Biological tissue transparent processing device and processing method

Abstract

The present invention discloses a biological tissue transparent processing device and processing method, comprising a frame, a chip, a peristaltic pump, and a reactor. The interior of the frame is divided into an upper and lower layers. The lower layer of the frame is respectively provided with a liquid tank and a temperature-controlled heating module, and the upper layer of the frame is respectively provided with an adjustable power supply, a chip, a refrigeration module, a peristaltic pump, and a reactor. The chip is arranged above the adjustable power supply. After power is turned on, these negatively charged compounds can quickly separate from the tissue and move toward the positive electrode, thereby achieving the purpose of transparent tissue. At the same time, the attraction and separation mechanism will continuously rotate to adjust the attraction to the biological tissue. As the attraction and separation mechanism rotates, the reaction liquid will continuously enter the water inlet circulation mechanism. When the water flow enters the interior of the water inlet circulation mechanism, it will generate resistance to the reaction filtration mechanism, causing the reaction filtration mechanism to vibrate, thereby increasing the separation of lipids in the biological tissue.

Description

Technical Field

[0001] The present invention relates to the technical field of biological tissue transparent processing, in particular to a biological tissue transparent processing device and processing method. Background Art

[0002] Tissue clearing technology uses a mixture of organic compounds or ionic detergents to treat brain tissue, changing its optical properties from a strong scattering medium to a weak scattering medium, enhancing light transmittance, and thus increasing the imaging depth of optical microscopes. This is conducive to the realization of three-dimensional reconstruction of brain tissue and the analysis of the spatial structure of neurons. When clearing biological tissue, a clearing treatment device is required. However, general clearing treatment devices have some disadvantages when used, such as:

[0003] When in use, a general transparent processing device only uses a reaction liquid to continuously flush the biological tissue, thereby using the reaction liquid to react with the substances in the biological tissue to achieve the purpose of transparentizing the biological tissue. The general transparent processing device only uses a single physical method to increase the speed of transparent processing, making the transparent processing speed slow. It usually takes 3-5 days to complete the transparentization of the biological tissue, which is not conducive to the use of the device. Summary of the Invention

[0004] The object of the present invention is to provide a biological tissue transparent processing device and processing method to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: a biological tissue transparent processing device and processing method, comprising a frame, a chip, a peristaltic pump, and a reactor, the interior of the frame is divided into an upper and lower layers, the lower layer of the frame is respectively provided with a liquid tank and a temperature control heating module, the upper layer of the frame is respectively provided with an adjustable power supply, a chip, a refrigeration module, a peristaltic pump and a reactor, the chip is arranged above the adjustable power supply, the reactor is arranged at the inner center of the frame, and the upper end of the reactor is provided with a rotating cap structure, the rotating cap structure The lower end of the structure is provided with a reaction filtering mechanism, the interior of the reaction filtering mechanism is provided with a water inlet circulation mechanism, the lower end of the reactor is provided with a water inlet mechanism, the side of the rack is provided with an attraction and separation mechanism, the operation program of the equipment is stored in the chip, the reaction steps are controlled by the chip, the adjustable power supply can provide power for the device, the refrigeration module and the temperature control heating module can control the temperature of the reactor within the ideal temperature range, the liquid tank can store about 3L of liquid, the liquid is transported to the reactor by a peristaltic pump, and the liquid of the reactor is transported back to the liquid tank, when the reaction liquid enters the reactor from the water inlet mechanism After entering the reactor, it will drive the reaction filter mechanism to rotate, and at the same time, a slowly rising water flow will be generated on the inner side of the reaction filter mechanism. The reaction liquid can combine with the lipid substances in the biological tissue to form negatively charged compounds. After power is applied, these negatively charged compounds can quickly detach from the tissue and move toward the positive electrode, thereby achieving the purpose of transparent tissue. At the same time, the attraction and separation mechanism will continuously rotate to adjust the attraction of the biological tissue. As the attraction and separation mechanism rotates, the reaction liquid will continuously enter the water inlet circulation mechanism. When the water flow enters the interior of the water inlet circulation mechanism, it will generate resistance to the reaction filter mechanism. Since the force inside the reaction filter mechanism is different from the force outside, the reaction filter mechanism will vibrate, increasing the separation of lipids in the biological tissue. Afterwards, the reaction liquid will be ejected from the center of the reaction filter mechanism to form a high-speed water flow to provide lift for the biological tissue. However, due to the continuous vibration of the reaction filter mechanism, the reaction liquid entering the water inlet circulation mechanism is irregular, thereby making the direction of the water flow ejected from the center of the reaction filter mechanism uncertain, so that the high-speed water flow in the center can drive the biological tissue to rotate, increasing the transparency effect of the device.

[0006] Furthermore, the rotating cap structure includes a fixing cover, a locking rod, a connecting column and a fixing cap, the lower end of the fixing cover is provided with a rubber sleeve, the fixing cover is embedded in the interior of the reactor, the inner upper end of the fixing cover is slidably provided with a locking rod, the lower end of the fixing cover is rotatably provided with a connecting column, the lower end of the connecting column is provided with a fixing cap, the fixing cap is threadedly connected to the reaction filtering mechanism, and the locking rod and the connecting column are snap-fitted. When the device is in use, the biological tissue is placed inside the reaction filtering mechanism, and then the fixing cap and the reaction filtering mechanism are connected, the locking rod is moved downward to engage with the connecting column, and the fixing cover is rotated to connect the fixing cap and the reaction barrel, and a spring is provided inside the fixing cover. When the locking rod is not subjected to external force, the spring will push the locking rod to move upward, so that the locking rod will not limit the connecting column, so that the connecting column and the fixing cap can rotate under the action of external force.

[0007] Furthermore, the reaction filtration mechanism includes a reaction barrel and a filter layer. The reaction barrel is threadedly connected to the rotating cap structure. The interior of the reaction barrel is hollow, the lower end of the reaction barrel is arc-shaped, the side of the reaction barrel is provided with a filter layer, and the lower end side of the reaction barrel is provided with a baffle at equal angles. When the device is in use, the filter layer can prevent biological tissue from moving outward, and the lipid substances reacting with the reaction liquid will move outward with the reaction liquid through the filter layer, thereby gradually extracting the substances in the biological tissue, making the biological tissue transparent.

[0008] Furthermore, the water inlet circulation mechanism includes a water inlet pipe, a fixed frame and a drainage hole. The water inlet pipe is arranged at equal angles inside the reaction barrel, one end of the water inlet pipe is arranged on the side of the reaction barrel, and the water inlet pipe is located at the lower end of the filter layer, and the other end of the water inlet pipe is connected to the fixed frame. The fixed frame is cylindrical as a whole, and the upper end of the fixed frame is concave. Drain holes are evenly distributed inside the upper end of the fixed frame. There is a certain angle between the water inlet pipe and the reaction barrel. When the reaction liquid enters from the water inlet pipe, when the angle of the water inlet pipe is opposite to the angle of entry of the reaction liquid, the reverse thrust generated on the reaction barrel is the largest, and when the angle of the water inlet pipe is the same as the angle of entry of the reaction liquid, the thrust received by the reaction barrel is the smallest. Therefore, the angle of the water inlet pipe can be adjusted according to actual conditions, so that the reaction barrel can continue to rotate even when it is subjected to the impact force from the water inlet pipe during operation, thereby causing the reaction barrel to rotate continuously. Since the rotation angles of the reaction barrel and the attraction and separation mechanism are opposite, the transparency effect of the device will be increased.

[0009] Furthermore, the water inlet mechanism is embedded in the interior of the reaction filtration mechanism, and the water inlet mechanism includes a water inlet pipe and a fixed channel. The water inlet pipe is arranged at the bottom of the reactor, and an arc-shaped fixed channel is arranged at an equal angle inside the upper end of the water inlet pipe. The reaction liquid in the liquid tank will pass through the water inlet pipe to the interior of the reactor under the action of the peristaltic pump, and the reaction liquid will enter the interior of the fixed channel through the water inlet pipe. Since the fixed channel is arc-shaped, the reaction liquid will enter the interior of the reaction barrel with a certain arc, thereby generating a driving force on the baffle in the reaction barrel, causing the reaction barrel to rotate. Since the energy of the liquid ejected from the fixed channel will be consumed in the rotation of the reaction barrel, the reaction liquid will slowly rise in the reaction barrel, thereby avoiding the water flow from affecting the biological tissue in the middle.

[0010] Furthermore, a negative electrode rod is provided at the bottom of the reactor, and the attraction and separation mechanism includes a positive electrode rod, an attraction layer, a connecting rod, a fixed gear, a connecting gear and a motor. The positive electrode rod is provided on the side of the reactor, and an annular attraction layer is provided for rotation inside the reactor. The attraction layer and the positive electrode rod are fitted together, and the upper and lower ends of the attraction layer are respectively provided with connecting rods, and the side of the upper end of the connecting rod is provided with a fixed gear, and the fixed gear and the connecting gear are meshed and connected. The connecting gear is connected to the motor, and the motor is provided on the outside of the reactor. The motor will drive the connecting gear to rotate, and when the connecting gear is rotated When it rotates, it will drive the fixed gear to rotate. When the fixed gear rotates, it will drive the attraction layer to rotate through the connecting rod. The attraction layer is ring-shaped. The positive electrode rod will make the attraction layer carry a positive charge. There are several attraction areas inside the attraction layer. The positive charge in the attraction area is more than that in the attraction layer. When the attraction layer rotates, the position of the attraction area will also change, thereby adjusting the position of the positive charge, so that the attraction layer can more effectively attract the lipids of biological tissues. A negative electrode rod is set at the bottom center of the reactor. The negative electrode rod will push the lipid substances outward, and the reaction liquid will flow back into the liquid tank at the edge of the reactor.

[0011] Furthermore, a fixing ring is provided on the side of the connecting rod at the lower end, and an inclined guide layer is provided on the side of the fixing ring. When the connecting rod rotates, the fixing ring and the guide layer will be driven to rotate. When the guide layer moves, due to its inclined shape, the guide layer will drive the reaction liquid flow to move, thereby allowing the reaction liquid flow to enter the interior of the water inlet pipe. The inlet of the water inlet pipe is in the shape of a filter, which can prevent substances from flowing into the interior of the water inlet pipe, and the angle of the guide layer can be adaptively adjusted to control the speed at which the reaction liquid enters the water inlet pipe.

[0012] A method for transparentizing biological tissue, comprising:

[0013] a. Open the lid on the top of the rack, open the rotating cap structure of the reactor, place the tissue into the reactor, and lock the sealing cover tightly;

[0014] b. Turn on the power and start the machine automatically;

[0015] c. Add 1L of reaction liquid into the liquid tank. The peristaltic pump automatically draws the reaction liquid. At the same time, the temperature control heating module and the cooling module adjust the temperature of the reactor.

[0016] d. Use physical methods and electricity to accelerate the combination and shedding speed of the reaction solution and fat, achieving tissue transparency within 3-5 days;

[0017] e. Remove the transparent tissue from the reactor and clean the device.

[0018] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: when the present invention is in use, after the reaction liquid enters the reactor from the water inlet mechanism, it will push the reaction filtration mechanism to rotate, and at the same time, a slowly rising water flow will be generated on the inner side of the reaction filtration mechanism. The reaction liquid can combine with the lipid substances in the biological tissue to form negatively charged compounds. After power is turned on, these negatively charged compounds can quickly separate from the tissue and move toward the positive electrode, thereby achieving the purpose of transparent tissue. At the same time, the attraction and separation mechanism will continuously rotate to adjust the attraction to the biological tissue, and as the attraction and separation mechanism rotates, the reaction liquid will continuously enter the water inlet circulation mechanism, and the water When the flow enters the water inlet circulation mechanism, it will generate resistance to the reaction filtration mechanism. Since the force applied to the inside of the reaction filtration mechanism is different from the force applied to the outside, the reaction filtration mechanism will vibrate, increasing the detachment of lipids in the biological tissue. After that, the reaction liquid will be ejected from the center of the reaction filtration mechanism to form a high-speed water flow to provide lift for the biological tissue. Since the reaction filtration mechanism is constantly vibrating, the reaction liquid entering the water inlet circulation mechanism is irregular, which makes the direction of the water flow ejected from the center of the reaction filtration mechanism uncertain, so that the high-speed water flow in the center can drive the biological tissue to rotate, increasing the transparency of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0020] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the front cross-sectional structure of the reactor of the present invention;

[0022] Figure 3This is a schematic diagram of the exploded structure of the fixed cover and the reaction barrel of the present invention;

[0023] Figure 4 It is a schematic diagram of the front cross-sectional structure of the fixed cover of the present invention;

[0024] Figure 5 Schematic diagram of the top cross-sectional structure of the attraction layer and the fixed gear of the present invention;

[0025] Figure 6 It is a schematic diagram of the water flow direction structure in the reaction barrel;

[0026] Figure 7 It is a schematic diagram of the rotation direction of the reaction barrel and the attraction layer;

[0027] Figure 8 It is a schematic diagram of the rotation direction of the reaction barrel and the fixed ring.

[0028] In the figure: 1. Rack; 2. Liquid tank; 3. Temperature control heating module; 4. Adjustable power supply; 5. Chip; 6. Refrigeration module; 7. Peristaltic pump; 8. Reactor; 9. Fixed cover; 10. Locking rod; 11. Connecting column; 12. Fixed cap; 13. Reactor barrel; 14. Filter layer; 15. Water inlet pipe; 16. Fixed frame; 17. Drain hole; 18. Baffle; 19. Water inlet pipe; 20. Fixed channel; 21. Negative electrode rod; 22. Positive electrode rod; 23. Attraction layer; 24. Connecting rod; 25. Fixed gear; 26. Connecting gear; 27. Motor; 28. Fixed ring; 29. ​​Guide layer. DETAILED DESCRIPTION

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0030] like Figure 1 、 Figure 2 Figure 6 、 Figure 7 and Figure 8As shown, the present invention provides a technical solution: a biological tissue transparent processing device and processing method, including a frame 1, a chip 5, a peristaltic pump 7, and a reactor 8. The interior of the frame 1 is divided into an upper and lower layers. The lower layer of the frame 1 is respectively provided with a liquid tank 2 and a temperature control heating module 3. The upper layer of the frame 1 is respectively provided with an adjustable power supply 4, a chip 5, a refrigeration module 6, a peristaltic pump 7 and a reactor 8. The chip 5 is arranged above the adjustable power supply 4, and the reactor 8 is arranged at the center of the interior of the frame 1. The upper end of the interior of the reactor 8 is provided with a rotating cap structure, and the lower end of the rotating cap structure is provided with There is a reaction filtering mechanism, the interior of the reaction filtering mechanism is provided with a water inlet circulation mechanism, the lower end of the reactor 8 is provided with a water inlet mechanism, the side of the rack 1 is provided with an attraction and separation mechanism, the operation program of the equipment is stored in the chip 5, the reaction steps are controlled by the chip 5, the adjustable power supply 4 can provide power for the device, the refrigeration module 6 and the temperature control heating module 3 can control the temperature of the reactor within the ideal temperature range, the liquid tank 2 can store about 3L of liquid, the liquid is transported to the reactor 8 by the peristaltic pump 7, and the liquid of the reactor 8 is transported back to the liquid tank 2, when the reaction liquid enters the reactor from the water inlet mechanism After entering the reactor 8, the reaction filter mechanism is driven to rotate, and a slowly rising water flow is generated on the inner side of the reaction filter mechanism. The reaction liquid can combine with the lipid substances in the biological tissue to form negatively charged compounds. After power is applied, these negatively charged compounds can quickly separate from the tissue and move toward the positive electrode, thereby achieving the purpose of transparent tissue. At the same time, the attraction and separation mechanism will continuously rotate to adjust the attraction of the biological tissue. As the attraction and separation mechanism rotates, the reaction liquid will continuously enter the water inlet circulation mechanism. When the water flow enters the interior of the water inlet circulation mechanism, it will generate resistance to the reaction filter mechanism. Since the force inside the reaction filter mechanism is different from the force outside, the reaction filter mechanism will vibrate, increasing the separation of lipids in the biological tissue. Afterwards, the reaction liquid will be ejected from the center of the reaction filter mechanism to form a high-speed water flow to provide lift for the biological tissue. Since the reaction filter mechanism is constantly vibrating, the reaction liquid entering the water inlet circulation mechanism is irregular, resulting in an uncertain direction of the water flow ejected from the center of the reaction filter mechanism. The high-speed water flow in the center can drive the biological tissue to rotate, thereby increasing the transparency effect of the device.

[0031] like Figure 3As shown, the rotating cap structure includes a fixed cover 9, a locking rod 10, a connecting column 11 and a fixed cap 12. The lower end of the fixed cover 9 is provided with a rubber sleeve, and the fixed cover 9 is embedded in the interior of the reactor 8. The upper end of the interior of the fixed cover 9 is slidably provided with a locking rod 10, and the lower end of the fixed cover 9 is rotatably provided with a connecting column 11. The lower end of the connecting column 11 is provided with a fixing cap 12, and the fixing cap 12 is threadedly connected to the reaction filtration mechanism. The locking rod 10 and the connecting column 11 are snap-fitted. When the device is in use, the biological tissue is placed inside the reaction filtration mechanism, and then the fixing cap 12 and the reaction filtration mechanism are connected. The locking rod 10 is moved downward to engage with the connecting column 11, and the fixed cover 9 is rotated to connect the fixing cap 12 and the reaction barrel 13. A spring is provided inside the fixed cover 9. When the locking rod 10 is not subjected to external force, the spring will push the locking rod 10 to move upward, so that the locking rod 10 will not limit the connecting column 11, so that the connecting column 11 and the fixing cap 12 can rotate under the action of external force.

[0032] like Figure 4 As shown, the reaction filtration mechanism includes a reaction barrel 13 and a filter layer 14. The reaction barrel 13 is threadedly connected to the rotating cap structure. The interior of the reaction barrel 13 is hollow, and the lower end of the reaction barrel 13 is arc-shaped. The side of the reaction barrel 13 is provided with a filter layer 14, and the lower end side of the reaction barrel 13 is provided with a baffle 18 at equal angles. When the device is in use, the filter layer 14 can prevent the biological tissue from moving outward, and the lipid substances reacting with the reaction liquid will move outward with the reaction liquid through the filter layer 14, thereby gradually extracting the substances in the biological tissue, making the biological tissue transparent.

[0033] The water inlet circulation mechanism includes a water inlet pipe 15, a fixed frame 16 and a drainage hole 17. The water inlet pipe 15 is arranged at an equal angle inside the reaction barrel 13. One end of the water inlet pipe 15 is arranged on the side of the reaction barrel 13, and the water inlet pipe 15 is located at the lower end of the filter layer 14. The other end of the water inlet pipe 15 is connected to the fixed frame 16. The fixed frame 16 is cylindrical as a whole, and the upper end of the fixed frame 16 is concave. The drainage holes 17 are evenly distributed inside the upper end of the fixed frame 16. There is a certain angle between the water inlet pipe 15 and the reaction barrel 13. When the reaction liquid enters from the water inlet pipe 15, When the angle of the water inlet pipe 15 is opposite to the angle at which the reaction liquid enters, the reverse thrust generated on the reaction barrel 13 is the largest, and when the angle of the water inlet pipe 15 is the same as the angle at which the reaction liquid enters, the thrust on the reaction barrel 13 is the smallest. Therefore, the angle of the water inlet pipe 15 can be adjusted according to actual conditions, so that the reaction barrel 13 can continue to rotate despite the impact force from the water inlet pipe 15 during operation, thereby causing the reaction barrel 13 to rotate continuously. Since the rotation angles of the reaction barrel 13 and the suction and separation mechanism are opposite, the transparency effect of the device is increased.

[0034] The water inlet mechanism is embedded in the interior of the reaction filtration mechanism. The water inlet mechanism includes a water inlet pipe 19 and a fixed channel 20. The water inlet pipe 19 is arranged at the bottom of the reactor 8, and an arc-shaped fixed channel 20 is arranged at an equal angle inside the upper end of the water inlet pipe 19. The reaction liquid in the liquid tank 2 will pass through the water inlet pipe 19 to the interior of the reactor 8 under the action of the peristaltic pump 7. The reaction liquid will enter the interior of the fixed channel 20 through the water inlet pipe 19. Since the fixed channel 20 is arc-shaped, the reaction liquid will enter the interior of the reaction barrel 13 with a certain arc, thereby generating a driving force for the baffle 18 in the reaction barrel 13, causing the reaction barrel 13 to rotate. Since the liquid energy ejected from the fixed channel 20 will be consumed in the rotation of the reaction barrel 13, the reaction liquid will slowly rise in the reaction barrel 13, thereby avoiding the water flow from affecting the biological tissue in the middle.

[0035] A negative electrode rod 21 is provided at the bottom of the reactor 8, and the attraction and separation mechanism includes a positive electrode rod 22, an attraction layer 23, a connecting rod 24, a fixed gear 25, a connecting gear 26 and a motor 27. The positive electrode rod 22 is provided on the side of the reactor 8, and the internal rotation of the reactor 8 is provided with a ring-shaped attraction layer 23. The attraction layer 23 and the positive electrode rod 22 are fitted together. The upper and lower ends of the attraction layer 23 are respectively provided with connecting rods 24, and the side of the upper end connecting rod 24 is provided with a fixed gear 25. The fixed gear 25 and the connecting gear 26 are meshed and connected. The connecting gear 26 is connected to the motor 27. The motor 27 is provided on the outside of the reactor 8. The motor 27 will drive the connecting gear 26 to rotate. When the connecting gear 26 rotates When the fixed gear 25 rotates, it will drive the fixed gear 25 to rotate. When the fixed gear 25 rotates, it will drive the attraction layer 23 to rotate through the connecting rod 24. The attraction layer 23 is annular. The positive rod 22 will make the attraction layer 23 have a positive charge, and there are several attraction areas inside the attraction layer 23. The positive charge in the attraction area is more than that in the attraction layer 23. When the attraction layer 23 rotates, the position of the attraction area will also change, thereby adjusting the position of the positive charge, so that the attraction layer 23 can more effectively attract the lipids of biological tissues, and a negative rod 21 is provided at the bottom center of the reactor 8. The negative rod 21 will push the lipid substances to move outward, and the reaction liquid will flow back into the interior of the liquid tank 2 at the edge of the reactor 8.

[0036] like Figure 5As shown, a fixing ring 28 is provided on the side of the connecting rod 24 at the lower end, and an inclined guide layer 29 is provided on the side of the fixing ring 28. When the connecting rod 24 rotates, the fixing ring 28 and the guide layer 29 are driven to rotate. When the guide layer 29 moves, due to its inclined shape, the guide layer 29 drives the reaction liquid flow to move, thereby allowing the reaction liquid flow to enter the interior of the water inlet pipe 15. The inlet of the water inlet pipe 15 is in the shape of a filter, which can prevent substances from flowing into the interior of the water inlet pipe 15, and the angle of the guide layer 29 can be adaptively adjusted to control the speed at which the reaction liquid enters the water inlet pipe 15.

[0037] A method for transparentizing biological tissue, comprising:

[0038] a. Open the lid on the top of the rack 1, open the rotating cap structure of the reactor 8, place the tissue into the reactor 8, and lock the sealing cover tightly;

[0039] b. Turn on the power and start the machine automatically;

[0040] c. Add 1L of reaction liquid to the liquid tank 2, and the peristaltic pump 7 automatically sucks the reaction liquid. At the same time, the temperature control heating module 3 and the refrigeration module 6 will adjust the temperature of the reactor 8;

[0041] d. Use physical methods and electricity to accelerate the combination and shedding speed of the reaction solution and fat, achieving tissue transparency within 3-5 days;

[0042] e. The transparent tissue after the reaction is completed is removed from the reactor 8 and the device is cleaned.

[0043] The working principle of the present invention is as follows: when the device is in use, the reaction liquid enters the reactor 8 from the water inlet mechanism, which will push the reaction filter mechanism to rotate, and at the same time, a slowly rising water flow will be generated on the inner side of the reaction filter mechanism. The reaction liquid can combine with the lipid substances in the biological tissue to form negatively charged compounds. After power is turned on, these negatively charged compounds can quickly separate from the tissue and move toward the positive electrode, thereby achieving the purpose of transparent tissue. At the same time, the attraction and separation mechanism will continue to rotate to adjust the attraction to the biological tissue, and as the attraction and separation mechanism rotates, the reaction liquid will continue to enter the water inlet circulation mechanism, and the water flow enters the water inlet circulation mechanism. The inside of the circulation mechanism will generate resistance to the reaction filtration mechanism. Since the force applied to the inside of the reaction filtration mechanism is different from the force applied to the outside, the reaction filtration mechanism will vibrate, increasing the detachment of lipids in the biological tissue. After that, the reaction liquid will be ejected from the center of the reaction filtration mechanism to form a high-speed water flow to provide lift for the biological tissue. Since the reaction filtration mechanism is constantly vibrating, the reaction liquid entering the water inlet circulation mechanism is irregular, which makes the direction of the water flow ejected from the center of the reaction filtration mechanism uncertain, so that the high-speed water flow in the center can drive the biological tissue to rotate, thereby increasing the transparency of the device.

[0044] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0045] Finally, it should be noted that the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A biological tissue transparent processing device, comprising a frame (1), a chip (5), a peristaltic pump (7), and a reactor (8), characterized in that: The interior of the frame (1) is divided into two layers, the lower layer of the frame (1) is provided with a liquid tank (2) and a temperature control heating module (3), and the upper layer of the frame (1) is provided with an adjustable power supply (4), a chip (5), a refrigeration module (6), a peristaltic pump (7) and a reactor (8), the chip (5) is provided above the adjustable power supply (4), the reactor (8) is provided at the center of the frame (1), the upper end of the reactor (8) is provided with a rotating cap structure, the lower end of the rotating cap structure is provided with a reaction filtering mechanism, the reaction filtering mechanism is provided with a water inlet circulation mechanism, the lower end of the reactor (8) is provided with a water inlet mechanism, and the side of the frame (1) is provided with a suction separation mechanism; The reaction filtering mechanism comprises a reaction barrel (13) and a filter layer (14); the reaction barrel (13) is threadedly connected to the rotating cap structure; the interior of the reaction barrel (13) is hollow; the lower end of the reaction barrel (13) is arc-shaped; the side of the reaction barrel (13) is provided with a filter layer (14); and the lower end side of the reaction barrel (13) is provided with a baffle (18) at an equal angle; The water inlet circulation mechanism comprises a water inlet pipe (15), a fixing frame (16) and a drainage hole (17); the water inlet pipe (15) is arranged at equal angles inside the reaction barrel (13); one end of the water inlet pipe (15) is arranged on the side of the reaction barrel (13), and the water inlet pipe (15) is located at the lower end of the filter layer (14); and the other end of the water inlet pipe (15) is connected to the fixing frame (16); the fixing frame (16) is cylindrical as a whole, the upper end of the fixing frame (16) is concave, and the drainage holes (17) are evenly distributed inside the upper end of the fixing frame (16); The water inlet mechanism is embedded in the interior of the reaction and filtering mechanism, and comprises a water inlet pipe (19) and a fixed channel (20). The water inlet pipe (19) is arranged at the bottom of the reaction kettle (8), and an arc-shaped fixed channel (20) is arranged at an equal angle inside the upper end of the water inlet pipe (19); The bottom of the reactor (8) is provided with a negative electrode rod (21), and the attraction and separation mechanism comprises a positive electrode rod (22), an attraction layer (23), a connecting rod (24), a fixed gear (25), a connecting gear (26) and a motor (27). The positive electrode rod (22) is provided on the side of the reactor (8), and an annular attraction layer (23) is provided inside the reactor (8) for rotation. The attraction layer (23) and the positive electrode rod (22) are fitted and connected. The upper and lower ends of the attraction layer (23) are respectively provided with connecting rods (24), and the side of the upper end of the connecting rod (24) is provided with a fixed gear (25). The fixed gear (25) and the connecting gear (26) are meshed and connected. The connecting gear (26) is connected to the motor (27), and the motor (27) is provided on the outside of the reactor (8); A fixing ring (28) is provided on the side of the connecting rod (24) at the lower end, and an inclined guide layer (29) is provided on the side of the fixing ring (28).

2. The biological tissue transparent processing device according to claim 1, characterized in that: The rotating cap structure comprises a fixed cover (9), a locking rod (10), a connecting column (11) and a fixed cap (12); a rubber sleeve is provided at the lower end of the fixed cover (9); the fixed cover (9) is embedded in the interior of the reactor (8); a locking rod (10) is slidably provided at the upper end of the interior of the fixed cover (9); a connecting column (11) is rotatably provided at the lower end of the fixed cover (9); a fixed cap (12) is provided at the lower end of the connecting column (11); the fixed cap (12) is threadedly connected to the reaction filtering mechanism; the locking rod (10) and the connecting column (11) are snap-fitted.

3. The method for processing a biological tissue transparent processing device according to claim 1, comprising: a. Open the lid on the top of the rack (1), open the rotating cap structure of the reactor (8), place the tissue into the reactor (8), and then lock the sealing cover tightly; b. Turn on the power and start the machine automatically; c. Add 1L of reaction liquid to the liquid tank (2). The peristaltic pump (7) automatically draws the reaction liquid. At the same time, the temperature control heating module (3) and the refrigeration module (6) adjust the temperature of the reactor (8). d. Use physical methods and electricity to accelerate the combination and shedding speed of the reaction solution and fat, achieving tissue transparency within 3-5 days; e. The transparent tissue after the reaction is completed is taken out from the reactor (8) and the device is cleaned at the same time.

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