Covid test card housing

Abstract

The application discloses a new crown test card shell, which comprises a circular bottom plate, isolation bands, detection fan-shaped areas, a dilution pipe and a detection sample backflow liquid collecting pipe; a plurality of isolation bands and detection fan-shaped areas are uniformly arranged on the upper end face of the circular bottom plate, the detection fan-shaped areas comprise sample pads, test paper strips and flow guide grooves; the test paper strip comprises at least seven kinds of coronavirus detection lines; the detection sample backflow liquid collecting pipe is internally provided with a water-absorbing cotton; a backflow port is arranged at the center of the circular bottom plate, and the detection sample backflow liquid collecting pipe is oppositely arranged at the bottom of the backflow port. Seven different coronavirus monoclonal antibody detection lines susceptible to human bodies are arranged on the test paper strip, so that a suspected coronavirus carrier can be detected for a certain kind of coronavirus at one time, and other kinds of coronaviruses are prevented from being misdetected as new coronavirus carriers.

Description

Technical Field

[0001] This invention belongs to the field of immunodiagnostic equipment, and more specifically, relates to a COVID-19 test card casing. Background Technology

[0002] The novel coronavirus belongs to the β genus. It is enveloped, and its particles are round or oval, often pleomorphic, with a diameter of 60-140 nm. COVID-19 testing can be divided into nucleic acid testing, antigen testing, and antibody testing, depending on the target. Nucleic acid and antigen tests target the virus, while antibody testing targets antibodies produced in the body. The two tests differ in their application scenarios and sampling sites—nucleic acid and antigen tests require sampling from the respiratory tract where the virus accumulates, such as nasopharyngeal swabs, sputum, or bronchoalveolar lavage fluid, while antibodies are produced in the blood and require blood sampling. Nucleic acid and antigen testing is used to determine whether a person carries the virus, while antibody testing is used to determine the person's infection status. Therefore, nucleic acid and antigen testing serve the same purpose, while antibody testing is complementary.

[0003] Chinese invention patent CN202010212667.3 discloses a kit for the combined detection of IgM and IgG antibodies against the 2019 novel coronavirus. The kit includes a dual-detection card and a detection buffer. The dual-detection card contains a 2019 novel coronavirus IgM antibody test strip and a 2019 novel coronavirus IgG antibody test strip. Each test strip includes a sample pad, a nitrocellulose membrane, and an absorbent pad. The sample pad is coated with anti-sheep erythrocyte antibodies, and the nitrocellulose membrane has a fluorescent antibody solid-phase line, a detection line, and a control line. Based on the antibody mechanism produced by the body against the novel coronavirus, this kit combines IgM and IgG antibody detection, avoiding false negatives and significantly improving the detection rate.

[0004] The shortcoming of existing patents lies in the fact that the mortality rate of COVID-19 infection is almost zero, and nucleic acid testing has become routine. COVID-19 test strips should not only detect the novel coronavirus of the β genus, but also detect other types of coronaviruses in the process of routine testing, thereby achieving precise identification of the specific coronavirus type infecting an individual. However, existing coronavirus test strips cannot distinguish the type of coronavirus infecting a single individual. Summary of the Invention

[0005] To address the problem that existing coronavirus test cards cannot distinguish the type of coronavirus infection in an individual, this invention provides a new coronavirus test card casing.

[0006] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0007] A COVID-19 test card casing includes a circular base plate, an isolation strip, a detection sector area, a dilution tube, and a test sample reflux collection tube;

[0008] The upper surface of the circular base plate is evenly provided with multiple isolation strips and detection sector areas, which include a sample pad, a test strip, and a flow guide groove.

[0009] The test strips include at least seven coronavirus detection lines, namely COVID-19, HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, and MERS-CoV coronavirus detection lines;

[0010] The sample reflux collection tube is lined with absorbent cotton.

[0011] A reflux port is provided at the center of the circular base plate, and a sample reflux liquid collection tube is placed directly opposite the bottom of the reflux port.

[0012] Furthermore, a connecting post that runs through the circular base plate is provided at the center of the circular base plate. The lower outer wall of the connecting post is provided with external threads, and the upper end of the sample reflux collection tube is provided with internal threads that mate with the external threads on the lower outer wall of the connecting post.

[0013] Furthermore, the inner wall of the upper end of the connecting column is provided with an internal thread, and the outer wall of the lower end of the dilution tube is provided with an external thread that mates with the internal thread on the inner wall of the upper end of the connecting column.

[0014] Furthermore, the dilution tube includes a tube body and a tube cap. The tube cap has a suction tube that penetrates through the middle of the tube cap. The upper end of the suction tube has a press-to-suction valve ball, and the lower end of the suction tube extends into the bottom of the tube body.

[0015] Furthermore, the sample pad is provided with a sample drop inlet, and the upper end of the sample drop inlet is provided with an inwardly sliding baffle along the outer side of the circular base plate.

[0016] Furthermore, the upper end of the circular base plate is inclined towards the center of the connecting column.

[0017] Furthermore, the circular base plate is made of PVC, and the circular base plate is bonded to the sample pad / test strip with hot melt adhesive.

[0018] Furthermore, a baffle plate is provided on the outer side of the circular base plate, and a support foot is provided at the bottom of the circular base plate.

[0019] Furthermore, a return port is provided at the end of the connecting column sidewall connecting the guide channel, and the guide channel has the same inclination as the upper surface of the circular base plate.

[0020] Furthermore, all seven coronavirus testing lines utilize color latex-labeled anti-coronavirus protein monoclonal antibodies.

[0021] The working principle of this invention is as follows: monoclonal antibodies (capture type) against seven coronaviruses are labeled with different colored latexes and sprayed onto the coronavirus detection lines of the test strip. During detection, if the test sample carries one of the seven coronaviruses, the monoclonal antibody can bind to the virus in the sample, causing it to carry the colored latex that displays that color. In chromatography, the monoclonal antibodies (detection type) coated on the detection lines of the seven coronaviruses bind to the virus and develop color. If the test sample does not contain any of the seven coronaviruses, none of the seven coronavirus detection lines will develop color.

[0022] The principle of this invention is as follows: Open the cap of the dilution tube, insert a cotton swab carrying the respiratory saliva of the person being tested into the tube and stir or let it stand for a period of time, then remove the cotton swab, insert it into the aspiration tube, and squeeze the aspiration valve ball at the top of the aspiration tube to draw up the diluted sample solution, drip it into the sample drop inlet on any sample pad in the detection sector area, close the sliding baffle, and wait for a period of time. Observe whether there is any change in the corresponding colored latex on the test strip in the detection sector area. Based on the change in different color bands, determine whether the person being tested carries one of the seven coronaviruses.

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] The test strips feature seven different monoclonal antibody detection lines for coronaviruses that are highly contagious to humans. A single test can identify a suspected coronavirus carrier as carrying only one specific coronavirus, preventing misdiagnosis of other coronaviruses as COVID-19.

[0025] At the same time, the device allows users to perform tests themselves, is easy to operate, and does not require nucleic acid testing by professional medical personnel in a hospital. Attached Figure Description

[0026] Figure 1 This is a top view of the outer shell structure of a COVID-19 test card according to the present invention;

[0027] Figure 2 for Figure 1 Sectional view at point AA;

[0028] Figure 3 This is a schematic diagram of the dilution tube structure of the present invention.

[0029] The markings in the diagram are as follows: 1-Circular base plate, 2-Isolation strip, 3-Detection sector area, 31-Sample pad, 32-Test strip, 33-Guide groove, 4-Dilution tube, 41-Tube body, 42-Tube cap, 5-Detection sample reflux collection tube, 6-7 Coronavirus detection lines, 7-Absorbent cotton, 8-Reflux port, 9-Connecting column, 10-Thread, 11-Suction tube, 12-Press suction valve ball, 13-Detection sample drop inlet, 14-Sliding baffle, 15-Liquid baffle plate, 16-Support foot. Detailed Implementation

[0030] To facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments and accompanying drawings. The content mentioned in the embodiments is not intended to limit the present invention.

[0031] like Figure 1 , 2 As shown in Figure 3, a COVID-19 test card casing includes a circular base plate 1, isolation strips 2, a detection fan-shaped area 3, a dilution tube 4, and a sample reflux collection tube 5. Multiple isolation strips 2 and detection fan-shaped areas 3 are evenly distributed on the upper surface of the circular base plate 1. The detection fan-shaped area 3 includes a sample pad 31, a test strip 32, and a guide groove 33. The isolation strips 2 are designed to prevent cross-contamination of different sample droplets within the detection fan-shaped area 3, which would make it impossible to determine which person being tested is infected with the coronavirus. The test strip 32 includes at least seven coronavirus detection lines 6, namely COVID-19, HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, and MERS-CoV coronavirus detection lines. The sample reflux collection tube 5 contains absorbent cotton 7. A reflux port 8 is located at the center of the circular base plate 1, with the sample reflux collection tube 5 positioned directly opposite the bottom of the reflux port 8.

[0032] This invention relates to a COVID-19 test card shell that can simultaneously detect whether multiple people are at risk of coronavirus infection. The shell's detection sector area has three sections, allowing for the testing of a large number of individuals. Specifically, the number of detection sector areas equals the number of people being tested simultaneously. When a COVID-19 test card shell shows a coronavirus reaction, a second round of testing is conducted on the individuals associated with that test card. Therefore, respiratory secretions from previously tested individuals need to be collected separately, or a second round of testing may be conducted on the individuals associated with that test card shell.

[0033] A connecting post 9, integrally extending through the circular base plate 1, is located at the center of the circular base plate 1. The lower outer wall of the connecting post 9 has an external thread 10. The upper end of the sample reflux collection tube 5 has an internal thread 10 that mates with the external thread 10 on the lower outer wall of the connecting post 9. The dilution tube 4 and the sample reflux collection tube 5 are connected via the connecting post 9. The sample reflux collection tube 5 collects and processes any unabsorbed sample solution from the detection sector 3.

[0034] The inner wall of the upper end of the connecting column 9 is provided with an internal thread 10, and the outer wall of the lower end of the dilution tube 4 is provided with an external thread 10 that mates with the internal thread 10 on the inner wall of the upper end of the connecting column 9. The connection via the thread 10 facilitates the recycling of used diluent and prevents virus-carrying liquid from leaking into the air and evaporating, which could lead to secondary infection of others.

[0035] The dilution tube 4 includes a tube body 41 and a cap 42. A suction tube 11, penetrating the middle of the cap 42, is provided. A press-to-suction valve ball 12 is located at the upper end of the suction tube 11, and the lower end of the suction tube 11 extends into the bottom of the tube body 41. The diluted sample solution to be tested can be fully aspirated or returned to the dilution tube 4 through the suction tube 11 connected to the cap 42 and the press-to-suction valve ball 12.

[0036] The sample pad 31 is provided with a sample drop inlet 13, and a sliding baffle 14 that pushes inward is provided at the upper end of the sample drop inlet 13 along the outer side of the circular base plate 1. The sample drop inlet 13 facilitates the directional dripping of the sample test solution; the sliding baffle 14 prevents the dripped sample test solution from leaking out of the sample drop inlet 13. When in use, after the sample test solution is dripped into the sample drop inlet 13, the sliding baffle 14 is immediately pushed to block the sample drop inlet 13. At the same time, the sliding baffle 14 is used to distinguish whether other sample test solutions have been dripped into the same test sector 3, preventing batch testing of samples from causing the tester to drip different test sample solutions into the same sample drop inlet 13, which could lead to misjudgment of the test results.

[0037] The upper end of the circular base plate 1 is inclined towards the center of the connecting column 9 to prevent the sample testing liquid from seeping back out of the entire COVID-19 test card shell.

[0038] The circular base plate 1 is made of PVC and is bonded to the sample pad 31 / test strip 32 using hot melt adhesive. The PVC material ensures that the entire COVID-19 test card casing can be recycled and processed at a designated location for reuse. The hot melt adhesive bonding of the circular base plate 1 to the sample pad 31 / test strip 32 allows for easy removal of the sample pad 31 and test strip 32 after a simple heating process, enabling the re-attachment of new sample pads 31 and test strips 32 for reuse. This reduces the generation of waste from COVID-19 test card casings and also facilitates reuse.

[0039] A liquid-repellent plate 15 is provided on the outer side of the circular base plate 1, and a support foot 16 is provided at the bottom of the circular base plate 1. The support foot 16 facilitates the user to place the COVID-19 test card shell horizontally when using the entire COVID-19 test card shell.

[0040] A return port 8 is provided at the end of the flow guide groove 33 on the side wall of the connecting column 9. The flow guide groove 33 has the same inclination as the upper surface of the circular base plate 1. The setting of the flow guide groove 33 ensures that the sample solution that is not completely absorbed by the sample pad 31 and the test strip 32 remains in the central area of ​​the upper end of the circular base plate 1. The excess sample solution flows into the test sample return liquid collection tube 5 at the bottom of the connecting column 9 through the return port 8, and is further absorbed by the water-absorbing cotton 7 set in the collection tube to prevent the sample test liquid from flowing back out of the return port 8 during the movement of the circular base plate 1.

[0041] All seven coronavirus detection lines 6 utilize color-labeled latex-labeled polyclonal antibodies against coronavirus proteins. The color-labeled latex of these antibodies detects different types of coronaviruses; different colors indicate that the person being tested carries that particular type of coronavirus.

[0042] The working principle of this invention is as follows: monoclonal antibodies (capture type) against seven coronaviruses are labeled with different colored latexes and sprayed onto the coronavirus detection lines of the test strip 32. During detection, if the test sample carries one of the seven coronaviruses, the monoclonal antibody can bind to the virus in the sample, causing it to carry the colored latex that displays that color. In chromatography, the monoclonal antibody (detection type) coated on the seven coronavirus detection lines 6 will bind and develop color. If the test sample does not contain any of the seven coronaviruses, the seven coronavirus detection lines 6 will not develop color.

[0043] The principle of this invention is as follows: Open the cap 42 of the dilution tube 4, insert a cotton swab carrying the respiratory saliva of the person being tested into the tube body 41 of the dilution tube 4 and stir or let it stand for a period of time, then take out the cotton swab, insert it into the aspiration tube 11, and squeeze the aspiration valve ball 12 at the upper end of the aspiration tube 11 to draw up the diluted sample solution, drip it into the sample drop inlet 13 set on any sample pad 31 in the detection sector area 3, close the sliding baffle 14, and wait for a period of time. Observe whether there is any change in the corresponding colored latex on the test strip 32 in the detection sector area 3. Based on the change in different color bands, determine whether the person being tested carries one of the seven coronaviruses.

[0044] The above provides a detailed description of a COVID-19 test card casing provided in this application. The specific embodiments described are merely for the purpose of helping to understand the method and core ideas of this application. It should be noted that those skilled in the art can make various improvements and modifications to this application without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of this application.

Claims

1. A COVID-19 test card casing, characterized in that, It includes a circular base plate (1), an isolation strip (2), a detection sector area (3), a dilution tube (4), and a sample reflux collection tube (5); The upper surface of the circular base plate (1) is evenly provided with multiple isolation strips (2) and detection fan-shaped areas (3). The detection fan-shaped areas (3) include a sample pad (31), a test strip (32) and a flow guide (33). The test strip (32) includes at least 7 coronavirus test lines (6), which are COVID-19, HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV and MERS-CoV coronavirus test lines; The sample reflux collection tube (5) is equipped with absorbent cotton (7); A connecting post (9) that runs through the circular base plate (1) is provided at the center of the circular base plate (1). The lower outer wall of the connecting post (9) is provided with an external thread. The upper end of the sample reflux collection tube (5) is provided with an internal thread that is connected to the external thread on the lower outer wall of the connecting post (9). The inner wall of the upper end of the connecting column (9) is provided with an internal thread, and the outer wall of the lower end of the dilution tube (4) is provided with an external thread that is connected to the internal thread on the inner wall of the upper end of the connecting column (9). A return port (8) is provided at the end of the guide channel (33) on the side wall of the connecting column (9). The guide channel (33) and the upper surface of the circular base plate (1) have the same inclination. The bottom of the return port (8) is directly opposite to the sample return liquid collection tube (5). The dilution tube (4) includes a tube body (41) and a tube cap (42). The tube cap (42) has a suction tube (11) that passes through the tube cap (42) in the middle. The upper end of the suction tube (11) is equipped with a press suction valve ball (12). The lower end of the suction tube (11) extends into the bottom of the tube body (41). The sample pad (31) is provided with a sample drop inlet (13), and the upper end of the sample drop inlet (13) is provided with an inwardly pushed sliding baffle (14) along the outer side of the circular base plate (1); The upper end of the circular base plate (1) is inclined towards the center of the connecting column (9); All seven coronavirus detection lines (6) used color latex-labeled anti-coronavirus protein monoclonal antibodies.

2. The COVID-19 test card casing according to claim 1, characterized in that, The circular base plate (1) is made of PVC material and is bonded to the sample pad (31) / test strip (32) by hot melt adhesive.

3. The COVID-19 test card casing according to claim 1, characterized in that, A baffle plate (15) is provided on the outside of the circular base plate (1), and a support foot (16) is provided at the bottom of the circular base plate (1).

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