An antigen antibody reaction cartridge

Abstract

This invention relates to the field of reaction chamber technology, specifically an antigen-antibody reaction chamber, comprising a chamber body with three mounting openings on the outside for mounting windows one, two, and three, respectively. The cross-section of the chamber body is an outer shell, and the top of the outer shell has a top mechanism with rotating pins at both ends inserted into the two sides of the top of the outer shell. One side of the lower outer wall of the top of the outer shell is connected to an antigen chamber, and the other side is connected to an antibody chamber. The lower parts of the antigen chamber and antibody chamber are connected to the second air bladder of the reaction device via gas inlet tubes. The soft baffle in the inlet initially touches the inlet of the inlet tube, and then the inlet begins to break through the soft baffle, causing the reagent inside the antigen chamber to flow out along the long tube and then into the reaction device. The antigen reagent and the antibody reagent flowing into the reaction device at the same time specifically bind, thereby accelerating the specific binding rate of antigen and antibody inside the reaction device.

Description

Technical Field

[0001] This invention relates to the field of reaction cassette technology, specifically an antigen-antibody reaction cassette. Background Technology

[0002] An antigen-antibody reaction refers to the specific binding reaction between an antigen and its corresponding antibody. This reaction can occur both in vivo and in vitro. The process of an antigen-antibody reaction involves a series of chemical and physical changes, including two stages: specific binding of antigen and antibody and non-specific aggregation, as well as a change from hydrophilic colloid to hydrophobic colloid. In vitro reactions, especially in the laboratory, have historically relied on adding a single antigen reagent kit to an antibody reagent kit for specific binding, particularly by allowing the kit to stand and freely collide between the antigen and antibody, resulting in a slow reaction rate. Inhomogeneous mixing of antigen and antibody reagents within the reaction kit also contributes to a slow rate of specific binding. Summary of the Invention

[0003] To address the problems in the prior art, the present invention provides an antigen-antibody reaction kit.

[0004] The technical solution adopted by the present invention to solve its technical problem is as follows: an antigen-antibody reaction box, including a box body, the box body having three mounting openings on its exterior for mounting a first window, a second window, and a third window, the cross-section of the box body being an outer shell, the top of the outer shell having a box top mechanism, and rotating pins at both ends of the box top mechanism being inserted into the two sides of the top of the outer shell, one side of the outer wall below the top of the outer shell being connected to an antigen box, and the other side being connected to an antibody box, the lower parts of the antigen box and the antibody box being connected to the second air bladder of the reaction device via gas supply pipes.

[0005] Preferably, the box top mechanism includes an operating table, and a frame is provided at the center of the operating table. Protrusions are fixed on the left and right sides of the frame, and the protrusions are embedded in the slots. A buckle is fixed at one end of the left side of the frame, and the buckle engages with a rotating shaft. The other end is sleeved on a rotating head fixed in the middle of the box plug. The two ends of the operating table are set as semi-circular end faces, and connecting buckles are fixed on the semi-circular end faces. The connecting buckles are sleeved with rotating pins, and the rotating pins are inserted into the port at the top of the outer shell.

[0006] Preferably, the rotating shaft includes a pull rod, which is inserted into the end face of the side support column. A slot is opened in the middle of the end face of the side support column, and crossbars are respectively provided at both ends of the slot. A support frame is supported inside the crossbars, and a movable rod is embedded inside the side support column.

[0007] Preferably, the antigen box includes a support wall, a first air bladder is fixed to the right side of the support wall, the bottom of the first air bladder is connected to an air supply tube, and the other end of the air supply tube is connected to a second air bladder of the reaction device. An infusion tube is provided at the bottom of the right side of the support wall and passes through the right end face of the support wall. The antigen box is attached to the bottom upper part of the support wall. A top cover is provided at the top of the antigen box. A side rod is provided on one side of the antigen box, and an infusion port is opened on the bottom right side of the antigen box. The upper and lower parts of the support wall are connected to the side wall of the outer shell, and a flexible tube is connected to the upper and lower ends of the side wall. A spring tube is installed from the opening of the side wall on the flexible tube, and a corrugated tube is fixed to the outer wall at the middle position of the flexible tube.

[0008] Preferably, the infusion port includes a support rod, the upper and lower ends of which are fixed to the bottom right end face of the antigen box, and a row of uprights are provided on the inner side of the support rod. A soft stop bar is connected to the front end of the uprights, and the infusion tube is fixed to one end face on the right side of the support wall. The infusion tube has tube walls at the top and bottom, and a long tube is provided in the middle of the tube wall. The right end of the long tube is fixed to the tube wall at the top and bottom, and the two ends of the long tube are semi-arc-shaped.

[0009] Preferably, the reaction device includes a friction rod placed around the periphery of the reaction device and in contact with the outer wall of the reaction device. The bottom sides of the reaction device are attached to the second airbags, the bottom of the second airbags are connected to and fixed to the vertical rod, and a soft spring is placed in the middle of the bottom of the reaction device above the base, while the base is fixed to the lower interior of the outer shell.

[0010] Beneficial effects of this invention:

[0011] (1) When the antigen box moves toward the first air bladder, the infusion port at the bottom of the antigen box also moves synchronously. As the antigen box squeezes the first air bladder, the infusion port also slowly moves to the right until the inner wall of the support rod of the infusion port opens to form a sleeve wall. Then, the soft baffle in the infusion port touches the opening of the infusion tube. After that, the opening breaks through the soft baffle, causing the reagent inside the antigen box to flow out along the long tube and then flow into the reaction device. The antigen reagent and the antibody reagent that flows into the reaction device at the same time will specifically bind.

[0012] (2) This invention utilizes the side support to press the box stopper downwards, causing the four protrusions in the middle of the box stopper to be embedded into the collar. Then, a handle is provided at the front end of the rotating shaft. The handle is rotated by hand, and at this time, the handle drives the rotating shaft. The bottom of the rotating shaft drives the reaction device to rotate, so that the antigen-antibody reagents inside are fully mixed, which accelerates the rate of antigen-antibody specific binding. While the reaction device is rotating, the outer wall of the reaction device rubs against the friction rod. Since the outer wall of the reaction device is coated with a material that easily generates heat through friction, the outer wall rubs against the friction rod as the reaction device rotates, causing the outer wall to generate heat through friction. This heat enters the reaction device, thereby accelerating the rate of antigen-antibody specific binding inside the reaction device. Attached Figure Description

[0013] The invention is further illustrated below with reference to the accompanying drawings and embodiments.

[0014] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0015] Figure 2 for Figure 1 The sectional view shown;

[0016] Figure 3 for Figure 2 The diagram shows the control panel mechanism.

[0017] Figure 4 for Figure 3 The diagram shows the rotating shaft.

[0018] Figure 5 for Figure 4 The diagram shows the side support pillars;

[0019] Figure 6 for Figure 2 The enlarged view of point A shown;

[0020] Figure 7 for Figure 2 The diagram shows the stopper and reaction apparatus.

[0021] Figure 8 for Figure 2 The enlarged view at point B is shown below;

[0022] In the diagram: 1. Box body; 11. Window 1; 12. Window 2; 13. Window 3; 14. Outer shell; 2. Box top mechanism; 21. Operating table; 211. Frame; 212. Rotating shaft; 2121. Pull rod; 2122. Support frame; 2123. Moving rod; 213. Buckle; 214. Rotating head; 215. Slot; 22. Connecting buckle; 23. Rotating pin; 24. Box plug; 241. Protrusion; 242. Collar; 25. Side support; 251. Crossbar; 252. Groove; 3. Antigen box; 31. Support wall; 32. Spring tube; 33. Side rod; 34. Soft tube; 35. Corrugated tube; 36. No. 1 air bag; 361. Gas delivery tube; 37. Top cover; 38. Infusion port; 381. Support rod; 382. Vertical rod; 383. Soft stop bar; 39. Infusion tube; 391. Tube wall; 392. Tube opening; 393. Long tube; 4. Antibody box; 5. Reaction device; 51. Friction rod; 52. Outer wall; 53. No. 2 air bag; 54. Soft spring; 55. Base; 56. Vertical rod. Detailed Implementation

[0023] To make the technical means, creative features, objectives, and effects of this invention easier to understand, the following detailed description is provided in conjunction with specific implementation methods.

[0024] like Figures 1-8 As shown, an antigen-antibody reaction box of the present invention includes a box body 1. The box body 1 has three mounting ports on its exterior, for mounting a first window 11, a second window 12, and a third window 13, respectively. The box body 1 includes an outer shell 14. The top of the outer shell 14 is provided with a box top mechanism 2, and the rotating pins 23 at both ends of the box top mechanism 2 are inserted into the two sides of the top of the outer shell 14. One side of the outer wall 52 below the top of the outer shell 14 is connected to an antigen box 3, and the other side is connected to an antibody box 4. The lower parts of the antigen box 3 and the antibody box 4 are respectively connected to the second air bladder 53 of the reaction device 5 through an air supply pipe 361.

[0025] Specifically, the box top mechanism 2 includes an operating table 21, and a frame 211 is provided at the center of the operating table 21. Protrusions 241 are fixed on the left and right sides of the frame 211, and the protrusions 241 are embedded in the slots 215. A buckle 213 is fixed at one end of the left side of the frame 211, and the buckle 213 engages with the rotating shaft 212. The other end is sleeved on the rotating head 214 fixed in the middle of the box plug 24. The two ends of the operating table 21 are set as semi-circular end faces, and a connecting buckle 22 is fixed on the semi-circular end face. The connecting buckle 22 is sleeved on the rotating pin 23, and the rotating pin 23 is inserted into the port at the top of the outer shell 14. In practice, at the top of the box body 1, on the box top mechanism 2, firstly, the connecting buckles 22 at both ends of the rotating operating table 21 are rotated. Simultaneously, the rotating pins 23 connected to the connecting buckles 22 rotate together with them, thus removing the rotating pins 23 from the ports at both ends of the top of the outer shell 14. Then, the operating table 21 is opened, and the antigen box 3 and antibody box 4 are removed from the box body 1, respectively filled with antigen and antibody reagent kits, and then placed back into the box body 1. The above steps are then repeated, except that this time... The previous procedure was the opposite. Using the connecting buckle 22, the rotating pin 23 was inserted into the top port of the outer casing 14. After the reagents inside the antigen box 3 and antibody box 4 were incorporated into the reaction apparatus 5, the rotating shaft 212 was removed from the clip 213 fixed on the operating table 21, making the shaft vertical. Then, it was pressed downwards against the stopper 24, causing the protrusions 241 on both sides of the stopper 24 to dislodge from the slots 215, thus detaching the stopper 24 from the stand 211 on the operating table 21. Afterwards, the stopper... The stopper 24 is lowered and then positioned at the opening of the reaction apparatus 5. At this point, the side support 25 presses the stopper 24 downwards, causing the four protrusions 241 in the middle of the stopper 24 to engage with the collar 242. Then, the handle 216 at the front end of the rotating shaft 212 is opened and rotated by hand. This causes the handle 216 to drive the rotating shaft 212, and the bottom of the rotating shaft 212 drives the reaction apparatus 5 to rotate, ensuring thorough mixing of the internal antigen-antibody reagents and accelerating the rate of specific antigen-antibody binding. As the reaction apparatus 5 rotates... Simultaneously, the outer wall 52 of the reaction device 5 rubs against the friction rod 51. Since the outer wall 52 of the reaction device 5 is coated with a material that easily generates heat through friction, the rotation of the reaction device 5 causes the outer wall 52 to rub against the friction rod 51, generating heat through friction. This heat then enters the reaction device 5, thereby accelerating the specific binding rate of antigen and antibody inside the reaction device 5. This step can be observed through window 11 at the front end of the box 1, showing the rotation speed of the reaction device 5.

[0026] Specifically, the rotating shaft 212 includes a pull rod 2121, which is inserted into the end face of the side support 25. A slot 252 is opened in the middle of the end face of the side support 25, and a crossbar 251 is respectively provided at both ends of the slot 252. A support frame 2122 is supported inside the crossbar 251, and a movable rod 2123 is embedded inside the side support 25. In specific operation, when the rotating shaft 212 is removed from the buckle 213 on the frame 211 on the operating table 21, the compression plug 24 is then opened, causing the protrusions 241 around the plug 24 to fall out of the slots 215 on the frame 211. Then the plug 24 is moved downward. At this time, the pull rod 2121 on the side support 25 on the rotating shaft 212 is moved downward along the slot 252 via the side support 25. Every time the pull rod 2121 moves one horizontal bar 251 height along the slot 252, the support frame 2122 needs to be moved downward by one horizontal bar 251 position. During the downward movement of the pull rod 2121, the pull rod 2121 pushes the moving rod 2123 downward. At this time, the moving rod 2123 will drive the protrusions 241 around the plug 24 connected at the lower end to engage with the collar 242 at the top of the reaction device 5.

[0027] Specifically, the antigen box 3 includes a support wall 31. A first air bladder 36 is fixed to the right side of the support wall 31. The bottom of the first air bladder 36 is connected to an air supply tube 361, and the other end of the air supply tube 361 is connected to a second air bladder 53 of the reaction device 5. An infusion tube 39 is provided at the bottom of the right side of the support wall 31 and passes through the right end face of the support wall 31. The antigen box 3 is attached to the bottom of the support wall 31. A top cover 37 is provided at the top of the antigen box 3. A side rod 33 is provided on one side of the antigen box 3. An infusion port 38 is opened on the bottom right side of the antigen box 3. The upper and lower parts of the support wall 31 are connected to the side wall of the outer shell 14. A flexible tube 34 is connected to the upper and lower ends of the side wall. A spring tube 32 is installed from the opening of the flexible tube 34 on the side wall. A corrugated tube 35 is fixed to the outer wall of the middle position of the flexible tube 34. In practice, after placing the antigen and antibody reagents into the corresponding antigen cartridge 3 and antibody cartridge 4, respectively, and then placing them in the corresponding positions on the box body 1, the flexible tube 34 on the side wall of the box body 1 is squeezed. Since the flexible tube 34 has spring tubes 32 at both ends, as the flexible tube 34 extends inward, the spring tubes 32 also lengthen. Then, the flexible tube 34 pushes the corrugated tube 35 connected to the side wall towards the antigen cartridge 3, pushing the antigen cartridge 3 towards the position of the first air bladder 36. Afterward, the antigen cartridge 3 squeezes the first air bladder 36, causing the first air bladder 36 to release its internal gas, which is then transported by the gas delivery tube 361. When the second air bladder 53 is opened, it inflates. As more gas is injected, it pushes the reaction device 5 upward. At this time, the reagent inside the antigen box 3 flows into the reaction device 5 from the infusion port 38 at the bottom of the box through the infusion tube 39. Then, the soft tube 34 is released. Due to gravity, the reaction device 5 squeezes the second air bladder 53, causing the gas in the second air bladder 53 to return to the first air bladder 36. The first air bladder 36, due to its internal inflation, squeezes the antigen box 3, pushing it away from the first air bladder 36. As the antigen box 3 moves to the left, the infusion tube 39 at the bottom of the antigen box 3 closes. Simultaneously, since the antigen box 3 and antibody box 4 are symmetrical, the processes described above for antigen box 3 and antibody box 4 are synchronized.

[0028] Specifically, the infusion port 38 includes a support rod 381, the upper and lower ends of which are fixed to the bottom right end face of the antigen box 3. A row of uprights 382 are arranged on the inner side of the support rod 381. A soft stop bar 383 is connected to the front end of the uprights 382. The infusion tube 39 is fixed to one end face of the right side of the support wall 31. The infusion tube 39 has tube walls 391 on the upper and lower sides. A long tube 393 is provided in the middle of the tube wall 391. The outer side of the right end of the long tube 393 is fixed to the tube wall 391 on the upper and lower sides. The two ends of the long tube 393 are semi-arc-shaped. In actual operation, as the antigen box 3 moves towards the first airbag 36, the infusion port 38 at the bottom of the antigen box 3 also moves synchronously. As the antigen box squeezes the first airbag 36, the infusion port 38 slowly moves to the right until the inner wall of the support rod 381 of the infusion port 38 forms a sleeve wall 391. Then, the soft baffle 383 in the infusion port 38 touches the opening 392 of the infusion tube 39. After that, the opening 392 breaks through the soft baffle 383, causing the reagent inside the antigen box 3 to flow out along the long tube 393 and then into the reaction device 5. The antigen reagent and the antibody reagent flowing into the reaction device 5 at the same time will specifically bind.

[0029] Specifically, the reaction device 5 includes a friction rod 51, which is placed around the periphery of the reaction device 5 and adheres to the outer wall 52 of the reaction device 5. Secondary airbags 53 are attached to both sides of the bottom of the reaction device 5. The bottom of the secondary airbags 53 is connected to and fixed to a vertical rod 56. A soft spring 54 is placed above a base 55 at the center of the bottom of the reaction device 5, and the base 55 is fixed to the lower interior of the outer casing 14. In operation, when the gas inside the primary airbag 36 is compressed, it enters the secondary airbag 53 through the gas delivery pipe 361. The expansion of the secondary airbag 53 then propels the reaction device 5 upwards to receive reagents.

[0030] The working principle of the antigen-antibody reaction kit provided by this invention is as follows:

[0031] The specific machine workflow is as follows: First, the connecting buckles 22 at both ends of the rotating operating table 21 are rotated. Simultaneously, the rotating pins 23 connected to the connecting buckles 22 rotate together with them, thus removing the rotating pins 23 from the ports at both ends of the top of the outer casing 14. Then, the operating table 21 is opened, and the antigen box 3 and antibody box 4 are removed from the box 1. Antigen and antibody reagents are then loaded into the boxes 1 respectively. The rotating buckles 22 are rotated to insert the rotating pins 23 into the ports at the top of the outer casing 14. The antigen and antibody reagents are then placed into their respective antigen boxes 3 and antibody boxes 4, and placed in their corresponding positions on the box 1. At this point, by squeezing the flexible tube 34 on the side wall of the box 1, which is designed with spring tubes 32 at both ends, the flexible tube 34 extends inwards, causing the spring tubes 32 to stretch. Then, the flexible tube 34 pushes the corrugated tube 35 connected to the side wall towards the antigen box 3. The movement propels the antigen box 3 towards the position of the first air bladder 36. Then, the antigen box 3 squeezes the first air bladder 36, causing it to release its internal gas. This gas is then transported to the second air bladder 53 via the gas inlet tube 361, causing it to inflate. As more gas is introduced, the reaction device 5 moves upwards. At this time, the reagent inside the antigen box 3 flows from the inlet port 38 at the bottom of the box through the inlet tube 39 into the reaction device 5. The soft tube 34 is then released. Due to gravity, the reaction device 5 squeezes the second air bladder 53, causing the gas in the second air bladder 53 to return to the first air bladder 36. The first air bladder 36, now inflated, squeezes the antigen box 3 again, pushing it away from the first air bladder 36. As the antigen box 3 moves to the left, the inlet tube 39 at the bottom of the antigen box 3 closes. Meanwhile, since antigen box 3 and antibody box 4 are symmetrical, the antigen box 3 and antibody box 4 described above are processed simultaneously.

[0032] Then, after the reagents inside the antigen box 3 and antibody box 4 are incorporated into the reaction apparatus 5, the rotating shaft 212 is removed from the clip 213 fixed on the operating table 21, making the rotating shaft 212 vertical. Then, it is pressed downwards against the plug 24, causing the protrusions 241 on both sides of the plug 24 to disengage from the slot 215, allowing the plug 24 to detach from the stand 211 on the operating table 21. The plug 24 then falls and stands upright at the opening of the reaction apparatus 5. At this point, the plug 24 is pressed downwards using the side support 25, causing the four protrusions 241 in the middle of the plug 24 to engage with the collar 242. Then, the handle 216 at the front end of the rotating shaft 212 is rotated by hand. Hand 216 drives rotating shaft 212, and the bottom of rotating shaft 212 drives reaction device 5 to rotate, so that the antigen-antibody reagents inside are fully mixed, accelerating the rate of specific binding of antigen and antibody. While the reaction device 5 is rotating, the outer wall 52 of the reaction device 5 rubs against the friction rod 51. Since the outer wall 52 of the reaction device 5 is coated with a material that easily generates heat through friction, the friction between the outer wall 52 and the friction rod 51 caused by the rotation of the reaction device 5 generates heat, which is then introduced into the reaction device 5. This accelerates the rate of specific binding of antigen and antibody inside the reaction device 5. This step can be seen through window 11 at the front end of the box 1, which shows the speed of rotation of the reaction device 5.

[0033] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of protection claimed by the present invention. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antigen-antibody reaction cartridge, characterized by: The utility model provides a kind of antigen-antibody reaction device, including box (1), three installation openings are opened outside the box (1), respectively install No. The box top mechanism (2) includes operation platform (21), and operation platform (21) center is provided with rack (211), the left and right sides of rack (211) are fixed with lug (241), and the lug (241) is embedded in the clamping groove (215) of operation platform (21), the left end of rack (211) is fixed with buckle (213), the buckle (213) is clamped rotating shaft (212), the other end of rotating shaft (212) is sleeved in the rotating head (214) that box plug (24) middle part is fixed, the two ends of operation platform (21) are provided with semicircular end face, and semicircular end face is fixedly connected with buckle (22), the buckle (22) is sleeved with rotating pin (23), and the rotating pin (23) is inserted into the port of the top of shell (14). The box body (1) further includes support wall (31) connected to the sidewall of the shell (14) above and below, the bottom of the support wall is attached to the antigen box (3), one side of the right side of the support wall (31) is fixed with a first air bag (36), the bottom of the first air bag (36) is connected with the gas pipe (361), and the other end of the gas pipe (361) is connected to the second air bag (53) of the reaction device (5), and a liquid delivery pipe (39) is arranged at the bottom of the right side of the support wall (31), and the liquid delivery pipe (39) penetrates the right side end face of the support wall (31), the top of the antigen box (3) is provided with a top cover (37), one side of the antigen box (3) is provided with a side rod (33), and a liquid delivery port (38) is formed in the bottom of the right side of the antigen box (3), the top and bottom of the support wall (31) are connected to the sidewall of the shell (14), and the sidewall is connected with a flexible tube (34) at the top and bottom, and the flexible tube (34) is installed with a spring tube (32) from the beginning of the sidewall, the outer sidewall of the middle position of the flexible tube (34) is fixed with a bellows (35). The reaction device (5) includes a friction rod (51), and the friction rod (51) is placed around the reaction device (5) and attached to the outer wall (52) of the reaction device (5), the bottom of the reaction device (5) is attached to the second air bag (53) on both sides, the bottom of the second air bag (53) is connected and fixed to the vertical rod (56), and the bottom of the reaction device (5) is placed above the soft spring (54) of the base (55), and the base (55) is fixed to the lower interior of the shell (14).

2. An antigen-antibody reaction cartridge according to claim 1, characterized by: The infusion port (38) comprises a support rod (381), the upper and lower ends of which are fixed to the right end face of the bottom of the antigen box (3), and a row of vertical rods (382) are arranged on the inner side of the support rod (381), the front end of the vertical rod (382) is connected with a soft stop rod (383), the infusion tube (39) is fixed to one end face on the right side of the support wall (31), and the upper and lower sides of the infusion tube (39) are provided with tube walls (391), the middle of the tube wall (391) is provided with a long tube (393), the right end outer side of the long tube (393) is fixed to the tube wall (391) in an up-down manner, and the two end tube openings (392) of the long tube (393) are semicircular.

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