Biomechanical testing system and reactor module therefor

Abstract

A biodynamic testing system and a reactor module thereof, the biodynamic testing system comprising a reactor module, a storage tank containing liquid and connected to the reactor module, and a gas pressure source connected to the storage tank. The reactor module includes an upper mold plate, a lower mold plate, a positioning plate disposed between the upper mold plate and the lower mold plate, a spacer, and at least one biological culture material. The upper mold plate, the lower mold plate, and the positioning plate cooperatively define a closed space for accommodating the spacer and the at least one biological culture material. The gas pressure source is controllable to provide gas to the storage tank, so that the liquid in the storage tank is forced by gas pressure into the closed space and flows through the at least one biological culture material to simulate the conditions of fluid pressure, fluid flow, and pulse in a living organism.

Description

Technical Field

[0001] This invention relates to a biomechanical testing system and its reactor module, and more particularly to a biomechanical testing system and its reactor module that simulates fluid-related environments in physiology for biomechanical testing (such as the circulatory system and the renal excretory system). Background Technology

[0002] Blood vessels in the human or animal body are intricately interconnected, bifurcating at different angles and diameters depending on their location and connections. In the August 2018 issue of the journal *Neurosurgery*, scholar Tetsuo Sasaki used computational fluid dynamics (CFD) to simulate blood vessel bifurcation models and elucidate how the geometry of bifurcation sites influences aneurysm formation. However, purely theoretical calculations still lack accuracy and struggle to rapidly simulate diverse biological environments. This issue also exists in other types of biomechanical testing besides blood vessels, indicating room for improvement. Summary of the Invention

[0003] The purpose of this invention is to provide a biomechanical testing system that can practically simulate the fluid-related environment in physiology.

[0004] The present invention discloses a biomechanical testing system comprising a reactor module, a storage tank containing liquid and connected to the reactor module, and a pressure source connected to the storage tank. The reactor module includes an upper template, a lower template disposed below the upper template, a positioning plate disposed between the upper and lower templates, a limiting member surrounded by the positioning plate and located between the upper and lower templates, and at least one biological culture material disposed corresponding to the limiting member. The upper template, the lower template, and the positioning plate cooperate to define a sealed space for accommodating the limiting member and the at least one biological culture material. The storage tank can be controlled to supply liquid to the sealed space, and the pressure source can be controlled to supply gas to the storage tank, thereby causing the liquid in the storage tank to enter the sealed space and flow through the at least one biological culture material through air pressure.

[0005] Preferably, in the aforementioned biomechanical testing system, the limiting member defines a flow channel corresponding to the at least one biological culture material and communicating with the sealed space, the flow channel through which liquid in the storage tank can flow.

[0006] Preferably, the aforementioned biomechanical testing system further includes a buffer tank connected to the reactor module, the buffer tank receiving excess liquid overflowing from the flow channel.

[0007] Preferably, the aforementioned biomechanical testing system defines a first horizontal direction, a second horizontal direction, and a vertical direction that are perpendicular to each other, wherein the limiting member has a plate portion extending along the first horizontal direction and two side edges protruding from the plate portion along the vertical direction, the side edges being spaced apart from each other along the second horizontal direction and each extending along the first horizontal direction.

[0008] Preferably, in the aforementioned biomechanical testing system, the limiting member further has several convex wall portions that extend toward each other from the side edge portion along the second horizontal direction.

[0009] Preferably, in the aforementioned biomechanical testing system, the limiting member further comprises at least one module portion disposed on the plate portion and located in the flow channel, the module portion being a polygonal column.

[0010] Preferably, in the aforementioned biomechanical testing system, the liquid in the storage tank enters the sealed space through the positioning plate, and the flow channel communicates with the sealed space. Excess liquid overflowing from the flow channel is discharged to the buffer tank through the upper template.

[0011] Preferably, the aforementioned biomechanical testing system further includes a drain pipe connected to the reactor module and, when opened, allows liquid to be discharged from the flow channel.

[0012] Preferably, the aforementioned biomechanical testing system further includes a circulation pipeline connecting the storage tank and the reactor module, and at least one pump disposed on the circulation pipeline.

[0013] Preferably, in the aforementioned biomechanical testing system, at least one biological culture material of the reactor module is a tubular biological culture material positioned by the limiting member, the biological culture material dividing the enclosed space into a flow channel and an outer ring groove surrounding the flow channel, the storage tank including a tank body containing liquid and connected to the gas pressure source, a first pipe connected to the tank body and communicating with the flow channel, and a second pipe connected to the tank body and communicating with the outer ring groove.

[0014] Preferably, in the aforementioned biomechanical testing system, the biomechanical testing system further includes a drain pipe connected to the reactor module and, when opened, allows liquid to be discharged from the flow channel. The limiting member has two receiving parts inserted into the positioning plate and positioning the biological culture material. The first pipe is connected to one of the receiving parts through the positioning plate, and the drain pipe is connected to the other receiving part through the positioning plate. The outer annular groove is also connected to the drain pipe.

[0015] Preferably, the aforementioned biomechanical testing system further includes two circulation pipelines that connect the storage tank and the drainage pipeline and can be controlled to open, one circulation pipeline connecting the flow channel and the other circulation pipeline connecting the outer annular groove and the second pipeline, each circulation pipeline being equipped with a pump.

[0016] Preferably, the aforementioned biomechanical testing system further includes a gas supply source that can controllably supply gas to the reactor module.

[0017] Preferably, in the aforementioned biomechanical testing system, the upper template has two downwardly extending electrode groups placed in the enclosed space, each in the shape of a comb, the electrode groups pressing, inserting, or passing through the at least one biological culture material.

[0018] Preferably, in the aforementioned biomechanical testing system, the lower template can heat the enclosed space.

[0019] Preferably, the aforementioned biomechanical testing system further includes two annular sealing gaskets sandwiched between the upper template and the positioning plate, and between the positioning plate and the lower template.

[0020] Another object of the present invention is to provide a reactor module in the biomechanical testing system.

[0021] The reactor module of the present invention includes an upper template, a lower template disposed below the upper template, a positioning plate disposed between the upper template and the lower template, a limiting member, and at least one biological culture material disposed corresponding to the limiting member. The positioning plate, the upper template, and the lower template cooperate to define a sealed space. The limiting member and the at least one biological culture material are disposed within the sealed space, surrounded by the positioning plate, and located between the upper template and the lower template.

[0022] Preferably, the aforementioned reactor module defines a first horizontal direction, a second horizontal direction, and a vertical direction that are perpendicular to each other, wherein the limiting member defines a flow channel communicating with the sealed space and includes a plate portion extending along the first horizontal direction and two side edges protruding from the plate portion along the vertical direction, the side edges being spaced apart from each other along the second horizontal direction and each extending along the first horizontal direction.

[0023] Preferably, in the aforementioned reactor module, the limiting member further includes several convex wall portions that extend toward each other from the side edge portion along the second horizontal direction.

[0024] Preferably, in the aforementioned reactor module, the limiting member further includes at least one module portion disposed on the plate portion and located in the flow channel, the module portion being a polygonal column.

[0025] Preferably, the aforementioned reactor module further includes a tubular biological culture material positioned by the limiting member, the biological culture material dividing the enclosed space into a flow channel and an outer annular groove surrounding the flow channel.

[0026] Preferably, in the aforementioned reactor module, the upper template has two downwardly extending electrode groups placed in the enclosed space, each in the shape of a comb, the electrode groups pressing, inserting, or passing through the at least one biological culture material.

[0027] Preferably, in the aforementioned reactor module, the lower template can heat the enclosed space.

[0028] The beneficial effects of this invention are as follows: the liquid contained in the storage tank is usually a culture medium, which, after being pressurized by the air pressure source, enters the sealed space and flows through the at least one biological culture material, thus simulating the flow of fluid within a living organism. In addition, the limiting member can be replaced with different types to simulate different biological environments and detect the effects of fluid pressure, fluid shear force, or fluid pulse on the cells on the at least one biological culture material. It has high versatility, and the sealed space formed within the reactor module can avoid external interference and further improve the accuracy of the simulation. Attached Figure Description

[0029] Figure 1 This is a schematic diagram illustrating the first embodiment of the biomechanical testing system of the present invention;

[0030] Figure 2 This is an exploded three-dimensional view illustrating the reactor module of the first embodiment;

[0031] Figure 3 This is a perspective view illustrating the electrode assembly of the first embodiment;

[0032] Figure 4 This is a side sectional view, illustrating... Figure 2 The assembled side view profile;

[0033] Figure 5 This is a frontal sectional view, illustrating... Figure 4 The frontal cross-sectional view;

[0034] Figures 6 to 11 All are three-dimensional diagrams illustrating the different states of the limiting components of the reactor module;

[0035] Figure 12This is a side sectional view illustrating another form and arrangement of the limiting member;

[0036] Figure 13 It's a 3D diagram, used for supplementary explanation. Figure 12 The three-dimensional pattern;

[0037] Figure 14 and Figure 15 These are all schematic diagrams illustrating different simulated actions in the first embodiment;

[0038] Figure 16 This is a schematic diagram illustrating another state of the first embodiment and its simulated action;

[0039] Figure 17 This is a front sectional view illustrating a second embodiment of the biomechanical testing system of the present invention;

[0040] Figure 18 This is a side sectional view, illustrating... Figure 17 The side view cross-sectional pattern;

[0041] Figure 19 This is a schematic diagram illustrating the configuration method of the second embodiment; and

[0042] Figures 20 to 23 All are schematic diagrams illustrating different simulated actions in the second embodiment. Detailed Implementation

[0043] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0044] See Figure 1 The first embodiment of the biomechanical testing system of the present invention includes a reactor module 1, a storage tank 2 containing liquid and connected to the reactor module 1, a gas pressure source 3 connected to the storage tank 2, a gas supply source 4 connected to the reactor module 1, a buffer tank 5 connected to the reactor module 1, and a drain pipe 6 connected to the reactor module 1.

[0045] See Figures 2 to 5 Define a first horizontal direction A, a second horizontal direction B, and a vertical direction C that are perpendicular to each other. The reactor module 1 includes a connection to the buffer tank 5 (see...). Figure 1 The upper template 11, and a drain pipe 6 (see) located below the upper template 11 along the vertical direction C and connected to the drain pipe 6. Figure 1The system comprises a lower template 12, a positioning plate 13 disposed between the upper template 11 and the lower template 12 along the vertical direction C, two sealing gaskets 14 respectively located between the upper template 11, the lower template 12 and the positioning plate 13 along the vertical direction C, a limiting member 15 surrounded by the positioning plate 13 and located between the upper template 11 and the lower template 12 along the vertical direction C, and a biological culture material 16 abutting against the limiting member 15. The upper template 11, the lower template 12 and the positioning plate 13 cooperate to define a sealed space 17 for accommodating the limiting member 15 and the biological culture material 16. The sealing gaskets 14 are ring-shaped and surround the sealed space 17.

[0046] The upper template 11 has two electrode groups 111 that are spaced apart from each other along the second horizontal direction B and each extends downward along the vertical direction C into the sealed space 17. Each electrode group 111 is as follows: Figure 3 The electrode arrangement is generally in the shape of a comb, meaning that each electrode group 111 has multiple electrodes spaced apart along the first horizontal direction A. The limiting member 15 has several slots on both sides for the electrode groups 111 to pass through (see...). Figure 2 This allows it to pass through the limiting member 15 and contact the biological culture material 16, thereby... Figure 5 The electrodes are pressed, inserted, or passed through the biological culture material 16 as shown, and the cells on the biological culture material 16 are electrically stimulated by an external power supply (e.g., for muscle cells, heart cells, or nerve cells). The comb-like shape and the configuration of inserting the electrodes into the biological culture material 16 can make the electrical stimulation more uniform and rapid. It should be noted that the electrode group 111 is a selectively installed component. Other types of electrodes can also be installed in this first embodiment, or no electrodes can be installed. Therefore, it should not be limited to this.

[0047] The lower template 12 is recessed along the vertical direction C to form a receiving groove 121 that connects to the sealed space 17. The receiving groove 121 is used to connect with the drainage pipe 6. Furthermore, the lower template 12 may also have a heating function, thereby heating the sealed space 17 to a set temperature to simulate the temperature inside the human body. The sealing gasket 14 is made of silicone and is used to ensure the airtightness of the sealed space 17. The upper template 11, the positioning plate 13, the lower template 12, and the sealing gasket 14 are fixed together in an overlapping manner by screws.

[0048] See Figure 2 , Figure 5 ,and Figure 6The limiting member 15 has a plate portion 151 extending along the first horizontal direction A, two side edge portions 152 protruding from the plate portion 151 along the vertical direction C, and two convex wall portions 153 protruding from the side edge portions 152 towards each other along the second horizontal direction B. The side edge portions 152 are spaced apart from each other along the second horizontal direction B and each extends along the first horizontal direction A. The plate portion 151, the side edge portions 152, and the convex wall portions 153 cooperate to define a flow channel 154 extending along the first horizontal direction A. The front and rear ends of the flow channel 154 are connected to the sealed space 17, and the side of the biological culture material 16 used for culturing cells faces the flow channel 154. It should be noted that when the limiting member 15 is placed in the sealed space 17, the side edge portions 152 are positioned downwards. However, for the sake of explaining the structure of the limiting member 15, Figure 6 It is presented upside down. The bioculture material 16 is located below the limiting member 15, close to the side edge 152. In this first embodiment, the bioculture material 16 may be a silicone film for culturing cells by adhesion, a glass slide for culturing cells and exomatrix, or a biomaterial suitable for cells and soft materials, and is not limited thereto.

[0049] See Figure 6 , Figure 7 ,and Figure 8 In this first embodiment, the convex wall portions 153 are each semi-circular and opposite to each other, making the flow channel 154 narrow, to simulate blood vessel blockage or other narrow environments in organisms. To simulate different degrees of blockage, the convex wall portions 153 can be adjusted as needed. Figures 6 to 8 The designs shown are of different sizes. (See attached image.) Figure 9 , Figure 10 ,and Figure 11 Alternatively, the limiting member 15 may not have the protruding wall portion 153, but instead has a module portion 155 that protrudes along the vertical direction C onto the plate portion 151 and is located in the flow channel 154. The module portion 155 can form a bifurcation point on the flow channel 154, thereby simulating a blood vessel bifurcation point or other biological environment. The module portion 155 can be shaped like a triangular prism, square prism, or other polygonal prism, and its protruding angles for generating the bifurcation can also be... Figures 9 to 11 The diagram shows different angles to simulate different types of blood vessel bifurcation points. Therefore, operators can replace the appropriate limiting component 15 according to the biological environment to be simulated, so as to create flow channels 154 with different boundary conditions as needed. It is highly versatile and conducive to modularization. In this first embodiment, the material of the module part 155 can be acrylic.

[0050] See Figure 12 and Figure 13 The limiting member 15 can also be configured in the opposite direction (so that the electrode assembly 111 does not need to pass through the limiting member 15), if the biological culture material 16 is adopted Figure 2 In the planar structure design, the biological culture material 16 is placed in reverse, with its top and bottom facing each other. When the aforementioned biological culture material 16 is as follows... Figure 12 When the material shown is a plurality of block-shaped biomedical materials, the limiting member 15 may be provided with several modular portions 155 along the first horizontal direction A, and each modular portion 155 is columnar, thus enabling... Figure 12 The biological culture material 16 is fixed to the limiting member 15 at intervals along the first horizontal direction A, thereby expanding the applicability of the biological culture material 16 and increasing the diversity of simulated detection.

[0051] See also Figure 1 , Figure 2 ,and Figure 4 The storage tank 2 contains culture medium and other liquids, and can be forced by the gas input from the pressure source 3 to allow the liquid to pass through the controlled opening silicone tube, through the positioning plate 13, into the sealed space 17, and into the flow channel 154. The gas supply source 4 is connected to the silicone tube of the storage tank 2 through a silicone tube, thereby connecting the sealed space 17. The buffer tank 5 is connected to the upper template 11 through a silicone tube. When there is too much liquid in the sealed space 17 or the flow channel 154, the excess liquid will overflow directly into the buffer tank 5. The drain pipe 6 is connected to the lower template 12 and connects to the receiving tank 121. It is also connected to the pipe of the buffer tank 5 and connects to the upper template 11. Both of these can be opened by control to drain the liquid to the outside for recycling (detailed steps of this part will be described later). It should be noted that although the storage tank 2, the air supply source 4, the buffer tank 5, and the drain pipe 6 are all connected to the sealed space 17, the storage tank 2, the air supply source 4, and the drain pipe 6 are all equipped with solenoid valves, and the buffer tank 5 is a closed environment, thus maintaining the sealing of the sealed space 17.

[0052] See Figure 1 , Figure 2 ,and Figure 6This first embodiment has multiple usage methods: When the culture medium is to be filled into the flow channel 154 or replaced for simulation, the main controller D controls the programmable gas pressure controller through an analog signal to open the solenoid valve and drive the gas pressure source 3 to send gas into the storage tank 2. The gas pressure can send the culture medium in the storage tank 2 into the sealed space 17 (the solenoid valve E on the pipeline of the storage tank 2 will be opened), and inject it into the flow channel 154 until it is full, in conjunction with the convex wall portion 153 (see Figure 6-8 ) or the module 155 (see Figure 9-11 The configuration allows for the creation of different flow channel environments (such as vascular blockage or bifurcation), enabling simulations of cyclic fluid shear, stable fluid pressure, and uniform and rapid electrical stimulation under these diverse biological structures. During the aforementioned filling or changing process, the solenoid valve F connecting the drain pipe 6 to the buffer tank 5 can be opened, allowing the culture medium to be discharged through the drain pipe 6. (See also...) Figure 1 , Figure 5 ,and Figure 14 The air pressure source 3 can also input the culture medium in the storage tank 2 into the flow channel 154 in a pulse manner to achieve the effect of fluid pulse input. The pulse situation can be checked by instruments such as pressure gauge G. In this way, fluid pulse stimulation simulation can be performed. During the pulse input process, the culture medium that overflows temporarily due to the pulse will first flow into the buffer tank 5 for temporary buffering. However, the buffer tank 5 can also be omitted, and the pressure can be left in the limiting member 15 for compression.

[0053] See Figure 1 , Figure 5 ,and Figure 15 When it is necessary to drain the liquid from the sealed space 17 and the flow channel 154, the air pressure source 3 can be turned off and the air supply source 4 can be turned on, allowing gas to directly enter the sealed space 17. The gas pressure will then discharge the culture medium through the drain pipe 6 (at this time, the solenoid valve H of the drain pipe 6 needs to be turned on), thus achieving the effect of draining the liquid. See also... Figure 1 , Figure 5 ,and Figure 16 The biomechanical testing system may also include a circulation pipeline 71 connecting the storage tank 2 and the reactor module 1 and equipped with a solenoid valve I, and a pump 72 installed on the circulation pipeline 71. This configuration allows the culture medium to be pumped out of the circulation pipeline 71 by the pump 72 after the flow channel 154 is filled (the solenoid valve I of the circulation pipeline 71 is opened), so that the culture medium flows back to the storage tank 2 to create a fluid shear cycle, achieving diverse conditions and environmental simulations.

[0054] See Figure 17 , Figure 18 ,and Figure 19 This is a second embodiment of the biomechanical testing system of the present invention. The second embodiment is largely the same as the first embodiment, except that the limiting member 15 of the reactor module 1 has two receiving portions 156 inserted into the positioning plate 13. The biological culture material 16 is tubular, dividing the sealed space 17 into an inner flow channel 154 and an outer ring groove 157 surrounding the flow channel 154. Both ends of the biological culture material 16 are respectively disposed on the receiving portions 156. The storage tank 2 includes a tank body 21 containing liquid and connected to the gas pressure source 3, a first pipe 22 connecting the tank body 21 and the flow channel 154 through the positioning plate 13, and a second pipe 23 connecting the tank body 21 and communicating with the outer ring groove 157. The first pipe 22 is connected to one of the receiving portions 156 through the positioning plate 13, and the drain pipe 6 is connected to the other receiving portion 156 through the positioning plate 13, and is also connected to the outer ring groove 157. The second embodiment does not include the buffer groove 5.

[0055] The operation of the second embodiment is as follows: The pressure source 3 provides gas to the tank 21, so as to send the culture medium in the tank 21 out through the first pipeline 22 (the solenoid valve J of the second pipeline 23 is closed) by the pressure, thereby allowing the culture medium to enter the flow channel 154. In addition, the culture medium can also be discharged by opening the solenoid valve K of the drain pipeline 6. When a pulse reaction is desired, only the solenoid valve L of the first pipeline 22 can be opened, and the main controller D controls the programmable pressure controller via analog signal to intermittently input gas from the pressure source 3 to generate a pulse reaction in the flow channel 154. See reference. Figure 18 and Figure 20 The user can also close the first pipe 22 and open the second pipe 23, so that the culture medium can enter the outer ring tank 157 through the second pipe 23, and the culture medium can be discharged by opening another solenoid valve M of the drainage pipe 6, thus achieving another environmental simulation effect.

[0056] See Figure 18 and Figure 21 This second embodiment also includes two circulation pipes 71 that connect the storage tank 2 and the drainage pipe 6 and can be controlled to open. One circulation pipe 71 connects to the flow channel 154, and the other circulation pipe 71 connects to the outer annular groove 157 and the second pipe 23. Each circulation pipe 71 is equipped with a pump 72. When the circulation pipe 71 connecting to the flow channel 154 is opened, the culture medium can be drawn by the pump 72 of the circulation pipe 71 after entering the flow channel 154 (correspondingly, the solenoid valve N is opened) to return to the tank 21 through the circulation pipe 71, achieving a simulation effect of internal circulation. See reference. Figure 18 and Figure 22 When the outer ring tank 157 is filled with culture medium, most of the solenoid valves can be closed, leaving only the solenoid valve J of the second pipeline 23 and the solenoid valve O of the circulation pipeline 71 connected to the outer ring tank 157 open. The culture medium is then circulated between the second pipeline 23, the outer ring tank 157, the drainage pipeline 6 (which will not be discharged because the solenoid valve M is closed), and the circulation pipeline 71 by the pump 72, achieving the simulation effect of external circulation.

[0057] See also Figure 18 and Figure 23 When the culture medium in the flow channel 154 needs to be emptied, the gas pressure source 3 can be turned off and the gas supply source 4 can be turned on. This allows the gas from the gas supply source 4 to be directly sent into the flow channel 154 through the first pipe 22, so that the culture medium can be discharged through the drain pipe 6, achieving the effect of emptying the liquid in the pipe. If the culture medium in the outer ring tank 157 needs to be emptied, another gas supply source (not shown) connected to the second pipe 23 can be set up. In this second embodiment, tubular biomedical materials can be used as the biological culture material 16 to improve the diversity of the simulated environment.

[0058] In summary, the present invention allows for the replacement of different types of biological culture materials 16 as needed, and only the limiting member 15 needs to be replaced to create flow channels 154 with different structural features such as narrowness and branching. In addition, it can create various biological environment simulations such as circulating fluid shear force simulation, stable fluid pressure simulation, fluid pulse stimulation simulation, and electrical stimulation simulation, thereby achieving a highly versatile detection effect. Therefore, the present invention can indeed achieve its purpose.

Claims

1. A biomechanical testing system, characterized in that: The biomechanical testing system includes a reactor module, a storage tank containing liquid and connected to the reactor module, and a pressure source connected to the storage tank. The reactor module includes an upper template, a lower template disposed below the upper template, a positioning plate disposed between the upper and lower templates, a limiting member surrounded by the positioning plate and located between the upper and lower templates, and at least one biological culture material disposed corresponding to the limiting member. The upper template, the lower template, and the positioning plate cooperate to define a sealed space for accommodating the limiting member and the at least one biological culture material. The storage tank can be controlled to supply liquid to the sealed space, and the pressure source can be controlled to supply gas to the storage tank. The liquid in the storage tank is forced into the sealed space by air pressure and flows through the at least one biological culture material. A first horizontal direction, a second horizontal direction, and a vertical direction are defined that are perpendicular to each other. The limiting member defines a flow channel corresponding to the at least one biological culture material and communicating with the sealed space. The flow channel allows the liquid in the storage tank to flow through. The limiting member has a plate portion extending along the first horizontal direction, two side edges protruding from the plate portion along the vertical direction, and at least one module portion disposed on the plate portion and located in the flow channel. The side edges are spaced apart from each other along the second horizontal direction and each extends along the first horizontal direction. The module portion is a polygonal column.

2. The biomechanical testing system according to claim 1, characterized in that: The biomechanical testing system also includes a buffer tank connected to the reactor module, which receives excess liquid overflowing from the flow channel.

3. The biomechanical testing system according to claim 1, characterized in that: The limiting member also has several convex wall portions that protrude towards each other from the side edge portion along the second horizontal direction.

4. The biomechanical testing system according to claim 2, characterized in that: The limiting member also has several convex wall portions that protrude towards each other from the side edge portion along the second horizontal direction.

5. The biomechanical testing system according to claim 2 or 4, characterized in that: The liquid in the storage tank enters the sealed space through the positioning plate, and the flow channel connects to the sealed space. Excess liquid overflowing from the flow channel is discharged into the buffer tank through the upper template.

6. The biomechanical testing system according to claim 5, characterized in that: The biomechanical testing system also includes a drain pipe that connects to the reactor module and allows liquid to be discharged from the flow channel when the system is turned on.

7. The biomechanical testing system according to claim 6, characterized in that: The biomechanical testing system also includes a circulation pipeline connecting the storage tank and the reactor module, and at least one pump installed on the circulation pipeline.

8. The biomechanical testing system according to claim 1, characterized in that: At least one biological culture material of the reactor module is a tubular biological culture material positioned by the limiting member, the biological culture material dividing the enclosed space into a flow channel and an outer ring groove surrounding the flow channel, the storage tank includes a tank body containing liquid and connected to the gas pressure source, a first pipe connected to the tank body and in communication with the flow channel, and a second pipe connected to the tank body and in communication with the outer ring groove.

9. The biomechanical testing system according to claim 8, characterized in that: The biomechanical testing system also includes a drain pipe connected to the reactor module and which allows liquid to be discharged from the flow channel when opened. The limiting member has two receiving parts inserted into the positioning plate and positioning the biological culture material. The first pipe is connected to one of the receiving parts through the positioning plate, and the drain pipe is connected to the other receiving part through the positioning plate. The outer ring groove is also connected to the drain pipe.

10. The biomechanical testing system according to claim 9, characterized in that: The biomechanical testing system also includes two circulation pipelines that connect the storage tank and the drainage pipeline and can be controlled to open. One circulation pipeline is connected to the flow channel, and the other circulation pipeline is connected to the outer ring tank and the second pipeline. Each circulation pipeline is equipped with a pump.

11. The biomechanical testing system according to claim 1, characterized in that: The biomechanical testing system also includes a gas supply source that can controllably supply gas to the reactor module.

12. The biomechanical testing system according to claim 1, characterized in that: The upper template has two downward-extending electrode groups placed in the sealed space, each in the shape of a comb, which press, insert, or pass through at least one biological culture material.

13. The biomechanical testing system according to claim 1, characterized in that: The lower template can heat the enclosed space.

14. The biomechanical testing system according to claim 1, characterized in that: The biomechanical testing system also includes two ring-shaped sealing gaskets sandwiched between the upper template and the positioning plate, and between the positioning plate and the lower template.

15. A reactor module, characterized in that: The reactor module includes an upper template, a lower template disposed below the upper template, a positioning plate disposed between the upper template and the lower template, a limiting member, and at least one biological culture material disposed corresponding to the limiting member. The positioning plate, the upper template, and the lower template cooperate to define a sealed space. The limiting member and the at least one biological culture material are disposed within the sealed space, surrounded by the positioning plate, and located between the upper template and the lower template, defining a first horizontal direction, a second horizontal direction, and a vertical direction that are perpendicular to each other. The limiting member defines a flow channel communicating with the sealed space and includes a plate portion extending along the first horizontal direction, two side edges protruding from the plate portion along the vertical direction, and at least one module portion disposed on the plate portion and located in the flow channel. The side edges are spaced apart from each other along the second horizontal direction and each extends along the first horizontal direction. The module portion is a polygonal column.

16. The reactor module according to claim 15, characterized in that: The limiting member also includes several convex wall portions that protrude towards each other from the side edge portion along the second horizontal direction.

17. The reactor module according to claim 15, characterized in that: The reactor module also includes a tubular biological culture material positioned by the limiting member, which divides the enclosed space into a flow channel and an outer ring groove surrounding the flow channel.

18. The reactor module according to claim 15, characterized in that: The upper template has two downward-extending electrode groups placed in the sealed space, each in the shape of a comb, which press, insert, or pass through at least one biological culture material.

19. The reactor module according to claim 15, characterized in that: The lower template can heat the enclosed space.

Images