Cell membrane jet perforator device and method, macromolecular substance transmembrane transport system

Abstract

The embodiment of the present application belongs to the field of macromolecular substance transmembrane transport, and particularly relates to a cell membrane jet flow perforation device and method and a macromolecular substance transmembrane conduction system. The cell membrane jet flow perforation device comprises a cell introduction flow cavity, a cell capture cavity and a jet flow device. The cell introduction flow cavity has an inlet and an outlet. The cell capture cavity is in communication with the cell introduction flow cavity, and is used for capturing cells entering the cell capture cavity from the inlet. The jet flow device provides jet flow to the cells in the cell capture cavity, so that the cell membranes of the cells in the cell capture cavity form at least one micropore. Compared with the prior art, the perforation device of the present embodiment can perforate the cell membrane in a mechanical manner. When the cells are subjected to the shearing force of the flow field, a plurality of transient micropores can be formed on the cell membranes. In the case of minimizing the damage to the cells, high-throughput direct transmembrane transport of macromolecular substances is realized.

Description

TECHNICAL FIELD

[0001] The embodiment of the present application belongs to the field of macromolecular substance transmembrane transport, and particularly relates to a cell membrane jet perforation device and method and a macromolecular substance transmembrane conduction system. BACKGROUND

[0002] Cell reprogramming is a key technology in the field of cell biology, and has a wide range of applications in regenerative medicine, disease models and drug screening. Previous studies have shown that induced pluripotent stem cells can be obtained by ectopic expression of specific transcription factors to reprogram somatic cells. Gene delivery technology can also induce cell reprogramming by introducing target genes into cells.

[0003] There are mainly biological methods, physical methods and mechanical methods for gene transcellular delivery. Biological methods include gene transformation, conjugation and transduction. Physical methods include microinjection, particle bombardment, gene gun, electroporation and optical methods. At present, the widely used delivery method is viral transduction. Viral vectors can ensure stable expression of transgenes in induced pluripotent stem cells, but they pose a risk of tumorigenesis and other biological safety issues. Some scholars have therefore devoted themselves to the research of using proteins to induce pluripotent stem cells. One of the common methods for introducing proteins into cells is electroporation. This method produces an electroporation effect, which may randomly cause cell membrane rupture and lead to cell death, and the transfection range is also limited by the direction of the electric field. Therefore, the existing biological, physical methods may cause permanent damage or even death to cells, or pose a biological safety risk due to viral transfection and gene insertion, or be limited in their wide application due to their applicability to only a few types or quantities of cells.

[0004] However, the use of mechanical methods can cause cell membrane deformation and substance transmembrane transport, and has certain advantages in biological safety. By precisely controlling the stress on the cell membrane, it is possible to achieve efficient substance transport and ensure cell survival rate. Massachusetts Institute of Technology has proposed a cell extrusion technology, which drives cells through a contraction channel that is 30%-80% smaller than their diameter, causing them to be extruded and deformed, and prompting the opening of force-sensitive channels on the cell membrane. Current research has confirmed that this method can be applied to many types of cells and has wide applicability. However, the inventors have found that there are two factors restricting the development of the current cell extrusion technology. First, the diameter of the compression channel for transmembrane transport of substances in cells is about several microns, which puts high requirements on microfabrication technology. Second, cells of the same type vary in size, and cells that are slightly larger may block the channel, while cells that are slightly smaller may not deform enough, resulting in reduced transport efficiency.

[0005] Therefore, how to realize the transmembrane transport of macromolecular substances by using a better mechanical method is a problem to be solved at present. SUMMARY

[0006] In order to solve the above problems or at least partially solve the above technical problems, in some embodiments of the present application, a cell membrane jet perforation device and method, and a macromolecular substance transmembrane conduction system are designed, so that the cell can realize the transmembrane transport of macromolecular substances under the action of external force.

[0007] In order to achieve the above purpose, some embodiments of the present application provide a cell membrane jet perforation device, comprising:

[0008] A cell introduction flow cavity is provided, which has an inlet, an outlet away from the inlet, and a delivery hole arranged between the inlet and the outlet;

[0009] A cell capture cavity is provided, which is in communication with the cell introduction flow cavity, and is used for capturing cells entering the cell introduction flow cavity from the inlet;

[0010] A jet device is provided, which provides a jet to the cell capture cavity, so that the cell membrane of the cell sucked into the cell capture cavity forms at least one micropore.

[0011] In addition, the embodiments of the present application design a macromolecular substance transmembrane conduction system, which comprises the cell membrane jet perforation device as described above.

[0012] In addition, the embodiments of the present application design a cell membrane jet perforation method, which uses the cell membrane jet perforation device as described above to perforate the cell membrane of the cell, and the cell membrane perforation method comprises the following steps:

[0013] The cell entering the cell introduction flow cavity from the inlet is captured by the cell capture cavity;

[0014] The jet device is controlled to provide a jet of a preset intensity to the cell capture cavity within a preset time length, so that the cell membrane of the cell sucked into the cell capture cavity forms at least one micropore;

[0015] The cell which has completed the perforation and the macromolecular substance conduction in the cell capture cavity is obtained from the outlet by using negative pressure.

[0016] The embodiment of the present application has the following advantages over the prior art. The cell membrane jet flow perforating device comprises a cell introduction flow cavity, a cell capturing cavity and a jet flow device. In practical application, based on the variable cross-section pipe vortex principle, the cells entering the cell introduction flow cavity from the inlet can enter the cell capturing cavity along the vortex of the flow field, and thus be captured by the cell capturing cavity. Then, the jet flow device provides jet flow to the cell capturing cavity, and the cells in the capturing cavity are subjected to the flow field shear force generated by the jet flow impact, resulting in violent deformation, and one or more micropores are formed on the cell membrane. At this time, the macromolecular substances in the solution enter the cells through the micropores, and after the jet flow is completed, the cell membrane micropores heal by themselves, and the cells in the cell capturing cavity can be obtained from the outlet by using negative pressure. It can be seen that when the flow field shear force of the device is controlled within a proper range, one or more micropores are formed on the cell membrane, and the transmembrane transport of macromolecular substances is realized with the least cell damage.

[0017] In addition, the bottom of the cell capturing cavity is provided with a jet flow inlet; the jet flow device comprises:

[0018] a jet flow generating cavity connected with the jet flow inlet; one side of the jet flow generating cavity relative to the jet flow inlet is not closed, forming an opening, and the opening is coaxially arranged with the jet flow inlet;

[0019] a vibrating component arranged on the side of the jet flow generating cavity relative to the jet flow inlet, and closing the opening;

[0020] a driving component connected with the vibrating component; the driving component is used to drive the vibrating component to vibrate along the axis direction of the jet flow inlet.

[0021] In addition, one side of the jet flow generating cavity relative to the jet flow inlet protrudes along the axis direction of the jet flow inlet, forming a jet flow pipe connected with the jet flow inlet.

[0022] In addition, one end of the jet flow pipe away from the jet flow inlet is in the shape of an acute angle.

[0023] In addition, the opening, the jet flow pipe and the jet flow inlet are coaxially arranged.

[0024] In addition, the vibrating component is a vibrating membrane;

[0025] Alternatively, the vibrating component is a vibrating block.

[0026] In addition, the driving component is a piezoelectric driving component;

[0027] Alternatively, the driving component is a driving component with mechanical properties.

[0028] In addition, the cell capturing cavity and the jet device constitute a cell jet perforation module.

[0029] The cell jet perforation module is arranged in the cell introduction flow cavity.

[0030] In addition, the cell capturing cavities are arranged in sequence on the same side of the cell introduction flow cavity.

[0031] Alternatively, the cell capturing cavities are arranged in pairs on opposite sides of the cell introduction flow cavity. BRIEF DESCRIPTION OF DRAWINGS

[0032] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the description of the embodiments or the prior art will be briefly introduced. Obviously, the drawings in the following description are only used to illustrate some embodiments of the present application, and for those skilled in the art, other technical features, connection relationships and even method steps not mentioned in the drawings can be obtained without creative labor on the basis of these drawings.

[0033] Figure 1 Structure diagram of the cell membrane jet perforation device of the first embodiment of the present application;

[0034] Figure 2 When the inner surface of the jet pipe is a conical surface during assembly of the jet device and the cell capturing cavity in the first embodiment of the present application, the schematic diagram is shown in the figure;

[0035] Figure 3 When the jet device is increased with a flexible ring in the first embodiment of the present application, the assembly schematic diagram of the jet device and the cell capturing cavity is shown in the figure;

[0036] Figure 4 System module block diagram of the cell membrane jet perforation device of the first embodiment of the present application;

[0037] Figure 5 Structure diagram of the cell capturing cavities arranged on the same side of the cell introduction flow cavity in the second embodiment of the present application;

[0038] Figure 6 Structure diagram of the cell capturing cavities arranged in pairs on opposite sides of the cell introduction flow cavity in the second embodiment of the present application;

[0039] Figure 7 Flowchart of the cell membrane perforation method of the third embodiment of the present application. DETAILED DESCRIPTION

[0040] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this application.

[0041] Example 1

[0042] The first embodiment of the present invention provides a cell membrane jet perforation device, such as... Figure 1 As shown, it includes: a cell introduction flow chamber 1, a cell capture chamber 2, and a jet device 4.

[0043] Among them, such as Figure 1 As shown, firstly, the cell introduction flow chamber 1 has an inlet 11 and an outlet 12 located away from the inlet 11, allowing cells to fully develop within the flow chamber 1. Secondly, the cell capture chamber 2 is located between the inlet 11 and the outlet 12 and is connected to the cell introduction flow chamber 1. During the process of cells entering the cell introduction flow chamber 1 through the liquid flow from the inlet 11, the variable cross-section pipe vortex principle is utilized to allow the cells to follow the flow field of the liquid flow and enter the cell capture chamber 2, where they are captured.

[0044] In addition, such as Figure 1 As shown, in this embodiment, the jet device 4 provides a jet to the cell capture chamber 2, causing the cell membrane of the cells drawn into the cell capture chamber 2 to form at least one micropore.

[0045] Finally, by using negative pressure, cells in the cell capture chamber 2 can be obtained from the outlet 12 of the cell inlet flow chamber 1.

[0046] As can be seen from the above, by using the perforation device of this embodiment to mechanically perforate the cell membrane, one or more micropores can be formed on the cell membrane of the cells drawn out from the outlet 12 of the cell inlet flow chamber 1. At this time, macromolecules in the solution can enter the cell through the micropores under the action of external force. After the jet is completed, the cell membrane micropores heal themselves. At this time, negative pressure can be used to obtain the cells in the cell capture chamber from the outlet, thereby realizing the transmembrane transport of macromolecules.

[0047] Specifically, in this embodiment, such as Figure 1As shown in the figure, the bottom of the cell capture cavity 2 has a jet inlet 21. Moreover, the jet device 4 comprises a jet generating cavity 41, a vibrating component 45 and a driving component. The jet generating cavity 41 is connected with the jet inlet 21, and the side of the jet generating cavity 41 relative to the jet inlet 21 is not closed, forming an opening 42 coaxially arranged with the jet inlet 21. In addition, the vibrating component 45 is arranged on the side of the jet generating cavity 41 relative to the jet inlet 21, and the vibrating component 45 closes the opening 42. It should be noted that in the present embodiment, the vibrating component 45 can be fixed to the side of the jet generating cavity 41 relative to the jet inlet 21 in a detachable manner, for example, by using a locking member to lock the vibrating component 45 on the jet generating cavity 41. Finally, the driving component is connected with the vibrating component, and the driving component is used to drive the vibrating component to vibrate along the axis direction of the jet inlet 21.

[0048] In actual application, in combination with Figure 1 As shown in the figure, the vibrating component 45 can adopt a metal film, and the driving component can adopt a piezoelectric driving component. In the present embodiment, the piezoelectric driving component is a piezoelectric ceramic sheet 43 attached to the side of the vibrating component 45 away from the jet generating cavity 41. When the jet device 4 starts to work, a periodically changing voltage signal can be applied to the piezoelectric ceramic sheet 43, so that the piezoelectric ceramic sheet 43 can drive the vibrating component 45 to vibrate at high frequency along the axis direction of the jet inlet 21. At this time, the liquid in the jet generating cavity 41 is compressed and discharged into the cell capture cavity 2 through the jet inlet 21 of the cell capture cavity 2. It should be noted that the piezoelectric driving component in the present embodiment is only illustrated by taking the piezoelectric ceramic sheet 43 as an example, and in actual application, the piezoelectric driving component can also adopt other components with piezoelectric effect, and in the present embodiment, the type of the piezoelectric driving component is not limited.

[0049] In addition, as an alternative, the driving component can also adopt a driving component with mechanical properties, such as a syringe, which can push the vibrating component 45 to vibrate at high frequency, so that the liquid in the jet generating cavity 41 can be compressed to provide jet flow to the cell capture cavity 2. It should be noted that the vibrating component 45 mentioned in the present embodiment can adopt a vibrating film, for example, a metal film, a silicon film, a plastic film, etc. Alternatively, the vibrating component 45 can also adopt a vibrating block, and in the present embodiment, the type of the vibrating component 45 is not limited.

[0050] In addition, in order to enable the liquid in the jet generating cavity 41 to generate a strong impact flow when jetting into the cell capture cavity 2, in combination with Figure 2As shown, the jet flow generating chamber 41 has a part protruding along the axis of the jet flow inlet 21 relative to the side of the jet flow inlet 21, forming a jet flow pipe 44 connected with the jet flow inlet 21. And, by Figure 1 As can be seen, the jet flow pipe 44 is designed in a long and narrow structure, so that the inner diameter of the jet flow pipe 44 is extremely small. Therefore, when the liquid in the jet flow generating chamber 41 is jetted into the cell capturing chamber 2, the jet flow entering the cell capturing chamber 2 through the jet flow pipe 44 can form a strong impact to cause a strong vortex of the flow field, so that the cells in the cell capturing chamber 2 are subjected to a strong shear force of the flow field, thereby forming one or more micropores on the cell membrane to meet the subsequent transmembrane transport requirements of macromolecular particles of the cells. And, it should be noted that in the present embodiment, the jet flow pipe 44 and the side of the cell capturing chamber having the jet flow inlet 21 are fixed in a detachable manner, for example, as shown in Figure 1 As shown, the cell capturing chamber 2 has a part protruding along the axis of the jet flow inlet 21 relative to the side of the jet flow inlet 21, forming a pipe joint 23 connected with the jet flow pipe 44, and the pipe joint 23 is a threaded joint having an inner thread (not shown in the figure), and the jet flow pipe 44 has an inner surface 441 and an outer surface 442 opposite to the inner surface 441 around the axis thereof, and the outer surface 442 of the jet flow pipe 44 is provided with an outer thread (not shown in the figure) which can be screwed with the inner thread of the pipe joint 23, so that the jet flow pipe 44 and the pipe joint 23 are connected in a screwed manner. Of course, as an alternative, in some embodiments, the jet flow pipe 44 and the side of the cell capturing chamber 2 having the jet flow inlet 21 can also be fixed in a welded manner, and in the present embodiment, the connection manner between the jet flow pipe 44 and the cell capturing chamber 2 is not specifically limited. Or, in some embodiments, the cell capturing chamber 2, the jet flow generating chamber 41 and the jet flow pipe 44 can also be an integral whole.

[0051] And, in some embodiments, the end of the jet flow pipe 44 away from the jet flow inlet 21 is sharp-edged, i.e. the inlet side 444 of the inner surface 441 of the jet flow pipe 44 forms a sharp chamfer, so that the cutting of the liquid flow can be further accelerated, so that the cells in the cell capturing chamber 2 are subjected to a greater shear force of the vortex flow. As a preferred alternative, as shown in Figure 2As shown, the inner surface 441 of the jet pipe 44 gradually expands from one end to the other end of the jet inlet 21, so that the inner surface 441 of the entire jet pipe 44 is a conical surface structure, and the inside is approximately a conical channel. Therefore, when the liquid in the cell capture chamber 2 is injected into the cell capture chamber 2 through the jet pipe 44, the cell capture chamber 2 is affected by the liquid flow in a larger range, thereby further improving the shearing effect on the cells. Meanwhile, it is worth noting that when the jet pipe 44 and the jet inlet 21 are threadedly connected by the pipe joint 23, the length of the jet pipe 44 can be appropriately lengthened, as shown in Figure 3 so that the outlet side 443 of the jet pipe 44 can be in the same plane as the jet inlet 21, or the jet pipe 44 can also partially pass through the jet inlet 21, so that the outlet side 443 of the jet pipe 44 directly enters the inside of the cell capture chamber 2, and at the same time, as shown in Figure 3 the outlet side 443 of the jet pipe 44 is also provided with a flexible ring 6 along the axis direction of the jet pipe 44, and the flexible ring 6 gradually expands and bends from one side to the other side of the jet pipe 44. It is not difficult to see that, by virtue of the softness of the flexible ring 6, when the jet pipe 44 is connected to the pipe joint 23, the flexible ring 6 can be easily squeezed into the pipe joint 6, and when the flexible ring 6 enters the inside of the cell capture chamber 2 along with the outlet side 443 of the jet pipe 44, the flexible ring 6 can directly use its resilience to quickly expand to the initial state and abut against the peripheral part of the jet inlet 21 of the cell capture chamber 2, so that after the jet pipe 44 is connected to the pipe joint 23, the jet pipe 44 is not easy to rotate, which improves the connection firmness of the jet pipe 44 and the pipe joint 23, and by virtue of the expansion structure of the flexible pipe 6, the guiding performance of the liquid flow into the cell capture chamber 2 can be further improved, so that the cell capture chamber 2 is affected by the liquid flow in a wider range.

[0052] In addition, it is worth noting that, as shown in Figure 1 and Figure 4 In the present embodiment, the opening 42 of the jet generating chamber 41, the jet pipe 44 and the jet inlet 21 are coaxially arranged, but as a preferred arrangement, the opening 42 of the jet generating chamber 41, the jet pipe 44 and the jet inlet 21 can be coaxially arranged along the central axis direction of the cell capture chamber 2, so that the liquid flow emitted from the jet generating chamber 41 can directly enter the cell capture chamber 2 through the jet pipe 44, and at the same time, it is ensured that the cells entering the cell capture chamber 2 can continuously spiral around the central axis direction of the cell capture chamber 2 under the action of the vortex flow, effectively preventing the cells from adhering to the wall, so that the cells can better be subjected to the shearing force of the vortex flow, thereby improving the success rate of cell membrane perforation.

[0053] In addition, it should be noted that in order to precisely control the jet velocity, the cell membrane jet perforation device of the present embodiment further comprises a first syringe (not shown in the figure) connected to the inlet 11 of the cell introduction flow chamber 1 and a second syringe (not shown in the figure) connected to the outlet 12 of the cell introduction flow chamber, so that the cells can be pushed into the cell introduction flow chamber 1 from the inlet 11 by the pushing force of the first syringe and the cells in the cell capture chamber 2 can be sucked out from the outlet 12 by the second syringe when the cells are taken out. Of course, in actual application, the cells can also be taken out by separating the cell introduction flow chamber 1 and the cell capture chamber 2. At the same time, in order to precisely control the shear force of the fluid jetted by the jet device 4, as shown in Figure 4 the cell membrane jet perforation device of the present embodiment further comprises a main control module, and the main control module is in communication connection with the power supply device for applying voltage signals to the piezoelectric ceramic sheet 43 of the jet device 4, so that the power supply device can work under the control of the main control module, thereby achieving precise control of the fluid shear force.

[0054] Example Two

[0055] The second embodiment of the present application provides a cell membrane jet perforation device, which is further improved on the basis of the first embodiment, and the main improvement is that, as shown in Figure 5 and Figure 6 in the present embodiment, the cell introduction flow chamber and the driving jet device form a cell jet perforation module, and the cell introduction flow chamber 1 is provided with a plurality of cell jet perforation modules from the inlet 11 to the outlet 12, and the cell capture chambers 2 of the cell jet perforation modules are in communication with the cell introduction flow chamber 1.

[0056] As can be seen from the above, by providing a plurality of cell jet perforation modules, the transportation demand of high-throughput macromolecular substances can be met.

[0057] Specifically, in the present embodiment, as shown in Figure 5 each cell capture chamber 2 is sequentially arranged on the same side of the cell introduction flow chamber 1 from the inlet 11 to the outlet 12, that is, each cell capture chamber 2 is arranged on the same side of the cell introduction flow chamber. Of course, as shown in Figure 6 each cell capture chamber 2 is arranged opposite to each other from the inlet to the outlet 12 of the cell introduction flow chamber 1.

[0058] And it should be noted that in the present embodiment, each cell capture chamber 2 is only described by the above two arrangement methods, and in actual use, each cell capture chamber 2 can also be arranged in other ways on the cell introduction flow chamber 1 according to the transportation demand of the cells, which will not be described here.

[0059] Embodiment Three

[0060] The third embodiment of the present application provides a cell membrane perforation method, which uses the cell membrane jet flow perforation device as described in Embodiment One to perforate the cell membrane of a cell, and the method comprises the following steps as shown in the figure: Figure 7

[0061] Step 710, capture the cells from the inlet 11 into the cell introduction flow chamber 1 by the cell capture chamber 2.

[0062] Step 720, control the jet flow device 4 to provide a jet flow of a preset intensity into the cell capture chamber within a preset time length, so that the cell membrane of the cells sucked into the cell capture chamber 2 forms at least one micropore.

[0063] Step 730, use negative pressure to obtain the cells in the cell capture chamber 2 that have completed perforation and have completed the transmission of macromolecular substances from the outlet 12.

[0064] As can be seen from the above, by using the jet flow perforation method of the present embodiment to perforate the cell membrane, the cell is subjected to severe deformation when subjected to the shear force of the flow field, and one or more micropores are formed on the cell membrane. The extracellular macromolecular substances can directly pass through the micropores on the cell membrane, thereby realizing the high-throughput transmembrane transport of the macromolecular substances of the cell while minimizing the damage to the cell.

[0065] It should be noted that the present embodiment is an embodiment of the cell membrane jet flow perforation method corresponding to the first embodiment, and the present embodiment can be implemented in cooperation with the first embodiment. The technical details mentioned in the first embodiment are still valid in the present embodiment. In order to reduce repetition, they will not be described here. Correspondingly, the technical details mentioned in the present embodiment can also be applied to the first embodiment.

[0066] Embodiment Four

[0067] The fourth embodiment of the present application provides a macromolecular substance transmembrane transmission system, which comprises the cell membrane jet flow perforation device as described in the first or second embodiment.

[0068] Since the transmission system of the present embodiment comprises the cell membrane jet flow perforation device as described in the first or second embodiment, by perforating the cell membrane by the perforation device, the cell is subjected to severe deformation when subjected to the shear force of the flow field, and one or more micropores are formed on the cell membrane. The extracellular macromolecular substances can directly pass through the micropores on the cell membrane, thereby realizing the high-throughput transmembrane transport of the macromolecular substances of the cell while minimizing the damage to the cell.

[0069] ​Finally, it should be noted that, for a person having ordinary skill in the art, many technical details can be understood in order to make the reader better understand the present application. However, even without these technical details and based on various changes and modifications of the above-mentioned embodiments, the technical solutions claimed in the claims of the present application can be substantially realized. Therefore, in practical applications, various changes can be made to the above-mentioned embodiments in form and details without departing from the spirit and scope of the present application.

Claims

1. A cell membrane jet perforator device, characterized by, The application relates to a cell membrane jet flow perforation device. The cell membrane jet flow perforation device comprises: a cell introduction flow cavity; the cell introduction flow cavity has an inlet and an outlet arranged away from the inlet; a cell capturing cavity in communication with the cell introduction flow cavity, the cell capturing cavity being used for capturing cells entering the cell introduction flow cavity from the inlet; a jet flow device providing jet flow to the cell capturing cavity, so that the cell membrane of the cells in the cell capturing cavity forms at least one micropore; the bottom of the cell capturing cavity has a jet flow inlet; and the jet flow device comprises: a jet flow generating cavity connected with the jet flow inlet; one side of the jet flow generating cavity relative to the jet flow inlet is not closed, forming an opening coaxial with the jet flow inlet; a vibrating component arranged on the side of the jet flow generating cavity relative to the jet flow inlet, closing the opening; a driving component connected with the vibrating component, the driving component being used for driving the vibrating component to vibrate along the axis direction of the jet flow inlet; 2. The cell membrane jet perforator device of claim 1, wherein, a jet flow pipeline connected between the opening of the jet flow generating cavity and the jet flow inlet, the jet flow pipeline being in a long and narrow structure, so that when jet flow passes through the jet flow pipeline and enters the cell capturing cavity, strong vortex is formed and flow field shear force is generated; 3. The cell-membrane jet perforator device according to any one of claims 1-2, wherein, the opening of the jet flow generating cavity, the jet flow pipeline and the jet flow inlet are coaxial. The end of the jet flow pipeline away from the jet flow inlet is in an acute edge shape.

4. The cell membrane jet perforator device of claim 3, wherein, The vibrating component is a vibrating membrane. Alternatively, the vibrating component is a vibrating block.

5. The cell membrane jet perforator device of claim 1, wherein, The driving component is a piezoelectric driving component. Alternatively, the driving component is a driving component with mechanical properties.

6. The cell membrane jet perforator device of claim 5, wherein, The cell capturing cavity and the jet flow device form a cell jet flow perforation module; a plurality of cell jet flow perforation modules are arranged in the cell introduction flow cavity from the inlet to the outlet, and the cell capturing cavities of the cell jet flow perforation modules are in communication with the cell introduction flow cavity.

7. A method of cell membrane jet perforation, characterized by, The cell capturing cavities are arranged on the same side of the cell introduction flow cavity from the inlet to the outlet. Alternatively, the cell capturing cavities are arranged opposite to each other from the inlet to the outlet of the cell introduction flow cavity. The cell membrane jet flow perforation method comprises the following steps: cells entering the cell introduction flow cavity from the inlet are captured by the cell capturing cavity; 8. A transmembrane conduction system for macromolecules, characterized in that, the jet flow device is controlled to provide jet flow with a preset intensity to the cell capturing cavity within a preset time length, so that the cell membrane of the cells sucked into the cell capturing cavity forms at least one micropore; cells completing perforation and macromolecular substance transmission in the cell capturing cavity are obtained from the outlet by using negative pressure. The application further relates to a cell membrane jet flow perforation device. The cell membrane jet flow perforation device comprises: the cell membrane jet flow perforation device according to any one of claims 1-6.

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