Microscope objective lens with adjustable glass cover thickness and image capturing device

Abstract

The present invention discloses a microscope objective lens with adjustable glass cover thickness and an image capturing device, wherein the microscope objective lens with adjustable glass cover thickness comprises: a first lens group with positive refractive power, a second lens group with positive refractive power and a third lens group with negative refractive power, which are coaxially arranged in sequence from the object direction to the image side; the microscope objective lens with adjustable glass cover thickness meets the following conditions: 0.16<|f2 / f1|<2.15; 0.10<|f3 / f1|<2.16; 0.20<|f3 / f2|<2.19; wherein f1 represents the focal length of the first lens group; f2 represents the focal length of the second lens group; and f3 represents the focal length of the third lens group. The present invention has the characteristics of a large adjustable range of the glass cover, a large numerical aperture, a long working distance, high resolution performance and small chromatic aberration.

Description

Technical Field

[0001] The present invention relates to the field of optical technology, and in particular to a microscope objective lens with adjustable glass cover thickness and a device using the objective lens, such as a microscope equipped with the objective lens optical system and an image capturing device equipped with the objective lens optical system. Background Art

[0002] With the rapid development of the biological industry and the electronics industry in recent years, there are more and more demands for observing organisms in culture dishes and observing liquid crystal panels under glass covers. Generally speaking, due to the different thicknesses of the glass covers, the image quality under the same objective lens will change. In order to avoid switching objectives with different glass cover thicknesses too frequently, it is proposed to change the interval on the lens within an objective lens to cope with the deterioration of image quality caused by the change in glass cover thickness. This means that the lens imaging design must take into account the design of different glass cover thicknesses. For such an objective optical system, it is difficult to compensate for axial aberrations, magnification chromatic aberrations, and field curvatures. It is even more difficult to design a long working distance and a larger adjustable range of glass cover thickness.

[0003] Japanese patent document "JP 5277324" discloses a 20X microscope objective lens with an adjustable glass cover from 0mm to 2mm and a working distance of 7.7mm, wherein the adjustable range of the glass cover is not large enough, the numerical aperture is not large enough, and the working distance is not long enough. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention provides a microscope objective lens with adjustable glass cover thickness and an image capturing device to solve the problems of insufficient adjustable range of the glass cover, small numerical aperture, short working distance, large chromatic aberration and low resolution.

[0005] The present invention adopts the following technical solution:

[0006] On the one hand, a microscope objective lens with adjustable glass cover thickness comprises: a first lens group with positive refractive power, a second lens group with positive refractive power and a third lens group with negative refractive power, which are coaxially arranged in sequence from the object direction to the image side. This combination enables the microscope objective lens with adjustable glass cover thickness to have strong positive refractive power, thereby ensuring that the objective lens system OB has a larger numerical aperture and high optical performance.

[0007] In order for the objective optical system to have a larger numerical aperture, stronger resolution and longer working distance, the following conditions must be met:

[0008] 0.16<|f2 / f1|<2.15;

[0009] 0.10<|f3 / f1|<2.16;

[0010] 0.20<|f3 / f2|<2.19;

[0011] Wherein, f1 represents the focal length of the first lens group; f2 represents the focal length of the second lens group; and f3 represents the focal length of the third lens group.

[0012] Preferably, the first lens group is closest to the object side, and includes a first lens and a second lens, the first lens has positive refractive power, and the second lens has positive refractive power.

[0013] Preferably, the second lens group includes one or more cemented lenses, specifically, a third lens and a fourth lens are arranged in sequence from the object direction to the image direction; the third lens has a negative refractive power, and the fourth lens has a positive refractive power; the second lens group is a focusing group, and the change in the thickness of the glass cover plate is adapted by changing the optical interval between the second lens group and the first lens group and the third lens group.

[0014] Preferably, the third lens group includes two or more cemented lenses, specifically, a fifth lens, a sixth lens, a seventh lens, an eighth lens, a ninth lens and a tenth lens are arranged in sequence from the object direction to the image side; the fifth lens has positive refractive power, the sixth lens has negative refractive power, the seventh lens has negative refractive power, the eighth lens has negative refractive power, the ninth lens has negative refractive power, and the tenth lens has negative refractive power.

[0015] Preferably, the first lens group, the second lens group and the third lens group need to meet the following conditions to better control distortion and field curvature:

[0016] 0.6 <f11 / f1<3.0;

[0017] 1.2 <f12 / f1<3.6;

[0018] -1.7 <f21 / f2<0.7;

[0019] -0.9 <f22 / f2<1.5;

[0020] -1.6 <f31 / f3<0.8;

[0021] -0.6 <f32 / f3<1.8;

[0022] -1.6 <f33 / f3<0.8;

[0023] -1.0 <f34 / f3<1.4;

[0024] -1.5 <f35 / f3<0.9;

[0025] -1.0 <f36 / f3<1.4;

[0026] Among them, f11 is the focal length of the first lens, f12 is the focal length of the second lens, f21 is the focal length of the third lens, f22 is the focal length of the fourth lens, f31 is the focal length of the fifth lens, f32 is the focal length of the sixth lens, f33 is the focal length of the seventh lens, f34 is the focal length of the eighth lens, f35 is the focal length of the ninth lens, and f36 is the focal length of the tenth lens.

[0027] Preferably, in order to reduce the on-axis coma and on-axis convergence of a microscope objective lens with adjustable glass cover thickness, the refractive indices of the first lens group, the second lens group and the third lens group are set as follows to further correct the field curvature and chromatic aberration of the objective optical system.

[0028] Specifically, the refractive indexes of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens, and the tenth lens satisfy the following conditions:

[0029] N11≤1.95;

[0030] N12≥1.40;

[0031] N21≥1.60;

[0032] N22≥1.40;

[0033] N31≥1.40;

[0034] N32≥1.50;

[0035] N33≥1.40;

[0036] N34≤2.00;

[0037] N35≤2.01;

[0038] N36≥1.40;

[0039] Among them, N11 is the refractive index of the first lens, N12 is the refractive index of the second lens, N21 is the refractive index of the third lens, N22 is the refractive index of the fourth lens, N31 is the refractive index of the fifth lens, N32 is the refractive index of the sixth lens, N33 is the refractive index of the seventh lens, N34 is the refractive index of the eighth lens, N35 is the refractive index of the ninth lens, and N36 is the refractive index of the tenth lens.

[0040] Preferably, on the basis of the above, the Abbe numbers of the first lens group, the second lens group and the third lens group may be set as follows to reduce the chromatic aberration of the objective optical system.

[0041] The Abbe numbers of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens, and the tenth lens satisfy the following conditions:

[0042] V11≥30;

[0043] V12≤95;

[0044] V21≥35;

[0045] V22≤95;

[0046] V31≤95;

[0047] V32≥20;

[0048] V33≤95;

[0049] V34≥15;

[0050] V35≥15;

[0051] V36≤95;

[0052] Among them, V11 is the Abbe number of the first lens, V12 is the Abbe number of the second lens, V21 is the Abbe number of the third lens, V22 is the Abbe number of the fourth lens, V31 is the Abbe number of the fifth lens, V32 is the Abbe number of the sixth lens, V33 is the Abbe number of the seventh lens, V34 is the Abbe number of the eighth lens, V35 is the Abbe number of the ninth lens, and V36 is the Abbe number of the tenth lens.

[0053] Preferably, in order to reduce on-axis spherical aberration and off-axis coma aberration, the thicknesses of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens and the tenth lens meet the following conditions:

[0054] 3.0 <T31 / T32<3.0;

[0055] 0.1 <T21 / T22<0.6;

[0056] 3.0 <T31 / T32<8.0;

[0057] 1.0 <T33 / T34<5.0;

[0058] 1.5 <T35 / T36<4.0;

[0059] Wherein, T11 is the thickness of the first lens on the optical axis, T12 is the thickness of the second lens on the optical axis, T21 is the thickness of the third lens on the optical axis, T22 is the thickness of the fourth lens on the optical axis, T31 is the thickness of the fifth lens on the optical axis, T32 is the thickness of the sixth lens on the optical axis, T33 is the thickness of the seventh lens on the optical axis, T34 is the thickness of the eighth lens on the optical axis, T35 is the thickness of the ninth lens on the optical axis, and T36 is the thickness of the tenth lens on the optical axis.

[0060] Preferably, the third lens is glued with its surface facing the image side to the surface of the fourth lens facing the object side; the fifth lens is glued with its surface facing the image side to the surface of the sixth lens facing the object side; the seventh lens is glued with its surface facing the image side to the surface of the eighth lens facing the object side; and the ninth lens is glued with its surface facing the image side to the surface of the tenth lens facing the object side.

[0061] On the other hand, an image capturing device includes the microscope objective lens with adjustable glass cover thickness.

[0062] Compared with the prior art, the present invention has the following beneficial effects:

[0063] (1) The present invention arranges the first lens group, the second lens group, and the third lens group in the microscope objective lens with adjustable glass cover thickness so that the first lens group has positive refractive power, the second lens group has positive refractive power, and the third lens group has negative refractive power, thereby making the objective lens optical system have good optical performance;

[0064] (2) The present invention limits the focal length, refractive index, Abbe number and thickness of the first lens group, the second lens group and the third lens group, so that the field curvature, distortion and aberration sensitivity of the microscope objective lens with adjustable glass cover thickness are further improved, thereby ensuring the optical performance of the microscope objective lens with adjustable glass cover thickness, so that the objective lens optical system has the characteristics of a large adjustable range of the glass cover plate, a large numerical aperture, a long working distance, a high resolution performance and a small chromatic aberration.

[0065] Based on the following detailed description of specific embodiments of the present invention in conjunction with the accompanying drawings, those skilled in the art will become more aware of the above and other objects, advantages and features of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] Figure 1 This is a lens composition diagram of a microscope objective optical system with adjustable glass cover thickness according to the first embodiment of the present invention;

[0067] Figure 2 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the first embodiment of the present invention;

[0068] Figure 3This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the first embodiment of the present invention;

[0069] Figure 4 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the first embodiment of the present invention;

[0070] Figure 5 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the first embodiment of the present invention;

[0071] Figure 6 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the first embodiment of the present invention;

[0072] Figure 7 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the first embodiment of the present invention;

[0073] Figure 8 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the first embodiment of the present invention;

[0074] Fig. 9 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the first embodiment of the present invention;

[0075] Fig.10 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the first embodiment of the present invention;

[0076] Fig.11 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the first embodiment of the present invention;

[0077] Fig.12 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the first embodiment of the present invention;

[0078] Fig.13 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the first embodiment of the present invention;

[0079] Fig.14 This is a lens composition diagram of a microscope objective optical system with adjustable glass cover thickness according to the second embodiment of the present invention;

[0080] Fig.15 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the second embodiment of the present invention;

[0081] Fig.16 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the second embodiment of the present invention;

[0082] Fig.17 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the second embodiment of the present invention;

[0083] Fig.18 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the second embodiment of the present invention;

[0084] Fig.19 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the second embodiment of the present invention;

[0085] Fig. 20 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the second embodiment of the present invention;

[0086] Fig.21 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the second embodiment of the present invention;

[0087] Fig. 22 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the second embodiment of the present invention;

[0088] Fig.23 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the second embodiment of the present invention;

[0089] Fig.24 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the second embodiment of the present invention;

[0090] Fig.25 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the second embodiment of the present invention;

[0091] Fig.26 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the second embodiment of the present invention;

[0092] Fig. 27 This is a lens composition diagram of a microscope objective optical system with adjustable glass cover thickness according to Embodiment 3 of the present invention;

[0093] Fig.28 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the third embodiment of the present invention;

[0094] Fig.29 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the third embodiment of the present invention;

[0095] Fig.30 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 0 mm according to the third embodiment of the present invention;

[0096] Fig.31 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the third embodiment of the present invention;

[0097] Fig.32 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the third embodiment of the present invention;

[0098] Fig.33 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 1.1 mm according to the third embodiment of the present invention;

[0099] Fig.34 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the third embodiment of the present invention;

[0100] Fig.35 This is a field curvature diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the third embodiment of the present invention;

[0101] Fig.36 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.0 mm according to the third embodiment of the present invention;

[0102] Fig.37 This is a spherical aberration diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness is 2.5 mm according to the third embodiment of the present invention;

[0103] Fig.38This is a field curvature diagram of the optical system when the thickness D1 of the microscope objective glass cover plate with adjustable glass cover plate thickness is 2.5 mm according to the third embodiment of the present invention;

[0104] Fig.39 This is a distortion diagram of the optical system when the thickness D1 of the glass cover plate of the microscope objective lens with adjustable glass cover plate thickness according to the third embodiment of the present invention is 2.5 mm. DETAILED DESCRIPTION

[0105] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0106] In the description of the present invention, it should be understood that the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0107] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "set", "put in", "contact", etc. should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or can communicate with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0108] The following describes the microscope objective optical system with adjustable glass cover thickness and the image capturing device equipped with the microscope objective optical system with adjustable glass cover thickness according to the present invention in conjunction with the accompanying drawings of the specification.

[0109] Embodiment 1

[0110] See also Figure 1 As shown, the microscope objective lens OB01 with adjustable glass cover thickness according to the first embodiment of the present invention includes an object plane 700 , a glass cover 701 , a first lens group 81 , a second lens group 82 and a third lens group 83 .

[0111] The thickness of the glass cover 701 along the optical axis is D1.

[0112] The first lens group 81 includes a first lens 711 and a second lens 712 with positive refractive power. The surface of the first lens 711 facing the object is a first surface 7111, and the surface facing the image is a second surface 7112. The surface of the second lens 712 facing the object is a first surface 7121, and the surface facing the image is a second surface 7122.

[0113] The distance between the first surface 7111 of the first lens 711 facing the object side and the glass cover 701 on the optical axis is D2.

[0114] The second lens group 82 includes a third lens 721 with negative refractive power and a fourth lens 722 with positive refractive power. The surface of the third lens 721 facing the object side is a first surface 7211, and the surface facing the image side is a second surface 7212. The surface of the fourth lens 722 facing the object side is a first surface 7221, and the surface facing the image side is a second surface 7222.

[0115] The third lens group 83 includes a fifth lens 731 with positive refractive power, a sixth lens 732 with negative refractive power, a seventh lens 733 with positive refractive power, an eighth lens 734 with negative refractive power, a ninth lens 735 with positive refractive power, and a tenth lens 736 with negative refractive power.

[0116] The surface 7112 of the first lens 711 facing the image side is a receiving surface and is placed in the first lens holder 611 .

[0117] The surface 7122 of the second lens 712 facing the image side is a receiving surface and is placed in the second lens holder 612 .

[0118] The first lens holder 611 and the second lens holder 612 cooperate to ensure the spacing between the first lens 711 and the second lens 721 on the optical axis.

[0119] The third lens 721 is glued with the surface 7212 facing the image side and the surface 7221 facing the object side of the fourth lens 722. After the third lens 721 and the fourth lens 722 are glued, the surface 7221 of the fourth lens 722 facing the image side is used as a receiving surface to be placed in the third-fourth glued lens holder 621. Through the cooperation between the second lens holder 612 and the third-fourth glued lens holder 621, the interval between the second lens 712 and the third lens 721 on the optical axis, that is, the interval D3 between the first lens group and the second lens group on the optical axis, is ensured.

[0120] The fifth lens 731 is glued with the surface 7312 facing the image side and the surface 7321 facing the object side of the sixth lens 732. After the fifth lens 731 and the sixth lens 732 are glued, the surface 7322 of the sixth lens 732 facing the image side is used as a receiving surface to be placed in the fifth and sixth glued lens holder 631. Through the cooperation between the third and fourth glued lens holders 621 and the fifth and sixth glued lens holders 631, the interval between the fourth lens 722 and the fifth lens 731 on the optical axis, that is, the interval D4 between the second lens group and the third lens group on the optical axis is ensured.

[0121] Zooming is achieved by cooperating between the intervals D3 and D4 to adapt to different thicknesses D1 of the glass cover 701, and the clearest imaging position is achieved by adjusting the interval D2.

[0122] The seventh lens 733 is glued with its surface 7332 facing the image side and the surface 7341 facing the object side of the eighth lens 734. After the seventh lens 733 and the eighth lens 734 are glued, the surface 7331 of the seventh lens 733 facing the object side is used as a receiving surface to be placed in the seventh-eighth glued lens holder 632. Through the cooperation of the fifth-sixth glued lens holder 631 and the seventh-eighth glued lens holder 632, the spacing between the sixth lens 732 and the seventh lens 733 on the optical axis is ensured.

[0123] The ninth lens 735 is glued with the surface 7352 facing the image side and the surface 7361 facing the object side of the tenth lens 736. After the ninth lens 735 and the tenth lens 736 are glued, the surface 7362 facing the image side of the tenth lens 736 is used as the receiving surface to be placed in the ninth and tenth glued lens holder 633. Through the cooperation of the seventh and eighth glued lens holders 632 and the ninth and tenth glued lens holders 633, the spacing between the eighth lens 734 and the ninth lens 735 on the optical axis is guaranteed.

[0124] In the optical system of the microscope objective lens with adjustable glass cover thickness of the present embodiment, the field of view is 25 mm and the numerical aperture NA is 0.45.

[0125] Specifically, the focal length f11 of the first lens 711 is 26.14, the refractive index N11 is 1.80, the Abbe number V11 is 39.6, and the thickness T11 is 3.09.

[0126] The second lens 712 has a focal length f12 of 34.48, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 3.40.

[0127] The third lens 721 has a focal length f21 of -24.22, a refractive index N21 of 1.73, an Abbe number V21 of 50.2, and a thickness T21 of 1.00.

[0128] The fourth lens 722 has a focal length f22 of 17.15, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T22 of 5.03.

[0129] The fifth lens 731 has a focal length f31 of 15.92, a refractive index N31 of 1.50, an Abbe number V31 of 81.6, and a thickness T31 of 4.90.

[0130] The sixth lens 732 has a focal length f32 of -21.99, a refractive index N32 of 1.64, an Abbe number V32 of 29.8, and a thickness T32 of 1.00.

[0131] The seventh lens 733 has a focal length f33 of 13.77, a refractive index N33 of 1.50, an Abbe number V33 of 81.6, and a thickness T33 of 4.28.

[0132] The eighth lens 734 has a focal length f34 of -7.96, a refractive index N34 of 1.90, an Abbe number V34 of 24.1, and a thickness T34 of 2.42.

[0133] The focal length f35 of the ninth lens 735 is 11.21, the refractive index N35 is 1.95, the Abbe number V35 is 17.9, and the thickness T35 is 2.98.

[0134] The focal length f36 of the tenth lens 736 is -8.32, the refractive index N36 is 1.49, the Abbe number V36 is 70.4, and the thickness T36 is 1.00.

[0135] Other optical parameters of the microscope objective lens of this embodiment are shown in Table 1-1 and Table 1-2.

[0136] As can be seen from the above, in the microscope objective optical system OB01 with adjustable glass cover thickness of this embodiment, the focal length of the first lens group 81 is the combined focal length of the first lens 711 to the second lens 712, that is, f1 is 14.55; the focal length of the second lens group 82 is the combined focal length of the third lens 721 to the fourth lens 722, that is, f2 is 51.34; the focal length of the third lens group 83 is the combined focal length of the fifth lens 731 to the tenth lens 736, that is, f3 is -37.95.

[0137] Then |f2 / f1| is 0.28, |f3 / f1| is 0.38, |f3 / f2| is 0.74, f11 / f1 is 1.80, f12 / f2 is 2.37, f21 / f2 is -0.47, f22 / f2 is 0.33, f31 / f3 is -0.42, f32 / f3 is 0.58, f33 / f3 is -0.36, f34 / f3 is 0.21, f35 / f3 is -0.30, and f36 / f3 is 0.22. The objective optical system within the focal length numerical range has a large positive refractive power, which makes the microscope objective with adjustable glass cover thickness have a large numerical aperture, and at the same time, it also makes it possible to better control distortion and field curvature.

[0138] In this embodiment, T11 / T12 is 0.91, T21 / T22 is 0.20, T31 / T32 is 4.90, T33 / T34 is 1.77, and T35 / T36 is 2.98.

[0139] Table 1-1

[0140] Radius of curvature Thickness / interval Refractive Index Abbe number focal length Physical surface unlimited 0.00 1.00 Glass cover First side unlimited D1 1.52 59.5 unlimited Side 2 unlimited D2 First lens First side 16.17 3.09 1.80 39.6 26.14 Side 2 9.950 0.80 Second lens First side -17.940 3.40 1.50 81.6 34.48 Side 2 384.243 D3 The third lens First side -44.967 1.00 1.73 50.2 -24.22 Side 2 -12.629 0.00 The fourth lens First side -12.629 5.03 1.50 81.6 17.15 Side 2 22.959 D4 Fifth lens First side -24.899 4.90 1.50 81.6 15.92 Side 2 10.883 0.00 The sixth lens First side 10.883 1.00 1.64 29.8 -21.99 Side 2 48.209 0.80 Seventh lens First side -11.25 4.28 1.50 81.6 13.77 Side 2 15.37 0.00 The eighth lens First side 15.37 2.42 1.90 24.1 —7.96 Side 2 -14.83 1.82 The ninth lens First side 24.969 2.98 1.95 17.94 13.77 Side 2 7.948 0.00 Tenth lens First side 7.948 1.00 1.49 70.4 —7.96 Side 2 -8.688

[0141] Table 1-2

[0142] Thickness / interval Thickness / interval Thickness / interval Thickness / interval D1 0 1.1 2 2.5 D2 10.31 9.57 8.96 8.63 D3 2.37 1.71 1.13 0.80 D4 0.80 1.46 2.04 2.37

[0143] See also Figures 2 to 13 As shown, the aberration diagrams and MTF performance diagrams of the optical system of the microscope objective lens with adjustable glass cover thickness in the first embodiment of the present invention are at different glass cover thicknesses. The various aberrations presented show the resolution capability. When the aberrations are relatively small, images with better quality can be observed.

[0144] Specifically, Figure 2 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 2, wherein the horizontal axis is the spherical aberration amount, in mm, and the vertical axis is the image height, in mm. Referring to Figure 2, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0145] Figure 3The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 3, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 3, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0146] Figure 4 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 4, where the abscissa is the distortion amount, in %, and the ordinate is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0147] Figure 5 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 5, wherein the abscissa is the spherical aberration amount in mm, and the ordinate is the image height in mm. Referring to Figure 5, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0148] Figure 6 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 6, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 6, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0149] Figure 7The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 7, where the abscissa is the distortion amount in %, and the ordinate is the image height in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0150] Figure 8 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 8, wherein the horizontal coordinate is the spherical aberration amount, in mm, and the vertical coordinate is the image height, in mm. Referring to Figure 8, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0151] Fig. 9 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 9, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 9, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0152] Fig.10 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 10, wherein the abscissa is the distortion amount in %, and the ordinate is the image height in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0153] Fig.11The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 11, wherein the abscissa is the spherical aberration amount in mm, and the ordinate is the image height in mm. Referring to Figure 11, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0154] Fig.12 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 12, wherein the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 12, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0155] Fig.13 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the first embodiment of the present invention is shown in Figure 13, wherein the abscissa is the distortion amount, in %, and the ordinate is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0156] According to another aspect of the present invention, an image capture device is equipped with the microscope objective optical system with adjustable glass cover plate thickness, which is used to acquire sample images. Specifically, the specific structure and parameter settings of the microscope objective with adjustable glass cover plate thickness are the same as above, and will not be repeated here.

[0157] Embodiment 2

[0158] As shown in FIG14 , the structure of the microscope objective optical system OB02 with adjustable glass cover thickness in the second embodiment is similar to that in the first embodiment, and also includes a first lens group 81, a second lens group 82, and a third lens group 83; wherein, the first lens group 81 includes a first lens 711 and a second lens 712, the second lens group 82 includes: a second lens 721 and a third lens 722, and the third lens group 83 includes a fifth lens 731, a sixth lens 732, a seventh lens 733, an eighth lens 734, a ninth lens 735, and a tenth lens 736, and the optical parameters of each lens are slightly different from those in the first embodiment.

[0159] The definitions of D1, D2, D3 and D4 are the same as those in the first embodiment, but the values ​​of D2, D3 and D4 can be adjusted accordingly.

[0160] Specifically, in the microscope objective optical system with adjustable glass cover thickness of the second embodiment, the field of view is 25 mm, and the numerical aperture NA is 0.4.

[0161] The focal length f11 of the first lens 711 is 26.33, the refractive index N11 is 1.80, the Abbe number V11 is 39.6, and the thickness T11 is 3.09.

[0162] The second lens 712 has a focal length f12 of 34.59, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 4.46.

[0163] The third lens 721 has a focal length f21 of -24.39, a refractive index N21 of 1.73, an Abbe number V21 of 48.8, and a thickness T21 of 1.00.

[0164] The fourth lens 722 has a focal length f22 of 17.15, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T22 of 4.34.

[0165] The fifth lens 731 has a focal length f31 of 15.67, a refractive index N31 of 1.50, an Abbe number V31 of 81.6, and a thickness T31 of 4.69.

[0166] The sixth lens 732 has a focal length f32 of -21.64, a refractive index N32 of 1.64, an Abbe number V32 of 30.3, and a thickness T32 of 1.00.

[0167] The seventh lens 733 has a focal length f33 of 13.77, a refractive index N33 of 1.50, an Abbe number V33 of 81.6, and a thickness T33 of 4.28.

[0168] The eighth lens 734 has a focal length f34 of -7.91, a refractive index N34 of 1.90, an Abbe number V34 of 24.2, and a thickness T34 of 2.44.

[0169] The focal length f35 of the ninth lens 735 is 11.03, the refractive index N35 is 1.95, the Abbe number V35 is 17.9, and the thickness T35 is 2.80.

[0170] The focal length f36 of the tenth lens 736 is -8.29, the refractive index N36 is 1.49, the Abbe number V36 is 70.4, and the thickness T36 is 1.08.

[0171] Other optical parameters of the microscope objective lens with adjustable glass cover thickness of this embodiment are shown in Tables 2-1 and 2-2.

[0172] As can be seen from the above, in the microscope objective optical system OB02 with adjustable glass cover thickness of the present embodiment, the focal length of the first lens group 81 is the combined focal length of the first lens 711 to the second lens 712, that is, f1 is 14.55; the focal length of the second lens group 82 is the combined focal length of the third lens 721 to the fourth lens 722, that is, f2 is 51.34; the focal length of the third lens group 83 is the combined focal length of the fifth lens 731 to the tenth lens 736, that is, f3 is -37.95.

[0173] Then |f2 / f1| is 0.29, |f3 / f1| is 0.38, |f3 / f2| is 0.76, f11 / f1 is 1.80, f12 / f2 is 2.36, f21 / f2 is -0.47, f22 / f2 is 0.33, f31 / f3 is -0.40, f32 / f3 is 0.56, f33 / f3 is -0.35, f34 / f3 is 0.20, f35 / f3 is -0.28, and f36 / f3 is 0.21. The objective optical system within the focal length numerical range has a large positive refractive power, which makes the microscope objective with adjustable glass cover thickness have a large numerical aperture, and at the same time, it also makes it possible to better control distortion and field curvature.

[0174] In this embodiment, T11 / T12 is 0.69, T21 / T22 is 0.23, T31 / T32 is 4.69, T33 / T34 is 1.76, and T35 / T36 is 2.60.

[0175] Table 2-1

[0176] Radius of curvature Thickness / interval Refractive Index Abbe number focal length Physical surface unlimited 0.00 1.00 Glass cover First side unlimited D1 1.52 59.5 unlimited Side 2 unlimited D2 First lens First side 16.18 3.09 1.80 39.6 26.33 Side 2 9.984 0.88 Second lens First side -18.183 4.46 1.50 81.6 34.59 Side 2 306.180 D3 The third lens First side -48.895 1.00 1.73 48.8 -24.39 Side 2 -12.990 0.00 The fourth lens First side -12.990 4.34 1.50 81.6 17.15 Side 2 22.243 D4 Fifth lens First side -24.950 4.69 1.50 81.6 15.67 Side 2 10.658 0.00 The sixth lens First side 10.658 1.00 1.64 30.3 -21.64 Side 2 46.574 0.80 Seventh lens First side -11.23 4.28 1.50 81.6 13.77 Side 2 15.42 0.00 The eighth lens First side 15.42 2.44 1.90 24.2 -7.91 Side 2 -14.58 1.65 The ninth lens First side 24.529 2.80 1.95 17.94 11.03 Side 2 7.798 0.00 Tenth lens First side 7.798 1.08 1.49 70.4 -8.29 Side 2 -8.821

[0177] Table 2-2

[0178] Thickness / interval Thickness / interval Thickness / interval Thickness / interval D1 0 1.1 2 2.5 D2 10.31 9.57 8.96 8.63 D3 2.37 1.71 1.13 0.80 D4 0.80 1.46 2.04 2.37

[0179] See Figure Figures 15 to 26 The aberration diagrams and MTF performance diagrams of the microscope objective optical system with adjustable glass cover plate thickness of this embodiment under different glass cover plate thicknesses are shown. The various aberrations presented therein represent the resolution capability. When the aberrations are relatively small, images with better quality can be observed.

[0180] Fig.15The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the embodiment of the present invention is shown in Figure 15, wherein the abscissa is the spherical aberration amount, in mm, and the ordinate is the image height, in mm. Referring to Figure 15, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0181] Fig.16 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 16, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 16, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0182] Fig.17 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 17, wherein the abscissa is the distortion amount, in %, and the ordinate is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0183] Fig.18 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 18, wherein the horizontal coordinate is the spherical aberration amount, in mm, and the vertical coordinate is the image height, in mm. Referring to Figure 18, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0184] Fig.19The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 19, wherein the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 19, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0185] Fig. 20 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in 20, wherein the abscissa is the distortion amount, in %, and the ordinate is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0186] Fig.21 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 21, wherein the abscissa is the spherical aberration amount in mm, and the ordinate is the image height in mm. Referring to Figure 21, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0187] Fig. 22 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG22 , wherein the horizontal axis is the object plane movement amount, in mm, and the vertical axis is the image height, in mm. Referring to FIG22 , the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0188] Fig.23The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG23 , wherein the horizontal axis is the distortion amount, in %, and the vertical axis is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0189] Fig.24 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG24 , wherein the horizontal coordinate is the spherical aberration amount, in mm, and the vertical coordinate is the image height, in mm. Referring to FIG24 , the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0190] Fig.25 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG25 , wherein the horizontal axis is the object plane movement amount, in mm, and the vertical axis is the image height, in mm. Referring to FIG25 , the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0191] Fig.26 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG26 , wherein the horizontal axis is the distortion amount, in %, and the vertical axis is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0192] According to another aspect of the present invention, an image capture device is equipped with the microscope objective optical system with adjustable glass cover plate thickness, which is used to acquire sample images. Specifically, the specific structure and parameter settings of the microscope objective with adjustable glass cover plate thickness are the same as above, and will not be repeated here.

[0193] Embodiment 3

[0194] As shown in 27, the structure of the microscope objective optical system OB03 with adjustable glass cover thickness in this embodiment is similar to that in the first embodiment, and also includes a first lens group 81, a second lens group 82, and a third lens group 83, wherein the first lens group 81 includes a first lens 711 and a second lens 712, the second lens group 82 includes: a second lens 721, a third lens 722, and the third lens group 83 includes a fifth lens 731, a sixth lens 732, a seventh lens 733, an eighth lens 734, a ninth lens 735, and a tenth lens 736, and the optical parameters of each lens are slightly different from those in the first embodiment.

[0195] The definitions of D1, D2, D3 and D4 are the same as those in the first embodiment, but the values ​​of D2, D3 and D4 can be adjusted accordingly.

[0196] Specifically, in the microscope objective optical system with adjustable glass cover thickness of the present embodiment, the field of view is 25 mm, and the numerical aperture NA is 0.5.

[0197] The focal length f11 of the first lens 711 is 24.83, the refractive index N11 is 1.82, the Abbe number V11 is 42.0, and the thickness T11 is 3.35.

[0198] The second lens 712 has a focal length f12 of 32.23, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 3.87.

[0199] The third lens 721 has a focal length f21 of -27.78, a refractive index N21 of 1.65, an Abbe number V21 of 37.4, and a thickness T21 of 1.00.

[0200] The fourth lens 722 has a focal length f22 of 18.39, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T22 of 5.41.

[0201] The fifth lens 731 has a focal length f31 of 16.78, a refractive index N31 of 1.50, an Abbe number V31 of 81.6, and a thickness T31 of 5.06.

[0202] The sixth lens 732 has a focal length f32 of -23.90, a refractive index N32 of 1.66, an Abbe number V32 of 28.1, and a thickness T32 of 1.00.

[0203] The focal length f33 of the seventh lens 733 is 14.09, the refractive index N33 is 1.50, the Abbe number V33 is 81.6, and the thickness T33 is 4.30.

[0204] The eighth lens 734 has a focal length f34 of -8.75, a refractive index N34 of 1.89, an Abbe number V34 of 26.5, and a thickness T34 of 1.00.

[0205] The ninth lens 735 has a focal length f35 of 12.65, a refractive index N35 of 1.95, an Abbe number V35 of 17.9, and a thickness T35 of 3.03.

[0206] The focal length f36 of the tenth lens 736 is -8.68, the refractive index N36 is 1.49, the Abbe number V36 is 70.4, and the thickness T36 is 1.00.

[0207] Other optical parameters of the microscope objective optical system with adjustable glass cover thickness in this embodiment are shown in Tables 3-1 and 3-2.

[0208] From the above, it can be seen that in the microscope objective optical system OB03 with adjustable glass cover thickness of this embodiment, the focal length of the first lens group 81 is the combined focal length of the first lens 711 to the second lens 712, that is, f1 is 13.89; the focal length of the second lens group 82 is the combined focal length of the third lens 721 to the fourth lens 722, that is, f2 is 50.12; the focal length of the third lens group 83 is the combined focal length of the fifth lens 731 to the tenth lens 736, that is, f3 is -30.50.

[0209] Then |f2 / f1| is 0.28, |f3 / f1| is 0.46, |f3 / f2| is 0.61, f11 / f1 is 1.76, f12 / f2 is 2.32, f21 / f2 is -0.55, f22 / f2 is 0.37, f31 / f3 is -0.55, f32 / f3 is 0.78, f33 / f3 is -0.46, f34 / f3 is 0.29, f35 / f3 is -0.41, and f36 / f3 is 0.28. The objective optical system within the focal length numerical range has a large positive refractive power, which makes the microscope objective with adjustable glass cover thickness have a large numerical aperture, and at the same time, it also makes it possible to better control distortion and field curvature.

[0210] In the third embodiment, T11 / T12 is 0.87, T21 / T22 is 0.18, T31 / T32 is 5.06, T33 / T34 is 4.30, and T35 / T36 is 3.03.

[0211] Table 3-1

[0212] Radius of curvature Thickness / interval Refractive Index Abbe number focal length Physical surface unlimited 0.00 1.00 Glass cover First side unlimited 0.00 1.52 59.5 unlimited Side 2 unlimited 10.03 First lens First side 18.36 3.35 1.82 42.0 24.83 Side 2 10.447 0.80 Second lens First side -21.224 3.87 1.50 81.6 32.23 Side 2 62.090 2.68 The third lens First side -39.468 1.00 1.65 37.4 -27.78 Side 2 -12.311 0.00 The fourth lens First side -12.311 5.41 1.50 81.6 18.39 Side 2 30.628 0.80 Fifth lens First side -23.795 5.06 1.50 81.6 16.78 Side 2 11.981 0.00 The sixth lens First side 11.981 1.00 1.66 28.1 -23.90 Side 2 49.622 0.80 Seventh lens First side -13.77 4.30 1.50 81.6 14.09 Side 2 12.85 0.00 The eighth lens First side 12.85 1.00 1.89 26.5 —8.75 Side 2 -20.78 1.87 The ninth lens First side 21.380 3.03 1.95 17.94 12.65 Side 2 8.273 0.00 Tenth lens First side 8.273 1.00 1.49 70.4 —8.68 Side 2 -9.075

[0213] Table 3-2

[0214] Thickness / interval Thickness / interval Thickness / interval Thickness / interval D1 0 1.1 2 2.5 D2 10.03 9.27 8.64 8.30 D3 2.68 1.89 1.20 0.80 D4 0.80 1.59 2.28 2.68

[0215] Figures 28 to 39The aberration diagrams and MTF performance diagrams of the microscope objective optical system with adjustable glass cover thickness of the third embodiment under different glass cover thicknesses are shown. The various aberrations presented show the resolution capability. When the aberrations are relatively small, images with better quality can be observed.

[0216] Fig.28 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 28, wherein the horizontal axis is the spherical aberration amount, in mm, and the vertical axis is the image height, in mm. Referring to Figure 28, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0217] Fig.29 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 29, wherein the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 29, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0218] Fig.30 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG30 , wherein the horizontal axis is the distortion amount, in %, and the vertical axis is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0219] Fig.31 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 31, wherein the horizontal coordinate is the spherical aberration amount, in mm, and the vertical coordinate is the image height, in mm. Referring to Figure 31, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0220] Fig.32The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 32, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 32, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0221] Fig.33 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in FIG33 , wherein the horizontal axis is the distortion amount, in %, and the vertical axis is the image height, in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0222] Fig.34 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 34, where the horizontal coordinate is the spherical aberration amount, in mm, and the vertical coordinate is the image height, in mm. Referring to Figure 34, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0223] Fig.35 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 35, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 35, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0224] Fig.36The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 36, where the abscissa is the distortion amount in %, and the ordinate is the image height in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0225] Fig.37 The spherical aberration diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 37, wherein the horizontal axis is the spherical aberration amount, in mm, and the vertical axis is the image height, in mm. Referring to Figure 37, the solid line represents the d line, the dotted line represents the C line, the single-point dash line represents the F line, and the double-point dash line represents the g line. The spherical aberration of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.010 mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0226] Fig.38 The field curvature diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 38, where the horizontal axis is the object plane movement, in mm, and the vertical axis is the image height, in mm. As shown in Figure 38, the solid line represents the sagittal relative to each wavelength of light, and the dotted line represents the meridian relative to each wavelength. From the distribution of the field curvature, it can be seen that the field curvature of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±0.005mm, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0227] Fig.39 The distortion diagram of the optical system of the microscope objective lens with adjustable glass cover plate thickness D1 of the second embodiment of the present invention is shown in Figure 39, where the abscissa is the distortion amount in %, and the ordinate is the image height in mm. From the distribution of the distortion, it can be seen that the distortion of the microscope objective lens optical system with adjustable glass cover plate thickness is controlled within ±2%, so that the center resolution of the microscope objective lens optical system with adjustable glass cover plate thickness is optimal.

[0228] According to another aspect of the present invention, an image capture device is provided, which is equipped with an optical system of the microscope objective lens with adjustable glass cover plate thickness, and is used to acquire a sample image. Specifically, the specific structure and parameter settings of the microscope objective lens with adjustable glass cover plate thickness are the same as above, and will not be repeated here.

[0229] The above is only a specific implementation of the present invention, but the design concept of the present invention is not limited to this. Any non-substantial changes to the present invention using this concept shall be deemed as an infringement of the protection scope of the present invention.

Claims

1. A microscope objective lens with adjustable glass cover thickness, characterized in that: include: A first lens group with positive refractive power, a second lens group with positive refractive power, and a third lens group with negative refractive power are coaxially arranged in sequence from the object direction to the image side; The microscope objective lens with adjustable glass cover thickness meets the following conditions: 0.16<|f2 / f1|<2.15; 0.10<|f3 / f1|<2.16; 0.20<|f3 / f2|<2.19; Wherein, f1 represents the focal length of the first lens group; f2 represents the focal length of the second lens group; f3 represents the focal length of the third lens group; The first lens group is closest to the object side and includes a first lens and a second lens, the first lens has positive refractive power, and the second lens has positive refractive power; The second lens group includes one or more cemented lenses, specifically, a third lens and a fourth lens are sequentially arranged from the object direction to the image direction; the third lens has a negative refractive power, and the fourth lens has a positive refractive power; the second lens group is a focusing group, and the change of the thickness of the glass cover plate is adapted by changing the optical interval between the second lens group and the first lens group and the third lens group; The third lens group includes two or more cemented lenses, specifically, a fifth lens, a sixth lens, a seventh lens, an eighth lens, a ninth lens and a tenth lens are sequentially arranged from the object direction to the image side; the fifth lens has a positive refractive power, the sixth lens has a negative refractive power, the seventh lens has a negative refractive power, the eighth lens has a negative refractive power, the ninth lens has a negative refractive power, and the tenth lens has a negative refractive power; The first lens group, the second lens group and the third lens group satisfy the following conditional formula: 0.6 <f11 / f1<3.0; 1.2 <f12 / f1<3.6; -1.7 <f21 / f2<0.7; -0.9 <f22 / f2<1.5; -1.6 <f31 / f3<0.8; -0.6 <f32 / f3<1.8; -1.6 <f33 / f3<0.8; -1.0 <f34 / f3<1.4; -1.5 <f35 / f3<0.9; -1.0 <f36 / f3<1.4; Among them, f11 is the focal length of the first lens, f12 is the focal length of the second lens, f21 is the focal length of the third lens, f22 is the focal length of the fourth lens, f31 is the focal length of the fifth lens, f32 is the focal length of the sixth lens, f33 is the focal length of the seventh lens, f34 is the focal length of the eighth lens, f35 is the focal length of the ninth lens, and f36 is the focal length of the tenth lens.

2. The microscope objective lens with adjustable glass cover thickness according to claim 1, characterized in that: The refractive indexes of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens and the tenth lens satisfy the following conditions: 1.8≤N11≤1.95； 1.40≤N12≤1.52； 1.60≤N21≤1.73； 1.40≤N22≤1.50； 1.40≤N31≤1.50； 1.50≤N32≤1.66； 1.40≤N33≤1.50； 1.89≤N34≤2.00； 1.95≤N35≤2.01； 1.40≤N36≤1.49； Among them, N11 is the refractive index of the first lens, N12 is the refractive index of the second lens, N21 is the refractive index of the third lens, N22 is the refractive index of the fourth lens, N31 is the refractive index of the fifth lens, N32 is the refractive index of the sixth lens, N33 is the refractive index of the seventh lens, N34 is the refractive index of the eighth lens, N35 is the refractive index of the ninth lens, and N36 is the refractive index of the tenth lens.

3. The microscope objective lens with adjustable glass cover thickness according to claim 1, characterized in that: The Abbe numbers of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens, and the tenth lens satisfy the following conditions: 30≤V11≤42.0； 81.6≤V12≤95； 35≤V21≤50.2； 81.6≤V22≤95； 81.6≤V31≤95； 20≤V32≤30.3； 81.6≤V33≤95； 15≤V34≤26.5； 15≤V35≤17.94； 70.4≤V36≤95； Among them, V11 is the Abbe number of the first lens, V12 is the Abbe number of the second lens, V21 is the Abbe number of the third lens, V22 is the Abbe number of the fourth lens, V31 is the Abbe number of the fifth lens, V32 is the Abbe number of the sixth lens, V33 is the Abbe number of the seventh lens, V34 is the Abbe number of the eighth lens, V35 is the Abbe number of the ninth lens, and V36 is the Abbe number of the tenth lens.

4. The microscope objective lens with adjustable glass cover thickness according to claim 1, characterized in that: The thicknesses of the first lens, the second lens, the third lens, the fourth lens, the fifth lens, the sixth lens, the seventh lens, the eighth lens, the ninth lens, and the tenth lens meet the following conditions: 0.69≤T11 / T12≤0.91; 0.1 <T21 / T22<0.6; 3.0 <T31 / T32<8.0; 1.0 <T33 / T34<5.0; 1.5 <T35 / T36<4.0; Wherein, T11 is the thickness of the first lens on the optical axis, T12 is the thickness of the second lens on the optical axis, T21 is the thickness of the third lens on the optical axis, T22 is the thickness of the fourth lens on the optical axis, T31 is the thickness of the fifth lens on the optical axis, T32 is the thickness of the sixth lens on the optical axis, T33 is the thickness of the seventh lens on the optical axis, T34 is the thickness of the eighth lens on the optical axis, T35 is the thickness of the ninth lens on the optical axis, and T36 is the thickness of the tenth lens on the optical axis.

5. The microscope objective lens with adjustable glass cover thickness according to claim 1, characterized in that: The surface of the third lens facing the image side is cemented with the surface of the fourth lens facing the object side; the surface of the fifth lens facing the image side is cemented with the surface of the sixth lens facing the object side; The surface of the seventh lens facing the image side is cemented with the surface of the eighth lens facing the object side; The surface of the ninth lens facing the image side is cemented with the surface of the tenth lens facing the object side.

6. An image capturing device, characterized in that: The invention comprises a microscope objective lens with adjustable glass cover thickness as claimed in any one of claims 1 to 5.

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