Hemoglobin concentration measurement method, system and intelligent device

Abstract

The present invention discloses a hemoglobin concentration measurement method, system and intelligent device, the method comprising: performing a transmittance test on a counting plate with a hemoglobin sample placed thereon, obtaining a set of test data points, establishing a curve graph corresponding to a displacement-light intensity relationship based on the set of test data points; obtaining a set of test data points corresponding to the stage when the counting plate moves to an air chamber position; obtaining a set of test data points corresponding to the stage when the hemoglobin chamber position is in place; obtaining the mean light intensity in the air chamber stage and the mean light intensity in the hemoglobin chamber position stage; obtaining the hemoglobin concentration of the hemoglobin solution to be measured. The present invention obtains the mean light intensity in the air chamber stage and the mean light intensity in the hemoglobin chamber position stage in the curve graph corresponding to the displacement-light intensity relationship, and finally quickly and accurately obtains the detection result of the hemoglobin concentration, thereby avoiding the problem of deviation in the measurement result caused by the inability of the counting plate or the measuring component to move to the desired position.

Description

Technical Field

[0001] The present invention relates to the field of concentration measurement technology, and in particular to a hemoglobin concentration measurement method, system and intelligent device. Background Art

[0002] During the hemoglobin concentration measurement process, it is necessary to test the light projection effect at different positions of the blood to be tested. At this time, the counting board or the test component needs to be moved. Since the counting board or the test component may have large errors during the movement, such as step loss, the counting board or the measuring component may not be able to move to the desired position, resulting in deviations in the final hemoglobin measurement results.

[0003] Therefore, it is urgent to propose a hemoglobin concentration measurement method to solve the problem raised. Summary of the invention

[0004] Based on this, it is necessary to provide a hemoglobin concentration measurement method, system and intelligent device to address the deficiencies of the existing technology, which can quickly and accurately detect the hemoglobin concentration results.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] In a first aspect, a hemoglobin concentration measurement method is provided, which comprises the following steps: Step S110, performing a transmittance test on a counting plate on which a hemoglobin sample is placed, and obtaining a test data point set , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set;

[0007] Step S120, obtaining a set of test data points corresponding to the stage when the counting plate moves to the air chamber position in the curve diagram corresponding to the displacement-light intensity relationship;

[0008] Step S130, obtaining a set of experimental data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship;

[0009] Step S140: Obtain the light intensity mean value of the air cavity stage based on the experimental data point set corresponding to the air cavity position stage obtained in step S120. , based on the experimental data point set corresponding to the hemoglobin cavity position stage obtained in step S130, the light intensity mean value of the hemoglobin cavity position stage is obtained. ;

[0010] Step S150: Based on the Beer-Lambert law formula and the light intensity mean , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When is the hemoglobin concentration value of a known hemoglobin solution, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0011] In a second aspect, a hemoglobin concentration measurement system is provided, comprising:

[0012] The acquisition module is used to perform a transmittance test on the counting plate with the hemoglobin sample placed on it and obtain a set of test data points. , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set;

[0013] The first acquisition module is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the air chamber position in a curve diagram corresponding to the displacement-light intensity relationship;

[0014] The second acquisition module is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship;

[0015] The first calculation module is used to obtain the experimental data point set corresponding to the air cavity position stage to obtain the light intensity mean of the air cavity stage The light intensity mean of the hemoglobin cavity position stage is obtained by using the experimental data point set corresponding to the hemoglobin cavity position stage ;

[0016] The second calculation module is used based on the formula of Lambert-Beer law and the mean light intensity , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When is the hemoglobin concentration value of a known hemoglobin solution, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0017] In a third aspect, an intelligent device is provided, which includes a memory and a processor, wherein a computer program is stored in the memory, and the processor implements the above-mentioned hemoglobin concentration measurement method when executing the computer program.

[0018] In summary, the hemoglobin concentration measurement method, system and intelligent device of the present invention obtains the experimental data point set corresponding to the air cavity position stage and the experimental data point set corresponding to the hemoglobin cavity position stage in the curve diagram corresponding to the displacement-light intensity relationship, and then obtains the light intensity mean value of the air cavity stage. and the average light intensity at the hemoglobin cavity position Finally, based on the Beer-Lambert law formula, the hemoglobin concentration test result is quickly and accurately obtained, thereby avoiding the problem that the counting plate or the measuring component cannot be moved to the desired position, resulting in deviation in the final hemoglobin measurement result. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a structural schematic diagram of a concentration measurement device provided by an embodiment of the present invention;

[0020] Figure 2 is a flow chart of a hemoglobin concentration measurement method provided by an embodiment of the present invention;

[0021] Figure 3 A curve graph corresponding to the relationship between displacement and light intensity established based on a test data point set provided in an embodiment of the present invention;

[0022] Figure 4 It is a curve diagram corresponding to the displacement-light intensity relationship formed by combining displacement-light intensity curves corresponding to multiple position stages during the relative movement of the counting plate and the measuring component provided by the embodiment of the present invention;

[0023] Figure 5 A structural block diagram of a hemoglobin concentration measurement system provided by an embodiment of the present invention;

[0024] Figure 6 A structural block diagram of an intelligent device provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0025] In order to further understand the features, technical means, specific objectives and functions of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0026] Figure 1 FIG. 1 is a schematic diagram showing a hemoglobin concentration measurement method and system provided by an embodiment of the present invention, and a concentration measurement device applicable to an intelligent device. Figure 1As shown, the hemoglobin concentration measurement system includes a counting plate 100 and a measuring component 200, wherein the counting plate 100 is provided with a plurality of cavities 101, and two adjacent cavities are separated by a transparent partition 102, wherein one of the cavities 101 contains a hemoglobin sample to be detected, which is recorded as a hemoglobin solution cavity, wherein the other cavity 101 is empty, which is recorded as an air cavity, the partition 102 between the hemoglobin cavity and the air cavity is a second partition, and the partition 102 on the other side of the hemoglobin cavity is recorded as a first partition; the counting plate 100 and the measuring component 200 can be relatively moved, from It is convenient to measure the transmittance of each cavity position on the counting board 100 respectively; the measuring component 200 includes an emitter 210 and a photoelectric conversion module 220, and the emitter 210 and the photoelectric conversion module 220 are respectively arranged on both sides of the counting board 100, for testing the transmittance of different positions of the counting board 100; in this embodiment, the emitter 210 refers to a light emitting diode, and the photoelectric conversion module 220 includes a photodiode, an amplifying circuit and an analog-to-digital converter, etc., for receiving a light signal and sending the light intensity value of the light signal to a control unit, which is a known technology and need not be elaborated here.

[0027] Specifically, the emitter 210 is fixedly arranged in front of the counting plate 100, the photoelectric conversion module 220 is fixedly arranged behind the counting plate 100, and a movable slide rail 300 is arranged under the counting plate 100. The counting plate 100 moves along the direction of the movable slide rail 300, thereby facilitating the measuring component 200 to measure the transmittance of different positions of the counting plate 100.

[0028] Alternatively, a first guide rail parallel to the counting board 100 is provided in front of the counting board 100, a second guide rail parallel to the counting board 100 is provided behind the counting board 100, a first transfer seat is provided on the first guide rail, the emitter 210 is provided on the first transfer seat, a second transfer seat is provided on the second guide rail, and the photoelectric conversion module 220 is provided on the second transfer seat. The first transfer seat and the second transfer seat are driven by an external driving device and move synchronously along the first guide rail and the second guide rail respectively, thereby achieving the purpose of measuring the transmittance of each cavity on the counting board 100.

[0029] In this embodiment, the emitter 210 emits an initial light, which is received by the photoelectric conversion module 220 after passing through the counting plate. The photoelectric conversion module 220 receives the light and sends the light intensity value to the control unit. Before testing the hemoglobin sample to be tested contained in the cavity of the counting plate 100, a hemoglobin solution corresponding to at least one known hemoglobin concentration value is measured; under the condition that the light intensity of the light emitted by the emitter 210 remains unchanged, the relationship between the hemoglobin concentration and the light intensity value of the light received by the photoelectric conversion module 220 is obtained.

[0030] Specifically, measuring the concentration of hemoglobin solution (HGB) is actually measuring the degree of light absorption of the hemoglobin solution, that is, the absorption coefficient. Under the condition of a certain light intensity of the light-emitting diode, the higher the HGB concentration value, the greater the absorption coefficient, and the smaller the light intensity value received by the photoelectric conversion module.

[0031] Set the known hemoglobin solution concentration to When , the light intensity of the light received by the photoelectric conversion module 220 after the initial light emitted by the emitter 210 passes through the air cavity of the counting plate is I, and the light intensity of the light received by the photoelectric conversion module 220 after the initial light emitted by the emitter 210 passes through the known hemoglobin solution cavity of the counting plate is I0, wherein, multiple light intensity values ​​of the incident light can be obtained by measuring the light emitted by the emitter through the air cavity of the counting plate by multiple or multi-point methods, and finally the light intensity I of the light received by the photoelectric conversion module 220 is obtained based on the multiple light intensity values ​​of the incident light; multiple or multi-point methods can be used to measure the light emitted by the emitter through the known hemoglobin solution cavity of the counting plate to obtain multiple light intensity values, and finally the light intensity I0 of the light received by the photoelectric conversion module 220 is obtained based on the multiple light intensity values ​​of the incident light, the multi-point method refers to the same initial light passing through different positions of the counting plate cavity, and the multiple method refers to the same initial light passing through the same position of the counting plate cavity multiple times.

[0032] According to the "Lambert-Beer Law" formula: , where A is the absorbance and T is the transmittance (transmittance), which is characterized by the light intensity I of the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate and the light intensity I of the photoelectric conversion module after the initial light emitted by the emitter passes through the hemoglobin solution cavity to be tested in the counting plate x The ratio of T = I / I x , b is the thickness of the absorption layer, is the concentration of the absorbing substance, K is the molar absorption coefficient, which is related to the properties of the absorbing substance and the wavelength λ of the incident light. The properties of the absorbing substance in hemoglobin solutions with different concentrations are the same. Therefore, in the present invention, the absorbing substance refers to hemoglobin, K is a fixed value, and the size of the counting plate cavity is fixed, and b is a fixed value. In this embodiment, when the hemoglobin concentration is known to be , T=I / I0.

[0033] Since K and b are fixed values, the formula can be Consider A A linear function, due to , so we know .

[0034] The hemoglobin concentration of the hemoglobin solution to be tested is The light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter 210 passes through the air chamber of the counting plate is I, and the light intensity of the light received by the photoelectric conversion module 220 after the initial light emitted by the emitter 210 passes through the hemoglobin solution chamber to be tested of the counting plate is I x When, based on the formula , and T=I / I x The hemoglobin concentration of the hemoglobin solution to be tested is obtained as .

[0035] Figure 2 is a flow chart of a first hemoglobin concentration measurement method provided by an embodiment of the present invention, such as Figure 2 As shown, the hemoglobin concentration measurement method includes steps S110 to S140, which are specifically as follows:

[0036] Step S110: Perform a transmittance test on the counting plate with the hemoglobin sample placed thereon to obtain a test data point set. , based on the experimental data point set, a curve graph corresponding to the displacement-light intensity relationship is established, such as Figure 3 As shown; where i∈[1, n], in the initial state, the emitter and the photoelectric conversion module are both located on the outer side of the counting board.

[0037] The method of step S110 is specifically performed as follows:

[0038] The external driving device drives the counting board to move different displacements along the direction of the moving slide rail, and the light signal emitted by the transmitter is received by the photoelectric conversion module, so as to obtain the light intensity received by the photoelectric conversion module corresponding to the different displacements of the counting board; as the counting board moves, the light signal emitted by the transmitter will be directly received by the photoelectric conversion module through the air, and then through the cavity of the counting board and then received by the photoelectric conversion module, so that the photoelectric conversion module obtains the light intensity corresponding to the movement of the counting board to different positions;

[0039] Obtain the displacement-light intensity curves corresponding to the movement of the counting plate to multiple position stages in the curve graph corresponding to the displacement-light intensity relationship, and the displacement-light intensity curves corresponding to the multiple position stages are combined into a curve graph corresponding to the displacement-light intensity relationship, such as Figure 4 As shown; wherein, the multiple position stages include an air position stage, a first partition position stage, an air cavity position stage, a second partition position stage, and a hemoglobin cavity position stage. The air position stage refers to the presence of only air medium between the emitter and the photoelectric conversion module, and the measuring component and the counting board are in a staggered setting state.

[0040] Step S120, obtaining a set of experimental data points corresponding to the stage when the counting plate moves to the air chamber position in the curve diagram corresponding to the displacement-light intensity relationship.

[0041] Specifically, the method of step S120 includes the following steps:

[0042] Obtain the light intensity values ​​of the test data points corresponding to multiple peaks and multiple troughs in the curve graph corresponding to the displacement-light intensity relationship; specifically, use the scipy.signal.find_peaks() method in Python to obtain the peaks and troughs in the curve graph corresponding to the displacement-light intensity relationship, and then obtain the light intensity value of the test data point corresponding to each peak and the light intensity value of the test data point corresponding to each trough;

[0043] In the curve diagram corresponding to the displacement-light intensity relationship, obtain the test data point corresponding to the peak corresponding to the maximum light intensity value, which is recorded as the first test data point; the peak with the maximum light intensity value is the peak value of the displacement-light intensity curve corresponding to the second partition position stage;

[0044] In the curve graph corresponding to the displacement-light intensity relationship, the test data point corresponding to the second trough close to the first test data point is obtained, which is recorded as the second test data point. The second test data point is one of the test data points in the air chamber position stage; the test data points corresponding to the troughs in the curve graph corresponding to the displacement-light intensity relationship are traversed, and the x-axis coordinate value x of the test data point corresponding to each trough in the curve graph corresponding to the displacement-light intensity relationship is obtained. 01 、x 02 …x 0（m-1） 、x 0m and the x-axis coordinate value x of the test data point corresponding to the peak with the largest intensity value t , where m is the number of valleys in the curve corresponding to the displacement-light intensity relationship, then x 0（m-1） is the x-axis coordinate value of the test data point corresponding to the second trough close to the peak with the maximum light intensity value in the curve graph corresponding to the displacement-light intensity relationship, recorded as x k , then x k =x 0（m-1） ;

[0045] The first k test data points and the last k test data points close to the second test data point in the curve graph corresponding to the displacement-light intensity relationship are obtained, that is, other test data points corresponding to the air chamber position stage in the displacement-light intensity curve, so as to obtain the test data point set corresponding to the counting plate moving to the air chamber position stage. The test data point set corresponding to the air chamber position stage includes the first test data point, the first k test data points and the last k test data points close to the second test data point in the curve graph corresponding to the displacement-light intensity relationship; wherein the maximum value of k can be ⌊ ⌋, where d refers to the displacement of the counting plate when the measuring component collects adjacent test data points, L refers to the preset length of the air chamber stage on the counting plate, in this embodiment, k is 5, and the coordinates corresponding to the first k test data points and the last k test data points close to the second test data point and the second test data point are (x0, y0), (x1, y1)…(x k-1 ,y k-1 ), (x k ,y k ), (x k+1 ,y k+1 ), (x k+2 ,y k+2 )…(x 2k ,y 2k ).

[0046] For example, by measuring the length of the air cavity of the counting plate, it can be known that the length of the air cavity position is 4mm=4000μm, and the displacement of the counting plate during two adjacent acquisitions of the measuring component is 125μm. The corresponding test data points in the air cavity position stage are approximately 4000÷125=32. When the first test data point is obtained, up to 15 test data points before and after the first test data point can be collected as the test data point set corresponding to the air cavity position stage as needed.

[0047] Step S130, obtaining a set of experimental data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship.

[0048] Specifically, the method of step S130 includes the following steps:

[0049] Obtain the light intensity values ​​of the test data points corresponding to multiple peaks and multiple troughs in the curve graph corresponding to the displacement-light intensity relationship; specifically, use the scipy.signal.find_peaks() method in Python to obtain the peaks and troughs in the curve graph corresponding to the displacement-light intensity relationship, and then obtain the light intensity value of the test data point corresponding to each peak and the light intensity value of the test data point corresponding to each trough;

[0050] In the curve diagram corresponding to the displacement-light intensity relationship, obtain the test data point corresponding to the peak corresponding to the maximum light intensity value, which is recorded as the first test data point; the peak with the maximum light intensity value is the peak value of the displacement-light intensity curve corresponding to the second partition position stage;

[0051] In the curve graph corresponding to the displacement-light intensity relationship, obtain a point whose x-axis coordinate value is greater than the x-axis coordinate value x of the first test data point. tThe corresponding y-axis coordinate values ​​of the multiple test data points are recorded as y1, y2…y n-1 ,y n , the y-axis coordinate value of the first test data point is y t ;

[0052] Based on y n -y n-1 ≤△T to obtain the test data point set corresponding to the stage when the counting plate moves to the hemoglobin holding cavity position, wherein △T is a preset threshold value; in the curve diagram corresponding to the displacement-light intensity relationship, the test data point whose x-axis coordinate value is greater than the x-axis coordinate value of the first test data point is used as a reference, recorded as the third test data point set, the third test data point set is traversed, and the test data point set corresponding to the hemoglobin holding cavity position stage is obtained from the third test data point set, recorded as the fourth test data point set, and the test data points in the fourth test data point set meet the requirement that the y-axis coordinate value of the current test data point minus the y-axis coordinate value of the previous test data point is less than the preset threshold value △T.

[0053] Step S140: Obtain the light intensity mean value of the air cavity stage based on the experimental data point set corresponding to the air cavity position stage obtained in step S120. , based on the experimental data point set corresponding to the hemoglobin cavity position stage obtained in step S130, the light intensity mean value of the hemoglobin cavity position stage is obtained. In this embodiment, , .

[0054] Step S150: Based on the Beer-Lambert law formula and the light intensity mean , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When is the hemoglobin concentration value of the known hemoglobin solution, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate; When is the hemoglobin concentration of the hemoglobin solution to be tested, Refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the transmitter passes through the hemoglobin solution chamber to be tested in the counting plate. Refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the transmitter passes through the air cavity of the counting plate.

[0055] Specifically, according to the "Lambert-Beer Law" formula: , where A is absorbance and T is transmittance (transmittance). When the hemoglobin concentration of the hemoglobin solution to be measured is , T represents the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate. The light intensity of the light received by the photoelectric conversion module after the initial light emitted by the transmitter passes through the hemoglobin solution chamber to be tested in the counting plate The ratio of , b is the thickness of the absorption layer, is the concentration of the absorbing substance, K is the molar absorption coefficient, which is related to the properties of the absorbing substance and the wavelength λ of the incident light. The properties of the absorbing substance in hemoglobin solutions with different concentrations are the same. Therefore, in the present invention, the absorbing substance refers to hemoglobin, K is a fixed value, and the size of the counting plate cavity remains unchanged, and b is a fixed value. Since k and b are fixed values, the formula can be Consider A A linear function, due to , so we know , and finally through the formula The hemoglobin concentration of the hemoglobin solution to be tested can be calculated .

[0056] The hemoglobin concentration measurement method of the present invention obtains the experimental data point set corresponding to the air cavity position stage and the experimental data point set corresponding to the hemoglobin cavity position stage in the curve diagram corresponding to the displacement-light intensity relationship, and then obtains the light intensity mean value of the air cavity stage. and the average light intensity at the hemoglobin cavity position Finally, based on the Beer-Lambert law formula, the hemoglobin concentration test result is quickly and accurately obtained, thereby avoiding the problem that the counting plate or the measuring component cannot be moved to the desired position, resulting in deviation in the final hemoglobin measurement result.

[0057] Figure 5 is a structural block diagram of a first hemoglobin concentration measurement system provided by an embodiment of the present invention, such as Figure 5 As shown, corresponding to the above-mentioned hemoglobin concentration measurement method, the present invention further provides a hemoglobin concentration measurement system, the hemoglobin concentration measurement system includes a module for executing the above-mentioned hemoglobin concentration measurement method, the system can be configured in a terminal such as a smart device, and the hemoglobin concentration measurement system of the present invention is applied to obtain a test data point set corresponding to the air cavity position stage and a test data point set corresponding to the hemoglobin cavity position stage in a curve diagram corresponding to the displacement-light intensity relationship, thereby obtaining the light intensity mean value of the air cavity stage. and the average light intensity at the hemoglobin cavity position Finally, based on the Beer-Lambert law formula, the hemoglobin concentration test result is quickly and accurately obtained, thereby avoiding the problem that the counting plate or the measuring component cannot be moved to the desired position, resulting in deviation in the final hemoglobin measurement result.

[0058] Specifically, Figure 5 As shown, the hemoglobin concentration measurement system includes a collection module 110 , a first acquisition module 120 , a second acquisition module 130 , a first calculation module 140 and a second calculation module 150 .

[0059] The acquisition module 110 is used to perform a transmittance test on the counting plate on which the hemoglobin sample is placed, and obtain a set of test data points. , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set;

[0060] The first acquisition module 120 is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the air chamber position in the curve diagram corresponding to the displacement-light intensity relationship;

[0061] The second acquisition module 130 is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship;

[0062] The first calculation module 140 is used to obtain the experimental data point set corresponding to the air cavity position stage to obtain the light intensity mean value of the air cavity stage The light intensity mean of the hemoglobin cavity position stage is obtained by using the experimental data point set corresponding to the hemoglobin cavity position stage ;

[0063] The second calculation module 150 is used to calculate the light intensity based on the formula of Lambert-Beer law and the light intensity mean value. , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When the concentration of the hemoglobin solution is known, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0064] The hemoglobin concentration measurement system of the present invention obtains the experimental data point set corresponding to the air cavity position stage and the experimental data point set corresponding to the hemoglobin cavity position stage in the curve diagram corresponding to the displacement-light intensity relationship, thereby obtaining the light intensity mean value of the air cavity stage. and the average light intensity at the hemoglobin cavity position Finally, based on the Beer-Lambert law formula, the hemoglobin concentration test result is quickly and accurately obtained, thereby avoiding the problem that the counting plate or the measuring component cannot be moved to the desired position, resulting in deviation in the final hemoglobin measurement result.

[0065] It should be noted that those skilled in the art can clearly understand that the specific implementation process of the above-mentioned hemoglobin concentration measurement system and each module can refer to the corresponding description in the aforementioned method embodiment, and for the convenience and brevity of description, it will not be repeated here.

[0066] Figure 6 is a block diagram of the internal structure of an intelligent device provided by an embodiment of the present invention, such as Figure 6 As shown, the smart device provided by the present invention includes a memory, a processor and a network interface connected through a system bus; a computer program is stored in the memory, and the processor is used to provide computing and control capabilities to support the operation of the entire smart device. When the processor executes the computer program, the above-mentioned hemoglobin concentration measurement method is implemented.

[0067] The memory may include a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and may also store a computer program. When the computer program is executed by the processor, the processor may implement the hemoglobin concentration measurement method.

[0068] The internal memory may also store a computer program, which, when executed by the processor, enables the processor to execute the hemoglobin concentration measurement method. The network interface is used to communicate with other devices over the network. Those skilled in the art will appreciate that Figure 6 The structure shown in the figure is only a block diagram of a partial structure related to the solution of the present application, and does not constitute a limitation on the application of the solution of the present application to other smart devices. A specific smart device may include more or fewer components than those shown in the figure, or combine certain components, or have a different arrangement of components.

[0069] In one embodiment, the hemoglobin concentration measurement method provided in the present application can be implemented as a computer program. The computer program can be Figure 6 The memory of the smart device can store various program modules that constitute the hemoglobin concentration measurement system, such as: Figure 5 The acquisition module 110, the first acquisition module 120, the second acquisition module 130, the first calculation module 140 and the second calculation module 150 shown. The computer program composed of each program module enables the processor to execute the steps of the hemoglobin concentration measurement system of each embodiment of the present application described in this specification. For example, Figure 6 The smart device shown can be Figure 5 The acquisition module 110 in the hemoglobin concentration measurement system shown in the figure performs a transmittance test on the counting plate on which the hemoglobin sample is placed, and obtains a set of test data points. , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set; the first acquisition module 120 obtains the experimental data point set corresponding to the stage when the counting plate moves to the air chamber position in the curve graph corresponding to the displacement-light intensity relationship; the second acquisition module 130 obtains the experimental data point set corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship; the first calculation module 140 obtains the experimental data point set corresponding to the air chamber position stage to obtain the light intensity mean of the air chamber stage The light intensity mean of the hemoglobin cavity position stage is obtained by using the experimental data point set corresponding to the hemoglobin cavity position stage The second calculation module 150 is based on the Lambert-Beer law formula and the light intensity mean , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When the concentration of the hemoglobin solution is known, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0070] In one embodiment, a smart device is provided, including a memory and a processor, wherein the memory stores a computer program, and when the computer program is executed by the processor, the processor performs the following steps: Step S110, performing a transmittance test on a counting plate with a hemoglobin sample placed thereon, and obtaining a set of test data points. , establish a curve graph corresponding to the displacement-light intensity relationship based on the experimental data point set; step S120, obtain the experimental data point set corresponding to the stage when the counting plate moves to the air chamber position in the curve graph corresponding to the displacement-light intensity relationship; step S130, obtain the experimental data point set corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship; step S140, obtain the light intensity mean of the air chamber stage based on the experimental data point set corresponding to the air chamber position stage obtained in step S120 , based on the experimental data point set corresponding to the hemoglobin cavity position stage obtained in step S130, the light intensity mean value of the hemoglobin cavity position stage is obtained. Step S150: Based on the formula of Lambert-Beer's law and the mean light intensity , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When the concentration of the hemoglobin solution is known, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0071] It should be understood that in the embodiments of the present application, the processor may be a central processing unit (CPU), and the processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. Among them, the general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0072] It can be understood by those skilled in the art that all or part of the processes in the method for implementing the above embodiment can be completed by instructing the relevant hardware through a computer program. The computer program includes program instructions, and the computer program can be stored in a storage medium, which is a computer-readable storage medium. The program instructions are executed by at least one processor in the computer system to implement the process steps of the embodiment of the above method.

[0073] Therefore, the present invention also provides a storage medium. The storage medium may be a computer-readable storage medium. The storage medium stores a computer program, wherein the computer program includes program instructions. When the program instructions are executed by a processor, the processor executes the following steps: Step S110, perform a transmittance test on a counting plate with a hemoglobin sample placed thereon, and obtain a test data point set. , establish a curve graph corresponding to the displacement-light intensity relationship based on the experimental data point set; step S120, obtain the experimental data point set corresponding to the stage when the counting plate moves to the air chamber position in the curve graph corresponding to the displacement-light intensity relationship; step S130, obtain the experimental data point set corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship; step S140, obtain the light intensity mean of the air chamber stage based on the experimental data point set corresponding to the air chamber position stage obtained in step S120 , based on the experimental data point set corresponding to the hemoglobin cavity position stage obtained in step S130, the light intensity mean value of the hemoglobin cavity position stage is obtained. Step S150: Based on the formula of Lambert-Beer's law and the mean light intensity , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When the concentration of the hemoglobin solution is known, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

[0074] The storage medium may be a USB flash drive, a mobile hard disk, a read-only memory (ROM), a magnetic disk, or an optical disk, etc., which are computer-readable storage media that can store program codes.

[0075] In summary, the hemoglobin concentration measurement system and method, device and storage medium of the present invention obtains the experimental data point set corresponding to the air cavity position stage and the experimental data point set corresponding to the hemoglobin cavity position stage in the curve diagram corresponding to the displacement-light intensity relationship, thereby obtaining the light intensity mean value of the air cavity stage. and the average light intensity at the hemoglobin cavity position Finally, based on the Beer-Lambert law formula, the hemoglobin concentration test result is quickly and accurately obtained, thereby avoiding the problem that the counting plate or the measuring component cannot be moved to the desired position, resulting in deviation in the final hemoglobin measurement result.

[0076] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, computer software, or a combination of the two. In order to clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in terms of function in the above description. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of the present invention.

[0077] In the several embodiments provided by the present invention, it should be understood that the disclosed systems and methods can be implemented in other ways. For example, the system embodiments described above are only schematic. For example, the division of each unit is only a logical function division, and there may be other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed.

[0078] The steps in the method of the embodiment of the present invention can be adjusted in order, combined and deleted according to actual needs. The units in the device of the embodiment of the present invention can be combined, divided and deleted according to actual needs. In addition, the functional units in the various embodiments of the present invention can be integrated into a processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a storage medium. Based on this understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product, which is stored in a storage medium and includes a number of instructions for a device (which can be a personal computer, a terminal, or a network device, etc.) to perform all or part of the steps of the method described in the various embodiments of the present invention.

[0079] The above-described embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A method for measuring hemoglobin concentration, characterized in that: The steps include: Step S110: Perform a transmittance test on the counting plate with the hemoglobin sample placed thereon to obtain a test data point set. , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set; Step S120, obtaining a set of test data points corresponding to the stage when the counting plate moves to the air chamber position in the curve diagram corresponding to the displacement-light intensity relationship; Step S130, obtaining a set of experimental data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship; Step S140: Obtain the light intensity mean value of the air cavity stage based on the experimental data point set corresponding to the air cavity position stage obtained in step S120. , based on the experimental data point set corresponding to the hemoglobin cavity position stage obtained in step S130, the light intensity mean value of the hemoglobin cavity position stage is obtained. ; Step S150: Based on the Beer-Lambert law formula and the light intensity mean , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When is the hemoglobin concentration value of a known hemoglobin solution, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

2. The hemoglobin concentration measurement method according to claim 1, characterized in that: The method of step S110 is specifically performed as follows: The external driving device drives the counting board to move different displacements along the moving slide rail, and the light signal emitted by the transmitter is received by the photoelectric conversion module to obtain the light intensity received by the photoelectric conversion module corresponding to the different displacements of the counting board; The displacement-light intensity curves corresponding to the movement of the counting plate to multiple position stages in the curve graph corresponding to the displacement-light intensity relationship are obtained, and the displacement-light intensity curves corresponding to the multiple position stages are combined into a curve graph corresponding to the displacement-light intensity relationship.

3. The hemoglobin concentration measurement method according to claim 1, characterized in that: The method of step S120 comprises the following steps: Obtain light intensity values ​​of test data points corresponding to a plurality of peaks and a plurality of troughs in a curve graph corresponding to a displacement-light intensity relationship; Obtain a test data point corresponding to a peak corresponding to the maximum light intensity in a curve graph corresponding to the displacement-light intensity relationship, and record it as the first test data point; In the curve graph corresponding to the displacement-light intensity relationship, obtain a second test data point corresponding to the trough close to the first test data point, and record it as the second test data point; The first k test data points and the last k test data points close to the second test data point in the curve graph corresponding to the displacement-light intensity relationship are obtained, and the test data point set corresponding to the stage when the counting plate moves to the air chamber position is obtained. The test data point set corresponding to the stage when the counting plate moves to the air chamber position includes the first test data point, the first k test data points and the last k test data points close to the second test data point in the curve graph corresponding to the displacement-light intensity relationship.

4. The hemoglobin concentration measurement method according to claim 3, characterized in that: The maximum value of k can be ⌊ ⌋, where d refers to the displacement of the counting plate when collecting adjacent test data points, and L refers to the preset length of the air chamber stage on the counting plate.

5. The hemoglobin concentration measurement method according to claim 1, characterized in that: The method of step S130 comprises the following steps: Obtain light intensity values ​​of test data points corresponding to a plurality of peaks and a plurality of troughs in a curve graph corresponding to a displacement-light intensity relationship; Obtain a test data point corresponding to a peak corresponding to the maximum light intensity in a curve graph corresponding to the displacement-light intensity relationship, and record it as the first test data point; In the curve graph corresponding to the displacement-light intensity relationship, obtain a point whose x-axis coordinate value is greater than the x-axis coordinate value x of the first test data point. t The corresponding y-axis coordinate values ​​of the multiple test data points are recorded as y1, y2…y n-1 ,y n ; Based on y n -y n-1 ≤△T to obtain the experimental data point set corresponding to the stage when the counting plate moves to the hemoglobin chamber position, where △T is the preset threshold.

6. The hemoglobin concentration measurement method according to claim 1, characterized in that: In step S140 .

7. The hemoglobin concentration measurement method according to claim 1, characterized in that: In step S140 .

8. A hemoglobin concentration measurement system, characterized in that: include: The acquisition module is used to perform a transmittance test on the counting plate with the hemoglobin sample placed on it and obtain a set of test data points. , a curve graph corresponding to the displacement-light intensity relationship is established based on the experimental data point set; The first acquisition module is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the air chamber position in a curve diagram corresponding to the displacement-light intensity relationship; The second acquisition module is used to obtain a set of test data points corresponding to the stage when the counting plate moves to the hemoglobin chamber position in the curve graph corresponding to the displacement-light intensity relationship; The first calculation module is used to obtain the experimental data point set corresponding to the air cavity position stage to obtain the light intensity mean of the air cavity stage The light intensity mean of the hemoglobin cavity position stage is obtained by using the experimental data point set corresponding to the hemoglobin cavity position stage ; The second calculation module is used based on the formula of Lambert-Beer law and the mean light intensity , Get the hemoglobin concentration of the hemoglobin solution to be tested ,exist When is the hemoglobin concentration value of a known hemoglobin solution, I0 refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the known hemoglobin solution cavity of the counting plate, and I refers to the light intensity of the light received by the photoelectric conversion module after the initial light emitted by the emitter passes through the air cavity of the counting plate.

9. A smart device, characterized in that: The device includes a memory and a processor, wherein a computer program is stored in the memory, and when the processor executes the computer program, the hemoglobin concentration measurement method according to any one of claims 1 to 7 is implemented.

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