0030] Figure 1 This is a schematic diagram of the structure of the spotting device in some embodiments of the present application;

[0031] Figure 2 This is a schematic diagram of the front view structure of the spotting device of some embodiments of the present application;

[0032] Figure 3 It is a schematic diagram of the side view structure of the spotting device in some embodiments of the present application;

[0033] Figure 4 Schematic diagram of a spotting gun tip and a piston rod in some embodiments of the present application;

[0034] Figure 5 This is a schematic diagram of the temperature control component structure of some embodiments of the present application;

[0035] Figure 6 This is a schematic diagram of the temperature control platform structure of some embodiments of the present application.

[0036] The reference numerals in the specific implementation manner are as follows:

[0037] 100-spotting device; 200-temperature control component; 300-temperature control platform; 400-inert liquid; 101-driving mechanism; 102-spotting gun head; 103-second fixing clamp; 104-piston rod; 105-first fixing clamp; 106-fixing piece; 201-first temperature control unit; 202-second temperature control unit; 203-first rotating seat; 204-temperature storage piece; 2041-first temperature storage block; 2042-second temperature storage block; 205-second rotating seat; 206-base; 301-first semiconductor temperature control board; 302-second semiconductor temperature control board; 303-heat conducting element; 304-temperature regulating component; 305-spotting board; 306-spotting platform; 307-test tube; 308-adapter block; 309-storage platform. DETAILED DESCRIPTION

[0038] The following embodiments of the technical solution of the present application are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0039] See also Figure 1 , Figure 2 and Figure 3 , Figure 1 This is a schematic diagram of the structure of the spotting device in some embodiments of the present application. Figure 2 This is a schematic diagram of the structure of the spotting device 100 according to some embodiments of the present application. Figure 3 The side view of the sample spotting device 100 of some embodiments of the present application is a schematic diagram. The present application provides a sample spotting device, including a temperature control component 200 and a sample spotting device 100, wherein the temperature control component 200 includes a first temperature control component and a temperature storage component 204, wherein the temperature storage component 204 is detachably arranged in the first temperature control component, and the first temperature control component is used to adjust the temperature of the temperature storage component 204; the sample spotting device 100 includes a driving mechanism 101 and a sample spotting head, wherein the sample spotting head is connected to the driving mechanism 101, and the driving mechanism 101 is used to adjust the position of the sample spotting head so that the sample spotting head can be detachably connected to the temperature storage component 204.

[0040] The temperature storage component 204 can be a block. When the temperature storage component 204 is working, the temperature of the temperature storage component 204 itself is adjusted under the action of the first temperature control component. For example, when the spotting material in the spotting head needs to be kept in an environment of about 4°C for a period of time, the temperature of the temperature storage component 204 is adjusted to 4°C through the first temperature control component. Subsequently, the temperature storage component 204 is combined with the spotting head to achieve the purpose of keeping the spotting material in the spotting head warm through the effect of heat conduction.

[0041] The combination of the spotting head and the temperature storage component 204 refers to a heat-conductive connection between the spotting gun tip 102 on the spotting head and the temperature storage component 204 , and the spotting head and the temperature storage component 204 are detachably connected.

[0042] The driving mechanism 101 is used to adjust the position of the sample spotting head so that the sample spotting head can be combined with the temperature storage part 204. Optionally, the driving mechanism 101 can be a three-axis mobile platform. The three-axis mobile platform is disclosed in the prior art. Commonly, the three-axis mobile platform can include a fixed seat, an X-axis moving part, a Y-axis moving part and a Z-axis moving part. The fixed part of the X-axis moving part is connected to the fixed seat, the fixed part of the Y-axis moving part is connected to the movable part of the X-axis moving part, and the fixed part of the Z-axis moving part is connected to the movable part of the Y-axis moving part. In this way, the three-axis mobile platform has a working part that can move in the three directions of X, Y, and Z. In the present application, during the specific implementation, the sample spotting head can be set on the working part of the three-axis mobile platform, so that the sample spotting head can move in the three directions of X, Y, and Z. The three directions of X, Y, and Z can be the directions indicated by the X-axis, Y-axis, and Z-axis in the figure.

[0043] Optionally, the driving mechanism 101 may also be a mechanical arm capable of three-axis movement.

[0044] In the present application, firstly, the spotting head, the temperature storage component 204 and the first temperature control component are independent components, which simplifies the structure of the spotting head and improves the reliability of the spotting head. In addition, by reasonably selecting the material of the temperature storage component 204, the weight of the spotting head and the temperature storage component 204 when they are combined can be further reduced, achieving the purpose of lightweight and improving the moving speed of the spotting head. In addition, the temperature storage component 204 can be a block body without auxiliary components such as pipelines, with a simple structure and reliable operation. It can be sterilized by high temperature and high pressure sterilization or ozone, hydrogen peroxide sterilization and other sterilization methods, which improves the sterilization effect and reduces the risk of contamination of the spotting material.

[0045] See also Figure 2 and Figure 5 , Figure 5 Schematic diagram of the temperature control assembly 200 of some embodiments of the present application. According to some embodiments of the present application, optionally, the temperature storage component 204 includes a first temperature storage block 2041 and a second temperature storage block 2042, and the first temperature storage block 2041 and the second temperature storage block 2042 are magnetically adsorbed to connect the first temperature storage block 2041 and the second temperature storage block 2042 to the spotting head.

[0046] Optionally, the first temperature storage block 2041 and the second temperature storage block 2042 may both be magnetic, or one of the temperature storage blocks may be magnetic and the other may be made of a material capable of magnetic adsorption. The first temperature storage block 2041 and the second temperature storage block 2042 are connected to the sample spotting head, which means that under the action of magnetic adsorption, the first temperature storage block 2041 and the second temperature storage block 2042 are combined with each other, and the sample spotting head is clamped between the first temperature storage block 2041 and the second temperature storage block 2042; or the matching part of the sample spotting head is clamped between the first temperature storage block 2041 and the second temperature storage block 2042.

[0047] Since the first temperature storage block 2041 and the second temperature storage block 2042 are magnetically adsorbed, when the sample spotting head is set between the first temperature storage block 2041 and the second temperature storage block 2042, the first temperature storage block 2041 and the second temperature storage block 2042 can be fixed to the sample spotting head, so that the temperature storage member 204 and the sample spotting head can be detachably connected, and the first temperature storage block 2041 and the second temperature storage block 2042 can be quickly disassembled and assembled. Optionally, the first temperature storage block 2041 and the second temperature storage block 2042 can be respectively provided with notches, and the notches of the two are closed to form a clamping area, and the sample spotting gun head 102 can be set in the clamping area, which increases the contact area between the first temperature storage block 2041 and the second temperature storage block 2042 and the sample spotting head, thereby improving the fixing effect, and also plays a limiting effect, limiting the position of the sample spotting head relative to the first temperature storage block 2041 and the second temperature storage block 2042.

[0048] See also Figure 5 According to some embodiments of the present application, optionally, the first temperature control assembly includes a first temperature control unit 201 and a second temperature control unit 202. The first temperature control unit 201 can be in contact with the first temperature storage block 2041 to reduce the temperature of the first temperature storage block 2041 by heat conduction. Similarly, the second temperature control unit 202 can be in contact with the second temperature storage block 2042 to reduce the temperature of the second temperature storage block 2042 by heat conduction. The time required to adjust the temperature of the two temperature storage blocks is reduced.

[0049] See also Figure 5 According to some embodiments of the present application, optionally, the temperature control assembly 200 further includes a base 206, a first rotating base 203, and a second rotating base 205, wherein the first rotating base 203 and the second rotating base 205 can both rotatably set the base 206; along the first direction, the first temperature control unit 201 and the second temperature control unit 202 are set on the base 206, and the first rotating base 203 and the second rotating base 205 are set between the first temperature control unit 201 and the second temperature control unit 202, and the first rotating base 203 is used to accommodate the two The first rotating seat 203 is used to switch the positions of the two first temperature storage blocks 2041 so that the two first temperature storage blocks 2041 face the first temperature control unit 201 and the second temperature storage block 2042 respectively; the second rotating seat 205 is used to switch the positions of the two second temperature storage blocks 2042 so that the two second temperature storage blocks 2042 face the second temperature control unit 202 and the first temperature storage block 2041 respectively.

[0050] The first direction may be the direction indicated by the X-axis in the figure.

[0051] Since the temperature storage element does not have the function of adjusting its own temperature, the temperature of the temperature storage element will change as the working time increases during the spotting process. This results in a limited working time for a pair of first temperature storage blocks 2041 and second temperature storage blocks 2042. Therefore, on the one hand, the materials of the first temperature storage blocks 2041 and second temperature storage blocks 2042 can be optimized and materials with good heat preservation effects can be selected; on the other hand, by setting multiple groups of first temperature storage blocks 2041 and second temperature storage blocks 2042, when one pair cannot be used, it can be replaced in time.

[0052] The first rotating seat 203 is used to accommodate the two first temperature storage blocks 2041, which means that the first rotating seat 203 is provided with a accommodating component so that the first temperature storage block 2041 can be placed on the first rotating seat 203. The accommodating component can be a groove or a chamber on the first rotating seat 203.

[0053] The second rotating seat 205 is used to accommodate the two second temperature storage blocks 2042, which means that the second rotating seat 205 is provided with a accommodating component so that the second temperature storage block 2042 can be placed on the second rotating seat 205. The accommodating component can be a groove or a chamber on the second rotating seat 205.

[0054] By reasonably setting the positions of the first rotating seat and the second rotating seat and the positions of the first temperature storage block and the second temperature storage block on each rotating seat, it can be achieved that: when the first rotating seat 203 rotates a certain angle, one of the two first temperature storage blocks 2041 faces the first temperature control unit 201, and the other faces the second rotating seat 205; when the second rotating seat 205 rotates a certain angle, one of the two second temperature storage blocks 2042 faces the second temperature control unit 202, and the other faces the first rotating seat 203. The advantage of such a setting is that when the first temperature storage block 2041 and the second temperature storage block 2042 are arranged relative to each other, it is convenient to close and fix the two together on the spotting gun head 102, and at this time, the first temperature storage block 2041 and the second temperature storage block 2042 to be temperature-adjusted just face the corresponding first temperature control unit 201 and the second temperature control unit 202, thereby improving work efficiency.

[0055] Optionally, the first temperature control unit 201, the first rotating seat 203, the second rotating seat 205 and the second temperature control unit 202 can be arranged along a straight line parallel to the first direction, the two first temperature storage blocks 2041 can be arranged opposite to each other on the first rotating seat 203, and the two second temperature storage blocks 2042 can be arranged opposite to each other on the second rotating seat 205.

[0056] Optionally, the first temperature control unit 201 and the second temperature control unit 202 may both include: a semiconductor temperature control component and a heat dissipation component, the refrigeration part of the semiconductor temperature control component abuts against the corresponding temperature storage component to adjust its temperature; the heat dissipation component is connected to the heating part of the semiconductor temperature control component for dissipating heat from the heating part.

[0057] Optionally, the surface of the first temperature storage block 2041 away from the rotation center of the first rotating seat 203 can be an arc surface, so that after the first rotating seat 203 rotates to a certain angle, the first temperature control unit 201 abuts against one of the first temperature storage blocks 2041; the surface of the second temperature storage block 2042 away from the rotation center of the second rotating seat 205 can be an arc surface, so that after the second rotating seat 205 rotates to a certain angle, the second temperature control unit 202 abuts against one of the second temperature storage blocks 2042.

[0058] As mentioned above, since there are two first temperature storage blocks 2041 and second temperature storage blocks 2042, that is, one first temperature storage block 2041 and one second temperature storage block 2042 can form one combination, and another first temperature storage block 2041 and another second temperature storage block 2042 can form another combination. During the use of one combination, the temperature of the other combination can be adjusted by the temperature control component 200. When the temperature of one temperature storage block 2041 and the second temperature storage block 2042 changes during use, they are removed and replaced with another combination, and the cycle is rotated in this way, thereby reducing the influence of the temperature storage components on the working time of the spotting device.

[0059] According to some embodiments of the present application, optionally, along the first direction, the first rotating seat 203 and the second rotating seat 205 are both arranged on the base 206 in an adjustable manner.

[0060] The first rotating seat 203 and the second rotating seat 205 can be adjustably arranged on the base 206 through a sliding component. The sliding component can include a first slide rail and two first sliders. The first slide rail is arranged on the base 206. The two first sliders are slidably connected to the first slide rail. The first rotating seat 203 and the second rotating seat 205 can be rotatably connected to the two first sliders in a one-to-one manner. The first slide rail can extend along the first direction.

[0061] Under the action of the first slide rail and the two first sliders slidably connected to the first slide rail, the first rotating seat 203 and the second rotating seat 205 can translate between the first temperature control unit 201 and the second temperature control unit 202, that is, the first rotating seat 203 can translate with the first temperature storage block 2041 close to the first temperature control unit 201, or translate close to the second temperature storage block 2042, and similarly, the second rotating seat 205 can translate with the second temperature storage block 2042 close to the second temperature control unit 202 or translate close to the first temperature storage block 2041. When the first temperature storage block 2041 and the second temperature storage block 2042 are close to each other, it is convenient to cooperate and install the first temperature storage block 2041 and the second temperature storage block 2042 on the spotting head. When the first temperature storage block 2041 and the second temperature storage block 2042 are translated away from each other and respectively attached to the first temperature control unit 201 and the second temperature control unit 202, the first temperature control unit 201 and the second temperature control unit 202 control the temperature of the first temperature storage block 2041 and the second temperature storage block 2042 respectively.

[0062] Optionally, the spotting head further includes a fixing member 106 to increase the connection strength between the temperature storage member and the spotting head. For example, the fixing member 106 is a fixing sleeve. In a specific implementation, the fixing sleeve is set on the outer peripheral surface of the spotting head, and limiting bosses can be set at both ends of the fixing sleeve to limit the temperature storage member; for another example, the outer peripheral surface of the fixing sleeve can be provided with a magnetic metal layer, and the magnetic metal layer can be made of a metal that can be adsorbed by a magnet. On the one hand, the temperature storage member can be adsorbed on the magnetic layer, which increases the connection strength between the temperature storage member and the spotting head. On the other hand, the metal has good thermal conductivity, so that the temperature of the temperature storage member can be quickly transferred to the spotting gun head.

[0063] See also Figure 2 , Figure 3 and Figure 4 , Figure 4 Schematic diagram of the spotting gun head 102 and the piston rod 104 of some embodiments of the present application. According to some embodiments of the present application, optionally, the spotting head includes the spotting gun head 102 and the piston rod 104, the piston rod 104 is movably disposed in the spotting gun head 102, and the spotting gun head 102 is used to be combined with the temperature storage member 204.

[0064] The piston rod 104 controls the volume of the sample material that can be contained in the sample gun head 102. The liquid outlet of the sample gun head 102 is inserted into the sample material. When the piston rod 104 moves in a direction away from the liquid outlet of the sample gun head 102, the volume of the space containing the sample material in the sample gun head 102 increases, and under the action of atmospheric pressure, the sample material is sucked into the sample gun head 102. On the contrary, when the piston rod 104 moves in a direction close to the liquid outlet of the sample gun head 102, the volume of the space containing the sample material in the sample gun head 102 decreases, and the sample material is pushed out of the sample gun head 102.

[0065] By controlling the position of the piston rod 104 relative to the spotting gun head 102, the volume of the extruded spotting material can be controlled, thereby achieving the purpose of quantitatively spotting the spotting material.

[0066] Since the amount of spotting material in the spotting gun tip 102 is relatively small, the temperature of the spotting material in the spotting gun tip 102 changes rapidly. Therefore, the temperature storage component 204 is matched with the spotting gun tip 102 to avoid denaturation of the spotting material in the spotting gun tip 102 .

[0067] According to some embodiments of the present application, optionally, the spotting device 100 further includes a power mechanism, which is connected to the driving mechanism 101 , and is used to push the piston rod 104 to move relative to the spotting gun head 102 .

[0068] The purpose of driving the piston rod 104 to move relative to the spotting gun head 102 is achieved through the power mechanism, thereby achieving the purpose of drawing in or dripping the spotting material by the spotting head. The power mechanism can be a linear reciprocating moving component. For example, the power mechanism can include a second sliding assembly and a driving member, the second sliding assembly includes a second slide rail and a second slider, the second slide rail is arranged on the working part of the three-axis moving platform, the second slider is slidably connected to the second slide rail, and the second slider is connected to the piston rod 104. When the second slider moves relative to the second slide rail, the piston rod 104 is driven to move relative to the spotting gun head 102.

[0069] Furthermore, the driving member can be a screw rod and a motor. The screw rod is rotatably connected to the second slide rail and is threadedly connected to the second slider. The output shaft of the motor is coaxially connected to the screw rod, so that the motor can drive the screw rod to rotate. When the screw rod rotates, the second slider moves relative to the second slide rail due to the threaded connection between the second slider and the screw rod.

[0070] Optionally, the power mechanism and the piston rod 104 are connected via a second fixing clamp 103 , and the piston rod 104 is clamped in the second fixing clamp 103 .

[0071] See also Figure 6 , Figure 6 Schematic diagram of the temperature control platform 300 structure of some embodiments of the present application. According to some embodiments of the present application, optionally, the spotting device further includes a temperature control platform 300, the temperature control platform 300 includes a third temperature control unit, a fourth temperature control unit, a storage platform 309 and a spotting platform 306, the storage platform 309 is used to store the spotting material, the third temperature control unit is arranged at the bottom of the spotting platform 306, and the fourth temperature control unit is arranged at the bottom of the storage platform 309; wherein the driving mechanism 101 is used to adjust the position of the spotting head so that the spotting head can transfer the spotting material in the storage platform 309 to the spotting platform 306.

[0072] The third temperature control unit is used to adjust the temperature of the spotting platform 306, and the fourth temperature control unit is used to adjust the temperature of the storage platform 309, so that the spotting materials before and after spotting can be in a suitable temperature environment.

[0073] The third temperature control unit is connected to the sample spotting platform 306 by heat conduction, and the fourth temperature control unit is connected to the storage platform 309 by heat conduction.

[0074] The carrier plate disposed on the spotting platform 306 is kept in a set temperature environment by the third temperature control unit, and the spotting material disposed on the storage platform 309 is kept in a set temperature environment by the fourth temperature control unit.

[0075] According to some embodiments of the present application, optionally, the driving mechanism 101 is detachably connected to the spotting head; the storage platform 309 is provided with a placement slot for placing the spotting gun head 102 .

[0076] The driving mechanism 101 is provided with a first fixing clamp 105 , and the spotting gun head 102 is clamped by the first fixing clamp 105 , so that the spotting gun head and the driving mechanism 101 are detachably connected.

[0077] Before using the spotting head, the temperature environment of the spotting head should be set to an environment suitable for storing the spotting material. Otherwise, when the spotting head absorbs the spotting material, the spotting material may be denatured. For example, if the temperature of the spotting material before spotting is 4°C, and the temperature of the inner wall of the spotting head is 10°C, then when the spotting material contacts the inner wall of the spotting head, the spotting material may be denatured. In addition, it takes a long time to adjust the temperature of the spotting head to the set range only through the temperature storage part, which reduces the spotting efficiency.

[0078] In the present application, the spotting head to be used is placed in a placement slot so that the temperature inside the spotting head is suitable for placing the spotting material, thereby avoiding denaturation of the spotting material due to inappropriate temperature when the spotting material is drawn into the spotting head, and avoiding the influence of the heating or cooling process on the performance of the spotting material or cell activity.

[0079] Optionally, the storage platform 309 may also be provided with a test tube 307 slot for placing the test tube 307 , and the test tube 307 may contain materials to be spotted.

[0080] Optionally, the spotting platform 306 may further include a loading slot for placing the spotting plate 305 , on which the spotting material is spotted.

[0081] The placement slots, test tube 307 slots and object loading slots play a fixing role on the one hand, and on the other hand, the inner wall of each slot is arranged around the outer wall of the object placed therein, thereby improving the temperature adjustment effect.

[0082] Optionally, the spotting platform 306 and the storage platform 309 are connected by a transfer block 308, and the transfer block 308 makes the two combined as a whole, and is easy to move while being installed and arranged. In the present application, the thermal conductivity of the transfer block 308 is less than or equal to 1w / mk, which reduces the heat conduction between the spotting platform 306 and the storage platform 309. For example, the transfer block 308 can be mica or ABS plastic.

[0083] See also Figure 6 According to some embodiments of the present application, optionally, the third temperature control unit includes a first semiconductor temperature control board 301, the fourth temperature control unit includes a second semiconductor temperature control board 302, the first semiconductor temperature control board 301 includes a first working surface and a first non-working surface that are oppositely arranged, and the second semiconductor temperature control board 302 includes a second working surface and a second non-working surface that are oppositely arranged;

[0084] The first working surface of the first semiconductor temperature control plate 301 abuts against the spotting platform 306 , the second working surface of the second semiconductor temperature control plate 302 abuts against the storage platform 309 , and the first non-working surface of the first semiconductor temperature control plate 301 and the second non-working surface of the second semiconductor temperature control plate 302 are connected via a heat conducting element 303 .

[0085] The first working surface of the first semiconductor temperature control board 301 is one of the heating surface and the cooling surface of the first semiconductor temperature control board 301, and the first non-working surface is the other of the heating surface and the cooling surface of the first semiconductor temperature control board 301. The second working surface of the second semiconductor temperature control board 302 is one of the heating surface and the cooling surface of the second semiconductor temperature control board 302, and the second non-working surface is the other of the heating surface and the cooling surface of the second semiconductor temperature control board 302.

[0086] The two semiconductor temperature control boards are disclosed in the prior art, and their detailed structures and working principles are not described here in detail.

[0087] The first non-working surface of the first semiconductor temperature control board 301 and the second non-working surface of the second semiconductor temperature control board 302 are connected by the heat conducting element 303, so that the non-working surfaces of the two are connected by heat conduction. If one of the working surfaces of the two semiconductor temperature control boards is for cooling and the other is for heating, at this time, one of the two non-working surfaces is for cooling and the other is for heating. The heat conducting element 303 neutralizes and balances the temperatures of the two non-working surfaces, thereby improving the working efficiency of the working surfaces of the two semiconductor temperature control boards; if the working surfaces of the two semiconductor temperature control boards are both for cooling, then the working surfaces of the two semiconductor temperature control boards are both for heating. The heat dissipation area of ​​the two working surfaces is increased by the heat conducting element 303, the heat dissipation effect is increased, and the working efficiency of the working surfaces of the two semiconductor temperature control boards is improved.

[0088] Furthermore, the temperature control platform 300 may also include a temperature adjustment component 304, which is used to adjust the temperature of the heat-conducting element 303. For example, if the working surfaces of the two semiconductor temperature control boards are both cooling, the heat dissipation effect of the heat-conducting element 303 can be reduced by the temperature adjustment component 304 to improve the cooling efficiency of the two working surfaces. If the working surfaces of the two semiconductor temperature control boards are both heating, the temperature of the heat-conducting element 303 can be increased by the temperature adjustment component 304, the temperature of the non-working surfaces of the two semiconductor temperature control boards can be reduced, and the heating effect of the working surfaces of the two semiconductor temperature control boards can be improved.

[0089] The temperature adjustment component 304 may have both cooling and heating functions, or may be provided with two, one of which has a heating function and the other has a cooling function.

[0090] In one embodiment, the second working surface of the second semiconductor temperature control plate 302 is cooled, and the first working surface of the first semiconductor temperature control plate 301 is heated. The heat generated by the second non-working surface of the second semiconductor temperature control plate 302 can be transferred to the first non-working surface of the first semiconductor temperature control plate 301 for heating through the heat conducting element 303, and with the assistance of the temperature regulating component 304, the spotting platform 306 and the storage platform 309 of the temperature control platform are ensured to be maintained at the required temperature. This design can not only save energy to the maximum extent, but also minimize the disturbance to the experimental environment by dissipating heat through the heat conducting element 303, and maintain the stability of the environment required during the spotting process.

[0091] Optionally, the thermal conductivity of the spotting platform 306 and the storage platform 309 is greater than or equal to 200 w / mk. For example, the spotting platform 306 and the storage platform 309 can be made of metal copper or metal aluminum.

[0092] The method for using the device comprises the following steps: S1, pre-temperature adjustment of the sample spotting gun head 102 through the storage platform 309, pre-temperature adjustment of the test tube 307 and the materials to be used in the test tube 307 through the storage platform 309, and pre-temperature adjustment of the sample spotting plate 305 through the sample spotting platform 306; S2, inert liquid 400 is pre-absorbed into the sample spotting gun head 102; S3, the sample spotting head moves to the storage platform 309, and the sample spotting gun head 102 with the inert liquid 400 is grasped by the first fixing clamp 105; S4, the sample spotting gun head 102 absorbs the sample spotting material on the storage platform 309; S5, the sample spotting head moves to the temperature control component 200, and the first temperature storage block 2041 and the second temperature storage block 2042 are installed on the sample spotting head; S6, the sample spotting head moves to the sample spotting platform 306 for sample spotting.

[0093] The purpose of step S1 is to make the temperature environment of the sample spotting gun head 102 and the material in the test tube 307 suitable for storing the sample spotting material before using the sample spotting gun head 102, so as to avoid denaturation of the sample spotting material when the sample spotting material is first sucked in. The temperature of the sample spotting plate 305 is a temperature environment suitable for completing the curing of the sample spotting material.

[0094] In step S2, when the gas in the spotting gun head 102 is compressed by the piston rod 104 to discharge the spotting material, the gas can be compressed, resulting in an error in the volume of the discharged spotting material. By sucking the inert liquid 400 into the spotting gun head 102 in advance, the spotting gun head 102 does not have air, which reduces the error when the piston rod 104 pushes out the spotting material. The inert liquid 400 can be gelatin, glycerol and sugar. The prefabricated material used must ensure that it can be layered with the spotting material or the piston of the spotting gun head 102 is designed in a special shape to achieve extremely low residual air inside the spotting gun head 102. When the spotting material is blown and premixed, there can be no air pockets, the microspheres produced have no bubbles, and the high-viscosity materials sucked are also more accurate.

[0095] In step S3, the spotting head grabs the spotting gun tip 102 through the first fixing clamp 105, and the spotting gun tip can be quickly grabbed and detached, so that different spotting gun tips can be quickly switched during the spotting process.

[0096] In step S4, the spotting material to be sucked by the spotting head is stored in the test tube 307, and the test tube 307 is set on the storage platform 309. Under the action of the second semiconductor temperature control plate 302, the spotting material in the test tube 307 can be kept in a suitable temperature environment to avoid denaturation of the spotting material. In step S5, when the spotting head is combined with the first temperature storage block 2041 and the second temperature storage block 2042, the spotting head can be moved between the first rotating seat 203 and the second rotating seat 205, and then a first temperature storage block 2041 and a second temperature storage block 2042 are closed; before step S2, the following steps are also included: the spotting gun head 102 of the spotting head is clamped in the first fixing clamp 105, and the piston rod 104 of the spotting head is clamped in the second fixing clamp 103, so that the power mechanism drives the piston rod 104 to move, and in subsequent steps, the spotting head is moved by the driving mechanism 101, and the piston rod 104 is controlled to move by the power mechanism.

[0097] In step S6 , the spotting plate 305 is placed on the spotting platform 306 , and the spotting head drops the spotting material onto the spotting plate 305 .

[0098] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them; although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should all be included in the scope of the claims and specification of the present application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions that fall within the scope of the claims.

Claims

1. A spotting device, characterized in that: include: A temperature control component, comprising a first temperature control component and a temperature storage component, wherein the temperature storage component is detachably arranged on the first temperature control component, and the first temperature control component is used to adjust the temperature of the temperature storage component; The sample spotting device comprises a driving mechanism and a sample spotting head, wherein the sample spotting head is connected to the driving mechanism, and the driving mechanism is used to adjust the position of the sample spotting head so that the sample spotting head can be detachably connected to the temperature storage component; The temperature storage component includes a first temperature storage block and a second temperature storage block, and the first temperature storage block and the second temperature storage block are detachably connected so that the first temperature storage block and the second temperature storage block are connected to the spotting head; The first temperature control assembly includes a first temperature control unit and a second temperature control unit, and the first temperature control unit and the second temperature control unit are used to adjust the temperatures of the first temperature storage block and the second temperature storage block respectively; The temperature control assembly further comprises a base, a first rotating base, and a second rotating base, wherein the first rotating base and the second rotating base can both rotatably set the base; Along the first direction, the first temperature control unit and the second temperature control unit are arranged on the base, the first rotating seat and the second rotating seat are arranged between the first temperature control unit and the second temperature control unit, the first rotating seat is used to accommodate two of the first temperature storage blocks, and the second rotating seat is used to accommodate two of the second temperature storage blocks; Wherein, the first rotating seat is used to switch the positions of the two first temperature storage blocks so that the two first temperature storage blocks face the first temperature control unit and one second temperature storage block respectively; the second rotating seat is used to switch the positions of the two second temperature storage blocks so that the two second temperature storage blocks face the second temperature control unit and one first temperature storage block respectively; The spotting device further comprises a temperature control platform, the temperature control platform comprises a third temperature control unit, a fourth temperature control unit, a storage platform and a spotting platform, the storage platform is used to store the spotting material, the third temperature control unit is arranged at the bottom of the spotting platform, and the fourth temperature control unit is arranged at the bottom of the storage platform; wherein the driving mechanism is used to adjust the position of the spotting head so that the spotting head can transfer the spotting material in the storage platform to the spotting platform; The third temperature control unit includes a first semiconductor temperature control board, and the fourth temperature control unit includes a second semiconductor temperature control board; The first semiconductor temperature control plate comprises a first working surface and a first non-working surface which are arranged opposite to each other, and the second semiconductor temperature control plate comprises a second working surface and a second non-working surface which are arranged opposite to each other; The first working surface of the first semiconductor temperature control plate abuts against the spotting platform, the second working surface of the second semiconductor temperature control plate abuts against the storage platform, and the first non-working surface of the first semiconductor temperature control plate and the second non-working surface of the second semiconductor temperature control plate are connected through a heat conducting element.

2. The spotting device according to claim 1, characterized in that: Along the first direction, the first rotating seat and the second rotating seat are both arranged on the base in an adjustable manner.

3. The spotting device according to claim 1, characterized in that: The sample spotting head comprises a sample spotting gun head and a piston rod. The piston rod is movably arranged in the sample spotting gun head. The sample spotting gun head is used to be combined with the temperature storage component.

4. The spotting device according to claim 3, characterized in that: The sample spotting device further comprises a power mechanism, which is connected to the driving mechanism and is used to push the piston rod to move relative to the sample spotting gun head.

5. The spotting device according to claim 1, characterized in that: The driving mechanism is detachably connected to the spotting head; and the storage platform is provided with a placement slot for placing the spotting gun head.

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