A high-activity microbial sewage treatment agent and preparation method thereof

Abstract

The invention discloses a high-activity microbial sewage treatment agent and a preparation method thereof, comprising the following components: microbial agent, organically modified diatomite, and acrylamide-containing polymer. The invention synthesizes organically modified diatomite through diatomite, lignin cationic surfactant, and olefin monomer, which can improve the loading capacity of the subsequent treatment agent for microorganisms; synthesizes acrylamide-containing polymer through epichlorohydrin, polyethyleneimine, acrylamide monomer, and sodium acrylate to form a three-dimensional network structure, which is conducive to the flocculation and sedimentation of impurities, has good surface activity and low surface tension, is more conducive to the attachment of microorganisms, and provides a basis for the high activity of microorganisms; the synthesis of the two provides more hydrophobic environment, and the overall alkaline characteristics, and the polymer therein is anionic, which can alleviate the acidic environment of the microorganisms loaded by the treatment agent, load the microbial agent, and the formed treatment agent is conducive to the loading and activity expression of the microorganisms on the prepared treatment agent.

Description

Technical Field

[0001] The invention relates to the technical field of water treatment, in particular to a high-activity microbial sewage treatment agent and a preparation method thereof. Background Art

[0002] In industrial production and daily life, due to human and mechanical activities and operation, the water resources used are polluted, and a large amount of wastewater is generated and discharged. It cannot be directly discharged into the natural environment and needs water treatment. Water treatment methods include physical treatment, chemical treatment, and sewage biochemical treatment. Among them, sewage biochemical treatment is to decompose organic matter and proliferate organisms through biological action, convert organic pollutants into harmless gases, liquids, and solids rich in organic matter, and then separate solids and liquids to remove non-settled suspended matter and soluble biodegradable organic matter. Take the soy product wastewater in food industry production as an example. The soy product has a high protein content and is mainly composed of amino acids. It will produce a large amount of organic wastewater, which is suitable for treatment by biochemical treatment technology. Some existing biochemical treatments use microorganisms as biological sources and load them on chitosan, sodium alginate and other materials to treat sewage, but the fixation performance of microorganisms is poor, which makes the continuous activity of microorganisms not high. Therefore, we propose a highly active microbial sewage treatment agent and its preparation method. Summary of the invention

[0003] The object of the present invention is to provide a highly active microbial sewage treatment agent and a preparation method thereof to solve the problems raised in the above background technology.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions: a highly active microbial sewage treatment agent, comprising the following components by weight: 18 to 27 parts of microbial agents, 20 to 38 parts of organically modified diatomaceous earth, and 10 to 20 parts of acrylamide-containing polymers.

[0005] Furthermore, the organic modified diatomaceous earth is prepared from the following components by weight: 20 to 36 parts of diatomaceous earth, 1 to 1.5 parts of lignin cationic surfactant, and 15 to 20 parts of olefin ester monomer.

[0006] Furthermore, the acrylamide-containing polymer is prepared from the following components by weight: 5 to 30 parts of epichlorohydrin, 50 parts of polyethyleneimine, 35 to 50 parts of acrylamide monomers, and 4 to 30 parts of sodium acrylate.

[0007] Furthermore, the acrylamide monomer is one or more of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0008] Furthermore, the olefin monomer is one or more of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate, and 2-ethoxyethyl acrylate.

[0009] Furthermore, the polyethyleneimine is branched polyethyleneimine.

[0010] A method for preparing a highly active microbial sewage treatment agent comprises the following steps:

[0011] (1) Preparation of acrylamide-containing polymers:

[0012] Epichlorohydrin, polyethyleneimine and a catalyst are mixed, sodium hydroxide is added, the mixture is stirred for reaction, and the mixture is allowed to stand to obtain product A;

[0013] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. A reduction-oxidation initiator, an emulsifier, and a solvent are added, and the mixture is evenly mixed. The temperature of the system is raised to react, and acrylamide monomer is added again, and water and an initiator are added to react to obtain product B.

[0014] Take an initiator, add deionized water, heat, slowly add product A and product B, keep the temperature to react, stop heating, stir and cool to room temperature to obtain an acrylamide-containing polymer;

[0015] (2) Preparation of organic modified diatomaceous earth:

[0016] Taking diatomaceous earth and lignin cationic surfactant, mixing, high-speed stirring, washing, and preparing organic diatomaceous earth;

[0017] Mixing organic diatomite and olefin ester monomer, adding solvent and initiator, heating and reacting to obtain organic modified diatomite;

[0018] (3) Preparation of treatment agent:

[0019] The organic modified diatomaceous earth and the acrylamide-containing polymer are ultrasonically treated respectively, the two are mixed, ultrasonically treated, and mixed with a microbial agent to prepare a treatment agent.

[0020] Furthermore, the step (1) includes the following steps:

[0021] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0022] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added, and the mixture is evenly mixed. The temperature of the system is raised to 40-50° C., and the mixture is reacted for 4-5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The mixture is reacted continuously, filtered, and vacuum dried to obtain product B.

[0023] Take sodium bisulfite, add deionized water to prepare a solution, heat to 70-80°C, slowly add product A and product B, maintain the temperature for reaction for 4-5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer.

[0024] Furthermore, the step (2) includes the following steps:

[0025] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6 to 7 hours, washing, and drying to obtain organic diatomaceous earth;

[0026] Take organic diatomaceous earth and olefin monomer, mix them, add acetone, acetic acid and potassium persulfate, heat to 70-90° C., react for 2-6 hours, filter and vacuum dry to obtain organic modified diatomaceous earth.

[0027] Furthermore, the step (2) includes the following steps:

[0028] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6 to 7 hours, washing, and drying to obtain organic diatomaceous earth;

[0029] Take organic diatomaceous earth and olefin monomer, mix them, add acetone, acetic acid and potassium persulfate, heat to 70-90° C., react for 2-6 hours, filter and vacuum dry to obtain organic modified diatomaceous earth.

[0030] In the above technical scheme, the epoxy group in epichlorohydrin reacts with the amino group of polyethyleneimine under the action of benzyltriethylammonium chloride, and the product eliminates the chlorine group under the action of sodium hydroxide to generate epoxy group again to obtain product A; sodium acrylate and acrylamide monomers are synthesized by emulsion polymerization, and then reacted with acrylamide monomer to obtain a polymer of sodium acrylate and acrylamide to obtain product B; product A and product B are blended and polymerized under the action of an initiator to obtain an acrylamide-containing polymer, which can promote flocculation of impurities in sewage;

[0031] Diatomaceous earth is mixed with a lignin cationic surfactant to obtain organic diatomaceous earth, the adsorption capacity of the diatomaceous earth is improved, and after the olefinic ester monomer is adsorbed, it is polymerized in the pores of the diatomaceous earth to obtain organic modified diatomaceous earth; the acrylamide-containing polymer and the polymer in the organic modified diatom are hydrolyzed and copolymerized under ultrasonic action, and mixed with a microbial agent to obtain a treatment agent;

[0032] The polymers mentioned above have both hydrophilic groups and hydrophobic groups. When the prepared treatment agent is in a sewage system, the polymers are interconnected to form a uniform three-dimensional network structure covering the entire system, which is conducive to capturing impurities in the sewage, accelerating the flocculation and sedimentation of impurities, and has good temperature and salt resistance. In addition, the polymers have good surface activity and low surface tension, which reduces the surface tension of the treatment agent, is more conducive to the attachment of microorganisms, and provides a basis for the high activity of microorganisms.

[0033] The polymer is combined with organically modified diatomaceous earth to provide a more hydrophobic environment, which is more conducive to the attachment of microorganisms to the treatment agent. Since the degradation of organic matter in sewage will produce organic acids, the pH of the system will be lowered, which is not conducive to the growth of microorganisms. The treatment agent is alkaline as a whole, and the polymer in it is anionic, which can alleviate the environment in which the microorganisms loaded by the treatment agent are located and improve the activity of microorganisms.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The high-activity microbial sewage treatment agent and the preparation method thereof of the present invention are as follows: organic modified diatomaceous earth is synthesized by diatomaceous earth, lignin cationic surfactant and olefin ester monomer, so that the loading capacity of the treatment agent for microorganisms can be improved; acrylamide-containing polymer is synthesized by epichlorohydrin, polyethyleneimine, acrylamide monomer and sodium acrylate; each polymer is interconnected to form a three-dimensional network structure, which is conducive to capturing impurities in sewage and accelerating the flocculation and sedimentation of impurities; the polymer has good surface activity and low surface tension, is more conducive to the attachment of microorganisms, and provides a basis for the high activity of microorganisms; the synthesis of the two provides more hydrophobic environment, and the overall alkaline characteristics; the polymer is anionic, which can alleviate the acidic environment of the microorganisms loaded by the treatment agent, load the microbial agent, and the formed treatment agent is conducive to the loading and activity expression of the microorganisms on the prepared treatment agent. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Example 1

[0038] (1) Preparation of acrylamide-containing polymers:

[0039] The acrylamide-containing polymer is prepared from the following components by weight: 18 parts of epichlorohydrin, 50 parts of polyethyleneimine, 42 parts of acrylamide monomer, and 17 parts of sodium acrylate;

[0040] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0041] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 40° C. and the reaction is carried out for 4 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0042] Take sodium bisulfite, add deionized water to prepare a solution, heat to 70°C, slowly add product A and product B, keep the temperature to react for 4 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0043] (2) Preparation of organic modified diatomaceous earth:

[0044] The organic modified diatomaceous earth is prepared from the following components by weight: 28 parts of diatomaceous earth, 1.2 parts of lignin cationic surfactant, and 17 parts of olefin ester monomer;

[0045] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6 hours, washing, and drying to obtain organic diatomaceous earth;

[0046] Take organic diatomite and olefin monomer, mix them, add acetone, acetic acid and potassium persulfate, heat to 70°C and react for 2h, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0047] (3) Preparation of treatment agent:

[0048] The treatment agent includes the following components by weight: 23 parts of microbial agent, 29 parts of organic modified diatomaceous earth, and 15 parts of acrylamide-containing polymer;

[0049] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 50°C, and mixed with a microbial agent to prepare a treatment agent.

[0050] Example 2

[0051] (1) Preparation of acrylamide-containing polymers:

[0052] The acrylamide-containing polymer is prepared from the following components by weight: 18 parts of epichlorohydrin, 50 parts of polyethyleneimine, 42 parts of acrylamide monomer, and 17 parts of sodium acrylate;

[0053] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0054] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 45° C. and the reaction is carried out for 4.5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0055] Take sodium bisulfite, add deionized water to prepare a solution, heat to 75°C, slowly add product A and product B, keep the temperature to react for 4.5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0056] (2) Preparation of organic modified diatomaceous earth:

[0057] The organic modified diatomaceous earth is prepared from the following components by weight: 28 parts of diatomaceous earth, 1.2 parts of lignin cationic surfactant, and 17 parts of olefin ester monomer;

[0058] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0059] Mix organic diatomite and olefin monomer, add acetone, acetic acid and potassium persulfate, heat to 80°C for 4 hours, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0060] (3) Preparation of treatment agent:

[0061] The treatment agent includes the following components by weight: 23 parts of microbial agent, 29 parts of organic modified diatomaceous earth, and 15 parts of acrylamide-containing polymer;

[0062] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55° C., and mixed with a microbial agent to prepare a treatment agent.

[0063] Example 3

[0064] (1) Preparation of acrylamide-containing polymers:

[0065] The acrylamide-containing polymer is prepared from the following components by weight: 18 parts of epichlorohydrin, 50 parts of polyethyleneimine, 42 parts of acrylamide monomer, and 17 parts of sodium acrylate;

[0066] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0067] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 50° C. and the reaction is carried out for 5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0068] Take sodium bisulfite, add deionized water to prepare a solution, heat to 80°C, slowly add product A and product B, keep the temperature to react for 5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0069] (2) Preparation of organic modified diatomaceous earth:

[0070] The organic modified diatomaceous earth is prepared from the following components by weight: 28 parts of diatomaceous earth, 1.2 parts of lignin cationic surfactant, and 17 parts of olefin ester monomer;

[0071] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 7 hours, washing, and drying to obtain organic diatomaceous earth;

[0072] Mixing organic diatomite and olefin monomer, adding acetone, acetic acid and potassium persulfate, heating to 90°C for 6 hours, filtering and vacuum drying to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0073] (3) Preparation of treatment agent:

[0074] The treatment agent includes the following components by weight: 23 parts of microbial agent, 29 parts of organic modified diatomaceous earth, and 15 parts of acrylamide-containing polymer;

[0075] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 60°C, and mixed with a microbial agent to prepare a treatment agent.

[0076] Example 4

[0077] (1) Preparation of acrylamide-containing polymers:

[0078] The acrylamide-containing polymer is prepared from the following components by weight: 18 parts of epichlorohydrin, 50 parts of polyethyleneimine, 42 parts of acrylamide monomer, and 17 parts of sodium acrylate;

[0079] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0080] Sodium acrylate and acrylamide were blended, sodium dodecyl sulfate was added, and the mixture was stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate were added, and the mixture was evenly mixed. The temperature of the system was raised to 45° C., and the mixture was reacted for 4.5 hours. Acrylamide monomer was added again, and water and sodium bisulfite were added. The mixture was reacted again, and the mixture was filtered and dried under vacuum to obtain product B.

[0081] Take sodium bisulfite, add deionized water to prepare a solution, heat to 75°C, slowly add product A and product B, keep the temperature to react for 4.5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0082] (2) Preparation of organic modified diatomaceous earth:

[0083] The organic modified diatomaceous earth is prepared from the following components by weight: 28 parts of diatomaceous earth, 1.2 parts of lignin cationic surfactant, and 17 parts of olefin ester monomer;

[0084] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0085] Take organic diatomite and vinyl acetate, mix them, add acetone, acetic acid and potassium persulfate, heat to 80°C and react for 4 hours, filter and vacuum dry to obtain organic modified diatomite;

[0086] (3) Preparation of treatment agent:

[0087] The treatment agent includes the following components by weight: 23 parts of microbial agent, 29 parts of organic modified diatomaceous earth, and 15 parts of acrylamide-containing polymer;

[0088] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55° C., and mixed with a microbial agent to prepare a treatment agent.

[0089] Example 5

[0090] (1) Preparation of acrylamide-containing polymers:

[0091] The acrylamide-containing polymer is prepared from the following components by weight: 5 parts of epichlorohydrin, 50 parts of polyethyleneimine, 35 parts of acrylamide monomer, and 4 parts of sodium acrylate;

[0092] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0093] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 45° C. and the reaction is carried out for 4.5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0094] Take sodium bisulfite, add deionized water to prepare a solution, heat to 75°C, slowly add product A and product B, keep the temperature to react for 4.5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0095] (2) Preparation of organic modified diatomaceous earth:

[0096] The organic modified diatomaceous earth is prepared from the following components by weight: 20 parts of diatomaceous earth, 1 part of lignin cationic surfactant, and 15 parts of olefin ester monomer;

[0097] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0098] Mix organic diatomite and olefin monomer, add acetone, acetic acid and potassium persulfate, heat to 80°C for 4 hours, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0099] (3) Preparation of treatment agent:

[0100] The treatment agent includes the following components by weight: 18 parts of microbial agent, 20 parts of organic modified diatomaceous earth, and 10 parts of acrylamide-containing polymer;

[0101] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55° C., and mixed with a microbial agent to prepare a treatment agent.

[0102] Example 6

[0103] (1) Preparation of acrylamide-containing polymers:

[0104] The acrylamide-containing polymer is prepared from the following components by weight: 30 parts of epichlorohydrin, 50 parts of polyethyleneimine, 50 parts of acrylamide monomer, and 30 parts of sodium acrylate;

[0105] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0106] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 45° C. and the reaction is carried out for 4.5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0107] Take sodium bisulfite, add deionized water to prepare a solution, heat to 75°C, slowly add product A and product B, keep the temperature to react for 4.5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0108] (2) Preparation of organic modified diatomaceous earth:

[0109] The organic modified diatomaceous earth is prepared from the following components by weight: 36 parts of diatomaceous earth, 1.5 parts of lignin cationic surfactant, and 20 parts of olefin ester monomer;

[0110] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0111] Mix organic diatomite and olefin monomer, add acetone, acetic acid and potassium persulfate, heat to 80°C for 4 hours, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0112] (3) Preparation of treatment agent:

[0113] The treatment agent includes the following components by weight: 27 parts of microbial agent, 38 parts of organic modified diatomaceous earth, and 20 parts of acrylamide-containing polymer;

[0114] The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55° C., and mixed with a microbial agent to prepare a treatment agent.

[0115] Comparative Example 1

[0116] (1) Preparation of organic modified diatomaceous earth:

[0117] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0118] Mix organic diatomite and olefin monomer, add acetone, acetic acid and potassium persulfate, heat to 80°C for 4 hours, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0119] (2) Preparation of treatment agent:

[0120] The organic modified diatomaceous earth is mixed with a microbial agent to prepare a treatment agent.

[0121] Comparative Example 2

[0122] (1) Preparation of acrylamide-containing polymers:

[0123] Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A;

[0124] Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added and mixed evenly. The system temperature is raised to 45° C. and the reaction is carried out for 4.5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The reaction is continued, filtered, and vacuum dried to obtain product B; wherein the acrylamide monomer is a mixture of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide, and diacetone acrylamide.

[0125] Take sodium bisulfite, add deionized water to prepare a solution, heat to 75°C, slowly add product A and product B, keep the temperature to react for 4.5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer;

[0126] (2) Preparation of treatment agent:

[0127] Diatomaceous earth and acrylamide-containing polymer are placed in ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55° C., and mixed with a microbial agent to prepare a treatment agent.

[0128] Comparative Example 3

[0129] (1) Preparation of organic modified diatomaceous earth:

[0130] Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6.5 hours, washing, and drying, to obtain organic diatomaceous earth;

[0131] Mix organic diatomite and olefin monomer, add acetone, acetic acid and potassium persulfate, heat to 80°C for 4 hours, filter and vacuum dry to obtain organic modified diatomite; wherein the olefin monomer is a mixture of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate and 2-ethoxyethyl acrylate;

[0132] (2) Preparation of treatment agent:

[0133] The organic modified diatomaceous earth and polyethyleneimine, polyacrylamide and sodium polyacrylate polymer are placed in ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 55°C, and mixed with microbial agents to prepare a treatment agent.

[0134] Comparative Example 4

[0135] Take diatomaceous earth and mix it with microbial agent to prepare the treatment agent.

[0136] experiment

[0137] The treatment agents and pure diatomaceous earth obtained in Examples 1-6 and Comparative Examples 1-4 were used to prepare samples, and their water treatment performance was tested and the test results were recorded:

[0138] Water treatment performance test: COD, total nitrogen and ammonia nitrogen removal rates after the treatment agent was used in bean product wastewater for 30 days;

[0139]

[0140] According to the data in the above table, we can clearly draw the following conclusions:

[0141] The treatment agents obtained in Examples 1-6 and Comparative Examples 1-4 and pure diatomaceous earth are compared, and the test results show that:

[0142] 1. Compared with the treatment agent obtained in Comparative Example 4, the treatment agents obtained in Examples 1-6 have different implementation parameters in Examples 1-3, the selection of monomers in Example 4, and the component ratios in Examples 5-6 are different from those in Example 2, and the COD, total nitrogen, and ammonia nitrogen removal rate data thereof are significantly increased, which fully demonstrates that the present invention achieves an improvement in the water treatment performance of the treatment agent and can maintain the high activity of the microorganisms in the treatment agent;

[0143] 2. The treatment agent obtained in Example 2 is compared with the treatment agents obtained in Comparative Examples 1-3, wherein no acrylamide-containing polymer is added in Comparative Example 1, the diatomaceous earth is not modified in Comparative Example 2, and the acrylamide-containing polymer is replaced with a single-component polymer in Comparative Example 3. The treatment agents obtained in Comparative Examples 1-3 have decreased COD, total nitrogen, and ammonia nitrogen removal rate data. It can be seen that the treatment process of diatomaceous earth can improve the sewage treatment capacity of the prepared treatment agent and maintain the high activity of the microorganisms in the treatment agent.

[0144] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process method article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process method article or device.

[0145] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a highly active microbial sewage treatment agent, characterized in that: The following steps are involved: (1) Preparation of acrylamide-containing polymers: Epichlorohydrin, polyethyleneimine and a catalyst are mixed, sodium hydroxide is added, the mixture is stirred for reaction, and the mixture is allowed to stand to obtain product A; Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. A reduction-oxidation initiator, an emulsifier, and a solvent are added, and the mixture is evenly mixed. The temperature of the system is raised to react, and acrylamide monomer is added again, and water and an initiator are added to react to obtain product B. Take an initiator, add deionized water, heat, slowly add product A and product B, keep the temperature to react, stop heating, stir and cool to room temperature to obtain an acrylamide-containing polymer; (2) Preparation of organic modified diatomaceous earth: Taking diatomaceous earth and lignin cationic surfactant, mixing, high-speed stirring, washing, and preparing organic diatomaceous earth; Mixing organic diatomite and olefin ester monomer, adding solvent and initiator, heating and reacting to obtain organic modified diatomite; (3) Preparation of treatment agent: Taking organic modified diatomaceous earth and acrylamide-containing polymer, ultrasonically treating them respectively, mixing the two, ultrasonically treating them, and mixing them with a microbial agent to prepare a treatment agent; The treatment agent comprises the following components by weight: 18 to 27 parts of microbial agents, 20 to 38 parts of organic modified diatomaceous earth, and 10 to 20 parts of acrylamide-containing polymers.

2. The method for preparing a highly active microbial sewage treatment agent according to claim 1, characterized in that: The step (1) comprises the following steps: Epichlorohydrin, polyethyleneimine and benzyltriethylammonium chloride were mixed, sodium hydroxide was added, stirred for reaction for 1 hour, allowed to stand, filtered, washed, and vacuum dried to obtain product A; Sodium acrylate and acrylamide monomers are blended, sodium dodecyl sulfate is added, and the mixture is stirred in a nitrogen atmosphere. Ammonium persulfate, sodium bisulfite, acryloxy span-80, and sodium acetate are added, and the mixture is evenly mixed. The temperature of the system is raised to 40-50° C., and the mixture is reacted for 4-5 hours. Acrylamide monomer is added again, and water and sodium bisulfite are added. The mixture is reacted continuously, filtered, and vacuum dried to obtain product B. Take sodium bisulfite, add deionized water to prepare a solution, heat to 70-80°C, slowly add product A and product B, maintain the temperature for reaction for 4-5 hours, stop heating, stir and cool to room temperature to prepare an acrylamide-containing polymer.

3. The method for preparing a highly active microbial sewage treatment agent according to claim 1, characterized in that: The step (2) comprises the following steps: Taking diatomaceous earth and lignin cationic surfactant and mixing them, stirring at high speed for 6 to 7 hours, washing, and drying to obtain organic diatomaceous earth; Take organic diatomaceous earth and olefin monomer, mix them, add acetone, acetic acid and potassium persulfate, heat to 70-90° C., react for 2-6 hours, filter and vacuum dry to obtain organic modified diatomaceous earth.

4. The method for preparing a highly active microbial sewage treatment agent according to claim 1, characterized in that: The step (3) comprises the following steps: The organic modified diatomaceous earth and the acrylamide-containing polymer are placed in an ethanol aqueous solution for ultrasonic treatment respectively, the two are mixed, ultrasonically treated, concentrated under reduced pressure, vacuum dried at 50-60° C., and mixed with a microbial agent to prepare a treatment agent.

5. The method for preparing a highly active microbial sewage treatment agent according to claim 1, characterized in that: The organic modified diatomaceous earth is prepared from the following components by weight: 20 to 36 parts of diatomaceous earth, 1 to 1.5 parts of lignin cationic surfactant, and 15 to 20 parts of olefin ester monomer.

6. The method for preparing a highly active microbial sewage treatment agent according to claim 1, characterized in that: The acrylamide-containing polymer is prepared from the following components by weight: 5 to 30 parts of epichlorohydrin, 50 parts of polyethyleneimine, 35 to 50 parts of acrylamide monomers, and 4 to 30 parts of sodium acrylate.

7. The method for preparing a highly active microbial sewage treatment agent according to claim 6, characterized in that: The acrylamide monomer is one or more of acrylamide, N-isopropyl methacrylamide, dimethylaminopropyl acrylamide, methacrylamide and diacetone acrylamide.

8. The method for preparing a highly active microbial sewage treatment agent according to claim 5, characterized in that: The olefin monomer is one or more of vinyl acetate, hydroxyethyl acrylate, vinyl ethylene carbonate, and 2-ethoxyethyl acrylate.

9. The method for preparing a highly active microbial sewage treatment agent according to claim 6, characterized in that: The polyethyleneimine is branched polyethyleneimine.

10. A highly active microbial sewage treatment agent prepared according to the preparation method according to any one of claims 1 to 9.

Images