Containers for low temperature storage of agricultural and horticultural crops

Abstract

The object of the present invention is to provide a new type of container, a new type of packaging body and a new type of storage method, which can effectively inhibit the significant reduction in the commercial value of agricultural and horticultural crops caused by mold, and in addition to not causing odor transfer caused by antimicrobial active ingredients, it will not cause coloring or quality deterioration, and is suitable for circulation or storage. As a solution, a container for low-temperature storage of agricultural and horticultural crops is provided, characterized in that it has a sustained-release antimicrobial film and satisfies the following conditions 1 to 3, wherein the sustained-release antimicrobial film is formed by laminating the following layers in sequence: a substrate film layer; a volatile antimicrobial active ingredient layer containing one or more volatile antimicrobial active compounds selected from the group of volatile antimicrobial active compounds with a boiling point in the range of 100 to 240°C; and a volatile antimicrobial active ingredient penetration layer composed of a vapor-deposited polymer film. Condition 1: The volume of the storage container is 25 cm 3 Over 250,000cm 3 Condition 2: The area of ​​the sustained-release antibacterial film is 1 cm 2 Over 5,000cm 2 Condition 3: Area of ​​sustained-release antibacterial film (cm 2 ) relative to the volume of the storage container (cm 3 ) is greater than 0.02.

Description

Technical Field

[0001] The present invention relates to a container for low-temperature storage of agricultural and horticultural crops having a slow-release antibacterial film, a package containing agricultural and horticultural crops, and a method for low-temperature storage of agricultural and horticultural crops. Background Art

[0002] In the past, as a long-term storage method for harvested common crops, vegetables, fruits, flowers, mushrooms and other agricultural and horticultural crops, there are known methods of appropriately suppressing the respiration of agricultural and horticultural crops by sealing in deoxidizers, etc., or storage methods at ice temperatures below 0°C (for example, Patent Document 1, etc.), but these methods have the problem of being unable to suppress the production of mold. In Japan, the use of pesticides such as fungicides is not allowed when agricultural and horticultural crops are circulated, and their use is also subject to greater restrictions in overseas countries such as Europe. Therefore, as a method for preventing mold in agricultural and horticultural crops, there is a known method of sterilizing the harvested agricultural and horticultural crops in advance with a hypochlorous acid disinfectant, but hypochlorous acid disinfectants require great attention when used, and there are problems such as being unable to obtain long-term effects.

[0003] On the other hand, as a method for imparting antibacterial properties to resin films used for packaging agricultural and horticultural crops, there are known methods of coating the film surface with a vaporizing antibacterial agent (e.g., Patent Document 2, etc.), or mixing an inorganic antibacterial agent or an organic antibacterial agent in a resin (e.g., Patent Documents 3, 4, etc.). The former coating method is difficult to control the amount of antibacterial agent vaporized from the antibacterial film. If the vaporization concentration of the antibacterial agent increases, not only will the odor be transferred to the agricultural and horticultural crops being packaged, but there is also a risk of quality deterioration such as discoloration. In addition, the latter mixing method also has the following problems: since the antibacterial activity is only produced in the part that contacts the antibacterial agent exposed to the surface of the antibacterial film, it is difficult to show antibacterial activity to the entire agricultural and horticultural crops being packaged, or since the antibacterial agent present in the resin cannot exert antibacterial activity, a large amount of antibacterial agent is required to obtain the desired antibacterial activity.

[0004] Patent Literature

[0005] Patent Document 1: Japanese Patent Application Laid-Open No. 06-046749

[0006] Patent Document 2: Japanese Patent Application Publication No. 2006-199852

[0007] Patent Document 3: Japanese Patent Application Publication No. 2016-030406

[0008] Patent Document 4: Japanese Patent Application Publication No. 2015-189138 Summary of the invention

[0009] The object of the present invention is to provide a new container, a new packaging body and a new storage method, which can effectively inhibit the significant reduction in the commercial value of agricultural and horticultural crops caused by mold, and in addition to not causing odor transfer caused by antimicrobial active ingredients, will not cause quality deterioration such as discoloration, and are suitable for circulation or storage.

[0010] The inventors of the present invention have repeatedly conducted in-depth studies to solve the above-mentioned problems. As a result, they have found that by setting the volume of the storage container and the area of ​​the slow-release antibacterial film, as well as the ratio between the two to a specific range, the significant reduction in the commercial value of agricultural and horticultural crops caused by mold can be effectively suppressed without reducing the quality of agricultural and horticultural crops, thereby solving the above-mentioned problems.

[0011] Specifically, the present invention is gist of the following matters.

[0012] 1. A container for low-temperature storage of agricultural and horticultural crops, characterized in that it has a slow-release antimicrobial film and satisfies the following conditions 1 to 3, wherein the slow-release antimicrobial film is formed by laminating the following layers in order: a substrate film layer; a volatile antimicrobial active ingredient layer containing one or more volatile antimicrobial active compounds selected from the group of volatile antimicrobial active compounds having a boiling point in the range of 100 to 240° C.; and a volatile antimicrobial active ingredient permeation layer composed of a vapor-deposited polymer film,

[0013] Condition 1: The volume of the storage container is 25 cm 3 Over 250,000cm 3 the following;

[0014] Condition 2: The area of ​​the sustained-release antibacterial film is 1 cm 2 Over 5,000cm 2 the following;

[0015] Condition 3: Area of ​​sustained-release antibacterial film (cm 2 ) relative to the volume of the storage container (cm 3 ) is greater than 0.02.

[0016] 2. An agricultural and horticultural crop storage package, characterized in that agricultural and horticultural crops are stored in the low-temperature storage container described in 1.

[0017] 3. A method for preserving agricultural and horticultural crops, characterized in that the agricultural and horticultural crops are stored in the storage container described in 1. at a temperature of -3°C to 15°C.

[0018] The sustained-release antibacterial film of the container of the present invention can stably release the specific volatile antibacterial active compounds contained in the volatile antibacterial active component layer because its volatile antibacterial active component permeation layer is composed of a vapor-deposited polymer film, and by setting the volume of the storage container (condition 1) and the area of ​​the sustained-release antibacterial film (condition 2) to a specific range, and further setting the ratio of the area to the volume to a specific value or more (condition 3), the amount of volatile antibacterial active components in the storage container can be made to reach an appropriate range. The present invention can effectively inhibit the significant reduction in the commercial value of agricultural and horticultural crops caused by mold without reducing the quality of agricultural and horticultural crops through these synergistic effects.

[0019] The volatile antimicrobial active compound having a specific boiling point range used in the present invention is also a plant aroma component used as a food additive, and therefore the present invention is also useful in safely preserving agricultural and horticultural crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 These are photographs for explaining the configurations 1 to 4 of the containers used for the storage test in the test 1 of the low-temperature storage of agricultural and horticultural crops in the examples. DETAILED DESCRIPTION

[0021] The container for low-temperature storage of agricultural and horticultural crops and the method for storing agricultural and horticultural crops using the container according to the present invention are described in detail below. In addition, the "low temperature" in the present invention refers to a temperature range of -3°C or higher and 15°C or lower.

[0022] <About agricultural and horticultural crops>

[0023] The container for low-temperature storage of agricultural and horticultural crops in the present invention is a container for storing agricultural and horticultural crops. The agricultural and horticultural crops in the present invention refer to all cultivated crops such as common crops, vegetables, fruits, flowers, mushrooms, etc. There are no special restrictions on agricultural and horticultural crops, for example, common crops such as rice, wheat, corn, potatoes, beans, root vegetables such as radish, carrots, burdock, stem vegetables such as onions, asparagus, edible angelica, leafy vegetables such as cabbage, lettuce, spinach, Chinese cabbage, fruit vegetables such as tomatoes, eggplants, pumpkins, cauliflowers, broccoli, edible chrysanthemums, etc.; fruits such as citrus, apples, pears, grapes, blueberries, strawberries, cherries, peaches, etc.; flowers such as cut flowers; mushrooms such as Shimeji Mushroom, Matsutake, and Maitake.

[0024] Among them, from the perspective of being suitable for low-temperature storage and obtaining an effective effect of inhibiting mold production, fruits are more suitable as agricultural and horticultural crops in the present invention, among which grapes, cherries, peaches, and strawberries are more suitable. For grapes, cherries, peaches, and strawberries, a temperature range of 0°C to 5°C is a more suitable storage temperature.

[0025] <About volatile antimicrobial active ingredients>

[0026] The present invention uses at least one volatile antimicrobial active component selected from the group of volatile antimicrobial active compounds having a boiling point in the range of 100 to 240°C.

[0027] As the compound contained in the volatile antimicrobial active compound group of the present invention, it is preferred to form a compound with a carbon number of 5 or more and 10 or less. As specific examples of suitable compounds, for example, hinokitiol (boiling point: 140°C), citronellal (boiling point: 204-206°C), geraniol (trans-2-hexenal, boiling point: 146°C), citronellol (boiling point: 225°C), geraniol (boiling point: 229°C), nerol (boiling point: 224-225°C / 745mmHg), etc. Among them, volatile antimicrobial active compounds with a boiling point in the range of 100-200°C are preferred, and hinokitiol and geraniol are particularly preferred.

[0028] For the formation of the volatile antimicrobial active ingredient layer of the present invention, a method corresponding to the form or properties of the volatile antimicrobial active ingredient used can be appropriately selected. For example, if it is a volatile antimicrobial active ingredient that is liquid at room temperature, it can be directly applied to one side of the substrate film layer without dilution to form a volatile antimicrobial active ingredient layer. Even if it is a volatile antimicrobial active ingredient that is solid at room temperature or a volatile antimicrobial active ingredient that is liquid at room temperature, a solution can be prepared by a prescribed solvent, such as ethanol, and applied to one side of the substrate film layer, and then the solvent is evaporated to form a volatile antimicrobial active ingredient layer that is essentially only composed of volatile antimicrobial active ingredients. In addition, a vapor deposition treatment method can also be selected, and the vapor deposition treatment method is to heat the volatile antimicrobial active ingredient to vaporize or sublimate it, and adhere to one side of the substrate film layer, thereby forming a volatile antimicrobial active ingredient layer that is only composed of volatile antimicrobial active ingredients.

[0029] The thickness of the volatile antimicrobial active ingredient layer in the present invention may be appropriately determined according to the type of volatile antimicrobial active ingredient selected or the desired antimicrobial activity maintenance period, and is generally in the range of 1 μm to 5 mm.

[0030] <About the penetration of volatile antimicrobial active ingredients into the layer>

[0031] The volatile antimicrobial active ingredient permeable layer in the present invention is composed of a vapor-deposited polymer film, which is a thin film obtained by vapor deposition polymerization.

[0032] Evaporation polymer film is generally by introducing two or more raw material monomers vaporized by heating into the film forming chamber of vacuum state, making it adhere to the surface of the film forming object configured in the film forming chamber, and polymerizing between the raw material monomers on the surface to form. That is, the formation of the film is carried out by tracking the fine concavoconvex on the surface of the volatile antimicrobial active component layer present in the present invention, thereby forming a volatile antimicrobial active component penetration layer, so that excellent adhesion is exerted between the volatile antimicrobial active component layer and the volatile antimicrobial active component penetration layer. In addition, the evaporation polymer film can also be made under the extremely thin film thickness state, and the obtained evaporation polymer film also has the advantage of containing very few impurities. Therefore, the sustained-release antimicrobial film in the present invention, between the volatile antimicrobial active component layer and the volatile antimicrobial active component penetration layer, can advantageously suppress the generation of interlayer peeling caused by hydrolysis or thermal shock, so as to stably release and volatilize the volatile antimicrobial active component amount that meets the target.

[0033] As the synthetic resin constituting the volatile antimicrobial active ingredient penetration layer in the present invention, a material corresponding to the volatile antimicrobial active ingredient can be appropriately selected from the synthetic resins that are known in the past and can form a film according to the vapor deposition polymerization method. Specifically, as the synthetic resin film constituting the volatile antimicrobial active ingredient penetration layer, various synthetic resin films that can be made according to the known vapor deposition polymerization method, such as polyurea resin film, polyamide resin film, polyimide resin film, polyamide-imide resin film, polyester resin film, polyazomethine resin film or polyurethane resin film, can be exemplified. Among them, the polyurea resin film has a cross-linked structure that allows the volatile antimicrobial active ingredient to penetrate effectively, and therefore can be advantageously adopted.

[0034] In addition, the thickness of the volatile antimicrobial active ingredient penetration layer can be appropriately determined by comprehensively considering the amount of volatile antimicrobial active ingredients released per unit time to the outside of the film, or the type or amount of volatile antimicrobial active ingredients contained in the volatile antimicrobial active ingredient layer, etc. Specifically, the thickness of the volatile antimicrobial active ingredient penetration layer is preferably in the range of 0.01 μm to 50 μm, and more preferably in the range of 0.1 μm to 10 μm.

[0035] <About sustained-release antimicrobial film>

[0036] The sustained-release antimicrobial film of the present invention is preferably in the following form: a volatile antimicrobial active ingredient layer is formed on one surface of a substrate film layer, and a volatile antimicrobial active ingredient permeation layer composed of a vapor-deposited polymer film is further formed on the surface of the volatile antimicrobial active ingredient layer. The substrate film layer, the volatile antimicrobial active ingredient layer, and the volatile antimicrobial active ingredient permeation layer are preferably substantially the same in size.

[0037] The sustained-release antimicrobial film of the present invention is preferably disposed on the upper part of the container for low-temperature storage of agricultural and horticultural crops, and more preferably disposed on the top surface of the container by covering the surface of the agricultural and horticultural crops inside the container, or by pasting, etc. In this case, it is preferably disposed in a manner such that the volatile antimicrobial active ingredients in the sustained-release antimicrobial film penetrate the layer and face the agricultural and horticultural crops inside the container.

[0038] The container for low-temperature storage of agricultural and horticultural crops of the present invention is characterized in that, as "Condition 2", a 1 cm 2 Over 5,000cm 2 The area of ​​the sustained-release antibacterial film is preferably 5 cm 2 Over 1,000cm 2 Less than, more preferably 10cm 2 Over 500cm 2 the following.

[0039] The slow-release antimicrobial film of the container for low-temperature storage of agricultural and horticultural crops of the present invention may be one sheet or two or more sheets. When multiple sheets are used, the area of ​​the slow-release antimicrobial film in the present invention refers to the total area of ​​all the slow-release antimicrobial films used.

[0040] <Regarding containers for low-temperature storage of agricultural and horticultural crops>

[0041] The container for low temperature storage of agricultural and horticultural crops of the present invention is not particularly limited in terms of materials such as resin or paper, or in terms of shapes such as bag and box, among which the box shape is more suitable. However, in order to maintain the amount of volatile antimicrobial active ingredients volatilized from the sustained-release antimicrobial film of the present invention, a container that can be sealed to a certain extent is preferred.

[0042] The container for low-temperature storage of agricultural and horticultural crops of the present invention can be a container composed of the slow-release antibacterial film of the present invention, but in a storage container that also includes a bag-shaped article composed of other materials different from the slow-release antibacterial film of the present invention, it is simpler and more cost-effective to store agricultural and horticultural crops and arrange the slow-release antibacterial film of the present invention on the upper part of the stored agricultural and horticultural crops.

[0043] The container for low-temperature storage of agricultural and horticultural crops of the present invention is characterized in that, as "Condition 1", its volume is 25 cm 3 Over 250,000cm 3 The storage container preferably has a capacity of 500 cm 3 Over 100,000cm 3 Below, more preferably 1000cm 3 Over 50,000cm 3 the following.

[0044] <About the amount of volatile antimicrobial active ingredients>

[0045] Since the container for low-temperature storage of agricultural and horticultural crops of the present invention can make the amount of volatile antimicrobial active ingredients in the storage container reach an appropriate range, the quality of agricultural and horticultural crops will not be reduced due to odor transfer or discoloration caused by volatile antimicrobial active ingredients, and the significant reduction in the commercial value of agricultural and horticultural crops caused by mold can be effectively inhibited.

[0046] In general, if a sustained-release antimicrobial film is placed in a sealed container, the amount of antimicrobial components in the container will increase over time. If the amount of antimicrobial components in the container is small, sufficient antimicrobial activity cannot be obtained, which will cause the agricultural and horticultural crops to rot. On the other hand, if the amount of antimicrobial components in the container becomes too much, the commodity value of the agricultural and horticultural crops will be reduced due to the odor transfer or discoloration of the volatile antimicrobial active components. That is, in order to effectively inhibit the significant reduction in the commodity value of agricultural and horticultural crops caused by mold, and to inhibit the odor transfer caused by the volatile antimicrobial active components, in addition to inhibiting quality deterioration such as discoloration, it is necessary to control the amount of antimicrobial components in the storage container within an appropriate range.

[0047] The present invention can control the amount of antimicrobial components in the storage container within an appropriate range, and for the first time determines the range of the area of ​​the sustained-release antimicrobial film and the volume of the storage container required for this purpose, as well as the relationship between the sustained-release antimicrobial film and the storage container.

[0048] The volatile antimicrobial active ingredient in the present invention is one or more substances selected from the group of volatile antimicrobial active compounds having a boiling point in the range of 100 to 240° C. This is one of the conditions for making the amount of the antimicrobial ingredient in the storage container within an appropriate range.

[0049] As described above, by making the amount of volatile antimicrobial active ingredients in the container for low-temperature storage of agricultural and horticultural crops of the present invention reach an appropriate range, the significant reduction in the commercial value of agricultural and horticultural crops caused by mold can be effectively suppressed. In addition to not causing odor transfer caused by antimicrobial active ingredients, quality deterioration such as discoloration will not occur, and a container for low-temperature storage of agricultural and horticultural crops suitable for circulation or storage can be made.

[0050] In order to make the amount of the volatile antimicrobial active ingredient in the container for low-temperature storage of agricultural and horticultural crops of the present invention fall within an appropriate range, the following conditions 1 to 3 need to be satisfied.

[0051] [Condition 1]: The volume of the storage container is 25 cm 3 Over 250,000cm 3 the following;

[0052] [Condition 2]: The area of ​​the sustained-release antibacterial film is 1 cm 2 Over 5,000cm 2 the following;

[0053] [Condition 3]: Area of ​​sustained-release antibacterial film (cm 2 ) relative to the volume of the storage container (cm 3 ) is greater than 0.02.

[0054] The above-mentioned "Condition 3" is explained.

[0055] "Condition 3" is to set the area (cm 2 ) relative to the volume of the storage container (cm 3 ) ratio, that is, the area of ​​the sustained-release antibacterial film (cm 2 ) divided by the volume of the storage container (cm 3 ) is set to a value of 0.02 or more.

[0056] In order to make the amount of volatile antimicrobial active ingredients in the container for low-temperature storage of agricultural and horticultural crops of the present invention reach an appropriate range, it is particularly important to satisfy this "condition 3". When the ratio of this "condition 3" is less than 0.02, the amount of volatile antimicrobial active ingredients in the container for low-temperature storage of agricultural and horticultural crops is insufficient, and the anti-mold effect on the agricultural and horticultural crops in the storage container cannot be obtained. By setting the ratio of "condition 3" to 0.02 or more, the following container for low-temperature storage of agricultural and horticultural crops can be made, which can effectively inhibit the significant reduction in the commercial value of agricultural and horticultural crops caused by mold, and in addition to not causing odor transfer caused by antimicrobial active ingredients, it will not cause quality deterioration such as discoloration, and is suitable for circulation or storage.

[0057] Example

[0058] The present invention is described below with reference to the following examples, but the technical scope of the present invention is not limited thereto.

[0059] <Preparation of sustained-release antibacterial film>

[0060] Using a roll-to-roll vapor deposition polymer film forming apparatus, hinokitiol, a volatile antimicrobial active compound, was deposited on a polyethylene terephthalate (PET) film (thickness: 12 μm) at an effective amount of 1.3 mg / cm 2 Then, a volatile antimicrobial active ingredient layer was formed by using the same vapor-deposited polymer film forming apparatus to form a vapor-deposited polymer film made of polyurea resin. The vapor-deposited polymer film made of polyurea resin was produced with a thickness of 4 μm.

[0061] <Experiment 1 on cryopreservation of agricultural and horticultural crops>

[0062] As a storage container, a sealed container with a drain box 1 (capacity: 1,100 cm 3 , bottom size: outer dimension 150mm×134mm, height: outer dimension 77mm). Figure 1 As shown, a paper towel soaked in distilled water (20 mL) was laid on the bottom of the storage container to maintain humidity ( Figure 1 1), place the packaging sponge in the drain box ( Figure 1 2), the prepared sustained-release antibacterial film 1 (area: 78 cm) was pasted on the inner side of the lid of the storage container with a double-sided tape. 2 )( Figure 1 The above storage container is about 0.071 in "Condition 3" of the present invention.

[0063] As the agricultural and horticultural crops to be preserved, Shine Muscat grape berries were used. Figure 1 As shown, put 12 capsules (such as Figure 1 4), spray with sterile water, and air dry before use. Install it on Figure 1 1 in the middle and cover with lid ( Figure 1 3) in the experiment, and use this as Example 1 to carry out the preservation test.

[0064] A storage container different only in that the sustained-release antimicrobial film was not used was used as Comparative Example 1, and the same storage test was performed.

[0065] The rotten fruits in each test plot were observed every week from the start of storage, and the number was counted. In addition, if rotten fruits were observed, they were removed.

[0066] The storage test was carried out three times, and the average value of the cumulative spoilage rate (%) was calculated. Table 1 summarizes the test results at a storage temperature of 5°C.

[0067] [Table 1]

[0068]

[0069] As shown in Table 1, the container for low-temperature storage of agricultural and horticultural crops of the present invention (Example 1) had no rotten fruit even after 70 days (2.5 months) from the start of storage, and the average cumulative rotten fruit rate after 91 days (more than 3 months) from the start of storage was 11.1%. In addition, no quality deterioration such as odor transfer or discoloration caused by volatile antimicrobial active ingredients was confirmed in the fruit grains after 91 days from the start of storage. In contrast, when a storage container different from the container of the present invention was used (Comparative Example 1), the average cumulative rotten fruit rate was 8.3% at the time point of 21 days (3 weeks) from the start of storage, and reached 69.4% after 91 days (more than 3 months).

[0070] That is, it was found that the use of the container for low-temperature storage of agricultural and horticultural crops of the present invention can effectively suppress the corruption of agricultural and horticultural crops such as Champignon grapes, which are expensive and have high commercial value, due to mold.

[0071] On the other hand, although detailed test data are omitted, when the low-temperature storage container for agricultural and horticultural crops of the present invention is used and stored at 20°C, which is outside the definition of low temperature of the present invention, brown spots appear on the surface of the fruit grains until 21 days (3 weeks), and then all the fruit grains are confirmed to be corrupted until 49 days (7 weeks). This result indirectly indicates that even if the low-temperature storage container for agricultural and horticultural crops of the present invention is used, under conditions other than low-temperature storage, quality deterioration such as discoloration caused by the antimicrobial active ingredient will occur, and corruption will be promoted later.

[0072] <Experiment 2 on low temperature storage of agricultural and horticultural crops>

[0073] In each of the sealed containers 1 (capacity: 1,100 cm) with the same drain box as in the above-mentioned "Experiment 1 on low temperature storage of agricultural and horticultural crops" 3 , bottom size: outer size 150mm×134mm, height: outer size 77mm) using 12 fruit pieces and 1 sustained-release antibacterial film (area: 78cm 2 ), in the same container 2 (capacity: 5,000cm 3 , bottom size: outer size 213mm×263mm, height: outer size 130mm) using 25 fruit pieces and 2 sustained-release antibacterial films (area: 312cm 2 ) and installed in the same manner as in the above-mentioned "Experiment 1 on low temperature storage of agricultural and horticultural crops".

[0074] As the agricultural and horticultural crops to be stored, the berries of the grapes of the grapes were spray-inoculated with a suspension of conidia of Botrytis cinerea (106 / mL) and then air-dried, and the storage test was carried out at a storage temperature of 5° C. In addition, the container 1 (Example 2, Comparative Example 2) was filled with distilled water (20 mL), and the container 2 (Example 3, Comparative Example 3) was filled with distilled water (100 mL) to maintain humidity.

[0075] The rotten fruits in each test plot were observed every week from the start of storage, and the number was counted. In addition, if rotten fruits were observed, they were removed.

[0076] The preservation test was carried out 5 times in Example 2 and Comparative Example 2, and 4 times in Example 3 and Comparative Example 3, and the average value of the cumulative spoilage rate (%) was calculated.

[0077] Table 2 summarizes the test results.

[0078] [Table 2]

[0079]

[0080] This "Test 2 on Low Temperature Storage of Agricultural and Horticultural Crops" is a storage test that simulates a very harsh environment of mandatory inoculation with Botrytis cinerea.

[0081] As shown in Table 2, in Comparative Example 2 using a storage container different from that of the present invention, all the test fruit grains were corrupted on the 28th day from the start of storage. Similarly, in Comparative Example 3 using a storage container different from that of the present invention, 98% of the test fruit grains were corrupted.

[0082] On the other hand, even in the storage test under such extremely severe conditions, in the low-temperature storage container for agricultural and horticultural crops of the present invention (Examples 2 and 3), the spoilage of agricultural and horticultural crops caused by mold was only about one-fifth or less of the test fruit grains, and in Example 3 in particular, it was only 6% of the test fruit grains, which clearly showed that the spoilage inhibition effect was extremely excellent. In addition, it was also clear that the excellent spoilage inhibition effect was exerted regardless of the volume difference of the storage container under "Condition 1".

[0083] In addition, in Examples 2 and 3, no quality deterioration such as odor transfer or color change due to the antimicrobial active ingredient was observed in the fruit grains after 28 days from the start of storage.

[0084] <Experiment 3 on cryopreservation of agricultural and horticultural crops>

[0085] In a sealed container 1 (capacity: 1,100 cm 3, bottom size: outer size 150mm×134mm, height: outer size 77mm), 12 fruit pieces, 1 sustained-release antibacterial film (area: 78cm 2 )、Sustained-release antibacterial film 3 (area: 39cm 2 )、Sustained-release antibacterial film 4 (area: 19cm 2 ) were installed in the same manner as in the above-mentioned "Test 1 on low temperature storage of agricultural and horticultural crops", and distilled water (20 mL) was used to maintain humidity.

[0086] As the agricultural and horticultural crops to be preserved, the preservation test was carried out at a temperature of 5°C using grape berries that were spray-inoculated with a conidia suspension of Botrytis cinerea (106 conidia / mL) and then air-dried.

[0087] The rotten fruits in each test plot were observed every week from the start of storage, and the number was counted. In addition, if rotten fruits were observed, they were removed.

[0088] The storage test was carried out 4 times, and the average value of the cumulative spoilage rate (%) was calculated.

[0089] Table 3 summarizes the test results.

[0090] [Table 3]

[0091]

[0092] This "Test 3 on Low Temperature Storage of Agricultural and Horticultural Crops" is a storage test that simulates a very harsh environment of mandatory inoculation with Botrytis cinerea.

[0093] As shown in Table 3, in Comparative Example 5 using a storage container different from that of the present invention, 97.9% of the test fruit particles were spoiled on the 28th day from the start of storage.

[0094] On the other hand, even in the storage test under such extremely harsh conditions, in the low-temperature storage container for agricultural and horticultural crops of the present invention (Examples 4 and 5), after 70 days (2.5 months) from the start of storage, the corruption of agricultural and horticultural crops due to mold was only less than about 30% of the test fruit particles, which clearly shows that the corruption inhibition effect is extremely excellent.

[0095] On the other hand, in Comparative Example 4, due to the "Condition 3" in the present invention, that is, the area (cm 2 ) relative to the volume of the storage container (cm 3 ) is about 0..017, which is different from the storage container of the present invention. Therefore, it was confirmed that under the storage of Comparative Example 4, the corruption of agricultural and horticultural crops caused by mold from the beginning of storage to the 28th day or later progressed to about 2.5 to 4 times that of the example.

[0096] <Experiment 4 on low temperature storage of agricultural and horticultural crops>

[0097] Into the sealed container 3 with drain box (capacity: 0.0030m 3 , bottom size: outer size 239mm×176mm, height: outer size 91mm) were placed in 41 fruit pieces, and 5 slow-release antibacterial films (area: 233cm 2 )、Sustained-release antibacterial film 1 (area: 78cm 2 ), in the same manner as in the above-mentioned "Test 1 on low temperature storage of agricultural and horticultural crops", after spraying with sterilized water (not inoculated), the air-dried fruit particles were subjected to a storage test at the storage temperature shown in Table 4. In addition, container 3 was kept humid using distilled water (60 mL).

[0098] The rotten fruits in each test plot were observed every week from the start of storage, and the number was counted. In addition, if rotten fruits were observed, they were removed.

[0099] The storage test was carried out three times, and the average value of the cumulative spoilage rate (%) was calculated.

[0100] The test results are summarized in Table 4. In addition, since the results of Comparative Examples 8 and 9 from the start of storage to the 21st day showed significant differences from those of Examples 6 and 7, it was determined that further process observation was not necessary and the experiment was terminated. "-" in Table 4 means that the test observation was not carried out.

[0101] [Table 4]

[0102]

[0103] As shown in Table 4, it is clear that the containers for low-temperature storage of agricultural and horticultural crops (Examples 6 and 7) of the present invention are extremely excellent in the effect of inhibiting the spoilage of agricultural and horticultural crops caused by molds in the storage without inoculation of gray mold in a storage state simulating a normal storage state, compared with the storage containers different from the containers of the present invention (Comparative Examples 6 and 7). In addition, the fruit grains of Examples 6 and 7 were not found to have quality deterioration such as odor transfer or color change caused by the antimicrobial active ingredients after 28 days from the start of storage.

[0104] On the other hand, it was also clarified that when the storage was carried out at 23°C, which is outside the definition of low temperature of the present invention (Comparative Examples 8 and 9), even if a sustained-release antibacterial film (Comparative Example 8) was used, the effect of inhibiting spoilage caused by mold could not be obtained. Furthermore, in Comparative Example 8, discoloration was observed from the beginning of storage to about the 10th day. This result indirectly indicates that even if the container for low-temperature storage of agricultural and horticultural crops of the present invention is used, quality deterioration such as discoloration caused by the antibacterial active ingredient will occur under conditions other than low-temperature storage, and then spoilage will be promoted.

[0105] Industrial Applicability

[0106] The sustained-release antibacterial film of the container of the present invention can stably release the specific volatile antibacterial active compounds contained in the volatile antibacterial active component layer because its volatile antibacterial active component permeation layer is composed of a vapor-deposited polymer film, and by setting the volume of the storage container (condition 1) and the area of ​​the sustained-release antibacterial film (condition 2) to a specific range, and further setting the ratio of the area to the volume to a specific value or more (condition 3), the amount of volatile antibacterial active components in the storage container can be made to reach an appropriate range. Through these synergistic effects, the significant reduction in the commercial value of agricultural and horticultural crops caused by mold can be effectively suppressed without reducing the quality of agricultural and horticultural crops, so it is very useful.

Claims

1. A box-shaped container for low-temperature storage of agricultural and horticultural crops, characterized in that: A box-shaped container for storage having a sustained-release antibacterial film and a material different from the sustained-release antibacterial film, wherein the sustained-release antibacterial film is disposed on the upper surface of the box-shaped container for storage, the low temperature is in the temperature range of -3°C to 15°C, and the following conditions 1 to 3 are satisfied, and the sustained-release antibacterial film is laminated with the following layers in order: Base film layer; A volatile antimicrobial active ingredient layer containing at least one volatile antimicrobial active compound selected from the group consisting of volatile antimicrobial active compounds having a boiling point in the range of 100 to 240° C.; and a volatile antimicrobial active ingredient penetration layer consisting of a vapor-deposited polymer film, Condition 1: The volume of the box-shaped container for storage is 25 cm 3 Over 250,000cm 3 the following; Condition 2: The area of ​​the sustained-release antibacterial film is 1 cm 2 Over 5,000cm 2 the following; Condition 3: Area of ​​sustained-release antibacterial film (cm 2 ) relative to the volume of the storage container (cm 3 ) is greater than or equal to 0.02 and less than or equal to 0.

078.

2. A box-shaped container for low-temperature storage of agricultural and horticultural crops according to claim 1, characterized in that: The sustained-release antimicrobial film is disposed on the top surface of the box-shaped container for storage.

3. A storage package for agricultural and horticultural crops, characterized in that: The low-temperature storage box-shaped container according to claim 1 contains agricultural and horticultural crops.

4. A method for preserving agricultural and horticultural crops, characterized in that: The agricultural and horticultural crops are stored in the box-shaped container for storage according to claim 1 and stored at -3°C or higher and 15°C or lower.

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