A method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers

Abstract

The present invention discloses a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, which comprises the following steps: a. test plate design; b. test number design; c. sample collection; d. sample collection with plate number replacement; e. generation of original nucleic acid test reports. In the sample collection link of the nucleic acid detection test tube laboratory of the present invention, the test tube holes in 96 plates on the test plate correspond one by one to the test tube numbers, and in the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it is possible to clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate.

Description

Technical Field

[0001] The present invention relates to a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers. Background Art

[0002] Under the current impact of the epidemic, when facing nucleic acid sampling, especially large-scale nucleic acid sampling, barcode labels with a certain arrangement rule are usually pasted on test tubes for easy identification and classification statistics.

[0003] Currently, the received test tubes are usually placed on a test plate. The test tubes on the same test plate are usually taken out for testing at the same time when put into the laboratory for detection. When a positive case is found, it is impossible to accurately know the information of the specific relevant nucleic acid sampling personnel from the current positive test tube, but it is necessary to screen from the numerous sampling personnel on the entire test plate, greatly increasing the screening scope. At the same time, for personnel nucleic acid sampling and environmental sampling, they are currently separately counted in the input system, and it is also inconvenient to record. Summary of the Invention

[0004] The main technical problem to be solved by the present invention is to provide a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers. In the process of receiving samples of nucleic acid detection test tubes in the laboratory, the test tube holes in the 96 plates on the test plate correspond one by one with the test tube numbers. In the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it is possible to clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate.

[0005] To solve the above technical problem, a technical solution adopted by the present invention is: to provide a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, including the following steps:

[0006] a Test plate design: Design the test plate as an 8*12 matrix. Its position number and the test tube holes in the plate are arranged in 8 rows from top to bottom and use the A-H sequence, and are arranged in 12 columns from left to right and use the 1-12 sequence;

[0007] b Test number design: Use the laboratory number to encode each test tube. The laboratory number is composed of the "plate number" and the "position number". The last two digits of the test number are the "position number", and the first n digits after removing the last two digits are the "plate number";

[0008] c Sample receiving: When receiving samples, input the initial laboratory number to start receiving samples. By scanning the one-dimensional barcode on the test tube, enter the test tube number. The laboratory number is sequentially incremented during the sample receiving process, so that the laboratory number corresponds one by one with the test tube number, and then the test tube number also corresponds one by one with the position of the test tube hole in the plate on the test plate;

[0009] Sample collection for d plate number replacement: End the sample collection for the current plate number, automatically enter the next plate, re-enter the inspection number to start sample collection, and repeat the above steps;

[0010] e Generation of nucleic acid test original single report: The system automatically generates the nucleic acid test original single for the completed plate number of sample collection, fills the test tube numbers in the corresponding test tube holes of the test plate, automatically calculates the sample numbers, and forms a report for export.

[0011] In a preferred embodiment of the present invention, in step b, the plate numbers of the laboratory numbers are not repeated from 00:00 to 24:00 every day, and the plate numbers start to be recounted at 00:00 the next day. Check whether the plate number exists within the day. If it exists, prompt "This plate number already exists today. Please correct the inspection number" after entering the inspection number.

[0012] In a preferred embodiment of the present invention, in step c, the number of samples collected for each test plate is not more than 96. When the number of samples collected exceeds the limit, trigger the inspection rule to prompt "The number of samples collected for this plate has reached 96, and sample collection cannot continue".

[0013] In a preferred embodiment of the present invention, in step c, the sample collection of the test plate includes the collection of samples for the whole population's nucleic acid and environmental samples. Among them, the numbers of environmental samples are marked with line breaks in the cells or marked with the word "environment" at the end.

[0014] The beneficial effects of the present invention are as follows: A method for automatically and uniquely corresponding nucleic acid detection sample numbers and experimental plate numbers pointed out by the present invention. In the sample collection link of the nucleic acid detection test tube laboratory, the test tube holes in the 96 plates on the test plate correspond one by one with the test tube numbers. In the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it can clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to these drawings, where:

[0016] Figure 1 It is a schematic diagram of the inspection number of a preferred embodiment of a method for automatically and uniquely corresponding nucleic acid detection sample numbers and experimental plate numbers of the present invention;

[0017] Figure 2 It is a schematic diagram of the nucleic acid detection original single of a preferred embodiment of a method for automatically and uniquely corresponding nucleic acid detection sample numbers and experimental plate numbers of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0019] Please refer to Figure 1 as shown, the embodiments of the present invention include:

[0020] A method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, comprising the following steps:

[0021] a. Test plate design: Design the test plate as an 8*12 matrix. Its position number and the test tube holes in the plate are arranged in 8 rows from top to bottom and use the A-H sequence, and are arranged in 12 columns from left to right and use the 1-12 sequence;

[0022] b. Test number design: Use the laboratory number to encode each test tube. The laboratory number is composed of the "plate number" and the "position number". The last two digits of the test number are the "position number", and the first n digits after removing the last two digits are the "plate number"; the plate numbers of the laboratory numbers do not repeat from 00:00 to 24:00 every day, and the plate number starts to be recounted at 00:00 the next day. For example, the plate numbers on February 20 are 1-46, and starting from 00:00 on February 21, the plate number can start to be recounted from 1;

[0023] Verify whether the plate number exists within the day. If it exists, prompt "This plate number already exists today. Please correct the test number" after entering the test number;

[0024] c. Sample collection: When collecting samples, start collecting samples by entering the initial laboratory number. By scanning the one-dimensional barcode on the test tube, enter the test tube number. The laboratory number is accumulated sequentially during the sample collection process, so that the laboratory number corresponds to the test tube number one by one, and then the test tube number also corresponds to the position of the test tube hole in the test plate one by one;

[0025] The sample collection quantity of each test plate is not greater than 96. When the sample collection quantity is exceeded, trigger the test rule to prompt "The sample collection quantity of this plate has reached 96 and sample collection cannot continue";

[0026] The sample collection of the test plate includes national nucleic acid sample collection and environmental sample collection. Among them, the number of environmental sample collection is marked with a line break in the cell or marked with the word "ring" at the end;

[0027] d. Plate number replacement for sample collection: End the sample collection of the current plate number, automatically enter the next plate, re-enter the test number to start sample collection, and repeat the above steps;

[0028] In addition, for example, the actual test tube placement positions on Plate 1 are from 02 to 35 and from 37 to 96. Then, set the starting inspection number as 102. When receiving samples up to 135, it is necessary to reset the starting inspection number to 137 and continue receiving samples up to 196. Leave the positions without received samples blank and click "Finish" to complete the sample receiving for this plate. The sample receiving format is XXXX-XXXX, which is the starting sample number (position number on the plate) - the ending sample number (position number on the plate) set for this plate, without considering the situation of blank sample numbers in the middle. For example, when starting to receive samples with inspection number 1002 and ending with 1096 for this plate, and 1036 is not received in the middle, the sample number field still shows "1002-1096".

[0029] During the sample receiving process, click the close button on the sample scanning and receiving window. It does not end this plate and continue to the next plate. Re-enter the sample scanning and receiving window to continue receiving samples for this plate (the inspection number needs to be reset).

[0030] During the sample receiving process of Plate 1, if the inspection number is reset but the corresponding plate number is not Plate 1, a prompt "The currently set plate number has changed. Please first end the sample receiving for Plate XX" will be displayed. For example, starting to receive samples from 102 up to 130, and then resetting the inspection number to 232, a prompt "The currently set plate number has changed. Please first end the sample receiving for Plate 1" will be displayed when entering the inspection number 232.

[0031] Generation of the original nucleic acid test form: The system automatically generates the original nucleic acid test form for the plate with completed sample receiving, fills the test tube numbers in the corresponding test tube holes on the test plate, automatically calculates the sample numbers, and forms a report for export.

[0032] In summary, a method for automatically and uniquely corresponding nucleic acid detection sample numbers and experimental plate numbers pointed out by the present invention. In the sample receiving link of the nucleic acid detection test tube laboratory, the 96 test tube holes on the test plate correspond one by one with the test tube numbers. In the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it can clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate.

[0033] The above are only embodiments of the present invention and do not limit the patent scope of the present invention. Any equivalent structural or equivalent process transformation made using the content of the specification of the present invention, or directly or indirectly applied in other related technical fields, shall be included in the patent protection scope of the present invention by the same token.

Claims

1. A method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, characterized in that, it includes the following steps: a. Test plate design: Design the test plate as an 8*12 matrix. The position numbers and the test tube holes in the plate are arranged in 8 rows from top to bottom and use the A-H sequence, and are arranged in 12 columns from left to right and use the 1-12 sequence; b. Test number design: Use the laboratory number to encode each test tube. The laboratory number is composed of the "plate number" and the "position number". The last two digits of the laboratory number are the "position number", and the first n digits after removing the last two digits are the "plate number"; c. Sample collection: When collecting samples, start collecting samples by inputting the initial laboratory number. By scanning the one-dimensional barcode on the test tube, the test tube number is entered, and the laboratory number is incremented sequentially during the sample collection process, so that the laboratory number corresponds to the test tube number one by one, and then the test tube number also corresponds to the position of the test tube hole in the test plate one by one; d. Sample collection with plate number replacement: End the sample collection of the current plate number, automatically enter the next plate, re-enter the laboratory number to start sample collection, and repeat the above steps; e. Generation of the original nucleic acid test form: The system automatically generates the original nucleic acid test form for the completed plate number of sample collection, fills the test tube number in the corresponding test tube hole in the test plate, automatically calculates the sample number, and forms a report for export.

2. The method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers according to claim 1, characterized in that, in step b, the plate numbers of the laboratory numbers do not repeat from 00:00 to 24:00 every day, and the plate number is re-counted starting from 00:00 the next day. Check whether the plate number exists within the day. If it exists, prompt "This plate number already exists today. Please correct the laboratory number" after inputting the laboratory number.

3. The method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers according to claim 1, characterized in that, in step c, the number of samples collected for each test plate is not greater than 96. When the number of samples collected exceeds the limit, trigger the test rule to prompt "The number of samples collected for this plate has reached 96, and sample collection cannot continue".

4. The method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers according to claim 1, characterized in that, in step c, the sample collection of the test plate includes universal nucleic acid sample collection and environmental sample collection. Among them, the numbers of environmental sample collection are marked with line breaks in the cell or marked with the word "Ring" at the end.

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