A multi-axial motion control cell stretching device capable of achieving double-axial motion

Abstract

The application provides a cell stretching device for realizing biaxial movement through multi-axial movement control, and relates to the technical field of biomechanical engineering. The main body comprises a stretching chamber, a motorized telescopic rod is fixed at the center of the rear side of the stretching chamber, and an arc-shaped moving slide plate is connected to the front side of the motorized telescopic rod, three sliding guide rails are fixed to the bottom of the moving slide plate, and three moving blocks are respectively fixed on the three sliding blocks. The moving blocks at the middle part are fixed together with the moving slide plate to make reciprocating movement, so that the movement of one point of the cell stretching cavity is realized. The ends of the moving blocks at the two sides are fixed with two bearing rods, bearings are fixed on the bearing rods, the bearings can move back and forth in the slot of the moving slide plate, and the movement of the two points of the cell stretching cavity is realized. The remaining point is fixed on the fixed block at the front end of the bottom plate. Through the movement of the three points, the biaxial synchronous stretching of the cell stretching cavity is realized, and the movement directions of the three points are fixed on the corresponding axial directions by the motorized telescopic rod and the guide rail, so that the stretching direction is ensured.

Description

Technical Field

[0001] This invention relates to the field of biomechanical engineering technology, and in particular to a cell stretching device that achieves biaxial motion control through multiaxial motion control. Background Technology

[0002] Existing technologies have established various mechanical culture methods for cells, applying mechanical stimulation to cells, including tensile tension, pressure, and fluid shear force. Tensile stimulation devices mainly adopt the substrate strain loading method: the substrate is an elastic membrane material, and displacement or pressure is applied to it, causing recoverable elastic deformation, thereby subjecting the cells on the membrane to corresponding tensile strain stimulation.

[0003] However, most existing devices can only perform motion control in one axis, or require control by multiple motors, and can only stretch one cell stretching cavity. Their structures are complex and cannot be used for control experiments. Summary of the Invention

[0004] The purpose of this invention is to solve the problem that most existing devices require control by multiple motors and can only stretch one cell stretching cavity, resulting in complex structures and the inability to conduct control experiments. The invention proposes a novel multi-axial motion control scheme to ultimately achieve biaxial motion.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a cell stretching device for achieving biaxial motion control via multi-axial motion, comprising a stretching chamber, characterized in that the stretching chamber comprises a stretching chamber shell and an internal stretching structure; the stretching chamber shell comprises a base plate, a rear cover, and a front cover with an observation window; the base plate, front cover, and rear cover constitute a hollow shell, and the internal stretching structure is disposed inside the stretching chamber shell; the internal stretching structure comprises an electric telescopic rod fixed to the base plate and a stretching module for achieving stretching;

[0006] The stretching module includes a movable slide plate connected to an electric telescopic rod, two movable blocks A, one movable block B, a fixed block, and several stacked cell stretching cavities. Each of the fixed block, the two movable blocks A, and the one movable block B is equipped with a column. The line connecting the four columns forms a square, and the four columns are used to fix the four corners of the cell stretching cavities. The fixed block is fixed to the side of the base plate away from the electric telescopic rod. The two movable blocks A and the one movable block B are slidably mounted on three sliding guide rails. The sliding guide rail with movable block B and the fixed block are both mounted on the axis of the electric telescopic rod. The two sliding guide rails with movable blocks A are symmetrically arranged on both sides of the axis of the electric telescopic rod. The movable slide plate has symmetrically arranged side arms at both ends, and the middle of the movable slide plate is fixedly connected to the telescopic end of the electric telescopic rod. Both sides of the movable slide plate have oblong holes. The two movable blocks A are respectively movably fitted into the oblong holes of the two sides of the movable slide plate, and the movable block B is fixedly connected to the middle of the movable slide plate.

[0007] As a preferred embodiment of the present invention, the movable block A includes a slider disposed on a sliding guide rail, a column fixed to one end of the slider, and a bearing assembly fixed to the other end of the slider; the bearing assembly includes a bearing rod fixed to the slider and a bearing disposed on the bearing rod, the bearing rod being movably sleeved in the side arm waist-shaped hole of the movable slide plate through the bearing.

[0008] As a preferred embodiment of the present invention, the movable block B includes a slider disposed on a sliding guide rail and a column fixed to one end of the slider; the other end of the slider is connected to the middle of the movable slide plate.

[0009] As a preferred embodiment of the present invention, the line connecting the moving block B and the fixed block is collinear with the axis of the electric telescopic rod.

[0010] As a preferred embodiment of the present invention, the rear cover is located on the side of the base plate where the electric telescopic rod is provided, the rear cover is provided with a display screen that is easy to adjust, and the rear cover is provided with a button for activating the cell stretching device at the side of the display screen.

[0011] As a preferred embodiment of the present invention, the front cover is located on the side of the base plate where the fixing block is provided, and the front cover is hinged to the rear cover.

[0012] As a preferred embodiment of the present invention, the length direction of the waist-shaped hole on the side arm of the movable slide is perpendicular to the moving direction of the corresponding movable block A.

[0013] As a preferred embodiment of the present invention, holes are provided at the four corners of the cell stretching cavity to accommodate the column. After the cell stretching cavity is fitted onto the column, it is fixed by buckles. A cell stretching cavity cover plate is also provided above the cell stretching cavity.

[0014] As a preferred embodiment of the present invention, the tracks of the three sliding guides are all oriented towards the fixed block, so that the shape of the cell stretching cavity can always remain square during the stretching process.

[0015] This invention also provides a method for using a novel multi-axis motion control structure to ultimately achieve bi-axis motion, comprising the following steps:

[0016] 1) Open the front cover, stack several cell stretching cavities, and set the four corners of the cell stretching cavities on two moving blocks A, one moving block B, and one fixed block respectively;

[0017] 2) Connect the cell stretching device to the power supply and set the stretching data on the cell stretching device;

[0018] 3) After setting the data, close the front cover, start the electric telescopic rod, and move the moving slide plate; the moving slide plate then moves two moving blocks A and one moving block B to achieve biaxial stretching of the cell stretching cavity, so that several cell stretching cavities are stretched into a square shape.

[0019] 4) During the stretching process, observe the stretching of the cell stretching cavity through the observation window;

[0020] 5) After observation, reset the cell stretching chamber, turn off the power, open the front cover, and remove the cell stretching chamber.

[0021] Compared with the prior art, the advantages and positive effects of the present invention are as follows:

[0022] 1. In this invention, the extension and retraction of a single electric telescopic rod facilitates the simultaneous reciprocating movement of the moving slide and the moving block. Furthermore, the connection between the cell stretching membrane and the fixed block and the moving block enables biaxial repeated stretching of multiple cell stretching cavities during the reciprocating motion of the moving block. It also facilitates the adjustment of the stretching frequency and amplitude of the cell stretching membrane by adjusting the extension and retraction frequency of the electric telescopic rod. Moreover, the guide rail slider ensures smooth movement of the moving block and prevents deviation in the stretching direction.

[0023] 2. In this invention, by setting the height of the fixed block, the moving block, and the column, multiple cell stretching cavities can be stacked to achieve simultaneous testing of multiple samples and to achieve the purpose of control. Attached Figure Description

[0024] Figure 1 This invention presents a three-dimensional structural schematic diagram of a cell stretching device that achieves biaxial motion control through multi-axial motion control.

[0025] Figure 2 This invention presents a schematic diagram of the internal structure of a cell stretching device that achieves biaxial motion control through multiaxial motion control.

[0026] Figure 3 This invention presents a schematic diagram of a cell stretching device for removing one side of the outer shell, which achieves biaxial motion control through multiaxial motion control.

[0027] Figure 4 This invention presents a schematic diagram of the internal stretching motion structure of a stretching device that achieves biaxial motion through multi-axial motion control.

[0028] Legend: 1. Front cover; 2. Rear cover; 3. Protective cover; 4. Observation window; 5. Display screen; 6. Button; 7. Cell stretching chamber; 8. Base plate; 9. Moving block A; 10. Moving block B; 11. Column; 12. Buckle; 13. Fixing block; 14. Electric telescopic rod; 15. Moving slide plate; 16. Bearing rod; 17. Bearing; 18. Sliding guide rail; 19. Cell stretching chamber cover plate. Detailed Implementation

[0029] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0030] Numerous specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways than those described herein, and therefore the invention is not limited to the specific embodiments disclosed in the following specification.

[0031] Example 1, as Figure 1 and Figure 3 As shown, a cell stretching device for achieving biaxial motion control through multiaxial motion control includes a stretching chamber, which includes a stretching chamber shell and an internal stretching device. The stretching chamber shell includes a bottom plate 8, a rear cover 2, and a front cover 1 with an observation window. The bottom plate 8, the front cover 1, and the rear cover 2 form a hollow shell, and the internal stretching device is disposed inside the stretching chamber shell.

[0032] like Figure 2As described, an electric telescopic rod 14 is fixedly connected to the center of the rear side of the bottom plate 8 of the stretching chamber 1, and a fixing block 13 is fixedly connected to the front side of the bottom plate 8 of the stretching chamber 1. Three sliding guide rails 18 are fixedly fixed at the bottom of the stretching chamber. Two moving blocks A9 and B10 are fixedly connected to the moving slider respectively. A bearing rod 16 is fixedly connected to the moving block A9, and a bearing 17 is connected to the bearing rod 16. Multiple stacked cell stretching cavities 7 are provided at the front ends of the moving blocks A9 and B10. Above the uppermost cell stretching cavity 7, there is also a... A cell stretching chamber cover plate 19 is provided to ensure that the cells are tested in a relatively sealed environment, thereby ensuring that the cells are not damaged by other factors during the test. The movable slide plate 15 is provided with side arms symmetrically arranged at both ends. The middle part of the movable slide plate 15 is fixedly connected to the telescopic end of the electric telescopic rod 14. Both sides of the movable slide plate 15 are provided with waist-shaped holes. The two movable blocks A9 are respectively movably sleeved in the waist-shaped holes of the two sides of the movable slide plate 15. The movable block B10 is fixedly connected to the middle part of the movable slide plate 15.

[0033] In a preferred embodiment of the present invention, the telescopic end of the electric tension rod is fixedly connected to the center of the rear surface of the movable slide plate 15, facilitating the reciprocating movement of the transfer slide plate by extending and retracting the electric telescopic rod 14. The center of the lower surface of the movable slide plate 15 is fixedly connected to the upper rear surface of the movable block B10, facilitating the reciprocating movement of the movable block B10 by the reciprocating movement of the movable slide plate 15. The oblong holes at both ends of the movable slide plate 15 are rolledly connected to the outer ring of the bearing 17, facilitating the reciprocating movement of the bearing 17 within the two slots of the movable slide plate 15 by the reciprocating movement of the movable slide plate 15. The inner ring of the bearing 17 is connected to the bearing rod 16, which is fixed to the upper rear of the movable block A9. The bearing 17, bearing rod 16, movable slide plate, and column 11 are fixedly connected to each other to form a single movable block A9; the movement of the bearing 17 drives the movement of the bearing rod 16, so that the movable block moves together when the bearing rod 16 is moved. Both moving blocks A9 and B10 are fixed on the sliding guide rail 18, ensuring that the moving blocks can only reciprocate along the straight line of the sliding guide rail 18.

[0034] In one embodiment of the present invention, the sliding guide rail 18 is fixed on the base plate 8. The two sliding guide rails 18 with movable blocks A are placed at 90°, and the sliding guide rail 18 with movable blocks B is placed on the axis of the stretching chamber, so that the holes at the four corners of the cell stretching cavity 7 can always remain square. The four corner holes of the cell stretching cavity 7 are respectively embedded in the column 11 and fixed by buckles, which facilitates the quick removal and installation of the cell stretching cavity 7.

[0035] In one embodiment of the present invention, a display screen 5 is provided at the top rear side of the outer surface of the stretching chamber, and a button 6 is provided at the top rear side of one side of the outer surface of the stretching chamber, so as to facilitate personnel to turn the equipment on and off and change various data during stretching.

[0036] The working principle of this invention is that the electric telescopic rod 14 repeatedly extends and retracts, driving the movable slide plate 15 and the movable block B10 to reciprocate. The movable block A9, driven by the bearing 17, also reciprocates. However, the straight line of the sliding guide rail 18 fixed to the movable block A9 forms a 45-degree angle with the straight line of the sliding guide rail 18 fixed to the movable block B10, and the distance of movement differs from that of the movable block B10. Ultimately, this causes the several cell stretching cavities 7 fixed above to be stretched into a square shape. Figure 4 As shown, the left figure shows the size of the cell stretching cavity 7 when the electric telescopic rod 14 is extended, and the right figure shows the size of the cell stretching cavity 7 when the electric telescopic rod 14 is retracted. By changing various data during stretching through the display screen 5, and adjusting the speed and distance of the electric telescopic rod 14, the stretching frequency and distance can be adjusted. The fixed block 13 is fixed, and the moving block A9 is driven by the bearing 17 to make linear reciprocating motion, thereby realizing the stretching motion of the cell stretching membrane.

[0037] To more clearly illustrate the working process of the above-mentioned device, a stretching method for a cell stretching device that achieves biaxial motion through multi-axial motion control is also provided, comprising the following steps:

[0038] 1) Open the front cover, stack several cell stretching cavities 7, and set the four corners of the cell stretching cavities 7 on two moving blocks A9, one moving block B10 and one fixed block 13 respectively.

[0039] 2) Cover the cell stretching cavity 7 with the cell cover 19, and fix the tops of the four pillars 11 to two moving blocks A9, one moving block B10 and one fixed block 13 with the buckles 12;

[0040] 3) Connect the cell stretching device to the power supply and press button 6 to light up the display screen 5. Set various stretching data through the display screen 5. The stretching data includes the extension and retraction speed of the electric telescopic rod 14, the size of the stretching chamber 7, etc.

[0041] 4) After setting the data, close the front cover 1 and start the electric telescopic rod 14 through the display screen 5. The electric telescopic rod 14 drives the moving slide plate 15 to move. The moving slide plate 15 then drives two moving blocks A9 and one moving block B10 to move, realizing the biaxial stretching of the cell stretching cavity 7, so that several cell stretching cavities 7 are stretched into a square shape.

[0042] 5) During the stretching process, the stretching of the cell stretching cavity 7 can be observed through the observation window 4;

[0043] 6) After completion, reset the cell stretching cavity 7 via the display screen 5, press button 6 to cut off the power, open the front cover 1, and remove the cell stretching cavity 7.

[0044] In summary, the present invention provides a novel multi-axial motion control structure that ultimately achieves biaxial motion by extending and retracting a single electric telescopic rod to realize biaxial repeated stretching of multiple cell stretching cavities. Furthermore, by adjusting the extension and retraction frequency of the electric telescopic rod, the stretching frequency and amplitude of the cell stretching membrane can be adjusted more conveniently. Moreover, the set guide rail slider ensures smooth movement of the moving block and guarantees that the stretching direction does not deviate.

[0045] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the protection scope of the present invention.

Claims

1. A multi-axial motion control cell stretching device for achieving biaxial motion, comprising a stretching chamber, characterized in that, The stretching chamber comprises a stretching chamber shell and an internal stretching structure; the stretching chamber shell comprises a bottom plate (8), a rear cover (2), and a front cover (1) provided with an observation window; the bottom plate (8), the front cover (1), and the rear cover (2) constitute an internally hollow shell, and the internal stretching structure is arranged inside the stretching chamber shell; The internal stretching structure comprises a motorized telescopic rod (14) fixed on the bottom plate (8) and a stretching module for realizing stretching; The stretching module comprises a moving slide plate (15) connected with the motorized telescopic rod (14), two moving blocks A (9), one moving block B (10), one fixed block (13), and a plurality of stacked cell stretching cavities (7); the fixed block (13), the two moving blocks A (9), and the one moving block B (10) are all provided with upright columns (11), the connecting lines of the four upright columns (11) can constitute a square, and the four upright columns (11) are used for fixing four corners of the cell stretching cavity (7); the fixed block (13) is fixed on the side of the bottom plate (8) away from the motorized telescopic rod (14); the two moving blocks A (9) and the one moving block B (10) are respectively slidingly arranged on three sliding guide rails (18); the sliding guide rail (18) provided with the moving block B (10) and the fixed block (13) are both arranged on the axis of the motorized telescopic rod (14); the two sliding guide rails (18) provided with the moving blocks A (9) are symmetrically arranged on the two sides of the axis of the motorized telescopic rod (14); the two ends of the moving slide plate (15) are symmetrically provided with side arms, the middle part of the moving slide plate (15) is fixedly connected with the telescopic end of the motorized telescopic rod (14), and the two side arms of the moving slide plate (15) are both provided with waist-shaped holes; the two moving blocks A (9) are respectively movably sleeved in the waist-shaped holes of the two side arms of the moving slide plate (15), and the moving block B (10) is fixedly connected with the middle part of the moving slide plate (15); The length direction of the waist-shaped hole on the side arm of the moving slide plate (15) is perpendicular to the moving direction of the corresponding moving block A (9); The method for realizing the biaxial movement of the device comprises the following steps, 1) opening the front cover, stacking a plurality of cell stretching cavities (7), and arranging the four corners of the cell stretching cavities (7) on the two moving blocks A (9), the one moving block B (10), and the one fixed block (13) respectively; 2) connecting the cell stretching device to the power supply and setting various data of stretching on the cell stretching device; 3) after setting the data, covering the front cover (1), starting the motorized telescopic rod (14), and driving the moving slide plate (15) to move; the moving slide plate (15) drives the two moving blocks A (9) and the one moving block B (10) to move, realizes the biaxial stretching of the cell stretching cavities (7), and makes the plurality of cell stretching cavities (7) be enlarged in the shape of a square; 4) during the stretching process, observing the stretching condition of the cell stretching cavities (7) through the observation window (4); 5) after the observation is completed, resetting the cell stretching cavities (7), cutting off the power supply, opening the front cover (1), and taking out the cell stretching cavities (7).

2. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein, The moving block A (9) comprises a sliding block arranged on the sliding guide, a stand (11) fixed at one end of the sliding block, and a bearing set fixed at the other end of the sliding block; the bearing set comprises a bearing rod (16) fixed on the sliding block and a bearing (17) arranged on the bearing rod (16), and the bearing rod (16) is movably sleeved in the waist-shaped hole of the side arm of the moving slide plate (15) through the bearing (17).

3. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein: The moving block B (10) comprises a sliding block arranged on the sliding guide (18) and a stand (11) fixed at one end of the sliding block; the other end of the sliding block is connected with the middle part of the moving slide plate (15).

4. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein: The line connecting the moving block B (10) and the fixed block (13) is collinear with the axis of the electric telescopic rod (14).

5. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein: The rear cover (2) is located on the side of the bottom plate (8) where the electric telescopic rod (14) is arranged, a display screen (5) for easy control is arranged on the rear cover (2), and a button (6) for starting the cell stretching device is arranged on the side of the rear cover (2) where the display screen (5) is located.

6. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein: The front cover (1) is located on the side of the bottom plate (8) where the fixed block (13) is arranged, and the front cover (1) is hinged to the rear cover (2).

7. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein, Holes capable of sleeving the stand (11) are arranged on the four corners of the cell stretching cavity (7), the cell stretching cavity (7) is sleeved on the stand (11) and then fixed through the buckle (12), and a cell stretching cavity cover plate (19) is further arranged above the cell stretching cavity (7).

8. The multi-axial motion controlled biaxial motion enabled cell stretching device of claim 1, wherein, The tracks of the three sliding guides (18) are all directed to the fixed block (13), so that the shape of the cell stretching cavity (7) can always be kept as a square during the stretching process.

Images