Vitamin electrochemical detection chip based on finger pressure pump and preparation method

Abstract

The present invention relates to the field of chip technology, disclosing a vitamin electrochemical detection chip based on a finger pressure pump and its preparation method. The chip comprises an electrode slot layer, a microstructure layer, and a sealing film layer, each of which is provided in sequence. The electrode slot layer houses electrodes; the electrodes comprise a base layer, a silver lead / silver electrode layer, a carbon lead / main electrode layer, and an insulating lacquer layer, each of which is provided in sequence. The present invention is primarily used for rapid vitamin detection and integrates automatic sampling, mixing, and electrochemical detection, achieving efficient detection of small sample volumes.

Description

Technical Field

[0001] The present invention relates to the technical field of chips, and in particular to a vitamin electrochemical detection chip based on a finger pressure pump and a preparation method thereof. Background Art

[0002] Vitamins are a group of organic compounds that are essential trace nutrients for living organisms. They are generally not produced by organisms themselves and must be obtained through diet and other means. Unlike carbohydrates, proteins, and fats, vitamins cannot generate energy or form cells, but they do regulate metabolism. Vitamin deficiency can lead to serious health problems; adequate intake can maintain good health; excessive intake can lead to toxicity.

[0003] The discovery and application of vitamins have been around for nearly 150 years, but the detection of vitamins currently relies mainly on laboratory testing. The instrumental detection methods currently used for vitamin detection mainly include high performance liquid chromatography, high performance liquid chromatography-mass spectrometry, capillary electrophoresis, fluorescence spectroscopy, enzyme-linked immunosorbent assay, time-resolved fluorescence, electrochemiluminescence and other methods. However, the above methods currently have complex detection procedures and long test cycles, which limit their use in rapid detection environments such as homes. Therefore, it is of great significance to develop a simple, fast, and low-cost vitamin detection device. Based on this, the present invention aims to develop a chip that can realize the functions of automatic sampling, mixing and electrochemical detection to simplify the detection process of vitamins. Summary of the Invention

[0004] The present invention aims to provide a vitamin electrochemical detection chip based on a finger pressure pump and a preparation method thereof, so as to realize automatic sampling, mixing and electrochemical detection of the chip.

[0005] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: a vitamin electrochemical detection chip based on a finger pressure pump, comprising an electrode slot layer, a microstructure layer and a sealing film layer arranged in sequence, wherein an electrode is installed in the electrode slot layer; the electrode comprises a base layer, a silver lead / silver electrode layer, a carbon lead / main electrode layer and an insulating paint layer arranged in sequence; a detection chamber, a connecting channel and a finger pressure pump are provided on the microstructure layer, and a vent is provided on the connecting channel.

[0006] On the other hand, the present technical solution provides a method for preparing a vitamin electrochemical detection chip based on a finger pressure pump, characterized in that it comprises the following steps:

[0007] Step 1: Prepare the electrodes. Prepare a base layer and use a screen printing process to print a conductive silver paste on the base layer to form an auxiliary electrode, a reference electrode, a working electrode silver base, a lead silver base, and a finger electrode slot silver base; continue to use a screen printing process to print a conductive carbon paste on the base layer to form a working electrode carbon surface, a finger electrode slot carbon surface, and a lead carbon surface; then use a screen printing process to print a UV-curable insulating ink on the base layer to obtain an insulating paint layer, and the electrode preparation is completed;

[0008] Step 2: Prepare the electrode slot. The electrode slot is prepared by polydimethylsiloxane inverting mold. The mold used is made by 3D printing. The 3D printing mold material is light-curing resin.

[0009] Step 3: preparing a microstructure layer by casting polydimethylsiloxane in a mold made by 3D printing;

[0010] Step 4: Prepare a sealing film layer made of polyethylene film to close and open the vents;

[0011] Step 5: Assembling: After inserting the electrode into the electrode slot, the electrode slot layer and the microstructure layer are assembled through a bonding process to obtain a vitamin electrochemical detection chip based on a finger pressure pump.

[0012] The principles and advantages of this solution are: in actual application, screen-printed electrodes are low-cost, but the common devices using screen-printed electrodes as sensors are all used by directly dropping the detection object on the electrode surface. In this way, when the volume of the solution is small (<40μL), it is difficult to make the solution cover the entire surface of the electrode detection part, which reduces the effective area of ​​the electrode and affects the detection effect; and the detection directly dripping on the electrode surface is prone to sample contamination and evaporation. Based on this, the present technical solution considers combining screen-printed electrodes with microfluidic chips to achieve efficient detection of small volume (<40μL) samples and avoid sample contamination and evaporation. Moreover, when detecting vitamins, unlike other target substances, it is necessary to maintain a neutral detection environment by adding buffer. For this reason, the inventors integrated the detection electrode into the microfluidic chip to achieve on-chip mixing of the sample and buffer, simplifying the sample processing steps. During the early experiments, due to the through-type chip structure, the negative pressure generated by the finger pressure pump would cause the sample to directly enter the finger pressure pump and fail to fill the entire detection chamber; even if the finger pressure pump and the detection chamber were combined into one chamber, the detection chamber would still fail to be filled, affecting the detection results. Based on this, the inventors continued to optimize the chip structure, setting an air vent on the detection channel. The air vent acts as a shut-off valve. After the sample fills the detection chamber, the air vent is opened to balance the air pressure in the finger pressure pump, which can produce the effect of a shut-off valve and prevent the sample from entering the detection chamber; then the finger-shaped interface of the chip is connected to the electrochemical workstation through a customized interface to perform electrochemical detection on the sample. This technical solution realizes the automatic sampling, mixing and electrochemical detection of vitamins, is easy to operate, and has a very high prospect for promotion and application; and realizes efficient detection of small-volume samples. From the experimental results, the electrochemical response of Vc detected on the chip is higher than that of direct detection using screen-printed electrodes.

[0013] Preferably, as an improvement, an electrode slot for installing the electrode is provided in the electrode slot layer, and the electrode slot layer is made of polydimethylsiloxane.

[0014] In this technical solution, since the dimensions of the electrode slots and electrodes cannot be completely consistent, some deviations are inevitable during actual processing. However, the elasticity of polydimethylsiloxane (PDMS) can largely eliminate the impact of these deviations. If harder materials such as PMMA are used, these deviations cannot be eliminated, affecting the sealing of the entire device. In addition, the microstructure layer in this technical solution is also made of polydimethylsiloxane (PDMS), which also facilitates bonding with the microstructure layer.

[0015] Preferably, as an improvement, the microstructure layer is prepared by casting polydimethylsiloxane in a mold produced by 3D printing, and the microstructure layer is also provided with an injection port, a buffer port and a mixing structure, and the mixing structure is connected to the detection chamber through a connecting channel.

[0016] In this technical solution, the injection port and the buffer port are used for the injection of samples and buffer respectively. After mixing, they enter the detection chamber through the connecting channel for detection, and the structural design is reasonable. Traditional molds are made using soft lithography technology. Single-layer soft lithography can only etch a height of no more than 300μm, and it is impossible to produce structures of various heights, and multi-layer soft lithography is very difficult to operate. The channel height range used in this solution is 200μm-1000μm, which cannot be achieved using soft lithography. Therefore, in this technical solution, the mold is made using 3D printing, which can make the size of the channel structure more flexible, and 3D printing is simple to operate. Structures of different heights can be printed at one time, which is low cost and has more advantages than lithography.

[0017] Preferably, as an improvement, the sealing film layer is made of polyethylene film.

[0018] In this technical solution, the sealing film primarily serves to seal and release vents. Its primary requirement is that it can be repeatedly torn and reattached without compromising the sealing effect. Polyethylene (PE) film can meet these requirements. Furthermore, in actual applications, other materials that meet these requirements can be substituted based on actual circumstances.

[0019] Preferably, as an improvement, the base layer of the electrode is made of polyethylene terephthalate.

[0020] In this technical solution, the base layer of the electrode is made of polyethylene terephthalate, which is a suitable choice that has been verified in practice and can meet processing requirements.

[0021] Preferably, as an improvement, the silver lead / silver electrode layer is arranged on the base layer, and the silver lead / silver electrode layer includes an auxiliary electrode, a reference electrode, a working electrode silver base, a lead silver base, and a finger electrode slot silver base.

[0022] In this technical solution, the silver paste layer is mainly used to enhance its conductivity and form a reference electrode and an auxiliary electrode. The above-mentioned base layer structure can meet the processing and detection requirements.

[0023] Preferably, as an improvement, the carbon lead / main electrode layer is provided on the base layer, and the carbon lead / main electrode layer includes a working electrode carbon surface, a finger electrode slot carbon surface, and a lead carbon surface.

[0024] In this technical solution, the above structure mainly forms a working electrode, thereby ensuring effective detection in the later stage.

[0025] Preferably, as an improvement, the insulating paint layer is formed by printing ultraviolet light curing insulating ink or heat curing insulating ink on the base layer using a screen printing process.

[0026] In this technical solution, the screen printing technology has low cost and high precision, and the entire electrode is made by the screen printing process, which is more convenient and quick.

[0027] Preferably, as an improvement, the mold making method in step 2 and step 3 is:

[0028] (1) Design the 3D drawing of the mold using CAD and SolidWorks;

[0029] (2) Importing the 3D image in (1) into a 3D printer through Anycubic Photon Workshop;

[0030] (3) After printing, clean it with alcohol ultrasonically;

[0031] (4) Use ultraviolet light curing;

[0032] (5) Bake on a hot plate at 120°C for 1-2 hours;

[0033] (6) Plasma cleaning;

[0034] (7) Spin-coat a release agent on the treated surface of the mold to obtain the mold.

[0035] Before 3D printing, electrode slots could not be fabricated using photolithography (they were too high). To integrate the electrodes into the chip, attempts were made to place the electrodes face down at the bottom of a homemade glass mold. Polydimethylsiloxane (PDMS) was then poured into the mold to cure. After peeling the electrodes off, the PDMS film on the electrode surface was removed to bond them to the structural layer. However, this preparation method damaged the electrodes, making it impossible for the chip to detect Vc. In this technical solution, 3D printing technology is used to produce relatively large structures. 3D printing is also simple to operate, and structures of varying heights can be printed in a single step, offering significant advantages over photolithography. After printing, alcohol ultrasonic cleaning is primarily used to remove residual resin from the mold. Baking is used to remove photoinitiators from the mold (photoinitiators inhibit the curing of PDMS). Too low a temperature will not achieve this removal effect, while too high a temperature can cause the mold to crack. Plasma cleaning allows for better adhesion of the release agent. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the structure of the vitamin electrochemical detection chip based on the finger pressure pump in an embodiment of the present invention.

[0037] Figure 2 Schematic diagram of the structure of the electrode in an embodiment of the present invention.

[0038] Figure 3Schematic diagram of the structure of the silver lead / silver electrode layer in an embodiment of the present invention.

[0039] Figure 4 Schematic diagram of the structure of the carbon lead / main electrode layer in an embodiment of the present invention.

[0040] Figure 5 This is the assembly diagram of the electrode and detection chip.

[0041] Figure 6 This is a diagram showing the detection results of treatment group 1 in Experimental Example 1 of the present invention.

[0042] Figure 7 This is a diagram showing the detection results of treatment group 2 in Experimental Example 1 of the present invention. DETAILED DESCRIPTION

[0043] The following is further described in detail through specific embodiments, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art; the experimental methods used are all conventional methods; and the materials, reagents, etc. used are all commercially available.

[0044] The figure marks in the drawings of the specification include: electrode slot layer 1, microstructure layer 2, sealing film layer 3, electrode 4, base layer 5, silver lead / silver electrode layer 6, carbon lead / main electrode layer 7, insulating paint layer 8, auxiliary electrode 9, reference electrode 10, working electrode silver substrate 11, lead silver substrate 12, finger electrode slot silver substrate 13, working electrode carbon surface 14, finger electrode slot carbon surface 15, lead carbon surface 16, lead area 17, injection port 18, buffer port 19, connecting channel 20, mixing structure 21, detection chamber 22, vent 23, finger pressure pump 24.

[0045] Example 1

[0046] The embodiment is basically as shown in the attached Figure 1 As shown: A vitamin electrochemical detection chip based on a finger pressure pump, which includes an electrode slot layer 1, a microstructure layer 2, and a sealing film layer 3 from the bottom layer upward.

[0047] The electrode slot layer 1 is made of polydimethylsiloxane (PDMS), 50mm long, 26mm wide and 2mm thick. The size can be adjusted appropriately according to the actual use. The electrode slot layer 1 is provided with an electrode slot. The electrode slot is 15.25mm long, 7.1mm wide and 0.36mm thick. The electrode slot is used to fix the electrode 4 in the chip. The size can be adjusted appropriately according to the actual use. In this embodiment, the electrode slot is prepared by polydimethylsiloxane (PDMS) inverted molding, and the mold used is made by 3D printing; combined with Figure 2As shown, the electrode 4 includes a base layer 5, a silver lead / silver electrode layer 6, a carbon lead / main electrode layer 7, and an insulating paint layer 8 arranged from bottom to top. Among them, the base layer 5 is made of polyethylene terephthalate (PET). In this embodiment, the base layer 5 has a width of 7 mm, a length of 40 mm, and a thickness of 0.35 mm. In actual use, the size can be appropriately adjusted according to actual needs. The silver lead / silver electrode layer 6 is prepared by screen printing technology, and conductive silver paste is printed on the base layer 5. An auxiliary electrode 9, a reference electrode 10, a working electrode silver substrate 11, a lead silver substrate 12, and a finger electrode slot silver substrate 13 (such as Figure 3 As shown). The carbon lead / main electrode layer 7 is prepared by printing a conductive carbon paste on the base layer 5 using a screen printing process. The working electrode carbon surface 14, the finger electrode slot carbon surface 15, and the lead carbon surface 16 (as shown) are formed on the base layer 5. Figure 4 The insulating varnish layer 8 is formed by screen printing a UV-curable insulating ink on the lead area 17 of the base layer 5. The purpose is to achieve electrical insulation and avoid interference, error or short circuit during the detection process.

[0048] Combine Figure 5 As shown, the microstructure layer 2 is prepared by casting polydimethylsiloxane (PDMS) in a 3D-printed mold. It is 50 mm long, 26 mm wide, and 3 mm thick. The microstructure layer 2 is provided with an injection port 18, a buffer port 19, a connecting channel 20, a mixing structure 21, a detection chamber 22, a vent 23, and a finger pressure pump 24. The injection port 18 is a through hole with a diameter of 2 mm; the buffer port 19 is a through hole with a diameter of 2 mm; the connecting channel 20 is 0.2 mm wide and 0.2 mm high; the mixing structure 21 is connected to the detection chamber 22 through the connecting channel 20, and the mixing structure 21 is 0.2 mm wide and 0.2 mm high; the detection chamber 22 is 10 mm in diameter and 0.2 mm in height; the vent 23 is a through hole with a diameter of 2 mm; and the finger pressure pump 24 is arranged on the left side of the detection chamber 22, and the finger pressure pump 24 is 20 mm in diameter and 0.2 mm high.

[0049] The sealing film layer 3 is made of polyethylene (PE) film and is a 10 mm x 10 mm rectangle, and is used to seal the vent holes 23 in the microstructure layer 2 .

[0050] A method for preparing a vitamin electrochemical detection chip based on a finger pressure pump, which is prepared using micromachining technology and a printed electrode process, specifically comprising the following steps:

[0051] Step 1: Prepare the electrode and prepare a polyethylene terephthalate (PET) base layer with a width of 7 mm, a length of 40 mm, and a thickness of 0.35 mm. Use the screen printing process to print the conductive silver paste on the base layer to form an auxiliary electrode, a reference electrode, a working electrode silver base, a lead silver base, and a finger electrode slot silver base; continue to use the screen printing process to print the conductive carbon paste on the base layer to form a working electrode carbon surface, a finger electrode slot carbon surface, and a lead carbon surface; then use the screen printing process to print UV-curable insulating ink on the base layer to obtain an insulating paint layer, and the electrode preparation is completed.

[0052] Step 2: Prepare the electrode slot. The electrode slot is prepared by polydimethylsiloxane (PDMS) mold. The mold used is made by 3D printing. The 3D printing mold material is light-curing resin. The mold is made and processed as follows:

[0053] (1) Design the 3D drawing of the mold using CAD and SolidWorks;

[0054] (2) Importing the 3D image in (1) into a 3D printer through Anycubic Photon Workshop;

[0055] (3) After printing, ultrasonic cleaning was performed with 95% alcohol for 10 min;

[0056] (4) Use UV light to cure for 1 hour;

[0057] (5) Bake on a hot plate at 120°C for 2 hours;

[0058] (6) Plasma cleaning 180S;

[0059] (7) Spin-coating a release agent on the treated mold surface; the prepared mold has a bottom thickness of 2 mm, a groove depth of 3 mm, and a groove edge thickness of 2 mm.

[0060] Step 3: Prepare the microstructure layer. The microstructure layer is prepared by using polydimethylsiloxane (PDMS) to cast in a mold made by 3D printing. The material and preparation process of the 3D printing mold are the same as those in step 2.

[0061] Step 4: Prepare a sealing film layer. The material used for the sealing film layer is a polyethylene (PE) film with dimensions of 1 cm in length, 1 cm in width, and 50 μm in thickness. The sealing film layer is used to close and open the vents.

[0062] Step five, assembly, after inserting the electrode into the electrode slot, assemble the electrode slot layer and the microstructure layer through a bonding process to obtain a vitamin electrochemical detection chip based on a finger pressure pump. Among them, bonding consists of two parts: plasma bonding and thermal bonding. Plasma bonding: Use an oxygen plasma cleaning machine to modify the bonding surface of the prepared polydimethylsiloxane (PDMS) structure for 5-15s. Since the surface modification state lasts for a short time, the two pieces of polydimethylsiloxane (PDMS) must be bonded within 1-10 minutes after the treatment is completed. Thermal bonding: Place the plasma-bonded structure on a hot plate at 90-150°C and bake for 10-30 minutes. If the bonding time is too short, the bonding will be weak or impossible, affecting the sealing of the chip and making it unable to be used normally; if the bonding time is too long, it will lead to excessive bonding, causing the channel structure of the chip to be blocked.

[0063] When using the above-mentioned vitamin electrochemical detection chip based on finger pressure pump to detect vitamins, the following steps are included:

[0064] 1. Tear off the polyethylene (PE) sealing film at the injection port and buffer port, so that the vent remains open before injection;

[0065] 2. Press the finger massage pump to the bottom, and then seal the vent hole with a sealing film;

[0066] 3. Add 10 μL of sample and buffer to the injection port and buffer port respectively;

[0067] 4. Release the finger pump to make the chip in a negative pressure state;

[0068] 5. After the sample and buffer solution are mixed and fill the detection chamber, tear off the sealing film at the vent hole, open the vent hole, release the remaining air pressure in the finger pump, and return the air pressure in the finger pump to the level of atmospheric pressure, so that the pressure in the chip reaches a balanced state, acting as a stop valve to prevent the buffer solution from flowing into the finger pump through the detection chamber;

[0069] 6. Connect the finger interface of the chip to the electrochemical workstation through a customized interface to perform electrochemical detection on the sample.

[0070] Comparative Example 1

[0071] The difference between this comparative example and Example 1 is that in this comparative example, a single-layer soft lithography technology is used to make the mold. Single-layer soft lithography technology cannot produce structures with a height exceeding 300μm. When the aspect ratio of the mold is too large, it is easy to cause the center of the structure to collapse and cannot be used normally.

[0072] Comparative Example 2

[0073] The difference between this comparative example and Example 1 is that in this comparative example, no vent is provided. During use, negative pressure will cause the sample to enter the finger pressure pump and fail to fill the entire detection chamber, which not only causes waste but also affects the detection effect.

[0074] Experimental Example 1: Effects of Electrodes Prepared by Different Methods on Electrochemical Detection of Vc

[0075] Treatment group 1: The electrodes prepared by the method of Example 1 of the present invention were used to detect Vc. The sample processing and detection method was as follows: different concentrations (25, 50, 100, 150, 200, 250 μM) of pure ascorbic acid were prepared and detected in the chip. The electrochemical method used was differential pulse voltammetry. The detection results were as follows: Figure 6 The curves in the figure correspond to the detection results of 25, 50, 100, 150, 200, and 250 μM from bottom to top.

[0076] Treatment group 2: Prepare ascorbic acid solution with the same concentration gradient as treatment group 1. Take 40 μL of each concentration and drop it directly on the electrode surface for detection. The electrochemical method used is the same as above. The detection results are as follows: Figure 7 The curves in the figure correspond to the detection results of 25, 50, 100, 150, 200, and 250 μM from bottom to top.

[0077] The results show that the electrochemical response of the detection results in the chip is higher than that of the detection directly on the electrode surface.

[0078] The above is only an embodiment of the present invention, and the common knowledge such as the specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A vitamin electrochemical detection chip based on a finger pressure pump, characterized by: It includes an electrode slot layer, a microstructure layer and a sealing film layer arranged in sequence, and an electrode is installed in the electrode slot layer; the electrode includes a base layer, a silver lead / silver electrode layer, a carbon lead / main electrode layer and an insulating paint layer arranged in sequence; a detection chamber, a connecting channel and a finger pressure pump are arranged on the microstructure layer, and a vent is provided on the connecting channel; the electrode slot layer is made of polydimethylsiloxane.

2. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 1, characterized in that: The electrode slot layer is provided with electrode slots for installing electrodes.

3. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 2, characterized in that: The microstructure layer is prepared by casting polydimethylsiloxane in a mold made by 3D printing. The microstructure layer is also provided with an injection port, a buffer port and a mixing structure, and the mixing structure is connected to the detection chamber through a connecting channel.

4. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 3, characterized in that: The sealing film layer is made of polyethylene film.

5. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 4, characterized in that: The base layer of the electrode is made of polyethylene terephthalate.

6. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 5, characterized in that: The silver lead / silver electrode layer is arranged on the base layer, and the silver lead / silver electrode layer includes an auxiliary electrode, a reference electrode, a working electrode silver base, a lead silver base, and a finger electrode slot silver base.

7. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 6, characterized in that: The carbon lead / main electrode layer is arranged on the base layer, and the carbon lead / main electrode layer includes a working electrode carbon surface, a finger electrode slot carbon surface, and a lead carbon surface.

8. The vitamin electrochemical detection chip based on a finger pressure pump according to claim 7, characterized in that: The insulating paint layer is printed on the base layer using a screen printing process to print ultraviolet curing insulating ink or heat curing insulating ink.

9. The method for preparing a vitamin electrochemical detection chip based on a finger pressure pump according to any one of claims 1 to 8, characterized in that: The steps include: Step 1: Prepare electrodes, prepare a base layer, and use a screen printing process to print a conductive silver paste on the base layer to form an auxiliary electrode, a reference electrode, a working electrode silver base, a lead silver base, and a finger electrode slot silver base; Conductive carbon paste is then printed on the substrate using a screen printing process to form a working electrode carbon surface, a finger electrode slot carbon surface, and a lead carbon surface. UV-curable insulating ink is then printed on the substrate using a screen printing process to form an insulating paint layer, completing the electrode preparation. Step 2: Prepare the electrode slot. The electrode slot is prepared by polydimethylsiloxane inverting mold. The mold used is made by 3D printing. The 3D printing mold material is light-curing resin. Step 3: preparing a microstructure layer by casting polydimethylsiloxane in a mold made by 3D printing; Step 4: Prepare a sealing film layer made of polyethylene film to close and open the vents; Step 5: Assembling: After inserting the electrode into the electrode slot, the electrode slot layer and the microstructure layer are assembled through a bonding process to obtain a vitamin electrochemical detection chip based on a finger pressure pump.

10. The method for preparing a vitamin electrochemical detection chip based on a finger pressure pump according to claim 9, characterized in that: The mold making method in step 2 and step 3 is: (1) Use CAD or SolidWorks to design the 3D drawing of the mold; (2) Import the 3D image in (1) into the 3D printer through Anycubic Photon Workshop; (3) After printing, clean it with alcohol ultrasonic cleaning; (4) Use ultraviolet light curing; (5) Bake on a hot plate at 120°C for 1-2 hours; (6) Plasma cleaning; (7) Spin-coat a release agent on the treated mold surface to obtain the mold.

Images