Bacteria concentration detection component, device and method, and electrical equipment
By using working electrodes, auxiliary electrodes and electronic control components for electrolytic detection in bacterial concentration detection, the problem of slow detection speed in the prior art is solved, and a fast and highly adaptable bacterial concentration detection is achieved.
Patent Information
- Application Number
- CN202110650650.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-06-10
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2041-06-10
AI Technical Summary
In the prior art, bacterial concentration detection methods such as plate counting method take a long time and slow detection speed, making it difficult to meet the needs of rapid detection.
A bacterial concentration detection component is adopted, including a working electrode, an auxiliary electrode and an electrical control component. By outputting an excitation signal, the working electrode and the auxiliary electrode are electrolyzed on the solution in the container, detected the electrolytic current and the working electrode potential, and determined the bacterial concentration based on the working electrode potential at the peak time of the electrolytic current.
The rapid speed of bacterial concentration detection is achieved, the detection time is significantly shortened, and the electrolysis process has low requirements for environmental factors and is suitable for a variety of environments.
Smart Images

Figure CN115468993B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of air detection, and in particular to a bacteria concentration detection component, a bacteria concentration detection device, a bacteria concentration detection method and electrical equipment. Background Art
[0002] In the prior art, methods for detecting bacterial concentration include the plate count method, etc. However, the plate count method is time-consuming, generally requiring several days, and the detection speed is very slow. Summary of the invention
[0003] The main purpose of the present invention is to provide a bacteria concentration detection component, aiming to improve the speed of bacteria concentration detection.
[0004] To achieve the above object, the present invention provides a bacteria concentration detection component for detecting the bacteria concentration of a solution in a container, the bacteria concentration detection component comprising:
[0005] A working electrode, configured to be disposed in the container;
[0006] an auxiliary electrode, configured to be disposed in the container; and
[0007] An electric control component is electrically connected to the working electrode and the auxiliary electrode respectively, and is used to output an excitation signal to the working electrode so that the working electrode and the auxiliary electrode electrolyze the solution in the container; and is used to detect the electrolysis current and the working electrode potential of the working electrode, and determine the bacterial concentration of the solution in the container according to the working electrode potential corresponding to the peak moment of the detected electrolysis current.
[0008] In one embodiment, the electronic control component comprises:
[0009] A power supply component, electrically connected to the working electrode and the auxiliary electrode, respectively, and configured to output an excitation signal to the working electrode so that the working electrode and the auxiliary electrode electrolyze the solution in the container;
[0010] A current detection circuit, used for detecting the electrolysis current of the working electrode;
[0011] a voltage detection circuit, used for detecting a working electrode potential of the working electrode; and
[0012] A controller is connected to the power supply assembly and the current detection circuit respectively, and is used to determine the bacterial concentration of the solution in the container according to the working electrode potential corresponding to the peak moment of the detected electrolysis current.
[0013] In one embodiment, the bacteria concentration detection component further comprises:
[0014] A reference electrode is electrically connected to the electronic control component. The reference electrode is used to form a test loop with the working electrode and provide a voltage reference signal for the electronic control component.
[0015] In one embodiment, the excitation signal output by the electronic control component includes a first voltage signal and a second voltage signal output in sequence according to a preset cycle. In multiple consecutive cycles, the amplitude of the first voltage signal in the current cycle increases step by step relative to the amplitude of the first voltage signal in the previous cycle, and the amplitude of the second voltage signal in the current cycle increases step by step relative to the amplitude of the second voltage signal in the previous cycle. The second voltage signal in each cycle is greater than the first voltage signal.
[0016] In one embodiment, the amplitude of the first voltage signal in each cycle remains unchanged or increases linearly according to a preset slope;
[0017] The amplitude of the second voltage signal in each cycle remains unchanged or increases linearly according to a preset slope.
[0018] In one embodiment, the electric control component is used to determine that the bacterial concentration is 1CUF / ml to 10 when the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.270 volts and less than -0.245 volts. 2 CUF / ml between;
[0019] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.285 volts and less than -0.270 volts, the bacterial concentration is determined to be 10 2 CUF / m to 10 8 CUF / m between;
[0020] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.367 volts and less than -0.285 volts, the bacterial concentration is determined to be 10 8 CUF / m to 10 10 CUF / m.
[0021] In one embodiment, the working electrode comprises:
[0022] A housing, wherein a housing cavity and a through hole communicating with the housing cavity are provided inside the housing;
[0023] A metal wire is electrically connected to the electric control component, and the metal wire is arranged in the accommodating cavity.
[0024] In one embodiment, one end of the metal wire is flush with the through hole of the housing or extends out of the through hole by a preset length.
[0025] In one embodiment, the diameter of the cross section of the metal wire is less than or equal to 500 micrometers.
[0026] In one embodiment, the bacteria concentration detection component further comprises:
[0027] A display module, electrically connected to the electronic control component;
[0028] The electronic control component is also used to control the display module to display the detected bacteria concentration.
[0029] The present invention also provides a bacteria concentration detection device, which comprises:
[0030] container;
[0031] A gas delivery device, connected to the container, and used to discharge a first preset volume of the gas to be tested into the container;
[0032] an infusion device, connected to the container, and used to discharge a second preset volume of liquid into the container;
[0033] and the bacteria concentration detection assembly according to any one of claims 1 to 10, wherein the working electrode and the auxiliary electrode of the bacteria concentration detection assembly are arranged in the container;
[0034] The electronic control component of the bacteria concentration detection component is also used to determine the bacteria concentration of the gas to be tested according to the bacteria concentration of the liquid in the container, the first preset volume and the second preset volume.
[0035] In one embodiment, the gas delivery device comprises:
[0036] A gas delivery pipe, one end of which is used to receive the gas to be tested, and the other end of which is connected to the container;
[0037] The air pump is arranged on the gas delivery pipe and is electrically connected to the electronic control component, and is used for delivering the gas to be tested to the container and controlling the volume of the delivered gas to be tested.
[0038] In one embodiment, the infusion device comprises:
[0039] A storage tank for storing the liquid;
[0040] an infusion tube, one end of which is connected to the storage tank and the other end of which is connected to the container;
[0041] The infusion pump is arranged on the infusion tube and is electrically connected to the electronic control component, and is used for controlling the volume of the solution delivered from the storage tank to the container.
[0042] In one embodiment, the bacteria concentration detection device further comprises:
[0043] The liquid discharge device is used to discharge the liquid in the container.
[0044] In one embodiment, the container is provided with a liquid discharge port;
[0045] The liquid discharge device is a liquid discharge valve, which is arranged at the liquid discharge port and is used to control the liquid discharge port to discharge the liquid in the container when it is opened.
[0046] The present invention also provides a method for detecting air bacteria concentration, comprising the following steps:
[0047] discharging a second preset volume of liquid into the container;
[0048] Discharging a first preset volume of air into the container so that the air and the liquid form a mixed solution;
[0049] outputting an excitation signal to the working electrode to electrolyze the mixed solution of air and liquid in the container;
[0050] Obtaining the electrolysis current of the working electrode;
[0051] According to the electrolysis current, obtaining the potential of the working electrode corresponding to the peak moment of the electrolysis current;
[0052] Obtaining the bacterial concentration of the mixed solution according to the potential of the working electrode corresponding to the peak moment of the electrolysis current;
[0053] The bacterial concentration of the air is determined according to the bacterial concentration of the mixed solution, the first preset volume, and the second preset volume.
[0054] In one embodiment, the step of obtaining the bacterial concentration of the mixed solution according to the potential of the working electrode corresponding to the peak moment of the electrolysis current is specifically:
[0055] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.270 volts and less than -0.245 volts, the bacterial concentration of the mixed solution is determined to be 1 CUF / ml to 10 2 CUF / ml between;
[0056] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.285 volts and less than -0.270 volts, the bacterial concentration of the mixed solution is determined to be 10 2 CUF / m to 10 8 CUF / m between;
[0057] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.367 volts and less than -0.285 volts, the bacterial concentration of the mixed solution is determined to be 10 8 CUF / m to 10 10 CUF / m.
[0058] In one embodiment, the diameter of the working electrode is less than 500 microns.
[0059] The present invention further provides an electrical device, which includes the above-mentioned bacteria concentration detection component or the above-mentioned bacteria concentration detection device.
[0060] In one embodiment, the electrical device is an air conditioner, an air purifier, a humidifier or a sterilizer.
[0061] The technical solution of the present invention forms a two-electrode system by setting a working electrode and an auxiliary electrode. An electric control component is set, and the electric control component outputs an excitation signal to the two-electrode system, so that the two-electrode system electrolyzes the solution in the container, and the working electrode generates an electrolysis current. The electric control component detects the electrolysis current on the electrode in real time, and obtains the working electrode potential corresponding to the peak moment of the electrolysis current. At this time, the working electrode potential corresponding to the peak moment of different electrolysis currents, that is, corresponds to different bacterial concentrations. By establishing a corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the bacterial concentration, the working electrode potential corresponding to the peak moment of the electrolysis current is brought into the corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the bacterial concentration to obtain the bacterial concentration. In the present invention, the generation and detection of the electrolysis current and the working electrode potential corresponding to the peak moment of the electrolysis current can all be detected in real time. Therefore, the bacterial concentration detection speed of the present invention is very fast, and at the same time, the requirements of electrolysis for environmental factors are very low, so that the bacterial concentration detection component of the present invention can be used in a variety of environments. BRIEF DESCRIPTION OF THE DRAWINGS
[0062] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.
[0063] Figure 1 It is a structural schematic diagram of an embodiment of a bacteria concentration detection component of the present invention;
[0064] Figure 2 It is a structural schematic diagram of another embodiment of the bacteria concentration detection component of the present invention;
[0065] Figure 3 This is a schematic diagram of the first excitation signal waveform of the bacteria concentration detection component of the present invention;
[0066] Figure 4 A schematic diagram of a second excitation signal waveform of the bacteria concentration detection component of the present invention;
[0067] Figure 5 It is a curve diagram showing the relationship between electrolysis current and working electrode potential;
[0068] Figure 6 It is a curve diagram showing the corresponding relationship between the voltage value of the excitation signal and the bacterial concentration;
[0069] Figure 7 It is a structural schematic diagram of an embodiment of a bacteria concentration detection device of the present invention;
[0070] Figure 8 The present invention is a flow chart of the method for detecting bacterial concentration.
[0071] Description of Figure Numbers:
[0072] Label name Label name 10 Electronic control components 1 211 Through Hole 20 Working electrode 61 Gas Pipe 30 Reference electrode 62 air pump 40 Auxiliary electrode 71 Infusion tube 50 container 72 Infusion Pumps 60 Gas transmission device 73 Storage tank 70 Infusion device A1 The first excitation signal 21 case A2 The second excitation signal 22 Metal Wire
[0073] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION
[0074] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0075] It should be noted that if all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings), if the specific posture changes, the directional indication will also change accordingly.
[0076] If the description of "first", "second", etc. in the present invention is only used for descriptive purposes, and cannot be understood as indicating or suggesting their relative importance, or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. If the description of "A and / or B" is involved in the present invention, it means that it includes solution A or solution B, or includes solution A and solution B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.
[0077] The present invention provides a bacteria concentration detection assembly for detecting the bacteria concentration of a solution in a container 50. The container 50 may be any type of device capable of containing liquid.
[0078] It should be noted that the current bacterial detection technologies mainly include plate counting method, solution impedance method, optical microscope counting method, fluorescence microscope counting method, microfluidic chip method, etc. Among them, the plate counting method generally takes several days to test, and strictly requires aseptic operation. The operation process is cumbersome and labor-intensive. At the same time, factors such as culture conditions have a great influence on the test results. The solution impedance method is relatively expensive, and interference factors such as changes in the culture medium have a great influence on the test results. The optical microscope counting method and the fluorescence microscopy counting method are greatly affected by the environment. The microfluidic chip method has a delicate and complex manufacturing process, high cost, and is mostly disposable. The flow channel size is at the micro-nano level, which is easy to be blocked and has high requirements for the water quality of the test. At present, there is a need for a bacterial concentration detection component with fast detection speed, low requirements for the detection environment, and simple operation.
[0079] In order to solve the above technical problems, refer to Figures 1 to 7 In one embodiment of the present invention, the bacteria concentration detection component includes a working electrode 20, an auxiliary electrode 40 and an electric control component 10, wherein:
[0080] The working electrode 20 is used to be arranged in the container 50 and electrically connected to the electronic control component 10; the auxiliary electrode 40 is used to be arranged in the container 50 and electrically connected to the electronic control component 10; the electronic control component 10 is used to output an excitation signal to the working electrode 20, so that the working electrode 20 and the auxiliary electrode 40 electrolyze the solution in the container 50; and is used to detect the electrolysis current and the working electrode potential of the working electrode 20, and determine the bacterial concentration of the solution in the container 50 according to the working electrode potential corresponding to the peak moment of the detected electrolysis current.
[0081] Reference Figure 5 , Figure 5After applying the excitation signal to the working electrode, the measured electrolysis current of the working electrode and the relationship curve of the working electrode potential, the horizontal coordinate is the electrolysis current, and the vertical coordinate is the working electrode potential. It should be noted that as the electrolysis proceeds, the resistance of the solution increases, so as the potential of the working electrode increases, the electrolysis current first increases and then decreases. Wherein the electrolysis current has a peak value. And for bacterial solutions of different concentrations, the time when the electrolysis current peak value occurs is different. Therefore, the bacterial concentration can be determined according to the peak moment of the electrolysis current. Further, since the potential of the working electrode is always increasing, the potential of the working electrode is different at different times, so the working electrode potential corresponding to the peak value of the electrolysis current can be used to characterize the bacterial concentration.
[0082] Refer to Table 1, which shows the working electrode potentials corresponding to the peak values of different electrolysis currents in actual tests, corresponding to different bacterial concentrations. Each working electrode potential has a corresponding bacterial concentration:
[0083] Table 1:
[0084] Working electrode potential (V) Bacterial concentration (CFU / ml) -0.245 1 -0.250 10 -0.270 <![CDATA[10 2 ]]> -0.275 <![CDATA[10 5 ]]> -0.282 <![CDATA[10 7 ]]> -0.284 <![CDATA[10 8 ]]> -0.33 <![CDATA[10 9 ]]> -0.364 <![CDATA[10 10 ]]>
[0085] Reference Figure 6 , Figure 6 To draw Table 1 into an image, a relationship diagram of the concentration of bacteria and the working electrode potential corresponding to the peak moment of the electrolysis current is formed, wherein the horizontal coordinate is the concentration of bacteria, and the vertical coordinate is the working electrode potential corresponding to the peak moment of the electrolysis current. In other words, as the concentration of bacteria in the solution increases, the working electrode potential corresponding to the peak moment of the electrolysis current gradually shifts negatively. In other words, the greater the concentration of bacteria in the solution, the smaller the working electrode potential corresponding to the peak moment of the electrolysis current. Therefore, the solution is electrolyzed, and the electrolysis current and the working potential are detected. The working electrode potential corresponding to the peak moment of the electrolysis current is obtained. Then, by establishing a corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the concentration of bacteria, and then bringing the obtained working electrode potential corresponding to the peak moment of the electrolysis current into the relationship curve, the bacterial concentration of the solution in the container 50 can be determined.
[0086] In the present embodiment, the working electrode 20 is also called a research electrode or an indicator electrode. The working electrode 20 is the object to be detected, and the current flowing through the working electrode 20 is the electrolysis current, and the potential of the working electrode is the working electrode potential. Among them, the working electrode 20 can be a solid electrode, such as a glassy carbon electrode, a graphite electrode, platinum (Pt), gold (Au), silver (Ag), or lead (Pb). The working electrode 20 can also be a liquid electrode, such as a mercury electrode or an amalgam electrode. In the present embodiment, a solid electrode can be selected, and compared with a liquid electrode, a solid electrode can adapt to more application scenarios and use environments. Improve the adaptability of the bacterial concentration detection component of the present embodiment to the environment.
[0087] The auxiliary electrode 40, also called the counter electrode, forms a polarization loop with the working electrode 20, so that current flows through the working electrode 20, so as to detect the electrolysis current of the working electrode 20. The surface area of the auxiliary electrode 40 is much larger than the working electrode 20, for example, it can be selected to be 5-100 times the cross-sectional area of the working electrode 20, so as to reduce the current density of the auxiliary electrode 40, so that the auxiliary electrode 40 is not or substantially not polarized during the measurement process. The auxiliary electrode 40 can be selected from materials such as platinum (Pt), gold (Au), silver (Ag) that have low resistance and are not easily polarized.
[0088] The electric control component 10 may include a polarization power supply device for providing an excitation signal, a device for detecting the electrolysis current, a voltage detection circuit for detecting the working electrode potential, and a signal processing device that can establish a corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the bacterial concentration, and determine the bacterial concentration of the solution in the container 50 according to the corresponding relationship curve and the working electrode potential corresponding to the detected peak moment of the electrolysis current. The electric control component 10 outputs an excitation signal to the working electrode 20. At this time, the working electrode 20, the solution and the auxiliary electrode 40 form a polarization loop. The electrolysis current and the working electrode potential of the polarization loop are detected simultaneously by the device for detecting the electrolysis current and the voltage detection circuit for detecting the working electrode potential. The working electrode potential corresponding to the peak moment of the electrolysis current is obtained, and the bacterial concentration is determined by combining the corresponding relationship curve between the working electrode potential and the bacterial concentration corresponding to the peak moment of the electrolysis current.
[0089] The electric control component 10 in this embodiment can detect the solution in the container 50 by pulse voltammetry to obtain the electrolysis current corresponding to the solution and the working electrode potential corresponding to the peak moment of the electrolysis current, and bring in the corresponding relationship curve of the working electrode potential corresponding to the peak moment of the electrolysis current, wherein, according to the different excitation signals, the voltammetry can be one or more combinations of step pulse voltammetry, normal pulse voltammetry, reverse pulse voltammetry, differential pulse voltammetry, and square wave voltammetry, which are not limited here. This embodiment can be selected as differential pulse voltammetry, which has a low background current, higher detection sensitivity, and lower detection limit, and can better determine the peak moment of the electrolysis current, and then better determine the working electrode potential corresponding to the peak moment of the electrolysis current, thereby improving the detection accuracy of the bacterial concentration detection of this embodiment.
[0090] The technical solution of the present invention forms a two-electrode system by setting a working electrode 20 and an auxiliary electrode 40. An electric control component 10 is set, and the electric control component 10 outputs an excitation signal to the two-electrode system, so that the two-electrode system electrolyzes the solution in the container 50, and the working electrode 20 generates an electrolysis current. The electric control component 10 detects the electrolysis current on the electrode in real time, and obtains the working electrode potential corresponding to the peak moment of the electrolysis current. At this time, the working electrode potential corresponding to the peak moment of different electrolysis currents corresponds to different bacterial concentrations. By establishing a corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the bacterial concentration, the working electrode potential corresponding to the peak moment of the electrolysis current is brought into the corresponding relationship curve between the working electrode potential corresponding to the peak moment of the electrolysis current and the bacterial concentration to obtain the bacterial concentration. In the present invention, the generation and detection of the electrolysis current and the working electrode potential corresponding to the peak moment of the electrolysis current can all be detected in real time. Therefore, the bacterial concentration detection speed of the present invention is very fast, and at the same time, the requirements of electrolysis for environmental factors are very low, so that the bacterial concentration detection component of the present invention can be used in a variety of environments.
[0091] In some embodiments, the bacteria concentration detection component may also be provided with an electrode cleaning device, so that the electrodes (working electrode 20, auxiliary electrode 40 and reference electrode 30 described below) can be cleaned before or after each bacteria concentration detection to remove impurities from the electrodes. This improves the accuracy of the next bacteria concentration detection. The cleaning device is electrically connected to the electric control component 10 and works under the control of the electric control component 10 (or starts working after receiving the electrode cleaning control signal of the electric control component 10). The electrode cleaning device may be one or a combination of the following cleaning devices.
[0092] The organic cleaning device discharges an organic solvent, such as methanol, into the container 50 to immerse the working electrode 20 and the auxiliary electrode 40 , thereby removing organic adsorbents on the surfaces of the working electrode 20 and the auxiliary electrode 40 .
[0093] The mechanical polishing device first sprays polishing paste, such as diamond polishing paste or alumina polishing paste, onto the working electrode 20 and the auxiliary electrode 40; then pure water is discharged into the container 50 to immerse the working electrode 20 and the auxiliary electrode 40, and ultrasonic waves are emitted to ultrasonically clean the working electrode 20 and the auxiliary electrode 40 in the pure water to remove organic and inorganic adsorbed substances on the surfaces of the working electrode 20 and the auxiliary electrode 40, thereby obtaining clean and fresh surfaces of the working electrode 20 and the auxiliary electrode 40. Moreover, the working electrode 20 and the auxiliary electrode 40 can be ensured to have a high surface finish.
[0094] The electrochemical polishing device discharges a dilute acid, such as dilute sulfuric acid, into the container 50 to immerse the working electrode 20 and the auxiliary electrode 40, and outputs an excitation signal through the electronic control component 10. The working electrode 20 and the auxiliary electrode 40 form a polarization circuit for oxidation-reduction. At this time, the working electrode 20 produces oxygen, and hydrogen is produced on the surface of the auxiliary electrode 40. Hydrogen can reduce the oxide on the surface of the auxiliary electrode 40. Then the electronic control component 10 outputs an inverted excitation signal that is inverse to the excitation signal, so that the working electrode 20 and the auxiliary electrode 40 form a reverse polarization circuit. The working electrode 20 produces hydrogen, and oxygen is produced on the surface of the auxiliary electrode 40. Hydrogen can reduce the oxide on the surface of the working electrode 20. By repeatedly outputting the excitation signal and the inverted excitation signal, and ensuring that the last output signal is the inverted excitation signal, the purpose of reducing the working electrode 20 and the auxiliary electrode 40 is achieved.
[0095] Reference Figure 2 In one embodiment of the present invention, the bacteria concentration detection component further includes:
[0096] The reference electrode 30 is electrically connected to the electronic control component 10 . The reference electrode 30 is used to form a test loop with the working electrode 20 and provide a reference signal for the electronic control component 10 .
[0097] The reference electrode 30 can use an electrode whose electrode potential is accurately known and very stable, such as a silver|silver chloride electrode, a calomel electrode, a hydrogen electrode, etc. This is not limited here. The reference electrode 30, the working electrode 20 and the auxiliary electrode 40 form a three-electrode system. Compared with the two-electrode system, the three-electrode system has higher accuracy. Continue to use the electric control component 10 including a polarization power supply device that provides an excitation signal, a device for detecting the electrolysis current, and a corresponding relationship curve between the working electrode potential and the bacterial concentration corresponding to the peak moment of the electrolysis current, and a signal processing device that determines the bacterial concentration of the solution in the container 50 according to the corresponding relationship curve and the working electrode potential corresponding to the peak moment of the detected electrolysis current, as an example for explanation. In this embodiment, the working electrode 20, the auxiliary electrode 40 and the reference electrode 30 form two loops, namely: a polarization loop for generating an electrolysis current, and a test loop for detecting the potential of the working electrode 20. In this way, the electrolysis current can flow through the working electrode 20 without affecting the detection of the electrolysis current and the working electrode potential by the electronic control component 10 .
[0098] Wherein, the polarization circuit is formed by the auxiliary electrode 40, the working electrode 20 and the polarization power supply device. The polarization power supply in the electric control component 10 outputs an excitation signal to the working electrode 20, so that the working electrode 20 and the auxiliary electrode 40 electrolyze the solution, and generate an electrolysis current on the working electrode 20 for detection by the device for detecting the current. The reference electrode 30, the working electrode 20 and the signal processing device form a detection circuit. In this embodiment, the reference electrode 30 does not participate in polarization, so no polarization current flows, only an extremely small detection current, and the potential of the reference electrode 30 will not change its own potential due to the electrolysis current. In this way, during the detection process, when the signal processing device uses the potential of the reference electrode 30 as a reference potential to obtain the potential of the working electrode 20, the potential of the reference electrode 30 is used as a reference signal. It is beneficial to improve the detection accuracy of the working electrode potential and further improve the accuracy of the bacteria concentration detection pile device.
[0099] In one embodiment of the present invention, referring to Figure 1 and Figure 2 The electronic control component 10 includes a power supply component for outputting an excitation signal, a current detection circuit for detecting the electrolysis current of the working electrode 20, a voltage detection circuit for detecting the potential of the working electrode, and a controller.
[0100] The power supply assembly (i.e. the polarization power supply device) may include a controlled terminal, a first power supply terminal and a second power supply terminal, wherein the first power supply terminal is connected to the working electrode 20 and the second power supply terminal is connected to the auxiliary electrode 40 to form a polarization loop.
[0101] The controlled end of the power supply component is electrically connected to the controller, and the power supply component can be preset with multiple types of excitation signals corresponding to different pulse voltammetry methods. The controller outputs a corresponding control signal to the power supply component according to the pulse voltammetry method actually selected, and the power supply component then outputs a corresponding excitation signal to the polarization circuit according to the control signal received by the controlled end.
[0102] The current detection circuit can be a dedicated current detection chip or a current detection circuit built with discrete components, which is not limited here.
[0103] The controller may be a microprocessor. The microprocessor stores a corresponding relationship curve between the working electrode potential and the bacterial concentration corresponding to the peak moment of the electrolysis current. In this embodiment, the microcontroller includes an excitation signal control terminal for outputting a control signal, a signal receiving terminal for receiving a detection signal of the electrolysis current output by a current detection circuit, and a voltage receiving terminal for receiving the working electrode potential output by a voltage detection circuit.
[0104] The controller obtains the bacterial concentration in the container 50 by inputting the corresponding relationship curve between the working electrode potential and the bacterial concentration corresponding to the peak moment of the electrolysis current output by the current detection circuit.
[0105] Reference Figure 3 and Figure 4 In one embodiment, the excitation signal output by the electronic control component 10 includes a first voltage signal and a second voltage signal output in sequence according to a preset cycle. In multiple consecutive cycles, the amplitude of the first voltage signal in the current cycle increases step by step relative to the amplitude of the first voltage signal in the previous cycle, and the amplitude of the second voltage signal in the current cycle increases step by step relative to the amplitude of the second voltage signal in the previous cycle. The second voltage signal in each cycle is greater than the first voltage signal.
[0106] In other words, the excitation signal output by the electronic control component 10 of the present application is a differential pulse signal.
[0107] In this embodiment, the acquisition of the electrolysis current can be performed by sampling the electrolysis current after the first preset time when the pulse signal appears, so as to improve the accuracy of the electrolysis current detection. The details are as follows:
[0108] Furthermore, the amplitude of the first voltage signal in each cycle remains unchanged or increases linearly according to a preset slope; the amplitude of the second voltage signal in each cycle remains unchanged or increases linearly according to a preset slope.
[0109] Further, refer to Figure 3 , Figure 3is a waveform of an excitation signal, the ordinate is the amplitude of the excitation signal, and the abscissa is time. The excitation signal may be the first excitation signal A1: a signal obtained by superimposing a DC voltage whose amplitude increases linearly with time and a pulse voltage with a constant amplitude. Figure 4 , Figure 4 is another waveform of the excitation signal, the ordinate is the amplitude of the excitation signal, the abscissa is time, and the excitation signal can be the second excitation signal A2: a signal obtained by superimposing a step potential and a pulse voltage with a constant amplitude. Of course, the excitation signal is not limited to the above two signals.
[0110] In one embodiment, the electric control component is used to determine that the bacterial concentration is 1CUF / ml to 10 when the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.270 volts and less than -0.245 volts. 2 CUF / ml between;
[0111] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.285 volts and less than -0.270 volts, the bacterial concentration is determined to be 10 2 CUF / m to 10 8 CUF / m between;
[0112] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.367 volts and less than -0.285 volts, the bacterial concentration is determined to be 10 8 CUF / m to 10 10 CUF / m.
[0113] This embodiment divides the working electrode potential into three potential intervals, each of which corresponds to a bacterial concentration interval. After determining the potential of the working electrode, the bacterial concentration interval can be determined, and the bacterial concentration detection is replaced by the bacterial concentration interval detection, so that the algorithm calculation amount of this embodiment is greatly reduced. The speed of bacterial concentration detection is further improved.
[0114] Reference Figure 1 In one embodiment, the working electrode 20 includes a shell 21 and a metal wire 22, and the shell 21 is provided with a receiving cavity and a through hole 211 connected to the receiving cavity; the metal wire 22 is arranged in the receiving cavity and is electrically connected to the electronic control component 10. In this embodiment, the shell 21 can be made of glass or other materials. It is not limited here. As long as it does not react or react violently with the solution in the container 50, and can protect the metal wire 22, so that the metal wire 22 will not be broken due to factors such as the shaking of the working electrode 20 and the shaking of the container 50, it is sufficient.
[0115] Further, one end of the metal wire 22 is flush with the through hole 211 of the housing 21 or extends a preset length from the through hole 211. It should be noted that the size of the contact area between the metal wire 22 (working electrode 20) and the solution determines the current density of the metal wire 22. The smaller the contact area between the metal wire 22 and the solution in the container 50, the greater the current density of the metal wire 22, and the easier it is to detect the electrolytic current. The larger the contact area between the metal wire 22 and the solution in the container 50, the smaller the current density of the metal wire 22, and the more difficult it is to detect the electrolytic current. In the present embodiment, one end of the metal wire 22 is flush with the through hole 211 of the housing 21 so that the contact area between the metal wire 22 and the solution in the container 50 is only the cross-sectional area of the metal wire 22. The contact area between the metal wire 22 and the solution in the container 50 is effectively reduced, the current density is increased, the electrolytic current is more easily detected by the current detection circuit, and the detection accuracy and detection range of the electrolytic current are improved. At the same time, it can prevent the metal wire 22 from extending out of the housing 21 and causing the very thin metal wire 22 to break due to factors such as shaking.
[0116] Further, the diameter of the cross section of the metal wire 22 is less than or equal to 500 microns. The smaller the diameter of the cross section of the metal wire 22, the smaller the area of the cross section of the metal wire 22, the smaller the contact area between the metal wire 22 and the solution in the container 50, the greater the current density, and the greater the detection accuracy and range of the electrolytic current. Therefore, in this embodiment, the diameter of the cross section of the metal wire 22 is set to be less than or equal to 500 microns, so that solutions with lower concentrations can be detected, thereby increasing the detection range of bacterial concentrations.
[0117] Reference Figure 7 In one embodiment, the bacteria concentration detection component further includes a display module, which is electrically connected to the electronic control component 10 and displays the bacteria concentration in the container 50 under the control of the electronic control component 10 .
[0118] In this embodiment, the display module can be an LED indicator light group (composed of multiple LED lights, lighting different numbers of LED lights to indicate different bacterial concentrations. For example, the LED indicator light includes 5 LED lights, and the bacterial concentration is divided into five intervals. When the detected bacterial concentration falls in the second interval, one LED light is lit, and when the detected bacterial concentration falls in the first interval, two LED lights are lit, and so on), a dot matrix screen, a digital display screen, etc., so as to reduce the volume and cost of the bacterial concentration detection component. Of course, the display module can also be other large display screens to better display the bacterial concentration.
[0119] Reference Figure 7The present invention further proposes a bacteria concentration detection device, which includes a container 50, a gas delivery device 60, a liquid delivery device 70 and the above-mentioned bacteria concentration detection component.
[0120] The gas delivery device 60 is connected to the container 50 and is used to discharge a first preset volume of the gas to be tested into the container 50; the liquid delivery device 70 is connected to the container 50 and is used to discharge a second preset volume of liquid into the container 50; the working electrode 20 and the auxiliary electrode 40 of the bacteria concentration detection component are arranged in the container 50; the electronic control component 10 of the bacteria concentration detection component is also used to determine the bacteria concentration of the gas to be tested according to the bacteria concentration of the liquid in the container 50, the first preset volume and the second preset volume.
[0121] In this embodiment, the container 50 can be any type of device that can hold liquid. The bacteria concentration detection device can include a main controller that controls the operation of the gas delivery device 60 and the infusion device 70, respectively. Of course, the gas delivery device 60 and the infusion device 70 can also be electrically connected to the electric control component 10 of the bacteria concentration detection component, and the electric control component 10 in the bacteria concentration detection component controls the gas delivery device 60 and the infusion device 70. This embodiment is described by taking the example that the gas delivery device 60 and the infusion device 70 are electrically connected to the electric control component 10 of the bacteria concentration detection component, and the electric control component 10 in the bacteria concentration detection component controls the gas delivery device 60 and the infusion device 70,
[0122] The first end of the gas delivery device 60 can be connected to the container 50 storing the gas to be tested. It can also be directly exposed to the air, using the air as the gas to be tested, and the second end of the gas delivery device 60 is connected to the container 50. The gas delivery device 60 can be electrically connected to the electronic control component 10 of the bacteria concentration detection component, and the electronic control component 10 of the bacteria concentration detection component controls it to start working / stop working. The gas delivery device 60 is also provided with a gas flow meter, which is electrically connected to the electronic control component 10 of the bacteria concentration detection component, so that the electronic control component 10 can determine the volume of the gas to be tested discharged into the container 50, and control the gas delivery device 60 to stop working when the volume of the gas to be tested discharged into the container 50 reaches the first preset volume. Of course, the gas delivery device 60 can also preset the gas to be tested to be discharged in the first preset volume each time. And when receiving the trigger signal output by the electronic control component 10, it works once and stops working after the gas to be tested of the first preset volume is discharged.
[0123] The liquid can be pure water or other known liquids. In order to facilitate bacteria detection, this embodiment takes pure water as an example. Therefore, the bacterial content can be determined by simply detecting the bacterial concentration after the pure water and the gas to be tested are mixed. In combination with the volume of the gas to be tested, the bacterial concentration of the gas to be tested can be determined. The controlled end of the infusion device 70 can also be electrically connected to the electronic control component 10 of the bacteria concentration detection component, and the electronic control component 10 of the bacteria concentration detection component controls it to start working / stop working. The infusion device 70 is also provided with a liquid flow meter, which is electrically connected to the electronic control component 10 of the bacteria concentration detection component, so that the electronic control component 10 can determine the volume of the liquid discharged into the container 50, and control the infusion device 70 to stop working when the volume of the liquid discharged into the container 50 reaches the second preset volume. Of course, the infusion device 70 can also preset the liquid discharged into the second preset volume each time. And when receiving the trigger signal output by the electronic control component 10, it works once and stops working after discharging the second preset volume of liquid.
[0124] In this embodiment, a gas delivery device 60 and a liquid delivery device 70 are provided to mix a first preset volume of the gas to be tested and a second preset volume of the liquid to form a mixed solution. The bacterial concentration of the mixed solution is then tested by a bacteria concentration detection component. The bacterial concentration of the mixed solution is obtained. The electronic control component 10 of the bacteria concentration detection component determines the bacterial content based on the bacterial concentration of the mixed solution in the container 50 and the volume of the mitigating solution (the second preset volume). The bacterial concentration of the gas to be tested is then determined based on the gas to be tested, the bacterial content and the volume of the gas to be tested (the first preset volume), thereby realizing the detection of the bacterial concentration of the gas.
[0125] Reference Figure 7 In one embodiment, the gas delivery device 60 includes a gas delivery pipe 61 and an air pump 62, wherein one end of the gas delivery pipe 61 is used to connect the gas to be tested, and the other end is connected to the container 50; the air pump 62 is arranged on the gas delivery pipe 61 and is electrically connected to the electronic control component 10, and the air pump 62 is used to deliver the gas to be tested to the container 50 and control the volume of the delivered gas to be tested.
[0126] In this embodiment, the gas supply pipe 61 may include: an air inlet pipe and an air outlet pipe, wherein one end of the air inlet pipe is used to connect the gas to be tested, and the other end of the air inlet pipe is connected to the air inlet end of the air pump 62, one end of the air outlet pipe is connected to the air outlet end of the air pump 62, and the other end of the air outlet pipe is connected to the container 50.
[0127] The electronic control component 10 controls the air pump 62 to work, outputs the gas to be tested from the gas inlet end to the gas outlet end, and outputs it into the container 50. And when it is determined according to the gas flow meter that the volume of the gas to be tested discharged into the container 50 reaches the first preset volume, the air pump 62 is controlled to stop working.
[0128] Reference Figure 7 In one embodiment, the infusion device 70 includes a storage tank 73, an infusion tube 71 and an infusion pump 72, wherein the storage tank 73 is used to store the liquid; one end of the infusion tube 71 is connected to the storage tank 73, and the other end is connected to the container 50; the infusion pump 72 is arranged on the infusion tube 71 and is electrically connected to the electronic control component 10, and is used to control the volume of the solution delivered from the storage tank 73 to the container 50.
[0129] The storage tank 73 can be isolated from the outside, so that the liquid in the storage tank 73 will not come into contact with air or other interferences and be contaminated, thereby affecting the test accuracy.
[0130] The infusion pump 72 is provided with a liquid flow meter, which is electrically connected to the electronic control component 10 and outputs the detected liquid flow rate to the electronic control component 10 in real time. When the electronic control component 10 determines that the liquid in the container 50 reaches the second preset volume, the infusion pump 72 is controlled to stop working.
[0131] Reference Figure 7 In one embodiment, the bacteria concentration detection device further comprises:
[0132] The liquid discharge device is used to discharge the liquid in the container 50.
[0133] The liquid discharge device may include a liquid discharge pipe and a liquid discharge pump disposed on the liquid discharge pipe, the liquid discharge pump being connected to the electronic control component 10 and operating under the control of the electronic control component 10 to pump out the liquid in the container 50 through the liquid discharge pipe to perform the next bacterial concentration detection, so that the bacterial concentration detection device of this embodiment can be recycled.
[0134] Further, the container 50 is provided with a liquid discharge port;
[0135] The liquid discharge device is a liquid discharge valve, which is disposed at the liquid discharge port and is used to control the liquid discharge port to discharge the liquid in the container 50 when it is opened. The liquid discharge valve is electrically connected to the electronic control component 10, and after determining the bacterial concentration of the liquid in the container 50, the electronic control component 10 controls the liquid discharge valve to open, so as to control the liquid discharge port to discharge the liquid in the container 50, so as to perform the next bacterial concentration detection.
[0136] Furthermore, the bacteria concentration detection device may further include a wastewater storage tank 73, which is connected to the discharge pipe in the discharge device. When the discharge device is working, the waste liquid is stored. Furthermore, the wastewater storage tank 73 may be detachable, so that after each time the waste liquid is fully stored, the wastewater storage tank 73 can be removed and the waste liquid can be poured out. In some embodiments, the wastewater storage tank 73 may further include a waste liquid discharge pipe and a waste liquid discharge valve, and the waste liquid discharge valve is used to discharge the waste liquid in the wastewater storage tank 73 through the waste liquid discharge pipe when triggered.
[0137] In one embodiment, the bacteria concentration detection device may further include a cleaning device, which may be connected to the storage tank 73 or an external pure water access port. The cleaning device is electrically connected to the electronic control component 10. The electronic control component 10 may control the cleaning device to discharge pure water into the container 50 before each bacteria concentration detection. Then, the drain valve is controlled to open to discharge the liquid in the container 50 and clean the container 50. This prevents the residual liquid from the previous bacteria concentration detection from affecting the result of this bacteria concentration detection.
[0138] In one embodiment, the bacteria concentration detection device can be periodically actively triggered, that is, the bacteria concentration detection device works periodically to detect the bacteria concentration of the gas to be detected. In another embodiment, the bacteria concentration detection device can be passively triggered by an input unit, and after passive triggering, the bacteria concentration of the gas to be detected is detected.
[0139] In order to better illustrate the principle of the present invention, refer to Figures 1 to 7 , combined with the above embodiments, the workflow of the present invention is explained.
[0140] After the bacteria concentration detection device is triggered, the electronic control component 10 in the bacteria detection component of the bacteria concentration detection device controls the infusion device 70 to work, discharges the liquid into the container 50, and controls the infusion device 70 to stop working after confirming that the volume of the discharged liquid reaches the second preset volume through the liquid flow meter (at this time, the liquid should be submerged in the second end of the gas delivery device 60, so that the gas to be tested and the liquid can be fully mixed, and the liquid should be submerged in the working electrode 20, the auxiliary electrode 40 and the reference electrode 30). After the infusion is completed, the electronic control component 10 controls the gas delivery device 60 to work, discharge the gas to be tested into the container 50, and fully mix it with the liquid. After confirming that the volume of the gas to be tested reaches the first preset volume through the gas flow meter, the gas delivery device 60 is controlled to stop working. At this time, the mixed solution in the container 50 is the solution to be tested.
[0141] The electric control component 10 outputs an excitation signal to the working electrode 20, so that the working electrode 20 and the auxiliary electrode 40 form a polarization loop, and detects the electrolysis current flowing through the working electrode 20 and the potential of the working electrode, and obtains the potential of the working electrode at the peak moment of the electrolysis current, and determines the bacterial concentration through the relationship curve between the working electrode potential and the bacterial concentration corresponding to the peak moment of the electrolysis current stored by the electric control component 10. Control the display module to display the bacterial concentration. At the same time, control the discharge device to work and discharge the solution in the container 50. And wait for the next trigger to start the next bacterial concentration detection.
[0142] Reference Figure 8 The present invention also provides a method for detecting bacterial concentration, which comprises the following steps:
[0143] S100, discharging a second preset volume of liquid into the container;
[0144] The container can be closed or open. The liquid can be pure water or other liquids. This can be achieved through an infusion pump, an infusion tube and a liquid flow meter. One end of the infusion tube is connected to a storage tank for storing liquid or is directly connected to a water pipe and connected to tap water; the other end of the infusion tube is connected to the container, and the infusion pump controls the infusion tube to discharge the liquid into the container, and the liquid volume discharged is calculated by the liquid flow meter. When the second preset volume is reached, the infusion pump is controlled to stop working.
[0145] S200, discharging a first preset volume of air into the container so that the air and the liquid form a mixed solution;
[0146] This can be achieved through an air pump, an air pipe and a gas flow meter. One end of the air pipe is exposed to the air, and the other end of the air pipe is connected to the container and submerged in the liquid. The air pump controls the air pipe to discharge air into the liquid in the container, and the volume of the discharged air is calculated by the gas flow meter. When the first preset volume is reached, the air pump is controlled to stop working.
[0147] S300, outputting an excitation signal to the working electrode to electrolyze the mixed solution of air and liquid in the container;
[0148] The excitation signal can be sent out by the power supply assembly and act on the working electrode and the auxiliary electrode immersed in the mixed solution to stimulate the working electrode, the auxiliary electrode and the solution to be tested to form a polarization loop and generate an electrolysis current.
[0149] S400, obtaining the electrolysis current of the working electrode;
[0150] The electrolysis current can be obtained by a current detection circuit electrically connected to the working electrode, such as a current Hall sensor.
[0151] S500, obtaining the potential of the working electrode corresponding to the peak moment of the electrolysis current according to the electrolysis current;
[0152] The potential of the working electrode can be obtained by using the potential of the reference electrode or the auxiliary electrode as a reference potential through a voltage detection circuit.
[0153] S600, obtaining the bacterial concentration of the mixed solution according to the potential of the working electrode corresponding to the peak moment of the electrolysis current;
[0154] S700: Determine the bacterial concentration of the air according to the bacterial concentration of the mixed solution, the first preset volume, and the second preset volume.
[0155] The microprocessor can be connected to the above-mentioned infusion pump, liquid flow meter, air pump, gas flow meter, power supply assembly, current detection circuit, voltage detection circuit, working electrode, reference electrode, and auxiliary electrode respectively. The microprocessor stores a program for detecting the concentration of bacteria in the air, and implements the method for detecting the concentration of bacteria when executing the program for detecting the concentration of bacteria. The microprocessor can establish / store a relationship curve or table between the working electrode potential corresponding to the peak moment of the current and the bacterial concentration, and bring the working electrode potential corresponding to the peak moment of the electrolysis current into the relationship curve or table to estimate the bacterial concentration or concentration range. The microprocessor can calculate the bacterial content based on the bacterial concentration and the second preset volume of the liquid. Then divide the bacterial content by the first preset gas phase of the air. The bacterial concentration in the air is calculated.
[0156] In this embodiment, when the corresponding data of the working electrode potential corresponding to the current peak moment cannot be found in the table, two adjacent data in the table can be obtained, and the interval formed by the two data includes the working electrode potential corresponding to the current peak moment, and then according to which of the two data has a smaller difference between the working electrode potential corresponding to the current peak moment, it is determined that the bacterial concentration corresponding to the data with the smaller difference is the bacterial concentration we need to obtain.
[0157] In one embodiment, the step of obtaining the bacterial concentration of the mixed solution according to the potential of the working electrode corresponding to the peak moment of the electrolysis current is specifically:
[0158] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.270 volts and less than -0.245 volts, the bacterial concentration of the mixed solution is determined to be 1 CUF / ml to 10 2 CUF / ml between;
[0159] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.285 volts and less than -0.270 volts, the bacterial concentration of the mixed solution is determined to be 10 2 CUF / m to 10 8 CUF / m between;
[0160] When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.367 volts and less than -0.285 volts, the bacterial concentration of the mixed solution is determined to be 10 8 CUF / m to 10 10 CUF / m.
[0161] The potential of the working electrode corresponding to the peak moment of the collected electrolysis current can be compared with multiple potential intervals by a microprocessor to determine the potential interval to which the potential of the working electrode belongs, and the concentration interval can be determined according to the mapping relationship between the potential interval and the concentration interval. In practical applications, corresponding operations can be performed directly according to the concentration interval. For example, when the concentration interval described in the bacterial concentration is large, it is displayed that the bacterial concentration exceeds the standard.
[0162] In one embodiment, the diameter of the working electrode is less than 500 microns.
[0163] The smaller the diameter of the working electrode, the greater the current density, the more convenient it is to detect the electrolytic current, and the higher the detection accuracy. This embodiment improves the detection accuracy of the electrolytic current, and ultimately improves the detection accuracy of the air bacteria concentration.
[0164] The present invention also provides an electrical device, the electrical device comprising the above-mentioned bacteria concentration detection component; or bacteria concentration detection device. The specific structure of the bacteria concentration detection component or the bacteria concentration detection device refers to the above-mentioned embodiment. Since the electrical device adopts all the technical solutions of all the above-mentioned embodiments, it at least has all the beneficial effects brought by the technical solutions of the above-mentioned embodiments, which will not be described one by one here.
[0165] The container 50 of the bacteria concentration detection device can be an existing container 50 in the electrical equipment, that is, a container 50 is reused with other functional modules. Of course, it can also be a separate container 50 for bacteria concentration detection.
[0166] The storage tank 73 of the bacteria concentration detection device may be an existing container 50 in the electrical device. For example, when the electrical device is a humidifier, the storage tank 73 of the bacteria concentration detection device may be a water storage tank in the humidifier.
[0167] The bacteria concentration detection device / bacteria concentration detection component can also be connected to the main controller of the electrical equipment, and output the detected air bacteria concentration to the main controller. The main controller of the electrical equipment then determines the next step according to the bacteria concentration.
[0168] For example, when the electrical device is an air purifier, the gas to be tested is air, and when the bacteria concentration detection device determines that the air bacteria concentration exceeds the standard, the intensity of air purification is increased. When the air bacteria concentration is determined to be less than a preset value, the intensity of air purification is reduced.
[0169] When the electrical device is a humidifier / pet waterer, etc., the solution in the container 50 is the liquid in the humidifier / pet waterer. When the bacteria concentration detection component determines that the bacteria concentration of the liquid in the humidifier / pet waterer exceeds a preset value, the device stops working.
[0170] In one embodiment, the electrical device is an air conditioner, an air purifier, a humidifier or a sterilizer.
[0171] The above descriptions are only optional embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.
Claims
1. A bacteria concentration detection device, characterized in that: The bacteria concentration detection device comprises: container; A gas delivery device, connected to the container, and used to discharge a first preset volume of the gas to be tested into the container; an infusion device, connected to the container, and used to discharge a second preset volume of liquid into the container; and a bacteria concentration detection component, the bacteria concentration detection component comprising: A working electrode, configured to be disposed in the container; an auxiliary electrode, configured to be disposed in the container; and An electric control component, electrically connected to the working electrode and the auxiliary electrode, respectively, the electric control component is used to output an excitation signal to the working electrode so that the working electrode and the auxiliary electrode electrolyze the solution in the container; and is used to detect the electrolysis current and the working electrode potential of the working electrode, and determine the bacterial concentration of the solution in the container according to the working electrode potential corresponding to the peak moment of the detected electrolysis current, the working electrode and the auxiliary electrode of the bacteria concentration detection component are arranged in the container; The electronic control component of the bacteria concentration detection component is also used to determine the bacteria concentration of the gas to be tested according to the bacteria concentration of the liquid in the container, the first preset volume and the second preset volume.
2. The bacteria concentration detection device according to claim 1, characterized in that: The electronic control assembly comprises: a power supply component, electrically connected to the working electrode and the auxiliary electrode respectively, and configured to output an excitation signal to the working electrode so that the working electrode and the auxiliary electrode electrolyze the solution in the container; A current detection circuit, used for detecting the electrolysis current of the working electrode; a voltage detection circuit, used for detecting a working electrode potential of the working electrode; and A controller is connected to the power supply assembly and the current detection circuit respectively, and is used to determine the bacterial concentration of the solution in the container according to the working electrode potential corresponding to the peak moment of the detected electrolysis current.
3. The bacteria concentration detection device according to claim 1, characterized in that: The bacteria concentration detection component also includes: A reference electrode is electrically connected to the electronic control component. The reference electrode is used to form a test loop with the working electrode and provide a voltage reference signal for the electronic control component.
4. The bacteria concentration detection device according to any one of claims 1 to 3, characterized in that: The excitation signal output by the electronic control component includes a first voltage signal and a second voltage signal output in sequence according to a preset cycle. In multiple consecutive cycles, the amplitude of the first voltage signal in the current cycle increases step by step compared with the amplitude of the first voltage signal in the previous cycle, and the amplitude of the second voltage signal in the current cycle increases step by step compared with the amplitude of the second voltage signal in the previous cycle. The second voltage signal in each cycle is greater than the first voltage signal.
5. The bacteria concentration detection device according to claim 4, characterized in that: The amplitude of the first voltage signal in each cycle remains unchanged or increases linearly according to a preset slope; The amplitude of the second voltage signal in each cycle remains unchanged or increases linearly according to a preset slope.
6. The bacteria concentration detection device according to claim 1, characterized in that: The electric control component is used to determine that the bacterial concentration is 1CUF / ml to 10 when the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.270 volts and less than -0.245 volts. 2 CUF / ml between; When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.285 volts and less than -0.270 volts, the bacterial concentration is determined to be 10 2 CUF / m to 10 8 CUF / m between; When the working electrode potential corresponding to the peak moment of the electrolysis current is greater than or equal to -0.367 volts and less than -0.285 volts, the bacterial concentration is determined to be 10 8 CUF / m to 10 10 CUF / m.
7. The bacteria concentration detection device according to any one of claims 1 to 3, characterized in that: The working electrode comprises: A housing, wherein a housing cavity and a through hole communicating with the housing cavity are provided inside the housing; A metal wire is electrically connected to the electric control component, and the metal wire is arranged in the accommodating cavity.
8. The bacteria concentration detection device according to claim 7, characterized in that: One end of the metal wire is flush with the through hole of the housing or extends out of the through hole by a preset length.
9. The bacteria concentration detection device according to claim 7, characterized in that: The diameter of the cross section of the metal wire is less than or equal to 500 micrometers.
10. The bacteria concentration detection device according to any one of claims 1 to 3, characterized in that: The bacteria concentration detection component also includes: A display module, electrically connected to the electronic control component; The electronic control component is also used to control the display module to display the detected bacteria concentration.
11. The bacteria concentration detection device according to claim 1, characterized in that: The gas delivery device comprises: A gas delivery pipe, one end of which is used to receive the gas to be tested, and the other end of which is connected to the container; The air pump is arranged on the gas delivery pipe and is electrically connected to the electronic control component, and is used for delivering the gas to be tested to the container and controlling the volume of the delivered gas to be tested.
12. The bacteria concentration detection device according to claim 1, characterized in that: The infusion device comprises: A storage tank for storing the liquid; an infusion tube, one end of which is connected to the storage tank and the other end of which is connected to the container; The infusion pump is arranged on the infusion tube and is electrically connected to the electronic control component, and is used for controlling the volume of the solution delivered from the storage tank to the container.
13. The bacteria concentration detection device according to claim 1, characterized in that: The bacteria concentration detection device also includes: The liquid discharge device is used to discharge the liquid in the container.
14. The bacteria concentration detection device according to claim 13, characterized in that: The container is provided with a liquid discharge port; The liquid discharge device is a liquid discharge valve, which is arranged at the liquid discharge port and is used to control the liquid discharge port to discharge the liquid in the container when it is opened.
Citation Information
Patent Citations
Electrochemical biological sensor
CN2556643Y