A reagent development experimental method, device, terminal and storage medium

Abstract

The present invention discloses a reagent development experimental method, device, terminal and storage medium, wherein the method includes: determining multiple process combination results according to the type of immune reagent to be developed; sorting the multiple process combination results as an experimental sequence, setting the experimental sequence and conducting experiments on each process combination result according to the experimental sequence; outputting the experimental results of multiple immune reagents after the experiment, and determining the target immune reagent that meets the preset conditions from the multiple immune reagents based on the performance test results of each immune reagent. The present invention automatically conducts experiments with the predetermined multiple process combination results as the experimental sequence, can quickly generate multiple immune reagents, and the present invention automatically detects the performance of each immune reagent to determine the target immune reagent that meets the preset conditions, which can not only effectively improve the efficiency of reagent development, but also effectively reduce the human error caused by artificial development of reagents, thereby effectively improving the reliability of reagent development.

Description

Technical Field

[0001] The present invention relates to the technical field of reagent development, and in particular to a reagent development experimental method, device, terminal and storage medium. Background Art

[0002] Immunoassay technology is the most widely used biological detection method. It is highly accurate, sensitive, and specific, and can be used in both basic theoretical and applied research in immunology, as well as in various fields of biological research. Immunoassay technology requires the use of immunoreagents to implement various types of immunoassays. Reagent development prior to use is an important and critical process.

[0003] Existing reagent development experimental methods rely too much on manual labor to complete the reagent development process, resulting in low efficiency of reagent development. Summary of the Invention

[0004] The present invention provides a reagent development experimental method, device, terminal and storage medium to solve the technical problem that the existing reagent development experimental method relies too much on manual completion of the reagent development process, resulting in low efficiency of reagent development.

[0005] One embodiment of the present invention provides a reagent development experimental method, comprising:

[0006] Determine the results of multiple process combinations based on the type of immunoassay to be developed;

[0007] sorting the plurality of process combination results as an experimental sequence, setting the experimental sequence, and conducting experiments on the process combination results according to the experimental sequence;

[0008] After the experiment is completed, the experimental results of multiple immune reagents are output, and according to the performance test results of each immune reagent, a target immune reagent that meets the preset conditions is determined from the multiple immune reagents.

[0009] Furthermore, the method of determining the results of combining multiple processes based on the type of immunoassay to be developed includes:

[0010] According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed;

[0011] The processes in each of the process sets are arranged and combined to obtain multiple process combination results.

[0012] Furthermore, the step of determining a target immune reagent that meets preset conditions from a plurality of immune reagents based on the performance test results of each immune reagent includes:

[0013] Obtain the response values of multiple samples with known concentrations;

[0014] Fitting the response values and parameter values of the plurality of samples with known concentrations into a fitting curve;

[0015] Substituting the reaction value of each of the immune reagents into the fitting curve to obtain the parameter value of the immune reagent;

[0016] When the parameter value of the immune reagent meets the preset condition, it is determined that the immune reagent meets the preset condition.

[0017] Furthermore, when the parameter value of the immune reagent meets the preset condition, determining that the immune reagent meets the preset condition includes:

[0018] Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds a preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset condition, and determine that the immune reagent meets the preset condition.

[0019] Furthermore, setting an experimental sequence and conducting experiments on the results of each process combination according to the experimental sequence includes:

[0020] According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores a reagent for detecting the reaction value of a sample with a known concentration. If so, the reagent is called, and the reaction values of multiple samples with a known concentration are obtained using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

[0021] Furthermore, fitting the reaction values and parameter values of the plurality of samples of known concentrations into a fitting curve comprises:

[0022] Combining the response value and parameter value of each sample of known concentration into a data set;

[0023] The plurality of data sets are fitted into a fitting curve using a preset fitting algorithm.

[0024] Furthermore, the reaction value and parameter value of each sample of known concentration are combined into a data set, including:

[0025] Convert the parameter value and the response value of each sample of known concentration into logarithms to form a data set; or

[0026] After setting the number of decimal places for the parameter value and the reaction value of each sample of known concentration, a data group is formed.

[0027] Furthermore, before substituting the reaction values of the respective immune reagents into the fitting curve to obtain the parameter values of the immune reagents, the method further includes:

[0028] Set sampling parameters according to the detection requirements of parameter value detection;

[0029] Collecting multiple original reaction values of each of the immunoreagents during the detection process according to the sampling parameters;

[0030] Plotting the plurality of original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve;

[0031] The average of several original reaction values in the luminescence curve is taken as the reaction value of each immunoreagent.

[0032] Furthermore, before determining the results of combining multiple processes based on the type of immunoassay to be developed, the following steps are also included:

[0033] The position information of multiple reagents inside the detection instrument is obtained, and the position information of the multiple reagents is matched with the corresponding reagent information.

[0034] One embodiment of the present invention provides a reagent development experimental device, comprising:

[0035] A process combination determination module is used to determine multiple process combination results based on the type of immune reagent to be developed;

[0036] An experiment module, configured to sort the plurality of process combination results into an experiment sequence, set the experiment sequence, and conduct experiments on each of the process combination results according to the experiment sequence;

[0037] The reagent determination module is used to output the experimental results of multiple immune reagents after the experiment is completed, and determine the target immune reagent that meets the preset conditions from the multiple immune reagents based on the performance test results of each immune reagent.

[0038] Furthermore, the process combination determination module is used to:

[0039] According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed;

[0040] The processes in each of the process sets are arranged and combined to obtain multiple process combination results.

[0041] Furthermore, the reagent determination module includes:

[0042] A known concentration sample reaction value detection unit is used to obtain reaction values of multiple known concentration samples;

[0043] A fitting unit, configured to fit the reaction values and parameter values of the plurality of samples of known concentrations into a fitting curve;

[0044] a parameter value acquisition unit, configured to substitute the reaction value of each of the immune reagents into the fitting curve to acquire the parameter value of the immune reagent;

[0045] The immune reagent judgment unit is used to judge whether the immune reagent meets the preset conditions when the parameter value of the immune reagent meets the preset conditions.

[0046] Furthermore, the immune reagent determination unit is specifically used to:

[0047] Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds a preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset condition, and determine that the immune reagent meets the preset condition.

[0048] Furthermore, the known concentration sample reaction value detection unit is used to:

[0049] According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores a reagent for detecting the reaction value of a sample with a known concentration. If so, the reagent is called, and the reaction values of multiple samples with a known concentration are obtained by using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

[0050] Furthermore, the fitting unit is specifically used to:

[0051] Combining the response value and parameter value of each sample of known concentration into a data set;

[0052] The plurality of data sets are fitted into a fitting curve using a preset fitting algorithm.

[0053] Furthermore, the reaction value and parameter value of each sample of known concentration are combined into a data set, including:

[0054] Convert the parameter value and the response value of each sample of known concentration into logarithms to form a data set; or

[0055] After setting the number of decimal places for the parameter value and the reaction value of each sample of known concentration, a data group is formed.

[0056] Furthermore, the parameter value obtaining unit is used to:

[0057] Set sampling parameters according to the detection requirements of parameter value detection;

[0058] Collecting multiple original reaction values of each of the immunoreagents during the detection process according to the sampling parameters;

[0059] Plotting the plurality of original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve;

[0060] The average of several original reaction values in the luminescence curve is taken as the reaction value of each immunoreagent.

[0061] Furthermore, the experimental device also includes an information matching module for:

[0062] The position information of multiple reagents inside the detection instrument is obtained, and the position information of the multiple reagents is matched with the corresponding reagent information.

[0063] One embodiment of the present invention provides a terminal, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor implements the above-mentioned reagent development experimental method when executing the computer program.

[0064] One embodiment of the present invention provides a computer-readable storage medium, which includes a stored computer program; wherein, when the computer program is run, it controls the device where the computer-readable storage medium is located to execute the reagent development experimental method as described above.

[0065] The embodiment of the present invention determines multiple process combination results based on the type of immune reagent to be developed, sorts the multiple process combination results as an experimental sequence, conducts experiments on each process combination result according to the experimental sequence to obtain multiple immune reagents, and then determines a target immune reagent that meets the preset conditions by performing performance tests on each immune reagent. The embodiment of the present invention automatically conducts experiments using the predetermined multiple process combination results as the experimental sequence, and can quickly generate multiple immune reagents corresponding to different process combination results. The embodiment of the present invention automatically detects the performance of each immune reagent to determine the target immune reagent that meets the preset conditions, which can not only effectively improve the efficiency of reagent development, but also effectively reduce the human errors caused by the human development of reagents, thereby effectively improving the reliability of reagent development. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] Figure 1 Schematic diagram of the process of the reagent development experimental method provided by the embodiment of the present invention;

[0067] Figure 2 1 is a flow chart of step S3 of the reagent development experimental method provided in an embodiment of the present invention;

[0068] Figure 3Schematic diagram of the structure of the reagent development experimental device provided in an embodiment of the present invention;

[0069] Figure 4 Schematic diagram of the structure of the reagent determination module provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0070] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0071] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of the technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0072] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0073] The reagent development experimental method provided in the embodiment of the present invention is applicable to a variety of immunoassays. The method can be implemented by software and / or hardware and can be configured in a computer device.

[0074] See also Figure 1 One embodiment of the present invention provides a reagent development experimental method, comprising:

[0075] S1. Determine the results of multiple process combinations based on the type of immunoassay to be developed;

[0076] Immunoreagents are reagents made from specific antigens, antibodies or related biological substances. According to different methodologies, they can be divided into different types of immunoreagents, such as agglutination reaction, precipitation reaction, complement fixation reaction and labeled immune reaction.

[0077] Alternatively, all process combination results can be determined by enumeration and then grouped as the multiple process combination results described above. Alternatively, multiple process combination results can be determined in a customized manner. For example, multiple process combination results required for experiments can be determined based on actual reagent development requirements.

[0078] S2. Sort the results of multiple process combinations as the experimental sequence, set the experimental sequence, and conduct experiments on the results of each process combination according to the experimental sequence;

[0079] In the embodiment of the present invention, a certain order is used as an experimental sequence based on the results of the above-determined combination of multiple processes, and the experimental results of multiple immune reagents are obtained by performing experiments in this experimental sequence.

[0080] Optionally, each process combination result includes multiple sub-processes, including adding sample, adding diluent, diluting and mixing, adding reagent, etc. The embodiment of the present invention can set the final process combination result according to actual reagent development requirements to generate multiple immune reagents.

[0081] S3. After the experiment is completed, the experimental results of multiple immune reagents are output, and according to the performance test results of each immune reagent, a target immune reagent that meets the preset conditions is determined from the multiple immune reagents.

[0082] In an embodiment of the present invention, after obtaining multiple immunoassays, each immunoassay needs to be performance-verified. Based on the performance test results of each immunoassay, a target immunoassay that meets preset conditions is determined from the multiple immunoassays. Meeting the preset conditions indicates that the performance verification is qualified. For example, the performance verification criteria may be international standards or other criteria set forth in other standards, and may be determined based on actual circumstances.

[0083] Furthermore, the process combination results corresponding to the target immune reagent are stored in the IC card so that the detection instrument can read and execute them repeatedly in subsequent tests, thereby meeting different reagent development requirements.

[0084] In one embodiment, based on the type of immunoassay to be developed, multiple process combination results are determined, including:

[0085] According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed;

[0086] The processes in each process set are arranged and combined to obtain multiple process combination results.

[0087] In an embodiment of the present invention, after determining a target immunoreagent that meets the preset conditions from a plurality of immunoreagents, the process combination results of the target immunoreagent are stored in an IC card by writing to the IC card so that the detection instrument can repeatedly read and execute them in subsequent detections, thereby meeting different reagent development requirements. In one embodiment, determining a target immunoreagent that meets the preset conditions from a plurality of immunoreagents based on the performance test results of each immunoreagent includes:

[0088] S31, obtaining response values of multiple samples with known concentrations;

[0089] In an embodiment of the present invention, the process combination result includes process parameters and reagent information required for the experiment. According to the position information corresponding to the reagent information, the position of the reagent inside the detection instrument can be accurately obtained, so that the reagent can be quickly called, and the reaction values of multiple samples of known concentrations can be detected in combination with the reagent and the preset process parameters.

[0090] S32, fitting the response values and parameter values of a plurality of samples with known concentrations into a fitting curve;

[0091] In an embodiment of the present invention, the parameter values and reaction values of samples of known concentration are taken as a set of data groups. Based on the linear relationship between the parameter values and the reaction values, a preset fitting algorithm is used to fit the multiple data groups into a fitting curve to facilitate subsequent analysis of the parameter values of the immune reagent, wherein the preset fitting algorithm includes a logistic fitting algorithm, and the parameter values include concentration.

[0092] S33, substituting the response value of each immune reagent into the fitting curve to obtain the parameter value of the immune reagent;

[0093] The fitting curve represents the linear relationship between the reaction value and the parameter value. In the embodiment of the present invention, the reaction value of the immune reagent is substituted into the fitting curve to estimate the parameter value of the immune reagent.

[0094] S34. When the parameter value of the immune reagent meets the preset condition, determine that the immune reagent meets the preset condition.

[0095] In one embodiment, when the parameter value of the immune reagent meets the preset condition, determining that the immune reagent meets the preset condition includes:

[0096] Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds the preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset conditions, and determine that the immune reagent is correct, and generate the corresponding project file for its corresponding process combination result and store it so that the detection instrument can call the process combination result.

[0097] In an embodiment of the present invention, the preset standard parameter value is the standard parameter value of the immune reagent. The process combination result and the data of the known concentration sample are stored in an evaluation file and named. The user can judge the process combination result by clicking the evaluation file with the corresponding name in the evaluation list. When the deviation value between the standard parameter value of the immune reagent and the parameter value obtained by the immune reagent test does not exceed the preset threshold range, the immune reagent is judged to be correct. At this time, the corresponding process combination result is stored on the IC card so that the detection instrument can read and execute the process combination result, which is conducive to improving the convenience of subsequent development of such reagents.

[0098] Furthermore, after determining that the immunoassay reagent meets the preset conditions, the embodiment of the present invention combines the process combination result and the fitting curve corresponding to the immunoassay reagent to generate a project file in HEX format, and stores the project file on an IC card so that it can be repeatedly read by the detection instrument.

[0099] It should be noted that after the immune reagent meets the preset conditions, the embodiment of the present invention also determines the correctness of the process combination result by whether the data can be loaded correctly. For example, it receives the data information selected by the user and determines whether the user's selected data information can be loaded on the display interface, including the test peak data and curve of the concentration. If it can be loaded correctly, it is determined that the process combination result of the embodiment of the present invention is correct. If it cannot be loaded correctly, it is determined that there is a problem with the process combination result of the embodiment of the present invention. At this time, it is necessary to redesign the process by adjusting the configuration parameters and re-determine whether the process combination result is correct.

[0100] Furthermore, embodiments of the present invention can also repeat multiple tests to obtain parameter values for multiple immunoassays, and then determine whether these immunoassays meet preset conditions based on the performance tests of the multiple immunoassays, thereby further improving the accuracy and reliability of immunoassay development. As a specific implementation of an embodiment of the present invention, after detecting and obtaining parameter values for more than four immunoassays, the embodiment of the present invention further determines whether the immunoassay meets the preset conditions.

[0101] In one embodiment, obtaining response values of a plurality of samples of known concentrations includes:

[0102] According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores the reagent for detecting the reaction value of the sample with known concentration. If so, the reagent is called and the reaction values of multiple samples with known concentration are obtained using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

[0103] In an embodiment of the present invention, if the detection instrument does not store the configured reagent for detecting the reaction value of a sample with a known concentration, a reminder message is generated to remind the user to reconfigure the reagent. After receiving the actual information of the user's reconfiguration, it is re-determined whether the instrument contains the configured reagent for detecting the reaction value of a known concentration, until the configured reagent can be detected in the detection instrument, so as to use the reagent and the configured process to detect the reaction values of multiple samples with known concentrations.

[0104] In one embodiment, fitting the response values and parameter values of a plurality of samples of known concentrations into a fitting curve comprises:

[0105] The response value and parameter value of each sample with known concentration are combined into a data group;

[0106] Use a preset fitting algorithm to fit multiple data sets into a fitting curve.

[0107] In an embodiment of the present invention, the preset fitting algorithms include a logistic fitting algorithm, a least squares method, etc., and the fitting result outputs a fitting curve and a correlation coefficient R, wherein the correlation coefficient R is used to determine whether the fitting curve is reliable.

[0108] In one embodiment, the response value and parameter value of each sample with known concentration are grouped into a data set, including:

[0109] After the parameter value and response value of each sample with known concentration are transformed into logarithms, a data group is formed;

[0110] Or, after setting the number of decimal places for the parameter value and reaction value of each sample with known concentration, a data group is formed.

[0111] In an embodiment of the present invention, in order to improve the accuracy of the fitting curve, the reaction values and parameter values of the known concentration sample can be preprocessed before fitting to the fitting curve. The preprocessing includes converting the data into a logarithmic form and setting the number of decimal places of the data. Exemplarily, converting the data into a logarithmic form includes: taking the logarithm with base 10, taking the logarithm with base natural numbers, taking the logarithm with base 2, etc.; setting the number of decimal places of the data includes: setting the number of decimal places to 1, setting the number of decimal places to 2, setting the number of decimal places to 3, etc.

[0112] In one embodiment, before substituting the reaction values of the respective immune reagents into the fitting curve to obtain the parameter values of the immune reagents, the method further includes:

[0113] Set sampling parameters according to the detection requirements of parameter value detection;

[0114] In an embodiment of the present invention, the sampling parameters include a sampling interval, a total sampling time, and a single sampling time, wherein the sampling interval is set to correspond to the total sampling time. When the total sampling time is long, the sampling interval needs to be set relatively long; when the total sampling time is short, the sampling interval needs to be set relatively short.

[0115] collecting multiple raw reaction values of the immunoreagent during the detection process according to the sampling parameters;

[0116] It is understandable that after the detection is completed, multiple original reaction values will be collected. These original reaction values are the reaction values of the immune reagent at different collection times. In order to improve the accuracy of the reaction value detection, the embodiment of the present invention selects a luminescence curve suitable for the experiment from the peak curve drawn from all the original reaction values, and further determines the reaction value of the immune reagent based on the multiple reaction values in the luminescence curve. For example, the result of a complete reflection value process combination is 30 seconds, and the peak curve reflects the change in the reaction value of the complete process combination result for 30 seconds. However, in actual detection applications, the luminescence curve suitable for the experiment is from the 10th second to the 15th second from the start of the detection. The embodiment of the present invention determines to select the curve from the 10th second to the 15th second in the complete peak curve as the luminescence curve by calculating the starting point and end point of this section of the luminescence curve. Among them, the parameters for calculating the starting point and the calculation end point can be input by the user through the display interface.

[0117] Plotting multiple original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve;

[0118] In an embodiment of the present invention, a section of the luminescence curve in the luminescence cycle is selected from the peak curve for experimental analysis. The selection method is to determine the starting time and the ending time of the luminescence curve through historical detection data, or directly select based on the experience of the detection personnel.

[0119] The average of several raw response values in the luminescence curve was taken as the response value of each immunoreagent.

[0120] In the embodiment of the present invention, the luminescence curve includes several original reaction values, and the reaction value of the immunoreagent obtained by the final test is determined based on these original reaction values by designing a calculation method, and the calculation method includes integration method and average method.

[0121] In the embodiment of the present invention, there are many calculation methods for determining the reaction value of the immunoassay, including an average method, an integration method, and the like.

[0122] In one embodiment, before determining the results of combining multiple processes based on the type of immunoassay to be developed, the reagent development experimental method further includes:

[0123] Acquire the position information of multiple reagents inside the detection instrument, and match the position information of the multiple reagents with the corresponding reagent information.

[0124] In an embodiment of the present invention, a plurality of reagents are disposed within the detection instrument, each of which has corresponding reagent information, including the reagent name, such as Reagent A, Reagent B, Reagent C, etc. Each reagent disposed within the detection instrument occupies internal space of the detection instrument. In an embodiment of the present invention, a coordinate system is constructed with a certain point within the detection instrument as the origin, and the position information of each reagent is represented by coordinates. The position information of the reagent is then matched with the reagent information. When a reagent is required based on the subsequent process combination results, the required reagent can be quickly and conveniently called based on the position information.

[0125] The implementation of the present invention has the following beneficial effects:

[0126] The embodiment of the present invention determines multiple process combination results based on the type of immune reagent to be developed, sorts the multiple process combination results as an experimental sequence, conducts experiments on each process combination result according to the experimental sequence to obtain multiple immune reagents, and then determines a target immune reagent that meets the preset conditions by performing performance tests on each immune reagent. The embodiment of the present invention automatically conducts experiments using the predetermined multiple process combination results as the experimental sequence, and can quickly generate multiple immune reagents corresponding to different process combination results. The embodiment of the present invention automatically detects the performance of each immune reagent to determine the target immune reagent that meets the preset conditions, which can not only effectively improve the efficiency of reagent development, but also effectively reduce the human errors caused by the human development of reagents, thereby effectively improving the reliability of reagent development.

[0127] Furthermore, in the embodiment of the present invention, the position information of the reagent required for the development of the immune reagent in the detection instrument matches the reagent information. The position information of the reagent can be determined according to the configured reagent information. The detection instrument can directly call the reagent according to the position information during the detection process, which is beneficial to improving the efficiency of reagent development; on the other hand, the configuration of the reagent information and the reagent calling information can be recorded. By recording the information, the use of the reagent on the detection instrument can be monitored, and adjustments can be made according to the use of the reagent, which is beneficial to the convenience of reagent development and thus helps to meet different reagent development needs.

[0128] See also Figure 3 Based on the same technical concept as the above embodiment, one embodiment of the present invention provides a reagent development experimental device, comprising:

[0129] A process combination determination module 10 is used to determine multiple process combination results according to the type of immune reagent to be developed;

[0130] The experiment module 20 is used to sort the results of multiple process combinations as an experiment sequence, set the experiment sequence, and conduct experiments on the results of each process combination according to the experiment sequence;

[0131] The reagent determination module 30 is used to output the experimental results of multiple immune reagents after the experiment is completed, and determine the target immune reagent 30 that meets the preset conditions from the multiple immune reagents based on the performance test results of each immune reagent.

[0132] In one embodiment, the process combination determination module 10 is configured to:

[0133] According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed;

[0134] The processes in each process set are arranged and combined to obtain multiple process combination results.

[0135] See also Figure 4 In one embodiment, the reagent determination module 30 includes:

[0136] A known concentration sample reaction value detection unit 310 is used to obtain reaction values of multiple known concentration samples;

[0137] A fitting unit 320 is used to fit the reaction values and parameter values of a plurality of samples with known concentrations into a fitting curve;

[0138] The parameter value acquisition unit 330 is used to substitute the reaction value of each immune reagent into the fitting curve to obtain the parameter value of the immune reagent;

[0139] The immune reagent determination unit 340 is configured to determine whether the immune reagent meets the preset condition when the parameter value of the immune reagent meets the preset condition.

[0140] In one embodiment, the immune reagent determination unit 340 is specifically configured to:

[0141] Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds the preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset condition, and determine that the immune reagent meets the preset condition.

[0142] In one embodiment, the known concentration sample response value detection unit 310 is used to:

[0143] According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores the reagent for detecting the reaction value of the sample with known concentration. If so, the reagent is called and the reaction values of multiple samples with known concentration are obtained using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

[0144] In one embodiment, the fitting unit 320 is specifically configured to:

[0145] The response value and parameter value of each sample with known concentration are combined into a data group;

[0146] Use a preset fitting algorithm to fit multiple data sets into a fitting curve.

[0147] In one embodiment, the response value and parameter value of each sample with known concentration are grouped into a data set, including:

[0148] After transforming the parameter value and response value of each sample with known concentration into logarithms, form a data set; or

[0149] After setting the number of decimal places for the parameter value and reaction value of each sample with known concentration, a data group is formed.

[0150] In one embodiment, the parameter value obtaining unit 330 is configured to:

[0151] Set sampling parameters according to the detection requirements of parameter value detection;

[0152] collecting multiple raw reaction values of each immunoreagent during the detection process according to sampling parameters;

[0153] Plotting multiple original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve;

[0154] The average of several raw response values in the luminescence curve was taken as the response value of each immunoreagent.

[0155] In one embodiment, the reagent development experimental device further includes an information matching module for:

[0156] Acquire the position information of multiple reagents inside the detection instrument, and match the position information of the multiple reagents with the corresponding reagent information.

[0157] One embodiment of the present invention provides a terminal, including a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, the above-mentioned reagent development experimental method is implemented.

[0158] One embodiment of the present invention provides a computer-readable storage medium, which includes a stored computer program; wherein, when the computer program is run, it controls the device where the computer-readable storage medium is located to execute the reagent development experimental method as described above.

[0159] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A reagent development experimental method, characterized in that, include: Determine the results of multiple process combinations based on the type of immunoassay to be developed; sorting the plurality of process combination results as an experimental sequence, setting the experimental sequence, and conducting experiments on the process combination results according to the experimental sequence; After the experiment is completed, the experimental results of multiple immune reagents are output, and according to the performance test results of each immune reagent, a target immune reagent that meets the preset conditions is determined from the multiple immune reagents; Wherein, the determination of multiple process combination results according to the type of immune reagent to be developed includes: According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed; The processes in each of the process sets are arranged and combined to obtain multiple process combination results.

2. The reagent development experimental method according to claim 1, characterized in that The step of determining a target immune reagent that meets preset conditions from a plurality of immune reagents based on the performance test results of each immune reagent includes: Obtain response values for multiple samples of known concentrations; Fitting the response values and parameter values of the plurality of samples with known concentrations into a fitting curve; Substituting the reaction value of each of the immune reagents into the fitting curve to obtain the parameter value of the immune reagent; When the parameter value of the immune reagent meets the preset condition, it is determined that the immune reagent meets the preset condition.

3. The reagent development experimental method according to claim 2, characterized in that: When the parameter value of the immune reagent meets the preset condition, determining that the immune reagent meets the preset condition includes: Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds a preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset condition, and determine that the immune reagent meets the preset condition.

4. The reagent development experimental method according to claim 2, characterized in that: The setting of the experimental sequence and conducting experiments on the results of each process combination according to the experimental sequence includes: According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores a reagent for detecting the reaction value of a sample with a known concentration. If so, the reagent is called, and the reaction values of multiple samples with a known concentration are obtained using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

5. The reagent development experimental method according to claim 2, wherein: The step of fitting the reaction values and parameter values of the plurality of samples with known concentrations into a fitting curve comprises: Combining the response value and parameter value of each sample of known concentration into a data set; The plurality of data sets are fitted into a fitting curve using a preset fitting algorithm.

6. The reagent development experimental method according to claim 5, characterized in that: The step of forming a data set of the reaction value and parameter value of each sample of known concentration includes: Convert the parameter value and the response value of each sample of known concentration into logarithms to form a data set; or After setting the number of decimal places for the parameter value and the reaction value of each sample of known concentration, a data group is formed.

7. The reagent development experimental method according to claim 2, wherein: Before substituting the reaction values of the respective immune reagents into the fitting curve to obtain the parameter values of the immune reagents, the method further includes: Set sampling parameters according to the detection requirements of parameter value detection; Collecting multiple original reaction values of each of the immunoreagents during the detection process according to the sampling parameters; Plotting a plurality of the original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve; The average of several original reaction values in the luminescence curve is taken as the reaction value of each immunoreagent.

8. The reagent development experimental method according to claim 1, wherein Before determining the results of multiple process combinations based on the type of immunoassay to be developed, it also includes: The position information of multiple reagents inside the detection instrument is obtained, and the position information of the multiple reagents is matched with the corresponding reagent information.

9. A reagent development experimental device, characterized in that: include: A process combination determination module is used to determine multiple process combination results according to the type of immune reagent to be developed; An experiment module, configured to sort the plurality of process combination results into an experiment sequence, set the experiment sequence, and conduct experiments on each of the process combination results according to the experiment sequence; a reagent determination module, configured to output the experimental results of multiple immune reagents after the experiment is completed, and determine a target immune reagent that meets preset conditions from the multiple immune reagents based on the performance test results of each immune reagent; Wherein, the process combination determination module is used to: According to the type of the immunoreagent to be developed, the processes required for the immunoreagent to be developed are divided to obtain multiple process sets, each process set corresponding to a type of immunoreagent to be developed; The processes in each of the process sets are arranged and combined to obtain multiple process combination results.

10. The reagent development experimental device according to claim 9, characterized in that: The reagent determination module includes: A known concentration sample reaction value detection unit is used to obtain reaction values of multiple known concentration samples; A fitting unit, configured to fit the reaction values and parameter values of the plurality of samples of known concentrations into a fitting curve; a parameter value acquisition unit, configured to substitute the reaction value of each of the immune reagents into the fitting curve to acquire the parameter value of the immune reagent; The immune reagent judging unit is used to judge whether the immune reagent meets the preset condition when the parameter value of the immune reagent meets the preset condition.

11. The reagent development experimental device according to claim 10, characterized in that: The immune reagent judgment unit is specifically used to: Determine whether the deviation between the parameter value of the immune reagent and the preset standard parameter value exceeds a preset threshold range. If not, determine that the parameter value of the immune reagent meets the preset condition, and determine that the immune reagent meets the preset condition.

12. The reagent development experimental device according to claim 10, characterized in that: The known concentration sample reaction value detection unit is used for: According to the location information corresponding to the reagent information, it is determined whether the detection instrument stores a reagent for detecting the reaction value of a sample with a known concentration. If so, the reagent is called, and the reaction values of multiple samples with a known concentration are obtained by using the reagent and the configured process parameters; if not, a reminder message is generated to remind the user to reconfigure the reagent information.

13. The reagent development experimental device according to claim 10, wherein: The fitting unit is specifically used for: Combining the response value and parameter value of each sample of known concentration into a data set; The plurality of data sets are fitted into a fitting curve using a preset fitting algorithm.

14. The reagent development experimental device according to claim 13, wherein: The step of forming a data set of the reaction value and parameter value of each sample of known concentration includes: Convert the parameter value and the response value of each sample of known concentration into logarithms to form a data set; or After setting the number of decimal places for the parameter value and the reaction value of each sample of known concentration, a data group is formed.

15. The reagent development experimental device according to claim 10, wherein: Parameter value acquisition unit, used to: Set sampling parameters according to the detection requirements of parameter value detection; Collecting multiple original reaction values of each of the immunoreagents during the detection process according to the sampling parameters; Plotting a plurality of the original reaction values into a peak curve, and selecting a section of the luminescence curve from the peak curve; The average of several original reaction values in the luminescence curve is taken as the reaction value of each immunoreagent.

16. The reagent development experimental device according to claim 9, characterized in that: It also includes an information matching module for: The position information of multiple reagents inside the detection instrument is obtained, and the position information of the multiple reagents is matched with the corresponding reagent information.

17. A terminal comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein: When the processor executes the computer program, the reagent development experimental method according to any one of claims 1 to 8 is implemented.

18. A computer-readable storage medium, characterized in that The computer-readable storage medium includes a stored computer program; wherein, when the computer program is run, it controls the device where the computer-readable storage medium is located to execute the reagent development experimental method according to any one of claims 1 to 8.

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