A liquid strain fully automatic fermentation culture system

Abstract

The present invention discloses a liquid strain fully automatic fermentation and cultivation system in the field of liquid strain fermentation and cultivation technology, including a fermentation tank and an air inlet pipe, a reduction motor is fixedly installed on the top of the fermentation tank, the bottom end of the reduction motor is transmission-connected with a stirring shaft located inside the fermentation tank, the bottom end of the outer surface of the stirring shaft is fixedly connected with a No. 2 stirring paddle, a No. 2 connecting groove is provided inside the No. 2 stirring paddle, and a sealing column and a No. 3 spring are movably sleeved on the inner wall of the No. 2 connecting groove. The present invention utilizes the resistance of the culture fluid to the No. 1 stirring paddle to form a compressive force when the No. 1 spring is rotated, and then presses the No. 2 collar, the card block and the moving block downward so that the moving block enters the No. 2 connecting groove, reduces the air passing area of ​​the No. 2 connecting groove, and then increases the air flow rate, so that the sterile air entering the inner cavity of the fermentation tank directly dissolves into the interior of the culture fluid and then reacts rapidly, thereby increasing the reaction rate of the culture fluid.

Description

Technical Field

[0001] The invention relates to the technical field of liquid strain fermentation and cultivation, and in particular to a liquid strain full-automatic fermentation and cultivation system. Background Art

[0002] Compared with solid strain production, liquid strain production reduces many manual processes such as material preparation, ingredient mixing, and mixing, and has the advantages of low cost and short time. Liquid strains form an independent fully automatic fermentation and culture system during fermentation and culture.

[0003] At present, the existing fully automatic fermentation and cultivation systems for liquid strains are mostly based on cylindrical fermentation tanks. The liquid strain materials are stirred by built-in stirring paddles and then fermented. However, some liquid strains require an aerobic environment during fermentation. The existing oxygen supply methods are mostly located on the surface of the liquid material, which greatly reduces the contact area between the liquid strain material and the air, not only causing the pressure in the fermentation tank to be unable to be balanced in time, but also being detrimental to the combination of air and material.

[0004] At the same time, since the speed and torque of the stirring paddle are constant during the stirring process, it is easy for the bacteria in the material to be constantly located in a certain area under the action of centrifugal force, which is not conducive to sufficient reaction after stirring; in addition, the liquid bacteria material will produce additional resistance to the high-speed rotating stirring paddle, which on the one hand increases the energy burden of the motor and transmission mechanism, and at the same time, it is impossible for the fermented liquid bacteria to quickly enter the reaction state. Summary of the invention

[0005] The object of the present invention is to provide a fully automatic fermentation and cultivation system for liquid bacteria, so as to solve the problems of low material-air contact efficiency, low stirring efficiency and energy waste proposed in the above-mentioned background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a fully automatic fermentation and cultivation system for liquid bacteria, comprising a fermentation tank and an air inlet pipe, a reduction motor is fixedly installed on the top of the fermentation tank, the bottom end of the reduction motor is transmission-connected to a stirring shaft located inside the fermentation tank, a No. 2 stirring paddle is fixedly connected to the bottom end of the outer surface of the stirring shaft, a No. 2 connecting groove is provided inside the No. 2 stirring paddle, a sealing column and a No. 3 spring are movably sleeved on the inner wall of the No. 2 connecting groove, an air outlet is provided on the outer surface of the No. 2 stirring paddle, and the outer surface of the No. 2 stirring paddle is fixedly connected to the stirring shaft. The stirring shaft is fixedly connected with an air outlet mechanism, a No. 1 connecting groove is provided inside the stirring shaft, and an air filter cartridge is fixedly sleeved on the inner wall of the No. 1 connecting groove, an air intake mechanism is provided at the bottom end of the stirring shaft, a No. 2 sleeve ring, a No. 1 spring, a No. 1 sleeve ring and a No. 1 stirring paddle are movably sleeved on the outer surface of the stirring shaft, a clamping block is movably clamped on the top of the No. 2 stirring paddle, a sealing groove is provided on the top of the inner wall of the No. 2 stirring paddle, a moving block is fixedly connected to the bottom of the clamping block, and a No. 4 spring is elastically connected between the top of the inner wall of the clamping block and the No. 2 stirring paddle;

[0007] When the system is working, the feed inlet is opened, and an appropriate amount of bacterial culture liquid is poured into the inner cavity of the fermentation tank and the liquid level is made to cover the No. 1 stirring paddle. Then the feed inlet is closed, the air inlet pipe is connected to the air pump externally, and air is introduced into the No. 1 connecting groove through the sealing shaft and the connecting pipe. The reduction motor is started and the stirring shaft and the No. 2 stirring paddle are driven to rotate. The rotating No. 2 stirring paddle stirs and mixes the bacterial culture liquid. Inside the No. 2 connecting groove: the sealing column is moved toward the No. 3 spring by the centrifugal force and the high-pressure airflow from the air pump and compresses the No. 3 spring, so that the sealing column no longer forms a blocking effect on the air outlet. At the same time, the air outlet mechanism also releases the blockage of the air outlet under the action of centrifugal force, so that the sterile air filtered and purified by the air filter cartridge enters the inner cavity of the fermentation tank, thereby being fully mixed with the bacterial culture liquid. The preparation work before fermentation is completed; the rotating No. 2 stirring paddle drives the No. 2 sleeve ring to rotate, and then drives the No. 1 spring to make the lower end of the No. 1 spring rotate around its own axis, so that the No. 1 spring is stressed and drives the No. 1 sleeve ring and the No. 1 stirring paddle to rotate. Since the No. 1 stirring paddle is subjected to the liquid resistance of the culture solution, the speed difference between it and the No. 2 stirring paddle is caused, so that the stirring frequencies of the upper and lower parts of the culture solution in the inner cavity of the fermenter are different. On the one hand, the stirring degree of the culture solution is more uniform. At the same time, the No. 1 spring that is rotated and stressed is equivalent to being compressed, thereby pushing the No. 2 sleeve ring and the block downward, driving the moving block to move downward along the inner wall of the sealing groove, thereby reducing the cross-sectional area of ​​the No. 2 connecting groove, increasing the air passing speed, and improving the contact and reaction efficiency between the culture solution and the sterile air.

[0008] By providing a No. 2 stirring paddle connected to the stirring shaft, the air from the outside of the air inlet pipe enters the No. 2 connecting groove after being aseptically treated by the air filter cartridge. In the process of the reduction motor driving the stirring shaft and the No. 2 stirring paddle to rotate, the air moves in the direction of the No. 3 spring through the centrifugal force generated in the No. 2 connecting groove, thereby releasing the blockage on the inside of the air outlet. Then, the centrifugal force acts on the air outlet mechanism to release the blockage on the outside of the air outlet. At the same time, the rotating No. 2 stirring paddle drives the No. 2 collar and rotates, and the resistance of the culture medium to the No. 1 stirring paddle forms a compressive force when the No. 1 spring is rotated, thereby pressing the No. 2 collar, the card block and the moving block downward so that the moving block enters the No. 2 connecting groove, reducing the air passing area of ​​the No. 2 connecting groove, thereby increasing the air flow rate, so that the sterile air entering the inner cavity of the fermenter is directly dissolved into the interior of the culture medium and then reacts rapidly, thereby increasing the reaction rate of the culture medium.

[0009] By providing a No. 2 sleeve ring and a No. 2 stirring paddle that are movably connected, the rotating No. 2 stirring paddle drives the No. 1 spring to rotate through the No. 2 sleeve ring, and the liquid resistance formed by the culture liquid on the No. 1 stirring paddle forms a speed difference between the No. 1 stirring paddle and the No. 2 stirring paddle. As the degree of force applied to the No. 1 spring by rotation gradually increases, its rotational torque gradually becomes greater than the liquid resistance applied to the No. 1 stirring paddle, and then gradually drives the No. 1 sleeve ring and the No. 1 stirring paddle to rotate at a low speed, forming different stirring frequencies in the upper and lower partitions of the culture liquid, so that the substances in the culture liquid that are constantly distributed due to the centrifugal force will not be concentrated in one area, so that the reaction after stirring is more timely and sufficient.

[0010] As a further solution of the present invention, the No. 2 sleeve ring is fixedly connected to the clamping block, the upper and lower ends of the No. 1 spring are elastically connected to the No. 1 sleeve ring and the No. 2 sleeve ring respectively, the outer surface of the No. 1 sleeve ring is fixedly connected to the No. 1 stirring paddle, and the top of the No. 1 sleeve ring is in limiting contact with a limiting ring fixedly sleeved on the outer surface of the stirring shaft;

[0011] The No. 2 stirring paddle, which is driven by the stirring shaft to rotate at a high speed, drives the No. 1 ring and the No. 1 stirring paddle to rotate through the No. 2 ring and the No. 1 spring. The No. 1 stirring paddle rotates at a low speed between the resistance of the culture medium and the rotational torque from the No. 2 stirring paddle, thereby maintaining the rotational speed of the upper area of ​​the culture medium at a very low level, so that convection is formed in the upper and lower partitions of the culture medium.

[0012] By providing a No. 1 spring with its two ends fixedly connected to the No. 1 sleeve and the No. 2 sleeve respectively, when the stirring shaft drives the No. 2 stirring paddle and the No. 2 sleeve to rotate at high speed, the rotational pressure on the No. 1 spring causes the torsional potential energy of the No. 1 spring to gradually increase, thereby reducing the resistance effect of the culture fluid on the No. 1 stirring paddle and reducing the rotational kinetic energy loss of the stirring shaft. At the same time, the No. 2 stirring paddle rotates and stirs the culture fluid, and the rotational speed of the culture fluid drives the No. 1 stirring paddle to perform auxiliary rotation, thereby reducing the torsional pressure on the stirring shaft and reducing the energy loss from the reduction motor.

[0013] As a further solution of the present invention, the air outlet mechanism includes an air outlet pipe, the outer surface of the air outlet pipe is movably sleeved with a limit plate, a second spring is elastically connected between the inner wall of the limit plate and the outer surface of the air outlet pipe, a sealing plate is fixedly connected to the front side of the limit plate, and the sealing plate is in sealing contact with the opening of the air outlet pipe;

[0014] The limit plate is fixedly connected to the outer surface of the outlet pipe through the No. 2 spring. When the stirring shaft drives the No. 2 stirring paddle to rotate, the centrifugal force drives the limit plate to move outward along the axial direction of the No. 2 stirring paddle, stretching the No. 2 spring, thereby driving the sealing plate to leave the opening of the outlet pipe, allowing sterile air to enter the inner cavity of the fermentation tank. At the same time, when the rotation stops, the elastic potential energy of the No. 2 spring is used to pull the sealing plate back to its original position, and the hydraulic pressure of the culture fluid is used to apply pressure to the sealing plate to seal the opening of the outlet pipe.

[0015] As a further solution of the present invention, the air intake mechanism includes a sealing shaft fixedly installed at the bottom of the fermentation tank, the left side of the sealing shaft is fixedly connected with an air intake pipe, the internal sealing sleeve of the sealing shaft is connected with a connecting pipe, and the top of the connecting pipe is fixedly connected to the stirring shaft; the air intake pipe can be connected to an external air pump to provide an air source, the connecting pipe is fixedly connected to the stirring shaft, and the connecting pipe is driven to rotate during the rotation of the stirring shaft, so that the connecting pipe sealing sleeve is connected to the inside of the sealing shaft and does not affect the air intake.

[0016] As a further solution of the present invention, a feed port is fixedly connected to the right side of the top of the fermenter, an observation port is fixedly installed on the front of the fermenter, and a discharge port is fixedly installed on the right side of the bottom of the fermenter; the feed port is responsible for feeding, and is fixed and sealed by a flange, with high reliability, and the observation port is built-in with transparent high-temperature resistant glass, which can allow staff to observe the culture liquid in the inner cavity of the fermenter.

[0017] As a further solution of the present invention, the number of the second stirring paddles is two and they are respectively distributed on the left and right sides of the outer surface of the stirring shaft, and the second connecting groove is connected to the first connecting groove;

[0018] The No. 2 stirring paddle is symmetrically distributed on the outer surface of the stirring shaft. The outside air from the air inlet pipe can enter the No. 2 connecting groove through the No. 1 connecting groove and complete the sterilization operation in the air filter cartridge. The rectangular distribution of the cross-section of the No. 2 stirring paddle can increase the degree of stirring of the culture solution and improve the reaction efficiency.

[0019] As a further solution of the present invention, the sealing column is elastically supported on the inner wall of the No. 2 connecting groove by a No. 3 spring, and the outer surface of the sealing column is in sealing contact with the air outlet;

[0020] The No. 3 spring applies pressure to the sealing column so that its outer surface is sealed at the opening of the air outlet. When the stirring shaft stops rotating, the air outlet is sealed. On the one hand, it can prevent outside air from entering the inner cavity of the fermentation tank and destroying the sterile environment inside the fermentation tank. At the same time, it can prevent the culture medium from entering the No. 2 connecting tank.

[0021] As a further solution of the present invention, the longitudinal cross-section of the card block is an inverted "U" shape, the bottom of the card block is adapted to be snap-connected to the top of the No. 2 stirring paddle, and the two sides of the bottom of the card block are fixedly connected to the moving block; the inverted "U" shape design of the card block enables it to move up and down while being snap-connected and rotating synchronously, thereby completing the driving function of the moving block.

[0022] As a further solution of the present invention, the distance between the No. 2 stirring paddle and the inner wall of the fermentation tank is equal to the distance between the No. 1 stirring paddle and the inner wall of the fermentation tank; the stirring degrees of the No. 1 stirring paddle and the No. 2 stirring paddle need to be kept the same, so as to keep the stirring frequency of the culture medium synchronized.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The present invention is provided with a No. 2 stirring paddle connected with the stirring shaft, so that the air from the outside of the air inlet pipe enters the No. 2 connecting groove after the aseptic treatment of the air filter cartridge. In the process of the reduction motor driving the stirring shaft and the No. 2 stirring paddle to rotate, the air moves in the direction of the No. 3 spring through the centrifugal force generated in the No. 2 connecting groove, thereby releasing the blockage of the inner side of the air outlet, and then the centrifugal force acts on the air outlet mechanism to release the blockage of the outer side of the air outlet. At the same time, the No. 2 sleeve ring is driven and rotated by the rotating No. 2 stirring paddle, and the resistance of the culture medium to the No. 1 stirring paddle is used to form a compression force when the No. 1 spring is rotated, and then the No. 2 sleeve ring, the card block and the moving block are pressed downward so that the moving block enters the No. 2 connecting groove, reducing the air passing area of ​​the No. 2 connecting groove, thereby increasing the air flow rate, so that the sterile air entering the inner cavity of the fermenter is directly dissolved in the interior of the culture medium and then reacts rapidly, thereby increasing the reaction rate of the culture medium.

[0025] 2. The present invention is provided with a No. 2 sleeve ring and a No. 2 stirring paddle that are movably connected, so that the rotating No. 2 stirring paddle drives the No. 1 spring to rotate through the No. 2 sleeve ring, and utilizes the liquid resistance formed by the culture fluid on the No. 1 stirring paddle to form a speed difference between the No. 1 stirring paddle and the No. 2 stirring paddle. As the degree of force applied to the No. 1 spring by rotation gradually increases, its rotational torque gradually becomes greater than the liquid resistance applied to the No. 1 stirring paddle, and then gradually drives the No. 1 sleeve ring and the No. 1 stirring paddle to rotate at a low speed, forming different stirring frequencies in the upper and lower partitions of the culture fluid, so that the substances in the culture fluid that are constantly distributed due to the centrifugal force will not be concentrated in one area, so that the reaction after stirring is more timely and sufficient.

[0026] 3. The present invention provides a No. 1 spring so that its two ends are fixedly connected to the No. 1 ring and the No. 2 ring respectively. When the stirring shaft drives the No. 2 stirring paddle and the No. 2 ring to rotate at high speed, the rotational pressure on the No. 1 spring is applied, so that the torsional potential energy of the No. 1 spring is gradually increased. On the one hand, the resistance effect of the culture solution on the No. 1 stirring paddle is reduced, and the rotational kinetic energy loss of the stirring shaft is reduced. At the same time, the No. 2 stirring paddle rotates and stirs the low culture solution, and the No. 1 stirring paddle is driven by the rotation speed of the culture solution to perform auxiliary rotation, thereby reducing the torsional pressure on the stirring shaft and reducing the energy loss from the reduction motor. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 A schematic diagram of the internal structure of the overall structure of the present invention from the front;

[0029] Figure 3 The present invention Figure 2 A magnified schematic diagram of the structure at center A;

[0030] Figure 4 A partial front cutaway schematic diagram of the overall structure of the present invention;

[0031] Figure 5 The present invention Figure 4 A magnified schematic diagram of the structure at B in the middle;

[0032] Figure 6 A schematic top view of the stirring shaft and the second stirring paddle of the present invention;

[0033] Figure 7 An exploded schematic diagram of the stirring shaft, No. 1 collar, No. 1 stirring paddle, No. 1 spring, No. 2 collar, a clamping block, No. 2 stirring paddle, a sealing groove and an air outlet mechanism of the present invention;

[0034] Figure 8 It is a longitudinal cross-sectional schematic diagram of the second stirring paddle and the moving block of the present invention;

[0035] Fig. 9An exploded schematic diagram of the second sleeve ring, the clamping block, the second stirring paddle, the air outlet mechanism and the fourth spring of the present invention.

[0036] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0037] 1. Fermentation tank; 101. Feed inlet; 102. Observation port; 103. Discharge port; 2. Speed ​​reducer; 3. Sealing shaft; 4. Inlet pipe; 5. Stirring shaft; 6. No. 1 collar; 7. No. 1 stirring paddle; 8. No. 1 spring; 9. No. 2 collar; 10. Block; 11. No. 2 stirring paddle; 12. Sealing groove; 13. Exhaust mechanism; 131. Limiting plate; 132. Sealing plate; 133. No. 2 spring; 134. Exhaust pipe; 14. Connecting pipe; 15. No. 1 connecting groove; 16. Air filter cartridge; 17. No. 2 connecting groove; 18. Sealing column; 19. No. 3 spring; 20. Moving block; 21. No. 4 spring; 22. Exhaust port. DETAILED DESCRIPTION

[0038] See also Figure 1-9 The present invention provides a technical solution: a fully automatic fermentation and cultivation system for liquid strains, comprising a fermentation tank 1 and an air inlet pipe 4, a reduction motor 2 is fixedly installed on the top of the fermentation tank 1, the bottom end of the reduction motor 2 is transmission-connected with a stirring shaft 5 located inside the fermentation tank 1, a second stirring paddle 11 is fixedly connected to the bottom end of the outer surface of the stirring shaft 5, a second connecting groove 17 is provided inside the second stirring paddle 11, a sealing column 18 and a third spring 19 are movably sleeved on the inner wall of the second connecting groove 17, an air outlet 22 is provided on the outer surface of the second stirring paddle 11, and an air outlet mechanism is fixedly connected to the outer surface of the second stirring paddle 11 13. A No. 1 connecting groove 15 is provided inside the stirring shaft 5, and an air filter cartridge 16 is fixedly sleeved on the inner wall of the No. 1 connecting groove 15. An air intake mechanism is provided at the bottom end of the stirring shaft 5. A No. 2 sleeve ring 9, a No. 1 spring 8, a No. 1 sleeve ring 6 and a No. 1 stirring paddle 7 located at the top of the No. 2 stirring paddle 11 are movably sleeved on the outer surface of the stirring shaft 5. A clamping block 10 is movably clamped on the top of the No. 2 stirring paddle 11. A sealing groove 12 is provided on the top of the inner wall of the No. 2 stirring paddle 11. A moving block 20 is fixedly connected to the bottom of the clamping block 10. A No. 4 spring 21 is elastically connected between the top of the inner wall of the clamping block 10 and the No. 2 stirring paddle 11;

[0039] When the system is working, the feed inlet 101 is opened, and a proper amount of bacterial culture liquid is poured into the inner cavity of the fermentation tank 1 so that the liquid level is above the No. 1 stirring paddle 7, and then the feed inlet 101 is closed, the air inlet pipe 4 is connected to the air pump externally, and air is introduced into the No. 1 connecting groove 15 through the sealing shaft 3 and the connecting pipe 14, and the reduction motor 2 is started to drive the stirring shaft 5 and the No. 2 stirring paddle 11 to rotate, and the rotating No. 2 stirring paddle 11 stirs and mixes the bacterial culture liquid, and the sealing column 18 inside the No. 2 connecting groove 17 is moved toward the No. 3 spring 19 by the centrifugal force and the high-pressure airflow from the air pump and compresses the No. 3 spring 19, so that the sealing column 18 no longer forms a blocking effect on the air outlet 22, and at the same time, the air outlet mechanism 13 also releases the blocking of the air outlet 22 under the action of the centrifugal force, so that the sterile air filtered and purified by the air filter cartridge 16 enters the inner cavity of the fermentation tank 1, thereby fully mixing with the bacteria. The stirring paddle No. 11 in rotation drives the No. 2 collar 9 to rotate, and then drives the No. 1 spring 8 to make the lower end of the No. 1 spring 8 rotate around its own axis, so that the No. 1 spring 8 is stressed and drives the No. 1 collar 6 and the No. 1 stirring paddle 7 to rotate. Since the No. 1 stirring paddle 7 is subjected to the liquid resistance of the culture solution and causes the speed difference between the No. 2 stirring paddle 11, the stirring frequencies of the upper and lower parts of the culture solution in the inner cavity of the fermenter 1 are different. On the one hand, the stirring degree of the culture solution is more uniform. At the same time, the No. 1 spring 8 subjected to the force of rotation is equivalent to being compressed, thereby pushing the No. 2 collar 9 and the block 10 downward, and driving the moving block 20 to move downward along the inner wall of the sealing groove 12, thereby reducing the cross-sectional area of ​​the No. 2 connecting groove 17, increasing the air passing speed, and improving the contact and reaction efficiency between the culture solution and the sterile air.

[0040] The second stirring paddle 11 is connected to the stirring shaft 5, so that the air from the outside of the air inlet pipe 4 enters the second connecting groove 17 after being aseptically treated by the air filter cartridge 16. When the reduction motor 2 drives the stirring shaft 5 and the second stirring paddle 11 to rotate, the centrifugal force generated in the second connecting groove 17 moves in the direction of the third spring 19, thereby releasing the blockage of the inner side of the air outlet 22. Then, the centrifugal force acts on the air outlet mechanism 13 to release the blockage of the outer side of the air outlet 22. At the same time, the rotation The moving No. 2 stirring paddle 11 drives the No. 2 collar 9 and the No. 1 stirring paddle 7 to rotate, and utilizes the resistance effect of the culture fluid on the No. 1 stirring paddle 7 to form a compressive force when the No. 1 spring 8 is rotated, thereby pressing the No. 2 collar 9, the block 10 and the moving block 20 downward so that the moving block 20 enters the No. 2 connecting groove 17, reducing the air passing area of ​​the No. 2 connecting groove 17, thereby increasing the air flow rate, so that the sterile air entering the inner cavity of the fermenter 1 is directly dissolved into the interior of the culture fluid and then reacts rapidly, thereby increasing the reaction rate of the culture fluid.

[0041] By providing the No. 2 sleeve ring 9 and the No. 2 stirring paddle 11 with movable engagement, the rotating No. 2 stirring paddle 11 drives the No. 1 spring 8 to rotate through the No. 2 sleeve ring 9, and the liquid resistance formed by the culture fluid on the No. 1 stirring paddle 7 forms a speed difference between the No. 1 stirring paddle 7 and the No. 2 stirring paddle 11. As the degree of force applied to the No. 1 spring 8 by rotation gradually increases, its rotational torque gradually becomes greater than the liquid resistance applied to the No. 1 stirring paddle 7, and then gradually drives the No. 1 sleeve ring 6 and the No. 1 stirring paddle 7 to rotate at a low speed, forming different stirring frequencies in the upper and lower partitions of the culture fluid, so that the substances in the culture fluid that are constantly distributed due to the centrifugal force will not be concentrated in one area, so that the reaction after stirring is more timely and sufficient.

[0042] Among them, the No. 2 sleeve ring 9 is fixedly connected to the clamping block 10, the upper and lower ends of the No. 1 spring 8 are elastically connected to the No. 1 sleeve ring 6 and the No. 2 sleeve ring 9 respectively, the outer surface of the No. 1 sleeve ring 6 is fixedly connected to the No. 1 stirring paddle 7, and the top of the No. 1 sleeve ring 6 is in limited contact with the limiting ring fixedly sleeved on the outer surface of the stirring shaft 5;

[0043] The No. 2 stirring paddle 11 driven by the stirring shaft 5 to rotate at a high speed drives the No. 1 ring 6 and the No. 1 stirring paddle 7 to rotate through the No. 2 ring 9 and the No. 1 spring 8. The No. 1 stirring paddle 7 rotates at a low speed between the resistance of the culture solution and the rotational torque from the No. 2 stirring paddle 11, thereby maintaining the rotation speed of the upper area of ​​the culture solution at a very low level, so that convection is formed in the upper and lower partitions of the culture solution.

[0044] By providing a No. 1 spring 8 with its two ends fixedly connected to the No. 1 collar 6 and the No. 2 collar 9 respectively, when the stirring shaft 5 drives the No. 2 stirring paddle 11 and the No. 2 collar 9 to rotate at high speed, the rotational pressure on the No. 1 spring 8 is applied, so that the torsional potential energy of the No. 1 spring 8 is gradually increased, on the one hand, the resistance effect of the culture solution on the No. 1 stirring paddle 7 is reduced, and the rotational kinetic energy loss of the stirring shaft 5 is reduced. At the same time, the No. 2 stirring paddle 11 is rotated and stirred with the low culture solution, and the No. 1 stirring paddle 7 is driven by the rotation speed of the culture solution to perform auxiliary rotation, thereby reducing the torsional pressure on the stirring shaft 5 and reducing the energy loss from the reduction motor 2.

[0045] The air outlet mechanism 13 includes an air outlet pipe 134, the outer surface of the air outlet pipe 134 is movably sleeved with a limit plate 131, a second spring 133 is elastically connected between the inner wall of the limit plate 131 and the outer surface of the air outlet pipe 134, a sealing plate 132 is fixedly connected to the front of the limit plate 131, and the sealing plate 132 is in sealing contact with the opening of the air outlet pipe 134;

[0046] The limit plate 131 is fixedly connected to the outer surface of the outlet pipe 134 through the No. 2 spring 133. When the stirring shaft 5 drives the No. 2 stirring paddle 11 to rotate, the centrifugal force drives the limit plate 131 to move outward along the axial direction of the No. 2 stirring paddle 11, stretching the No. 2 spring 133, thereby driving the sealing plate 132 to leave the opening of the outlet pipe 134, allowing sterile air to enter the inner cavity of the fermentation tank 1. At the same time, when the rotation stops, the elastic potential energy of the No. 2 spring 133 is used to pull the sealing plate 132 back to its original position, and the hydraulic pressure of the culture fluid is used to pressurize the sealing plate 132 to seal the opening of the outlet pipe 134.

[0047] Among them, the air intake mechanism includes a sealing shaft 3 fixedly installed at the bottom of the fermentation tank 1, an air intake pipe 4 is fixedly connected to the left side of the sealing shaft 3, the inner sealing sleeve of the sealing shaft 3 is connected with a connecting pipe 14, and the top of the connecting pipe 14 is fixedly connected to the stirring shaft 5; the air intake pipe 4 can be connected to an external air pump to provide an air source, and the connecting pipe 14 is fixedly connected to the stirring shaft 5. When the stirring shaft 5 rotates, the connecting pipe 14 is driven to rotate, so that the connecting pipe 14 is sealed and sleeved with the inside of the sealing shaft 3 without affecting the air intake.

[0048] Among them, a feed port 101 is fixedly connected to the right side of the top of the fermenter 1, an observation port 102 is fixedly installed on the front of the fermenter 1, and a discharge port 103 is fixedly installed on the right side of the bottom of the fermenter 1; the feed port 101 is responsible for feeding, and it is fixed and sealed by a flange, with high reliability, and the observation port 102 is built-in with transparent high-temperature resistant glass, which can allow staff to observe the culture liquid in the inner cavity of the fermenter 1.

[0049] There are two second stirring paddles 11, which are respectively distributed on the left and right sides of the outer surface of the stirring shaft 5, and the second connecting groove 17 is connected to the first connecting groove 15;

[0050] The No. 2 stirring paddle 11 is distributed on the outer surface of the stirring shaft 5 in a symmetrical form. The outside air from the air inlet pipe 4 can enter the No. 2 connecting groove 17 through the No. 1 connecting groove 15, and complete the sterilization operation in the air filter cartridge 16. The rectangular distribution of the cross-section of the No. 2 stirring paddle 11 can increase the degree of stirring of the culture solution and improve the reaction efficiency.

[0051] The sealing column 18 is elastically supported on the inner wall of the No. 2 connecting groove 17 by the No. 3 spring 19, and the outer surface of the sealing column 18 is in sealing contact with the air outlet 22;

[0052] The No. 3 spring 19 applies pressure to the sealing column 18 so that its outer surface is sealed at the opening of the air outlet 22. When the stirring shaft 5 stops rotating, the air outlet 22 is sealed. On the one hand, it can prevent external air from entering the inner cavity of the fermentation tank 1 and destroying the sterile environment inside the fermentation tank 1. At the same time, it can prevent the culture medium from entering the No. 2 connecting groove 17.

[0053] Among them, the longitudinal cross-section of the block 10 is an inverted "U" shape, the bottom of the block 10 is adapted to be snap-connected to the top of the second stirring paddle 11, and the two sides of the bottom of the block 10 are fixedly connected to the moving block 20; the inverted "U" shape design of the block 10 enables it to move up and down while being snap-connected and rotating synchronously, thereby completing the driving function of the moving block 20.

[0054] Among them, the distance between the No. 2 stirring paddle 11 and the inner wall of the fermentation tank 1 is equal to the distance between the No. 1 stirring paddle 7 and the inner wall of the fermentation tank 1; the stirring degrees of the No. 1 stirring paddle 7 and the No. 2 stirring paddle 11 need to be kept the same, so as to keep the stirring frequency of the culture medium synchronized.

[0055] Working principle:

[0056] When the system is working, the feed inlet 101 is opened, and a proper amount of bacterial culture liquid is poured into the inner cavity of the fermentation tank 1 so that the liquid level is above the No. 1 stirring paddle 7, and then the feed inlet 101 is closed, the air inlet pipe 4 is connected to the air pump and air is introduced into the No. 1 connecting groove 15 through the sealing shaft 3 and the connecting pipe 14, the reduction motor 2 is started and the stirring shaft 5 and the No. 2 stirring paddle 11 are driven to rotate, and the rotating No. 2 stirring paddle 11 stirs and mixes the bacterial culture liquid, and the inside of the No. 2 connecting groove 17: the sealing column 18 is moved toward the No. 3 spring 19 by the centrifugal force and the high-pressure airflow from the air pump and compresses the No. 3 spring 19, so that the sealing column 18 no longer forms a blocking effect on the air outlet 22, and at the same time, the air outlet mechanism 13 also releases the blocking of the air outlet 22 under the action of the centrifugal force①, so that the sterile air filtered and purified by the air filter cartridge 16 enters the inner cavity of the fermentation tank 1, thereby fully mixing with the sterile air. The culture medium of the bacterial strain is mixed to complete the preparation work before fermentation; the rotating No. 2 stirring paddle 11 drives the No. 2 collar 9 to rotate, and then drives the No. 1 spring 8 to make the lower end of the No. 1 spring 8 rotate around its own axis, so that the No. 1 spring 8 is stressed and drives the No. 1 collar 6 and the No. 1 stirring paddle 7 to rotate. Since the No. 1 stirring paddle 7 is subjected to the liquid resistance of the culture medium, the speed difference between it and the No. 2 stirring paddle 11 is caused, so that the stirring frequencies of the upper and lower parts of the culture medium in the inner cavity of the fermenter 1 are different. On the one hand, the stirring degree of the culture medium is more uniform. At the same time, the No. 1 spring 8 that is rotated and stressed is equivalent to being compressed, thereby pushing the No. 2 collar 9 and the block 10 downward, and driving the moving block 20 to move downward along the inner wall of the sealing groove 12, thereby reducing the cross-sectional area of ​​the No. 2 connecting groove 17, increasing the air passing speed, and improving the contact and reaction efficiency between the culture medium and the sterile air.

Claims

1. A fully automatic fermentation and cultivation system for liquid bacteria, comprising a fermentation tank (1) and an air inlet pipe (4), wherein a reduction motor (2) is fixedly mounted on the top of the fermentation tank (1), and the bottom end of the reduction motor (2) is drivingly connected to a stirring shaft (5) located inside the fermentation tank (1), characterized in that: The bottom end of the outer surface of the stirring shaft (5) is fixedly connected to a No. 2 stirring paddle (11), a No. 2 connecting groove (17) is provided inside the No. 2 stirring paddle (11), a sealing column (18) and a No. 3 spring (19) are movably sleeved on the inner wall of the No. 2 connecting groove (17), an air outlet (22) is provided on the outer surface of the No. 2 stirring paddle (11), an air outlet mechanism (13) is fixedly connected to the outer surface of the No. 2 stirring paddle (11), a No. 1 connecting groove (15) is provided inside the stirring shaft (5), and an air filter cartridge (16) is fixedly sleeved on the inner wall of the No. 1 connecting groove (15), An air intake mechanism is provided at the bottom end of the stirring shaft (5); a No. 2 sleeve ring (9), a No. 1 spring (8), a No. 1 sleeve ring (6) and a No. 1 stirring paddle (7) are movably sleeved on the outer surface of the stirring shaft (5); a clamping block (10) is movably clamped on the top of the No. 2 stirring paddle (11); a sealing groove (12) is provided on the top of the inner wall of the No. 2 stirring paddle (11); a moving block (20) is fixedly connected to the bottom of the clamping block (10); and a No. 4 spring (21) is elastically connected between the top of the inner wall of the clamping block (10) and the No. 2 stirring paddle (11); The No. 2 sleeve ring (9) and the clamping block (10) are fixedly connected, the upper and lower ends of the No. 1 spring (8) are elastically connected to the No. 1 sleeve ring (6) and the No. 2 sleeve ring (9) respectively, the outer surface of the No. 1 sleeve ring (6) is fixedly connected to the No. 1 stirring paddle (7), and the top of the No. 1 sleeve ring (6) is in limiting contact with a limiting ring fixedly sleeved on the outer surface of the stirring shaft (5); The sealing column (18) is elastically supported on the inner wall of the No. 2 connecting groove (17) via a No. 3 spring (19), and the outer surface of the sealing column (18) is in sealing contact with the air outlet (22).

2. A liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The air outlet mechanism (13) comprises an air outlet pipe (134), the outer surface of the air outlet pipe (134) is movably sleeved with a limit plate (131), a No. 2 spring (133) is elastically connected between the inner wall of the limit plate (131) and the outer surface of the air outlet pipe (134), a sealing plate (132) is fixedly connected to the front side of the limit plate (131), and the sealing plate (132) is in sealing contact with the opening of the air outlet pipe (134).

3. The liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The air intake mechanism comprises a sealing shaft (3) fixedly mounted on the bottom of the fermentation tank (1); an air intake pipe (4) is fixedly connected to the left side of the sealing shaft (3); a connecting pipe (14) is connected to the inner sealing sleeve of the sealing shaft (3); and the top end of the connecting pipe (14) is fixedly connected to the stirring shaft (5).

4. The liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The right side of the top of the fermentation tank (1) is also fixedly connected with a feed port (101), the front of the fermentation tank (1) is fixedly installed with an observation port (102), and the right side of the bottom of the fermentation tank (1) is fixedly installed with a discharge port (103).

5. The liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The number of the second stirring paddles (11) is two and they are respectively distributed on the left and right sides of the outer surface of the stirring shaft (5), and the second connecting groove (17) is connected to the first connecting groove (15).

6. The liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The longitudinal cross-section of the card block (10) is an inverted "U" shape, the bottom of the card block (10) is adapted to be snap-connected to the top of the second stirring paddle (11), and the two sides of the bottom of the card block (10) are fixedly connected to the moving block (20).

7. The liquid strain fully automatic fermentation culture system according to claim 1, characterized in that: The distance between the second stirring paddle (11) and the inner wall of the fermentation tank (1) is equal to the distance between the first stirring paddle (7) and the inner wall of the fermentation tank (1).

Images