Cell culture device

Abstract

The present application proposes a cell culture device, including a stirring component, a culture dish and a base, wherein the stirring component is arranged on the base, a cavity is arranged between the stirring component and the base, and the culture dish is placed on the base in the cavity; the stirring component is used to stir the culture solution and make the culture solution produce a certain flow state to avoid the precipitation or floating of nutrients in the culture solution, and the stirring component includes: a support cover, a shock-absorbing cover, a connector and a stirring mechanism. The cell culture device proposed in the embodiment of the present application can fully stir the culture solution in the shock-absorbing cover, and can form a flow state from the culture dish through the liquid inlet hole to the shock-absorbing cover, and from the shock-absorbing cover through the reflux hole to the culture dish. Therefore, the cell culture device of the embodiment of the present application stirs the culture solution to make the nutrients in the culture solution evenly distributed, and makes the culture solution form a flow state, avoids the precipitation or floating of nutrients in the culture solution, and does not cause the culture dish to vibrate, which is beneficial to the growth of cultured cells.

Description

Technical Field

[0001] The present application relates to the field of cell culture technology, and in particular to a cell culture device. Background Art

[0002] In the related art, cells are generally cultured in a cell culture dish, wherein the cell culture dish contains culture medium. However, during the process of culturing cells, nutrients in the culture medium may precipitate or float, thereby causing uneven distribution of nutrients required by the cells.

[0003] Currently, cell culture dishes are vibrated to prevent nutrients in the culture medium from settling or floating. A common vibration method is to place a vibration table at the bottom of the culture dish. This vibration method will affect the growth of cells cultured in the culture dish (for example, the vibration of the culture dish will affect the adhesion ability of the cells, thereby affecting the adhesion, extension, migration and other behaviors of the cells, which is not conducive to the growth of the cultured cells). Summary of the invention

[0004] The present application aims to solve one of the technical problems in the above-mentioned technology at least to some extent.

[0005] To this end, one purpose of the present application is to propose a cell culture device, which stirs the culture medium to evenly distribute the nutrients in the culture medium and makes the culture medium flow, thereby avoiding the precipitation or floating of the nutrients in the culture medium and preventing the culture dish from vibrating, thereby facilitating the growth of cultured cells.

[0006] To achieve the above objectives, one embodiment of the present application provides a cell culture device, including: a stirring component, a culture dish and a base.

[0007] Wherein, the stirring component is arranged on the base, a cavity is arranged between the stirring component and the base, and the culture dish is placed in the cavity;

[0008] The stirring assembly is used to stir the culture liquid in the culture dish so that the culture liquid is in a flowing state. The stirring assembly comprises: a support cover, a shock-absorbing cover, a connecting piece and a stirring mechanism, wherein the support cover is a hollow structure with an open end, the open end of the support cover is detachably connected to the base, and a connecting piece is provided on the top of the inner side of the support cover; the shock-absorbing cover is provided on the connecting piece, the shock-absorbing cover extends into the culture dish, a reflux hole is provided in the middle position of the shock-absorbing cover, and a liquid inlet hole is provided at the bottom end of the shock-absorbing cover;

[0009] The stirring mechanism is arranged on the connecting member, and the stirring mechanism is located in the shock-absorbing cover; the stirring mechanism includes a driving motor arranged on the connecting member, the driving motor is provided with a stirring rod, the stirring rod is provided with a stirring blade, one end of the stirring blade is connected to the end of the stirring rod away from the driving motor, and the stirring blade spirals upward around the stirring rod.

[0010] According to the cell culture device of the embodiment of the present application, the culture liquid in the shock-absorbing cover can be fully stirred, and the culture liquid can be made to flow from the culture dish through the liquid inlet hole to the shock-absorbing cover, and from the shock-absorbing cover through the reflux hole to the culture dish, thereby avoiding the precipitation or floating of nutrients in the culture liquid and preventing the culture dish from vibrating, which is beneficial to the growth of cultured cells.

[0011] In addition, the cell culture device proposed in the above embodiment of the present application may also have the following additional technical features:

[0012] In one embodiment of the present application, a card slot is arranged around the opening end of the support cover.

[0013] In one embodiment of the present application, a reflow wall is provided on the shock-absorbing cover, the upper portion of the reflow wall is connected to the shock-absorbing cover at a preset angle and extends to the outside of the shock-absorbing cover, the lower portion of the reflow wall extends inwardly and is connected to the shock-absorbing cover, and the reflow hole is provided at the connection between the lower portion of the reflow wall and the shock-absorbing cover.

[0014] In one embodiment of the present application, a flow limiting plate extending upward is provided at the connection between the lower portion of the reflow wall and the shock-absorbing cover.

[0015] In one embodiment of the present application, a sliding sleeve is provided on the inner top of the support cover, and the connecting member can be slidably installed in the sliding sleeve; a sliding groove is provided on the sliding sleeve, and a sliding block is provided on the connecting member, and the sliding block can be slidably installed in the sliding groove.

[0016] In one embodiment of the present application, a sliding push rod is provided at the top of the connecting member, one end of the sliding push rod is connected to the connecting member, and the other end of the sliding push rod extends upward and penetrates the support cover.

[0017] In one embodiment of the present application, a guide groove is provided on the stirring blade, and the guide groove extends to both ends of the stirring blade.

[0018] In one embodiment of the present application, a constant temperature heating plate is provided in the base.

[0019] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which:

[0021] Figure 1 is a schematic structural diagram of a cell culture device according to one embodiment of the present application;

[0022] Figure 2 is a schematic structural diagram of a cell culture device according to another embodiment of the present application;

[0023] Figure 3 is a schematic structural diagram of a cell culture device according to another embodiment of the present application;

[0024] Figure 4 is a schematic structural diagram of a cell culture device according to another embodiment of the present application;

[0025] Figure 5 Schematic diagram of the cross-sectional structure of a stirring blade of a cell culture device according to one embodiment of the present application.

[0026] Figure numerals: 100, stirring assembly, 110, support cover, 111, slot, 120, shock-absorbing cover, 121, reflux wall, 122, reflux hole, 123, liquid inlet hole, 124, current limiting plate, 130, connecting piece, 131, sliding sleeve, 132, sliding groove, 133, sliding block, 134, sliding push rod, 140, driving motor, 141, stirring rod, 142, stirring blade, 200, culture dish, 300, base, 310, constant temperature heating plate, 1421, guide groove. DETAILED DESCRIPTION

[0027] Embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

[0028] The cell culture device according to the embodiment of the present application will be described below with reference to the accompanying drawings.

[0029] like Figure 1 and Figure 2 As shown, the cell culture device according to the embodiment of the present application includes a stirring assembly 100 , a culture dish 200 and a base 300 .

[0030] The stirring assembly 100 is disposed on the base 300, a cavity is disposed between the stirring assembly 100 and the base 300, and the culture dish 200 is placed on the base 300 in the cavity. The stirring assembly 100 is used to stir the culture solution to keep the culture solution in a flowing state, so as to avoid the precipitation or floating of nutrients in the culture solution.

[0031] The stirring assembly 100 includes a support cover 110, a shock-absorbing cover 120, a connecting piece 130 and a stirring mechanism 100, wherein the support cover 110 is a hollow structure with one end open, the open end of the support cover 110 is detachably connected to the base 300, a cavity structure is formed between the support cover 110 and the base 300, and a connecting piece 130 is provided on the inner top of the support cover 110.

[0032] Specifically, if Figure 1 As shown, the open end of the support cover 110 described in this embodiment is provided with a card slot 111 that matches the size of the base 300. When the support cover 110 is mounted on the base 300, the card slot 111 is stuck at the edge of the base 300, and the edge of the support cover 110 is located below the upper surface of the base 300. At this time, the support cover 110 cooperates with the base 300 to form a relatively closed cavity structure, and the base 300 in the cavity can be used to place the culture dish 200.

[0033] A shock-absorbing cover 120 is disposed on the connecting member 130 . The shock-absorbing cover 120 extends into the culture dish 200 . A reflux hole 122 is disposed in the middle of the shock-absorbing cover 120 , and a liquid inlet hole 123 is disposed at the bottom of the shock-absorbing cover 120 .

[0034] Specifically, if Figure 2 and Figure 4 As shown, the shock-absorbing cover 120 described in this embodiment is provided with a return wall 121, the upper part of the return wall 121 is connected to the shock-absorbing cover 120 at a preset angle and extends to the outside of the shock-absorbing cover 120, the lower part of the return wall 121 extends inwardly and is connected to the shock-absorbing cover 120, and the return hole 122 is provided at the connection between the lower part of the return wall 121 and the shock-absorbing cover 120.

[0035] It can be understood that the reflux wall 121 described in this embodiment extends outward and then folds back to form a wall body with a certain slope, which makes it easier for the culture fluid to flow along the reflux holes 122 on the inner wall of the shock-absorbing cover 120 to the outside of the shock-absorbing cover 120 and then flow into the culture dish 200, compared with a vertical downward wall body. This is conducive to the formation of a flowing state of the culture fluid in the cell culture device.

[0036] As a possible scenario, Figure 2As shown, a flow limiting plate 124 extending upward is provided at the connection between the lower part of the reflux wall 121 and the shock-absorbing cover 120 described in this embodiment. The flow limiting plate 124 can limit the culture fluid on the reflux wall 121 from flowing downward, and then allow the culture fluid to flow into the culture dish 200 through the reflux hole 122, which can further improve the flow efficiency of the culture fluid in the cell culture device.

[0037] It should be noted that if Figure 2 As shown, the flow limiting plate 124 described in this embodiment and the lower part of the reflux wall 121 can form a groove around the shock-absorbing cover 120. When the reflux hole 122 cannot meet the reflux demand of the culture fluid, the above groove can temporarily retain the culture fluid to prevent the culture fluid from flowing back to the bottom of the shock-absorbing cover 120.

[0038] A stirring mechanism is provided on the connecting member 130, and the stirring mechanism is located in the shock-absorbing cover 120. The stirring mechanism includes a driving motor 140 provided on the connecting member 130, a stirring rod 141 is provided on the driving motor 140, and a stirring blade 142 is provided on the stirring rod 141. One end of the stirring blade 142 is connected to the end of the stirring rod 141 away from the driving motor 140, and the stirring blade 142 spirals upward around the stirring rod 141.

[0039] Specifically, the stirring mechanism described in this embodiment can stir the culture liquid in the shock-absorbing cover 120. During the stirring process, the stirring blade 142 can throw the culture liquid onto the inner wall of the shock-absorbing cover 120 due to its special structure. After the culture liquid is thrown onto the inner wall of the shock-absorbing cover 120, it flows downward along the inner wall of the shock-absorbing cover 120. In the process of the culture liquid flowing downward, part of it will flow from the reflux hole 122 to the outside of the shock-absorbing cover 120, and flow along the shock-absorbing cover 120 into the culture dish 200.

[0040] As a possible scenario, Figure 2 and Figure 5 As shown, the stirring blade 142 described in this embodiment is provided with a guide groove 1421, and the guide groove 1421 extends to both ends of the stirring blade 142. When the stirring rod 141 rotates, under the action of inertia, part of the culture liquid will flow upward along the guide groove 1421, and after flowing to the top of the stirring blade 142, it will be thrown out by the rotating stirring blade 142.

[0041] It should be noted that if Figure 2 As shown, the position of the upper end of the stirring blade described in this embodiment is higher than the return wall 121 and the flow restriction plate 124.

[0042] The cell culture device proposed in the embodiment of the present application, during specific operation, the culture dish 200 containing the culture solution is placed on the base 300, and the stirring assembly 100 is installed on the base 300. At this time, the shock-absorbing cover 120 of the stirring mechanism is immersed in the culture solution in the culture dish 200, and the culture solution enters the shock-absorbing cover 120 from the liquid inlet 123 at the bottom of the shock-absorbing cover 120. The stirring mechanism in the shock-absorbing cover 120 starts to stir the culture solution. During the stirring process, the stirring blade 142 can throw the culture solution onto the inner wall of the shock-absorbing cover 120 due to its special structure. After being thrown onto the inner wall of the shock-absorbing cover 120, the culture solution flows downward along the inner wall of the shock-absorbing cover 120. During the downward flow of the culture solution, it flows from the reflux hole 122 to the outside of the shock-absorbing cover 120, and flows along the shock-absorbing cover 120 into the culture dish 200.

[0043] In one embodiment of the present application, Figure 2 and Figure 3 As shown, a sliding sleeve 131 is provided on the inner top of the support cover 110, and the connecting member 130 can be slidably installed in the sliding sleeve 131; a sliding groove 132 is provided on the sliding sleeve 131, and a sliding block 133 is provided on the connecting member 130, and the sliding block 133 can be slidably installed in the sliding groove 132.

[0044] Specifically, the connecting member 130 can be slidably installed in the sliding sleeve 131, so that the shock-absorbing cover 120 and the stirring mechanism can be adjusted up and down in the supporting cover 110, so as to be suitable for culture dishes 200 of various heights.

[0045] As a possible scenario, Figure 2 As shown, a sliding push rod 134 is disposed at the top of the connecting member 130, one end of the sliding push rod 134 is connected to the connecting member 130, and the other end of the sliding push rod 134 extends upward and penetrates the support cover 110. The sliding push rod 134 extends to the outside of the support cover 110, and the sliding push rod 134 can be operated from the outside, which is conducive to improving the operating convenience of the cell culture device.

[0046] It is understandable that the side wall of the support cover 110 described in this embodiment can be made of a transparent material, which is conducive to convenient observation of the interior thereof during use.

[0047] In one embodiment of the present application, Figure 2 As shown, a constant temperature heating plate 310 is provided in the base 300 described in this embodiment, which can heat the culture dish 200 and maintain it at a certain temperature.

[0048] In the cell culture device provided in the embodiment of the present application, during specific operation, the culture dish 200 containing the culture solution is placed on the base 300, and the stirring assembly 100 is installed on the base 300. At this time, the shock-absorbing cover 120 of the stirring mechanism is immersed in the culture solution in the culture dish 200, and the culture solution enters the shock-absorbing cover 120 from the liquid inlet 123 at the bottom of the shock-absorbing cover 120. The stirring mechanism in the shock-absorbing cover 120 starts to stir the culture solution.

[0049] During the stirring process, the stirring blade 142, due to its spiral upward structure on the stirring rod 141, can throw the culture medium onto the inner wall of the shock-absorbing cover 120 under the action of inertia. After being thrown onto the inner wall of the shock-absorbing cover 120, the culture medium flows downward along the inner wall of the shock-absorbing cover 120, especially the culture medium thrown onto the reflux wall. During the downward flow process, it will flow from the reflux hole 122 to the outside of the shock-absorbing cover 120, and flow along the shock-absorbing cover 120 into the culture dish 200.

[0050] In addition, the culture dish 200 placed on the base 300 can be kept at a certain temperature under the action of the constant temperature heating plate 310.

[0051] In summary, the cell culture device proposed in the embodiment of the present application can fully stir the culture solution in the shock-absorbing cover 120, and can form a flow state from the culture dish 200 through the liquid inlet hole 123 to the shock-absorbing cover 120, and from the shock-absorbing cover 120 through the reflux hole 122 to the culture dish 200. Therefore, by stirring the culture solution, the nutrients in the culture solution are evenly distributed, and the culture solution is made to flow, so as to avoid the precipitation or floating of the nutrients in the culture solution, and the culture dish will not vibrate, which is beneficial to the growth of cultured cells.

[0052] In the description of this specification, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0053] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0054] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A cell culture device, characterized in that: include: A stirring assembly, a culture dish and a base, wherein: The stirring component is arranged on the base, a cavity is arranged between the stirring component and the base, and the culture dish is placed in the cavity; The stirring assembly is used to stir the culture liquid in the culture dish to make the culture liquid flow. The stirring assembly includes: a support cover, a shock-absorbing cover, a connecting piece and a stirring mechanism, wherein: The support cover is a hollow structure with one end open, the open end of the support cover is detachably connected to the base, and a connecting piece is provided on the inner top of the support cover; The shock-absorbing cover is arranged on the connecting piece, the shock-absorbing cover extends into the culture dish, a reflux hole is arranged in the middle of the shock-absorbing cover, and a liquid inlet hole is arranged at the bottom end of the shock-absorbing cover; The stirring mechanism is arranged on the connecting member, and the stirring mechanism is located in the shock-absorbing cover; The stirring mechanism comprises a driving motor arranged on the connecting member, the driving motor is provided with a stirring rod, the stirring rod is provided with a stirring blade, one end of the stirring blade is connected to the end of the stirring rod away from the driving motor, and the stirring blade spirals upward around the stirring rod; The shock-absorbing cover is provided with a reflux wall, the upper portion of which is connected to the shock-absorbing cover at a preset angle and extends to the outside of the shock-absorbing cover, the lower portion of which extends inwardly and is connected to the shock-absorbing cover, and the reflux hole is provided at the connection between the lower portion of the reflux wall and the shock-absorbing cover; A flow limiting plate extending upward is provided at the connection between the lower part of the return wall and the shock-absorbing cover; The stirring blade is provided with a guide groove, and the guide groove extends to both ends of the stirring blade.

2. The cell culture device according to claim 1, characterized in that: A clamping groove is arranged around the opening end of the support cover.

3. The cell culture device according to claim 1, characterized in that: A sliding sleeve is provided on the inner top of the support cover, and the connecting member can be slidably installed in the sliding sleeve; The sliding sleeve is provided with a sliding groove, the connecting piece is provided with a sliding block, and the sliding block can be slidably installed in the sliding groove.

4. The cell culture device according to claim 3, characterized in that: A sliding push rod is arranged at the top end of the connecting member, one end of the sliding push rod is connected to the connecting member, and the other end of the sliding push rod extends upward and penetrates the supporting cover.

5. The cell culture device according to claim 1, characterized in that: A constant temperature heating plate is arranged in the base.

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