Precision Sample Injection Device for Porous Plates That Can Be Tracked

Abstract

The present invention relates to a precision sample injection device for a porous plate capable of tracking, and more particularly to a precision sample injection device for a porous plate capable of tracking as follows, which can prevent cross-contamination that may occur when injecting a large amount of samples into a porous plate by manual operation and diagnostic errors caused by injecting samples into the wrong wells or repeated injection, and can simply record and track the information of the wells into which the samples have been injected. The present invention relates to a precision sample injection device for a porous plate capable of injecting multiple samples into a porous plate and being efficiently used in mixed testing and the like and capable of tracking.

Description

Technical Field

[0001] The present invention relates to a precision sample injection device for a porous plate capable of tracking, and more particularly to a precision sample injection device for a porous plate capable of tracking, which can not only inject a sample into a hole of a porous plate while preventing cross-contamination and diagnostic errors, but also simply record and track information of the hole into which the sample is injected. Background Art

[0002] For molecular diagnosis of a virus, it is possible to determine whether a pathogenic bacterium is infected through a nucleic acid extraction step of extracting nucleic acid from a clinical sample and a real-time polymerase chain reaction (PCR) step of amplifying the extracted nucleic acid for qualitative and quantitative analysis.

[0003] Since the polymerase chain reaction (PCR) was invented by Kary Mullis in 1985, it has been widely used in molecular biology and molecular diagnosis because it can quickly and easily amplify a specific DNA.

[0004] If PCR / RTPCR (polymerase chain reaction / reverse transcription polymerase chain reaction) is used, it is possible to confirm the presence or absence of a specific DNA / RNA in a living sample with high sensitivity, so it is widely used in diagnosing whether a pathogenic microorganism such as a virus is infected. Moreover, since the number of pathogens can be accurately quantified by methods such as qPCR (quantitative PCR), it is beneficial for monitoring the treatment effects of viruses such as HIV, HCV, and HBV.

[0005] Generally, only a highly sensitive molecular diagnostic method can correctly diagnose asymptomatic infected persons and early infected persons, so there is an urgent need for a technology capable of batch molecular diagnostic testing.

[0006] For batch detection, a pooling test that aggregates multiple specimens into one solution for detection has been used in the past. For blood screening tests for detecting high-risk viruses such as HIV, HBV, and HCV, a detection method that combines six samples for detection has been used in the past. In the prior art, such a pooling test has been proposed in the past. However, the process of pooling batch specimens requires correct recording of information of each clinical specimen and prevention of cross-contamination between each pooled test group. Therefore, if it is carried out manually, many mistakes may occur. Although expensive automated liquid dispensing devices for pooling have been commercialized, multiple such devices are required to process a large number of samples simultaneously. Therefore, it is practically limited to quickly process a large number of samples using expensive automated devices that occupy a large area.

[0007] Although fully automated systems for batch testing have been developed, the equipment of such systems is very complex and expensive, and does not provide a pooling function, so it cannot meet the urgently needed global testing requirements.

[0008] In the nucleic acid extraction step, generally 0.2 ml to 1 ml of clinical samples are taken and about 1 ml of extraction reagent solution is added thereto for extraction, so it is necessary to be able to hold a maximum of 2 ml of solution. In order to extract nucleic acids from a large number of samples in a short time, it is necessary to use a multi-well plate that can process as many samples as possible. Therefore, as the most common method that can extract from a large number of samples simultaneously, a deeper 96-well plate that can hold 2 ml of solution per well is adopted. Many companies have developed and released equipment for extracting nucleic acids using a deeper 96-well plate for batch testing. Among them are the King Fisher automated nucleic acid extraction equipment developed by Thermo Fisher, the MagNA Pure 96 equipment released by Roche, and the ExiPrep 96 Lite released by BIONEER Corporation, etc.

[0009] At this time, in order to use a 96-well automated nucleic acid extraction equipment, it is necessary to perform the step of adding samples to the 96-well plate. And molecular diagnosis generally has a high-sensitivity detection limit that can detect less than 100 target nucleic acids in the reaction solution and determines it as positive based on this, so it is very sensitive to false positives caused by contamination. For example, even if only 1 nl of a 10^8 / ml positive sample solution contaminates the surrounding negative wells, it may be determined as a false positive. Therefore, when using a 96-well plate to extract nucleic acids, it is very important to avoid droplet contamination of adjacent wells when injecting samples.

[0010] In addition, when repeatedly performing the operation of injecting 96 samples into each well one by one, there will be a problem that wrong results may be obtained by accidentally injecting samples into irrelevant wells. Especially during pooling, during the operation of counting the number of specimens to be pooled one by one, it is easy to make the mistake of accidentally injecting samples into irrelevant wells. To solve this problem, equipment for automatically injecting samples into a 96-well plate has been developed, but it has problems such as high equipment cost and easy wrong operation when processing samples with cotton swabs and swap specimens packed in sample collection containers.

[0011] [Prior Art Documents]

[0012] (Patent Document 1) Korean Patent Gazette No. 10-0637030 (October 16, 2006)

[0013] (Non-Patent Document 1) Ms. NS Sahajpal, The Journal of Molecular Diagnostics, July 29, 2020. Summary of the Invention

[0014] The present invention is proposed to solve the above-mentioned problems, and its purpose is to provide a precision sample injection device for a porous plate that can be tracked, which can not only prevent the following diagnostic errors, but also simply record and track the information of the wells into which the samples are injected. The diagnostic errors are those that may occur when a large number of samples are manually loaded into a porous plate for nucleic acid extraction for single or mixed detection, that is, the diagnostic errors caused by injecting samples into the wrong wells or injecting samples repeatedly.

[0015] In addition, the purpose of the present invention is to provide a precision sample injection device for a porous plate that can be tracked, which can prevent the following errors that may occur when manually loading samples into a porous plate for nucleic acid extraction, that is, the error of cross-contamination that may be caused by dripping samples into the wells adjacent to the wells to be injected with samples.

[0016] The precision sample injection device for a porous plate that can be tracked according to the present invention includes: a main body 100; a porous plate mounting portion 110, which is formed in a groove shape on the upper part of the main body 100 for inserting and mounting a porous plate 200; a protection panel 120, which is located on the top surface of the main body 100 including the porous plate mounting portion 110 and includes an injection port 121, and the injection port 121 is formed to penetrate in the vertical direction so that samples can be injected into a selected well at a selected position; a protection panel moving portion 130 for moving the protection panel 120 so that the injection port 121 is located on the selected well; a position detection portion for detecting the position information of the well of the porous plate 200 where the injection port 121 is located; a barcode sensor 106 for obtaining the barcode information of the sample; and a circuit board 103 for recording and communicating the position information of the well and the barcode information of the sample, and controlling the protection panel moving portion 130.

[0017] The porous plate mounting portion 110 can be formed to have a height that separates the protection panel 120 and the porous plate 220.

[0018] The injection port 121 can be formed such that the lower diameter is smaller than the upper diameter, and a hollow anti-pollution plate 122 can be provided at the lower part of the injection port 121.

[0019] The protection panel moving part may include a first protection panel moving part, and the first protection panel moving part may include: a Y-axis fixing bracket 131 coupled to a corner of the main body 100; a Y-axis part 132 connecting the Y-axis fixing bracket 131 in the Y-axis direction; a Y-axis slider 133 moving along the Y-axis part 132 in the Y-axis direction; an X-axis part 134 having both ends coupled to the Y-axis slider 133 in the X-axis direction; and an X-axis slider 135 moving along the X-axis part 134 and coupled to the top surface of the protection panel 120.

[0020] The protection panel moving part may include a second protection panel moving part, and the second protection panel moving part may include: a Y-axis fixing bracket 131-1 coupled to a corner of the main body 100; a Y-axis screw 139-1 connecting the Y-axis fixing bracket 131-1 in the Y-axis direction; a Y-axis slider 133-1 moving along the Y-axis screw 139-1 in the Y-axis direction; an X-axis screw 139-2 having both ends coupled to the Y-axis slider 133-1 in the X-axis direction; and an X-axis slider 135-1 moving along the X-axis screw 139-2 and coupled to the top surface of the protection panel 120.

[0021] The second protection panel moving part may further include a motor 139-3 for operating the Y-axis screw 139-1 and the X-axis screw 139-2.

[0022] The position detection part may include: a plurality of permanent magnets 124 provided on the protection panel 120; and a magnetic sensor 105 formed on the main body 100 and for sensing the magnetic force of the permanent magnets 124.

[0023] The position detection part may further include: a pure iron bar 104 formed on the magnetic sensor 105 and formed to have a length corresponding to the moving length of the protection panel 120.

[0024] The position detection part may include: at least one permanent magnet 124 provided on the Y-axis slider 133-1 and the X-axis slider 135-1; and a magnetic sensor 105 formed on the main body 100 and for sensing the magnetic force of the permanent magnets 124.

[0025] The position detection part may include: one or more permanent magnets 124 provided on the Y-axis sliding block 140; and a magnetic sensor 105 for sensing the magnetic force of the permanent magnets 124.

[0026] The protection panel moving part may further include: one or more Y-axis screws 139-1; and a Y-axis driving part 141 connecting the one or more Y-axis screws 139-1 to operate the one or more Y-axis screws 139-1 simultaneously.

[0027] The Y-axis driving unit 141 may include: one or more permanent magnets 124; and a magnetic sensor 105 for sensing the magnetic property of the permanent magnet 124.

[0028] The precision sample injection device for a porous plate capable of tracking according to the present invention can prevent diagnostic errors. Therefore, not only can a large number of tests be performed without the risk of diagnostic errors, but also all sample injection operations performed by an operator can be tracked and managed. Therefore, it has the advantage of being able to improve the diagnostic accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figures 1 to 3 FIG. is a perspective view for representing the precision sample injection device for a porous plate capable of tracking according to the present invention.

[0030] Figure 4 FIG. is a perspective view for representing a pure iron rod, a sensor, etc. installed inside the main body of the precision sample injection device for a porous plate capable of tracking according to the present invention.

[0031] Figure 5 FIG. is a schematic diagram for representing the precision sample injection device for a porous plate capable of tracking according to the present invention.

[0032] Figures 6 to 11 FIG. is another perspective view for representing the precision sample injection device for a porous plate capable of tracking according to the present invention.

[0033] Figures 12 to 13 FIG. is a partial perspective view for representing the precision sample injection device for a porous plate capable of tracking according to the present invention.

[0034] Figures 14 to 15 FIG. is another partial perspective view for representing the precision sample injection device for a porous plate capable of tracking according to the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0035] Hereinafter, a precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

[0036] A precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention includes a main body 100, a porous plate mounting portion 110, a protection panel 120, a protection panel moving portion 130, a position detection portion, a barcode sensor 106, and a circuit board 103.

[0037] The main body 100 may be composed of a main body upper plate 101 and a main body lower plate 102 that are divided in the vertical direction and can be combined.

[0038] The porous plate mounting portion 110 is a structure for inserting and disposing a porous plate 200 on the main body 100, and is formed in a groove shape corresponding to the shape of the porous plate 200 on the main body 100 to place and dispose the porous plate 200.

[0039] The protection panel 120 is located on the top surface of the main body 100 including the porous plate mounting portion 110. The protection panel 120 includes an injection port 121 which is formed to penetrate in the vertical direction so that a sample can be injected at a selected position. At this time, the injection port 121 is preferably formed at the center of the protection panel 120, but is not limited thereto. Moreover, the protection panel 120 is formed to be movable along the top surface of the main body 100, so that the injection port 121 for injecting a sample can be located above a selected hole, and the remaining holes can be covered by the protection panel 120, thereby preventing cross-contamination during the sample injection process.

[0040] The protection panel moving part 130 is formed to be combined with the protection panel 120 and enable the protection panel 120 to move horizontally. Thereby, the protection panel 120 is moved horizontally so that the injection port 121 is located above the required hole.

[0041] The position detection part is composed of a magnetic sensor 105, and can obtain the position information of the holes. The barcode sensor 106 can obtain the barcode information of the specimen sample. The circuit board 103 is used to identify the position information of the holes and the barcode information of the specimen sample, and record them in a storage device such as a computer. For this purpose, the circuit board 103 includes a communication device.

[0042] That is, in the precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention, the position of the injection port 121 based on the position information of the holes is input in real time through the position detection part, and the barcode information of the specimen sample is input in real time through the barcode sensor 106. Thus, mistakes can be prevented through the input records, and the generated mistakes can be tracked.

[0043] Thereby, during the process of an operator repeatedly injecting a large number of specimen samples, the occurrence of diagnostic mistakes such as the operator repeatedly loading specimen samples into other holes can be prevented.

[0044] As described above, the precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention has the following effects: that is, by inputting and recording the position information of the holes and the barcode information of the sample in real time, the wrong operations of the operator can be prevented, and the generated mistakes can be tracked.

[0045] A more detailed description will be given of the precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention.

[0046] As described above, the protection panel 120 is located above the multi-well plate 200 and moves in the horizontal direction, and the protection panel 120 is disposed upwardly away from the multi-well plate 200. That is, when the protection panel 120 moves in the horizontal direction so that the injection port 121 is located at the selected well, the sample on the multi-well plate 200 may contact the protection panel 120 and be contaminated. This may cause the sample to contaminate other wells.

[0047] Therefore, the perforated plate mounting part 110 has a height that allows the perforated plate 200 to be spaced apart from the protective panel 120 when the perforated plate 200 is mounted. That is, the perforated plate mounting part 110 is formed to space the protective panel 120 and the perforated plate 200 apart, thereby preventing the sample from contacting the bottom surface of the protective panel 120.

[0048] At this time, the porous plate mounting portion 110 is preferably formed so that the protective panel 120 and the porous plate 200 are separated by a gap of 1mm to 5mm, but as long as the sample can be smoothly injected into the selected hole and contact with the sample can be prevented, various embodiments can of course be implemented without restriction.

[0049] Also, the protection panel 120 may further include a panel handle 123 formed on a top surface of the protection panel 120 , and an operator may move the protection panel 120 through the panel handle 123 .

[0050] The injection port 121 is formed so that the lower diameter is smaller than the upper diameter, and a hollow anti-pollution plate 122 is provided at the lower portion of the injection port 121. The anti-pollution plate 122 is formed of a material capable of absorbing a sample.

[0051] That is, when a sample is injected into a hole of the multi-well plate 200 by a pipette, the sample on the outside of the pipette may be stained into the groove of the injection port 121 and contaminate other holes, thereby causing cross contamination. Therefore, an anti-contamination plate 122 is provided at the lower part of the injection port 121, and the anti-contamination plate 122 can be replaced to prevent cross contamination when contaminated.

[0052] At this time, the hollow of the anti-pollution plate 122 is preferably formed to be smaller than the diameter of the hole. Thus, the sample can be prevented from entering other holes. More preferably, the diameter of the injection port 121 is formed to be larger than the diameter of the hole so that the sample can be injected, and the hollow of the anti-pollution plate 122 is formed to be smaller than the diameter of the hole so that the anti-pollution plate 122 can be replaced, thereby preventing the sample from entering other holes as described above. At this time, the anti-pollution plate 122 can also be formed into a polygonal shape such as a quadrilateral or pentagon, but is preferably formed into a hollow donut shape.

[0053] Reference Figures 1 to 4, the protection panel moving part 130 may include a first protection panel moving part, and the first protection panel moving part includes: a Y-axis fixing bracket 131 coupled to a corner of the main body 100; a Y-axis part 132 connecting the Y-axis fixing bracket 131 in the Y-axis direction; a Y-axis slider 133 moving along the Y-axis part 132 in the Y-axis direction; an X-axis part 134 having both ends coupled to the Y-axis slider 133 in the X-axis direction; and an X-axis slider 135 moving along the X-axis part 134 and coupled to the protection panel 120.

[0054] That is, the first protection panel moving part can move the X-axis slider 135 along the X-axis part 134 to control the position of the injection port 121 in the X-axis direction, and move the Y-axis slider 133 along the Y-axis part 132 to control the position of the injection port 121 in the Y-axis direction.

[0055] Moreover, in order to be able to stop at a fixed position of each hole in the perforated plate 200, shaft grooves 137 configured as grooves are formed at regular intervals on the Y-axis part 132 and the X-axis part 134, and stopping at the fixed position can be easily achieved through a ball plunger 138. With this structure, it is possible to easily align the positions of all the holes in the perforated plate 200 and the injection port 121 of the protection panel 120 by manual operation.

[0056] Moreover, referring to Figures 6 to 15 , the protection panel moving part may further include an automated second protection panel moving part.

[0057] The second protection panel moving part includes: a Y-axis fixing bracket 131-1 coupled to a corner of the main body 100; a Y-axis screw 139-1 connecting the Y-axis fixing bracket 131-1 in the Y-axis direction; a Y-axis slider 133-1 moving along the Y-axis screw 139-1 in the Y-axis direction; an X-axis screw 139-2 having both ends coupled to the Y-axis slider 133-1 in the X-axis direction; an X-axis slider 135-1 moving along the X-axis screw 139-2 and coupled to the top surface of the protection panel 120; and a motor 139-3 for supplying power to operate the Y-axis screw 139-1 and the X-axis screw 139-2.

[0058] The Y-axis screw 139-1 meshes with the Y-axis slider 133-1, and both ends of the Y-axis screw 139-1 are coupled to the Y-axis fixing bracket 131-1, so that the Y-axis screw 139-1 can rotate axially in the Y-axis direction, and the Y-axis screw 139-1 is configured such that the Y-axis slider 133-1 can move in the Y-axis direction through axial rotation.

[0059] At least one Y-axis screw 139-1 may be provided, and one or more Y-axis screws 139-1 are configured to be operable simultaneously by a Y-axis driving part 141. The Y-axis driving part 141 may be composed of a timing belt.

[0060] The X-axis screw 139-2 meshes with the X-axis slider 135-1, and both ends of the X-axis screw 139-2 are coupled to the Y-axis slider 133-1, so that the X-axis screw 139-2 can perform axial rotation in the X-axis direction, and the X-axis screw 139-2 is configured such that the X-axis slider 135-1 can move in the X-axis direction through the axial rotation.

[0061] The electric motor 139-3 can be located at the lower part of the main body or above the Y-axis slider 133-1, and can be connected to the Y-axis screw 139-1 and the X-axis screw 139-2 through connecting members such as gears or synchronous belts.

[0062] As described above, when moving the Y-axis slider 133-1 and the X-axis slider 135-1 by the electric motor 139-3 for automation, it has the advantage of being able to perform more precise position control compared to the operation actions of the operator.

[0063] The position detection unit includes a plurality of permanent magnets 124 provided on the protection panel 120 and magnetic sensors 105 for sensing the magnetism of the permanent magnets 124.

[0064] In order to obtain accurate position information, the permanent magnets 124 are preferably arranged in a plurality along the cross direction formed by the X-axis direction and the Y-axis direction from the center of the protection panel 120. The magnetic sensors 105 can be provided between the main body upper plate 101 and the main body lower plate 102 that form the main body 100 in the up-down direction. In particular, the magnetic sensors 105 are preferably provided above the circuit board 103 between the main body upper plate 101 and the main body lower plate 102.

[0065] Moreover, the magnetic sensors 105 are preferably provided on the circuit board 103 and are arranged corresponding to the rows and columns of the holes forming the porous plate 200.

[0066] As described above, the position detection unit can detect the position information of the injection port 121 and the holes under the movement of the protection panel 120 by the magnetic sensors 105 identifying the permanent magnets 124 provided on the protection panel 120.

[0067] Moreover, the position detection unit may further include a pure iron rod 104, the pure iron rod 104 is configured such that the magnetic sensors 105 are located at the center of the length direction of the pure iron rod 104, and the pure iron rod 104 is formed to have a predetermined length in the rows and columns of the holes.

[0068] That is, since the protection panel 120 can move to all the holes, in order to detect its position, it is necessary to include corresponding magnetic sensors 105 for each hole. For example, in the case of a 96-well plate, 96 magnetic sensors 105 are required, which is not conducive to cost and space utilization.

[0069] Thus, the position information sensor of the present invention can save the setting of the magnetic sensor 105 by including a pure iron rod 104. The pure iron rod 104 can be easily magnetized by a magnet. Thus, when the permanent magnet 124 is located above the pure iron rod 104, the positions in the X-axis direction and the Y-axis direction can be detected at any position.

[0070] Moreover, since the pure iron rod 104 can detect the position through magnetization caused by the movement of the protection panel 120, the pure iron rod 104 is preferably formed to have a length corresponding to the movement distance of the protection panel 120.

[0071] Moreover, when the protection panel moving part is composed of an automated second protection panel moving part, the rotation speed of each axis screw can be counted by the motor 139-3, and thereby the moving positions of the Y-axis slider 133-1 and the X-axis slider 135-1 can be grasped, and further the position of the injection port can be grasped. Therefore, the number of the above-mentioned magnet sensors can also be reduced to one or two.

[0072] The position detection part includes one or more permanent magnets 124 arranged in the Y-axis slider 140 and the Y-axis driving part 141, and a magnetic sensor 105 for sensing the permanent magnet 124.

[0073] In order to grasp accurate position information, the permanent magnets 124 are preferably arranged inside a gear or a synchronous pulley, but are not limited thereto. The magnetic sensor 105 can be arranged adjacent to the gear or the synchronous pulley.

[0074] The Y-axis slider 133-1 and the X-axis slider 135-1 can be further provided with permanent magnets 124, and the main body upper plate 101 can be further provided with a magnetic sensor 105 for sensing the permanent magnet 124.

[0075] As described above, the position detection part can detect the position information of the injection port 121 and the hole under the movement of the protection panel 120 by the magnetic sensor 105 identifying the permanent magnets 124 arranged in the Y-axis slider 140, the Y-axis driving part 141, the Y-axis slider 133-1 and the X-axis slider 135-1. In addition, it can be set that the protection panel 120 moves to the same position when the power is applied.

[0076] In a precision sample injection device for a porous plate capable of tracking according to an embodiment of the present invention having the above structure, a barcode sensor 106 is mounted on a circuit board 103 on which magnetic sensors 105 are arranged and attached, and is tightly assembled through the main body lower plate 102, and only the leads and power terminals of the barcode sensor 106 are exposed. The circuit board has a circuit for controlling the X-axis motor and the Y-axis motor and a circuit for performing wireless communication.

[0077] Accordingly, in the circuit board 103, when the perforated plate 200 is inserted into the perforated plate mounting portion 110 and the protection panel 120 is covered, the plurality of magnetic sensors 105 identify the current position. Conversely, when the protection panel 120 is lifted and flipped backward, no signal input from the magnetic sensors 105 enters the circuit board 103.

[0078] Moreover, the position information of the magnetic sensors 105 in the X-axis direction and the Y-axis direction can be frequently extracted at regular time intervals, thereby recording and tracking the movement history of the protection panel 120. In addition, the barcode sensor 106 can continuously read the sample information attached to the sample container.

[0079] If the position information of the injection port 121 and the barcode information recorded on the time axis are compared in this way, all the information injected by the experimenter into the perforated plate 200 can be easily input, and all the injection processes of the experimenter can be tracked.

[0080] This kind of information can be recorded on the circuit board 103 of the main body and transmitted and recorded to an external computer by wired or wireless means. Thus, the second object of the present invention can be achieved, that is, the object of being able to easily inject sample information and track all processes of the operator during all injection processes can be achieved.

[0081] The above method includes setting the X-axis direction - Y-axis direction movement as the following transfer method: that is, using a linear motor, a servo motor or a stepping motor that can control the position, and using a screw or a timing belt to perform automatic transfer at hole intervals in the X-axis direction and the Y-axis direction. As an example of such an automatic movement method, as Figure 6 shown, the X-axis can be set as a screw, the screw and the motor are connected by gears and transferred at a specified interval in the X-axis direction, and a plurality of screws are arranged on the Y-axis, and the plurality of screws are arranged to be simultaneously operated by the motor and the timing belt, so as to automatically perform movements in the X-axis and Y-axis directions.

[0082] The precision sample injection device for a perforated plate according to an embodiment of the present invention can be controlled by a tablet PC or the like. At this time, the precision sample injection device for a perforated plate according to an embodiment of the present invention can be linked by wireless communication means such as Bluetooth, and can include a socket capable of connecting a cable or the like.

[0083] Moreover, when the precision sample injection device for a perforated plate capable of tracking according to an embodiment of the present invention is configured in an automated manner, it can include a pooling function to be suitable for mixed inspection by an external computer such as the above-mentioned tablet PC. At this time, the mixed inspection can be set by the external computer for the number of samples to be mixed and controlled.

[0084] As an example, when the number of pooled test samples is specified as five, after the barcode sensor recognizes the barcode of the first sample, the protection panel including the injection port does not move and remains stopped on the selected first well W1 until the barcode sensor recognizes the barcode of the fifth sample. Then, when the barcode sensor recognizes the barcode of the sixth sample, the protection panel moves and the injection port is positioned at the selected second well W2. Thus, the position of the injection port is fixed at the second well W2 until the barcode sensor recognizes the barcode of the tenth sample. When the barcode sensor recognizes the barcode of the eleventh sample, the protection panel moves to the position of the selected third well W3.

[0085] The method of injecting a test sample using the precision sample injection device for a multi-well plate capable of tracking according to an embodiment of the present invention will be described below.

[0086] 1. Place the multi-well plate and inject the cell lysis solution

[0087] Open the protection panel 120, insert and set the multi-well plate 200 in the empty multi-well plate mounting portion 110. At this time, it is necessary to pay attention to avoid reversing the direction of the multi-well plate 200. It can be inserted only when the chamfered part of the corner of the multi-well plate 200 matches the chamfered part of the corner of the mounting portion. After installing the multi-well plate 200, repeatedly dispense 200 ul of cell lysis solution 12 times using an eight-channel pipette.

[0088] 2. Cover the protection panel and install the anti-pollution plate

[0089] Cover the protection panel 120 and place the anti-pollution plate 122 on the injection port 121.

[0090] 3. Inject the sample

[0091] Using the panel handle 123, move the injection port to the A1 position, then put the tip on the pipette, make it recognize the barcode attached to the sample tube, open the sample tube, measure a specified amount (e.g., 400 ul) of sample solution with the pipette. At this time, avoid getting a large amount of sample solution on the surface of the tip. After that, pay attention to avoid contact between the sample solution and the anti-pollution plate in the injection port groove while injecting. Then, throw the tip into the trash can, close the lid of the sample tube, move back to the original position and put on a new tip. Repeat the above operations to inject 96 samples into the multi-well plate. Leave it at 60 °C for 10 minutes. After the samples are dissolved, lift the protection panel backward with the panel handle, inject the magnetic bead solution 12 times using an eight-channel pipette, then take out the multi-well plate, install it together with the extraction kit into an automatic nucleic acid extractor, and extract nucleic acid.

[0092] Description of reference numerals

[0093] 100: Main body

[0094] 101: Main body upper plate

[0095] 102: Main body lower plate

[0096] 103: Circuit board

[0097] 104: Pure iron rod

[0098] 105: Magnetic sensor

[0099] 106: Barcode sensor

[0100] 110: Porous plate mounting part

[0101] 120: Protection panel

[0102] 121: Injection port

[0103] 122: Anti-pollution plate

[0104] 123: Panel handle

[0105] 124: Permanent magnet

[0106] 130: Protection panel moving part

[0107] 131: Y-axis fixing bracket

[0108] 132: Y-axis part

[0109] 133: Y-axis slider

[0110] 134: X-axis part

[0111] 135: X-axis slider

[0112] 137: Axis groove

[0113] 138: Ball head plunger

[0114] 131-1: Y-axis fixing bracket

[0115] 133-1: Y-axis slider

[0116] 135-1: X-axis slider

[0117] 139-1: Y-axis screw

[0118] 139-2: X-axis screw

[0119] 139-3: Motor

[0120] 140: Y-axis sliding block

[0121] 141: Y-axis driving part

[0122] 142: Power supply

Claims

1. A precision sample injection device for a porous plate capable of tracking, comprising: A main body; A porous plate mounting portion, formed in a groove shape on the upper part of the main body for inserting and mounting a porous plate; A protection panel, located on the top surface of the main body including the porous plate mounting portion, and including an injection port, the injection port being formed to penetrate in the vertical direction so as to be able to inject a sample into a selected one of the holes at a selected position; A protection panel moving portion for moving the protection panel so that the injection port is located above the selected hole; A position detection portion for detecting the position information of the hole of the porous plate where the injection port is located; A barcode sensor for obtaining the barcode information of the sample; And A circuit board for recording and communicating the position information of the hole and the barcode information of the sample, and controlling the protection panel moving portion.

2. The precision sample injection device for a porous plate capable of tracking according to claim 1, wherein The porous plate mounting portion is formed to have a height that separates the protection panel and the porous plate.

3. The precision sample injection device for a porous plate capable of tracking according to claim 1, wherein The injection port is formed such that the lower diameter is smaller than the upper diameter, A hollow anti-pollution plate is provided at the lower part of the injection port.

4. The precision sample injection device for a porous plate capable of tracking according to claim 1, wherein The protection panel moving portion includes a first protection panel moving portion, The first protection panel moving portion includes: A Y-axis fixing frame combined with the corner of the main body; A Y-axis portion connecting the Y-axis fixing frame in the Y-axis direction; A Y-axis sliding member moving along the Y-axis portion in the Y-axis direction; An X-axis portion with both ends combined with the Y-axis sliding member in the X-axis direction; and An X-axis sliding member moving along the X-axis portion and combined with the top surface of the protection panel.

5. The precision sample injection device for a porous plate capable of tracking according to claim 1, wherein The protection panel moving portion includes a second protection panel moving portion, The second protection panel moving portion includes: A Y-axis fixing frame combined with the corner of the main body; A Y-axis screw connected to the Y-axis fixing frame in the Y-axis direction; A Y-axis sliding member moving along the Y-axis screw in the Y-axis direction; An X-axis screw with both ends combined with the Y-axis sliding member in the X-axis direction; and An X-axis sliding member moving along the X-axis screw and combined with the top surface of the protection panel.

6. The precision sample injection device for a porous plate capable of tracking according to claim 5, wherein The second protection panel moving portion further includes a motor for operating the Y-axis screw and the X-axis screw.

7. The precision sample injection device for a porous plate capable of tracking according to claim 1, wherein The position detection portion includes: a plurality of permanent magnets provided on the protection panel; and a magnetic sensor formed on the main body for sensing the permanent magnets.

8. The precision sample injection device for a porous plate capable of tracking according to claim 7, wherein The position detection unit further includes: a pure iron rod formed on the magnetic sensor and having a length corresponding to the moving length of the protection panel.

9. The precision sample injection device for a porous plate capable of tracking according to claim 5, wherein the position detection unit includes: at least one permanent magnet provided on the Y-axis slider and the X-axis slider; and a magnetic sensor formed on the main body for sensing the permanent magnet.

10. The precision sample injection device for a porous plate capable of tracking according to claim 9, wherein the position detection unit includes: more than one permanent magnet provided on the Y-axis sliding block; and a magnetic sensor for sensing the permanent magnet.

11. The precision sample injection device for a porous plate capable of tracking according to claim 5, wherein the protection panel moving unit further includes: more than one Y-axis screw; and a Y-axis driving unit connecting the more than one Y-axis screw to make the more than one Y-axis screw operate simultaneously.

12. The precision sample injection device for a porous plate capable of tracking according to claim 11, wherein the Y-axis driving unit includes: more than one permanent magnet; and a magnetic sensor for sensing the permanent magnet.

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