A cell culture dish for crayfish hemolymph experiments

Abstract

The present invention provides a cell culture dish for crayfish hemolymph experiments. The limiting component includes a snap ring, a rubber body, a pipe, a rotating sphere, an inclined rod, and a clamping block. The snap ring is arranged inside the frame, the rubber body is embedded in the inner wall of the snap ring, the pipe penetrates through the inside of the snap ring, the rotating sphere is hinged to the inner wall of the pipe away from the rubber body, the inclined rod swings at one end of the rotating sphere, the clamping block slides at one end of the inclined rod, and the air inside the rubber body quickly enters the inside of the pipe, facilitating the rapid compression of the air to impact the side surface of the inclined rod. At this time, the inclined rod can swing at an angle through the rotating sphere, and the inclined rod assists the clamping block to slide horizontally to the outside of the pipe. The clamping block can be clamped inside the groove on the outside of the dish body. At this time, the clamping block can be used to limit the dish body, avoiding the situation of the dish body shaking when the whole frame moves.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic animal cell detection, and particularly relates to a cell culture dish for crayfish hemolymph experiments. Background Art

[0002] In aquatic crustaceans, microbial polysaccharides and other pathogen-associated molecular patterns can prompt the release of pro-phenoloxidase from blood cells, and then activate phenoloxidase-activating enzyme through a serine protease cascade reaction to activate phenoloxidase, generating melanin to cause the body's immune response. This is a typical phenoloxidase activation system, which is an extremely important component of the innate immune system of invertebrates including crustaceans. In crayfish hemolymph experiments, crayfish hemolymph is processed into cell sections, and the cell sections are cultured using a cell culture dish to observe the immune response caused by the interaction. Regarding the technical inspiration for the cell culture dish;

[0003] The following problems have been found in the research on cell culture dishes:

[0004] Since the cell section has a small shape, while the cell culture dish has a large volume, and the cell culture dish can only culture a single cell section at a time. At the same time, the cell culture dish can only place the cell section, and subsequent instruments are needed to assist in cell culture, resulting in the cell culture dish being unable to perform warm water-assisted culture on multiple cell sections simultaneously. At the same time, when the cell culture dish is taken and placed, the whole cell culture dish is prone to shaking, which is likely to affect the culture of the cell section inside the cell culture dish;

[0005] Currently, the prior art CN201811319254.4, a cell culture device for cell heating experiments, discloses a culture device. This invention is used to fixedly place culture dishes of different diameters. By arranging a plurality of heating plates under a plurality of culture dishes, it can heat the cells in each culture dish and can be displayed by a mechanical thermometer. According to the temperature required by the experimenter for cell experiments, the operation is convenient. It can achieve different heating times for each heating unit, and multiple experiments or sample repetitions can be carried out simultaneously. In addition, the mechanical thermometer is used to display the temperature, which is heat-resistant and can reduce the damage of temperature to the thermometer;

[0006] The present invention can mainly solve the problem that the cell culture dish cannot perform warm water-assisted culture on multiple cell sections simultaneously. Summary of the Invention

[0007] To solve the above technical problems, the present invention provides a cell culture dish for crayfish hemolymph experiments to solve the problems described in the above background art.

[0008] The purpose and efficacy of a cell culture dish for crayfish hemolymph experiments of the present invention are achieved by the following specific technical means: A cell culture dish for crayfish hemolymph experiments includes a frame, and a sealing cover is movably nested and connected to the upper end of the frame. A tray is movably nested at the lower end of the frame, and a culture component is movably nested at one end of the frame close to the sealing cover. The culture component includes a dish body and a convex block. The dish body is the main body of the culture dish, and the convex block is arranged in a semi-spherical shape.

[0009] Further, a groove is opened at the upper end of the frame close to the sealing cover. There are multiple grooves, and the convex block is movably nested with the groove. The groove is vertically penetrated with the inside of the tray. The dish body is movably nested with the frame through the convex block, and a limiting component for further limiting the dish body is arranged inside the groove of the frame.

[0010] Further, the inside of the tray is arranged in a concave shape, and the lower end of the frame is movably nested with the concave edge of the tray.

[0011] Further, the dish body and the convex block are provided in a matching manner. The dish body is arranged in a concave shape, and a cell patch is placed inside the dish body. Grooves are opened on the outer side of the upper end of the dish body, and 3 - 4 groups of grooves are arranged vertically.

[0012] Further, holes are opened at the lower end and both sides of the convex block, and the inside of the convex block is in a hollow shape. The number of grooves of the convex block, the dish body and the frame are provided in a matching manner.

[0013] Further, the limiting component includes a snap ring, a rubber body, a pipe, a rotating sphere, an inclined rod and a block. The snap ring is arranged inside the frame, the rubber body is embedded in the inner wall of the snap ring, the pipe penetrates through the inside of the snap ring, the rotating sphere is hinged to the inner wall of one end of the pipe away from the rubber body, the inclined rod swings at one end of the rotating sphere, and the block slides at one end of the inclined rod.

[0014] Further, the snap ring is vertically sleeved with the upper groove of the frame, the convex block is embedded inside the snap ring through the upper groove of the frame, the inside of the snap ring is in a hollow shape, and the snap ring is arranged in a circular ring shape.

[0015] Further, the inside of the rubber body is filled with air, and the rubber body is arranged in a spherical shape as a whole. The thickness of the inner wall of the rubber body is less than 0.4 cm. The side of the rubber body away from the inner wall of the snap ring is vertically pressed against the convex block. The rubber body is arranged in a cross shape when viewed from above on the inner wall of the snap ring, and the rubber body is made of rubber material.

[0016] Further, the pipe is arranged in a semi - crescent shape, that is, half of the crescent shape. The specific shape can be referred to the attached Figure 4 to the specification. One end of the pipe away from the rubber body extends to the upper end of the inner wall of the snap ring, and the pipe and the rubber body are provided in a matching manner.

[0017] Further, the rotating sphere and the inclined rod are provided in a matching manner. The inclined rod is inclined at 15 - 45°, and one end of each inclined rod is connected to the clamping block. The outer side of the clamping block is slidably and fittingly nested with the inner wall of the pipe. One end of the clamping block is slidably nested with the outer groove of the dish body. The width of the inclined rod is greater than 1 cm.

[0018] Further, a flow guide frame penetrates through the middle of the inside of the tray. Both ends of the flow guide frame penetrate through elbow pipes. One end of each elbow pipe penetrates through a through pipe. A disc is wound around the outside of the through pipe. A baffle is inlaid on the inner wall of the upper end of the through pipe.

[0019] Further, the flow guide frame is arranged in a concave shape, extends to both sides at the lower end of the frame, warm water is filled inside the flow guide frame, and the flow guide frame is communicated with the through pipe through the elbow pipe.

[0020] Further, the elbow pipe is arranged in an "S" shape, and the number of the elbow pipes and the through pipes is provided in a matching manner. The through pipe extends to the upper end of the disc, and the upper end of the through pipe is vertically butted with the hole at the lower end of the convex block.

[0021] Further, the disc is arranged in an annular shape. One end of the elbow pipe penetrates into the inside of the disc, and the disc is provided in a matching manner with the through pipe.

[0022] Further, a plurality of baffles are wound around the inner wall of the through pipe. The whole baffle is arranged in an inverted "V" shape. The thickness of the baffle is 0.1 - 0.15 cm, and the material of the baffle is the same as that of the rubber body.

[0023] Beneficial effects:

[0024] 1. Manually embed the convex block into the groove inside the frame. Since the snap ring is vertically sleeved with the upper groove of the frame, the convex block can be embedded into the inner side of the snap ring through the upper groove of the frame. At this time, the outer groove of the dish body is horizontally corresponding to the clamping block, and the outer side of the lower end of the convex block can be pressed against the upper end of the side of the rubber body, and the side of the rubber body is deformed as a whole under the extrusion.

[0025] 2. By using the pipe arranged in a semi - lunar shape, that is, half of the lunar shape, when the side of the rubber body is extruded, the air inside the rubber body can quickly enter the inside of the pipe, which is convenient for the air to be quickly compressed and impact the side of the inclined rod. At this time, the inclined rod can swing at an angle through the rotating sphere, and the inclined rod assists the clamping block to slide horizontally to the outside of the pipe. The clamping block can be clamped inside the outer groove of the dish body. At this time, the clamping block can be used to limit the position of the dish body, avoiding the situation that the dish body shakes when the whole frame moves.

[0026] 3. At the same time, the bump is in a linkage state with the air inside the rubber body and the clamping block. Therefore, when the dish body is manually lifted upwards, the clamping block at the lower end of the dish body cannot squeeze the rubber body, and the air inside the rubber body cannot impact one end of the inclined rod. At this time, the rubber body can slowly deform and return to its original position, and the inclined rod at one end inside the pipeline can drive the clamping block to retract into the pipeline through the rotating sphere, achieving the effect of conveniently and quickly taking out the dish body;

[0027] 4. When the bump is embedded inside the frame, the through pipe is vertically sleeved with the hole at the lower end of the bump. Warm water enters the inside of the elbow through the diversion frame, and the warm water enters the inside of the through pipe through the elbow in sequence. Since the frame is embedded at the upper end of the tray, at this time, warm water is manually filled at both ends of the diversion frame, and the lower end of the frame limits the upper space of the diversion frame, resulting in a limited inner diameter of the diversion frame. As the warm water is continuously injected, the warm water can flow upwards inside the through pipe. When the warm water flows to the lower end inside the through pipe, using the thickness of the baffle plate of 0.1 - 0.15 cm, it can conveniently drive the baffle plate to bend and deform at an angle, thereby facilitating the warm water to enter the upper end inside the through pipe. The warm water can enter the inside of the bump through the through pipe, and the warm water inside the bump can assist in culturing the cell sections inside the dish body. Brief Description of the Drawings

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0029] Figure 2 It is a schematic diagram of the frame structure of the present invention.

[0030] Figure 3 It is a schematic cross-sectional view of the frame of the present invention.

[0031] Figure 4 It is a schematic cross-sectional view of the snap ring of the present invention.

[0032] Figure 5 For the present invention Figure 4 It is an enlarged schematic diagram of part A in the present invention.

[0033] Figure 6 It is a schematic diagram of the tray structure of the present invention.

[0034] Figure 7 It is a cross-sectional view of the connection between the through pipe and the bump of the present invention.

[0035] Figures 1-7 In the present invention, the corresponding relationship between the component names and the drawing reference numerals is as follows:

[0036] 1 - Frame, 101 - Sealing cover, 102 - Tray, 103 - Dish body, 104 - Bump, 2 - Snap ring, 201 - Rubber body, 202 - Pipe, 3 - Rotating sphere, 301 - Diagonal bar, 302 - Block, 4 - Flow - guiding frame, 401 - Elbow pipe, 402 - Disc, 403 - Through - pipe, 404 - Baffle. Detailed implementation manners

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0038] Embodiment:

[0039] As shown in the attached Figure 1 to the attached Figure 7 figure:

[0040] Embodiment 1: A cell culture dish for crayfish hemolymph experiments, including a frame 1. The upper end of the frame 1 is movably nested and connected with a sealing cover 101. The lower end of the frame 1 is movably nested with a tray 102. One end of the frame 1 close to the sealing cover 101 is movably nested with a culture component. The culture component includes a dish body 103 and a bump 104. The dish body 103 is the main body of the culture dish, and the bump 104 is arranged in a semi - spherical shape.

[0041] Among them: For the frame 1 and the sealing cover 101, a groove is opened at the upper end of the frame 1 close to the sealing cover 101. There are multiple grooves. The bump 104 is movably nested with the groove. The groove is vertically penetrated with the inside of the tray 102. The dish body 103 is movably nested with the frame 1 through the bump 104. And a limiting component for further limiting the dish body 103 is arranged inside the groove of the frame 1.

[0042] Tray 102, the inside of the tray 102 is concave - shaped, and the lower end of the frame 1 is movably nested with the concave - shaped edge of the tray 102.

[0043] Dish body 103, the dish body 103 and the bump 104 are set in a matching manner. The dish body 103 is concave - shaped. A cell patch is placed inside the dish body 103. Grooves are opened on the outer side of the upper end of the dish body 103, and 3 - 4 groups of grooves are arranged vertically.

[0044] Bump 104, holes are opened at the lower end and both sides of the bump 104. The inside of the bump 104 is hollow - shaped. The number of grooves of the bump 104 and the dish body 103 is set to match the number of grooves of the frame 1.

[0045] The cell patches are respectively placed inside the dish body 103. The bump 104 is snap-fitted to the inner side of the frame 1. The dish body 103 is snap-fitted to the upper end of the frame 1 through the bump 104. The sealing cover 101 is movably nested on the upper end of the frame 1, and the sealing cover 101 forms a closed seal for the dish body 103.

[0046] Example 2: Refer to the appendix of the specification Figures 1-5 It can be known that the difference between Example 2 and Example 1 is that the limiting component includes a snap ring 2, a rubber body 201, a pipe 202, a rotating sphere 3, an inclined rod 301, and a clamping block 302. The snap ring 2 is arranged inside the frame 1. The rubber body 201 is embedded in the inner wall of the snap ring 2. The pipe 202 penetrates through the inside of the snap ring 2. The rotating sphere 3 is hinged to the inner wall of one end of the pipe 202 away from the rubber body 201. The inclined rod 301 swings at one end of the rotating sphere 3. The clamping block 302 slides at one end of the inclined rod 301.

[0047] Among them: the snap ring 2, the snap ring 2 is vertically sleeved with the upper groove of the frame 1. The bump 104 is embedded in the inner side of the snap ring 2 through the upper groove of the frame 1. The inside of the snap ring 2 is hollow. The snap ring 2 is circularly arranged.

[0048] The snap ring 2 facilitates limiting the lower end of the dish body 103. The snap ring 2 is provided in a matching manner with the upper groove of the frame 1.

[0049] The rubber body 201, the inside of the rubber body 201 is filled with air, and the rubber body 201 is integrally spherical. The inner wall thickness of the rubber body 201 is less than 0.4 cm. The side of the rubber body 201 away from the inner wall of the snap ring 2 is vertically pressed against the bump 104. The rubber body 201 is arranged in a cross-shaped layout when viewed from above on the inner wall of the snap ring 2. The rubber body 201 is made of rubber material.

[0050] The inner wall thickness of the rubber body 201 is less than 0.4 cm to avoid the inconvenience of deformation of the rubber body 201 after being pressed by the bump 104 due to the thickness of the rubber body 201 being greater than 0.4 cm.

[0051] The pipe 202, the pipe 202 is arranged in a half-moon shape, that is, half of the moon shape. For the specific shape, refer to the appendix of the specification Figure 4 , one end of the pipe 202 away from the rubber body 201 extends to the upper end of the inner wall of the snap ring 2. The pipe 202 and the rubber body 201 are provided in a matching manner.

[0052] The pipe 202 is arranged in a half-moon shape, that is, half of the moon shape. For the specific shape, refer to the appendix of the specification Figure 4 , by using the shape setting of the pipe 202, when the side of the rubber body 201 is pressed, the air inside the rubber body 201 quickly enters the inside of the pipe 202, which is convenient for the air to be quickly compressed and impact the side of the inclined rod 301. The air compression principle can be referred to.

[0053] The diagonal rod 301 and the rotating sphere 3 are provided in a matching manner. The diagonal rod 301 is inclined at an angle of 15 - 45°. One end of each diagonal rod 301 is connected to a clamping block 302. The outer side of the clamping block 302 is slidably and fittingly nested with the inner wall of the pipe 202. One end of the clamping block 302 is slidably nested with the outer groove of the dish body 103. The width of the diagonal rod 301 is greater than 1 cm;

[0054] The width of the diagonal rod 301 being greater than 1 cm can increase the contact area between the diagonal rod 301 and the air inside the pipe 202;

[0055] Among them: Manually embed the convex block 104 into the groove inside the frame 1. Since the clamping ring 2 is vertically sleeved with the upper groove of the frame 1, the convex block 104 can be embedded into the inner side of the clamping ring 2 through the upper groove of the frame 1. At this time, the outer groove of the dish body 103 is horizontally corresponding to the clamping block 302. The outer side of the lower end of the convex block 104 can be pressed against the upper end of the side of the rubber body 201, and the side of the rubber body 201 is deformed as a whole under the extrusion;

[0056] Taking advantage of the pipe 202 being arranged in a semi - lunar shape, that is, half of the lunar shape, when the side of the rubber body 201 is extruded, the air inside the rubber body 201 can quickly enter the inside of the pipe 202, facilitating the rapid compression of the air to impact the side of the diagonal rod 301. At this time, the diagonal rod 301 can swing at an angle through the rotating sphere 3. The diagonal rod 301 assists the clamping block 302 to slide horizontally to the outside of the pipe 202, and the clamping block 302 can be clamped inside the outer groove of the dish body 103. At this time, the clamping block 302 can be used to limit the dish body 103, avoiding the situation where the dish body 103 shakes when the whole frame 1 moves;

[0057] At the same time, taking advantage of the convex block 104 being in a linkage state with the clamping block 302 through the air inside the rubber body 201, when the dish body 103 is manually taken up, the lower clamping block 302 of the dish body 103 cannot form extrusion on the rubber body 201, and the air inside the rubber body 201 cannot impact one end of the diagonal rod 301. At this time, the rubber body 201 can slowly deform and return to its original position, and the diagonal rod 301 at one end inside the pipe 202 can drive the clamping block 302 to retract into the inside of the pipe 202 through the rotating sphere 3, achieving the effect of facilitating the rapid removal of the dish body 103;

[0058] Example 3: Refer to the attached Figure 6 and 7 It can be known that the difference between Example 3 and Examples 1 and 2 is that a flow - guiding frame 4 penetrates through the middle of the tray 102. Both ends of the flow - guiding frame 4 are penetrated by elbow pipes 401. One end of the elbow pipe 401 is penetrated by a through - pipe 403. A disc 402 is wound around the outside of the through - pipe 403. A baffle 404 is inlaid on the inner wall of the upper end of the through - pipe 403;

[0059] Among them: the flow guide frame 4, the flow guide frame 4 is arranged in a concave shape, the flow guide frame 4 extends to both sides of the lower end of the frame 1, warm water is filled inside the flow guide frame 4, and the flow guide frame 4 communicates with the through pipe 403 through the elbow pipe 401;

[0060] The elbow pipe 401 and the through pipe 403, the elbow pipe 401 is arranged in an "S" shape, the elbow pipe 401 and the through pipe 403 are arranged in a number matching the number of bumps 104, the through pipe 403 extends to the upper end of the disc 402, and the upper end of the through pipe 403 is vertically docked with the hole at the lower end of the bump 104;

[0061] When a small amount of warm water flows downward inside the through pipe 403, by using the "S" shape of the elbow pipe 401, the elbow pipe 401 can prevent the warm water from flowing downward back into the inside of the flow guide frame 4, achieving the effect of short-term water storage;

[0062] The disc 402, the disc 402 is arranged in an annular shape, one end of the elbow pipe 401 penetrates into the inside of the disc 402, and the disc 402 is arranged in a matching manner with the through pipe 403;

[0063] The baffle 404, multiple baffles 404 surround the inner wall of the through pipe 403, the baffle 404 is arranged in an inverted "V" shape as a whole, the thickness of the baffle 404 is 0.1 - 0.15 cm, and the material of the baffle 404 is the same as the material of the rubber body 201;

[0064] The baffle 404 is arranged in an inverted "V" shape as a whole, and the baffle 404 can prevent the warm water above the inside of the through pipe 403 from flowing back to the lower end inside the through pipe 403;

[0065] When the warm water flows to the lower end inside the through pipe 403, by using the thickness of the baffle 404 being 0.1 - 0.15 cm, it can facilitate the warm water to drive the baffle 404 to bend and deform at an angle, and then facilitate the warm water to enter the upper end inside the through pipe 403;

[0066] Among them: when the bump 104 is embedded inside the frame 1, the through pipe 403 is vertically sleeved with the hole at the lower end of the bump 104, the warm water enters the inside of the elbow pipe 401 through the flow guide frame 4, and the warm water enters the inside of the through pipe 403 through the elbow pipe 401 in sequence. Since the frame 1 is embedded at the upper end of the tray 102, at this time, manually fill warm water at both ends of the flow guide frame 4, and the lower end of the frame 1 forms a limit for the upper end space of the flow guide frame 4, resulting in a limited inner diameter inside the flow guide frame 4. As the warm water is continuously injected, the warm water can flow upward inside the through pipe 403. When the warm water flows to the lower end inside the through pipe 403, by using the thickness of the baffle 404 being 0.1 - 0.15 cm, it can facilitate the warm water to drive the baffle 404 to bend and deform at an angle, and then facilitate the warm water to enter the upper end inside the through pipe 403. The warm water can enter the inside of the bump 104 through the through pipe 403, and the warm water inside the bump 104 can be used to assist in culturing the cell sections inside the dish 103.

Claims

1. A cell culture dish for crayfish hemolymph experiments, comprising a frame (1), characterized in that: A sealing cover (101) is movably nested and connected to the upper end of the frame (1), a tray (102) is movably nested at the lower end of the frame (1), and a culture component is movably nested at one end of the frame (1) close to the sealing cover (101). The culture component includes a dish body (103) and a convex block (104). The dish body (103) is the main body of the culture dish, and the convex block (104) is arranged in a semi-spherical shape; A groove is opened at the upper end of the frame (1) close to the sealing cover (101). There are multiple grooves. The convex block (104) is movably nested with the groove. The groove is vertically penetrated with the inside of the tray (102). The dish body (103) is movably nested with the frame (1) through the convex block (104), and a limiting component for further limiting the dish body (103) is arranged inside the groove of the frame (1); The tray (102) has a concave shape inside, and the concave edge of the tray (102) at the lower end of the frame (1) is movably nested; The dish body (103) is provided in a set with the convex block (104). The dish body (103) is concave. A cell patch is placed inside the dish body (103). Grooves are opened on the outer side of the upper end of the dish body (103), and 3-4 groups of grooves are vertically arranged; The convex block (104) has holes opened at its lower end and both sides. The inside of the convex block (104) is hollow. The number of the convex blocks (104) and the dish body (103) is matched with the number of grooves of the frame (1); The limiting component includes a snap ring (2), a rubber body (201), a pipe (202), a rotating sphere (3), an inclined rod (301) and a clamping block (302). The snap ring (2) is arranged inside the frame (1). The rubber body (201) is embedded in the inner wall of the snap ring (2). The pipe (202) penetrates through the inside of the snap ring (2). The rotating sphere (3) is hinged to the inner wall of one end of the pipe (202) away from the rubber body (201). The inclined rod (301) swings at one end of the rotating sphere (3). The clamping block (302) slides at one end of the inclined rod (301).

2. The cell culture dish for crayfish hemolymph experiments according to claim 1, characterized in that: The snap ring (2) is vertically sleeved with the upper groove of the frame (1). The convex block (104) is embedded inside the snap ring (2) through the upper groove of the frame (1). The inside of the snap ring (2) is hollow. The snap ring (2) is arranged in a circular ring shape.

3. The cell culture dish for crayfish hemolymph experiments according to claim 1, characterized in that: The inside of the rubber body (201) is filled with air. The rubber body (201) is integrally spherical. The inner wall thickness of the rubber body (201) is less than 0.4 cm. The side of the rubber body (201) away from the inner wall of the snap ring (2) is vertically pressed against the convex block (104). The rubber body (201) is arranged in a cross shape when viewed from above on the inner wall of the snap ring (2); The pipe (202) is arranged in a half-moon shape, that is, half of the moon shape. One end of the pipe (202) far from the rubber body (201) extends to the upper end of the inner wall of the snap ring (2). The pipe (202) and the rubber body (201) are provided in a matching manner.

4. The cell culture dish for crayfish hemolymph experiment according to claim 1, characterized in that: The rotating sphere (3) and the inclined rod (301) are provided in a matching manner. The inclined rod (301) is inclined at 15 - 45°. One end of each inclined rod (301) is connected to the clamping block (302). The outer side of the clamping block (302) is slidably and fittingly nested with the inner wall of the pipe (202). One end of the clamping block (302) is slidably nested with the outer groove of the dish body (103). The width of the inclined rod (301) is greater than 1 cm.

5. The cell culture dish for crayfish hemolymph experiment according to claim 1, characterized in that: A flow guide frame (4) runs through the middle of the inside of the tray (102). Both ends of the flow guide frame (4) are provided with elbow pipes (401). One end of the elbow pipe (401) is provided with a through pipe (403). A disc (402) is wound around the outer side of the through pipe (403). A baffle (404) is inlaid on the inner wall of the upper end of the through pipe (403).

6. The cell culture dish for crayfish hemolymph experiment according to claim 5, characterized in that: The flow guide frame (4) is arranged in a concave shape and extends to both sides at the lower end of the frame (1). Warm water is filled in the inside of the flow guide frame (4). The flow guide frame (4) communicates with the through pipe (403) through the elbow pipe (401).

7. The cell culture dish for crayfish hemolymph experiment according to claim 5, characterized in that: The elbow pipe (401) is arranged in an "S" shape. The elbow pipe (401) and the through pipe (403) are provided in a matching number with the convex blocks (104). The through pipe (403) extends to the upper end of the disc (402). The upper end of the through pipe (403) is vertically butted with the hole at the lower end of the convex block (104).

8. The cell culture dish for crayfish hemolymph experiment according to claim 5, characterized in that: The disc (402) is arranged in an annular shape. One end of the elbow pipe (401) penetrates into the inside of the disc (402). The disc (402) and the through pipe (403) are provided in a matching manner; The baffle (404), a plurality of baffles (404) are wound around the inner wall of the through pipe (403). The whole baffle (404) is arranged in an inverted "V" shape. The thickness of the baffle (404) is 0.1 - 0.15 cm.

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